Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35632.t1 | KEH38081.1 | 96.1 | 308 | 10 | 1 | 1 | 308 | 1 | 306 | 2.40E-158 | 568.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35632.t1 | Q94A09 | 65.7 | 309 | 71 | 3 | 35 | 308 | 64 | 372 | 2.5e-103 | 376.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35632.t1 | A0A072V8X2 | 96.1 | 308 | 10 | 1 | 1 | 308 | 1 | 306 | 1.7e-158 | 568.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051699 | MS.gene35632 | 0.807518 | 4.86E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35632.t1 | MTR_2g460880 | 94.481 | 308 | 15 | 1 | 1 | 308 | 1 | 306 | 0.0 | 580 |
| MS.gene35632.t1 | MTR_2g460880 | 94.481 | 308 | 15 | 1 | 1 | 308 | 1 | 306 | 0.0 | 580 |
| MS.gene35632.t1 | MTR_2g460880 | 94.463 | 307 | 15 | 1 | 1 | 307 | 1 | 305 | 0.0 | 579 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35632.t1 | AT1G36310 | 63.663 | 344 | 89 | 5 | 1 | 308 | 29 | 372 | 4.23e-132 | 381 |
| MS.gene35632.t1 | AT1G36310 | 63.663 | 344 | 89 | 5 | 1 | 308 | 29 | 372 | 4.23e-132 | 381 |
Find 65 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGGGAATGGAAAGTATTT+AGG | 0.153553 | 3.3:-63630060 | MS.gene35632:CDS |
| ATTGGTTAGGGTTGTTAAAA+AGG | 0.210227 | 3.3:-63629839 | MS.gene35632:CDS |
| TTGCGATGAGTCCGAAGTTT+TGG | 0.246310 | 3.3:-63629614 | MS.gene35632:CDS |
| CTTCCTTATAGAACTGGTTT+TGG | 0.252508 | 3.3:-63629934 | MS.gene35632:CDS |
| GTGGGAATGGAAAGTATTTA+GGG | 0.290355 | 3.3:-63630059 | MS.gene35632:CDS |
| AAACCACTCTCCTCACTTTC+CGG | 0.306191 | 3.3:+63629658 | None:intergenic |
| TCTGCTCATGCTTTGGCTAC+TGG | 0.313548 | 3.3:-63629499 | MS.gene35632:CDS |
| GCGAAAAGCGATTGAAGAAT+TGG | 0.341686 | 3.3:-63629857 | MS.gene35632:CDS |
| TGCTACTAAAGATGATATAA+AGG | 0.351714 | 3.3:-63629473 | MS.gene35632:CDS |
| AAGCGATTGAAGAATTGGTT+AGG | 0.364631 | 3.3:-63629852 | MS.gene35632:CDS |
| TCACCAAAACCAGTTCTATA+AGG | 0.375130 | 3.3:+63629931 | None:intergenic |
| ATTAAGATTTGTTTAGATAG+AGG | 0.380488 | 3.3:-63629988 | MS.gene35632:CDS |
| GTTAATCTTCCTTATAGAAC+TGG | 0.382656 | 3.3:-63629940 | MS.gene35632:CDS |
| TGTAGAAATTCAGCAACTTT+CGG | 0.385442 | 3.3:+63630120 | None:intergenic |
| GTTCGACCCGTTATGCGAAA+TGG | 0.390668 | 3.3:-63630143 | MS.gene35632:CDS |
| GAAAGTGAGGAGAGTGGTTT+GGG | 0.396880 | 3.3:-63629655 | MS.gene35632:CDS |
| GTTGTCGTTGGAAAGTATTC+CGG | 0.398684 | 3.3:-63629677 | MS.gene35632:CDS |
| CGAGTAGCACGTGGACTTCC+CGG | 0.400143 | 3.3:+63629712 | None:intergenic |
| TGGTGCTTCTGCTCATGCTT+TGG | 0.413666 | 3.3:-63629506 | MS.gene35632:CDS |
| CGGGAAGTCCACGTGCTACT+CGG | 0.424013 | 3.3:-63629711 | MS.gene35632:CDS |
| GGTGTCTTCTTCGTTGTCGT+TGG | 0.424077 | 3.3:-63629689 | MS.gene35632:CDS |
