AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35578


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35578.t1 MTR_3g074830 97.476 317 7 1 1 316 29 345 0.0 631
MS.gene35578.t1 MTR_5g064360 70.846 319 87 4 1 316 1 316 1.76e-162 455
MS.gene35578.t1 MTR_5g464340 71.787 319 83 5 1 316 1 315 6.61e-162 454
MS.gene35578.t1 MTR_5g064340 71.787 319 83 5 1 316 1 315 6.61e-162 454
MS.gene35578.t1 MTR_5g064345 72.436 312 78 5 8 316 9 315 8.56e-161 451
MS.gene35578.t1 MTR_3g074860 70.470 298 74 4 19 316 21 304 5.03e-150 423
MS.gene35578.t1 MTR_3g074890 69.799 298 76 4 19 316 21 304 2.08e-148 419
MS.gene35578.t1 MTR_4g094240 61.512 291 99 3 26 316 23 300 1.92e-127 366
MS.gene35578.t1 MTR_2g009070 54.397 307 122 6 11 316 15 304 7.32e-120 347
MS.gene35578.t1 MTR_1g076470 37.818 275 151 9 38 301 64 329 4.10e-43 151
MS.gene35578.t1 MTR_4g033790 37.993 279 152 9 38 305 65 333 2.10e-42 150
MS.gene35578.t1 MTR_8g071310 88.235 68 8 0 249 316 18 85 1.21e-35 124
MS.gene35578.t1 MTR_8g088900 74.444 90 23 0 227 316 283 372 9.76e-35 130
MS.gene35578.t1 MTR_4g013230 92.308 65 2 1 252 316 79 140 2.68e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35578.t1 AT4G08950 73.090 301 74 4 20 316 17 314 6.78e-157 441
MS.gene35578.t1 AT1G35140 63.226 310 106 4 8 315 5 308 9.31e-139 395
MS.gene35578.t1 AT5G64260 57.367 319 119 5 1 316 1 305 4.37e-127 365
MS.gene35578.t1 AT5G09440 52.516 318 109 8 1 316 1 278 2.30e-101 299
MS.gene35578.t1 AT3G02970 34.228 298 161 6 48 314 37 330 2.18e-49 167
MS.gene35578.t1 AT5G51550 39.024 287 154 9 38 313 55 331 8.45e-47 161
MS.gene35578.t1 AT2G35150 36.923 325 176 14 7 312 2 316 3.24e-41 146
MS.gene35578.t1 AT2G17230 35.473 296 168 10 32 313 71 357 2.38e-40 145

Find 67 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTTGTCCTGGTGTTTA+TGG 0.171889 3.3:+64390754 MS.gene35578:CDS
ATCAAGAATTTGCTTGTTTA+GGG 0.208436 3.3:-64390308 None:intergenic
CATCAAGAATTTGCTTGTTT+AGG 0.214856 3.3:-64390309 None:intergenic
GTTCACCTTTGGAAGCTAAT+TGG 0.254782 3.3:-64390375 None:intergenic
TTTGAGAAGGGATTGAAAAT+TGG 0.273364 3.3:-64389981 None:intergenic
TTGCAAAATTTGCTTACATT+TGG 0.273951 3.3:+64390515 MS.gene35578:CDS
TGATGGAATGGTGATAAATT+TGG 0.283166 3.3:+64390648 MS.gene35578:CDS
CTTGATGAGAATTACTCATT+AGG 0.306480 3.3:+64390325 MS.gene35578:CDS
