AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33659

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33659.t1 MTR_3g089055 94.513 565 31 0 1 565 6 570 0.0 1075
MS.gene33659.t1 MTR_3g089055 94.513 565 31 0 1 565 1 565 0.0 1074
MS.gene33659.t1 MTR_1g029420 69.950 599 139 7 1 565 1 592 0.0 793
MS.gene33659.t1 MTR_5g097480 50.962 416 198 1 156 565 129 544 9.40e-153 449
MS.gene33659.t1 MTR_2g082090 54.324 370 169 0 196 565 210 579 1.10e-144 430
MS.gene33659.t1 MTR_3g056110 49.606 381 191 1 186 565 162 542 2.58e-139 415
MS.gene33659.t1 MTR_5g094450 50.777 386 185 3 185 565 147 532 4.34e-136 406
MS.gene33659.t1 MTR_4g133660 47.568 370 193 1 196 564 173 542 3.43e-126 381
MS.gene33659.t1 MTR_2g026250 47.170 371 195 1 196 565 226 596 5.28e-122 372
MS.gene33659.t1 MTR_7g057230 38.767 454 255 10 119 564 218 656 1.33e-86 282
MS.gene33659.t1 MTR_6g047750 40.409 391 191 10 196 565 293 662 2.33e-81 268
MS.gene33659.t1 MTR_7g027190 37.726 387 211 5 197 565 299 673 2.26e-77 258
MS.gene33659.t1 MTR_3g065980 34.987 383 218 8 199 565 173 540 9.57e-67 226
MS.gene33659.t1 MTR_8g442410 34.908 381 223 7 199 565 156 525 2.92e-64 219
MS.gene33659.t1 MTR_4g064160 31.892 370 251 1 196 564 314 683 2.13e-62 218
MS.gene33659.t1 MTR_7g074650 36.339 366 218 6 201 565 441 792 3.28e-61 216
MS.gene33659.t1 MTR_4g104020 32.723 382 235 5 197 565 148 520 2.16e-60 209
MS.gene33659.t1 MTR_7g062120 30.479 397 269 2 176 565 344 740 1.12e-58 208
MS.gene33659.t1 MTR_4g064200 31.436 369 248 4 198 564 284 649 1.54e-56 201
MS.gene33659.t1 MTR_2g097310 30.376 372 252 3 196 564 270 637 2.23e-55 197
MS.gene33659.t1 MTR_2g097350 31.818 374 244 6 196 564 272 639 2.37e-55 197
MS.gene33659.t1 MTR_4g097080 30.423 378 250 6 196 565 131 503 4.95e-55 194
MS.gene33659.t1 MTR_2g097390 30.108 372 253 3 196 564 273 640 6.64e-55 196
MS.gene33659.t1 MTR_4g064150 29.144 374 260 3 196 564 359 732 6.73e-55 198
MS.gene33659.t1 MTR_4g064120 28.054 442 303 5 130 562 188 623 1.88e-54 195
MS.gene33659.t1 MTR_4g102790 35.602 382 219 14 198 564 292 661 4.47e-54 194
MS.gene33659.t1 MTR_5g009080 28.605 430 240 7 197 565 55 478 4.69e-54 191
MS.gene33659.t1 MTR_2g097410 30.585 376 250 7 196 564 369 740 5.90e-54 195
MS.gene33659.t1 MTR_4g064180 28.217 443 303 5 130 563 188 624 6.29e-54 193
MS.gene33659.t1 MTR_3g022830 27.791 421 248 4 195 565 22 436 1.18e-51 183
MS.gene33659.t1 MTR_4g077760 32.181 376 232 8 199 565 194 555 1.90e-50 182
MS.gene33659.t1 MTR_5g015490 31.392 395 250 10 185 565 103 490 5.60e-50 180
MS.gene33659.t1 MTR_2g097467 28.125 384 270 4 182 564 275 653 3.22e-49 181
MS.gene33659.t1 MTR_4g076020 33.423 371 213 11 201 565 87 429 4.09e-49 176
MS.gene33659.t1 MTR_2g097463 28.533 368 259 3 198 564 290 654 9.33e-48 177
MS.gene33659.t1 MTR_2g097473 26.882 465 312 9 117 564 200 653 2.67e-47 175
MS.gene33659.t1 MTR_3g072710 31.204 407 241 12 185 564 105 499 1.15e-46 171
MS.gene33659.t1 MTR_1g096030 29.471 397 236 12 197 565 79 459 6.41e-45 165
MS.gene33659.t1 MTR_1g069725 32.181 376 224 11 198 564 109 462 1.43e-44 164
MS.gene33659.t1 MTR_5g015950 27.962 422 242 9 196 564 33 445 1.85e-42 158
MS.gene33659.t1 MTR_3g053270 27.962 422 242 9 196 564 33 445 1.85e-42 158
MS.gene33659.t1 MTR_2g097380 27.701 361 229 4 205 564 231 560 5.75e-41 156
MS.gene33659.t1 MTR_2g099110 29.049 389 246 8 197 565 73 451 1.15e-40 153
MS.gene33659.t1 MTR_2g089100 26.018 442 279 8 156 564 16 442 9.88e-40 150
MS.gene33659.t1 MTR_4g095500 26.835 436 273 8 174 564 33 467 3.43e-38 147
MS.gene33659.t1 MTR_5g058860 30.287 383 248 10 197 564 118 496 7.88e-38 146
