AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34954

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34954.t1 MTR_1g033420 77.116 319 71 2 6 323 7 324 0.0 533
MS.gene34954.t1 MTR_5g029100 56.610 295 120 3 20 308 31 323 5.76e-124 360
MS.gene34954.t1 MTR_5g029100 59.514 247 92 3 62 302 42 286 9.53e-112 327
MS.gene34954.t1 MTR_8g090100 48.084 287 142 5 20 302 10 293 4.40e-96 288
MS.gene34954.t1 MTR_8g064180 45.425 306 139 5 7 290 11 310 8.50e-90 271
MS.gene34954.t1 MTR_4g057450 41.528 301 153 6 5 290 12 304 2.81e-72 226
MS.gene34954.t1 MTR_1g063020 40.134 299 154 7 8 290 5 294 1.33e-66 211
MS.gene34954.t1 MTR_2g095800 40.333 300 156 8 6 290 18 309 2.46e-65 208
MS.gene34954.t1 MTR_8g013055 40.602 266 143 7 31 291 31 286 7.57e-64 204
MS.gene34954.t1 MTR_7g102260 38.384 297 160 6 9 290 3 291 4.42e-62 199
MS.gene34954.t1 MTR_7g084750 36.972 284 171 5 9 290 10 287 1.41e-60 196
MS.gene34954.t1 MTR_1g105495 36.486 296 182 5 4 296 8 300 2.52e-60 195
MS.gene34954.t1 MTR_6g033085 36.824 296 174 8 1 291 1 288 1.44e-58 191
MS.gene34954.t1 MTR_4g045853 37.667 300 162 11 4 291 3 289 3.77e-58 189
MS.gene34954.t1 MTR_3g089540 40.613 261 146 5 31 290 30 282 7.27e-58 189
MS.gene34954.t1 MTR_4g128410 37.884 293 161 9 6 290 1 280 8.60e-58 188
MS.gene34954.t1 MTR_1g105900 37.358 265 158 4 31 292 34 293 8.94e-58 189
MS.gene34954.t1 MTR_2g089140 37.333 300 162 11 4 291 2 287 1.54e-57 188
MS.gene34954.t1 MTR_7g084760 37.282 287 166 6 9 290 10 287 5.04e-57 187
MS.gene34954.t1 MTR_5g091730 38.168 262 149 5 31 290 28 278 7.35e-57 186
MS.gene34954.t1 MTR_7g056090 37.132 272 163 5 9 279 10 274 7.43e-57 186
MS.gene34954.t1 MTR_7g084770 37.282 287 166 6 9 290 10 287 1.34e-56 186
MS.gene34954.t1 MTR_6g033055 35.640 289 174 7 7 291 8 288 4.80e-56 184
MS.gene34954.t1 MTR_4g128570 33.904 292 173 4 1 291 1 273 5.30e-56 183
MS.gene34954.t1 MTR_7g093530 34.694 294 177 5 1 292 1 281 6.99e-56 183
MS.gene34954.t1 MTR_4g126920 34.875 281 168 6 15 292 15 283 1.85e-54 180
MS.gene34954.t1 MTR_4g097640 37.282 287 164 8 11 291 12 288 4.61e-54 179
MS.gene34954.t1 MTR_4g097670 37.282 287 164 8 11 291 12 288 4.61e-54 179
MS.gene34954.t1 MTR_4g128400 36.719 256 154 4 39 292 36 285 4.75e-54 179
MS.gene34954.t1 MTR_4g097700 38.235 272 151 9 26 291 65 325 9.06e-53 177
MS.gene34954.t1 MTR_4g128580 35.955 267 159 5 31 291 26 286 1.35e-51 172
MS.gene34954.t1 MTR_3g084630 36.015 261 155 5 31 290 28 277 1.93e-51 172
MS.gene34954.t1 MTR_3g084620 36.015 261 155 5 31 290 28 277 2.31e-51 172
MS.gene34954.t1 MTR_2g038860 34.694 294 166 7 8 291 10 287 1.43e-50 170
MS.gene34954.t1 MTR_2g038705 34.694 294 166 7 8 291 10 287 1.43e-50 170
MS.gene34954.t1 MTR_8g081040 34.733 262 160 4 30 290 30 281 1.08e-49 167
MS.gene34954.t1 MTR_2g089140 38.696 230 125 7 72 291 1 224 1.82e-49 165
MS.gene34954.t1 MTR_2g049130 33.214 280 171 9 1 276 1 268 2.89e-49 166
MS.gene34954.t1 MTR_8g102220 34.256 289 162 7 3 290 5 266 3.25e-49 166
MS.gene34954.t1 MTR_6g088320 32.770 296 175 8 3 290 4 283 9.40e-49 165
MS.gene34954.t1 MTR_2g049030 32.616 279 173 8 1 276 1 267 3.49e-47 160
MS.gene34954.t1 MTR_8g102910 36.179 246 147 5 50 290 39 279 8.10e-47 160
MS.gene34954.t1 MTR_4g128590 32.302 291 163 7 12 290 7 275 9.05e-45 154
MS.gene34954.t1 MTR_8g032640 35.028 177 103 6 52 226 32 198 6.58e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34954.t1 AT1G14720 67.169 332 107 2 1 331 1 331 1.95e-168 472
