AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35681

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAATCAGTTTGCCATTT+TGG 0.024477 2.1:-14553027 None:intergenic
AACTGTTCAACAATGCATTT+TGG 0.163705 2.1:+14553216 MS.gene35681:CDS
TTTCGATGAAGATGAAATTT+TGG 0.207548 2.1:+14551873 MS.gene35681:CDS
CTTCTTGCGCAGTGGATTAA+TGG 0.211642 2.1:-14551647 None:intergenic
GATGCAATGAACAAATTAAT+TGG 0.217137 2.1:+14550344 MS.gene35681:CDS
TGATTCAAAGCTGACTAAAT+TGG 0.235627 2.1:+14550733 MS.gene35681:CDS
AGATCAAGAAATTGCTAGTT+TGG 0.267521 2.1:+14553192 MS.gene35681:CDS
GAAGAATAGTGAAGATTCTT+TGG 0.305415 2.1:+14551801 MS.gene35681:CDS
TTCTTGCGCAGTGGATTAAT+GGG 0.320277 2.1:-14551646 None:intergenic
TGCATCAAGCTCTATCTCTT+TGG 0.335082 2.1:-14550327 None:intergenic
ACAGTCTGAGGTTCTTAAAA+AGG 0.339045 2.1:+14553249 MS.gene35681:CDS
GTTCCTCTTCCTTCCTTTCA+CGG 0.342021 2.1:-14550256 None:intergenic
TATTTGATGGAGAACTTGAT+TGG 0.352613 2.1:+14550134 MS.gene35681:CDS
TTGCAAGAACACATCCAAAA+TGG 0.376034 2.1:+14553013 MS.gene35681:CDS
AATTTAGTCAGCTTTGAATC+AGG 0.377766 2.1:-14550731 None:intergenic
CAGAAGAACCTTGATTTCAA+TGG 0.380322 2.1:-14553074 None:intergenic
GAAGATTCTTTGGCGGTCCT+TGG 0.394702 2.1:+14551811 MS.gene35681:CDS
TGAAGAGCAGCTCTATTTGA+TGG 0.413270 2.1:+14550121 MS.gene35681:CDS
TTGATTTGAAAGCTTACATA+AGG 0.423407 2.1:+14551779 MS.gene35681:CDS
TGGTGGAACAAGTTTGCAAT+TGG 0.423753 2.1:+14551534 MS.gene35681:CDS
ACTAGCAATTTCTTGATCTC+TGG 0.437247 2.1:-14553188 None:intergenic
CAGCTTTGAATCAGGATCCT+TGG 0.440098 2.1:-14550723 None:intergenic
ACATGCTGAAATGAAGGAAT+TGG 0.442708 2.1:+14550406 MS.gene35681:CDS
AACCTTGATTTCAATGGAGT+TGG 0.448151 2.1:-14553068 None:intergenic
GAAGAAGCAACTTGAGGGTC+AGG 0.452602 2.1:+14550436 MS.gene35681:CDS
CTTGAGCTACAAGCACATAA+AGG 0.454488 2.1:+14553289 MS.gene35681:CDS
GAAGGAGTTTCAATTAAAAG+AGG 0.459023 2.1:+14550544 MS.gene35681:CDS
TGCTCAGCACAAGATAGATA+TGG 0.459143 2.1:+14551912 MS.gene35681:CDS
AACCAACTCCATTGAAATCA+AGG 0.472960 2.1:+14553066 MS.gene35681:CDS
TCAAGAGAGAAGCGATTTGA+GGG 0.483952 2.1:+14550515 MS.gene35681:CDS
TGAAGAACGTCAGAAAGAAA+TGG 0.486974 2.1:+14550658 MS.gene35681:CDS
GATTGATTACCTGTCAACTT+AGG 0.488310 2.1:-14550773 None:intergenic
ATTCAGTTGTGCATATAGCT+TGG 0.490539 2.1:+14552964 MS.gene35681:CDS
GAATGGACATGCTGAAATGA+AGG 0.492084 2.1:+14550400 MS.gene35681:CDS
ATGGAGATGTTTCAGACCCT+GGG 0.497840 2.1:+14551931 MS.gene35681:CDS
GCGATTTGAGGGACAAATGA+AGG 0.500270 2.1:+14550526 MS.gene35681:CDS
CTTACACTATCCTCAACATT+CGG 0.500565 2.1:-14551736 None:intergenic
TAAATTGGATGGACAAATGA+AGG 0.500873 2.1:+14550748 MS.gene35681:CDS
GGAAATTAATTCAAAGGTGG+AGG 0.501415 2.1:+14550589 MS.gene35681:CDS
TATGGAGATGTTTCAGACCC+TGG 0.504944 2.1:+14551930 MS.gene35681:CDS
ATATGCAGTTGCATCTTACA+TGG 0.508808 2.1:+14551489 MS.gene35681:intron
GCTTCGTCAAGTCATTGATA+AGG 0.509491 2.1:+14550232 MS.gene35681:CDS
AAGAAATGGTGAATGAGCTC+AGG 0.513182 2.1:+14550672 MS.gene35681:CDS