GCGCTTTGTGGACCGTAAAT+TGG 0.317106 3.3:-64390592 None:intergenic
CCATTTCACCAACCAATTTA+CGG 0.318307 3.3:+64390580 MS.gene35578:CDS
TGCAAAATTTGCTTACATTT+GGG 0.320080 3.3:+64390516 MS.gene35578:CDS
ACACGGTTCTTCTTCTTCAA+TGG 0.340114 3.3:+64390474 MS.gene35578:CDS
TTTGGAAATGGTTACTTTCA+AGG 0.342445 3.3:+64390703 MS.gene35578:CDS
TTCAATGCTCATGGTGATAA+TGG 0.347620 3.3:+64390838 MS.gene35578:CDS
TTGCTTGTTGATTCTACTAC+TGG 0.362064 3.3:+64390808 MS.gene35578:CDS
GGAACTGCAACAAACCCTTT+TGG 0.395578 3.3:+64390685 MS.gene35578:CDS
CCGTAAATTGGTTGGTGAAA+TGG 0.399220 3.3:-64390580 None:intergenic
GGAATCTTTGTGTTCACCTT+TGG 0.401182 3.3:-64390386 None:intergenic
CACAACGGTCCATTACTCTA+TGG 0.410127 3.3:+64390094 MS.gene35578:CDS
AACAACGATGTAGGGCTTGA+TGG 0.410771 3.3:+64390631 MS.gene35578:CDS
AAATCTCAGTCAATTTAATC+TGG 0.417323 3.3:+64390119 MS.gene35578:CDS
GCAACCAATGGTGCGCTTTG+TGG 0.422076 3.3:-64390604 None:intergenic
TCAAGCCCTACATCGTTGTT+TGG 0.423713 3.3:-64390628 None:intergenic
TGAAAGTAACCATTTCCAAA+AGG 0.429612 3.3:-64390700 None:intergenic
GAAAGTAACCATTTCCAAAA+GGG 0.440576 3.3:-64390699 None:intergenic
GTTGAGCAAGATAATGTAGA+AGG 0.441408 3.3:-64390892 None:intergenic
GCTGAAACTGAAGGTTGGTT+TGG 0.443189 3.3:-64390217 None:intergenic
GAAGCTGCTTCAGCTTGTCC+TGG 0.453010 3.3:+64390745 MS.gene35578:CDS
AGATGTTAGTACAACGTTGA+TGG 0.462886 3.3:-64390407 None:intergenic
AGTTTCTAGCAGCTGAAGAA+AGG 0.492873 3.3:-64390030 None:intergenic
TGTCCTGGTGTTTATGGTAA+AGG 0.498489 3.3:+64390760 MS.gene35578:CDS
ACCATGCTGAAACTGAAGGT+TGG 0.500598 3.3:-64390222 None:intergenic
TCAGTCAATTTAATCTGGTA+TGG 0.500777 3.3:+64390124 MS.gene35578:CDS
GGTGCTTACCCTGGTTATGC+TGG 0.504722 3.3:+64390781 MS.gene35578:CDS
AAATTTGCTTACATTTGGGT+TGG 0.505466 3.3:+64390520 MS.gene35578:CDS
AACTCAGAAACACAGTGTCC+AGG 0.509516 3.3:+64390544 MS.gene35578:CDS
TCTTCAATGGAATCCAGAAA+AGG 0.511852 3.3:+64390487 MS.gene35578:CDS
CGGTCCACAAAGCGCACCAT+TGG 0.512236 3.3:+64390600 MS.gene35578:CDS
AGCAAATCACCAGCATAACC+AGG 0.517128 3.3:-64390790 None:intergenic
AGTGTCCAGGGTTATGTGCT+TGG 0.528873 3.3:+64390557 MS.gene35578:CDS
TTGCACCAAACAACGATGTA+GGG 0.528944 3.3:+64390623 MS.gene35578:CDS
CGATGTAGGGCTTGATGGAA+TGG 0.529717 3.3:+64390636 MS.gene35578:CDS
GTTGCACCAAACAACGATGT+AGG 0.531920 3.3:+64390622 MS.gene35578:CDS
AATGGAATCCAGAAAAGGTA+AGG 0.547288 3.3:+64390492 MS.gene35578:CDS