MS.gene34954.t1 AT2G01850 68.278 331 98 5 6 330 4 333 1.91e-167 469
MS.gene34954.t1 AT1G32170 56.090 312 129 4 2 309 5 312 2.98e-124 360
MS.gene34954.t1 AT4G18990 51.780 309 131 4 25 319 34 338 6.66e-110 324
MS.gene34954.t1 AT1G10550 46.691 272 130 4 30 290 42 309 3.56e-83 254
MS.gene34954.t1 AT4G18990 51.055 237 98 4 97 319 32 264 8.95e-82 250
MS.gene34954.t1 AT4G13090 44.483 290 146 8 11 293 10 291 2.12e-74 231
MS.gene34954.t1 AT4G13080 39.000 300 166 6 4 293 2 294 3.34e-71 223
MS.gene34954.t1 AT3G44990 37.458 299 165 6 6 290 3 293 1.86e-64 206
MS.gene34954.t1 AT3G25050 37.248 298 170 9 1 290 1 289 1.06e-63 204
MS.gene34954.t1 AT1G11545 38.514 296 173 7 4 294 12 303 2.28e-63 203
MS.gene34954.t1 AT5G13870 38.754 289 162 7 6 290 10 287 3.45e-63 202
MS.gene34954.t1 AT5G13870 38.754 289 162 7 6 290 10 287 3.45e-63 202
MS.gene34954.t1 AT5G13870 38.754 289 162 7 6 290 10 287 3.45e-63 202
MS.gene34954.t1 AT2G36870 38.689 305 164 6 1 290 1 297 3.73e-63 202
MS.gene34954.t1 AT2G06850 37.931 290 171 6 3 290 8 290 1.87e-59 193
MS.gene34954.t1 AT4G37800 38.194 288 157 9 9 290 17 289 1.29e-57 188
MS.gene34954.t1 AT4G25810 36.913 298 164 8 1 291 4 284 2.22e-57 187
MS.gene34954.t1 AT5G65730 36.713 286 170 7 6 290 15 290 2.72e-57 187
MS.gene34954.t1 AT5G57550 37.063 286 166 8 10 290 6 282 1.39e-56 185
MS.gene34954.t1 AT2G06850 42.489 233 126 5 60 290 4 230 1.29e-55 181
MS.gene34954.t1 AT5G57560 36.000 300 165 9 1 291 1 282 2.50e-55 182
MS.gene34954.t1 AT2G14620 36.301 292 172 6 6 292 14 296 2.13e-54 180
MS.gene34954.t1 AT4G30270 35.789 285 157 6 9 291 6 266 2.33e-54 179
MS.gene34954.t1 AT4G03210 34.875 281 171 6 14 290 12 284 3.34e-53 177
MS.gene34954.t1 AT3G23730 35.714 266 161 4 30 290 26 286 3.51e-53 177
MS.gene34954.t1 AT4G14130 36.525 282 165 6 11 290 15 284 7.64e-53 176
MS.gene34954.t1 AT5G48070 35.915 284 168 7 10 290 9 281 8.58e-53 175
MS.gene34954.t1 AT4G30290 38.732 284 160 7 10 290 4 276 1.28e-52 175
MS.gene34954.t1 AT4G30280 38.380 284 161 7 10 290 9 281 1.72e-52 174
MS.gene34954.t1 AT5G57530 35.581 267 162 6 30 293 26 285 7.53e-52 173
MS.gene34954.t1 AT5G57540 34.831 267 164 6 30 293 25 284 3.61e-51 171
MS.gene34954.t1 AT2G18800 35.125 279 154 5 31 291 28 297 4.77e-51 171
MS.gene34954.t1 AT1G65310 39.062 256 143 6 39 291 37 282 9.23e-51 170
MS.gene34954.t1 AT1G65310 39.062 256 143 6 39 291 59 304 1.37e-50 170
MS.gene34954.t1 AT4G28850 33.898 295 177 7 6 290 1 287 8.74e-50 168
MS.gene34954.t1 AT4G03210 36.905 252 148 6 44 290 11 256 1.47e-49 166
MS.gene34954.t1 AT2G14620 39.407 236 131 5 62 292 18 246 2.25e-49 166
MS.gene34954.t1 AT2G14620 39.648 227 125 5 71 292 16 235 8.37e-48 161
MS.gene34954.t1 AT4G25820 33.692 279 172 5 16 292 20 287 1.58e-47 162
MS.gene34954.t1 AT3G48580 33.884 242 133 10 53 290 55 273 3.23e-35 129
MS.gene34954.t1 AT3G48580 34.188 234 127 10 61 290 4 214 2.50e-33 123
MS.gene34954.t1 AT2G36870 38.889 180 93 3 1 165 1 178 1.94e-32 119
MS.gene34954.t1 AT3G48580 30.165 242 122 11 53 290 55 253 1.66e-21 92.4

Find 80 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATTCAGACCAATGTTTA+TGG 0.234371 4.4:+17732116 MS.gene34954:CDS
ATCTAGAAATTTACCTATTA+CGG 0.254701 4.4:+17731263 MS.gene34954:CDS
TAGGACAAGGTCAGAGAATT+TGG 0.267570 4.4:-17733203 None:intergenic
