AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34022

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34022.t1 MTR_1g095510 98.438 192 3 0 1 192 1 192 5.30e-143 395
MS.gene34022.t1 MTR_1g095510 88.021 192 3 1 1 192 1 172 2.45e-122 343
MS.gene34022.t1 MTR_1g095510 97.826 138 3 0 55 192 1 138 6.61e-102 290
MS.gene34022.t1 MTR_4g076200 65.644 163 55 1 27 189 18 179 3.33e-74 221
MS.gene34022.t1 MTR_5g009020 63.095 168 61 1 15 182 6 172 3.00e-73 219
MS.gene34022.t1 MTR_4g076200 67.626 139 44 1 51 189 1 138 3.63e-65 197
MS.gene34022.t1 MTR_1g021150 44.030 134 73 2 43 175 3 135 3.58e-33 116
MS.gene34022.t1 MTR_4g073240 46.400 125 66 1 43 167 3 126 1.15e-32 114
MS.gene34022.t1 MTR_4g108080 41.958 143 81 2 39 180 2 143 1.84e-32 114
MS.gene34022.t1 MTR_3g062450 44.882 127 69 1 43 169 3 128 7.09e-32 112
MS.gene34022.t1 MTR_5g075580 44.882 127 69 1 43 169 3 128 7.33e-32 112
MS.gene34022.t1 MTR_8g468530 41.259 143 82 2 39 180 18 159 9.06e-32 113
MS.gene34022.t1 MTR_2g078010 43.609 133 74 1 40 172 4 135 1.46e-31 112
MS.gene34022.t1 MTR_2g078010 42.857 133 75 1 40 172 4 135 1.70e-31 112
MS.gene34022.t1 MTR_7g116940 44.094 127 70 1 43 169 3 128 1.92e-31 111
MS.gene34022.t1 MTR_8g071040 41.791 134 76 2 43 175 3 135 2.28e-30 108
MS.gene34022.t1 MTR_1g021150 45.946 111 59 1 66 175 33 143 4.02e-30 108
MS.gene34022.t1 MTR_4g073240 48.454 97 50 0 71 167 1 97 1.58e-28 103
MS.gene34022.t1 MTR_5g075580 47.959 98 51 0 71 168 1 98 1.67e-28 103
MS.gene34022.t1 MTR_7g116940 41.791 134 70 2 43 169 3 135 2.56e-28 103
MS.gene34022.t1 MTR_3g098780 36.774 155 90 3 31 179 11 163 3.67e-28 105
MS.gene34022.t1 MTR_3g098780 38.235 136 81 2 45 179 6 139 5.54e-28 104
MS.gene34022.t1 MTR_2g078010 38.776 147 75 2 40 172 4 149 1.19e-27 102
MS.gene34022.t1 MTR_8g085690 44.262 122 64 2 37 155 15 135 4.39e-27 101
MS.gene34022.t1 MTR_4g108080 48.980 98 49 1 84 180 46 143 8.05e-26 97.4
MS.gene34022.t1 MTR_7g116940 42.727 110 62 1 43 152 3 111 2.84e-25 95.5
MS.gene34022.t1 MTR_4g108080 48.864 88 45 0 61 148 23 110 1.51e-24 93.2
MS.gene34022.t1 MTR_3g110110 40.952 105 61 1 58 161 21 125 9.03e-24 92.4
MS.gene34022.t1 MTR_2g078010 43.396 106 59 1 40 145 4 108 1.83e-23 90.1
MS.gene34022.t1 MTR_2g096690 39.062 128 64 2 67 180 30 157 4.95e-23 90.5
MS.gene34022.t1 MTR_4g063490 37.500 128 66 2 67 180 30 157 5.81e-23 90.5
MS.gene34022.t1 MTR_2g096690 38.583 127 65 1 67 180 30 156 9.07e-23 90.1
MS.gene34022.t1 MTR_5g016630 39.167 120 69 3 66 181 32 151 1.76e-20 84.0
MS.gene34022.t1 MTR_4g094872 39.130 115 67 2 70 181 37 151 4.39e-20 82.8
MS.gene34022.t1 MTR_4g106690 35.616 146 86 6 40 182 2 142 1.37e-18 79.7
MS.gene34022.t1 MTR_7g087590 30.921 152 101 3 41 191 10 158 2.18e-18 80.9
MS.gene34022.t1 MTR_8g028920 36.154 130 77 4 40 167 2 127 2.99e-18 78.6
MS.gene34022.t1 MTR_5g019480 33.962 106 70 0 65 170 54 159 3.49e-17 75.9
MS.gene34022.t1 MTR_5g019480 33.962 106 70 0 65 170 51 156 3.61e-17 75.9
MS.gene34022.t1 MTR_5g019480 33.962 106 70 0 65 170 3 108 4.08e-17 74.3
MS.gene34022.t1 MTR_8g076490 40.404 99 50 3 43 137 17 110 4.42e-15 69.7
MS.gene34022.t1 MTR_8g098595 35.870 92 57 2 78 167 21 112 1.02e-14 68.9
MS.gene34022.t1 MTR_8g028920 35.644 101 61 4 85 182 4 103 5.49e-14 66.6
