AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35852

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 256 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGGTCCAGCTTAAAATTT+TGG 0.143087 4.1:-58253677 None:intergenic
TGTTTCAATGCCTTCAAATT+TGG 0.164452 4.1:-58254786 None:intergenic
TACGACATGGATCAACTTTC+TGG 0.182893 4.1:+58255098 MS.gene35852:CDS
CGTTGCTATGGGTAAATTAA+TGG 0.191276 4.1:+58253408 MS.gene35852:CDS
GACGCTATCTGCTTCGTTCT+TGG 0.221041 4.1:+58251942 MS.gene35852:CDS
TGGAACAGCATACCTATATT+TGG 0.222219 4.1:+58255010 MS.gene35852:CDS
CGTCAAAGCTCGCAATTTCT+TGG 0.225100 4.1:+58252208 MS.gene35852:CDS
TGCTTTAAAGACTGACTTAA+AGG 0.229765 4.1:+58254326 MS.gene35852:CDS
GGAAGATTCTTCAAATTTCT+TGG 0.242859 4.1:-58253806 None:intergenic
GGTACTTCTGCCACTGGTTC+TGG 0.251193 4.1:-58257959 None:intergenic
TCGCAATTTCTTGGTCTTCC+AGG 0.267745 4.1:+58252217 MS.gene35852:CDS
CGGAGAAGGGACCGATGATT+TGG 0.271085 4.1:-58251869 None:intergenic
CTATTTCAGACACTTTAATA+TGG 0.278501 4.1:-58253888 None:intergenic
GTCCATTAGGTTTGATTTAC+CGG 0.279199 4.1:-58251922 None:intergenic
CTTCAACCAACCTAGGGTTT+TGG 0.290857 4.1:+58251766 None:intergenic
TAGTGAAGCTCAAACTAATT+TGG 0.293996 4.1:+58253027 MS.gene35852:CDS
CTTAATCTGATGATTAAGTT+TGG 0.301894 4.1:-58254514 None:intergenic
TTTGATTTACCGGCGCCATT+AGG 0.302446 4.1:-58251912 None:intergenic
ATTGATGTAGTTGGTGCATT+AGG 0.312719 4.1:-58258555 None:intergenic
TCTCTTAACTTGATCAGTTC+TGG 0.312964 4.1:-58254039 None:intergenic
TATCTATGTTACCAGATATA+CGG 0.314142 4.1:-58254944 None:intergenic
GGAACGTATTGAGAAGTTAA+TGG 0.320984 4.1:+58254545 MS.gene35852:CDS
ACCCGATGTTATCAAGTTAA+AGG 0.322615 4.1:+58252715 MS.gene35852:CDS
TCGAAAGATTACGCTCATTA+GGG 0.329739 4.1:+58253548 MS.gene35852:CDS
GTAGAATAAAAGAGGAAGTT+AGG 0.335771 4.1:+58252945 MS.gene35852:CDS
TCAGTAGGACACTAAGAAAT+AGG 0.336660 4.1:+58254682 MS.gene35852:CDS
TTGATTTACCGGCGCCATTA+GGG 0.349523 4.1:-58251911 None:intergenic
AAAAGTAAAGTAAGTTCCTT+TGG 0.351545 4.1:-58252269 None:intergenic
ACTTGATTCTTCCTCAATCT+TGG 0.353601 4.1:-58258604 None:intergenic
GAACGTATTGAGAAGTTAAT+GGG 0.354299 4.1:+58254546 MS.gene35852:CDS
GGTGCTTTCAACCGTATATC+TGG 0.355800 4.1:+58254933 MS.gene35852:CDS
ATCGAAAGATTACGCTCATT+AGG 0.359793 4.1:+58253547 MS.gene35852:CDS
AAGTTTGGATAAGTTTGTTT+TGG 0.366147 4.1:-58254499 None:intergenic
AATCTGGCTTACGCAATATA+AGG 0.372578 4.1:-58258045 None:intergenic
GGATTGTGAAAAGGAAATTC+AGG 0.375716 4.1:+58254452 MS.gene35852:CDS
AGTTGGAGTATGAGCAAAAT+CGG 0.375779 4.1:+58254262 MS.gene35852:CDS
CGAAGCAGATAGCGTCCATT+AGG 0.378707 4.1:-58251935 None:intergenic
CTACTGACAGCTACAGTATC+TGG 0.379627 4.1:-58257869 None:intergenic
GGAGAGATATAATTTGTTCA+TGG 0.384149 4.1:+58254911 MS.gene35852:CDS