| TTTCGGCCATTTCGCATAAC+GGG | 0.431695 | 3.3:+63630137 | None:intergenic |
| GGAAAGTGAGGAGAGTGGTT+TGG | 0.441010 | 3.3:-63629656 | MS.gene35632:CDS |
| CTGACTTCAGCGCGATGATA+AGG | 0.451316 | 3.3:+63629529 | None:intergenic |
| CTTTCGGCCATTTCGCATAA+CGG | 0.455080 | 3.3:+63630136 | None:intergenic |
| TTTCTACAAAATTTGCATCC+AGG | 0.461897 | 3.3:-63630105 | MS.gene35632:CDS |
| ATGAGGCGTTGGTTAAGAAA+TGG | 0.464016 | 3.3:-63629774 | MS.gene35632:CDS |
| ATTTGAGTACTGAGAATAGA+AGG | 0.466020 | 3.3:-63629879 | MS.gene35632:CDS |
| GCTACTAAAGATGATATAAA+GGG | 0.466972 | 3.3:-63629472 | MS.gene35632:CDS |
| TGTTGAAGAATGGCAGGGAC+CGG | 0.479768 | 3.3:-63629731 | MS.gene35632:CDS |
| CATTCCCACATCCTGCATCC+AGG | 0.488324 | 3.3:+63630073 | None:intergenic |
| AAACAAATCTTAATCAATGA+AGG | 0.489573 | 3.3:+63629997 | None:intergenic |
| AAAGAATGTGAAGAATCAAC+AGG | 0.490219 | 3.3:-63629575 | MS.gene35632:CDS |
| AGCGATTGAAGAATTGGTTA+GGG | 0.495251 | 3.3:-63629851 | MS.gene35632:CDS |
| TGATGAATTGTGTGTGATGT+AGG | 0.502313 | 3.3:+63630287 | None:intergenic |
| GCATCCAGGATCAATGATCC+TGG | 0.507454 | 3.3:+63630087 | None:intergenic |
| GAAGTATGTTGAAGAATGGC+AGG | 0.508849 | 3.3:-63629737 | MS.gene35632:CDS |
| GCATCCAGGATCATTGATCC+TGG | 0.514150 | 3.3:-63630091 | MS.gene35632:CDS |
| GGATCATTGATCCTGGATGC+AGG | 0.515522 | 3.3:-63630084 | MS.gene35632:CDS |
| GGGAAGTCCACGTGCTACTC+GGG | 0.516924 | 3.3:-63629710 | MS.gene35632:CDS |
| GGAGAGTGGTTTGGGTGGTG+AGG | 0.519039 | 3.3:-63629647 | MS.gene35632:CDS |
| TTAGCGAAGGCGAGCTTGAA+AGG | 0.519347 | 3.3:-63629417 | MS.gene35632:CDS |
| GGCTGTTGAACAAGAGGATG+AGG | 0.522357 | 3.3:-63629791 | MS.gene35632:CDS |
| TTGATCCTGGATGCAGGATG+TGG | 0.522669 | 3.3:-63630078 | MS.gene35632:CDS |
| CTTATAGATGATACTACAGC+TGG | 0.534238 | 3.3:+63630237 | None:intergenic |
| CTTCAAATCTCCCAAAACTT+CGG | 0.542427 | 3.3:+63629603 | None:intergenic |
| AACGGGTCGAACTAAAATGT+GGG | 0.551768 | 3.3:+63630154 | None:intergenic |
| CTGGATGCAGGATGTGGGAA+TGG | 0.555129 | 3.3:-63630072 | MS.gene35632:CDS |
| TCAACATACTTCTCAGCAAG+CGG | 0.560715 | 3.3:+63629748 | None:intergenic |
| TGAACAAGAGGATGAGGCGT+TGG | 0.562919 | 3.3:-63629785 | MS.gene35632:CDS |
| GTTGAAGAATGGCAGGGACC+GGG | 0.566489 | 3.3:-63629730 | MS.gene35632:CDS |
| CTGAGAAGTATGTTGAAGAA+TGG | 0.575749 | 3.3:-63629741 | MS.gene35632:CDS |
| GATGAATTGTGTGTGATGTA+GGG | 0.581198 | 3.3:+63630288 | None:intergenic |
| TAACGGGTCGAACTAAAATG+TGG | 0.594991 | 3.3:+63630153 | None:intergenic |
| AGTGAGGAGAGTGGTTTGGG+TGG | 0.597452 | 3.3:-63629652 | MS.gene35632:CDS |
| CGATGATAAGGTAAATGCCA+AGG | 0.604941 | 3.3:+63629541 | None:intergenic |
| GGAAAGTATTCCGGAAAGTG+AGG | 0.620911 | 3.3:-63629668 | MS.gene35632:CDS |
| TGTTTGGGCTGTTGAACAAG+AGG | 0.638871 | 3.3:-63629797 | MS.gene35632:CDS |
