Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35109.t1 | RHN62819.1 | 95.5 | 404 | 18 | 0 | 1 | 404 | 138 | 541 | 4.80E-223 | 783.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35109.t1 | Q9SVG3 | 65.8 | 409 | 130 | 4 | 1 | 406 | 137 | 538 | 1.6e-155 | 550.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35109.t1 | A0A072UPP4 | 95.5 | 404 | 18 | 0 | 1 | 404 | 138 | 541 | 3.4e-223 | 783.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050815 | MS.gene35109 | 0.804532 | 2.06E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35109.t1 | MTR_4g094515 | 95.545 | 404 | 18 | 0 | 1 | 404 | 138 | 541 | 0.0 | 772 |
| MS.gene35109.t1 | MTR_4g094515 | 71.744 | 407 | 111 | 2 | 1 | 406 | 750 | 1153 | 0.0 | 578 |
| MS.gene35109.t1 | MTR_2g031490 | 70.000 | 410 | 116 | 2 | 1 | 406 | 135 | 541 | 0.0 | 580 |
| MS.gene35109.t1 | MTR_2g031510 | 70.000 | 410 | 116 | 3 | 1 | 406 | 137 | 543 | 0.0 | 570 |
| MS.gene35109.t1 | MTR_2g031590 | 61.800 | 411 | 149 | 4 | 1 | 406 | 128 | 535 | 6.82e-173 | 494 |
| MS.gene35109.t1 | MTR_2g031560 | 59.553 | 403 | 157 | 4 | 1 | 401 | 123 | 521 | 4.35e-171 | 490 |
| MS.gene35109.t1 | MTR_4g094498 | 59.459 | 407 | 158 | 3 | 1 | 406 | 137 | 537 | 1.21e-162 | 468 |
| MS.gene35109.t1 | MTR_4g094418 | 50.244 | 410 | 193 | 5 | 1 | 402 | 28 | 434 | 8.84e-142 | 417 |
| MS.gene35109.t1 | MTR_2g031390 | 50.864 | 405 | 194 | 3 | 1 | 404 | 130 | 530 | 1.01e-141 | 415 |
| MS.gene35109.t1 | MTR_4g094495 | 53.269 | 413 | 183 | 5 | 1 | 406 | 134 | 543 | 7.43e-139 | 407 |
| MS.gene35109.t1 | MTR_4g094488 | 54.146 | 410 | 178 | 5 | 4 | 406 | 134 | 540 | 8.87e-138 | 405 |
| MS.gene35109.t1 | MTR_4g094480 | 53.922 | 408 | 180 | 6 | 4 | 406 | 134 | 538 | 8.13e-136 | 400 |
| MS.gene35109.t1 | MTR_4g094492 | 51.574 | 413 | 189 | 7 | 1 | 406 | 131 | 539 | 3.83e-135 | 398 |
| MS.gene35109.t1 | MTR_4g094478 | 52.228 | 404 | 185 | 5 | 4 | 402 | 134 | 534 | 1.84e-133 | 394 |
| MS.gene35109.t1 | MTR_4g094520 | 50.980 | 408 | 188 | 5 | 1 | 404 | 134 | 533 | 2.09e-131 | 388 |
| MS.gene35109.t1 | MTR_3g009820 | 48.139 | 403 | 200 | 4 | 1 | 401 | 125 | 520 | 1.62e-128 | 380 |
| MS.gene35109.t1 | MTR_3g009880 | 49.380 | 403 | 195 | 4 | 1 | 401 | 123 | 518 | 1.64e-128 | 380 |
| MS.gene35109.t1 | MTR_2g031420 | 52.130 | 399 | 183 | 5 | 4 | 401 | 134 | 525 | 1.72e-128 | 380 |
| MS.gene35109.t1 | MTR_4g091150 | 51.100 | 409 | 185 | 6 | 1 | 401 | 137 | 538 | 2.89e-128 | 380 |
| MS.gene35109.t1 | MTR_3g009760 | 48.139 | 403 | 200 | 4 | 1 | 401 | 125 | 520 | 4.69e-128 | 379 |
| MS.gene35109.t1 | MTR_4g094518 | 47.160 | 405 | 209 | 3 | 1 | 404 | 131 | 531 | 8.26e-126 | 374 |
| MS.gene35109.t1 | MTR_4g088355 | 50.732 | 410 | 193 | 6 | 1 | 406 | 132 | 536 | 2.13e-125 | 373 |
| MS.gene35109.t1 | MTR_4g091190 | 50.852 | 411 | 189 | 8 | 1 | 406 | 129 | 531 | 2.68e-125 | 372 |
| MS.gene35109.t1 | MTR_4g091170 | 47.202 | 411 | 188 | 6 | 1 | 406 | 159 | 545 | 1.55e-124 | 372 |
| MS.gene35109.t1 | MTR_2g031460 | 50.000 | 398 | 191 | 5 | 4 | 400 | 134 | 524 | 7.78e-124 | 369 |
| MS.gene35109.t1 | MTR_6g017205 | 51.232 | 406 | 189 | 6 | 1 | 402 | 125 | 525 | 1.75e-122 | 365 |