GTTGTTCTGTTTCTTTCTGA+AGG 0.384495 4.1:-58257937 None:intergenic
CTCCGCTGCTAACTTTGCTT+CGG 0.390561 4.1:-58254367 None:intergenic
AGATTATACTTCTTCTGTGT+TGG 0.399676 4.1:-58253376 None:intergenic
CGCAATTTCTTGGTCTTCCA+GGG 0.400472 4.1:+58252218 MS.gene35852:CDS
GAAAGAGCTGTGTGTGTTGC+AGG 0.401857 4.1:+58253165 MS.gene35852:CDS
AATGAATGACGTACAGGAAA+AGG 0.403782 4.1:+58252868 MS.gene35852:CDS
GAAGATACACCAAAGGATAA+TGG 0.406123 4.1:+58258651 MS.gene35852:CDS
GGGCAATTCTCTTTGCTGTT+GGG 0.408231 4.1:+58253623 MS.gene35852:CDS
TGAGAAATGCCATCTATTCC+TGG 0.409219 4.1:-58258262 None:intergenic
TCCTTTAACTTGATAACATC+GGG 0.411307 4.1:-58252716 None:intergenic
CAGACAACAACTTCCTCAAA+AGG 0.412884 4.1:+58258283 MS.gene35852:CDS
CGTTGAGGTTCTCCATTCAT+AGG 0.413105 4.1:+58255147 MS.gene35852:CDS
ACAGCCATCATCGGCCCTAA+TGG 0.413636 4.1:+58251897 MS.gene35852:CDS
TTCAAAATTAAAATATCAGT+TGG 0.414114 4.1:+58254245 MS.gene35852:CDS
ACCGTAGAGGAATAAATGTC+TGG 0.417311 4.1:-58253504 None:intergenic
TTCAACATTGAGCGATGAAA+AGG 0.419132 4.1:+58253984 MS.gene35852:CDS
TCTATTCCTGGAATGTTACT+TGG 0.421091 4.1:-58258250 None:intergenic
GGAATTCTGCTGACACAATC+TGG 0.421978 4.1:+58253727 MS.gene35852:CDS
GCTTTAAAGACTGACTTAAA+GGG 0.422881 4.1:+58254327 MS.gene35852:CDS
GAAATAGGAGGCACTGTGAC+AGG 0.423935 4.1:+58254697 MS.gene35852:CDS
AGGAAAAGGGTCAAAATGTT+GGG 0.425639 4.1:+58252882 MS.gene35852:CDS
GGAGAAGGGACCGATGATTT+GGG 0.427285 4.1:-58251868 None:intergenic
CGCCGGTAAATCAAACCTAA+TGG 0.427347 4.1:+58251920 MS.gene35852:CDS
ACAAAGCTGACGTGTTAGTC+GGG 0.427441 4.1:+58255597 MS.gene35852:CDS
AAGACTTCTGCAAATCAGTT+GGG 0.428911 4.1:+58254136 MS.gene35852:CDS
AGAAAAGTATGAATCAAAGT+TGG 0.429088 4.1:+58253837 MS.gene35852:CDS
AGTAACATTCCAGGAATAGA+TGG 0.434295 4.1:+58258253 MS.gene35852:CDS
GAGTTCTCGAATTCAAGAAT+TGG 0.437343 4.1:+58254290 MS.gene35852:CDS
AGGGCAATTCTCTTTGCTGT+TGG 0.438745 4.1:+58253622 MS.gene35852:CDS
GCCGATGATGGCTGTGAAGC+CGG 0.439827 4.1:-58251889 None:intergenic
ATGGATACCGATAGCTCAAC+AGG 0.441028 4.1:+58254636 MS.gene35852:CDS
GGTGAAAGTGGTCTTGACTT+TGG 0.444210 4.1:-58258690 None:intergenic
CAAGAGAGAGTACGAGAAGT+TGG 0.444711 4.1:+58252319 MS.gene35852:CDS
TCCAATCATATCAGATCCTA+TGG 0.445708 4.1:+58254617 MS.gene35852:CDS
TATTAAAGTGTCTGAAATAG+AGG 0.448340 4.1:+58253891 MS.gene35852:CDS
GATTCAGTTTGATCCTTCCT+TGG 0.452042 4.1:+58253597 MS.gene35852:CDS
TGAACTAGGAGGCTTTAAAT+CGG 0.454611 4.1:-58258526 None:intergenic
ACGTTCTGCCTTGGTTCGCT+TGG 0.455871 4.1:+58252049 MS.gene35852:CDS
ACTCTAAAACGCTTGTTTCA+AGG 0.456559 4.1:+58253322 MS.gene35852:CDS
AGAATCTGTCCATTATCCTT+TGG 0.458097 4.1:-58258660 None:intergenic
GATGCTCCAAGTAACATTCC+AGG 0.459808 4.1:+58258244 MS.gene35852:CDS
TCGACGGTAATGTGGTTACC+TGG 0.464557 4.1:+58252147 MS.gene35852:CDS