MS.gene34022.t1 MTR_4g073240 55.556 54 24 0 107 160 4 57 6.43e-14 64.7
MS.gene34022.t1 MTR_5g021260 39.175 97 50 3 45 137 19 110 9.62e-14 66.2
MS.gene34022.t1 MTR_2g096690 46.154 65 35 0 67 131 30 94 6.80e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34022.t1 AT3G20060 66.438 146 48 1 37 182 32 176 6.96e-69 208
MS.gene34022.t1 AT1G50490 61.404 171 61 2 12 182 10 175 1.44e-63 194
MS.gene34022.t1 AT3G20060 67.424 132 42 1 51 182 1 131 3.68e-63 192
MS.gene34022.t1 AT2G16740 44.853 136 73 2 43 177 3 137 2.61e-34 119
MS.gene34022.t1 AT1G14400 42.254 142 80 2 39 179 2 142 1.07e-32 115
MS.gene34022.t1 AT1G14400 42.254 142 80 2 39 179 2 142 1.07e-32 115
MS.gene34022.t1 AT2G02760 41.958 143 81 2 39 180 2 143 1.46e-32 114
MS.gene34022.t1 AT2G02760 41.958 143 81 2 39 180 2 143 1.46e-32 114
MS.gene34022.t1 AT2G02760 41.958 143 81 2 39 180 2 143 1.46e-32 114
MS.gene34022.t1 AT2G02760 41.958 143 81 2 39 180 2 143 1.46e-32 114
MS.gene34022.t1 AT4G27960 41.497 147 81 2 43 185 3 148 4.37e-32 113
MS.gene34022.t1 AT1G16890 41.844 141 74 1 40 172 4 144 4.58e-32 114
MS.gene34022.t1 AT5G41700 43.307 127 72 0 43 169 3 129 5.96e-32 113
MS.gene34022.t1 AT1G16890 43.609 133 74 1 40 172 4 135 6.05e-32 113
MS.gene34022.t1 AT1G64230 44.882 127 69 1 43 169 3 128 7.27e-32 112
MS.gene34022.t1 AT1G64230 44.882 127 69 1 43 169 3 128 7.27e-32 112
MS.gene34022.t1 AT5G53300 41.497 147 81 2 43 185 3 148 9.63e-32 112
MS.gene34022.t1 AT5G53300 41.497 147 81 2 43 185 3 148 9.63e-32 112
MS.gene34022.t1 AT5G53300 41.497 147 81 2 43 185 3 148 9.63e-32 112
MS.gene34022.t1 AT5G62540 43.262 141 78 2 39 178 2 141 1.11e-31 112
MS.gene34022.t1 AT5G56150 42.857 126 71 1 43 168 3 127 1.22e-31 112
MS.gene34022.t1 AT1G78870 43.609 133 74 1 40 172 4 135 2.10e-31 112
MS.gene34022.t1 AT5G41700 44.094 127 70 1 43 169 3 128 2.41e-31 111
MS.gene34022.t1 AT5G41700 44.094 127 70 1 43 169 3 128 2.41e-31 111
MS.gene34022.t1 AT5G41700 44.094 127 70 1 43 169 3 128 2.41e-31 111
MS.gene34022.t1 AT3G08690 44.444 126 69 1 43 168 3 127 3.00e-31 111
MS.gene34022.t1 AT3G08690 44.444 126 69 1 43 168 3 127 3.00e-31 111
MS.gene34022.t1 AT4G27960 41.497 147 81 2 43 185 33 178 9.24e-31 110
MS.gene34022.t1 AT1G64230 42.336 137 67 2 43 169 3 137 1.57e-30 109
MS.gene34022.t1 AT1G64230 47.115 104 55 0 66 169 67 170 1.74e-29 108
MS.gene34022.t1 AT1G64230 47.115 104 55 0 66 169 67 170 1.74e-29 108
MS.gene34022.t1 AT5G50870 41.600 125 72 1 45 168 6 130 6.73e-29 106
MS.gene34022.t1 AT3G08700 42.143 140 78 3 43 180 3 141 7.84e-29 105
MS.gene34022.t1 AT3G08700 42.143 140 78 3 43 180 3 141 7.84e-29 105
MS.gene34022.t1 AT5G50870 42.276 123 70 1 45 166 6 128 8.95e-29 106
MS.gene34022.t1 AT1G16890 50.526 95 47 0 78 172 8 102 2.00e-28 103
MS.gene34022.t1 AT1G78870 50.526 95 47 0 78 172 8 102 2.02e-28 103
MS.gene34022.t1 AT1G78870 50.000 96 47 1 78 172 8 103 5.61e-27 99.8
MS.gene34022.t1 AT3G13550 40.876 137 76 3 40 172 32 167 1.64e-26 100
MS.gene34022.t1 AT1G36340 42.017 119 67 1 42 160 9 125 2.97e-26 98.6
MS.gene34022.t1 AT1G36340 42.017 119 67 1 42 160 9 125 2.97e-26 98.6
MS.gene34022.t1 AT3G13550 42.373 118 64 2 40 154 32 148 6.43e-25 95.9
MS.gene34022.t1 AT5G41700 43.396 106 59 1 43 148 3 107 1.08e-24 94.4