ATCAAGAGAGAAGCGATTTG+AGG 0.513748 2.1:+14550514 MS.gene35681:CDS
GGAATTTGAAGTCAGAGTGA+AGG 0.514766 2.1:+14550568 MS.gene35681:CDS
CATTAATCCACTGCGCAAGA+AGG 0.523181 2.1:+14551648 MS.gene35681:CDS
CGATTAAGTCATACCATTGG+TGG 0.525094 2.1:+14551517 MS.gene35681:CDS
TCAATGAAGAAGCAACTTGA+GGG 0.527129 2.1:+14550431 MS.gene35681:CDS
ATGAAGGAGCCTAAGTTGAC+AGG 0.531788 2.1:+14550764 MS.gene35681:CDS
GGAGTTTCAATTAAAAGAGG+AGG 0.531791 2.1:+14550547 MS.gene35681:CDS
GCAGTTTGAAAGACAAGCCA+AGG 0.542581 2.1:+14550706 MS.gene35681:CDS
TTGTCTCCCTTCTTGCGCAG+TGG 0.549977 2.1:-14551655 None:intergenic
AGTGAAGGAAATTAATTCAA+AGG 0.550031 2.1:+14550583 MS.gene35681:CDS
TTGCAAACTTGTTCCACCAA+TGG 0.556249 2.1:-14551530 None:intergenic
GATAGAACTTAATCAAGTCA+AGG 0.560272 2.1:+14550187 MS.gene35681:CDS
TGATAAGGACCGTGAAAGGA+AGG 0.560715 2.1:+14550247 MS.gene35681:CDS
ATTAATCCACTGCGCAAGAA+GGG 0.561956 2.1:+14551649 MS.gene35681:CDS
GGATTGCAAACTACAGTCTG+AGG 0.567148 2.1:+14553237 MS.gene35681:CDS
GGAACTCGAAGATCAAGTTA+AGG 0.570629 2.1:+14550610 MS.gene35681:CDS
GAATAGTGAAGATTCTTTGG+CGG 0.573764 2.1:+14551804 MS.gene35681:CDS
TCAAAGCTGACTAAATTGGA+TGG 0.579416 2.1:+14550737 MS.gene35681:CDS
CTCAATGAAGAAGCAACTTG+AGG 0.581176 2.1:+14550430 MS.gene35681:CDS
GATCGATTAAGTCATACCAT+TGG 0.581766 2.1:+14551514 MS.gene35681:CDS
ATAGAACTTAATCAAGTCAA+GGG 0.583347 2.1:+14550188 MS.gene35681:CDS
TGAAGTTTGTGACAAAGTGC+AGG 0.585398 2.1:+14550061 MS.gene35681:CDS
GGACCGTGAAAGGAAGGAAG+AGG 0.585661 2.1:+14550253 MS.gene35681:CDS
GAAGGAAATTAATTCAAAGG+TGG 0.597705 2.1:+14550586 MS.gene35681:CDS
TTGTTGAGAATCTTATCAAG+AGG 0.606243 2.1:+14552918 MS.gene35681:CDS
TCATTGATAAGGACCGTGAA+AGG 0.608463 2.1:+14550243 MS.gene35681:CDS
GAGAACCTCACCGAATGTTG+AGG 0.611156 2.1:+14551726 MS.gene35681:CDS
AGAGCGCAAGATAGAGCTCA+AGG 0.612522 2.1:+14550157 MS.gene35681:CDS
ACAAAAGCAGTAGAAATCCA+AGG 0.630235 2.1:-14551828 None:intergenic
GTTGTTCAAGCAGAAAGATG+CGG 0.658407 2.1:-14551699 None:intergenic
ACTGTTGCAGTTGAGTCACA+AGG 0.688288 2.1:+14551571 MS.gene35681:CDS
ACTATCCTCAACATTCGGTG+AGG 0.689914 2.1:-14551731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGCTTAATTATAATATTA+TGG + chr2.1:14551108-14551127 MS.gene35681:intron 15.0%
!!! AAAAATTGATGAATGTATTT+TGG + chr2.1:14550295-14550314 MS.gene35681:CDS 15.0%
!!! AAATACATTCATCAATTTTT+TGG - chr2.1:14550295-14550314 None:intergenic 15.0%
!!! AATACATTCATCAATTTTTT+GGG - chr2.1:14550294-14550313 None:intergenic 15.0%
!! AAATTGAACTGATATGTAAT+TGG + chr2.1:14552661-14552680 MS.gene35681:intron 20.0%
!! AAGGGATAAATTAAAGATAA+TGG + chr2.1:14552538-14552557 MS.gene35681:intron 20.0%
!! ATATGAATAAGAACATTTAC+CGG - chr2.1:14550837-14550856 None:intergenic 20.0%
!! ATTAAGAAAATATATTGACG+GGG + chr2.1:14550802-14550821 MS.gene35681:intron 20.0%
!! CATTAAGAAAATATATTGAC+GGG + chr2.1:14550801-14550820 MS.gene35681:intron 20.0%
!! GCATTAAGAAAATATATTGA+CGG + chr2.1:14550800-14550819 MS.gene35681:intron 20.0%