TTGAATGCAGCTCTGACTCC+CGG 0.464721 4.1:-58253075 None:intergenic
TACTTGATGCCATGTTGAAA+TGG 0.476994 4.1:-58255047 None:intergenic
AGAATATAGTAGAATAAAAG+AGG 0.477378 4.1:+58252937 MS.gene35852:CDS
CAAATCATCGGTCCCTTCTC+CGG 0.477655 4.1:+58251870 MS.gene35852:CDS
TCCATAGGATCTGATATGAT+TGG 0.478350 4.1:-58254618 None:intergenic
TCTAGCCGTTACCGTTGCTA+TGG 0.480885 4.1:+58253396 MS.gene35852:CDS
AAAAGAGATGAATCAGTTGA+AGG 0.483918 4.1:+58254398 MS.gene35852:CDS
CTGAAAACCCTTTCCATCAT+CGG 0.484387 4.1:-58255540 None:intergenic
CGCTTGTTTCAAGGAGTCCA+TGG 0.490083 4.1:+58253331 MS.gene35852:CDS
CCCAGACATTTATTCCTCTA+CGG 0.490701 4.1:+58253503 MS.gene35852:CDS
TTGTTCTGTTTCTTTCTGAA+GGG 0.491002 4.1:-58257936 None:intergenic
GCAAGAACCATCACTGCAGC+AGG 0.492129 4.1:+58252107 MS.gene35852:CDS
TAGATTCAATGCGGTTAAGC+AGG 0.492151 4.1:+58254890 MS.gene35852:CDS
CTGTGAAGGTGTTGGTAAAG+AGG 0.492508 4.1:+58252571 MS.gene35852:CDS
CAAACAACTCTACCTATGAA+TGG 0.492953 4.1:-58255159 None:intergenic
ACACAATCTGGCACAATGAC+TGG 0.495148 4.1:+58253739 MS.gene35852:CDS
AACTGGACCTGTTGAGCTAT+CGG 0.495167 4.1:-58254643 None:intergenic
CTGTTTCTCGCTGTCATCGA+AGG 0.496893 4.1:-58252018 None:intergenic
TGAACATGAAGCCAATGAAA+AGG 0.497806 4.1:+58252604 MS.gene35852:CDS
CAAGTCAGAGGATTGTGAAA+AGG 0.498418 4.1:+58254443 MS.gene35852:CDS
CAGGAAAAGGGTCAAAATGT+TGG 0.498813 4.1:+58252881 MS.gene35852:CDS
TCAACATTGAGCGATGAAAA+GGG 0.502116 4.1:+58253985 MS.gene35852:CDS
GAAGAATGAACGTTCTGCCT+TGG 0.502748 4.1:+58252040 MS.gene35852:CDS
GCATTAGGAGATGGTGAACT+AGG 0.505609 4.1:-58258540 None:intergenic
ACAGCATCAAACCATGTCAC+TGG 0.506557 4.1:+58251797 MS.gene35852:CDS
AAAGCGGCTTACGCTGCATA+AGG 0.506601 4.1:-58258322 None:intergenic
TTGAGAAGTTAATGGGGAAA+AGG 0.506689 4.1:+58254553 MS.gene35852:CDS
TTGAGAACCACGGAGGTGAA+TGG 0.508057 4.1:-58251973 None:intergenic
TCCGGCTTCACAGCCATCAT+CGG 0.509679 4.1:+58251888 MS.gene35852:CDS
GAGAGATATAATTTGTTCAT+GGG 0.510530 4.1:+58254912 MS.gene35852:CDS
GTGGTCTTGACTTTGGCTCA+TGG 0.511247 4.1:-58258683 None:intergenic
CCTCCGTGGTTCTCAACTAA+AGG 0.512065 4.1:+58251980 MS.gene35852:CDS
AAAGACTTCTGCAAATCAGT+TGG 0.513705 4.1:+58254135 MS.gene35852:CDS
TCTTCCACAGAAAGTTGAGC+TGG 0.515131 4.1:-58258633 None:intergenic
CAAGATACCGATGATGGAAA+GGG 0.515187 4.1:+58255533 MS.gene35852:CDS
TGAAGCCAATGAAAAGGAAA+AGG 0.517556 4.1:+58252610 MS.gene35852:CDS
CTCCTTTAACTTGATAACAT+CGG 0.518360 4.1:-58252717 None:intergenic
AGGAACAGCACCAAATTTGA+AGG 0.519324 4.1:+58254776 MS.gene35852:CDS
TTTATCTGTTTCTTGCCACT+AGG 0.521179 4.1:-58258187 None:intergenic
GGATCGAGATGGCAATGACT+TGG 0.521535 4.1:+58252913 MS.gene35852:CDS
GAACTAGGAGGCTTTAAATC+GGG 0.522104 4.1:-58258525 None:intergenic
TCTTGTTTGTGAAGATCTTG+AGG 0.522838 4.1:+58253651 MS.gene35852:CDS