MS.gene33659.t1 MTR_4g076140 39.333 150 90 1 417 565 320 469 3.08e-30 124
MS.gene33659.t1 MTR_4g076140 29.880 251 164 3 171 420 26 265 2.56e-28 118
MS.gene33659.t1 MTR_2g034250 26.098 410 263 11 178 565 194 585 5.51e-30 124
MS.gene33659.t1 MTR_0092s0100 27.957 372 247 8 199 565 373 728 3.66e-28 119
MS.gene33659.t1 MTR_0092s0100 27.957 372 247 8 199 565 376 731 4.03e-28 119
MS.gene33659.t1 MTR_2g034260 27.642 369 220 12 178 522 194 539 1.32e-27 117
MS.gene33659.t1 MTR_2g034280 25.455 385 256 10 199 565 204 575 2.69e-26 113
MS.gene33659.t1 MTR_7g109580 26.649 379 256 10 197 565 188 554 5.32e-26 112
MS.gene33659.t1 MTR_8g093070 27.441 379 241 12 198 564 139 495 2.39e-25 110
MS.gene33659.t1 MTR_4g026485 25.995 377 247 11 199 565 270 624 7.64e-24 106
MS.gene33659.t1 MTR_1g086970 27.875 287 185 9 287 565 206 478 6.84e-20 93.2
MS.gene33659.t1 MTR_8g020840 23.632 402 265 15 190 565 163 548 9.08e-20 93.2
MS.gene33659.t1 MTR_3g022580 30.864 162 106 2 404 565 28 183 5.29e-18 82.8
MS.gene33659.t1 MTR_3g022005 37.634 93 58 0 473 565 33 125 1.96e-13 67.8
MS.gene33659.t1 MTR_8g077940 21.891 402 236 14 199 565 183 541 2.58e-13 73.2
MS.gene33659.t1 MTR_4g074310 29.237 236 121 7 201 431 2 196 1.68e-12 67.8
MS.gene33659.t1 MTR_4g074320 32.558 172 89 6 171 337 39 188 5.62e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33659.t1 AT1G21450 59.496 595 194 16 1 565 16 593 0.0 644
MS.gene33659.t1 AT5G48150 52.911 395 183 2 172 565 98 490 3.72e-146 430
MS.gene33659.t1 AT5G48150 52.911 395 183 2 172 565 98 490 3.72e-146 430
MS.gene33659.t1 AT5G48150 52.911 395 183 2 172 565 98 490 3.72e-146 430
MS.gene33659.t1 AT5G48150 52.911 395 183 2 172 565 98 490 3.72e-146 430
MS.gene33659.t1 AT1G50600 51.948 385 184 1 182 565 142 526 1.82e-141 420
MS.gene33659.t1 AT1G50600 51.948 385 184 1 182 565 213 597 1.58e-140 420
MS.gene33659.t1 AT1G50600 51.948 385 184 1 182 565 217 601 2.08e-140 419
MS.gene33659.t1 AT2G04890 51.892 370 171 1 196 565 51 413 7.41e-135 399
MS.gene33659.t1 AT4G17230 47.027 370 196 0 196 565 156 525 3.61e-124 375
MS.gene33659.t1 AT4G17230 47.027 370 196 0 196 565 156 525 3.61e-124 375
MS.gene33659.t1 AT5G52510 39.481 385 208 5 196 565 266 640 3.83e-85 278
MS.gene33659.t1 AT1G66350 37.366 372 207 9 199 565 156 506 3.55e-69 232
MS.gene33659.t1 AT3G03450 36.340 377 215 9 199 565 184 545 1.67e-67 228
MS.gene33659.t1 AT5G17490 35.242 454 246 13 136 565 87 516 2.24e-67 228
MS.gene33659.t1 AT3G54220 36.856 369 213 8 201 564 296 649 2.35e-65 225
MS.gene33659.t1 AT1G07530 33.760 391 254 3 176 564 377 764 6.02e-64 223
MS.gene33659.t1 AT5G66770 36.340 377 217 9 199 565 221 584 2.57e-60 210
MS.gene33659.t1 AT1G14920 33.780 373 225 10 199 565 173 529 1.92e-59 206
MS.gene33659.t1 AT2G01570 34.301 379 215 12 199 565 225 581 3.30e-58 204
MS.gene33659.t1 AT2G29060 31.980 394 252 5 179 564 269 654 5.24e-57 202
MS.gene33659.t1 AT2G29060 31.980 394 252 5 179 564 306 691 8.88e-57 202
MS.gene33659.t1 AT5G59450 29.777 403 271 5 172 565 199 598 5.36e-56 199
MS.gene33659.t1 AT2G37650 29.650 371 258 2 196 565 333 701 3.01e-55 198
MS.gene33659.t1 AT2G37650 29.650 371 258 2 196 565 345 713 3.29e-55 198
MS.gene33659.t1 AT2G29065 30.423 378 254 5 196 564 250 627 9.28e-54 193
MS.gene33659.t1 AT3G46600 29.919 371 258 2 196 565 2 371 1.63e-53 186
MS.gene33659.t1 AT3G46600 29.919 371 258 2 196 565 210 579 2.70e-52 188
MS.gene33659.t1 AT3G46600 29.919 371 258 2 196 565 178 547 2.75e-52 187
MS.gene33659.t1 AT5G41920 32.718 379 223 10 198 565 43 400 6.80e-51 180
MS.gene33659.t1 AT3G50650 33.983 359 209 7 221 565 198 542 2.18e-50 182
MS.gene33659.t1 AT1G07520 27.820 399 272 5 182 564 130 528 8.44e-50 180