| TATCATCGCGCTGAAGTCAG+TGG | 0.641644 | 3.3:-63629526 | MS.gene35632:CDS |
| TGATCCTGGATGCAGGATGT+GGG | 0.666318 | 3.3:-63630077 | MS.gene35632:CDS |
| AAGTATGTTGAAGAATGGCA+GGG | 0.670774 | 3.3:-63629736 | MS.gene35632:CDS |
| GAAGACACCCGAGTAGCACG+TGG | 0.670935 | 3.3:+63629703 | None:intergenic |
| ATTCCGGAAAGTGAGGAGAG+TGG | 0.676753 | 3.3:-63629661 | MS.gene35632:CDS |
| ACGGGTCGAACTAAAATGTG+GGG | 0.683186 | 3.3:+63630155 | None:intergenic |
| GGGCAATTGCATCATAAACA+TGG | 0.702606 | 3.3:+63630175 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAAGATTGTTTTTTTAT+AGG | - | chr3.3:63629699-63629718 | MS.gene35632:CDS | 10.0% |
| !! | AAACAAATCTTAATCAATGA+AGG | + | chr3.3:63629738-63629757 | None:intergenic | 20.0% |
| !!! | ACATACTTTTTTTCAATTTC+AGG | + | chr3.3:63629534-63629553 | None:intergenic | 20.0% |
| !!! | ATACTTTTTTTCAATTTCAG+GGG | + | chr3.3:63629532-63629551 | None:intergenic | 20.0% |
| !!! | ATTAAGATTTGTTTAGATAG+AGG | - | chr3.3:63629744-63629763 | MS.gene35632:CDS | 20.0% |
| !!! | CATACTTTTTTTCAATTTCA+GGG | + | chr3.3:63629533-63629552 | None:intergenic | 20.0% |
| ! | GCTACTAAAGATGATATAAA+GGG | - | chr3.3:63630260-63630279 | MS.gene35632:CDS | 25.0% |
| ! | TGCTACTAAAGATGATATAA+AGG | - | chr3.3:63630259-63630278 | MS.gene35632:CDS | 25.0% |
| !!! | TATTATCATGTTTTTAGCGA+AGG | - | chr3.3:63630302-63630321 | MS.gene35632:CDS | 25.0% |
| AAAGAATGTGAAGAATCAAC+AGG | - | chr3.3:63630157-63630176 | MS.gene35632:CDS | 30.0% | |
| ATTGGTTAGGGTTGTTAAAA+AGG | - | chr3.3:63629893-63629912 | MS.gene35632:CDS | 30.0% | |
| GTTAATCTTCCTTATAGAAC+TGG | - | chr3.3:63629792-63629811 | MS.gene35632:CDS | 30.0% | |
| TGTAGAAATTCAGCAACTTT+CGG | + | chr3.3:63629615-63629634 | None:intergenic | 30.0% | |
| TTGGTTAGGGTTGTTAAAAA+GGG | - | chr3.3:63629894-63629913 | MS.gene35632:CDS | 30.0% | |
| TTTCTACAAAATTTGCATCC+AGG | - | chr3.3:63629627-63629646 | MS.gene35632:CDS | 30.0% | |
| !! | ATTTGAGTACTGAGAATAGA+AGG | - | chr3.3:63629853-63629872 | MS.gene35632:CDS | 30.0% |
| !!! | GCTTGTTTTGATTACTGTTT+GGG | - | chr3.3:63629920-63629939 | MS.gene35632:CDS | 30.0% |
| AAGCGATTGAAGAATTGGTT+AGG | - | chr3.3:63629880-63629899 | MS.gene35632:CDS | 35.0% | |
| AAGTATGTTGAAGAATGGCA+GGG | - | chr3.3:63629996-63630015 | MS.gene35632:CDS | 35.0% | |
| AGCGATTGAAGAATTGGTTA+GGG | - | chr3.3:63629881-63629900 | MS.gene35632:CDS | 35.0% | |
| CTTATAGATGATACTACAGC+TGG | + | chr3.3:63629498-63629517 | None:intergenic | 35.0% | |
| CTTCAAATCTCCCAAAACTT+CGG | + | chr3.3:63630132-63630151 | None:intergenic | 35.0% | |
| GATGAATTGTGTGTGATGTA+GGG | + | chr3.3:63629447-63629466 | None:intergenic | 35.0% | |