MS.gene34022.t1 AT5G25760 42.857 105 59 1 58 161 21 125 1.78e-24 94.4
MS.gene34022.t1 AT5G25760 42.857 105 59 1 58 161 21 125 1.78e-24 94.4
MS.gene34022.t1 AT5G56150 44.211 95 52 1 43 137 3 96 2.14e-24 92.4
MS.gene34022.t1 AT5G53300 46.316 95 50 1 43 137 3 96 3.62e-24 92.0
MS.gene34022.t1 AT1G78870 42.727 110 62 1 40 149 4 112 1.19e-23 90.9
MS.gene34022.t1 AT5G59300 39.062 128 64 2 67 180 30 157 4.23e-23 90.9
MS.gene34022.t1 AT3G46460 38.281 128 65 2 67 180 30 157 8.66e-22 87.4
MS.gene34022.t1 AT3G55380 35.156 128 69 2 67 180 31 158 3.58e-21 85.9
MS.gene34022.t1 AT1G63800 38.462 130 74 4 40 167 2 127 3.32e-19 81.3
MS.gene34022.t1 AT3G57870 38.017 121 69 3 70 184 38 158 2.79e-18 78.2
MS.gene34022.t1 AT5G41340 36.923 130 76 4 40 167 2 127 3.23e-18 78.6
MS.gene34022.t1 AT2G32790 39.344 122 73 1 63 183 51 172 3.82e-18 78.2
MS.gene34022.t1 AT2G18600 32.540 126 82 3 64 187 52 176 7.98e-18 77.4
MS.gene34022.t1 AT2G18600 32.540 126 82 3 64 187 52 176 7.98e-18 77.4
MS.gene34022.t1 AT5G05080 33.835 133 87 1 41 173 10 141 1.20e-17 78.6
MS.gene34022.t1 AT5G05080 33.835 133 87 1 41 173 10 141 1.20e-17 78.6
MS.gene34022.t1 AT5G05080 33.835 133 87 1 41 173 10 141 1.20e-17 78.6
MS.gene34022.t1 AT5G05080 33.835 133 87 1 41 173 10 141 1.20e-17 78.6
MS.gene34022.t1 AT3G24515 37.594 133 67 5 37 155 2 132 8.68e-17 77.8
MS.gene34022.t1 AT4G36800 33.333 108 70 2 64 170 51 157 9.49e-17 74.7
MS.gene34022.t1 AT4G36800 33.333 108 70 2 64 170 51 157 9.49e-17 74.7
MS.gene34022.t1 AT2G46030 35.385 130 78 4 40 167 2 127 3.94e-16 73.2
MS.gene34022.t1 AT2G46030 35.385 130 78 4 40 167 2 127 3.94e-16 73.2
MS.gene34022.t1 AT3G46460 36.111 108 55 2 87 180 8 115 5.96e-16 71.2
MS.gene34022.t1 AT3G46460 36.111 108 55 2 87 180 8 115 5.96e-16 71.2
MS.gene34022.t1 AT2G46030 34.507 142 79 5 28 167 70 199 1.41e-15 72.8
MS.gene34022.t1 AT1G63800 37.931 116 66 4 54 167 1 112 2.39e-15 70.9
MS.gene34022.t1 AT3G55380 29.012 162 67 4 67 180 31 192 2.65e-15 71.2
MS.gene34022.t1 AT3G24515 38.889 90 51 1 70 155 68 157 8.06e-15 72.0
MS.gene34022.t1 AT4G36410 38.835 103 54 3 39 137 13 110 1.00e-14 68.9
MS.gene34022.t1 AT1G75440 39.394 99 51 3 43 137 17 110 7.77e-14 66.6
MS.gene34022.t1 AT5G41340 39.286 84 49 2 86 167 9 92 1.15e-13 65.9
MS.gene34022.t1 AT5G42990 38.144 97 51 3 45 137 19 110 1.22e-12 63.2
MS.gene34022.t1 AT2G46030 35.345 116 69 4 54 167 19 130 1.40e-12 63.5
MS.gene34022.t1 AT2G46030 35.345 116 69 4 54 167 19 130 1.40e-12 63.5
MS.gene34022.t1 AT1G45050 38.144 97 51 3 45 137 19 110 1.75e-12 62.8

Find 56 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTTGGGGTGGTTTAAA+AGG 0.191154 1.4:+74208465 None:intergenic
AATTGTATAAATCAACTTTC+AGG 0.222701 1.4:+74207600 None:intergenic
CATCCAAATGTTGACCAGTT+TGG 0.280045 1.4:-74208423 MS.gene34022:CDS
CAGACTACAGAAGGAATTAA+TGG 0.312893 1.4:-74208984 MS.gene34022:CDS
TTTAGAGGGCTCTCCAAGTT+AGG 0.344635 1.4:+74207968 None:intergenic
AGGAGAAGCGAGAGGCTTCT+TGG 0.357261 1.4:+74209301 None:intergenic
CGACTGGCCGCCGATTCAAC+AGG 0.365523 1.4:+74209341 None:intergenic
TCTGCAGCATAGTTGTTTAG+AGG 0.381020 1.4:+74207953 None:intergenic
TTGCCAAACTGGTCAACATT+TGG 0.388830 1.4:+74208420 None:intergenic
CTAGACAAATGTTGCCAAAC+TGG 0.389058 1.4:+74208409 None:intergenic