MS.gene33659.t1 AT1G07520 27.820 399 272 5 182 564 130 528 8.44e-50 180
MS.gene33659.t1 AT1G07520 27.820 399 272 5 182 564 294 692 1.25e-49 182
MS.gene33659.t1 AT1G07520 27.820 399 272 5 182 564 295 693 1.36e-49 182
MS.gene33659.t1 AT4G37650 29.206 428 259 11 165 565 119 529 1.35e-48 177
MS.gene33659.t1 AT1G63100 34.347 329 196 7 198 518 252 568 3.24e-43 164
MS.gene33659.t1 AT1G63100 33.141 347 212 7 198 536 277 611 3.83e-43 164
MS.gene33659.t1 AT4G08250 29.532 342 221 7 233 564 145 476 9.59e-41 154
MS.gene33659.t1 AT1G55580 27.119 413 249 9 196 565 42 445 6.92e-40 151
MS.gene33659.t1 AT3G49950 27.979 386 255 10 196 564 28 407 1.65e-38 146
MS.gene33659.t1 AT1G50420 32.857 210 132 2 197 403 56 259 9.41e-31 125
MS.gene33659.t1 AT1G50420 37.748 151 91 2 417 565 330 479 9.72e-28 117
MS.gene33659.t1 AT3G13840 26.000 400 267 11 178 565 128 510 4.54e-22 100
MS.gene33659.t1 AT3G60630 25.501 349 229 10 231 565 291 622 7.83e-21 97.1
MS.gene33659.t1 AT2G45160 26.214 309 202 8 268 565 346 639 1.47e-20 96.3
MS.gene33659.t1 AT4G00150 24.069 349 234 10 222 565 232 554 2.63e-18 89.0
MS.gene33659.t1 AT5G67411 34.171 199 118 7 315 502 2 198 1.12e-17 82.0
MS.gene33659.t1 AT4G36710 23.864 352 244 9 222 565 150 485 8.85e-16 80.5

Find 115 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTATGAGAATAAGAAATT+TGG 0.178504 3.4:-82345457 None:intergenic
GGTACAACCATGTCATTAAT+TGG 0.248675 3.4:+82345350 None:intergenic
CCATGTAGGCTGCAATTCTT+TGG 0.255636 3.4:-82346054 None:intergenic
GAAAAGGGTGCACTTCATTT+TGG 0.257256 3.4:+82346991 MS.gene33659:CDS
CACCAAATCTGATCCGAAAT+TGG 0.280682 3.4:-82345627 None:intergenic
GGTATGAAGTCGCGGGAAAA+TGG 0.287860 3.4:+82346857 MS.gene33659:CDS
GCTACTGATGCTGCTAATAT+TGG 0.307471 3.4:-82345885 None:intergenic
TCCACGACCGTTACAAGTTT+TGG 0.308347 3.4:-82346640 None:intergenic
AAGAAATAACGAAAATTTCT+AGG 0.316566 3.4:+82345915 MS.gene33659:CDS
GTCCAATTTCGGATCAGATT+TGG 0.328151 3.4:+82345625 MS.gene33659:CDS
TCGAAGCAAGTGTTTGAATC+AGG 0.332729 3.4:-82346312 None:intergenic
TTGATCCTAACATTATTGAT+TGG 0.340862 3.4:+82345786 MS.gene33659:CDS
CTTATGAGAATAAGAAATTT+GGG 0.359390 3.4:-82345456 None:intergenic
TCAGCACCACGTTGCCTTGT+TGG 0.374411 3.4:-82345599 None:intergenic
TCGATGCTTGATTGTCGAAT+TGG 0.376588 3.4:+82346508 MS.gene33659:CDS
GGTGCTGATCAGTCCAATTT+CGG 0.378562 3.4:+82345614 MS.gene33659:CDS
TCACTCACATTTGTGCTCAT+TGG 0.383131 3.4:-82346916 None:intergenic
GTACAAGATAAAGGAGGAAA+AGG 0.383813 3.4:+82346975 MS.gene33659:CDS
GCCGCTCGCCTCGCTGAATC+CGG 0.387906 3.4:+82346088 MS.gene33659:CDS
ATTGAGGAAGAAGAGGAATA+TGG 0.391911 3.4:+82345743 MS.gene33659:CDS
TGATCCTAACATTATTGATT+GGG 0.392056 3.4:+82345787 MS.gene33659:CDS
CGACGAATTGTCTCAATTCA+AGG 0.402603 3.4:+82346022 MS.gene33659:CDS
GGCAAGGGATATTGTTAATA+CGG 0.403210 3.4:+82346804 MS.gene33659:CDS
CATTCATAAAGAAGTTGCTT+GGG 0.414263 3.4:-82345941 None:intergenic
AACAGCAGCATATTCTGAAT+TGG 0.421315 3.4:-82345821 None:intergenic
CTGTGATAGGTACAAGATAA+AGG 0.425096 3.4:+82346966 MS.gene33659:CDS
GGGTTAACATCGATTTCCTT+TGG 0.428305 3.4:-82345422 None:intergenic
GTGAATTCAATTGAAGTTGA+TGG 0.432801 3.4:+82345665 MS.gene33659:CDS
TCAGCCCAATCAATAATGTT+AGG 0.434921 3.4:-82345791 None:intergenic
CCATCACATGTTAGATTGAC+CGG 0.440788 3.4:+82346346 MS.gene33659:CDS
ATGCTGCTAATATTGGAATC+TGG 0.446519 3.4:-82345878 None:intergenic
CATGTAGGCTGCAATTCTTT+GGG 0.448246 3.4:-82346053 None:intergenic
ATGCAAGGAACCTCCTTCTT+CGG 0.450058 3.4:+82346135 MS.gene33659:CDS
AAGTTAAGTTGTACTGTGAT+AGG 0.450787 3.4:+82346953 MS.gene33659:CDS
CCAAAGAATTGCAGCCTACA+TGG 0.451697 3.4:+82346054 MS.gene33659:CDS