| MS.gene35109.t1 | MTR_4g057990 | 50.493 | 406 | 191 | 5 | 1 | 403 | 124 | 522 | 2.81e-122 | 364 |
| MS.gene35109.t1 | MTR_4g091200 | 49.140 | 407 | 198 | 6 | 1 | 403 | 125 | 526 | 1.12e-119 | 358 |
| MS.gene35109.t1 | MTR_2g023590 | 46.929 | 407 | 203 | 6 | 1 | 402 | 67 | 465 | 4.52e-111 | 334 |
| MS.gene35109.t1 | MTR_1g076830 | 38.462 | 403 | 241 | 4 | 1 | 400 | 134 | 532 | 1.19e-88 | 279 |
| MS.gene35109.t1 | MTR_3g009850 | 48.624 | 218 | 110 | 2 | 186 | 401 | 9 | 226 | 5.09e-68 | 215 |
| MS.gene35109.t1 | MTR_2g031600 | 61.875 | 160 | 61 | 0 | 247 | 406 | 244 | 403 | 2.35e-66 | 217 |
| MS.gene35109.t1 | MTR_2g031600 | 80.172 | 116 | 23 | 0 | 1 | 116 | 129 | 244 | 1.71e-50 | 175 |
| MS.gene35109.t1 | MTR_2g084370 | 44.444 | 108 | 38 | 2 | 1 | 108 | 4 | 89 | 3.71e-24 | 95.9 |
| MS.gene35109.t1 | MTR_2g084405 | 50.000 | 76 | 38 | 0 | 1 | 76 | 4 | 79 | 9.92e-17 | 75.9 |
| MS.gene35109.t1 | MTR_7g117710 | 44.828 | 87 | 42 | 3 | 102 | 187 | 28 | 109 | 7.02e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35109.t1 | AT4G20840 | 65.770 | 409 | 130 | 4 | 1 | 406 | 137 | 538 | 0.0 | 524 |
| MS.gene35109.t1 | AT1G11770 | 62.103 | 409 | 145 | 4 | 1 | 406 | 131 | 532 | 1.36e-174 | 498 |
| MS.gene35109.t1 | AT1G01980 | 63.527 | 414 | 136 | 6 | 1 | 406 | 131 | 537 | 6.80e-174 | 497 |
| MS.gene35109.t1 | AT4G20830 | 64.792 | 409 | 134 | 4 | 1 | 406 | 138 | 539 | 7.30e-172 | 491 |
| MS.gene35109.t1 | AT4G20830 | 64.938 | 405 | 132 | 4 | 1 | 402 | 138 | 535 | 2.42e-170 | 489 |
| MS.gene35109.t1 | AT1G30740 | 59.360 | 406 | 153 | 5 | 4 | 404 | 131 | 529 | 5.23e-155 | 448 |
| MS.gene35109.t1 | AT1G30740 | 59.360 | 406 | 153 | 5 | 4 | 404 | 134 | 532 | 6.43e-155 | 448 |
| MS.gene35109.t1 | AT1G30700 | 55.665 | 406 | 172 | 4 | 1 | 404 | 128 | 527 | 8.76e-146 | 424 |
| MS.gene35109.t1 | AT1G34575 | 52.812 | 409 | 180 | 5 | 1 | 406 | 129 | 527 | 2.83e-139 | 408 |
| MS.gene35109.t1 | AT1G30710 | 52.723 | 404 | 179 | 4 | 1 | 401 | 132 | 526 | 7.67e-138 | 404 |
| MS.gene35109.t1 | AT1G26380 | 52.605 | 403 | 182 | 7 | 1 | 401 | 128 | 523 | 3.47e-133 | 393 |
| MS.gene35109.t1 | AT1G30730 | 50.864 | 405 | 191 | 5 | 1 | 403 | 127 | 525 | 4.85e-131 | 387 |
| MS.gene35109.t1 | AT1G30720 | 51.852 | 405 | 187 | 5 | 1 | 403 | 128 | 526 | 5.76e-131 | 387 |
| MS.gene35109.t1 | AT1G30760 | 50.995 | 402 | 188 | 4 | 3 | 403 | 139 | 532 | 1.74e-129 | 383 |
| MS.gene35109.t1 | AT1G30760 | 51.122 | 401 | 187 | 4 | 3 | 402 | 157 | 549 | 3.21e-129 | 383 |
| MS.gene35109.t1 | AT1G26420 | 50.614 | 407 | 184 | 7 | 1 | 401 | 128 | 523 | 9.20e-129 | 381 |
| MS.gene35109.t1 | AT2G34790 | 48.130 | 401 | 199 | 4 | 3 | 402 | 137 | 529 | 1.35e-127 | 378 |
| MS.gene35109.t1 | AT1G26400 | 49.875 | 401 | 193 | 6 | 1 | 400 | 128 | 521 | 4.61e-126 | 374 |
| MS.gene35109.t1 | AT1G26390 | 49.505 | 404 | 195 | 5 | 1 | 401 | 128 | 525 | 4.46e-125 | 372 |
| MS.gene35109.t1 | AT4G20800 | 49.507 | 406 | 191 | 7 | 1 | 401 | 127 | 523 | 1.91e-122 | 365 |
| MS.gene35109.t1 | AT1G26410 | 49.136 | 405 | 193 | 7 | 1 | 401 | 152 | 547 | 7.29e-122 | 364 |
| MS.gene35109.t1 | AT2G34810 | 48.529 | 408 | 199 | 6 | 1 | 401 | 134 | 537 | 6.20e-121 | 362 |