!! TATCAAAACATGAAGATAAA+AGG - chr2.1:14552054-14552073 None:intergenic 20.0%
!!! AGATTTTTGTTGTTGTTAAT+TGG + chr2.1:14552854-14552873 MS.gene35681:intron 20.0%
!!! ATGAATCCTTTTAAGAAAAT+AGG + chr2.1:14552566-14552585 MS.gene35681:intron 20.0%
!!! ATTTTTTTTATTCATGGCTA+AGG + chr2.1:14551089-14551108 MS.gene35681:intron 20.0%
!!! TAGTTTTTCTTTTGCTAAAA+TGG + chr2.1:14552491-14552510 MS.gene35681:intron 20.0%
!!! TCTTGCATTTTTTTTATTCA+TGG + chr2.1:14551083-14551102 MS.gene35681:intron 20.0%
!!! TGTTTTGATATGAATAAATG+TGG - chr2.1:14552385-14552404 None:intergenic 20.0%
!!! TTACCCATTTTATCATTTAT+GGG + chr2.1:14552226-14552245 MS.gene35681:intron 20.0%
!!! TTTACCCATTTTATCATTTA+TGG + chr2.1:14552225-14552244 MS.gene35681:intron 20.0%
!!! TTTTTCAAGTATATTGCAAA+TGG + chr2.1:14551170-14551189 MS.gene35681:intron 20.0%
! AAAAACTGAAGAATGTGTTA+AGG + chr2.1:14550085-14550104 MS.gene35681:CDS 25.0%
! AGAAAAATTAGAGGCTAAAA+AGG + chr2.1:14550376-14550395 MS.gene35681:CDS 25.0%
! AGAGATTCATAAAATGTTCA+AGG - chr2.1:14551049-14551068 None:intergenic 25.0%
! AGTGAAGGAAATTAATTCAA+AGG + chr2.1:14550583-14550602 MS.gene35681:CDS 25.0%
! ATAGAACTTAATCAAGTCAA+GGG + chr2.1:14550188-14550207 MS.gene35681:CDS 25.0%
! ATCCTAACTGTAGATAATAA+TGG - chr2.1:14552134-14552153 None:intergenic 25.0%
! ATTACTATGTATTTGAACTC+AGG + chr2.1:14553159-14553178 MS.gene35681:intron 25.0%
! ATTCATGATCAAAGATTCAA+AGG + chr2.1:14552169-14552188 MS.gene35681:intron 25.0%
! GAGATTATGATATTAGTTGT+TGG + chr2.1:14552634-14552653 MS.gene35681:intron 25.0%
! GAGATTCATAAAATGTTCAA+GGG - chr2.1:14551048-14551067 None:intergenic 25.0%
! GATGCAATGAACAAATTAAT+TGG + chr2.1:14550344-14550363 MS.gene35681:CDS 25.0%
!! AGTTGTCCTATTTTCTTAAA+AGG - chr2.1:14552575-14552594 None:intergenic 25.0%
!! CATCTCTTCTATTTTCTAAA+TGG + chr2.1:14552795-14552814 MS.gene35681:intron 25.0%
!! GAGTTCATTGATTAATAAAC+AGG - chr2.1:14551265-14551284 None:intergenic 25.0%
!! TACCCATTTTATCATTTATG+GGG + chr2.1:14552227-14552246 MS.gene35681:intron 25.0%
!! TTGATTTGAAAGCTTACATA+AGG + chr2.1:14551779-14551798 MS.gene35681:CDS 25.0%
!! TTTCGATGAAGATGAAATTT+TGG + chr2.1:14551873-14551892 MS.gene35681:CDS 25.0%
!!! AAATGGCATTTTTCAATTTG+TGG + chr2.1:14552508-14552527 MS.gene35681:intron 25.0%
!!! AATTAAGTTTCTGGTAAGTT+AGG + chr2.1:14551961-14551980 MS.gene35681:intron 25.0%
!!! TGAATGTATTTTGGAACTTA+AGG + chr2.1:14550304-14550323 MS.gene35681:CDS 25.0%
AACCCCCATAAATGATAAAA+TGG - chr2.1:14552233-14552252 None:intergenic 30.0%
AACTGTAGATAATAATGGCA+TGG - chr2.1:14552129-14552148 None:intergenic 30.0%
AAGTATATTGCAAATGGTGT+AGG + chr2.1:14551176-14551195 MS.gene35681:intron 30.0%
ACCCCCATAAATGATAAAAT+GGG - chr2.1:14552232-14552251 None:intergenic 30.0%
AGATCAAGAAATTGCTAGTT+TGG + chr2.1:14553192-14553211 MS.gene35681:CDS 30.0%
ATCTCCTAGATTGAAATACA+TGG - chr2.1:14551361-14551380 None:intergenic 30.0%
ATGTATTTGAACTCAGGATA+AGG + chr2.1:14553165-14553184 MS.gene35681:intron 30.0%