| TCACCAAAACCAGTTCTATA+AGG | + | chr3.3:63629804-63629823 | None:intergenic | 35.0% | |
| TGATGAATTGTGTGTGATGT+AGG | + | chr3.3:63629448-63629467 | None:intergenic | 35.0% | |
| TTAGGGTTGTTAAAAAGGGT+GGG | - | chr3.3:63629898-63629917 | MS.gene35632:CDS | 35.0% | |
| ! | CTGAGAAGTATGTTGAAGAA+TGG | - | chr3.3:63629991-63630010 | MS.gene35632:CDS | 35.0% |
| !! | CTTCCTTATAGAACTGGTTT+TGG | - | chr3.3:63629798-63629817 | MS.gene35632:CDS | 35.0% |
| !! | GTGGGAATGGAAAGTATTTA+GGG | - | chr3.3:63629673-63629692 | MS.gene35632:CDS | 35.0% |
| !! | TGTGGGAATGGAAAGTATTT+AGG | - | chr3.3:63629672-63629691 | MS.gene35632:CDS | 35.0% |
| !!! | AACAGGAGTATTTTGTTCCT+TGG | - | chr3.3:63630174-63630193 | MS.gene35632:CDS | 35.0% |
| !!! | CTTTTTTTCAATTTCAGGGG+TGG | + | chr3.3:63629529-63629548 | None:intergenic | 35.0% |
| !!! | GGCTTGTTTTGATTACTGTT+TGG | - | chr3.3:63629919-63629938 | MS.gene35632:CDS | 35.0% |
| AACGGGTCGAACTAAAATGT+GGG | + | chr3.3:63629581-63629600 | None:intergenic | 40.0% | |
| CGATGATAAGGTAAATGCCA+AGG | + | chr3.3:63630194-63630213 | None:intergenic | 40.0% | |
| GAAGTATGTTGAAGAATGGC+AGG | - | chr3.3:63629995-63630014 | MS.gene35632:CDS | 40.0% | |
| GCGAAAAGCGATTGAAGAAT+TGG | - | chr3.3:63629875-63629894 | MS.gene35632:CDS | 40.0% | |
| GGGCAATTGCATCATAAACA+TGG | + | chr3.3:63629560-63629579 | None:intergenic | 40.0% | |
| GTTAGGGTTGTTAAAAAGGG+TGG | - | chr3.3:63629897-63629916 | MS.gene35632:CDS | 40.0% | |
| TCAACATACTTCTCAGCAAG+CGG | + | chr3.3:63629987-63630006 | None:intergenic | 40.0% | |
| ! | TAACGGGTCGAACTAAAATG+TGG | + | chr3.3:63629582-63629601 | None:intergenic | 40.0% |
| !! | ATGAGGCGTTGGTTAAGAAA+TGG | - | chr3.3:63629958-63629977 | MS.gene35632:CDS | 40.0% |
| !! | GTTGTCGTTGGAAAGTATTC+CGG | - | chr3.3:63630055-63630074 | MS.gene35632:CDS | 40.0% |
| !!! | CGAAGTTTTGGGAGATTTGA+AGG | - | chr3.3:63630130-63630149 | MS.gene35632:CDS | 40.0% |
| AAACCACTCTCCTCACTTTC+CGG | + | chr3.3:63630077-63630096 | None:intergenic | 45.0% | |
| ACGGGTCGAACTAAAATGTG+GGG | + | chr3.3:63629580-63629599 | None:intergenic | 45.0% | |
| CTTTCGGCCATTTCGCATAA+CGG | + | chr3.3:63629599-63629618 | None:intergenic | 45.0% | |
| GAAAGTGAGGAGAGTGGTTT+GGG | - | chr3.3:63630077-63630096 | MS.gene35632:CDS | 45.0% | |
| TGTTTGGGCTGTTGAACAAG+AGG | - | chr3.3:63629935-63629954 | MS.gene35632:CDS | 45.0% | |
| TTTCGGCCATTTCGCATAAC+GGG | + | chr3.3:63629598-63629617 | None:intergenic | 45.0% | |
| ! | TGCGATGAGTCCGAAGTTTT+GGG | - | chr3.3:63630119-63630138 | MS.gene35632:CDS | 45.0% |
| ! | TTGCGATGAGTCCGAAGTTT+TGG | - | chr3.3:63630118-63630137 | MS.gene35632:CDS | 45.0% |
| !! | GGAAAGTATTCCGGAAAGTG+AGG | - | chr3.3:63630064-63630083 | MS.gene35632:CDS | 45.0% |