GAGATCAAAACTAGAAGCAT+TGG 0.459866 3.4:+82345694 MS.gene33659:CDS
ACAGTTGAAACGCTCTCGTC+AGG 0.461394 3.4:-82346574 None:intergenic
TCTGGAGACGAATACTCCTT+TGG 0.473491 3.4:-82345860 None:intergenic
TTCGGATCAGATTTGGTGAT+GGG 0.474390 3.4:+82345632 MS.gene33659:CDS
TGATTCAAACACTTGCTTCG+AGG 0.476019 3.4:+82346314 MS.gene33659:CDS
TACTCTTCCTAGAGAGAGCC+AGG 0.477900 3.4:+82346753 MS.gene33659:CDS
AGCATATTCTGAATTGGATC+AGG 0.480387 3.4:-82345815 None:intergenic
CGTCAGGCATGTGATGAAGC+TGG 0.484030 3.4:-82346558 None:intergenic
TTGCATGTGAAGGTGAAGAT+AGG 0.487421 3.4:+82346827 MS.gene33659:CDS
TCTCAACATTCATCCTATCC+TGG 0.491515 3.4:-82346771 None:intergenic
GTTGGAGGACTAAAGATCAT+AGG 0.492091 3.4:+82346400 MS.gene33659:CDS
AGCGATGCAGATACTGTTCG+AGG 0.503528 3.4:+82346168 MS.gene33659:CDS
ACGAGACCAACTTCTTCGCT+TGG 0.503960 3.4:+82346603 MS.gene33659:CDS
ACATTCATAAAGAAGTTGCT+TGG 0.509000 3.4:-82345942 None:intergenic
ATCCTATCCTGGCTCTCTCT+AGG 0.512100 3.4:-82346760 None:intergenic
TCTTTACCAAGCGAAGAAGT+TGG 0.520115 3.4:-82346609 None:intergenic
TCGCGGGAAAATGGAGAGCT+AGG 0.521293 3.4:+82346866 MS.gene33659:CDS
GCTGCTAGACGGTCCGAAGA+AGG 0.532544 3.4:-82346148 None:intergenic
TCTGTGCAGCGATCCGTTGG+AGG 0.533052 3.4:+82346385 MS.gene33659:CDS
CGAAGCAAGTGTTTGAATCA+GGG 0.537450 3.4:-82346311 None:intergenic
GTAGAGATGTCAGAACTAGA+TGG 0.537559 3.4:-82345566 None:intergenic
GAGTCTGTGCAGCGATCCGT+TGG 0.538684 3.4:+82346382 MS.gene33659:CDS
TCCAAAACTTGTAACGGTCG+TGG 0.541218 3.4:+82346639 MS.gene33659:CDS
TTATAGAGCTTTGAAATGCA+AGG 0.546542 3.4:+82346120 MS.gene33659:CDS
TACAAGATAAAGGAGGAAAA+GGG 0.547340 3.4:+82346976 MS.gene33659:CDS
AGAGCTAGGATGACAATGGC+TGG 0.548519 3.4:+82346880 MS.gene33659:CDS
TTTGAATCCAAAACTTGTAA+CGG 0.552516 3.4:+82346633 MS.gene33659:CDS
ACTTGATTGTTGCTTCAGCA+TGG 0.554425 3.4:+82347028 MS.gene33659:CDS
TAGTGACATTGAGGAAGAAG+AGG 0.556415 3.4:+82345736 MS.gene33659:CDS
ACGGATCGCTGCACAGACTC+AGG 0.557758 3.4:-82346379 None:intergenic
GAACAACTAGCAGAAACACT+TGG 0.558635 3.4:+82346433 MS.gene33659:CDS
ACTTGATGATAGTGACATTG+AGG 0.558721 3.4:+82345727 MS.gene33659:CDS
TTTCGGATCAGATTTGGTGA+TGG 0.559466 3.4:+82345631 MS.gene33659:CDS
ATATGGTAATTCTCAAAGCA+TGG 0.562265 3.4:+82345760 MS.gene33659:CDS
CCGGTCAATCTAACATGTGA+TGG 0.566500 3.4:-82346346 None:intergenic
CATCACATGTTAGATTGACC+GGG 0.567991 3.4:+82346347 MS.gene33659:CDS
TTGAGAGGCAATGCTTGGCA+AGG 0.577561 3.4:+82346788 MS.gene33659:CDS
AATTGCAGCCTACATGGTGG+AGG 0.577669 3.4:+82346060 MS.gene33659:CDS
TAGGCTGCAATTCTTTGGGA+AGG 0.583102 3.4:-82346049 None:intergenic
CAAAACTAGAAGCATTGGAG+AGG 0.583413 3.4:+82345699 MS.gene33659:CDS
GAATGTTGAGAGGCAATGCT+TGG 0.583862 3.4:+82346783 MS.gene33659:CDS
CTACAAGTTTCATGCCAACA+AGG 0.584293 3.4:+82345585 MS.gene33659:CDS
TGTGCTACTGCAATTTCAGA+AGG 0.584377 3.4:+82345962 MS.gene33659:CDS
TCCGGATTCAGCGAGGCGAG+CGG 0.592435 3.4:-82346089 None:intergenic
AATACTCCTTTGGTGAGTCA+TGG 0.604923 3.4:-82345850 None:intergenic
CACTAAGCTCAAATCGCCAA+AGG 0.608012 3.4:+82345406 MS.gene33659:CDS
TATGATCTTTAGTCCTCCAA+CGG 0.609253 3.4:-82346398 None:intergenic
GTATCTGCATCGCTGCTAGA+CGG 0.609923 3.4:-82346159 None:intergenic
CTCATAACTCTCGCTCGAGT+AGG 0.609952 3.4:-82345504 None:intergenic
ATTCATAAAGAAGTTGCTTG+GGG 0.610084 3.4:-82345940 None:intergenic
GTTAATACGGTTGCATGTGA+AGG 0.612068 3.4:+82346817 MS.gene33659:CDS
ATAGAACGGTATGAAGTCGC+GGG 0.612894 3.4:+82346850 MS.gene33659:CDS
AGACTCAGGATCATCAATCC+CGG 0.614792 3.4:-82346365 None:intergenic
TAACTATGCAATAACAGAAG+CGG 0.616342 3.4:+82346225 MS.gene33659:CDS
GATAGAACGGTATGAAGTCG+CGG 0.616637 3.4:+82346849 MS.gene33659:CDS