| MS.gene35109.t1 | AT5G44360 | 49.123 | 399 | 191 | 6 | 5 | 401 | 140 | 528 | 6.56e-120 | 358 |
| MS.gene35109.t1 | AT5G44360 | 49.123 | 399 | 191 | 6 | 5 | 401 | 156 | 544 | 8.06e-120 | 359 |
| MS.gene35109.t1 | AT5G44390 | 47.805 | 410 | 197 | 8 | 3 | 404 | 141 | 541 | 9.85e-120 | 358 |
| MS.gene35109.t1 | AT5G44400 | 46.192 | 407 | 200 | 7 | 3 | 401 | 139 | 534 | 2.88e-119 | 357 |
| MS.gene35109.t1 | AT4G20860 | 45.771 | 402 | 211 | 3 | 1 | 401 | 132 | 527 | 4.62e-119 | 357 |
| MS.gene35109.t1 | AT4G20820 | 45.137 | 401 | 203 | 6 | 4 | 401 | 138 | 524 | 7.12e-113 | 341 |
| MS.gene35109.t1 | AT5G44380 | 46.602 | 412 | 197 | 8 | 3 | 404 | 142 | 540 | 6.84e-109 | 331 |
| MS.gene35109.t1 | AT5G44380 | 46.602 | 412 | 197 | 8 | 3 | 404 | 182 | 580 | 2.38e-108 | 330 |
| MS.gene35109.t1 | AT5G44440 | 43.243 | 407 | 210 | 7 | 4 | 401 | 133 | 527 | 4.22e-106 | 323 |
| MS.gene35109.t1 | AT5G44440 | 43.276 | 409 | 207 | 7 | 4 | 401 | 203 | 597 | 4.01e-105 | 323 |
| MS.gene35109.t1 | AT5G44410 | 44.254 | 409 | 199 | 9 | 4 | 401 | 137 | 527 | 2.40e-103 | 316 |
Find 96 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATATTGGTACTAATGATTT+TGG | 0.132509 | 4.4:-19867619 | MS.gene35109:CDS |
| AAAGGCTCTTCTTGGATTAT+TGG | 0.143770 | 4.4:+19867660 | None:intergenic |
| TATGAACAAGGAGTTGTTTA+TGG | 0.147533 | 4.4:-19867586 | MS.gene35109:CDS |
| GTGAAGTTTATTATAGGATA+TGG | 0.184330 | 4.4:-19868599 | MS.gene35109:CDS |
| ACTGTTATGGCTTTGTTTCT+TGG | 0.219607 | 4.4:-19868132 | MS.gene35109:CDS |
| GGTAATATGATGAGAAAATT+TGG | 0.247418 | 4.4:-19868495 | MS.gene35109:CDS |
| GTGAGTTCTAAGGTTGTAAA+AGG | 0.251251 | 4.4:-19868174 | MS.gene35109:CDS |
| GCTGAAATTGTTGATGTTAA+TGG | 0.253859 | 4.4:-19868447 | MS.gene35109:CDS |
| TTCTTGACATGTTTCAATTC+AGG | 0.256050 | 4.4:-19868680 | None:intergenic |
| GGAGGTGGAGGAGCGAGTTT+TGG | 0.259984 | 4.4:-19868372 | MS.gene35109:CDS |
| AATTATAGGGACCTTGATAT+TGG | 0.291958 | 4.4:-19867634 | MS.gene35109:CDS |
| GATGAACTTGTTACACTATT+GGG | 0.296098 | 4.4:-19868102 | MS.gene35109:CDS |
| AGATTCAGTATTCGGTGAAT+TGG | 0.298312 | 4.4:-19867762 | MS.gene35109:CDS |
| TATTCAGCCTGTGAGTTCTA+AGG | 0.312395 | 4.4:-19868184 | MS.gene35109:CDS |
| TCTATTCAAGATTCAGTATT+CGG | 0.325898 | 4.4:-19867770 | MS.gene35109:CDS |
| TACTTGGTGAAGTTTATTAT+AGG | 0.327921 | 4.4:-19868605 | MS.gene35109:CDS |
| AGGATGGGTTAGAAGGGATA+TGG | 0.329384 | 4.4:-19867900 | MS.gene35109:CDS |
| TGCGGAGAGTTTGGATAGTT+TGG | 0.331621 | 4.4:-19868286 | MS.gene35109:CDS |
| AAAGTTCATGGCTTTCCTGC+TGG | 0.332436 | 4.4:-19868567 | MS.gene35109:CDS |
| ATGGAAGAAGGTGATTGAAT+TGG | 0.341486 | 4.4:-19867881 | MS.gene35109:CDS |
| TTGATCCAGAAAATTTCTTC+AGG | 0.346412 | 4.4:-19867510 | MS.gene35109:CDS |
| TCCATCCTCTATTACTTACT+AGG | 0.368689 | 4.4:+19867463 | None:intergenic |
| ATATCAAAGGATGGGTTAGA+AGG | 0.386312 | 4.4:-19867907 | MS.gene35109:CDS |
| GTTGGATTGGTTCTGTGCTT+TGG | 0.399417 | 4.4:-19868029 | MS.gene35109:CDS |
| AAGGTCCCTATAATTTAAAA+AGG | 0.400774 | 4.4:+19867642 | None:intergenic |
| AAGTTCATGGCTTTCCTGCT+GGG | 0.404875 | 4.4:-19868566 | MS.gene35109:CDS |
| GATTCAGTATTCGGTGAATT+GGG | 0.410302 | 4.4:-19867761 | MS.gene35109:CDS |
| TGGAAGAAGGTGATTGAATT+GGG | 0.414864 | 4.4:-19867880 | MS.gene35109:CDS |