| ATTCCGGAAAGTGAGGAGAG+TGG | - | chr3.3:63630071-63630090 | MS.gene35632:CDS | 50.0% | |
| CTGACTTCAGCGCGATGATA+AGG | + | chr3.3:63630206-63630225 | None:intergenic | 50.0% | |
| GCATCCAGGATCAATGATCC+TGG | + | chr3.3:63629648-63629667 | None:intergenic | 50.0% | |
| GCATCCAGGATCATTGATCC+TGG | - | chr3.3:63629641-63629660 | MS.gene35632:CDS | 50.0% | |
| GGAAAGTGAGGAGAGTGGTT+TGG | - | chr3.3:63630076-63630095 | MS.gene35632:CDS | 50.0% | |
| GGATCATTGATCCTGGATGC+AGG | - | chr3.3:63629648-63629667 | MS.gene35632:CDS | 50.0% | |
| GGCTGTTGAACAAGAGGATG+AGG | - | chr3.3:63629941-63629960 | MS.gene35632:CDS | 50.0% | |
| GGTGTCTTCTTCGTTGTCGT+TGG | - | chr3.3:63630043-63630062 | MS.gene35632:CDS | 50.0% | |
| GTTCGACCCGTTATGCGAAA+TGG | - | chr3.3:63629589-63629608 | MS.gene35632:CDS | 50.0% | |
| TATCATCGCGCTGAAGTCAG+TGG | - | chr3.3:63630206-63630225 | MS.gene35632:CDS | 50.0% | |
| TCTGCTCATGCTTTGGCTAC+TGG | - | chr3.3:63630233-63630252 | MS.gene35632:CDS | 50.0% | |
| TGAACAAGAGGATGAGGCGT+TGG | - | chr3.3:63629947-63629966 | MS.gene35632:CDS | 50.0% | |
| TGATCCTGGATGCAGGATGT+GGG | - | chr3.3:63629655-63629674 | MS.gene35632:CDS | 50.0% | |
| TGGTGCTTCTGCTCATGCTT+TGG | - | chr3.3:63630226-63630245 | MS.gene35632:CDS | 50.0% | |
| TGTTGAAGAATGGCAGGGAC+CGG | - | chr3.3:63630001-63630020 | MS.gene35632:CDS | 50.0% | |
| TTAGCGAAGGCGAGCTTGAA+AGG | - | chr3.3:63630315-63630334 | MS.gene35632:CDS | 50.0% | |
| TTGATCCTGGATGCAGGATG+TGG | - | chr3.3:63629654-63629673 | MS.gene35632:CDS | 50.0% | |
| CATTCCCACATCCTGCATCC+AGG | + | chr3.3:63629662-63629681 | None:intergenic | 55.0% | |
| CTGGATGCAGGATGTGGGAA+TGG | - | chr3.3:63629660-63629679 | MS.gene35632:CDS | 55.0% | |
| GTTGAAGAATGGCAGGGACC+GGG | - | chr3.3:63630002-63630021 | MS.gene35632:CDS | 55.0% | |
| ! | AGTGAGGAGAGTGGTTTGGG+TGG | - | chr3.3:63630080-63630099 | MS.gene35632:CDS | 55.0% |
| CGAGTAGCACGTGGACTTCC+CGG | + | chr3.3:63630023-63630042 | None:intergenic | 60.0% | |
| GAAGACACCCGAGTAGCACG+TGG | + | chr3.3:63630032-63630051 | None:intergenic | 60.0% | |
| ! | CGGGAAGTCCACGTGCTACT+CGG | - | chr3.3:63630021-63630040 | MS.gene35632:CDS | 60.0% |
| ! | GGAGAGTGGTTTGGGTGGTG+AGG | - | chr3.3:63630085-63630104 | MS.gene35632:CDS | 60.0% |
| ! | GGGAAGTCCACGTGCTACTC+GGG | - | chr3.3:63630022-63630041 | MS.gene35632:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 63629414 | 63630340 | 63629414 | ID=MS.gene35632 |
| chr3.3 | mRNA | 63629414 | 63630340 | 63629414 | ID=MS.gene35632.t1;Parent=MS.gene35632 |
| chr3.3 | exon | 63629414 | 63630340 | 63629414 | ID=MS.gene35632.t1.exon1;Parent=MS.gene35632.t1 |
| chr3.3 | CDS | 63629414 | 63630340 | 63629414 | ID=cds.MS.gene35632.t1;Parent=MS.gene35632.t1 |
| Gene Sequence |
| Protein sequence |