GAAGTTAATGGGGAAAAGGC+AGG 0.523484 4.1:+58254557 MS.gene35852:CDS
CAGCATCAAACCATGTCACT+GGG 0.524678 4.1:+58251798 MS.gene35852:CDS
GTTTGATCCTTCCTTGGAGA+GGG 0.525060 4.1:+58253603 MS.gene35852:CDS
GTGACATTAGCCGAGGGAGC+AGG 0.525239 4.1:-58257893 None:intergenic
TCAAGATACCGATGATGGAA+AGG 0.526229 4.1:+58255532 MS.gene35852:CDS
CATGTCGTAGAATGCCTTTG+TGG 0.527501 4.1:-58255084 None:intergenic
TCTTTCTGAAGAGTAATGCT+AGG 0.527702 4.1:-58257980 None:intergenic
ATGTCGTAGAATGCCTTTGT+GGG 0.527952 4.1:-58255083 None:intergenic
ACTTGCGCCTGCTGCAGTGA+TGG 0.528810 4.1:-58252114 None:intergenic
TGATGAAGTTGATGCTGCAT+TGG 0.528838 4.1:+58255442 MS.gene35852:CDS
CTTGGAGCATCTGAAGAACT+AGG 0.529227 4.1:-58258232 None:intergenic
GAAATCAACTAGGGTGAAAG+TGG 0.531442 4.1:-58258702 None:intergenic
ATGAATGACGTACAGGAAAA+GGG 0.531554 4.1:+58252869 MS.gene35852:CDS
ATACTGCGAAGGAATGCATC+AGG 0.533222 4.1:+58253455 MS.gene35852:CDS
GGCGCCATTAGGGCCGATGA+TGG 0.536952 4.1:-58251901 None:intergenic
GCTAATCAAGATACCGATGA+TGG 0.537866 4.1:+58255527 MS.gene35852:CDS
AAAGAGATGAATCAGTTGAA+GGG 0.537967 4.1:+58254399 MS.gene35852:CDS
TCTTTCTGAAGGGAAATGCT+AGG 0.538109 4.1:-58257926 None:intergenic
TCAAAATGTTGGGGTCGAAA+AGG 0.539260 4.1:+58252892 MS.gene35852:CDS
CAATTGCTGCGCTTGCGTTG+AGG 0.539925 4.1:+58255132 MS.gene35852:CDS
GGCGAAAATGAATGACGTAC+AGG 0.542374 4.1:+58252862 MS.gene35852:CDS
ATTGAAACAGTATGATTCCC+TGG 0.543584 4.1:+58254800 MS.gene35852:CDS
AACAAAGCTGACGTGTTAGT+CGG 0.543657 4.1:+58255596 MS.gene35852:CDS
AGACAACAACTTCCTCAAAA+GGG 0.544213 4.1:+58258284 MS.gene35852:CDS
CGAAAGATTACGCTCATTAG+GGG 0.545759 4.1:+58253549 MS.gene35852:CDS
ATTCGTTCAAAATCTTGTGA+AGG 0.547590 4.1:+58255497 MS.gene35852:CDS
CTGTGACAGGGATAAAATAG+AGG 0.547709 4.1:+58254710 MS.gene35852:CDS
GATGGCTGTGAAGCCGGAGA+AGG 0.547816 4.1:-58251883 None:intergenic
TAATCATCAGATTAAGTCAA+AGG 0.548082 4.1:+58254521 MS.gene35852:CDS
CCGTAGAGGAATAAATGTCT+GGG 0.548920 4.1:-58253503 None:intergenic
TGCTAAGTTTGCTGATACTG+TGG 0.548991 4.1:+58253297 MS.gene35852:CDS
TTGACTTTGATGAGCTCAGT+AGG 0.554607 4.1:+58254667 MS.gene35852:CDS
TGAAGGGTGACTATGAAAAG+TGG 0.556060 4.1:+58254415 MS.gene35852:CDS
CAAAATCTTGTGAAGGAGCG+AGG 0.556645 4.1:+58255504 MS.gene35852:CDS
AATACAAGAAAAGTGTGAGC+TGG 0.556858 4.1:+58254581 MS.gene35852:CDS
TGCAAAGAATAAGGATGACC+TGG 0.557363 4.1:+58253132 MS.gene35852:CDS
CTAGCCGTTACCGTTGCTAT+GGG 0.557768 4.1:+58253397 MS.gene35852:CDS
GTTGGAGTATGAGCAAAATC+GGG 0.561779 4.1:+58254263 MS.gene35852:CDS
TTTAGCATTGTAGACATCCC+AGG 0.564950 4.1:-58252165 None:intergenic
CACAAAGGCATTCTACGACA+TGG 0.567383 4.1:+58255085 MS.gene35852:CDS
AGCTGTCATGAAAGAAGAGA+AGG 0.567878 4.1:+58254857 MS.gene35852:CDS