CCTTTGGTGAGTCATGGTCA+TGG 0.621918 3.4:-82345844 None:intergenic
AAGGTGAAGATAGGATAGAA+CGG 0.625100 3.4:+82346836 MS.gene33659:CDS
TTCCTAGAGAGAGCCAGGAT+AGG 0.625909 3.4:+82346758 MS.gene33659:CDS
TCGAATTGGAGAAGCTCTTG+TGG 0.628740 3.4:+82346522 MS.gene33659:CDS
TGAGAGGCAATGCTTGGCAA+GGG 0.635494 3.4:+82346789 MS.gene33659:CDS
AAAACTAGAAGCATTGGAGA+GGG 0.649054 3.4:+82345700 MS.gene33659:CDS
AGAAGATCCAATTAATGACA+TGG 0.650375 3.4:-82345357 None:intergenic
ATTGCAGCCTACATGGTGGA+GGG 0.650477 3.4:+82346061 MS.gene33659:CDS
GGCGAGTCCCTCCACCATGT+AGG 0.652264 3.4:-82346068 None:intergenic
GCTAGACGGTCCGAAGAAGG+AGG 0.655079 3.4:-82346145 None:intergenic
TTCATAAAGAAGTTGCTTGG+GGG 0.656482 3.4:-82345939 None:intergenic
ATGAGAATAAGAAATTTGGG+AGG 0.658872 3.4:-82345453 None:intergenic
AGGATAGGATGAATGTTGAG+AGG 0.660140 3.4:+82346773 MS.gene33659:CDS
AATTTCAGAAGGAAATGAAG+AGG 0.662867 3.4:+82345973 MS.gene33659:CDS
ATGGAGAGCTAGGATGACAA+TGG 0.667953 3.4:+82346876 MS.gene33659:CDS
CCATGACCATGACTCACCAA+AGG 0.671972 3.4:+82345844 MS.gene33659:CDS
CTTGAATTGAGACAATTCGT+CGG 0.672967 3.4:-82346021 None:intergenic
AATTTCGAGCGGTCTCATCG+AGG 0.679065 3.4:+82346467 MS.gene33659:CDS
TTTGTCATTTGAATTTCGAG+CGG 0.680630 3.4:+82346456 MS.gene33659:CDS
AAGAATTGCAGCCTACATGG+TGG 0.682524 3.4:+82346057 MS.gene33659:CDS
TCGACAATCAAGCATCGATG+AGG 0.688280 3.4:-82346503 None:intergenic
TGATAGGTACAAGATAAAGG+AGG 0.692576 3.4:+82346969 MS.gene33659:CDS
ATAAACCCGAATTTAAAACA+CGG 0.716869 3.4:-82346196 None:intergenic
TTCATGCCAACAAGGCAACG+TGG 0.722675 3.4:+82345593 MS.gene33659:CDS
TGATTGTTGCTTCAGCATGG+AGG 0.748962 3.4:+82347031 MS.gene33659:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAACGAAAATTTCT+AGG + chr3.4:82345915-82345934 MS.gene33659:CDS 20.0%
!! CTTATGAGAATAAGAAATTT+GGG - chr3.4:82345459-82345478 None:intergenic 20.0%
!!! AGAAATTTTCGTTATTTCTT+TGG - chr3.4:82345915-82345934 None:intergenic 20.0%
!!! ATAGATTTTGATATCAATCA+AGG + chr3.4:82346274-82346293 MS.gene33659:CDS 20.0%
! GCTTATGAGAATAAGAAATT+TGG - chr3.4:82345460-82345479 None:intergenic 25.0%
! TTGATCCTAACATTATTGAT+TGG + chr3.4:82345786-82345805 MS.gene33659:CDS 25.0%
! TTTGAATCCAAAACTTGTAA+CGG + chr3.4:82346633-82346652 MS.gene33659:CDS 25.0%
!! ATAAACCCGAATTTAAAACA+CGG - chr3.4:82346199-82346218 None:intergenic 25.0%
!! TGATCCTAACATTATTGATT+GGG + chr3.4:82345787-82345806 MS.gene33659:CDS 25.0%
!!! AATTTTCGTTATTTCTTTGG+TGG - chr3.4:82345912-82345931 None:intergenic 25.0%
!!! AGCTCTATAAATACTTTTTC+CGG - chr3.4:82346110-82346129 None:intergenic 25.0%
!!! AGTACAACTTAACTTGTTTT+CGG - chr3.4:82346948-82346967 None:intergenic 25.0%
AAATTTGGGAGGAAAAATCA+GGG - chr3.4:82345445-82345464 None:intergenic 30.0%
AATTTCAGAAGGAAATGAAG+AGG + chr3.4:82345973-82345992 MS.gene33659:CDS 30.0%
ACATTCATAAAGAAGTTGCT+TGG - chr3.4:82345945-82345964 None:intergenic 30.0%
ATATGGTAATTCTCAAAGCA+TGG + chr3.4:82345760-82345779 MS.gene33659:CDS 30.0%
ATGAGAATAAGAAATTTGGG+AGG - chr3.4:82345456-82345475 None:intergenic 30.0%
ATTCATAAAGAAGTTGCTTG+GGG - chr3.4:82345943-82345962 None:intergenic 30.0%
CATTCATAAAGAAGTTGCTT+GGG - chr3.4:82345944-82345963 None:intergenic 30.0%
GTGAATTCAATTGAAGTTGA+TGG + chr3.4:82345665-82345684 MS.gene33659:CDS 30.0%
TAACTATGCAATAACAGAAG+CGG + chr3.4:82346225-82346244 MS.gene33659:CDS 30.0%
TACAAGATAAAGGAGGAAAA+GGG + chr3.4:82346976-82346995 MS.gene33659:CDS 30.0%
TCAACAAATCTCTGCAAAAA+CGG - chr3.4:82346691-82346710 None:intergenic 30.0%
! AAGTTAAGTTGTACTGTGAT+AGG + chr3.4:82346953-82346972 MS.gene33659:CDS 30.0%
! TTTGTCATTTGAATTTCGAG+CGG + chr3.4:82346456-82346475 MS.gene33659:CDS 30.0%