AGAAAATTCCCCTGTTGAAT+CGG 0.394486 1.4:-74209351 MS.gene34022:CDS
GGTGGTTTAAAAGGATAGTT+CGG 0.395297 1.4:+74208474 None:intergenic
ACAACTATGCTGCAGAGCTT+TGG 0.406851 1.4:-74207946 MS.gene34022:CDS
ACATGGATTGGCACAATTCA+AGG 0.410583 1.4:-74208552 MS.gene34022:CDS
GTGGTTTAAAAGGATAGTTC+GGG 0.414728 1.4:+74208475 None:intergenic
TCTGTCTATACAAAGTCTAT+TGG 0.417973 1.4:-74208072 MS.gene34022:CDS
CTTCGGCGGTGGTGTTTGAT+CGG 0.437236 1.4:+74209372 None:intergenic
CAGATGAGTGGAGGAGATCT+TGG 0.446524 1.4:-74208609 MS.gene34022:CDS
CATTTGTCTAGACATCCTTC+AGG 0.450655 1.4:-74208397 MS.gene34022:intron
GGCACAATTCAAGGTGGAAA+AGG 0.458217 1.4:-74208543 MS.gene34022:CDS
TCTAACAGCAGAGCCTAACT+TGG 0.460379 1.4:-74207981 MS.gene34022:intron
TGGAAAAGGAACTCCTTATG+AGG 0.468163 1.4:-74208529 MS.gene34022:CDS
GGATGATCGACGGTGGCGAC+TGG 0.470050 1.4:+74209325 None:intergenic
GGAGTATCTGCTTTCCCTGA+TGG 0.470606 1.4:-74208588 MS.gene34022:CDS
GTCCACAAGCAGTATTTAGC+TGG 0.486859 1.4:-74207636 MS.gene34022:CDS
ATAAATCAACTTTCAGGTGC+AGG 0.493849 1.4:+74207606 None:intergenic
TTATAAGATAAACCCTCATA+AGG 0.500118 1.4:+74208516 None:intergenic
TCATGCAGATTTCAAGAGAA+TGG 0.510290 1.4:-74207658 MS.gene34022:intron
GTCTCAAATTTCACTTGGGG+TGG 0.517777 1.4:+74208456 None:intergenic
TGCAGAGCTTTGGAATGATA+AGG 0.522423 1.4:-74207936 MS.gene34022:CDS
GAAGGAATTAATGGCTCTCA+TGG 0.522770 1.4:-74208975 MS.gene34022:intron
GAAACGGAAGTTGTGTCAGC+AGG 0.527352 1.4:+74209275 None:intergenic
GAAGTTGTGTCAGCAGGAAG+AGG 0.534120 1.4:+74209281 None:intergenic
CGTCGTCTCAAATTTCACTT+GGG 0.535928 1.4:+74208452 None:intergenic
GGAGATGAACCTTTGAGAAA+CGG 0.539253 1.4:+74209259 None:intergenic
ACGTCGTCTCAAATTTCACT+TGG 0.545034 1.4:+74208451 None:intergenic
GTTCAATTGCAGACTACAGA+AGG 0.551110 1.4:-74208993 MS.gene34022:intron
CTGCAGCATAGTTGTTTAGA+GGG 0.556199 1.4:+74207954 None:intergenic
ACACAACTTCCGTTTCTCAA+AGG 0.558610 1.4:-74209268 MS.gene34022:intron
TGGTTTAAAAGGATAGTTCG+GGG 0.571786 1.4:+74208476 None:intergenic
CTCCAGCTAAATACTGCTTG+TGG 0.577463 1.4:+74207634 None:intergenic
AGAAGCGAGAGGCTTCTTGG+AGG 0.590943 1.4:+74209304 None:intergenic
TTGTGATTGTATAGGACAAG+TGG 0.591402 1.4:-74208124 MS.gene34022:intron
GGAAAAGGAACTCCTTATGA+GGG 0.594287 1.4:-74208528 MS.gene34022:CDS
GGTTTAAAAGGATAGTTCGG+GGG 0.602685 1.4:+74208477 None:intergenic
TCTATACAAAGTCTATTGGA+AGG 0.607196 1.4:-74208068 MS.gene34022:intron
AAATTCCCCTGTTGAATCGG+CGG 0.617461 1.4:-74209348 MS.gene34022:CDS
GACTGGCCGCCGATTCAACA+GGG 0.618918 1.4:+74209342 None:intergenic
GAGCTTTGGAATGATAAGGA+AGG 0.647349 1.4:-74207932 MS.gene34022:intron
GCAGGAAGAGGAGAAGCGAG+AGG 0.663633 1.4:+74209293 None:intergenic
GCTTCTTGGAGGATGATCGA+CGG 0.669189 1.4:+74209315 None:intergenic
GTTTAAAAGGATAGTTCGGG+GGG 0.670883 1.4:+74208478 None:intergenic
GTCGTCTCAAATTTCACTTG+GGG 0.674521 1.4:+74208453 None:intergenic
ACTGGCCGCCGATTCAACAG+GGG 0.680091 1.4:+74209343 None:intergenic
TCTTGGAGGATGATCGACGG+TGG 0.707980 1.4:+74209318 None:intergenic
TGGATTGGCACAATTCAAGG+TGG 0.712983 1.4:-74208549 MS.gene34022:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAATTTAGAAATATTTA+AGG + chr1.4:74207763-74207782 None:intergenic 10.0%
!! TGAAATTTAGAAATATTTAA+GGG + chr1.4:74207762-74207781 None:intergenic 10.0%