| AAAATTGTTCTGAAATGAGT+TGG | 0.420074 | 4.4:-19868047 | MS.gene35109:CDS |
| GTGATTGAATTGGGAAAAGT+AGG | 0.420297 | 4.4:-19867871 | MS.gene35109:CDS |
| TGAAGTTTATTATAGGATAT+GGG | 0.420618 | 4.4:-19868598 | MS.gene35109:CDS |
| TGATGAACTTGTTACACTAT+TGG | 0.426232 | 4.4:-19868103 | MS.gene35109:CDS |
| TTCCTGTCAAGAAGCTCATC+TGG | 0.428788 | 4.4:+19867982 | None:intergenic |
| TTGCCCCACATACCGATGAT+AGG | 0.429466 | 4.4:-19868224 | MS.gene35109:CDS |
| CTTGGATTATTGGAAACAAA+AGG | 0.430415 | 4.4:+19867670 | None:intergenic |
| GTTTGTCCTACTGTTGGTGT+TGG | 0.433697 | 4.4:-19868543 | MS.gene35109:CDS |
| ACCGCGTTTCCTCACCGTGC+AGG | 0.433754 | 4.4:-19867796 | MS.gene35109:CDS |
| CACTTGAGTGGTGGAGGGTA+TGG | 0.435822 | 4.4:-19868516 | MS.gene35109:CDS |
| GTGCTTTGGTGGGCTAGTAT+TGG | 0.440966 | 4.4:-19868015 | MS.gene35109:CDS |
| TGTCCTACTGTTGGTGTTGG+TGG | 0.450065 | 4.4:-19868540 | MS.gene35109:CDS |
| GTTATGGCTTTGTTTCTTGG+AGG | 0.454128 | 4.4:-19868129 | MS.gene35109:CDS |
| ATTCTTGATAGGAAATCAAT+GGG | 0.454747 | 4.4:-19868420 | MS.gene35109:CDS |
| TTAATGGTAGGATTCTTGAT+AGG | 0.456529 | 4.4:-19868431 | MS.gene35109:CDS |
| GATTGGTTCTGTGCTTTGGT+GGG | 0.458831 | 4.4:-19868025 | MS.gene35109:CDS |
| GGTGGTCACTTGAGTGGTGG+AGG | 0.467181 | 4.4:-19868522 | MS.gene35109:CDS |
| TTTATCATACAGTATTAAGT+TGG | 0.473381 | 4.4:-19868343 | MS.gene35109:CDS |
| TCTTGACAGGAATATTGATT+CGG | 0.475533 | 4.4:-19867971 | MS.gene35109:CDS |
| AACTGTTAGAGTGACTGTTA+TGG | 0.479481 | 4.4:-19868145 | MS.gene35109:CDS |
| TGAATTGGGAAAAGTAGGAT+TGG | 0.485750 | 4.4:-19867866 | MS.gene35109:CDS |
| GAGGTTGCTGTGGTTCAATC+TGG | 0.485796 | 4.4:-19868633 | MS.gene35109:CDS |
| TGGGAGAAGAGTAAAGTTCA+TGG | 0.492553 | 4.4:-19868579 | MS.gene35109:CDS |
| GTTCAATCTGGTGCTATACT+TGG | 0.497152 | 4.4:-19868621 | MS.gene35109:CDS |
| TGAGTTCTAAGGTTGTAAAA+GGG | 0.501382 | 4.4:-19868173 | MS.gene35109:CDS |
| GATTCTTGATAGGAAATCAA+TGG | 0.502476 | 4.4:-19868421 | MS.gene35109:CDS |
| GAAGAGGAAATCTGATTATG+TGG | 0.503029 | 4.4:-19867938 | MS.gene35109:CDS |
| AAAAGCCTATCATCGGTATG+TGG | 0.507246 | 4.4:+19868219 | None:intergenic |
| TATTACTTACTAGGGTGAGT+TGG | 0.509730 | 4.4:+19867472 | None:intergenic |
| AACCAGATGAGCTTCTTGAC+AGG | 0.512231 | 4.4:-19867984 | MS.gene35109:CDS |
| GCTGGGGTTTGTCCTACTGT+TGG | 0.514036 | 4.4:-19868549 | MS.gene35109:CDS |
| TGTTCTGAAATGAGTTGGAT+TGG | 0.514378 | 4.4:-19868042 | MS.gene35109:CDS |
| GGATTGGTTCTGTGCTTTGG+TGG | 0.514397 | 4.4:-19868026 | MS.gene35109:CDS |
| AATCATTAGTACCAATATCA+AGG | 0.514587 | 4.4:+19867623 | None:intergenic |
| ATTCGGCAAAATTTCTGAAG+AGG | 0.515257 | 4.4:-19867954 | MS.gene35109:CDS |
| AGTTCATGGCTTTCCTGCTG+GGG | 0.515278 | 4.4:-19868565 | MS.gene35109:CDS |
| CCGCGTTTCCTCACCGTGCA+GGG | 0.515673 | 4.4:-19867795 | MS.gene35109:CDS |
| GAGAATACAATATCAAAGGA+TGG | 0.517021 | 4.4:-19867916 | MS.gene35109:CDS |
| CACTGTTGATATACAAAATG+AGG | 0.521469 | 4.4:-19868652 | MS.gene35109:CDS |
| GGAAATAATAGTTATGAACA+AGG | 0.533347 | 4.4:-19867598 | MS.gene35109:CDS |
| GTTAGAAGGGATATGGAAGA+AGG | 0.533370 | 4.4:-19867893 | MS.gene35109:CDS |
| ACAGTAGGACAAACCCCAGC+AGG | 0.538276 | 4.4:+19868552 | None:intergenic |