TCTCATGATATTTACCTTCA+TGG 0.300530 4.4:+17731499 MS.gene34954:CDS
GTCCGTGTAACTTCTCTAAT+AGG 0.305543 4.4:-17733055 None:intergenic
GAAGGGTACACACCTTTGTT+TGG 0.310448 4.4:+17731294 MS.gene34954:CDS
AAGGGTACACACCTTTGTTT+GGG 0.339045 4.4:+17731295 MS.gene34954:CDS
CCTTCATCAAAGGCCGTAAT+AGG 0.340035 4.4:-17731276 None:intergenic
AAATCAAAATGGAGAACTTC+AGG 0.359114 4.4:+17733320 MS.gene34954:CDS
TCCAGAGAATACTGTATTGA+TGG 0.361854 4.4:-17732205 None:intergenic
ATTCATGGGTTATTCTCATA+TGG 0.374443 4.4:+17731194 None:intergenic
AGTTCCAAATCCATTCCTTC+TGG 0.389842 4.4:+17733280 MS.gene34954:CDS
CTTACTGTTACGACAAAATC+AGG 0.399238 4.4:+17733362 MS.gene34954:CDS
AACACCAGAAGGAATGGATT+TGG 0.411428 4.4:-17733284 None:intergenic
GAAACATTAGAGGCAAAGAT+TGG 0.421060 4.4:+17732093 MS.gene34954:CDS
CCTATTACGGCCTTTGATGA+AGG 0.421584 4.4:+17731276 MS.gene34954:CDS
TTCTCTGACCTTGTCCTACA+TGG 0.444477 4.4:+17733208 MS.gene34954:CDS
CTGCCGCTGCCGAATGTGAC+AGG 0.447195 4.4:-17733454 None:intergenic
CTTAGCCTTTCAGCTTCTTG+TGG 0.450432 4.4:-17733421 None:intergenic
TACAGAGTCATAGGCTTGGC+AGG 0.452062 4.4:-17733100 None:intergenic
TGACTCTGTATGCGACTGTA+TGG 0.452714 4.4:+17733113 MS.gene34954:CDS
CTCATGATATTTACCTTCAT+GGG 0.454019 4.4:+17731500 MS.gene34954:CDS
CTTTCTTTAGATGAAAGAAC+AGG 0.466457 4.4:+17731360 MS.gene34954:CDS
TCTTTCCTCTCTGCCAATGC+TGG 0.468353 4.4:-17732150 None:intergenic
TCCATCAATACAGTATTCTC+TGG 0.470704 4.4:+17732204 MS.gene34954:CDS
TCAGAGAATTTGGCAACATA+TGG 0.479047 4.4:-17733193 None:intergenic
ACAGTCGCATACAGAGTCAT+AGG 0.480696 4.4:-17733109 None:intergenic
CATCAGATTGGGCTACCAAT+GGG 0.480813 4.4:+17733140 MS.gene34954:CDS
AACGTCTTTGTTCATAGAGA+TGG 0.488359 4.4:+17731324 MS.gene34954:CDS
CAGACCAATGTTTATGGCAA+TGG 0.498239 4.4:+17732122 MS.gene34954:CDS
AAATTCGACCCTGTCACATT+CGG 0.498531 4.4:+17733445 MS.gene34954:CDS
GCATCAGATTGGGCTACCAA+TGG 0.503583 4.4:+17733139 MS.gene34954:CDS
GAGAGGAAAGATATGGTCTC+TGG 0.504612 4.4:+17732162 MS.gene34954:CDS
CTGTATGGGATGCATCAGAT+TGG 0.506113 4.4:+17733128 MS.gene34954:CDS
ACTCAAAATCTATTTCATCA+TGG 0.506747 4.4:-17732064 None:intergenic
CACAAGAAGCTGAAAGGCTA+AGG 0.507826 4.4:+17733422 MS.gene34954:CDS
CACTTGGCTACATGCTCGAT+TGG 0.512580 4.4:-17733244 None:intergenic
CTATTACGGCCTTTGATGAA+GGG 0.522479 4.4:+17731277 MS.gene34954:CDS
TGTATGGGATGCATCAGATT+GGG 0.528354 4.4:+17733129 MS.gene34954:CDS
CATAGAAAATAGGAAGAGAA+AGG 0.528369 4.4:-17731217 None:intergenic
TGGTGTAACACCAGAAGGAA+TGG 0.534559 4.4:-17733290 None:intergenic
TATTGATGGAAATCCTCTGC+AGG 0.536591 4.4:-17732191 None:intergenic
GGCAATGGTAGCACCAGCAT+TGG 0.536609 4.4:+17732137 MS.gene34954:CDS
TTGTATCTGCAGATGTCAAA+TGG 0.539892 4.4:+17732023 MS.gene34954:intron
CGCATACAGAGTCATAGGCT+TGG 0.544941 4.4:-17733104 None:intergenic
CGTGGTTTCCTCTGCTGTGG+TGG 0.546130 4.4:-17733498 None:intergenic
TGTTCATAGAGATGGCAAAT+CGG 0.550991 4.4:+17731332 MS.gene34954:CDS
TGTACTGGTGTAACACCAGA+AGG 0.555810 4.4:-17733295 None:intergenic
GACCCTGTCACATTCGGCAG+CGG 0.558184 4.4:+17733451 MS.gene34954:CDS
ACATTAGAGGCAAAGATTGG+AGG 0.561664 4.4:+17732096 MS.gene34954:CDS
GGATTGATCACGCACTCTGA+TGG 0.565213 4.4:-17733400 None:intergenic
AAGCACTGAAATACCCATGA+AGG 0.569254 4.4:-17731513 None:intergenic
ATTTCTAGATGAAGCAGAAG+CGG 0.570082 4.4:-17731250 None:intergenic
ATTGGCAGAGAGGAAAGATA+TGG 0.574281 4.4:+17732155 MS.gene34954:CDS