ATGCTCATGGTGATAATGGA+AGG 0.567528 3.3:+64390842 MS.gene35578:CDS
ACTCAGAAACACAGTGTCCA+GGG 0.569464 3.3:+64390545 MS.gene35578:CDS
ATTATCCAATTAGCTTCCAA+AGG 0.570977 3.3:+64390370 MS.gene35578:CDS
TTCCACCATGCTGAAACTGA+AGG 0.570995 3.3:-64390226 None:intergenic
TTTGTGGACCGTAAATTGGT+TGG 0.572936 3.3:-64390588 None:intergenic
GTAGAAGGATCATACAAAGC+AGG 0.575060 3.3:-64390877 None:intergenic
TATGGTAAAGGTGCTTACCC+TGG 0.580611 3.3:+64390772 MS.gene35578:CDS
ATTGCAATGTTCTTCAAACA+AGG 0.583966 3.3:-64390915 None:intergenic
GGTGCTAGTTTCAATGCTCA+TGG 0.589796 3.3:+64390829 MS.gene35578:CDS
AACCTTCAGTTTCAGCATGG+TGG 0.591696 3.3:+64390224 MS.gene35578:CDS
TTGCAATGTTCTTCAAACAA+GGG 0.601668 3.3:-64390914 None:intergenic
GCTTCAAGAGGAGCTTCAGA+AGG 0.602180 3.3:-64390727 None:intergenic
TCGTTGTTTGGTGCAACCAA+TGG 0.602504 3.3:-64390616 None:intergenic
TGAAGATGGTGAAGAAAGTG+AGG 0.607624 3.3:-64390191 None:intergenic
ATGGACCGATGTGGCACACA+CGG 0.610497 3.3:+64390457 MS.gene35578:CDS
AAGAACCGTGTGTGCCACAT+CGG 0.625233 3.3:-64390462 None:intergenic
CAAGCTGAAGCAGCTTCAAG+AGG 0.626827 3.3:-64390739 None:intergenic
ACCAACCTTCAGTTTCAGCA+TGG 0.631067 3.3:+64390221 MS.gene35578:CDS
AATGGCCAAGCACATAACCC+TGG 0.674129 3.3:-64390562 None:intergenic
CATAGAGTAATGGACCGTTG+TGG 0.689245 3.3:-64390093 None:intergenic
CAGCTTCTTCATTACCACAA+CGG 0.692004 3.3:+64390079 MS.gene35578:CDS
GCAAATCACCAGCATAACCA+GGG 0.695085 3.3:-64390789 None:intergenic
GCACCTTTACCATAAACACC+AGG 0.705559 3.3:-64390763 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAGAAAAAATTTGAGAA+GGG - chr3.3:64389996-64390015 None:intergenic 20.0%
!! TAAGAAGAAAAAATTTGAGA+AGG - chr3.3:64389997-64390016 None:intergenic 20.0%
!!! GATAATGTTTTTGTTTGTTA+AGG - chr3.3:64390356-64390375 None:intergenic 20.0%
! AAATCTCAGTCAATTTAATC+TGG + chr3.3:64390119-64390138 MS.gene35578:CDS 25.0%
!! ATCAAGAATTTGCTTGTTTA+GGG - chr3.3:64390311-64390330 None:intergenic 25.0%
!! TGCAAAATTTGCTTACATTT+GGG + chr3.3:64390516-64390535 MS.gene35578:CDS 25.0%
!! TTGATTATAGCTTTTTGTGA+TGG - chr3.3:64390160-64390179 None:intergenic 25.0%
!! TTGCAAAATTTGCTTACATT+TGG + chr3.3:64390515-64390534 MS.gene35578:CDS 25.0%
CTTGATGAGAATTACTCATT+AGG + chr3.3:64390325-64390344 MS.gene35578:CDS 30.0%
GAAAGTAACCATTTCCAAAA+GGG - chr3.3:64390702-64390721 None:intergenic 30.0%