!! GTTTGTCCGTGTTTTAAATT+CGG + chr3.4:82346190-82346209 MS.gene33659:CDS 30.0%
!! TTATAGAGCTTTGAAATGCA+AGG + chr3.4:82346120-82346139 MS.gene33659:CDS 30.0%
!! TTTGTCCGTGTTTTAAATTC+GGG + chr3.4:82346191-82346210 MS.gene33659:CDS 30.0%
AACAAATCTCTGCAAAAACG+GGG - chr3.4:82346689-82346708 None:intergenic 35.0%
AACAGCAGCATATTCTGAAT+TGG - chr3.4:82345824-82345843 None:intergenic 35.0%
AAGGTGAAGATAGGATAGAA+CGG + chr3.4:82346836-82346855 MS.gene33659:CDS 35.0%
AGCATATTCTGAATTGGATC+AGG - chr3.4:82345818-82345837 None:intergenic 35.0%
ATGCTGCTAATATTGGAATC+TGG - chr3.4:82345881-82345900 None:intergenic 35.0%
ATTGAGGAAGAAGAGGAATA+TGG + chr3.4:82345743-82345762 MS.gene33659:CDS 35.0%
CAACAAATCTCTGCAAAAAC+GGG - chr3.4:82346690-82346709 None:intergenic 35.0%
CTGTGATAGGTACAAGATAA+AGG + chr3.4:82346966-82346985 MS.gene33659:CDS 35.0%
CTTGAATTGAGACAATTCGT+CGG - chr3.4:82346024-82346043 None:intergenic 35.0%
GAAATTTGGGAGGAAAAATC+AGG - chr3.4:82345446-82345465 None:intergenic 35.0%
GGCAAGGGATATTGTTAATA+CGG + chr3.4:82346804-82346823 MS.gene33659:CDS 35.0%
GTACAAGATAAAGGAGGAAA+AGG + chr3.4:82346975-82346994 MS.gene33659:CDS 35.0%
TATGATCTTTAGTCCTCCAA+CGG - chr3.4:82346401-82346420 None:intergenic 35.0%
TCAGCCCAATCAATAATGTT+AGG - chr3.4:82345794-82345813 None:intergenic 35.0%
TGATAGGTACAAGATAAAGG+AGG + chr3.4:82346969-82346988 MS.gene33659:CDS 35.0%
TTCATAAAGAAGTTGCTTGG+GGG - chr3.4:82345942-82345961 None:intergenic 35.0%
! ACTTGATGATAGTGACATTG+AGG + chr3.4:82345727-82345746 MS.gene33659:CDS 35.0%
! AGCTTAGTGCTTTTATGTGA+TGG - chr3.4:82345395-82345414 None:intergenic 35.0%
! GAGATCAAAACTAGAAGCAT+TGG + chr3.4:82345694-82345713 MS.gene33659:CDS 35.0%
! TTAGTGCTTTTATGTGATGG+TGG - chr3.4:82345392-82345411 None:intergenic 35.0%
!! AAAACTAGAAGCATTGGAGA+GGG + chr3.4:82345700-82345719 MS.gene33659:CDS 35.0%
AATACTCCTTTGGTGAGTCA+TGG - chr3.4:82345853-82345872 None:intergenic 40.0%
AGGATAGGATGAATGTTGAG+AGG + chr3.4:82346773-82346792 MS.gene33659:CDS 40.0%
CACCAAATCTGATCCGAAAT+TGG - chr3.4:82345630-82345649 None:intergenic 40.0%
CATCACATGTTAGATTGACC+GGG + chr3.4:82346347-82346366 MS.gene33659:CDS 40.0%
CATGTAGGCTGCAATTCTTT+GGG - chr3.4:82346056-82346075 None:intergenic 40.0%
CCATCACATGTTAGATTGAC+CGG + chr3.4:82346346-82346365 MS.gene33659:CDS 40.0%
CGAAGCAAGTGTTTGAATCA+GGG - chr3.4:82346314-82346333 None:intergenic 40.0%
CGACGAATTGTCTCAATTCA+AGG + chr3.4:82346022-82346041 MS.gene33659:CDS 40.0%
CTACAAGTTTCATGCCAACA+AGG + chr3.4:82345585-82345604 MS.gene33659:CDS 40.0%
GAACAACTAGCAGAAACACT+TGG + chr3.4:82346433-82346452 MS.gene33659:CDS 40.0%
GCTACTGATGCTGCTAATAT+TGG - chr3.4:82345888-82345907 None:intergenic 40.0%
GTAGAGATGTCAGAACTAGA+TGG - chr3.4:82345569-82345588 None:intergenic 40.0%
GTCCAATTTCGGATCAGATT+TGG + chr3.4:82345625-82345644 MS.gene33659:CDS 40.0%
GTTAATACGGTTGCATGTGA+AGG + chr3.4:82346817-82346836 MS.gene33659:CDS 40.0%
GTTGGAGGACTAAAGATCAT+AGG + chr3.4:82346400-82346419 MS.gene33659:CDS 40.0%
TAGTGACATTGAGGAAGAAG+AGG + chr3.4:82345736-82345755 MS.gene33659:CDS 40.0%
TCACTCACATTTGTGCTCAT+TGG - chr3.4:82346919-82346938 None:intergenic 40.0%
TCGAAGCAAGTGTTTGAATC+AGG - chr3.4:82346315-82346334 None:intergenic 40.0%
TCGATGCTTGATTGTCGAAT+TGG + chr3.4:82346508-82346527 MS.gene33659:CDS 40.0%
TCTCAACATTCATCCTATCC+TGG - chr3.4:82346774-82346793 None:intergenic 40.0%
TCTTTACCAAGCGAAGAAGT+TGG - chr3.4:82346612-82346631 None:intergenic 40.0%
TGATTCAAACACTTGCTTCG+AGG + chr3.4:82346314-82346333 MS.gene33659:CDS 40.0%
TTGCATGTGAAGGTGAAGAT+AGG + chr3.4:82346827-82346846 MS.gene33659:CDS 40.0%
! ACTTGATTGTTGCTTCAGCA+TGG + chr3.4:82347028-82347047 MS.gene33659:CDS 40.0%