ACTTTGAGCAGTGTCACACT+TGG 0.575287 4.4:-17733260 None:intergenic
CTGCGTGGTTTCCTCTGCTG+TGG 0.577797 4.4:-17733501 None:intergenic
GACTCTGTATGCGACTGTAT+GGG 0.581043 4.4:+17733114 MS.gene34954:CDS
TTACCTGCTGATTACACTGC+CGG 0.582709 4.4:+17731544 MS.gene34954:CDS
ACACCGGCAGTGTAATCAGC+AGG 0.593919 4.4:-17731547 None:intergenic
AGGTGTGTACCCTTCATCAA+AGG 0.593957 4.4:-17731286 None:intergenic
GCTACCATTGCCATAAACAT+TGG 0.602231 4.4:-17732126 None:intergenic
TCTCTGGTTTGATCCTGCAG+AGG 0.605207 4.4:+17732178 MS.gene34954:CDS
AAACGACACCACCACAGCAG+AGG 0.605813 4.4:+17733490 MS.gene34954:CDS
TCAATCCACAAGAAGCTGAA+AGG 0.606328 4.4:+17733416 MS.gene34954:CDS
AGTTACACGGACAAAATCGA+TGG 0.608259 4.4:+17733066 MS.gene34954:CDS
TCAGATTGGGCTACCAATGG+GGG 0.608759 4.4:+17733142 MS.gene34954:CDS
GATCGACTGCACAACCATGT+AGG 0.610773 4.4:-17733222 None:intergenic
TGCCGCTGCCGAATGTGACA+GGG 0.625854 4.4:-17733453 None:intergenic
ATCAGATTGGGCTACCAATG+GGG 0.630175 4.4:+17733141 MS.gene34954:CDS
TTGATCACGCACTCTGATGG+AGG 0.630279 4.4:-17733397 None:intergenic
ACAGAGTCATAGGCTTGGCA+GGG 0.644912 4.4:-17733099 None:intergenic
GGCAGCGGCAGACATCGTCA+TGG 0.658799 4.4:+17733466 MS.gene34954:CDS
CTGCACAACCATGTAGGACA+AGG 0.660708 4.4:-17733216 None:intergenic
AAAATGAAACAGCTTCTGCG+TGG 0.667909 4.4:-17733516 None:intergenic
GTTACACGGACAAAATCGAT+GGG 0.671108 4.4:+17733067 MS.gene34954:CDS
TTCCTATTAGAGAAGTTACA+CGG 0.672955 4.4:+17733053 MS.gene34954:CDS
TAGCACCAGCATTGGCAGAG+AGG 0.673176 4.4:+17732145 MS.gene34954:CDS
AGGGTACACACCTTTGTTTG+GGG 0.683056 4.4:+17731296 MS.gene34954:CDS
ACGTTATTATCCCCAAACAA+AGG 0.684297 4.4:-17731306 None:intergenic
ACACGGACAAAATCGATGGG+AGG 0.707346 4.4:+17733070 MS.gene34954:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATCCAATAAAAGTAAA+AGG + chr4.4:17731979-17731998 MS.gene34954:intron 15.0%
!! AAAAATTAAGAGACAAAAAT+AGG - chr4.4:17731667-17731686 None:intergenic 15.0%
!!! ATTATTCCTTTTACTTTTAT+TGG - chr4.4:17731988-17732007 None:intergenic 15.0%
!! ATCTAGAAATTTACCTATTA+CGG + chr4.4:17731263-17731282 MS.gene34954:CDS 20.0%
!! GAAAAAAAATATTGCATGAA+AGG - chr4.4:17732374-17732393 None:intergenic 20.0%
!! TAGTATTACTAAGAAAATGT+AGG - chr4.4:17732324-17732343 None:intergenic 20.0%
!!! AAAGAAAAAGCATAGAAAAT+AGG - chr4.4:17731230-17731249 None:intergenic 20.0%
!!! CAATAAAGATCTTTGTTTAT+AGG + chr4.4:17732413-17732432 MS.gene34954:intron 20.0%
!!! GAAATAGATTTTGAGTTTTT+GGG + chr4.4:17732071-17732090 MS.gene34954:CDS 20.0%
!!! GGATAAAGTATTAATCTTTT+TGG + chr4.4:17731434-17731453 MS.gene34954:intron 20.0%
!!! TGAAATAGATTTTGAGTTTT+TGG + chr4.4:17732070-17732089 MS.gene34954:CDS 20.0%
! AAGGAATCTATATATGACTT+GGG - chr4.4:17732520-17732539 None:intergenic 25.0%
! ACCAGTACAAAAAATCAAAA+TGG + chr4.4:17733309-17733328 MS.gene34954:CDS 25.0%
! ACTCAAAATCTATTTCATCA+TGG - chr4.4:17732067-17732086 None:intergenic 25.0%
! AGATGCAAGTAAATATTTGT+CGG - chr4.4:17732791-17732810 None:intergenic 25.0%
! ATTTAGTTGTTTATCCAAAC+AGG - chr4.4:17732572-17732591 None:intergenic 25.0%
! GAAAACAGGAATACAATATT+AGG - chr4.4:17733006-17733025 None:intergenic 25.0%
! GAAAATGTAGGTATATTCTT+TGG - chr4.4:17732312-17732331 None:intergenic 25.0%
! TCAAAACATCGTAATTAACA+TGG - chr4.4:17732959-17732978 None:intergenic 25.0%
! TTTAGTTGTTTATCCAAACA+GGG - chr4.4:17732571-17732590 None:intergenic 25.0%
!! AATAAGTTGAAATCAGCTTA+TGG + chr4.4:17732726-17732745 MS.gene34954:intron 25.0%