TCAGTCAATTTAATCTGGTA+TGG + chr3.3:64390124-64390143 MS.gene35578:CDS 30.0%
TGAAAGTAACCATTTCCAAA+AGG - chr3.3:64390703-64390722 None:intergenic 30.0%
TGATGGAATGGTGATAAATT+TGG + chr3.3:64390648-64390667 MS.gene35578:CDS 30.0%
TTTGAGAAGGGATTGAAAAT+TGG - chr3.3:64389984-64390003 None:intergenic 30.0%
! AAATTTGCTTACATTTGGGT+TGG + chr3.3:64390520-64390539 MS.gene35578:CDS 30.0%
! ATTATCCAATTAGCTTCCAA+AGG + chr3.3:64390370-64390389 MS.gene35578:CDS 30.0%
! CATCAAGAATTTGCTTGTTT+AGG - chr3.3:64390312-64390331 None:intergenic 30.0%
! GAGATTTTACCATAGAGTAA+TGG - chr3.3:64390106-64390125 None:intergenic 30.0%
! TTCTGTAGAACGTTTTTGTA+TGG + chr3.3:64390438-64390457 MS.gene35578:CDS 30.0%
! TTTGGAAATGGTTACTTTCA+AGG + chr3.3:64390703-64390722 MS.gene35578:CDS 30.0%
!!! ATTTTTTCTTTGAGGTGAGA+TGG - chr3.3:64390270-64390289 None:intergenic 30.0%
!!! CTGTGTAGATTTTTTCTTTG+AGG - chr3.3:64390278-64390297 None:intergenic 30.0%
AATGGAATCCAGAAAAGGTA+AGG + chr3.3:64390492-64390511 MS.gene35578:CDS 35.0%
AGATGTTAGTACAACGTTGA+TGG - chr3.3:64390410-64390429 None:intergenic 35.0%
CCATTTCACCAACCAATTTA+CGG + chr3.3:64390580-64390599 MS.gene35578:CDS 35.0%
GTTGAGCAAGATAATGTAGA+AGG - chr3.3:64390895-64390914 None:intergenic 35.0%
TCTTCAATGGAATCCAGAAA+AGG + chr3.3:64390487-64390506 MS.gene35578:CDS 35.0%
TTCAATGCTCATGGTGATAA+TGG + chr3.3:64390838-64390857 MS.gene35578:CDS 35.0%
! TTTTGCAACCTTACCTTTTC+TGG - chr3.3:64390503-64390522 None:intergenic 35.0%
!! GAGATGGTAGTATTTTTCAG+TGG - chr3.3:64390254-64390273 None:intergenic 35.0%
!! TTGCTTGTTGATTCTACTAC+TGG + chr3.3:64390808-64390827 MS.gene35578:CDS 35.0%
!!! AATTTGGCTAGTCTTTTAGC+AGG + chr3.3:64390664-64390683 MS.gene35578:CDS 35.0%
ACACGGTTCTTCTTCTTCAA+TGG + chr3.3:64390474-64390493 MS.gene35578:CDS 40.0%
CAGCTTCTTCATTACCACAA+CGG + chr3.3:64390079-64390098 MS.gene35578:CDS 40.0%
GGAATCTTTGTGTTCACCTT+TGG - chr3.3:64390389-64390408 None:intergenic 40.0%
GTAGAAGGATCATACAAAGC+AGG - chr3.3:64390880-64390899 None:intergenic 40.0%
TGAAGATGGTGAAGAAAGTG+AGG - chr3.3:64390194-64390213 None:intergenic 40.0%
TGTCCTGGTGTTTATGGTAA+AGG + chr3.3:64390760-64390779 MS.gene35578:CDS 40.0%
TTGCACCAAACAACGATGTA+GGG + chr3.3:64390623-64390642 MS.gene35578:CDS 40.0%
TTTGTGGACCGTAAATTGGT+TGG - chr3.3:64390591-64390610 None:intergenic 40.0%