CACTTCTTCTGTGAGTGAGG+TGG 0.568641 4.1:+58258581 MS.gene35852:CDS
AATGGATGCAATTGTTGTTG+AGG 0.570500 4.1:+58253426 MS.gene35852:CDS
CAGAATTTCAAGTCATACAA+AGG 0.572120 4.1:+58251843 MS.gene35852:CDS
TTTCGAGCAGATATCTGGCT+CGG 0.573865 4.1:+58252289 MS.gene35852:CDS
ATGGCTGTGAAGCCGGAGAA+GGG 0.574175 4.1:-58251882 None:intergenic
GGAGTCAGAGCTGCATTCAA+AGG 0.577242 4.1:+58253078 MS.gene35852:CDS
CTTTAAAGACTGACTTAAAG+GGG 0.578368 4.1:+58254328 MS.gene35852:CDS
TTAAAAGTTGTAACTGTGGA+CGG 0.578698 4.1:+58253706 MS.gene35852:CDS
ATGTCTGGGGAGGAATCCGC+AGG 0.581973 4.1:-58253489 None:intergenic
GGCTTCTCAAAATGCAGCAG+AGG 0.582336 4.1:+58254197 MS.gene35852:CDS
ATCATCGGCCCTAATGGCGC+CGG 0.582495 4.1:+58251903 MS.gene35852:CDS
TGTCAGTAGTCCTGCTCCCT+CGG 0.582785 4.1:+58257883 MS.gene35852:CDS
ATGCTAGGTACTTCTGCCAC+TGG 0.582920 4.1:-58257965 None:intergenic
AGAATTGCCCTCTCCAAGGA+AGG 0.585346 4.1:-58253610 None:intergenic
TGGTGGCACTAGTGGTGGAA+TGG 0.586320 4.1:+58253759 MS.gene35852:CDS
TGGCCCAGCTCAACTTTCTG+TGG 0.586457 4.1:+58258629 MS.gene35852:CDS
ATTGAGGAAGAATCAAGTAA+TGG 0.587628 4.1:+58258609 MS.gene35852:CDS
TACAAAGGTTCCCAAATCAT+CGG 0.587890 4.1:+58251858 MS.gene35852:CDS
CACCTCACTCACAGAAGAAG+TGG 0.588449 4.1:-58258580 None:intergenic
GCATCTGAAGAACTAGGCGT+TGG 0.588737 4.1:-58258226 None:intergenic
CGACGGTAATGTGGTTACCT+GGG 0.589141 4.1:+58252148 MS.gene35852:CDS
GAATCAAAGTTGGAAGAGCT+AGG 0.590268 4.1:+58253847 MS.gene35852:CDS
GAAGTGGATATTGATGTAGT+TGG 0.593034 4.1:-58258564 None:intergenic
AAATTCAGGAACGGAAGAAG+AGG 0.595234 4.1:+58254466 MS.gene35852:CDS
GTTGATAAAACAAGCTGTGA+AGG 0.597464 4.1:+58252557 MS.gene35852:CDS
TGCTTCTGTGACATTAGCCG+AGG 0.600583 4.1:-58257900 None:intergenic
CATTAATTTACCCATAGCAA+CGG 0.600751 4.1:-58253407 None:intergenic
GTATGAAGAAAATCAACTCA+AGG 0.602074 4.1:+58254176 MS.gene35852:CDS
TTTACCCATAGCAACGGTAA+CGG 0.602629 4.1:-58253401 None:intergenic
GTTGAGCTATCGGTATCCAT+AGG 0.605904 4.1:-58254633 None:intergenic
GAGTGAGGTGGCCAAGATTG+AGG 0.606108 4.1:+58258593 MS.gene35852:CDS
CAACATTGAGCGATGAAAAG+GGG 0.606910 4.1:+58253986 MS.gene35852:CDS
ATCCACTTCTTCTGTGAGTG+AGG 0.607118 4.1:+58258578 MS.gene35852:CDS
AACAGAACAACCAGAACCAG+TGG 0.608935 4.1:+58257949 MS.gene35852:CDS
AACAACTTCCTCAAAAGGGA+AGG 0.609863 4.1:+58258288 MS.gene35852:CDS
CTTCCACAGAAAGTTGAGCT+GGG 0.610376 4.1:-58258632 None:intergenic
GGTAGAATACAAGCAAAACA+TGG 0.610506 4.1:+58254731 MS.gene35852:CDS
AAAGAGAATTGCCCTCTCCA+AGG 0.611260 4.1:-58253614 None:intergenic
TAGGATCTGATATGATTGGA+AGG 0.611282 4.1:-58254614 None:intergenic
ACAATGACTGGTGGCACTAG+TGG 0.612466 4.1:+58253751 MS.gene35852:CDS
AGTTTGATCCTTCCTTGGAG+AGG 0.612781 4.1:+58253602 MS.gene35852:CDS
CCTTCTTCAGTTAAGCCTAG+TGG 0.613481 4.1:+58258172 MS.gene35852:intron