!! TTTGGAATGTAAAAATCCAA+AGG - chr4.4:17732294-17732313 None:intergenic 25.0%
!!! TCCATTTTGATTTTTTGTAC+TGG - chr4.4:17733313-17733332 None:intergenic 25.0%
!!! TTTTTGGTTTTTCTGTTGTT+GGG + chr4.4:17731450-17731469 MS.gene34954:intron 25.0%
AAATCAAAATGGAGAACTTC+AGG + chr4.4:17733320-17733339 MS.gene34954:CDS 30.0%
ACTGATTGTGACTAAAACTT+AGG + chr4.4:17731868-17731887 MS.gene34954:intron 30.0%
ATAAAAAGCAACAACAACAC+CGG - chr4.4:17731566-17731585 None:intergenic 30.0%
ATCTACATAAAATCTGCACA+AGG - chr4.4:17733029-17733048 None:intergenic 30.0%
CAAGGAATCTATATATGACT+TGG - chr4.4:17732521-17732540 None:intergenic 30.0%
CATAGAAAATAGGAAGAGAA+AGG - chr4.4:17731220-17731239 None:intergenic 30.0%
CTCATGATATTTACCTTCAT+GGG + chr4.4:17731500-17731519 MS.gene34954:CDS 30.0%
CTTGAGAATATAATCCGATA+AGG + chr4.4:17731710-17731729 MS.gene34954:intron 30.0%
CTTTCTTTAGATGAAAGAAC+AGG + chr4.4:17731360-17731379 MS.gene34954:CDS 30.0%
GATGCAAGTAAATATTTGTC+GGG - chr4.4:17732790-17732809 None:intergenic 30.0%
GGCTTATAATCAAATCATCT+TGG + chr4.4:17732913-17732932 MS.gene34954:intron 30.0%
TATTCAGCTGTTGAAATTCA+AGG + chr4.4:17731753-17731772 MS.gene34954:intron 30.0%
TCTACATAAAATCTGCACAA+GGG - chr4.4:17733028-17733047 None:intergenic 30.0%
TCTCATGATATTTACCTTCA+TGG + chr4.4:17731499-17731518 MS.gene34954:CDS 30.0%
TGAAGATGCTTAAAATCTGT+CGG + chr4.4:17732823-17732842 MS.gene34954:intron 30.0%
TTCCTATTAGAGAAGTTACA+CGG + chr4.4:17733053-17733072 MS.gene34954:CDS 30.0%
TTGATGTTACTATGATCTTG+TGG + chr4.4:17731413-17731432 MS.gene34954:intron 30.0%
!!! CTTTTTGGTTTTTCTGTTGT+TGG + chr4.4:17731449-17731468 MS.gene34954:intron 30.0%
AACAGGTTTGAAAATCTGCA+TGG + chr4.4:17731377-17731396 MS.gene34954:intron 35.0%
AACCAGATATGGTATGTATG+AGG - chr4.4:17732269-17732288 None:intergenic 35.0%
AACCTCATACATACCATATC+TGG + chr4.4:17732264-17732283 MS.gene34954:intron 35.0%
AACGTCTTTGTTCATAGAGA+TGG + chr4.4:17731324-17731343 MS.gene34954:CDS 35.0%
AATTTCAACAGTGACGTTTC+TGG - chr4.4:17731933-17731952 None:intergenic 35.0%
ACAGGTTTGAAAATCTGCAT+GGG + chr4.4:17731378-17731397 MS.gene34954:intron 35.0%
ACGTTATTATCCCCAAACAA+AGG - chr4.4:17731309-17731328 None:intergenic 35.0%
AGGATTCAGACCAATGTTTA+TGG + chr4.4:17732116-17732135 MS.gene34954:CDS 35.0%
ATGTTTGATCAGTTGACAGT+AGG + chr4.4:17732868-17732887 MS.gene34954:intron 35.0%
CAACTGATCAAACATTCACA+CGG - chr4.4:17732863-17732882 None:intergenic 35.0%
CTTACTGTTACGACAAAATC+AGG + chr4.4:17733362-17733381 MS.gene34954:CDS 35.0%
GAAACATTAGAGGCAAAGAT+TGG + chr4.4:17732093-17732112 MS.gene34954:CDS 35.0%
TAAATATTTGTCGGGCTATC+TGG - chr4.4:17732782-17732801 None:intergenic 35.0%
TCAGAGAATTTGGCAACATA+TGG - chr4.4:17733196-17733215 None:intergenic 35.0%
TCCAGAGAATACTGTATTGA+TGG - chr4.4:17732208-17732227 None:intergenic 35.0%
TCCATCAATACAGTATTCTC+TGG + chr4.4:17732204-17732223 MS.gene34954:CDS 35.0%
TGTTCATAGAGATGGCAAAT+CGG + chr4.4:17731332-17731351 MS.gene34954:CDS 35.0%
TGTTTGATCAGTTGACAGTA+GGG + chr4.4:17732869-17732888 MS.gene34954:intron 35.0%
TTGTATCTGCAGATGTCAAA+TGG + chr4.4:17732023-17732042 MS.gene34954:intron 35.0%
! ATTTCTAGATGAAGCAGAAG+CGG - chr4.4:17731253-17731272 None:intergenic 35.0%
!! GAGTTTTTGGGAAACATTAG+AGG + chr4.4:17732083-17732102 MS.gene34954:CDS 35.0%
!!! ATCTAGCTGTTTTTTGCTGT+TGG + chr4.4:17732446-17732465 MS.gene34954:intron 35.0%