| TGGGCTATTAGAGGAGGTGG+AGG | 0.539964 | 4.4:-19868384 | MS.gene35109:CDS |
| CCATCCTCTATTACTTACTA+GGG | 0.551020 | 4.4:+19867464 | None:intergenic |
| TCTTTCTTCAAACCAAGAGA+AGG | 0.556484 | 4.4:+19868069 | None:intergenic |
| TATCAAAGGATGGGTTAGAA+GGG | 0.560336 | 4.4:-19867906 | MS.gene35109:CDS |
| TTTACAACCTTAGAACTCAC+AGG | 0.562752 | 4.4:+19868177 | None:intergenic |
| GTGGTCACTTGAGTGGTGGA+GGG | 0.563379 | 4.4:-19868521 | MS.gene35109:CDS |
| GTTGGTGGTCACTTGAGTGG+TGG | 0.568531 | 4.4:-19868525 | MS.gene35109:CDS |
| AAAGCCTATCATCGGTATGT+GGG | 0.569559 | 4.4:+19868220 | None:intergenic |
| TGTGGAGAATACAATATCAA+AGG | 0.573640 | 4.4:-19867920 | MS.gene35109:CDS |
| ATGAACTTGTTACACTATTG+GGG | 0.574341 | 4.4:-19868101 | MS.gene35109:CDS |
| ACTTGTTACACTATTGGGGA+AGG | 0.576337 | 4.4:-19868097 | MS.gene35109:CDS |
| CCCTGCACGGTGAGGAAACG+CGG | 0.583533 | 4.4:+19867795 | None:intergenic |
| GGTGTTGGTGGTCACTTGAG+TGG | 0.589213 | 4.4:-19868528 | MS.gene35109:CDS |
| AGAATACAATATCAAAGGAT+GGG | 0.591063 | 4.4:-19867915 | MS.gene35109:CDS |
| TCGGTGAATTGGGATAAGCC+TGG | 0.595224 | 4.4:-19867751 | MS.gene35109:CDS |
| AAATTGTTGATGTTAATGGT+AGG | 0.611718 | 4.4:-19868443 | MS.gene35109:CDS |
| CCTCATGAAAAGCCTATCAT+CGG | 0.611966 | 4.4:+19868212 | None:intergenic |
| AAGCCTATCATCGGTATGTG+GGG | 0.620231 | 4.4:+19868221 | None:intergenic |
| GAGTTCTAAGGTTGTAAAAG+GGG | 0.622042 | 4.4:-19868172 | MS.gene35109:CDS |
| TCTTGAATAGATTCCCTGCA+CGG | 0.644782 | 4.4:+19867782 | None:intergenic |
| TGACCACCAACACCAACAGT+AGG | 0.644936 | 4.4:+19868537 | None:intergenic |
| AATAGATTCCCTGCACGGTG+AGG | 0.655048 | 4.4:+19867787 | None:intergenic |
| TCACTGAGTTTGTGTTGCAG+TGG | 0.672314 | 4.4:-19868254 | MS.gene35109:CDS |
| TATACAAAATGAGGTTGCTG+TGG | 0.675121 | 4.4:-19868643 | MS.gene35109:CDS |
| TCTTGATAGGAAATCAATGG+GGG | 0.683281 | 4.4:-19868418 | MS.gene35109:CDS |
| TTCTTGATAGGAAATCAATG+GGG | 0.706685 | 4.4:-19868419 | MS.gene35109:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAAAATAATTTTACAAATC+AGG | - | chr4.4:19868427-19868446 | MS.gene35109:CDS | 15.0% |
| !! | TGAAGTTTATTATAGGATAT+GGG | - | chr4.4:19867548-19867567 | MS.gene35109:CDS | 20.0% |
| !! | TTTATCATACAGTATTAAGT+TGG | - | chr4.4:19867803-19867822 | MS.gene35109:CDS | 20.0% |
| !!! | AAGAGCCTTTTTAAATTATA+GGG | - | chr4.4:19868499-19868518 | MS.gene35109:CDS | 20.0% |
| !!! | TTATTTTCTATATCAATTCC+AGG | + | chr4.4:19868416-19868435 | None:intergenic | 20.0% |
| ! | AAATTGTTGATGTTAATGGT+AGG | - | chr4.4:19867703-19867722 | MS.gene35109:CDS | 25.0% |
| ! | AAGGTCCCTATAATTTAAAA+AGG | + | chr4.4:19868507-19868526 | None:intergenic | 25.0% |
| ! | AATCATTAGTACCAATATCA+AGG | + | chr4.4:19868526-19868545 | None:intergenic | 25.0% |
| ! | AGAATACAATATCAAAGGAT+GGG | - | chr4.4:19868231-19868250 | MS.gene35109:CDS | 25.0% |
| ! | ATTCTTGATAGGAAATCAAT+GGG | - | chr4.4:19867726-19867745 | MS.gene35109:CDS | 25.0% |
| ! | GGAAATAATAGTTATGAACA+AGG | - | chr4.4:19868548-19868567 | MS.gene35109:CDS | 25.0% |
| ! | GGTAATATGATGAGAAAATT+TGG | - | chr4.4:19867651-19867670 | MS.gene35109:CDS | 25.0% |
| ! | GTGAAGTTTATTATAGGATA+TGG | - | chr4.4:19867547-19867566 | MS.gene35109:CDS | 25.0% |