! TGTGCTACTGCAATTTCAGA+AGG + chr3.4:82345962-82345981 MS.gene33659:CDS 40.0%
!! CAAAACTAGAAGCATTGGAG+AGG + chr3.4:82345699-82345718 MS.gene33659:CDS 40.0%
!! GAAAAGGGTGCACTTCATTT+TGG + chr3.4:82346991-82347010 MS.gene33659:CDS 40.0%
!! GGGTTAACATCGATTTCCTT+TGG - chr3.4:82345425-82345444 None:intergenic 40.0%
!! TTCGGATCAGATTTGGTGAT+GGG + chr3.4:82345632-82345651 MS.gene33659:CDS 40.0%
!! TTTCGGATCAGATTTGGTGA+TGG + chr3.4:82345631-82345650 MS.gene33659:CDS 40.0%
AAATCTCTGCAAAAACGGGG+CGG - chr3.4:82346686-82346705 None:intergenic 45.0%
AAGAATTGCAGCCTACATGG+TGG + chr3.4:82346057-82346076 MS.gene33659:CDS 45.0%
AGACTCAGGATCATCAATCC+CGG - chr3.4:82346368-82346387 None:intergenic 45.0%
ATAGAACGGTATGAAGTCGC+GGG + chr3.4:82346850-82346869 MS.gene33659:CDS 45.0%
ATGCAAGGAACCTCCTTCTT+CGG + chr3.4:82346135-82346154 MS.gene33659:CDS 45.0%
ATGGAGAGCTAGGATGACAA+TGG + chr3.4:82346876-82346895 MS.gene33659:CDS 45.0%
CACTAAGCTCAAATCGCCAA+AGG + chr3.4:82345406-82345425 MS.gene33659:CDS 45.0%
CCAAAGAATTGCAGCCTACA+TGG + chr3.4:82346054-82346073 MS.gene33659:CDS 45.0%
CCATGTAGGCTGCAATTCTT+TGG - chr3.4:82346057-82346076 None:intergenic 45.0%
CCGGTCAATCTAACATGTGA+TGG - chr3.4:82346349-82346368 None:intergenic 45.0%
GAATGTTGAGAGGCAATGCT+TGG + chr3.4:82346783-82346802 MS.gene33659:CDS 45.0%
GATAGAACGGTATGAAGTCG+CGG + chr3.4:82346849-82346868 MS.gene33659:CDS 45.0%
TCCAAAACTTGTAACGGTCG+TGG + chr3.4:82346639-82346658 MS.gene33659:CDS 45.0%
TCGAATTGGAGAAGCTCTTG+TGG + chr3.4:82346522-82346541 MS.gene33659:CDS 45.0%
TCTGGAGACGAATACTCCTT+TGG - chr3.4:82345863-82345882 None:intergenic 45.0%
! TAGGCTGCAATTCTTTGGGA+AGG - chr3.4:82346052-82346071 None:intergenic 45.0%
! TCCACGACCGTTACAAGTTT+TGG - chr3.4:82346643-82346662 None:intergenic 45.0%
! TGATTGTTGCTTCAGCATGG+AGG + chr3.4:82347031-82347050 MS.gene33659:CDS 45.0%
!! AGGGTGCACTTCATTTTGGT+TGG + chr3.4:82346995-82347014 MS.gene33659:CDS 45.0%
!! GGTGCTGATCAGTCCAATTT+CGG + chr3.4:82345614-82345633 MS.gene33659:CDS 45.0%
!! TCGACAATCAAGCATCGATG+AGG - chr3.4:82346506-82346525 None:intergenic 45.0%
!!! GGGTGCACTTCATTTTGGTT+GGG + chr3.4:82346996-82347015 MS.gene33659:CDS 45.0%
!!! TACTTTTTCCGGATTCAGCG+AGG - chr3.4:82346099-82346118 None:intergenic 45.0%
AATTGCAGCCTACATGGTGG+AGG + chr3.4:82346060-82346079 MS.gene33659:CDS 50.0%
AATTTCGAGCGGTCTCATCG+AGG + chr3.4:82346467-82346486 MS.gene33659:CDS 50.0%
ACAGTTGAAACGCTCTCGTC+AGG - chr3.4:82346577-82346596 None:intergenic 50.0%
ACGAGACCAACTTCTTCGCT+TGG + chr3.4:82346603-82346622 MS.gene33659:CDS 50.0%
AGAGCTAGGATGACAATGGC+TGG + chr3.4:82346880-82346899 MS.gene33659:CDS 50.0%
AGCGATGCAGATACTGTTCG+AGG + chr3.4:82346168-82346187 MS.gene33659:CDS 50.0%
CCATGACCATGACTCACCAA+AGG + chr3.4:82345844-82345863 MS.gene33659:CDS 50.0%
CCTTTGGTGAGTCATGGTCA+TGG - chr3.4:82345847-82345866 None:intergenic 50.0%
CTCATAACTCTCGCTCGAGT+AGG - chr3.4:82345507-82345526 None:intergenic 50.0%
GGTATGAAGTCGCGGGAAAA+TGG + chr3.4:82346857-82346876 MS.gene33659:CDS 50.0%
GTATCTGCATCGCTGCTAGA+CGG - chr3.4:82346162-82346181 None:intergenic 50.0%
TACTCTTCCTAGAGAGAGCC+AGG + chr3.4:82346753-82346772 MS.gene33659:CDS 50.0%
TGAGAGGCAATGCTTGGCAA+GGG + chr3.4:82346789-82346808 MS.gene33659:CDS 50.0%
TTCATGCCAACAAGGCAACG+TGG + chr3.4:82345593-82345612 MS.gene33659:CDS 50.0%
TTCCTAGAGAGAGCCAGGAT+AGG + chr3.4:82346758-82346777 MS.gene33659:CDS 50.0%
TTGAGAGGCAATGCTTGGCA+AGG + chr3.4:82346788-82346807 MS.gene33659:CDS 50.0%
! ATCCTATCCTGGCTCTCTCT+AGG - chr3.4:82346763-82346782 None:intergenic 50.0%
! ATTGCAGCCTACATGGTGGA+GGG + chr3.4:82346061-82346080 MS.gene33659:CDS 50.0%