! GTTCACCTTTGGAAGCTAAT+TGG - chr3.3:64390378-64390397 None:intergenic 40.0%
!! AGTTTCTAGCAGCTGAAGAA+AGG - chr3.3:64390033-64390052 None:intergenic 40.0%
!! ATGCTCATGGTGATAATGGA+AGG + chr3.3:64390842-64390861 MS.gene35578:CDS 40.0%
!! CCGTAAATTGGTTGGTGAAA+TGG - chr3.3:64390583-64390602 None:intergenic 40.0%
!! GCAACAAACCCTTTTGGAAA+TGG + chr3.3:64390691-64390710 MS.gene35578:CDS 40.0%
!!! GGTTGGTTTGGTTTTGAAGA+TGG - chr3.3:64390208-64390227 None:intergenic 40.0%
AACAACGATGTAGGGCTTGA+TGG + chr3.3:64390631-64390650 MS.gene35578:CDS 45.0%
AACCTTCAGTTTCAGCATGG+TGG + chr3.3:64390224-64390243 MS.gene35578:CDS 45.0%
AACTCAGAAACACAGTGTCC+AGG + chr3.3:64390544-64390563 MS.gene35578:CDS 45.0%
ACCAACCTTCAGTTTCAGCA+TGG + chr3.3:64390221-64390240 MS.gene35578:CDS 45.0%
ACCATGCTGAAACTGAAGGT+TGG - chr3.3:64390225-64390244 None:intergenic 45.0%
ACTCAGAAACACAGTGTCCA+GGG + chr3.3:64390545-64390564 MS.gene35578:CDS 45.0%
AGCAAATCACCAGCATAACC+AGG - chr3.3:64390793-64390812 None:intergenic 45.0%
CACAACGGTCCATTACTCTA+TGG + chr3.3:64390094-64390113 MS.gene35578:CDS 45.0%
GCAAATCACCAGCATAACCA+GGG - chr3.3:64390792-64390811 None:intergenic 45.0%
GCACCTTTACCATAAACACC+AGG - chr3.3:64390766-64390785 None:intergenic 45.0%
GTTGCACCAAACAACGATGT+AGG + chr3.3:64390622-64390641 MS.gene35578:CDS 45.0%
TCAAGCCCTACATCGTTGTT+TGG - chr3.3:64390631-64390650 None:intergenic 45.0%
TCAGCTTGTCCTGGTGTTTA+TGG + chr3.3:64390754-64390773 MS.gene35578:CDS 45.0%
TTCCACCATGCTGAAACTGA+AGG - chr3.3:64390229-64390248 None:intergenic 45.0%
! CGTTTTTGTATGGACCGATG+TGG + chr3.3:64390448-64390467 MS.gene35578:CDS 45.0%
! GCTGAAACTGAAGGTTGGTT+TGG - chr3.3:64390220-64390239 None:intergenic 45.0%
! GGAACTGCAACAAACCCTTT+TGG + chr3.3:64390685-64390704 MS.gene35578:CDS 45.0%
! GGTGCTAGTTTCAATGCTCA+TGG + chr3.3:64390829-64390848 MS.gene35578:CDS 45.0%
!! CATAGAGTAATGGACCGTTG+TGG - chr3.3:64390096-64390115 None:intergenic 45.0%
!! TATGGTAAAGGTGCTTACCC+TGG + chr3.3:64390772-64390791 MS.gene35578:CDS 45.0%
!! TCGTTGTTTGGTGCAACCAA+TGG - chr3.3:64390619-64390638 None:intergenic 45.0%
AAGAACCGTGTGTGCCACAT+CGG - chr3.3:64390465-64390484 None:intergenic 50.0%
AATGGCCAAGCACATAACCC+TGG - chr3.3:64390565-64390584 None:intergenic 50.0%
AGTGTCCAGGGTTATGTGCT+TGG + chr3.3:64390557-64390576 MS.gene35578:CDS 50.0%
CAAGCTGAAGCAGCTTCAAG+AGG - chr3.3:64390742-64390761 None:intergenic 50.0%