| ! | TAATTTAAAAAGGCTCTTCT+TGG | + | chr4.4:19868497-19868516 | None:intergenic | 25.0% |
| ! | TCTATTCAAGATTCAGTATT+CGG | - | chr4.4:19868376-19868395 | MS.gene35109:CDS | 25.0% |
| !! | ATTTCAGTAACAATTTTGAG+AGG | - | chr4.4:19868591-19868610 | MS.gene35109:CDS | 25.0% |
| !! | GAAGAGCCTTTTTAAATTAT+AGG | - | chr4.4:19868498-19868517 | MS.gene35109:CDS | 25.0% |
| !! | GATATTGGTACTAATGATTT+TGG | - | chr4.4:19868527-19868546 | MS.gene35109:CDS | 25.0% |
| !! | TACTTGGTGAAGTTTATTAT+AGG | - | chr4.4:19867541-19867560 | MS.gene35109:CDS | 25.0% |
| !!! | AAAATTGTTCTGAAATGAGT+TGG | - | chr4.4:19868099-19868118 | MS.gene35109:CDS | 25.0% |
| AATTATAGGGACCTTGATAT+TGG | - | chr4.4:19868512-19868531 | MS.gene35109:CDS | 30.0% | |
| CACTGTTGATATACAAAATG+AGG | - | chr4.4:19867494-19867513 | MS.gene35109:CDS | 30.0% | |
| CGAAAAACAGTAACAATTTC+AGG | + | chr4.4:19867837-19867856 | None:intergenic | 30.0% | |
| CTTGGATTATTGGAAACAAA+AGG | + | chr4.4:19868479-19868498 | None:intergenic | 30.0% | |
| GAGAATACAATATCAAAGGA+TGG | - | chr4.4:19868230-19868249 | MS.gene35109:CDS | 30.0% | |
| GATTCTTGATAGGAAATCAA+TGG | - | chr4.4:19867725-19867744 | MS.gene35109:CDS | 30.0% | |
| GCTGAAATTGTTGATGTTAA+TGG | - | chr4.4:19867699-19867718 | MS.gene35109:CDS | 30.0% | |
| TATGAACAAGGAGTTGTTTA+TGG | - | chr4.4:19868560-19868579 | MS.gene35109:CDS | 30.0% | |
| TGTGGAGAATACAATATCAA+AGG | - | chr4.4:19868226-19868245 | MS.gene35109:CDS | 30.0% | |
| TTAATGGTAGGATTCTTGAT+AGG | - | chr4.4:19867715-19867734 | MS.gene35109:CDS | 30.0% | |
| TTCTTGATAGGAAATCAATG+GGG | - | chr4.4:19867727-19867746 | MS.gene35109:CDS | 30.0% | |
| TTGATCCAGAAAATTTCTTC+AGG | - | chr4.4:19868636-19868655 | MS.gene35109:CDS | 30.0% | |
| ! | TCATTCCTGAAGAAATTTTC+TGG | + | chr4.4:19868644-19868663 | None:intergenic | 30.0% |
| ! | TCTTGACAGGAATATTGATT+CGG | - | chr4.4:19868175-19868194 | MS.gene35109:CDS | 30.0% |
| !! | ATGAACTTGTTACACTATTG+GGG | - | chr4.4:19868045-19868064 | MS.gene35109:CDS | 30.0% |
| !! | GATGAACTTGTTACACTATT+GGG | - | chr4.4:19868044-19868063 | MS.gene35109:CDS | 30.0% |
| !! | TGAGTTCTAAGGTTGTAAAA+GGG | - | chr4.4:19867973-19867992 | MS.gene35109:CDS | 30.0% |
| !! | TGATGAACTTGTTACACTAT+TGG | - | chr4.4:19868043-19868062 | MS.gene35109:CDS | 30.0% |
| AAAGGCTCTTCTTGGATTAT+TGG | + | chr4.4:19868489-19868508 | None:intergenic | 35.0% | |
| AACTGTTAGAGTGACTGTTA+TGG | - | chr4.4:19868001-19868020 | MS.gene35109:CDS | 35.0% | |
| ATGGAAGAAGGTGATTGAAT+TGG | - | chr4.4:19868265-19868284 | MS.gene35109:CDS | 35.0% | |
| GAAGAGGAAATCTGATTATG+TGG | - | chr4.4:19868208-19868227 | MS.gene35109:CDS | 35.0% | |
| GTGATTGAATTGGGAAAAGT+AGG | - | chr4.4:19868275-19868294 | MS.gene35109:CDS | 35.0% | |
| TATACAAAATGAGGTTGCTG+TGG | - | chr4.4:19867503-19867522 | MS.gene35109:CDS | 35.0% | |
| TCTTGATAGGAAATCAATGG+GGG | - | chr4.4:19867728-19867747 | MS.gene35109:CDS | 35.0% | |
| TCTTTCTTCAAACCAAGAGA+AGG | + | chr4.4:19868080-19868099 | None:intergenic | 35.0% | |
| TGAATTGGGAAAAGTAGGAT+TGG | - | chr4.4:19868280-19868299 | MS.gene35109:CDS | 35.0% | |
| TGGAAGAAGGTGATTGAATT+GGG | - | chr4.4:19868266-19868285 | MS.gene35109:CDS | 35.0% | |