TGTTTGTGAAGATCTTGAGG+AGG 0.613588 4.1:+58253654 MS.gene35852:CDS
TTGATAAACCAAGCGAACCA+AGG 0.614939 4.1:-58252057 None:intergenic
TGAAATTCTGTATCTCGAGA+CGG 0.616444 4.1:-58251830 None:intergenic
GCATTGATGTCTAAGATCGA+AGG 0.617141 4.1:+58254756 MS.gene35852:CDS
AGACTTCTGCAAATCAGTTG+GGG 0.617793 4.1:+58254137 MS.gene35852:CDS
GTACTTCATCGACGGTAATG+TGG 0.619581 4.1:+58252139 MS.gene35852:CDS
GACCGAAGCAAAGTTAGCAG+CGG 0.619751 4.1:+58254365 MS.gene35852:CDS
AAATAGGAGGCACTGTGACA+GGG 0.620082 4.1:+58254698 MS.gene35852:CDS
TGAGGATGAAAATACTGCGA+AGG 0.620388 4.1:+58253444 MS.gene35852:CDS
TCGTAGAATGCCTTTGTGGG+AGG 0.621429 4.1:-58255080 None:intergenic
GCTTCTGTGACATTAGCCGA+GGG 0.622291 4.1:-58257899 None:intergenic
AAAACAAGCTGTGAAGGTGT+TGG 0.624308 4.1:+58252563 MS.gene35852:CDS
TATGCTGAGATTGAAAAGCA+AGG 0.625200 4.1:+58253946 MS.gene35852:CDS
AAAGATCAATCATGCGACCA+TGG 0.625528 4.1:-58253348 None:intergenic
GTGAAAAGGAAATTCAGGAA+CGG 0.632020 4.1:+58254457 MS.gene35852:CDS
GACATGGTTTGATGCTGTAG+TGG 0.632473 4.1:-58251792 None:intergenic
AATTCAGGAACGGAAGAAGA+GGG 0.632676 4.1:+58254467 MS.gene35852:CDS
CTAGTGGTGGAATGGAAGCA+AGG 0.633127 4.1:+58253767 MS.gene35852:CDS
AAAATCTTGTGAAGGAGCGA+GGG 0.637426 4.1:+58255505 MS.gene35852:CDS
ATCAACTTCAAGTTCCTCAG+TGG 0.637706 4.1:-58252540 None:intergenic
GTAGGACACTAAGAAATAGG+AGG 0.639401 4.1:+58254685 MS.gene35852:CDS
CGATGTTATCAAGTTAAAGG+AGG 0.639471 4.1:+58252718 MS.gene35852:CDS
ATGACTGGTGGCACTAGTGG+TGG 0.639638 4.1:+58253754 MS.gene35852:CDS
CAAAGCTGACGTGTTAGTCG+GGG 0.639845 4.1:+58255598 MS.gene35852:CDS
GACATGGATCAACTTTCTGG+TGG 0.644379 4.1:+58255101 MS.gene35852:CDS
GAGATTAAAAGTTGTAACTG+TGG 0.646711 4.1:+58253702 MS.gene35852:CDS
CACTGCTATGCCTCCCACAA+AGG 0.649518 4.1:+58255070 MS.gene35852:CDS
TTCTGTGGAAGATACACCAA+AGG 0.650284 4.1:+58258644 MS.gene35852:CDS
AAAGTGGAAATCCAAGTCAG+AGG 0.651200 4.1:+58254431 MS.gene35852:CDS
TTAGGAGATGGTGAACTAGG+AGG 0.652667 4.1:-58258537 None:intergenic
TCATCAGATTAAGTCAAAGG+AGG 0.653398 4.1:+58254524 MS.gene35852:CDS
TACGCAATATAAGGAGAAAG+CGG 0.655412 4.1:-58258036 None:intergenic
GTTCCTTTAGTTGAGAACCA+CGG 0.655844 4.1:-58251983 None:intergenic
GGGGTCGAAAAGGATCGAGA+TGG 0.658796 4.1:+58252902 MS.gene35852:CDS
CAATGGATTCAACATCACCC+TGG 0.659318 4.1:-58252235 None:intergenic
AACGTATTGAGAAGTTAATG+GGG 0.659365 4.1:+58254547 MS.gene35852:CDS
CAATCTGGCACAATGACTGG+TGG 0.659717 4.1:+58253742 MS.gene35852:CDS
GGAAAAGGGTCAAAATGTTG+GGG 0.661319 4.1:+58252883 MS.gene35852:CDS
GTTAGAACCATTCACCTCCG+TGG 0.671957 4.1:+58251966 MS.gene35852:CDS
GCAAGTCAGTACTTCATCGA+CGG 0.672701 4.1:+58252131 MS.gene35852:CDS
AGAGGAATAAATGTCTGGGG+AGG 0.674962 4.1:-58253499 None:intergenic