!!! TCTAGCTGTTTTTTGCTGTT+GGG + chr4.4:17732447-17732466 MS.gene34954:intron 35.0%
AAAATGAAACAGCTTCTGCG+TGG - chr4.4:17733519-17733538 None:intergenic 40.0%
AAATCTGCACAAGGGAAAAC+AGG - chr4.4:17733020-17733039 None:intergenic 40.0%
AAATTCGACCCTGTCACATT+CGG + chr4.4:17733445-17733464 MS.gene34954:CDS 40.0%
ACATTAGAGGCAAAGATTGG+AGG + chr4.4:17732096-17732115 MS.gene34954:CDS 40.0%
AGTTACACGGACAAAATCGA+TGG + chr4.4:17733066-17733085 MS.gene34954:CDS 40.0%
AGTTCCAAATCCATTCCTTC+TGG + chr4.4:17733280-17733299 MS.gene34954:CDS 40.0%
ATCCAAAGGTGAACCAGATA+TGG - chr4.4:17732280-17732299 None:intergenic 40.0%
ATTGGCAGAGAGGAAAGATA+TGG + chr4.4:17732155-17732174 MS.gene34954:CDS 40.0%
ATTTGATTATAAGCCCCCGA+TGG - chr4.4:17732908-17732927 None:intergenic 40.0%
CAGACCAATGTTTATGGCAA+TGG + chr4.4:17732122-17732141 MS.gene34954:CDS 40.0%
CTATTACGGCCTTTGATGAA+GGG + chr4.4:17731277-17731296 MS.gene34954:CDS 40.0%
GCTACCATTGCCATAAACAT+TGG - chr4.4:17732129-17732148 None:intergenic 40.0%
GTCCGTGTAACTTCTCTAAT+AGG - chr4.4:17733058-17733077 None:intergenic 40.0%
GTTACACGGACAAAATCGAT+GGG + chr4.4:17733067-17733086 MS.gene34954:CDS 40.0%
TAGGACAAGGTCAGAGAATT+TGG - chr4.4:17733206-17733225 None:intergenic 40.0%
TATTGATGGAAATCCTCTGC+AGG - chr4.4:17732194-17732213 None:intergenic 40.0%
TCAATCCACAAGAAGCTGAA+AGG + chr4.4:17733416-17733435 MS.gene34954:CDS 40.0%
TGTATGGGATGCATCAGATT+GGG + chr4.4:17733129-17733148 MS.gene34954:CDS 40.0%
! AACACCAGAAGGAATGGATT+TGG - chr4.4:17733287-17733306 None:intergenic 40.0%
! AAGGGTACACACCTTTGTTT+GGG + chr4.4:17731295-17731314 MS.gene34954:CDS 40.0%
! TACCATATCTGGTTCACCTT+TGG + chr4.4:17732275-17732294 MS.gene34954:intron 40.0%
!! AAGCACTGAAATACCCATGA+AGG - chr4.4:17731516-17731535 None:intergenic 40.0%
!!! GTTTTTTGCTGTTGGGTTTG+TGG + chr4.4:17732454-17732473 MS.gene34954:intron 40.0%
AACAGGGCAAATCGAATGTG+TGG - chr4.4:17732555-17732574 None:intergenic 45.0%
AACTCTGTACTTTCCCCCAT+TGG - chr4.4:17733158-17733177 None:intergenic 45.0%
AATCCATATCCTACCATCGG+GGG + chr4.4:17732892-17732911 MS.gene34954:intron 45.0%
ACAGTCGCATACAGAGTCAT+AGG - chr4.4:17733112-17733131 None:intergenic 45.0%
ACTTTGAGCAGTGTCACACT+TGG - chr4.4:17733263-17733282 None:intergenic 45.0%
AGGTGTGTACCCTTCATCAA+AGG - chr4.4:17731289-17731308 None:intergenic 45.0%
AGTCACAATCAGTGAGCATG+CGG - chr4.4:17731861-17731880 None:intergenic 45.0%
ATCAGATTGGGCTACCAATG+GGG + chr4.4:17733141-17733160 MS.gene34954:CDS 45.0%
CACAAGAAGCTGAAAGGCTA+AGG + chr4.4:17733422-17733441 MS.gene34954:CDS 45.0%
CATCAGATTGGGCTACCAAT+GGG + chr4.4:17733140-17733159 MS.gene34954:CDS 45.0%
CCTATTACGGCCTTTGATGA+AGG + chr4.4:17731276-17731295 MS.gene34954:CDS 45.0%
CCTTCATCAAAGGCCGTAAT+AGG - chr4.4:17731279-17731298 None:intergenic 45.0%
CTGTATGGGATGCATCAGAT+TGG + chr4.4:17733128-17733147 MS.gene34954:CDS 45.0%
GAATCCATATCCTACCATCG+GGG + chr4.4:17732891-17732910 MS.gene34954:intron 45.0%
GACTCTGTATGCGACTGTAT+GGG + chr4.4:17733114-17733133 MS.gene34954:CDS 45.0%
GAGAGGAAAGATATGGTCTC+TGG + chr4.4:17732162-17732181 MS.gene34954:CDS 45.0%
GGAATCCATATCCTACCATC+GGG + chr4.4:17732890-17732909 MS.gene34954:intron 45.0%
GGGAATCCATATCCTACCAT+CGG + chr4.4:17732889-17732908 MS.gene34954:intron 45.0%
TGACTCTGTATGCGACTGTA+TGG + chr4.4:17733113-17733132 MS.gene34954:CDS 45.0%
TGGTGTAACACCAGAAGGAA+TGG - chr4.4:17733293-17733312 None:intergenic 45.0%