CGTCAGGCATGTGATGAAGC+TGG - chr3.4:82346561-82346580 None:intergenic 55.0%
CTGCAAAAACGGGGCGGTAT+TGG - chr3.4:82346680-82346699 None:intergenic 55.0%
TCAGCACCACGTTGCCTTGT+TGG - chr3.4:82345602-82345621 None:intergenic 55.0%
TCGCGGGAAAATGGAGAGCT+AGG + chr3.4:82346866-82346885 MS.gene33659:CDS 55.0%
ACGGATCGCTGCACAGACTC+AGG - chr3.4:82346382-82346401 None:intergenic 60.0%
GAGTCTGTGCAGCGATCCGT+TGG + chr3.4:82346382-82346401 MS.gene33659:CDS 60.0%
GCTAGACGGTCCGAAGAAGG+AGG - chr3.4:82346148-82346167 None:intergenic 60.0%
GCTGCTAGACGGTCCGAAGA+AGG - chr3.4:82346151-82346170 None:intergenic 60.0%
TCTGTGCAGCGATCCGTTGG+AGG + chr3.4:82346385-82346404 MS.gene33659:CDS 60.0%
GGCGAGTCCCTCCACCATGT+AGG - chr3.4:82346071-82346090 None:intergenic 65.0%
TCCGGATTCAGCGAGGCGAG+CGG - chr3.4:82346092-82346111 None:intergenic 65.0%
GCCGCTCGCCTCGCTGAATC+CGG + chr3.4:82346088-82346107 MS.gene33659:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 82345359 82347056 82345359 ID=MS.gene33659
chr3.4 mRNA 82345359 82347056 82345359 ID=MS.gene33659.t1;Parent=MS.gene33659
chr3.4 exon 82345359 82347056 82345359 ID=MS.gene33659.t1.exon1;Parent=MS.gene33659.t1
chr3.4 CDS 82345359 82347056 82345359 ID=cds.MS.gene33659.t1;Parent=MS.gene33659.t1
Gene Sequence

>MS.gene33659

ATGTCATTAATTGGATCTTCTGAGCTAGCTCCACCATCACATAAAAGCACTAAGCTCAAATCGCCAAAGGAAATCGATGTTAACCCTGATTTTTCCTCCCAAATTTCTTATTCTCATAAGCATAGAAGCATGTATATCACAGATACCTACTCGAGCGAGAGTTATGAGAAGTACTTCAATGACTCACAGACAGAAGAACTAATAGAACCATCTAGTTCTGACATCTCTACAAGTTTCATGCCAACAAGGCAACGTGGTGCTGATCAGTCCAATTTCGGATCAGATTTGGTGATGGGAAATCGAAGCGTGAATTCAATTGAAGTTGATGGTGCAATGAGATCAAAACTAGAAGCATTGGAGAGGGCATTACTTGATGATAGTGACATTGAGGAAGAAGAGGAATATGGTAATTCTCAAAGCATGGAAATTGATCCTAACATTATTGATTGGGCTGATCCTGATCCAATTCAGAATATGCTGCTGTTCCATGACCATGACTCACCAAAGGAGTATTCGTCTCCAGATTCCAATATTAGCAGCATCAGTAGCACCACCAAAGAAATAACGAAAATTTCTAGGACCCCCAAGCAACTTCTTTATGAATGTGCTACTGCAATTTCAGAAGGAAATGAAGAGGAAGCATCGTCGATGATAAACAATCTCCGACGAATTGTCTCAATTCAAGGAGAGCCTTCCCAAAGAATTGCAGCCTACATGGTGGAGGGACTCGCCGCTCGCCTCGCTGAATCCGGAAAAAGTATTTATAGAGCTTTGAAATGCAAGGAACCTCCTTCTTCGGACCGTCTAGCAGCGATGCAGATACTGTTCGAGGTTTGTCCGTGTTTTAAATTCGGGTTTATTGCAGCTAACTATGCAATAACAGAAGCGGTTAAAGATGAAATGAAGATTCATATAATAGATTTTGATATCAATCAAGGAAGTCAGTACATAACCCTGATTCAAACACTTGCTTCGAGGTCTTGTAAGCCATCACATGTTAGATTGACCGGGATTGATGATCCTGAGTCTGTGCAGCGATCCGTTGGAGGACTAAAGATCATAGGACAAAGACTTGAACAACTAGCAGAAACACTTGGTTTGTCATTTGAATTTCGAGCGGTCTCATCGAGGACTTCAATTGTCACCTCATCGATGCTTGATTGTCGAATTGGAGAAGCTCTTGTGGTGAACTTTGCATTCCAGCTTCATCACATGCCTGACGAGAGCGTTTCAACTGTGAACGAACGAGACCAACTTCTTCGCTTGGTAAAGAGTTTGAATCCAAAACTTGTAACGGTCGTGGAGCAAGATGTAAACACCAATACCGCCCCGTTTTTGCAGAGATTTGTTGAAGCTTACAATTACTACTCTGCTGTGTTTGAGTCACTTGATGTTACTCTTCCTAGAGAGAGCCAGGATAGGATGAATGTTGAGAGGCAATGCTTGGCAAGGGATATTGTTAATACGGTTGCATGTGAAGGTGAAGATAGGATAGAACGGTATGAAGTCGCGGGAAAATGGAGAGCTAGGATGACAATGGCTGGATTCAGTTCATCACCAATGAGCACAAATGTGAGTGATGAAATCCGAAAACAAGTTAAGTTGTACTGTGATAGGTACAAGATAAAGGAGGAAAAGGGTGCACTTCATTTTGGTTGGGAAGACAAAAACTTGATTGTTGCTTCAGCATGGAGGTGA
Protein sequence

>MS.gene33659.t1

MSLIGSSELAPPSHKSTKLKSPKEIDVNPDFSSQISYSHKHRSMYITDTYSSESYEKYFNDSQTEELIEPSSSDISTSFMPTRQRGADQSNFGSDLVMGNRSVNSIEVDGAMRSKLEALERALLDDSDIEEEEEYGNSQSMEIDPNIIDWADPDPIQNMLLFHDHDSPKEYSSPDSNISSISSTTKEITKISRTPKQLLYECATAISEGNEEEASSMINNLRRIVSIQGEPSQRIAAYMVEGLAARLAESGKSIYRALKCKEPPSSDRLAAMQILFEVCPCFKFGFIAANYAITEAVKDEMKIHIIDFDINQGSQYITLIQTLASRSCKPSHVRLTGIDDPESVQRSVGGLKIIGQRLEQLAETLGLSFEFRAVSSRTSIVTSSMLDCRIGEALVVNFAFQLHHMPDESVSTVNERDQLLRLVKSLNPKLVTVVEQDVNTNTAPFLQRFVEAYNYYSAVFESLDVTLPRESQDRMNVERQCLARDIVNTVACEGEDRIERYEVAGKWRARMTMAGFSSSPMSTNVSDEIRKQVKLYCDRYKIKEEKGALHFGWEDKNLIVASAWR