ATTCATAACCAGTGTGAAAA+TGG - chr2.1:14553134-14553153 None:intergenic 30.0%
ATTGAAGCATGAGTAAATGA+TGG - chr2.1:14551436-14551455 None:intergenic 30.0%
CAACAAAAATCTAAGCAGAT+TGG - chr2.1:14552846-14552865 None:intergenic 30.0%
GAAGAATAGTGAAGATTCTT+TGG + chr2.1:14551801-14551820 MS.gene35681:CDS 30.0%
GAAGGAAATTAATTCAAAGG+TGG + chr2.1:14550586-14550605 MS.gene35681:CDS 30.0%
GAAGGAGTTTCAATTAAAAG+AGG + chr2.1:14550544-14550563 MS.gene35681:CDS 30.0%
GATAGAACTTAATCAAGTCA+AGG + chr2.1:14550187-14550206 MS.gene35681:CDS 30.0%
TAAAATTTGTCACCCTATAG+TGG - chr2.1:14552209-14552228 None:intergenic 30.0%
TAAATTGGATGGACAAATGA+AGG + chr2.1:14550748-14550767 MS.gene35681:CDS 30.0%
TATTATCTACAGTTAGGATG+AGG + chr2.1:14552135-14552154 MS.gene35681:intron 30.0%
TATTTGATGGAGAACTTGAT+TGG + chr2.1:14550134-14550153 MS.gene35681:CDS 30.0%
TCAATTTGTGGAGCAAAAAA+GGG + chr2.1:14552520-14552539 MS.gene35681:intron 30.0%
TCATGCTTCAATAAATACGA+GGG + chr2.1:14551444-14551463 MS.gene35681:intron 30.0%
TCTCCTAGATTGAAATACAT+GGG - chr2.1:14551360-14551379 None:intergenic 30.0%
TGATTCAAAGCTGACTAAAT+TGG + chr2.1:14550733-14550752 MS.gene35681:CDS 30.0%
TGCCATTATTATCTACAGTT+AGG + chr2.1:14552129-14552148 MS.gene35681:intron 30.0%
TGTCAATTCTCTCTAAACAA+AGG - chr2.1:14552715-14552734 None:intergenic 30.0%
TTCAATTTGTGGAGCAAAAA+AGG + chr2.1:14552519-14552538 MS.gene35681:intron 30.0%
TTGTTGAGAATCTTATCAAG+AGG + chr2.1:14552918-14552937 MS.gene35681:CDS 30.0%
TTTAGAGAGAATTGACATCT+TGG + chr2.1:14552718-14552737 MS.gene35681:intron 30.0%
! AACTGTTCAACAATGCATTT+TGG + chr2.1:14553216-14553235 MS.gene35681:CDS 30.0%
! ACCCATTTTATCATTTATGG+GGG + chr2.1:14552228-14552247 MS.gene35681:intron 30.0%
! ACTGACATGTCTAGAATAAT+AGG - chr2.1:14552427-14552446 None:intergenic 30.0%
! AGTTTTCAACCTCAACATTA+AGG + chr2.1:14551203-14551222 MS.gene35681:intron 30.0%
! GGTTTTGCAAATTAAGTTTC+TGG + chr2.1:14551952-14551971 MS.gene35681:intron 30.0%
! GTCTAGAATAATAGGTGATT+TGG - chr2.1:14552419-14552438 None:intergenic 30.0%
! GTTTTCAACCTCAACATTAA+GGG + chr2.1:14551204-14551223 MS.gene35681:intron 30.0%
! TCAGAGAAGAAGAATTTTGA+AGG + chr2.1:14550473-14550492 MS.gene35681:CDS 30.0%
! TTTTCAACCTCAACATTAAG+GGG + chr2.1:14551205-14551224 MS.gene35681:intron 30.0%
!! AAACTTAATTTGCAAAACCC+AGG - chr2.1:14551951-14551970 None:intergenic 30.0%
!! AACTTAATTTGCAAAACCCA+GGG - chr2.1:14551950-14551969 None:intergenic 30.0%
!! AATTTAGTCAGCTTTGAATC+AGG - chr2.1:14550734-14550753 None:intergenic 30.0%
!! AGAAGAAGAATTTTGAAGGA+CGG + chr2.1:14550477-14550496 MS.gene35681:CDS 30.0%
!! ATTCAAAGGTGCTGATTATT+AGG + chr2.1:14552183-14552202 MS.gene35681:intron 30.0%
!!! AATTTTGGAGCTTTTTGCAT+TGG + chr2.1:14551888-14551907 MS.gene35681:CDS 30.0%
!!! TTATGGGGGTTTTAGTTTTT+AGG + chr2.1:14552242-14552261 MS.gene35681:intron 30.0%
AAAAGCAAGGAAGGAAAACA+TGG - chr2.1:14552354-14552373 None:intergenic 35.0%