TTCTCTGACCTTGTCCTACA+TGG + chr4.4:17733208-17733227 MS.gene34954:CDS 45.0%
! AGGGTACACACCTTTGTTTG+GGG + chr4.4:17731296-17731315 MS.gene34954:CDS 45.0%
! CACATTCGATTTGCCCTGTT+TGG + chr4.4:17732555-17732574 MS.gene34954:intron 45.0%
! CTTAGCCTTTCAGCTTCTTG+TGG - chr4.4:17733424-17733443 None:intergenic 45.0%
! GAAGGGTACACACCTTTGTT+TGG + chr4.4:17731294-17731313 MS.gene34954:CDS 45.0%
! TGTACTGGTGTAACACCAGA+AGG - chr4.4:17733298-17733317 None:intergenic 45.0%
! TGTTGTTGGGTTGTGTTTGC+AGG + chr4.4:17731463-17731482 MS.gene34954:intron 45.0%
! TTACCTGCTGATTACACTGC+CGG + chr4.4:17731544-17731563 MS.gene34954:CDS 45.0%
! TTCAACAGTGACGTTTCTGG+TGG - chr4.4:17731930-17731949 None:intergenic 45.0%
AATCAGTGAGCATGCGGTTG+AGG - chr4.4:17731855-17731874 None:intergenic 50.0%
ACACGGACAAAATCGATGGG+AGG + chr4.4:17733070-17733089 MS.gene34954:CDS 50.0%
ACAGAGTCATAGGCTTGGCA+GGG - chr4.4:17733102-17733121 None:intergenic 50.0%
CACTTGGCTACATGCTCGAT+TGG - chr4.4:17733247-17733266 None:intergenic 50.0%
CCCTGAGACTGAGACCTTAT+CGG - chr4.4:17731727-17731746 None:intergenic 50.0%
CCGATAAGGTCTCAGTCTCA+GGG + chr4.4:17731724-17731743 MS.gene34954:intron 50.0%
CGCATACAGAGTCATAGGCT+TGG - chr4.4:17733107-17733126 None:intergenic 50.0%
CTGCACAACCATGTAGGACA+AGG - chr4.4:17733219-17733238 None:intergenic 50.0%
GATCGACTGCACAACCATGT+AGG - chr4.4:17733225-17733244 None:intergenic 50.0%
GATTATAAGCCCCCGATGGT+AGG - chr4.4:17732904-17732923 None:intergenic 50.0%
GCATCAGATTGGGCTACCAA+TGG + chr4.4:17733139-17733158 MS.gene34954:CDS 50.0%
GGATTGATCACGCACTCTGA+TGG - chr4.4:17733403-17733422 None:intergenic 50.0%
TACAGAGTCATAGGCTTGGC+AGG - chr4.4:17733103-17733122 None:intergenic 50.0%
TCAGATTGGGCTACCAATGG+GGG + chr4.4:17733142-17733161 MS.gene34954:CDS 50.0%
TCCGATAAGGTCTCAGTCTC+AGG + chr4.4:17731723-17731742 MS.gene34954:intron 50.0%
TCTTTCCTCTCTGCCAATGC+TGG - chr4.4:17732153-17732172 None:intergenic 50.0%
TGGGTTGTGTTTGCAGGTTC+TGG + chr4.4:17731469-17731488 MS.gene34954:intron 50.0%
TGTGTGGTCTCTTGACAGCA+AGG - chr4.4:17732539-17732558 None:intergenic 50.0%
TTGATCACGCACTCTGATGG+AGG - chr4.4:17733400-17733419 None:intergenic 50.0%
! TCTCTGGTTTGATCCTGCAG+AGG + chr4.4:17732178-17732197 MS.gene34954:CDS 50.0%
AAACGACACCACCACAGCAG+AGG + chr4.4:17733490-17733509 MS.gene34954:CDS 55.0%
AAGCCCCCGATGGTAGGATA+TGG - chr4.4:17732898-17732917 None:intergenic 55.0%
ACACCGGCAGTGTAATCAGC+AGG - chr4.4:17731550-17731569 None:intergenic 55.0%
GGCAATGGTAGCACCAGCAT+TGG + chr4.4:17732137-17732156 MS.gene34954:CDS 55.0%
!! TAGCACCAGCATTGGCAGAG+AGG + chr4.4:17732145-17732164 MS.gene34954:CDS 55.0%
CGTGGTTTCCTCTGCTGTGG+TGG - chr4.4:17733501-17733520 None:intergenic 60.0%
CTGCGTGGTTTCCTCTGCTG+TGG - chr4.4:17733504-17733523 None:intergenic 60.0%
GACCCTGTCACATTCGGCAG+CGG + chr4.4:17733451-17733470 MS.gene34954:CDS 60.0%
TGCCGCTGCCGAATGTGACA+GGG - chr4.4:17733456-17733475 None:intergenic 60.0%
CTGCCGCTGCCGAATGTGAC+AGG - chr4.4:17733457-17733476 None:intergenic 65.0%
GGCAGCGGCAGACATCGTCA+TGG + chr4.4:17733466-17733485 MS.gene34954:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 17731198 17733540 17731198 ID=MS.gene34954
chr4.4 mRNA 17731198 17733540 17731198 ID=MS.gene34954.t1;Parent=MS.gene34954
chr4.4 exon 17731198 17731381 17731198 ID=MS.gene34954.t1.exon1;Parent=MS.gene34954.t1
chr4.4 CDS 17731198 17731381 17731198 ID=cds.MS.gene34954.t1;Parent=MS.gene34954.t1
