Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33491.t1 | XP_003604643.1 | 92.1 | 76 | 6 | 0 | 1 | 76 | 28 | 103 | 1.90E-32 | 147.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33491.t1 | Q84YK7 | 70.5 | 61 | 18 | 0 | 16 | 76 | 25 | 85 | 4.2e-20 | 98.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33491.t1 | A8TVQ0 | 92.1 | 76 | 6 | 0 | 1 | 76 | 28 | 103 | 1.4e-32 | 147.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057261 | MS.gene33491 | 0.803075 | 4.14E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAAAACGTACCTGGTAAT+TGG | 0.306037 | 4.3:-83038916 | None:intergenic |
| AAATATATATACCTGGTGTA+TGG | 0.360290 | 4.3:-83039448 | None:intergenic |
| CATGAAGACGGAAGAGGAAT+TGG | 0.391004 | 4.3:+83039407 | MS.gene33491:CDS |
| TGATCACTATCATCGCTATA+AGG | 0.396584 | 4.3:+83040318 | MS.gene33491:CDS |
| GCATAAATAGAGGCAATAAC+AGG | 0.437739 | 4.3:-83038831 | None:intergenic |
| AATATATATACCTGGTGTAT+GGG | 0.492563 | 4.3:-83039447 | None:intergenic |
| AAAACTGTCAGCATAAATAG+AGG | 0.520509 | 4.3:-83038841 | None:intergenic |
| TACTGGCTCCTCCAATTACC+AGG | 0.536244 | 4.3:+83038905 | MS.gene33491:CDS |
| ACGGAAGAGGAATTGGTACA+TGG | 0.537410 | 4.3:+83039414 | MS.gene33491:CDS |
| ATTGCATACAGGGAAGGTAA+AGG | 0.557945 | 4.3:+83040270 | MS.gene33491:intron |
| GCTGTACATGAAGACGGAAG+AGG | 0.569110 | 4.3:+83039401 | MS.gene33491:CDS |
| CATACAGGGAAGGTAAAGGA+TGG | 0.571680 | 4.3:+83040274 | MS.gene33491:intron |
| GTGTTGATAATGTAGTATGA+AGG | 0.577599 | 4.3:+83039377 | MS.gene33491:intron |
| GATATATTTGCCCATACACC+AGG | 0.579641 | 4.3:+83039437 | MS.gene33491:CDS |
| AAAACGTACCTGGTAATTGG+AGG | 0.599628 | 4.3:-83038913 | None:intergenic |
| GAAGGTGCTGTACATGAAGA+CGG | 0.631814 | 4.3:+83039395 | MS.gene33491:CDS |
| CGGAAGAGGAATTGGTACAT+GGG | 0.654494 | 4.3:+83039415 | MS.gene33491:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAAAAAATATATATACC+TGG | - | chr4.3:83039458-83039477 | None:intergenic | 15.0% |
| !!! | AATAGTTTTTTTTACACTAT+CGG | - | chr4.3:83040071-83040090 | None:intergenic | 15.0% |
| !!! | GGAATTAAACATATTTTTTT+TGG | - | chr4.3:83039134-83039153 | None:intergenic | 15.0% |
| !!! | TTTATTTTTATAGCTGTATA+TGG | - | chr4.3:83039576-83039595 | None:intergenic | 15.0% |
| !! | AAAAAAAAATTGAAGGTCTT+GGG | - | chr4.3:83039840-83039859 | None:intergenic | 20.0% |
| !! | AGTAGATTGATTATAATTGA+CGG | - | chr4.3:83039894-83039913 | None:intergenic | 20.0% |
| !! | ATATAAATCATCTGAAAGTT+TGG | - | chr4.3:83039937-83039956 | None:intergenic | 20.0% |
| !! | CAAAGAGAAAAAAAAATTGA+AGG | - | chr4.3:83039847-83039866 | None:intergenic | 20.0% |
| !! | TATTGAATCTTAAGAATTTG+AGG | + | chr4.3:83039339-83039358 | MS.gene33491:intron | 20.0% |
| !!! | CTTCAATTTTTTTTTCTCTT+TGG | + | chr4.3:83039845-83039864 | MS.gene33491:intron | 20.0% |
