Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35484.t1 | XP_003602090.2 | 98.2 | 56 | 1 | 0 | 1 | 56 | 58 | 113 | 8.30E-25 | 122.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35484.t1 | Q84W56 | 89.3 | 56 | 6 | 0 | 1 | 56 | 90 | 145 | 4.1e-25 | 114.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35484.t1 | G7J5I7 | 98.2 | 56 | 1 | 0 | 1 | 56 | 58 | 113 | 6.0e-25 | 122.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055539 | MS.gene35484 | 0.823841 | 1.11E-53 | -1.69E-46 |
| MS.gene061231 | MS.gene35484 | 0.838652 | 2.53E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35484.t1 | MTR_3g089020 | 98.214 | 56 | 1 | 0 | 1 | 56 | 58 | 113 | 2.73e-36 | 127 |
| MS.gene35484.t1 | MTR_4g103770 | 92.727 | 55 | 4 | 0 | 1 | 55 | 62 | 116 | 1.75e-34 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35484.t1 | AT5G63420 | 89.286 | 56 | 6 | 0 | 1 | 56 | 90 | 145 | 3.29e-33 | 119 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTGGTTTGGGTGAAAT+TGG | 0.278425 | 3.4:-73464775 | MS.gene35484:CDS |
| CCATTACGTGTTCTTCCTAT+TGG | 0.296621 | 3.4:-73464793 | MS.gene35484:CDS |
| TGTTCTTCCTATTGGTGGTT+TGG | 0.367711 | 3.4:-73464785 | MS.gene35484:CDS |
| GTTCTTCCTATTGGTGGTTT+GGG | 0.382494 | 3.4:-73464784 | MS.gene35484:CDS |
| GAATGAATTGTATGCTTGTT+GGG | 0.394201 | 3.4:-73464753 | MS.gene35484:CDS |
| GGAATGAATTGTATGCTTGT+TGG | 0.453098 | 3.4:-73464754 | MS.gene35484:CDS |
| TTACGTGTTCTTCCTATTGG+TGG | 0.453954 | 3.4:-73464790 | MS.gene35484:CDS |
| ATTTCACCCAAACCACCAAT+AGG | 0.461698 | 3.4:+73464778 | None:intergenic |
| ACTATTAAGGACCACTTACT+CGG | 0.483080 | 3.4:+73464687 | None:intergenic |
| CGTTATATCCTCATTGATGC+TGG | 0.516389 | 3.4:-73464721 | MS.gene35484:CDS |
| CAGCATCAATGAGGATATAA+CGG | 0.531872 | 3.4:+73464722 | None:intergenic |
| AGATTCTGTTCAACGTAGGA+TGG | 0.542555 | 3.4:-73464845 | MS.gene35484:CDS |
| CTATTAAGGACCACTTACTC+GGG | 0.570977 | 3.4:+73464688 | None:intergenic |
| CCAATAGGAAGAACACGTAA+TGG | 0.600542 | 3.4:+73464793 | None:intergenic |
| ACATGATACCAGCATCAATG+AGG | 0.660800 | 3.4:+73464713 | None:intergenic |
| TGGAAGATTCTGTTCAACGT+AGG | 0.664297 | 3.4:-73464849 | MS.gene35484:CDS |
| ATAGGAAGAACACGTAATGG+CGG | 0.726743 | 3.4:+73464796 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAATGAATTGTATGCTTGTT+GGG | - | chr3.4:73464799-73464818 | MS.gene35484:CDS | 30.0% | |
| ! | CAATTTTATGAAGGAAACGA+CGG | - | chr3.4:73464732-73464751 | MS.gene35484:CDS | 30.0% |
| CAGCATCAATGAGGATATAA+CGG | + | chr3.4:73464833-73464852 | None:intergenic | 35.0% | |
| GGAATGAATTGTATGCTTGT+TGG | - | chr3.4:73464798-73464817 | MS.gene35484:CDS | 35.0% | |
| ! | AGGATGGAGCAATTTTATGA+AGG | - | chr3.4:73464723-73464742 | MS.gene35484:CDS | 35.0% |
| ACATGATACCAGCATCAATG+AGG | + | chr3.4:73464842-73464861 | None:intergenic | 40.0% | |
| AGATTCTGTTCAACGTAGGA+TGG | - | chr3.4:73464707-73464726 | MS.gene35484:CDS | 40.0% | |
| ATAGGAAGAACACGTAATGG+CGG | + | chr3.4:73464759-73464778 | None:intergenic | 40.0% | |
| ATTTCACCCAAACCACCAAT+AGG | + | chr3.4:73464777-73464796 | None:intergenic | 40.0% | |
| CCAATAGGAAGAACACGTAA+TGG | + | chr3.4:73464762-73464781 | None:intergenic | 40.0% | |
| CCATTACGTGTTCTTCCTAT+TGG | - | chr3.4:73464759-73464778 | MS.gene35484:CDS | 40.0% | |
| CGTTATATCCTCATTGATGC+TGG | - | chr3.4:73464831-73464850 | MS.gene35484:CDS | 40.0% | |
| TGGAAGATTCTGTTCAACGT+AGG | - | chr3.4:73464703-73464722 | MS.gene35484:CDS | 40.0% | |
| TTACGTGTTCTTCCTATTGG+TGG | - | chr3.4:73464762-73464781 | MS.gene35484:CDS | 40.0% | |
| ! | ATTGGTGGTTTGGGTGAAAT+TGG | - | chr3.4:73464777-73464796 | MS.gene35484:CDS | 40.0% |
| ! | GTTCTTCCTATTGGTGGTTT+GGG | - | chr3.4:73464768-73464787 | MS.gene35484:CDS | 40.0% |
| ! | TGTTCTTCCTATTGGTGGTT+TGG | - | chr3.4:73464767-73464786 | MS.gene35484:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 73464702 | 73464872 | 73464702 | ID=MS.gene35484 |
| chr3.4 | mRNA | 73464702 | 73464872 | 73464702 | ID=MS.gene35484.t1;Parent=MS.gene35484 |
| chr3.4 | exon | 73464702 | 73464872 | 73464702 | ID=MS.gene35484.t1.exon1;Parent=MS.gene35484.t1 |
| chr3.4 | CDS | 73464702 | 73464872 | 73464702 | ID=cds.MS.gene35484.t1;Parent=MS.gene35484.t1 |
| Gene Sequence |
| Protein sequence |