AACCAACTCCATTGAAATCA+AGG + chr2.1:14553066-14553085 MS.gene35681:CDS 35.0%
AACCTTGATTTCAATGGAGT+TGG - chr2.1:14553071-14553090 None:intergenic 35.0%
ACAAAAGCAGTAGAAATCCA+AGG - chr2.1:14551831-14551850 None:intergenic 35.0%
ACAACTAAGAGAATGTCAGT+TGG + chr2.1:14552460-14552479 MS.gene35681:intron 35.0%
ACATGCTGAAATGAAGGAAT+TGG + chr2.1:14550406-14550425 MS.gene35681:CDS 35.0%
ACTAGCAATTTCTTGATCTC+TGG - chr2.1:14553191-14553210 None:intergenic 35.0%
ATATGCAGTTGCATCTTACA+TGG + chr2.1:14551489-14551508 MS.gene35681:intron 35.0%
ATTAATGCCCCTTAATGTTG+AGG - chr2.1:14551215-14551234 None:intergenic 35.0%
ATTCAGTTGTGCATATAGCT+TGG + chr2.1:14552964-14552983 MS.gene35681:CDS 35.0%
CAAGAAACAGTTGCACTAAT+AGG - chr2.1:14551241-14551260 None:intergenic 35.0%
CAGAAGAACCTTGATTTCAA+TGG - chr2.1:14553077-14553096 None:intergenic 35.0%
CGAACAAGCAGAAAAATTAG+AGG + chr2.1:14550367-14550386 MS.gene35681:CDS 35.0%
CTCATGCTTCAATAAATACG+AGG + chr2.1:14551443-14551462 MS.gene35681:intron 35.0%
CTGATTATTAGGCCACTATA+GGG + chr2.1:14552194-14552213 MS.gene35681:intron 35.0%
CTTACACTATCCTCAACATT+CGG - chr2.1:14551739-14551758 None:intergenic 35.0%
GAATAGTGAAGATTCTTTGG+CGG + chr2.1:14551804-14551823 MS.gene35681:CDS 35.0%
GAGGTTCTTAAAAAGGAGAT+TGG + chr2.1:14553256-14553275 MS.gene35681:CDS 35.0%
GATCGATTAAGTCATACCAT+TGG + chr2.1:14551514-14551533 MS.gene35681:CDS 35.0%
GATTCTCAACAAAATCTGCA+AGG - chr2.1:14552910-14552929 None:intergenic 35.0%
GATTGATTACCTGTCAACTT+AGG - chr2.1:14550776-14550795 None:intergenic 35.0%
GGAAATTAATTCAAAGGTGG+AGG + chr2.1:14550589-14550608 MS.gene35681:CDS 35.0%
GGAGTTTCAATTAAAAGAGG+AGG + chr2.1:14550547-14550566 MS.gene35681:CDS 35.0%
TCAAAGCTGACTAAATTGGA+TGG + chr2.1:14550737-14550756 MS.gene35681:CDS 35.0%
TCAATGAAGAAGCAACTTGA+GGG + chr2.1:14550431-14550450 MS.gene35681:CDS 35.0%
TGAAGAACGTCAGAAAGAAA+TGG + chr2.1:14550658-14550677 MS.gene35681:CDS 35.0%
TTAGAGGCTAAAAAGGAGAA+TGG + chr2.1:14550383-14550402 MS.gene35681:CDS 35.0%
TTGCAAGAACACATCCAAAA+TGG + chr2.1:14553013-14553032 MS.gene35681:CDS 35.0%
! ACAGTCTGAGGTTCTTAAAA+AGG + chr2.1:14553249-14553268 MS.gene35681:CDS 35.0%
! AGTTCTGACGGTGAAAATAA+TGG - chr2.1:14552008-14552027 None:intergenic 35.0%
! CAAACATTCCATTTTCACAC+TGG + chr2.1:14553123-14553142 MS.gene35681:intron 35.0%
! GAGTAAATGATGGTGACAAT+AGG - chr2.1:14551426-14551445 None:intergenic 35.0%
! TACATATTTTCTGCCTGTTC+TGG + chr2.1:14552877-14552896 MS.gene35681:intron 35.0%
!! CACAAATCAGTTTGCCATTT+TGG - chr2.1:14553030-14553049 None:intergenic 35.0%
!! GAATTTTGAAGGACGGATAA+AGG + chr2.1:14550484-14550503 MS.gene35681:CDS 35.0%
!! GGTTCTTCTGCTTTTCTTAT+TGG + chr2.1:14553087-14553106 MS.gene35681:intron 35.0%
!! GTTTTCCTTCCTTGCTTTTA+TGG + chr2.1:14552355-14552374 MS.gene35681:intron 35.0%
AAGAAATGGTGAATGAGCTC+AGG + chr2.1:14550672-14550691 MS.gene35681:CDS 40.0%
ATCAAGAGAGAAGCGATTTG+AGG + chr2.1:14550514-14550533 MS.gene35681:CDS 40.0%