| !!! | TCTTTTAATCTTTTCCAATT+TGG | + | chr4.3:83039033-83039052 | MS.gene33491:intron | 20.0% |
| !!! | TCTTTTCTTATATTTTAGAC+CGG | - | chr4.3:83039245-83039264 | None:intergenic | 20.0% |
| !!! | TTTAAGACATGTTTTTTCTT+TGG | - | chr4.3:83039174-83039193 | None:intergenic | 20.0% |
| !!! | TTTCTATTTCTCTAAATTTC+TGG | - | chr4.3:83039210-83039229 | None:intergenic | 20.0% |
| ! | AAAGATTAAAAGAACGAAGA+TGG | - | chr4.3:83039026-83039045 | None:intergenic | 25.0% |
| ! | AAATATATATACCTGGTGTA+TGG | - | chr4.3:83039451-83039470 | None:intergenic | 25.0% |
| ! | AATATATATACCTGGTGTAT+GGG | - | chr4.3:83039450-83039469 | None:intergenic | 25.0% |
| ! | AATTCCTAATGCTATAAGAT+TGG | + | chr4.3:83039148-83039167 | MS.gene33491:intron | 25.0% |
| ! | AATTGTTGTAATTGCATACA+GGG | + | chr4.3:83040260-83040279 | MS.gene33491:intron | 25.0% |
| ! | GAAAAAAAAATTGAAGGTCT+TGG | - | chr4.3:83039841-83039860 | None:intergenic | 25.0% |
| ! | TAATTGTTGTAATTGCATAC+AGG | + | chr4.3:83040259-83040278 | MS.gene33491:intron | 25.0% |
| !! | ATTTTTATAGCTGTATATGG+TGG | - | chr4.3:83039573-83039592 | None:intergenic | 25.0% |
| !! | GCTTCACATTTTTATCATAA+AGG | + | chr4.3:83038983-83039002 | MS.gene33491:intron | 25.0% |
| !! | TCTTTTCTTCAATATCTAAC+AGG | - | chr4.3:83040153-83040172 | None:intergenic | 25.0% |
| !!! | ATAGAAAGTAATGCTTTTTC+CGG | + | chr4.3:83039223-83039242 | MS.gene33491:intron | 25.0% |
| !!! | TATGCTTTTGCTTTTTGTAA+CGG | + | chr4.3:83039101-83039120 | MS.gene33491:intron | 25.0% |
| AAAACTGTCAGCATAAATAG+AGG | - | chr4.3:83038844-83038863 | None:intergenic | 30.0% | |
| ACCACCAAAAAAAATTCCTA+AGG | - | chr4.3:83039643-83039662 | None:intergenic | 30.0% | |
| GACAATGCATACTATATACA+AGG | - | chr4.3:83039687-83039706 | None:intergenic | 30.0% | |
| ! | CTTCATACTTTTGTCCAAAT+TGG | - | chr4.3:83039050-83039069 | None:intergenic | 30.0% |
| !! | GTGTTGATAATGTAGTATGA+AGG | + | chr4.3:83039377-83039396 | MS.gene33491:intron | 30.0% |
| !!! | CTTGCCTTAGGAATTTTTTT+TGG | + | chr4.3:83039636-83039655 | MS.gene33491:intron | 30.0% |
| !!! | TTAGGAATTTTTTTTGGTGG+TGG | + | chr4.3:83039642-83039661 | MS.gene33491:intron | 30.0% |
| ACAATGTTACAAAACGTACC+TGG | - | chr4.3:83038926-83038945 | None:intergenic | 35.0% | |
| ACATGAACATATCTTGCCTT+AGG | + | chr4.3:83039624-83039643 | MS.gene33491:intron | 35.0% | |
| ACGGTATGAGTAGTTCAATA+GGG | - | chr4.3:83039875-83039894 | None:intergenic | 35.0% | |
| ATCACTATCATCGCTATAAG+GGG | + | chr4.3:83040320-83040339 | MS.gene33491:CDS | 35.0% | |
| CATACTATATACAAGGTCTG+GGG | - | chr4.3:83039680-83039699 | None:intergenic | 35.0% | |
| GATCACTATCATCGCTATAA+GGG | + | chr4.3:83040319-83040338 | MS.gene33491:CDS | 35.0% | |
| GCATAAATAGAGGCAATAAC+AGG | - | chr4.3:83038834-83038853 | None:intergenic | 35.0% | |