chr4.4 exon 17731485 17731585 17731485 ID=MS.gene34954.t1.exon2;Parent=MS.gene34954.t1
chr4.4 CDS 17731485 17731585 17731485 ID=cds.MS.gene34954.t1;Parent=MS.gene34954.t1
chr4.4 exon 17732035 17732243 17732035 ID=MS.gene34954.t1.exon3;Parent=MS.gene34954.t1
chr4.4 CDS 17732035 17732243 17732035 ID=cds.MS.gene34954.t1;Parent=MS.gene34954.t1
chr4.4 exon 17733036 17733540 17733036 ID=MS.gene34954.t1.exon4;Parent=MS.gene34954.t1
chr4.4 CDS 17733036 17733540 17733036 ID=cds.MS.gene34954.t1;Parent=MS.gene34954.t1
Gene Sequence

>MS.gene34954

ATGGGTTATTCTCATATGGCCTTTCTCTTCCTATTTTCTATGCTTTTTCTTTCCGCTTCTGCTTCATCTAGAAATTTACCTATTACGGCCTTTGATGAAGGGTACACACCTTTGTTTGGGGATAATAACGTCTTTGTTCATAGAGATGGCAAATCGGTGCATCTTTCTTTAGATGAAAGAACAGGTTTGAAAATCTGCATGGGTTGTTTTGATTTTTGATGTTACTATGATCTTGTGGATAAAGTATTAATCTTTTTGGTTTTTCTGTTGTTGGGTTGTGTTTGCAGGTTCTGGATTTGTGTCTCATGATATTTACCTTCATGGGTATTTCAGTGCTTCAATTAAGTTACCTGCTGATTACACTGCCGGTGTTGTTGTTGCTTTTTATGTGAGTGATTACTTCTAATTGTTCTTTAATTACTAAATCTGTTTATGAAGCTTTTTTGTTTTTTATATTGATTTTCTTCCTATTTTTGTCTCTTAATTTTTTTGTTTATTTTCTAAGTTTTAAACTTGAGAATATAATCCGATAAGGTCTCAGTCTCAGGGTTATAATATTCAGCTGTTGAAATTCAAGGCATGCATGTGAATTTTTATTAATTTTTTTTTTTTTTGTAAAATAATAATAAATTAGACAGTTTTCAATTTCATTTTCCTCAACCGCATGCTCACTGATTGTGACTAAAACTTAGGATTTTATACAAATTTTTAAATAATTTAATCTAGTTTCCACCAGAAACGTCACTGTTGAAATTCAATCTCTGTTTTATAAAAATTTATAAAAAATCCAATAAAAGTAAAAGGAATAATGTTTCATCATATGTTTTGTATCTGCAGATGTCAAATGGTGACATGTATGAGAAGAACCATGATGAAATAGATTTTGAGTTTTTGGGAAACATTAGAGGCAAAGATTGGAGGATTCAGACCAATGTTTATGGCAATGGTAGCACCAGCATTGGCAGAGAGGAAAGATATGGTCTCTGGTTTGATCCTGCAGAGGATTTCCATCAATACAGTATTCTCTGGACAGATTCTCGTATCATGTAAGCTTCTATCTTTCTTCAACCTCATACATACCATATCTGGTTCACCTTTGGATTTTTACATTCCAAAGAATATACCTACATTTTCTTAGTAATACTAATTTTTTTTTCATCATTTCACTTTTTACCTTTCATGCAATATTTTTTTTCTTCACTTTTGTCTTTCATGCAATAAAGATCTTTGTTTATAGGTAGTAACTTAATCTAGCTGTTTTTTGCTGTTGGGTTTGTGGTGCTTTGTTCAAAGATTTCTCTGATTTTTTCACTTCTTTTCCCAAGTCATATATAGATTCCTTGCTGTCAAGAGACCACACATTCGATTTGCCCTGTTTGGATAAACAACTAAATTAAGCATGCTTATAACATAAACACTTAACATATACAATGCTTATATCTAAGCTATTTCTATAACAAAAGATAAAGCAAAGTCAAAAGATTTTCGAATAAGCTATTTCTAGCTGTTTTCATAAGTTGCTTACGAAAATAAGTTGAAATCAGCTTATGGACATGTCATAAGTTGTTCACATAAGCTCTTCCAGATAGCCCGACAAATATTTACTTGCATCTTTGTTTTGAAATTGAAGATGCTTAAAATCTGTCGGCGTTAATTCACTTACCGTGTGAATGTTTGATCAGTTGACAGTAGGGAATCCATATCCTACCATCGGGGGCTTATAATCAAATCATCTTGGATTTTGTTTTTCATAGTTAACCATGTTAATTACGATGTTTTGATGTTCATAACTATTTACATATTTTCCTAATATTGTATTCCTGTTTTCCCTTGTGCAGATTTTATGTAGATAACGTTCCTATTAGAGAAGTTACACGGACAAAATCGATGGGAGGAGATTTCCCTGCCAAGCCTATGACTCTGTATGCGACTGTATGGGATGCATCAGATTGGGCTACCAATGGGGGAAAGTACAGAGTTAACTACAAGTATGCACCATATGTTGCCAAATTCTCTGACCTTGTCCTACATGGTTGTGCAGTCGATCCAATCGAGCATGTAGCCAAGTGTGACACTGCTCAAAGTTCCAAATCCATTCCTTCTGGTGTTACACCAGTACAAAAAATCAAAATGGAGAACTTCAGGAAGAAGTACATGACATACTCTTACTGTTACGACAAAATCAGGTACAAAGCTGCTCCTCCATCAGAGTGCGTGATCAATCCACAAGAAGCTGAAAGGCTAAGGAAATTCGACCCTGTCACATTCGGCAGCGGCAGACATCGTCATGGAAAACGACACCACCACAGCAGAGGAAACCACGCAGAAGCTGTTTCATTTTAA
Protein sequence

>MS.gene34954.t1

MGYSHMAFLFLFSMLFLSASASSRNLPITAFDEGYTPLFGDNNVFVHRDGKSVHLSLDERTGSGFVSHDIYLHGYFSASIKLPADYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIRGKDWRIQTNVYGNGSTSIGREERYGLWFDPAEDFHQYSILWTDSRIIFYVDNVPIREVTRTKSMGGDFPAKPMTLYATVWDASDWATNGGKYRVNYKYAPYVAKFSDLVLHGCAVDPIEHVAKCDTAQSSKSIPSGVTPVQKIKMENFRKKYMTYSYCYDKIRYKAAPPSECVINPQEAERLRKFDPVTFGSGRHRHGKRHHHSRGNHAEAVSF