ATGAACTCAAACACTGTCTC+TGG + chr2.1:14551277-14551296 MS.gene35681:intron 40.0%
ATTAATCCACTGCGCAAGAA+GGG + chr2.1:14551649-14551668 MS.gene35681:CDS 40.0%
CCACCCATGTATTTCAATCT+AGG + chr2.1:14551354-14551373 MS.gene35681:intron 40.0%
CCTAGATTGAAATACATGGG+TGG - chr2.1:14551357-14551376 None:intergenic 40.0%
CGATTAAGTCATACCATTGG+TGG + chr2.1:14551517-14551536 MS.gene35681:CDS 40.0%
CTAAACAAAGGTGAGAACTG+TGG - chr2.1:14552703-14552722 None:intergenic 40.0%
CTCAATGAAGAAGCAACTTG+AGG + chr2.1:14550430-14550449 MS.gene35681:CDS 40.0%
CTTGAGCTACAAGCACATAA+AGG + chr2.1:14553289-14553308 MS.gene35681:CDS 40.0%
GAATGGACATGCTGAAATGA+AGG + chr2.1:14550400-14550419 MS.gene35681:CDS 40.0%
GCTGATTATTAGGCCACTAT+AGG + chr2.1:14552193-14552212 MS.gene35681:intron 40.0%
GCTTCGTCAAGTCATTGATA+AGG + chr2.1:14550232-14550251 MS.gene35681:CDS 40.0%
GGAACTCGAAGATCAAGTTA+AGG + chr2.1:14550610-14550629 MS.gene35681:CDS 40.0%
GTGATCCATAAAAGCAAGGA+AGG - chr2.1:14552363-14552382 None:intergenic 40.0%
GTTGTTCAAGCAGAAAGATG+CGG - chr2.1:14551702-14551721 None:intergenic 40.0%
TATCTACAGTTAGGATGAGG+TGG + chr2.1:14552138-14552157 MS.gene35681:intron 40.0%
TCAAGAGAGAAGCGATTTGA+GGG + chr2.1:14550515-14550534 MS.gene35681:CDS 40.0%
TCATTGATAAGGACCGTGAA+AGG + chr2.1:14550243-14550262 MS.gene35681:CDS 40.0%
TGAAGAGCAGCTCTATTTGA+TGG + chr2.1:14550121-14550140 MS.gene35681:CDS 40.0%
TGAAGTTTGTGACAAAGTGC+AGG + chr2.1:14550061-14550080 MS.gene35681:CDS 40.0%
TGACGGTGAAAATAATGGCA+TGG - chr2.1:14552003-14552022 None:intergenic 40.0%
TGCATCAAGCTCTATCTCTT+TGG - chr2.1:14550330-14550349 None:intergenic 40.0%
TGCTCAGCACAAGATAGATA+TGG + chr2.1:14551912-14551931 MS.gene35681:CDS 40.0%
TGGTGGAACAAGTTTGCAAT+TGG + chr2.1:14551534-14551553 MS.gene35681:CDS 40.0%
TGTGGTGATCCATAAAAGCA+AGG - chr2.1:14552367-14552386 None:intergenic 40.0%
! GGAATTTGAAGTCAGAGTGA+AGG + chr2.1:14550568-14550587 MS.gene35681:CDS 40.0%
! TTGCAAACTTGTTCCACCAA+TGG - chr2.1:14551533-14551552 None:intergenic 40.0%
!! TGACATCTTGGTGTCAATCT+TGG + chr2.1:14552730-14552749 MS.gene35681:intron 40.0%
!! TTCTTGCGCAGTGGATTAAT+GGG - chr2.1:14551649-14551668 None:intergenic 40.0%
AACAAAGGTGAGAACTGTGG+TGG - chr2.1:14552700-14552719 None:intergenic 45.0%
ACTGTTGCAGTTGAGTCACA+AGG + chr2.1:14551571-14551590 MS.gene35681:CDS 45.0%
ATGGAGATGTTTCAGACCCT+GGG + chr2.1:14551931-14551950 MS.gene35681:CDS 45.0%
CAGTTAGGATGAGGTGGTTA+TGG + chr2.1:14552144-14552163 MS.gene35681:intron 45.0%
CATTAATCCACTGCGCAAGA+AGG + chr2.1:14551648-14551667 MS.gene35681:CDS 45.0%
GCAGTTTGAAAGACAAGCCA+AGG + chr2.1:14550706-14550725 MS.gene35681:CDS 45.0%
GCGATTTGAGGGACAAATGA+AGG + chr2.1:14550526-14550545 MS.gene35681:CDS 45.0%
GGATTGCAAACTACAGTCTG+AGG + chr2.1:14553237-14553256 MS.gene35681:CDS 45.0%
GTTCCTCTTCCTTCCTTTCA+CGG - chr2.1:14550259-14550278 None:intergenic 45.0%
TATGGAGATGTTTCAGACCC+TGG + chr2.1:14551930-14551949 MS.gene35681:CDS 45.0%
TGATAAGGACCGTGAAAGGA+AGG + chr2.1:14550247-14550266 MS.gene35681:CDS 45.0%