!! AAATTTAGAAATATTTAAGG+GGG + chr1.4:74207760-74207779 None:intergenic 15.0%
!! GAAATTTAGAAATATTTAAG+GGG + chr1.4:74207761-74207780 None:intergenic 15.0%
!! GCATTCATATTAAATATAAT+TGG + chr1.4:74208804-74208823 None:intergenic 15.0%
!! TCATTAAGAAACTAAATTTA+TGG - chr1.4:74208280-74208299 MS.gene34022:intron 15.0%
!!! CAGTCAATTTTTTTATTTTT+AGG - chr1.4:74208064-74208083 MS.gene34022:intron 15.0%
!!! TTAAATTTTAAACTTTGATG+TGG - chr1.4:74207931-74207950 MS.gene34022:intron 15.0%
!! AAAAAAACTACATTAGAAGA+TGG + chr1.4:74207789-74207808 None:intergenic 20.0%
!! AATTTAGAAATATTTAAGGG+GGG + chr1.4:74207759-74207778 None:intergenic 20.0%
!! AGATCAATTGAACTAAAATT+TGG + chr1.4:74209176-74209195 None:intergenic 20.0%
!! TAAAAAAATCACATAATCCA+GGG - chr1.4:74208716-74208735 MS.gene34022:intron 20.0%
!! TCATCTACATATTTAATACT+AGG + chr1.4:74208826-74208845 None:intergenic 20.0%
!! TTAAAAAAATCACATAATCC+AGG - chr1.4:74208715-74208734 MS.gene34022:intron 20.0%
! AATCATGTGAAATATGTTGT+TGG - chr1.4:74208243-74208262 MS.gene34022:intron 25.0%
! ACACCATAAATTTGACAATA+AGG + chr1.4:74208090-74208109 None:intergenic 25.0%
! AGTTTCTTAATGATACAAGA+AGG + chr1.4:74208273-74208292 None:intergenic 25.0%
! ATCAAATTTCATTTCATGCA+TGG + chr1.4:74207881-74207900 None:intergenic 25.0%
! ATCATGTGAAATATGTTGTT+GGG - chr1.4:74208244-74208263 MS.gene34022:intron 25.0%
! ATTGTCAAATTTATGGTGTT+TGG - chr1.4:74208091-74208110 MS.gene34022:CDS 25.0%
! GTACTTTACTTCTTCATATT+AGG - chr1.4:74208214-74208233 MS.gene34022:intron 25.0%
! TAAAAATATTCTCACCATCA+GGG + chr1.4:74208420-74208439 None:intergenic 25.0%
! TTACTTCTTCATATTAGGTT+GGG - chr1.4:74208219-74208238 MS.gene34022:intron 25.0%
! TTATAAGATAAACCCTCATA+AGG + chr1.4:74208478-74208497 None:intergenic 25.0%
! TTTACTTCTTCATATTAGGT+TGG - chr1.4:74208218-74208237 MS.gene34022:intron 25.0%
!!! ATGGTGAGAATATTTTTACA+TGG - chr1.4:74208422-74208441 MS.gene34022:CDS 25.0%
!!! GAGAATATTTTTACATGGAT+TGG - chr1.4:74208427-74208446 MS.gene34022:CDS 25.0%
!!! GGTTTTAATCTTTGTAGATT+TGG - chr1.4:74209271-74209290 MS.gene34022:CDS 25.0%
!!! TTTTTAGTCTTGATCAGTTT+TGG - chr1.4:74207805-74207824 MS.gene34022:intron 25.0%
CTAAATTCATGAATACCTGA+AGG + chr1.4:74208612-74208631 None:intergenic 30.0%
GAGCAATAACAACAAATAGT+TGG + chr1.4:74209146-74209165 None:intergenic 30.0%
GTAAAAATATTCTCACCATC+AGG + chr1.4:74208421-74208440 None:intergenic 30.0%
TAGAAAATAACTGATCAGAC+AGG + chr1.4:74208994-74209013 None:intergenic 30.0%
TCTATACAAAGTCTATTGGA+AGG - chr1.4:74208923-74208942 MS.gene34022:intron 30.0%
TCTGTCTATACAAAGTCTAT+TGG - chr1.4:74208919-74208938 MS.gene34022:intron 30.0%
TTAAACTATTATCCGTGTGT+CGG + chr1.4:74208699-74208718 None:intergenic 30.0%
TTCCTACTTTGTGATTGTAT+AGG - chr1.4:74208859-74208878 MS.gene34022:intron 30.0%
!! AGGCCTTATTGTCAAATTTA+TGG - chr1.4:74208084-74208103 MS.gene34022:CDS 30.0%
AGAAAATTCCCCTGTTGAAT+CGG - chr1.4:74207640-74207659 MS.gene34022:CDS 35.0%
CAGACTACAGAAGGAATTAA+TGG - chr1.4:74208007-74208026 MS.gene34022:intron 35.0%
GGTGGTTTAAAAGGATAGTT+CGG + chr1.4:74208520-74208539 None:intergenic 35.0%
GTCCTATACAATCACAAAGT+AGG + chr1.4:74208864-74208883 None:intergenic 35.0%
GTGGTTTAAAAGGATAGTTC+GGG + chr1.4:74208519-74208538 None:intergenic 35.0%