| GCATACTATATACAAGGTCT+GGG | - | chr4.3:83039681-83039700 | None:intergenic | 35.0% | |
| GTACCAAAAACAAAACCTTC+CGG | - | chr4.3:83038888-83038907 | None:intergenic | 35.0% | |
| TACAAAACGTACCTGGTAAT+TGG | - | chr4.3:83038919-83038938 | None:intergenic | 35.0% | |
| TGATCACTATCATCGCTATA+AGG | + | chr4.3:83040318-83040337 | MS.gene33491:CDS | 35.0% | |
| TGCATACTATATACAAGGTC+TGG | - | chr4.3:83039682-83039701 | None:intergenic | 35.0% | |
| TGTGTATGTTACTATGCATG+TGG | + | chr4.3:83038943-83038962 | MS.gene33491:intron | 35.0% | |
| TTCATGTAGCTTTCTCTATG+TGG | - | chr4.3:83039611-83039630 | None:intergenic | 35.0% | |
| TTGGCCAATCTTATAGCATT+AGG | - | chr4.3:83039155-83039174 | None:intergenic | 35.0% | |
| ! | AGAGTTTCTAGACAAGTCAT+AGG | + | chr4.3:83039991-83040010 | MS.gene33491:intron | 35.0% |
| ! | AGCTGTATATGGTGGTTTTT+AGG | - | chr4.3:83039565-83039584 | None:intergenic | 35.0% |
| ! | CTATCCACTATCTAGGTTTT+TGG | - | chr4.3:83039512-83039531 | None:intergenic | 35.0% |
| !! | CAAAAACCTAGATAGTGGAT+AGG | + | chr4.3:83039510-83039529 | MS.gene33491:intron | 35.0% |
| !!! | CTGACAGTTTTGAGCTAAAT+CGG | + | chr4.3:83038853-83038872 | MS.gene33491:CDS | 35.0% |
| !!! | GAAGGTTTTGTTTTTGGTAC+TGG | + | chr4.3:83038888-83038907 | MS.gene33491:CDS | 35.0% |
| !!! | GAATTTTTTTTGGTGGTGGT+CGG | + | chr4.3:83039646-83039665 | MS.gene33491:intron | 35.0% |
| !!! | GCCTTAGGAATTTTTTTTGG+TGG | + | chr4.3:83039639-83039658 | MS.gene33491:intron | 35.0% |
| !!! | TTTCCGGAAGGTTTTGTTTT+TGG | + | chr4.3:83038882-83038901 | MS.gene33491:CDS | 35.0% |
| AAAACGTACCTGGTAATTGG+AGG | - | chr4.3:83038916-83038935 | None:intergenic | 40.0% | |
| AAAAGAACGAAGATGGAGAG+AGG | - | chr4.3:83039019-83039038 | None:intergenic | 40.0% | |
| AAAGAACGAAGATGGAGAGA+GGG | - | chr4.3:83039018-83039037 | None:intergenic | 40.0% | |
| ACAACCATGTGAGTTTAGCT+CGG | - | chr4.3:83039761-83039780 | None:intergenic | 40.0% | |
| ATGGTTGTAGTGGTGTGATT+TGG | + | chr4.3:83039773-83039792 | MS.gene33491:intron | 40.0% | |
| ATTGCATACAGGGAAGGTAA+AGG | + | chr4.3:83040270-83040289 | MS.gene33491:intron | 40.0% | |
| CTAAACTCACATGGTTGTAG+TGG | + | chr4.3:83039763-83039782 | MS.gene33491:intron | 40.0% | |
| CTAGCCAAAAACCTAGATAG+TGG | + | chr4.3:83039505-83039524 | MS.gene33491:intron | 40.0% | |
| GACGGTATGAGTAGTTCAAT+AGG | - | chr4.3:83039876-83039895 | None:intergenic | 40.0% | |
| GATATATTTGCCCATACACC+AGG | + | chr4.3:83039437-83039456 | MS.gene33491:CDS | 40.0% | |
| GTTGTAATTGCATACAGGGA+AGG | + | chr4.3:83040264-83040283 | MS.gene33491:intron | 40.0% | |
| TAAACTCACAAGGACGTCTT+AGG | + | chr4.3:83039721-83039740 | MS.gene33491:intron | 40.0% | |
| TTGTAGCCTATCCACTATCT+AGG | - | chr4.3:83039519-83039538 | None:intergenic | 40.0% | |
| ! | TGAGTTTAGCTCAGTTGGTA+TGG | - | chr4.3:83039709-83039728 | None:intergenic | 40.0% |