TTTCACCGTCAGAACTGCTT+TGG + chr2.1:14552012-14552031 MS.gene35681:intron 45.0%
! ACAGACCAAAGCAGTTCTGA+CGG - chr2.1:14552020-14552039 None:intergenic 45.0%
! ACTATCCTCAACATTCGGTG+AGG - chr2.1:14551734-14551753 None:intergenic 45.0%
! ATGAAGGAGCCTAAGTTGAC+AGG + chr2.1:14550764-14550783 MS.gene35681:CDS 45.0%
!! CAGCTTTGAATCAGGATCCT+TGG - chr2.1:14550726-14550745 None:intergenic 45.0%
!! CTTCTTGCGCAGTGGATTAA+TGG - chr2.1:14551650-14551669 None:intergenic 45.0%
AGAGCGCAAGATAGAGCTCA+AGG + chr2.1:14550157-14550176 MS.gene35681:CDS 50.0%
ATTGACGGGGAGCAAGAATC+CGG + chr2.1:14550815-14550834 MS.gene35681:intron 50.0%
GAAGAAGCAACTTGAGGGTC+AGG + chr2.1:14550436-14550455 MS.gene35681:CDS 50.0%
GAAGATTCTTTGGCGGTCCT+TGG + chr2.1:14551811-14551830 MS.gene35681:CDS 50.0%
GAGAACCTCACCGAATGTTG+AGG + chr2.1:14551726-14551745 MS.gene35681:CDS 50.0%
GCAAGGTGCAAGACCAGAAC+AGG - chr2.1:14552893-14552912 None:intergenic 55.0%
GGACCGTGAAAGGAAGGAAG+AGG + chr2.1:14550253-14550272 MS.gene35681:CDS 55.0%
TTGTCTCCCTTCTTGCGCAG+TGG - chr2.1:14551658-14551677 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 14550043 14553318 14550043 ID=MS.gene35681
chr2.1 mRNA 14550043 14553318 14550043 ID=MS.gene35681.t1;Parent=MS.gene35681
chr2.1 exon 14550043 14550785 14550043 ID=MS.gene35681.t1.exon1;Parent=MS.gene35681.t1
chr2.1 CDS 14550043 14550785 14550043 ID=cds.MS.gene35681.t1;Parent=MS.gene35681.t1
chr2.1 exon 14551497 14551952 14551497 ID=MS.gene35681.t1.exon2;Parent=MS.gene35681.t1
chr2.1 CDS 14551497 14551952 14551497 ID=cds.MS.gene35681.t1;Parent=MS.gene35681.t1
chr2.1 exon 14552915 14553087 14552915 ID=MS.gene35681.t1.exon3;Parent=MS.gene35681.t1
chr2.1 CDS 14552915 14553087 14552915 ID=cds.MS.gene35681.t1;Parent=MS.gene35681.t1
chr2.1 exon 14553181 14553318 14553181 ID=MS.gene35681.t1.exon4;Parent=MS.gene35681.t1
chr2.1 CDS 14553181 14553318 14553181 ID=cds.MS.gene35681.t1;Parent=MS.gene35681.t1
Gene Sequence

>MS.gene35681

TGTTGTTAGAAGAATTGATGAAGTTTGTGACAAAGTGCAGGAAAAAACTGAAGAATGTGTTAAGGATTTTGTAGTGAATGAAGAGCAGCTCTATTTGATGGAGAACTTGATTGGAGAGCGCAAGATAGAGCTCAAGGCAAAAGAGATAGAACTTAATCAAGTCAAGGGTAACATTTCAAAAGAAATAGAGCTTCGTCAAGTCATTGATAAGGACCGTGAAAGGAAGGAAGAGGAACTCAAAGCTCTTTCCCAAAAAATTGATGAATGTATTTTGGAACTTAAGGCCAAAGAGATAGAGCTTGATGCAATGAACAAATTAATTGGCGAACAAGCAGAAAAATTAGAGGCTAAAAAGGAGAATGGACATGCTGAAATGAAGGAATTGGACTCAATGAAGAAGCAACTTGAGGGTCAGGTTAATAAACTTGAGTCAGAGAAGAAGAATTTTGAAGGACGGATAAAGGATTTAGAATCAAGAGAGAAGCGATTTGAGGGACAAATGAAGGAGTTTCAATTAAAAGAGGAGGAATTTGAAGTCAGAGTGAAGGAAATTAATTCAAAGGTGGAGGAACTCGAAGATCAAGTTAAGGAGCTCGAATCAGAGAAGAAGAAATTTGAAGAACGTCAGAAAGAAATGGTGAATGAGCTCAGGTTAAATGAGAAGCAGTTTGAAAGACAAGCCAAGGATCCTGATTCAAAGCTGACTAAATTGGATGGACAAATGAAGGAGCCTAAGTTGACAGGTAATCAATCTGAAGCATTAAGAAAATATATTGACGGGGAGCAAGAATCCGGTAAATGTTCTTATTCATATAGTTTACTGAAAGAATTTCATTTCAGTTGCTTCTTTTAATGATGTTGATATTTTTAATAACTTGATATACATAATTCTTTAAAATTATTTTTAATCGTATTAATGTTGTACGCTTAGTCGATTTGAAAAGCTTCTTCAGTTTTCATATCACACACACGCACACACTGTCAAGCTGTATTCGAATTTCACCCTTGAACATTTTATGAATCTCTCAAGTGCAAAAAACTCTTGCATTTTTTTTATTCATGGCTAAGGCTTAATTATAATATTATGGTACTGCTAATGAAAGAGTAGAATAGTAATTTAGTTTTAATTTTTCAAGTATATTGCAAATGGTGTAGGTTCAAGTTTTCAACCTCAACATTAAGGGGCATTAATAGTCCTATTAGTGCAACTGTTTCTTGTCCTGTTTATTAATCAATGAACTCAAACACTGTCTCTGGTGTTGTGTCGACATTGATTGTAACAGTTAATAATTAATATAATGCTTTCTTTCGCCACCCATGTATTTCAATCTAGGAGATGTCTTACATTCATGCATAGTATTTTTTATTTTTTTTTTAATTCCTATTGTCACCATCATTTACTCATGCTTCAATAAATACGAGGGAGCTTAAATATCTGCTATCTAAATATGCAGTTGCATCTTACATGGATGATCGATTAAGTCATACCATTGGTGGAACAAGTTTGCAATTGGACACAAGTGAGAAAACTGTTGCAGTTGAGTCACAAGGCAATAACATTCTAGTTAGTCGAGCAAAAGTTGTTTTAGATATAATACAGAAACCCATTAATCCACTGCGCAAGAAGGGAGACAATGCTGTGATTTTTGATGATAGCCGCATCTTTCTGCTTGAACAACTGATGAGAACCTCACCGAATGTTGAGGATAGTGTAAGAGAAGCGCTGACGCTAGCACTTGATTTGAAAGCTTACATAAGGAAGAATAGTGAAGATTCTTTGGCGGTCCTTGGATTTCTACTGCTTTTGTCAATTTATCGATTGCTTACTTCTTTCGATGAAGATGAAATTTTGGAGCTTTTTGCATTGGTTGCTCAGCACAAGATAGATATGGAGATGTTTCAGACCCTGGGTTTTGCAAATTAAGTTTCTGGTAAGTTAGGATGACTATGTTACTTTCCATGCCATTATTTTCACCGTCAGAACTGCTTTGGTCTGTGATCACTATCTCCTTTTATCTTCATGTTTTGATATCTTATATTGTATCATATGATTATAAAATTTGTTAATATCTATTGTTACTTTCCATGCCATTATTATCTACAGTTAGGATGAGGTGGTTATGGCTATTCATGATCAAAGATTCAAAGGTGCTGATTATTAGGCCACTATAGGGTGACAAATTTTACCCATTTTATCATTTATGGGGGTTTTAGTTTTTAGGAATTTGTTATTGCAATTCAAATTATATTGATATGACAGAAAAAGTCAATAATATGATAGTTGCATGAGTCGTGAAATATAAATATGCCATGTTTTCCTTCCTTGCTTTTATGGATCACCACATTTATTCATATCAAAACAAGTAGTACAAACCAAATCACCTATTATTCTAGACATGTCAGTAAAAAACAAAATAACAACTAAGAGAATGTCAGTTGGCATTAGAGTAGTTTTTCTTTTGCTAAAATGGCATTTTTCAATTTGTGGAGCAAAAAAGGGATAAATTAAAGATAATGGCTGAAATGAATCCTTTTAAGAAAATAGGACAACTCTTGTTAAAATCTGTTAAAGTCAGATGATTTTAAATACAGAGATTATGATATTAGTTGTTGGTACGAAATTGAACTGATATGTAATTGGATTAATTTTGTTTCCACCACAGTTCTCACCTTTGTTTAGAGAGAATTGACATCTTGGTGTCAATCTTGGAAAATGAAATAAAGTTCATATTTATATCTCTCTCTATTTATGCATCTCTTCTATTTTCTAAATGGTTTGACAATTTTTGAAATGTTCAGTCCAATCTGCTTAGATTTTTGTTGTTGTTAATTGGTACATATTTTCTGCCTGTTCTGGTCTTGCACCTTGCAGATTTTGTTGAGAATCTTATCAAGAGGAAGCAATTTGTTGTAGCTGTTAGATTCAGTTGTGCATATAGCTTGGCTGACAAGACTAAACAAGTTGATCTCTTGCAAGAACACATCCAAAATGGCAAACTGATTTGTGAGAGCAGATGCAAGAAAACCAACTCCATTGAAATCAAGGTTCTTCTGCTTTTCTTATTGGATTTCTATTCTATCAAACATTCCATTTTCACACTGGTTATGAATAAATTATTACTATGTATTTGAACTCAGGATAAGGCCAGAGATCAAGAAATTGCTAGTTTGGAAACTGTTCAACAATGCATTTTGGATTGCAAACTACAGTCTGAGGTTCTTAAAAAGGAGATTGGATATCGCATTCTTGAGCTACAAGCACATAAAGGAAAGTAG
Protein sequence

>MS.gene35681.t1

CCUKNUUSLUQSAGKNURMCUGFCSEURAALFDGELDWRAQDRAQGKRDRTUSSQGUHFKRNRASSSHUUGPUKEGRGTQSSFPKNUUMYFGTUGQRDRAUCNEQINWRTSRKIRGUKGEWTCUNEGIGLNEEATUGSGUUTUVREEEFURTDKGFRIKREAIUGTNEGVSIKRGGIUSQSEGNUFKGGGTRRSSUGARIREEEIURTSERNGEUAQVKUEAVUKTSQGSUFKADUIGWTNEGAUVDSCILHGUSIKSYHWWNKFAIGHKUENCCSUVTRQUHSSUSSKSCFRYNTETHUSTAQEGRQCCDFUUUPHLSAUTTDENLTECUGUCKRSADASTUFESLHKEEUURFFGGPWISTAFVNLSIAYFFRURUNFGAFCIGCSAQDRYGDVSDPGFCUESYQEEAICCSCUIQLCIULGUQDUTSUSLARTHPKWQTDLUEQMQENQLHUNQGUGQRSRNCUFGNCSTMHFGLQTTVUGSUKGDWISHSUATSTURKV