TCAATGACTCTATTCCTTGA+GGG - chr1.4:74209201-74209220 MS.gene34022:intron 35.0%
TCATGCAGATTTCAAGAGAA+TGG - chr1.4:74209333-74209352 MS.gene34022:CDS 35.0%
TGGTTTAAAAGGATAGTTCG+GGG + chr1.4:74208518-74208537 None:intergenic 35.0%
TTAGAAATATTTAAGGGGGG+AGG + chr1.4:74207756-74207775 None:intergenic 35.0%
TTCAATGACTCTATTCCTTG+AGG - chr1.4:74209200-74209219 MS.gene34022:intron 35.0%
TTCATTCGTTCTCACAAAGA+CGG - chr1.4:74208324-74208343 MS.gene34022:intron 35.0%
TTGTGATTGTATAGGACAAG+TGG - chr1.4:74208867-74208886 MS.gene34022:intron 35.0%
! AGTAAAGTACTAAGTATGCG+TGG + chr1.4:74208204-74208223 None:intergenic 35.0%
! TTCAACAGGGGAATTTTCTT+CGG + chr1.4:74207639-74207658 None:intergenic 35.0%
! TTTGGAATGATAAGGAAGGT+TGG - chr1.4:74209063-74209082 MS.gene34022:intron 35.0%
!! GGTAATGGAAAAAAGAAGCA+CGG + chr1.4:74208757-74208776 None:intergenic 35.0%
!!! TTACTTTTTGTGCAGATGAG+TGG - chr1.4:74208370-74208389 MS.gene34022:intron 35.0%
ACACAACTTCCGTTTCTCAA+AGG - chr1.4:74207723-74207742 MS.gene34022:intron 40.0%
ACATAATCCAGGGTAATCTG+AGG - chr1.4:74208726-74208745 MS.gene34022:intron 40.0%
ACGTCGTCTCAAATTTCACT+TGG + chr1.4:74208543-74208562 None:intergenic 40.0%
AGCAGTAAACACACAGGTAA+TGG + chr1.4:74208772-74208791 None:intergenic 40.0%
ATCCAAAGCAGTAAACACAC+AGG + chr1.4:74208778-74208797 None:intergenic 40.0%
CATCCAAATGTTGACCAGTT+TGG - chr1.4:74208568-74208587 MS.gene34022:CDS 40.0%
CATTTGTCTAGACATCCTTC+AGG - chr1.4:74208594-74208613 MS.gene34022:CDS 40.0%
CGTCGTCTCAAATTTCACTT+GGG + chr1.4:74208542-74208561 None:intergenic 40.0%
CTAGACAAATGTTGCCAAAC+TGG + chr1.4:74208585-74208604 None:intergenic 40.0%
CTAGTGCTCTAGTAGATTGT+AGG - chr1.4:74209250-74209269 MS.gene34022:intron 40.0%
CTGCAGCATAGTTGTTTAGA+GGG + chr1.4:74209040-74209059 None:intergenic 40.0%
GAAGGAATTAATGGCTCTCA+TGG - chr1.4:74208016-74208035 MS.gene34022:intron 40.0%
GGAAAAGGAACTCCTTATGA+GGG - chr1.4:74208463-74208482 MS.gene34022:CDS 40.0%
GGTTTAAAAGGATAGTTCGG+GGG + chr1.4:74208517-74208536 None:intergenic 40.0%
GTCGTCTCAAATTTCACTTG+GGG + chr1.4:74208541-74208560 None:intergenic 40.0%
GTTCAATTGCAGACTACAGA+AGG - chr1.4:74207998-74208017 MS.gene34022:intron 40.0%
GTTTAAAAGGATAGTTCGGG+GGG + chr1.4:74208516-74208535 None:intergenic 40.0%
TACCTGTGTGTTTACTGCTT+TGG - chr1.4:74208773-74208792 MS.gene34022:intron 40.0%
TAGTTACCCACTATGAAGCA+CGG + chr1.4:74208176-74208195 None:intergenic 40.0%
TCTGCAGCATAGTTGTTTAG+AGG + chr1.4:74209041-74209060 None:intergenic 40.0%
TGGAAAAGGAACTCCTTATG+AGG - chr1.4:74208462-74208481 MS.gene34022:CDS 40.0%
TTCACTTGGGGTGGTTTAAA+AGG + chr1.4:74208529-74208548 None:intergenic 40.0%
TTGCCAAACTGGTCAACATT+TGG + chr1.4:74208574-74208593 None:intergenic 40.0%
! GGAGATGAACCTTTGAGAAA+CGG + chr1.4:74207735-74207754 None:intergenic 40.0%
! GTTTGGTAACCTATGAAGCA+CGG - chr1.4:74208108-74208127 MS.gene34022:CDS 40.0%
! TCATCAGCATTTAGAGTGAG+TGG + chr1.4:74208350-74208369 None:intergenic 40.0%
!! ACATGGATTGGCACAATTCA+AGG - chr1.4:74208439-74208458 MS.gene34022:CDS 40.0%
!! GAGCTTTGGAATGATAAGGA+AGG - chr1.4:74209059-74209078 MS.gene34022:intron 40.0%
!! TGCAGAGCTTTGGAATGATA+AGG - chr1.4:74209055-74209074 MS.gene34022:intron 40.0%
!!! ATTTTAACTGGTGCTGCTCA+TGG - chr1.4:74209304-74209323 MS.gene34022:CDS 40.0%