| TTTACAACCTTAGAACTCAC+AGG | + | chr4.4:19867972-19867991 | None:intergenic | 35.0% | |
| ! | AGATTCAGTATTCGGTGAAT+TGG | - | chr4.4:19868384-19868403 | MS.gene35109:CDS | 35.0% |
| ! | ATTCGGCAAAATTTCTGAAG+AGG | - | chr4.4:19868192-19868211 | MS.gene35109:CDS | 35.0% |
| ! | GATTCAGTATTCGGTGAATT+GGG | - | chr4.4:19868385-19868404 | MS.gene35109:CDS | 35.0% |
| !! | ACTGTTATGGCTTTGTTTCT+TGG | - | chr4.4:19868014-19868033 | MS.gene35109:CDS | 35.0% |
| !! | ATATCAAAGGATGGGTTAGA+AGG | - | chr4.4:19868239-19868258 | MS.gene35109:CDS | 35.0% |
| !! | CAGTAACAATTTTGAGAGGT+TGG | - | chr4.4:19868595-19868614 | MS.gene35109:CDS | 35.0% |
| !! | GAGTTCTAAGGTTGTAAAAG+GGG | - | chr4.4:19867974-19867993 | MS.gene35109:CDS | 35.0% |
| !! | GTGAGTTCTAAGGTTGTAAA+AGG | - | chr4.4:19867972-19867991 | MS.gene35109:CDS | 35.0% |
| !! | TATCAAAGGATGGGTTAGAA+GGG | - | chr4.4:19868240-19868259 | MS.gene35109:CDS | 35.0% |
| !! | TGTTACTGTTTTTCGTGTTG+CGG | - | chr4.4:19867842-19867861 | MS.gene35109:CDS | 35.0% |
| !! | TGTTCTGAAATGAGTTGGAT+TGG | - | chr4.4:19868104-19868123 | MS.gene35109:CDS | 35.0% |
| !!! | AATGGGGGAAGAACTTTTTT+GGG | - | chr4.4:19867743-19867762 | MS.gene35109:CDS | 35.0% |
| !!! | GAACTTTTTTGGGCTATTAG+AGG | - | chr4.4:19867753-19867772 | MS.gene35109:CDS | 35.0% |
| !!! | GGATTGGTTTTCAATCCTTA+CGG | - | chr4.4:19868296-19868315 | MS.gene35109:CDS | 35.0% |
| AAAAGCCTATCATCGGTATG+TGG | + | chr4.4:19867930-19867949 | None:intergenic | 40.0% | |
| AAAGCCTATCATCGGTATGT+GGG | + | chr4.4:19867929-19867948 | None:intergenic | 40.0% | |
| CCTCATGAAAAGCCTATCAT+CGG | + | chr4.4:19867937-19867956 | None:intergenic | 40.0% | |
| GTTAGAAGGGATATGGAAGA+AGG | - | chr4.4:19868253-19868272 | MS.gene35109:CDS | 40.0% | |
| TATTCAGCCTGTGAGTTCTA+AGG | - | chr4.4:19867962-19867981 | MS.gene35109:CDS | 40.0% | |
| TCTTGAATAGATTCCCTGCA+CGG | + | chr4.4:19868367-19868386 | None:intergenic | 40.0% | |
| TGGGAGAAGAGTAAAGTTCA+TGG | - | chr4.4:19867567-19867586 | MS.gene35109:CDS | 40.0% | |
| ! | ACTTGTTACACTATTGGGGA+AGG | - | chr4.4:19868049-19868068 | MS.gene35109:CDS | 40.0% |
| !! | GAGTTCATTTTTCCACCGTA+AGG | + | chr4.4:19868314-19868333 | None:intergenic | 40.0% |
| !! | GTTATGGCTTTGTTTCTTGG+AGG | - | chr4.4:19868017-19868036 | MS.gene35109:CDS | 40.0% |
| !! | GTTCAATCTGGTGCTATACT+TGG | - | chr4.4:19867525-19867544 | MS.gene35109:CDS | 40.0% |
| !! | TTGGTTTTCAATCCTTACGG+TGG | - | chr4.4:19868299-19868318 | MS.gene35109:CDS | 40.0% |
| !!! | CAATGGGGGAAGAACTTTTT+TGG | - | chr4.4:19867742-19867761 | MS.gene35109:CDS | 40.0% |
| !!! | CTTTTTTGGGCTATTAGAGG+AGG | - | chr4.4:19867756-19867775 | MS.gene35109:CDS | 40.0% |
| AAAGTTCATGGCTTTCCTGC+TGG | - | chr4.4:19867579-19867598 | MS.gene35109:CDS | 45.0% | |
| AACCAGATGAGCTTCTTGAC+AGG | - | chr4.4:19868162-19868181 | MS.gene35109:CDS | 45.0% | |
| AAGCCTATCATCGGTATGTG+GGG | + | chr4.4:19867928-19867947 | None:intergenic | 45.0% | |
| AAGTTCATGGCTTTCCTGCT+GGG | - | chr4.4:19867580-19867599 | MS.gene35109:CDS | 45.0% | |
| GTTTGTCCTACTGTTGGTGT+TGG | - | chr4.4:19867603-19867622 | MS.gene35109:CDS | 45.0% | |
| TCACTGAGTTTGTGTTGCAG+TGG | - | chr4.4:19867892-19867911 | MS.gene35109:CDS | 45.0% | |