CGATGTAGGGCTTGATGGAA+TGG + chr3.3:64390636-64390655 MS.gene35578:CDS 50.0%
GCGCTTTGTGGACCGTAAAT+TGG - chr3.3:64390595-64390614 None:intergenic 50.0%
GCTTCAAGAGGAGCTTCAGA+AGG - chr3.3:64390730-64390749 None:intergenic 50.0%
ATGGACCGATGTGGCACACA+CGG + chr3.3:64390457-64390476 MS.gene35578:CDS 55.0%
GAAGCTGCTTCAGCTTGTCC+TGG + chr3.3:64390745-64390764 MS.gene35578:CDS 55.0%
GCAACCAATGGTGCGCTTTG+TGG - chr3.3:64390607-64390626 None:intergenic 55.0%
GGTGCTTACCCTGGTTATGC+TGG + chr3.3:64390781-64390800 MS.gene35578:CDS 55.0%
CGGTCCACAAAGCGCACCAT+TGG + chr3.3:64390600-64390619 MS.gene35578:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 64389974 64390924 64389974 ID=MS.gene35578
chr3.3 mRNA 64389974 64390924 64389974 ID=MS.gene35578.t1;Parent=MS.gene35578
chr3.3 exon 64389974 64390924 64389974 ID=MS.gene35578.t1.exon1;Parent=MS.gene35578.t1
chr3.3 CDS 64389974 64390924 64389974 ID=cds.MS.gene35578.t1;Parent=MS.gene35578.t1
Gene Sequence

>MS.gene35578

ATGGCTTCCAATTTTCAATCCCTTCTCAAATTTTTTCTTCTTATCTCAATTGTTTACCTTTCTTCAGCTGCTAGAAACTTGAACGAGCTTGTTCAAGATCAATCTCAGCTTCTTCATTACCACAACGGTCCATTACTCTATGGTAAAATCTCAGTCAATTTAATCTGGTATGGTAACTTTAAACCATCACAAAAAGCTATAATCAATGATTTTTTCACCTCACTTTCTTCACCATCTTCAAAACCAAACCAACCTTCAGTTTCAGCATGGTGGAAAACCACTGAAAAATACTACCATCTCACCTCAAAGAAAAAATCTACACAGCTTTCACTTTCCCTAAACAAGCAAATTCTTGATGAGAATTACTCATTAGGAAAATCCTTAACAAACAAAAACATTATCCAATTAGCTTCCAAAGGTGAACACAAAGATTCCATCAACGTTGTACTAACATCTGCTGATGTTTCTGTAGAACGTTTTTGTATGGACCGATGTGGCACACACGGTTCTTCTTCTTCAATGGAATCCAGAAAAGGTAAGGTTGCAAAATTTGCTTACATTTGGGTTGGTAACTCAGAAACACAGTGTCCAGGGTTATGTGCTTGGCCATTTCACCAACCAATTTACGGTCCACAAAGCGCACCATTGGTTGCACCAAACAACGATGTAGGGCTTGATGGAATGGTGATAAATTTGGCTAGTCTTTTAGCAGGAACTGCAACAAACCCTTTTGGAAATGGTTACTTTCAAGGTCCTTCTGAAGCTCCTCTTGAAGCTGCTTCAGCTTGTCCTGGTGTTTATGGTAAAGGTGCTTACCCTGGTTATGCTGGTGATTTGCTTGTTGATTCTACTACTGGTGCTAGTTTCAATGCTCATGGTGATAATGGAAGGAAGTATCTTCTTCCTGCTTTGTATGATCCTTCTACATTATCTTGCTCAACCCTTGTTTGA

Protein sequence

>MS.gene35578.t1

MASNFQSLLKFFLLISIVYLSSAARNLNELVQDQSQLLHYHNGPLLYGKISVNLIWYGNFKPSQKAIINDFFTSLSSPSSKPNQPSVSAWWKTTEKYYHLTSKKKSTQLSLSLNKQILDENYSLGKSLTNKNIIQLASKGEHKDSINVVLTSADVSVERFCMDRCGTHGSSSSMESRKGKVAKFAYIWVGNSETQCPGLCAWPFHQPIYGPQSAPLVAPNNDVGLDGMVINLASLLAGTATNPFGNGYFQGPSEAPLEAASACPGVYGKGAYPGYAGDLLVDSTTGASFNAHGDNGRKYLLPALYDPSTLSCSTLV