!!! GGTCGAATGCGTATTTTAAC+TGG - chr1.4:74209292-74209311 MS.gene34022:CDS 40.0%
!!! TGCAAGTTTTAGAACCCTCA+AGG + chr1.4:74209218-74209237 None:intergenic 40.0%
AAATTCCCCTGTTGAATCGG+CGG - chr1.4:74207643-74207662 MS.gene34022:CDS 45.0%
AAGTGTCCGTGCTTCATAGT+GGG - chr1.4:74208167-74208186 MS.gene34022:intron 45.0%
ACAACTATGCTGCAGAGCTT+TGG - chr1.4:74209045-74209064 MS.gene34022:intron 45.0%
ACTGACACAAACACAGACAC+CGG - chr1.4:74208665-74208684 MS.gene34022:intron 45.0%
ATGAAGCACGGATACAGACA+CGG - chr1.4:74208120-74208139 MS.gene34022:intron 45.0%
CTCCAGCTAAATACTGCTTG+TGG + chr1.4:74209360-74209379 None:intergenic 45.0%
CTGATCAGACAGGAACACAA+AGG + chr1.4:74208984-74209003 None:intergenic 45.0%
GGCACAATTCAAGGTGGAAA+AGG - chr1.4:74208448-74208467 MS.gene34022:CDS 45.0%
GTCCACAAGCAGTATTTAGC+TGG - chr1.4:74209355-74209374 MS.gene34022:CDS 45.0%
GTCTCAAATTTCACTTGGGG+TGG + chr1.4:74208538-74208557 None:intergenic 45.0%
TATGAAGCACGGACACTTCT+TGG + chr1.4:74208165-74208184 None:intergenic 45.0%
TCTAACAGCAGAGCCTAACT+TGG - chr1.4:74209010-74209029 MS.gene34022:intron 45.0%
TGTCTGTATCCGTGCTTCAT+AGG + chr1.4:74208120-74208139 None:intergenic 45.0%
TTTAGAGGGCTCTCCAAGTT+AGG + chr1.4:74209026-74209045 None:intergenic 45.0%
! AACAGGGGAATTTTCTTCGG+CGG + chr1.4:74207636-74207655 None:intergenic 45.0%
! CTTTTTGTGCAGATGAGTGG+AGG - chr1.4:74208373-74208392 MS.gene34022:intron 45.0%
!! TGGATTGGCACAATTCAAGG+TGG - chr1.4:74208442-74208461 MS.gene34022:CDS 45.0%
ATAGTTCGGGGGGAAACGTA+AGG + chr1.4:74208506-74208525 None:intergenic 50.0%
CAGATGAGTGGAGGAGATCT+TGG - chr1.4:74208382-74208401 MS.gene34022:intron 50.0%
GAAACGGAAGTTGTGTCAGC+AGG + chr1.4:74207719-74207738 None:intergenic 50.0%
GAAGTTGTGTCAGCAGGAAG+AGG + chr1.4:74207713-74207732 None:intergenic 50.0%
GCACGGACACTTCTTGGTTT+AGG + chr1.4:74208159-74208178 None:intergenic 50.0%
GCTTCTTGGAGGATGATCGA+CGG + chr1.4:74207679-74207698 None:intergenic 50.0%
TTCTTGGTTTAGGCGTGTCC+TGG + chr1.4:74208149-74208168 None:intergenic 50.0%
! GAAGTGTCCGTGCTTCATAG+TGG - chr1.4:74208166-74208185 MS.gene34022:intron 50.0%
! GGAGTATCTGCTTTCCCTGA+TGG - chr1.4:74208403-74208422 MS.gene34022:CDS 50.0%
AGAAGCGAGAGGCTTCTTGG+AGG + chr1.4:74207690-74207709 None:intergenic 55.0%
AGGAGAAGCGAGAGGCTTCT+TGG + chr1.4:74207693-74207712 None:intergenic 55.0%
CTTCGGCGGTGGTGTTTGAT+CGG + chr1.4:74207622-74207641 None:intergenic 55.0%
GGACACTCCTCAGATTACCC+TGG + chr1.4:74208736-74208755 None:intergenic 55.0%
TCTTGGAGGATGATCGACGG+TGG + chr1.4:74207676-74207695 None:intergenic 55.0%
! AGGGGAATTTTCTTCGGCGG+TGG + chr1.4:74207633-74207652 None:intergenic 55.0%
CCGGACACAACACCGACACA+CGG - chr1.4:74208684-74208703 MS.gene34022:intron 60.0%
CGGATACAGACACGGACACC+AGG - chr1.4:74208128-74208147 MS.gene34022:intron 60.0%
GCAGGAAGAGGAGAAGCGAG+AGG + chr1.4:74207701-74207720 None:intergenic 60.0%
!! ACTGGCCGCCGATTCAACAG+GGG + chr1.4:74207651-74207670 None:intergenic 60.0%
!! CCGTGTGTCGGTGTTGTGTC+CGG + chr1.4:74208687-74208706 None:intergenic 60.0%
!! GACTGGCCGCCGATTCAACA+GGG + chr1.4:74207652-74207671 None:intergenic 60.0%
GGATGATCGACGGTGGCGAC+TGG + chr1.4:74207669-74207688 None:intergenic 65.0%
!! CGACTGGCCGCCGATTCAAC+AGG + chr1.4:74207653-74207672 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74207611 74209402 74207611 ID=MS.gene34022