| TGCGGAGAGTTTGGATAGTT+TGG | - | chr4.4:19867860-19867879 | MS.gene35109:CDS | 45.0% | |
| TTCCTGTCAAGAAGCTCATC+TGG | + | chr4.4:19868167-19868186 | None:intergenic | 45.0% | |
| ! | CCGATGATAGGCTTTTCATG+AGG | - | chr4.4:19867934-19867953 | MS.gene35109:CDS | 45.0% |
| ! | GGGAAGGATTTTCCTTCTCT+TGG | - | chr4.4:19868065-19868084 | MS.gene35109:CDS | 45.0% |
| ! | TTTTCGTGTTGCGGAGAGTT+TGG | - | chr4.4:19867851-19867870 | MS.gene35109:CDS | 45.0% |
| ! | TTTTGGGCTATTAGAGGAGG+TGG | - | chr4.4:19867759-19867778 | MS.gene35109:CDS | 45.0% |
| !! | AGGATGGGTTAGAAGGGATA+TGG | - | chr4.4:19868246-19868265 | MS.gene35109:CDS | 45.0% |
| !! | GATTGGTTCTGTGCTTTGGT+GGG | - | chr4.4:19868121-19868140 | MS.gene35109:CDS | 45.0% |
| !! | GTTGGATTGGTTCTGTGCTT+TGG | - | chr4.4:19868117-19868136 | MS.gene35109:CDS | 45.0% |
| AATAGATTCCCTGCACGGTG+AGG | + | chr4.4:19868362-19868381 | None:intergenic | 50.0% | |
| AGTTCATGGCTTTCCTGCTG+GGG | - | chr4.4:19867581-19867600 | MS.gene35109:CDS | 50.0% | |
| GAGGTTGCTGTGGTTCAATC+TGG | - | chr4.4:19867513-19867532 | MS.gene35109:CDS | 50.0% | |
| TCGGTGAATTGGGATAAGCC+TGG | - | chr4.4:19868395-19868414 | MS.gene35109:CDS | 50.0% | |
| TGACCACCAACACCAACAGT+AGG | + | chr4.4:19867612-19867631 | None:intergenic | 50.0% | |
| TTGCCCCACATACCGATGAT+AGG | - | chr4.4:19867922-19867941 | MS.gene35109:CDS | 50.0% | |
| !! | GGATTGGTTCTGTGCTTTGG+TGG | - | chr4.4:19868120-19868139 | MS.gene35109:CDS | 50.0% |
| !! | GTGCTTTGGTGGGCTAGTAT+TGG | - | chr4.4:19868131-19868150 | MS.gene35109:CDS | 50.0% |
| !! | TGTCCTACTGTTGGTGTTGG+TGG | - | chr4.4:19867606-19867625 | MS.gene35109:CDS | 50.0% |
| ACAGTAGGACAAACCCCAGC+AGG | + | chr4.4:19867597-19867616 | None:intergenic | 55.0% | |
| GCTGGGGTTTGTCCTACTGT+TGG | - | chr4.4:19867597-19867616 | MS.gene35109:CDS | 55.0% | |
| TGGGCTATTAGAGGAGGTGG+AGG | - | chr4.4:19867762-19867781 | MS.gene35109:CDS | 55.0% | |
| ! | CACTTGAGTGGTGGAGGGTA+TGG | - | chr4.4:19867630-19867649 | MS.gene35109:CDS | 55.0% |
| ! | GTGGTCACTTGAGTGGTGGA+GGG | - | chr4.4:19867625-19867644 | MS.gene35109:CDS | 55.0% |
| !! | GGTGTTGGTGGTCACTTGAG+TGG | - | chr4.4:19867618-19867637 | MS.gene35109:CDS | 55.0% |
| !! | GTTGGTGGTCACTTGAGTGG+TGG | - | chr4.4:19867621-19867640 | MS.gene35109:CDS | 55.0% |
| GGTGGTCACTTGAGTGGTGG+AGG | - | chr4.4:19867624-19867643 | MS.gene35109:CDS | 60.0% | |
| ! | GGAGGTGGAGGAGCGAGTTT+TGG | - | chr4.4:19867774-19867793 | MS.gene35109:CDS | 60.0% |
| ACCGCGTTTCCTCACCGTGC+AGG | - | chr4.4:19868350-19868369 | MS.gene35109:CDS | 65.0% | |
| CCCTGCACGGTGAGGAAACG+CGG | + | chr4.4:19868354-19868373 | None:intergenic | 65.0% | |
| CCGCGTTTCCTCACCGTGCA+GGG | - | chr4.4:19868351-19868370 | MS.gene35109:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 19867474 | 19868694 | 19867474 | ID=MS.gene35109 |
| chr4.4 | mRNA | 19867474 | 19868694 | 19867474 | ID=MS.gene35109.t1;Parent=MS.gene35109 |
| chr4.4 | exon | 19867474 | 19868694 | 19867474 | ID=MS.gene35109.t1.exon1;Parent=MS.gene35109.t1 |
| chr4.4 | CDS | 19867474 | 19868694 | 19867474 | ID=cds.MS.gene35109.t1;Parent=MS.gene35109.t1 |
| Gene Sequence |
| Protein sequence |