| ! | TTGTAGTGGTGTGATTTGGA+CGG | + | chr4.3:83039777-83039796 | MS.gene33491:intron | 40.0% |
| !! | TATATGGTGGTTTTTAGGCG+TGG | - | chr4.3:83039560-83039579 | None:intergenic | 40.0% |
| AACTCTAATGCCACTCTACG+TGG | + | chr4.3:83040200-83040219 | MS.gene33491:intron | 45.0% | |
| ACGGAAGAGGAATTGGTACA+TGG | + | chr4.3:83039414-83039433 | MS.gene33491:CDS | 45.0% | |
| CATACAGGGAAGGTAAAGGA+TGG | + | chr4.3:83040274-83040293 | MS.gene33491:intron | 45.0% | |
| CATGAAGACGGAAGAGGAAT+TGG | + | chr4.3:83039407-83039426 | MS.gene33491:CDS | 45.0% | |
| CCAACTGAGCTAAACTCACA+AGG | + | chr4.3:83039711-83039730 | MS.gene33491:intron | 45.0% | |
| CGGAAGAGGAATTGGTACAT+GGG | + | chr4.3:83039415-83039434 | MS.gene33491:CDS | 45.0% | |
| ! | AGGCGTGGTCATGATTTGTA+AGG | - | chr4.3:83039545-83039564 | None:intergenic | 45.0% |
| ! | CCTTGTGAGTTTAGCTCAGT+TGG | - | chr4.3:83039714-83039733 | None:intergenic | 45.0% |
| ! | GCTAAATCGGAGCAGTTTTC+CGG | + | chr4.3:83038866-83038885 | MS.gene33491:CDS | 45.0% |
| !! | GAAGGTGCTGTACATGAAGA+CGG | + | chr4.3:83039395-83039414 | MS.gene33491:CDS | 45.0% |
| CTAGCCGAGCTAAACTCACA+TGG | + | chr4.3:83039754-83039773 | MS.gene33491:intron | 50.0% | |
| GCTGTACATGAAGACGGAAG+AGG | + | chr4.3:83039401-83039420 | MS.gene33491:CDS | 50.0% | |
| ! | AATCGGAGCAGTTTTCCGGA+AGG | + | chr4.3:83038870-83038889 | MS.gene33491:CDS | 50.0% |
| !! | TACTGGCTCCTCCAATTACC+AGG | + | chr4.3:83038905-83038924 | MS.gene33491:CDS | 50.0% |
| ATGCCACTCTACGTGGCTAC+TGG | + | chr4.3:83040207-83040226 | MS.gene33491:intron | 55.0% | |
| ! | GTGTGATTTGGACGGTGTCG+TGG | + | chr4.3:83039785-83039804 | MS.gene33491:intron | 55.0% |
| !! | CGGTGTCGTGGTGGAAACTT+TGG | + | chr4.3:83039797-83039816 | MS.gene33491:intron | 55.0% |
| !! | TGATTTGGACGGTGTCGTGG+TGG | + | chr4.3:83039788-83039807 | MS.gene33491:intron | 55.0% |
| CAGCCAGTAGCCACGTAGAG+TGG | - | chr4.3:83040213-83040232 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 83038823 | 83040339 | 83038823 | ID=MS.gene33491 |
| chr4.3 | mRNA | 83038823 | 83040339 | 83038823 | ID=MS.gene33491.t1;Parent=MS.gene33491 |
| chr4.3 | exon | 83038823 | 83038926 | 83038823 | ID=MS.gene33491.t1.exon1;Parent=MS.gene33491.t1 |
| chr4.3 | CDS | 83038823 | 83038926 | 83038823 | ID=cds.MS.gene33491.t1;Parent=MS.gene33491.t1 |
| chr4.3 | exon | 83039392 | 83039458 | 83039392 | ID=MS.gene33491.t1.exon2;Parent=MS.gene33491.t1 |
| chr4.3 | CDS | 83039392 | 83039458 | 83039392 | ID=cds.MS.gene33491.t1;Parent=MS.gene33491.t1 |
| chr4.3 | exon | 83040281 | 83040339 | 83040281 | ID=MS.gene33491.t1.exon3;Parent=MS.gene33491.t1 |
| chr4.3 | CDS | 83040281 | 83040339 | 83040281 | ID=cds.MS.gene33491.t1;Parent=MS.gene33491.t1 |
| Gene Sequence |
| Protein sequence |