chr1.4 mRNA 74207611 74209402 74207611 ID=MS.gene34022.t1;Parent=MS.gene34022
chr1.4 exon 74209269 74209402 74209269 ID=MS.gene34022.t1.exon1;Parent=MS.gene34022.t1
chr1.4 CDS 74209269 74209402 74209269 ID=cds.MS.gene34022.t1;Parent=MS.gene34022.t1
chr1.4 exon 74208976 74209003 74208976 ID=MS.gene34022.t1.exon2;Parent=MS.gene34022.t1
chr1.4 CDS 74208976 74209003 74208976 ID=cds.MS.gene34022.t1;Parent=MS.gene34022.t1
chr1.4 exon 74208398 74208628 74208398 ID=MS.gene34022.t1.exon3;Parent=MS.gene34022.t1
chr1.4 CDS 74208398 74208628 74208398 ID=cds.MS.gene34022.t1;Parent=MS.gene34022.t1
chr1.4 exon 74208069 74208132 74208069 ID=MS.gene34022.t1.exon4;Parent=MS.gene34022.t1
chr1.4 CDS 74208069 74208132 74208069 ID=cds.MS.gene34022.t1;Parent=MS.gene34022.t1
chr1.4 exon 74207933 74207992 74207933 ID=MS.gene34022.t1.exon5;Parent=MS.gene34022.t1
chr1.4 CDS 74207933 74207992 74207933 ID=cds.MS.gene34022.t1;Parent=MS.gene34022.t1
chr1.4 exon 74207611 74207672 74207611 ID=MS.gene34022.t1.exon6;Parent=MS.gene34022.t1
chr1.4 CDS 74207611 74207672 74207611 ID=cds.MS.gene34022.t1;Parent=MS.gene34022.t1
Gene Sequence

>MS.gene34022

ATGGAGGTCCGATCAAACACCACCGCCGAAGAAAATTCCCCTGTTGAATCGGCGGCCAGTCGCCACCGTCGATCATCCTCCAAGAAGCCTCTCGCTTCTCCTCTTCCTGCTGACACAACTTCCGTTTCTCAAAGGTTCATCTCCTCCCCCCTTAAATATTTCTAAATTTCATTTTCCATCTTCTAATGTAGTTTTTTTTAGTCTTGATCAGTTTTGGATTTGTTATTCATTGCAAACACATTTGAAGCTACAAATTTTGAGTAGAATCCATGCATGAAATGAAATTTGATTATCGTGTGTTCATTTTTACTCTTTTTATTTTAAATTTTAAACTTTGATGTGGTAGAATAGATTTTCATAGTTTCATTAATTCTATTTAATTTTTTTGTTCAATTGCAGACTACAGAAGGAATTAATGGCTCTCATGGTATGTATGTCTCTCTTTGCTGTATACAGTCAATTTTTTTATTTTTAGGCCTTATTGTCAAATTTATGGTGTTTGGTAACCTATGAAGCACGGATACAGACACGGACACCAGGACACGCCTAAACCAAGAAGTGTCCGTGCTTCATAGTGGGTAACTATTCAACCACGCATACTTAGTACTTTACTTCTTCATATTAGGTTGGGAAATCATGTGAAATATGTTGTTGGGTAACCTTCTTGTATCATTAAGAAACTAAATTTATGGTTGCTTTCTTTCTATTCAATATTCATTCGTTCTCACAAAGACGGCCACTCACTCTAAATGCTGATGATTACTTTTTGTGCAGATGAGTGGAGGAGATCTTGGAGTATCTGCTTTCCCTGATGGTGAGAATATTTTTACATGGATTGGCACAATTCAAGGTGGAAAAGGAACTCCTTATGAGGGTTTATCTTATAAACTTTCCTTACGTTTCCCCCCGAACTATCCTTTTAAACCACCCCAAGTGAAATTTGAGACGACGTGCTTTCATCCAAATGTTGACCAGTTTGGCAACATTTGTCTAGACATCCTTCAGGTATTCATGAATTTAGAGAACAAAATAGCAAATACTAGAGCTATGAAGCACTGACACAAACACAGACACCGGACACAACACCGACACACGGATAATAGTTTAAAAAAATCACATAATCCAGGGTAATCTGAGGAGTGTCCGTGCTTCTTTTTTCCATTACCTGTGTGTTTACTGCTTTGGATCTACCAATTATATTTAATATGAATGCCTAGTATTAAATATGTAGATGAAATATTCATTTCTTTCCTACTTTGTGATTGTATAGGACAAGTGGTCTTCAGCTTATGACTGTAGAACTATGCTTCTGTCTATACAAAGTCTATTGGAAGGTGTGTTCTTCATTTATTTTATGAGTTTTGCAGTCACCTTTGTGTTCCTGTCTGATCAGTTATTTTCTAACAGCAGAGCCTAACTTGGAGAGCCCTCTAAACAACTATGCTGCAGAGCTTTGGAATGATAAGGAAGGTTGGAAATTTTTTTCATTCTAACTAAAGCACATTTGTTTTCTGATTTAATTATTTAAACTTCCAACTATTTGTTGTTATTGCTCAGTTGCTCCAAATTTTAGTTCAATTGATCTCTATTTCAATGACTCTATTCCTTGAGGGTTCTAAAACTTGCATTACTTTAATATCTAGTGCTCTAGTAGATTGTAGGTTTTAATCTTTGTAGATTTGGTCGAATGCGTATTTTAACTGGTGCTGCTCATGGAATATATCATGCAGATTTCAAGAGAATGGTCCACAAGCAGTATTTAGCTGGAGAAGCACCTGCACCTGAAAGTTGA
Protein sequence

>MS.gene34022.t1

MEVRSNTTAEENSPVESAASRHRRSSSKKPLASPLPADTTSVSQRLQKELMALMMSGGDLGVSAFPDGENIFTWIGTIQGGKGTPYEGLSYKLSLRFPPNYPFKPPQVKFETTCFHPNVDQFGNICLDILQDKWSSAYDCRTMLLSIQSLLEEPNLESPLNNYAAELWNDKEDFKRMVHKQYLAGEAPAPES