GTAGTTGGTGCATTAGGAGA+TGG 0.677079 4.1:-58258549 None:intergenic
TCAGGCATTTAAAAGACCTG+CGG 0.678777 4.1:+58253473 MS.gene35852:CDS
GAAAGATTACGCTCATTAGG+GGG 0.684912 4.1:+58253550 MS.gene35852:CDS
CGTAGAGGAATAAATGTCTG+GGG 0.688856 4.1:-58253502 None:intergenic
AACAGAACAACTGAAACCAG+TGG 0.695910 4.1:+58258003 MS.gene35852:CDS
GAAGACGATCCATTTCAACA+TGG 0.722638 4.1:+58255038 MS.gene35852:CDS
CCTTTAGTTGAGAACCACGG+AGG 0.731694 4.1:-58251980 None:intergenic
AGCATCAAACCATGTCACTG+GGG 0.736134 4.1:+58251799 MS.gene35852:CDS
TGCACACGAATAGACCGTAG+AGG 0.737569 4.1:-58253517 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.1 gene 58251781 58258716 58251781 ID=MS.gene35852
chr4.1 mRNA 58251781 58258716 58251781 ID=MS.gene35852.t1;Parent=MS.gene35852
chr4.1 exon 58251781 58252379 58251781 ID=MS.gene35852.t1.exon1;Parent=MS.gene35852.t1
chr4.1 CDS 58251781 58252379 58251781 ID=cds.MS.gene35852.t1;Parent=MS.gene35852.t1
chr4.1 exon 58252509 58255168 58252509 ID=MS.gene35852.t1.exon2;Parent=MS.gene35852.t1
chr4.1 CDS 58252509 58255168 58252509 ID=cds.MS.gene35852.t1;Parent=MS.gene35852.t1
chr4.1 exon 58255415 58255619 58255415 ID=MS.gene35852.t1.exon3;Parent=MS.gene35852.t1
chr4.1 CDS 58255415 58255619 58255415 ID=cds.MS.gene35852.t1;Parent=MS.gene35852.t1
chr4.1 exon 58257860 58258055 58257860 ID=MS.gene35852.t1.exon4;Parent=MS.gene35852.t1
chr4.1 CDS 58257860 58258055 58257860 ID=cds.MS.gene35852.t1;Parent=MS.gene35852.t1
chr4.1 exon 58258182 58258332 58258182 ID=MS.gene35852.t1.exon5;Parent=MS.gene35852.t1
chr4.1 CDS 58258182 58258332 58258182 ID=cds.MS.gene35852.t1;Parent=MS.gene35852.t1
chr4.1 exon 58258521 58258716 58258521 ID=MS.gene35852.t1.exon6;Parent=MS.gene35852.t1
chr4.1 CDS 58258521 58258716 58258521 ID=cds.MS.gene35852.t1;Parent=MS.gene35852.t1
Gene Sequence

>MS.gene35852

GGTTTTGGTTTCCACTACAGCATCAAACCATGTCACTGGGGAAAATTCACCGTCTCGAGATACAGAATTTCAAGTCATACAAAGGTTCCCAAATCATCGGTCCCTTCTCCGGCTTCACAGCCATCATCGGCCCTAATGGCGCCGGTAAATCAAACCTAATGGACGCTATCTGCTTCGTTCTTGGCGTTAGAACCATTCACCTCCGTGGTTCTCAACTAAAGGAACTTATCTATGCTGCCTTCGATGACAGCGAGAAACAGAAGAATGAACGTTCTGCCTTGGTTCGCTTGGTTTATCAACTCGCAGACAAATCTGAGATTCAGTTTGCAAGAACCATCACTGCAGCAGGCGCAAGTCAGTACTTCATCGACGGTAATGTGGTTACCTGGGATGTCTACAATGCTAAACTCGTATCTCTTGACATTCTCGTCAAAGCTCGCAATTTCTTGGTCTTCCAGGGTGATGTTGAATCCATTGCATCGAAAAATCCAAAGGAACTTACTTTACTTTTCGAGCAGATATCTGGCTCGGATCAATTCAAGAGAGAGTACGAGAAGTTGGAAGAAGAAAAAAATTCTGCTGAGGAGAAATTGAAACTTGTTTATCAGAAGAAAAAGACTATTTTGATGGAGAAAAAGAAGAAGAAAGAACAAAAGAAAAAGGCAGAGGAACATCTTCGGTTGCAAGATGAATTGAAATCTATGAAAAAGGAACATTTCTTGTGGAAGTTGTTCAATATAGAGAATGATTTTGCTAAAACCACTGAGGAACTTGAAGTTGATAAAACAAGCTGTGAAGGTGTTGGTAAAGAGGTAGAAAAATTTGAACATGAAGCCAATGAAAAGGAAAAGGAGCTAACCAGATGTGTTGAAAAGATTAAGCTACTCGAGAAGAAAATAACTGAGAAAAGCAACAAACTTGATAACAAGACTCGACCCGATGTTATCAAGTTAAAGGAGGAAATATCTCGTATCAATTTGAAAATTGAAAAAACGAAGAAGGAGCTCAGCAAAAAAGGTGAAGAACGAAAGAGGCATGCTAATGATATAGCAATGCTAAAGAGAAGTTTTCAGGATCTTCAGGCGAAAATGAATGACGTACAGGAAAAGGGTCAAAATGTTGGGGTCGAAAAGGATCGAGATGGCAATGACTTGGAAGAATATAGTAGAATAAAAGAGGAAGTTAGGATAAAAACTGCAAATCTTAGAGAGAAAAAAGAATTTCTTGATGGGCAGCAACGTGCTGATAGTGAAGCTCAAACTAATTTGGAAGAAACTCTTCAACAATTAAAAAACCGGGAGTCAGAGCTGCATTCAAAGGAAAAACAGATGCGAGAAAGGCTTGATAATTTTGCAAAGAATAAGGATGACCTGGAAAACGCGAAGAAAGAGCTGTGTGTGTTGCAGGAAAAACAAAGAGATTTCAAGCGAGAATATGATGTCTTGAAGAAAAAAATTGGTGATGTAGTAAATGAACTACATGAATTGAAATCTGATAGATATGAAAACGAGAGAGATGCTAAGTTTGCTGATACTGTGGCGACTCTAAAACGCTTGTTTCAAGGAGTCCATGGTCGCATGATTGATCTTTGTAGACCAACACAGAAGAAGTATAATCTAGCCGTTACCGTTGCTATGGGTAAATTAATGGATGCAATTGTTGTTGAGGATGAAAATACTGCGAAGGAATGCATCAGGCATTTAAAAGACCTGCGGATTCCTCCCCAGACATTTATTCCTCTACGGTCTATTCGTGTGCAGCTAATAATCGAAAGATTACGCTCATTAGGGGGTACCACAAAATTGGTTTTTGATGTGATTCAGTTTGATCCTTCCTTGGAGAGGGCAATTCTCTTTGCTGTTGGGAACACTCTTGTTTGTGAAGATCTTGAGGAGGCCAAAATTTTAAGCTGGACCGGTGAGAGATTAAAAGTTGTAACTGTGGACGGAATTCTGCTGACACAATCTGGCACAATGACTGGTGGCACTAGTGGTGGAATGGAAGCAAGGTCAAAAAAGTGGGATGCCAAGAAATTTGAAGAATCTTCCCAGAAGAAAGAAAAGTATGAATCAAAGTTGGAAGAGCTAGGATCAACTAGAGATATGCACCATATTAAAGTGTCTGAAATAGAGGAGAAAATTAGCGAACTCAAGAAGAAAGCTCAATATGCTGAGATTGAAAAGCAAGGCATTGAAAAGAAGCTTTCAACATTGAGCGATGAAAAGGGGACTATCAAAAAAGAAATTGAACGAGTTAGTCCAGAACTGATCAAGTTAAGAGATTCTGTTGAAAAGAGAAATGCAGAAATACGCGAGCTTGAGAAGAGTATAAATGAAATCACTGATGAGATATACAAAGACTTCTGCAAATCAGTTGGGGTTGCAAACATTCGCGAGTATGAAGAAAATCAACTCAAGGCTTCTCAAAATGCAGCAGAGGAGAGACTGAACCTGAGTAGCCAACTTTCAAAATTAAAATATCAGTTGGAGTATGAGCAAAATCGGGACATGAGTTCTCGAATTCAAGAATTGGAATCTTCGCTGAGTGCTTTAAAGACTGACTTAAAGGGGCTACAGAACAAAGAGACCGAAGCAAAGTTAGCAGCGGAGAATGCTACAAAAGAGATGAATCAGTTGAAGGGTGACTATGAAAAGTGGAAATCCAAGTCAGAGGATTGTGAAAAGGAAATTCAGGAACGGAAGAAGAGGGCTTCTGCAGCCAAAACAAACTTATCCAAACTTAATCATCAGATTAAGTCAAAGGAGGAACGTATTGAGAAGTTAATGGGGAAAAGGCAGGAAATACAAGAAAAGTGTGAGCTGGAGCAGATTAACCTTCCAATCATATCAGATCCTATGGATACCGATAGCTCAACAGGTCCAGTTTTTGACTTTGATGAGCTCAGTAGGACACTAAGAAATAGGAGGCACTGTGACAGGGATAAAATAGAGGTAGAATACAAGCAAAACATGGATGCATTGATGTCTAAGATCGAAGGAACAGCACCAAATTTGAAGGCATTGAAACAGTATGATTCCCTGGTTGAAAAAGAAAGACTTATAGCTGAAGAGTTTGAAGCTGTCATGAAAGAAGAGAAGGAAAAAACAGATAGATTCAATGCGGTTAAGCAGGAGAGATATAATTTGTTCATGGGTGCTTTCAACCGTATATCTGGTAACATAGATAAAATCTACAAACAACTTACAAAGAGCAACACACATCTTTTTGGTGGAACAGCATACCTATATTTGGAAAATGAAGACGATCCATTTCAACATGGCATCAAGTACACTGCTATGCCTCCCACAAAGGCATTCTACGACATGGATCAACTTTCTGGTGGCGAAAAGACAATTGCTGCGCTTGCGTTGAGGTTCTCCATTCATAGGTAGAGTTGTTTGTACTTATCTTTCTATATGAGCATTTTTGGTTTGAGAAATTATTTTTTTTTACTTTTAATTATAAAACTATCCTTATTGTTTTTACTTTATTTCCTATTTATAAATATTTGCGTATGTAAACAAAAAAAATGTTATTTAAATTTTGAAGAGTTCAACCAAGTAATAAATATACTTAAACATCTTGCCTATAAAGAATGTCTACTAACATGGTTCCCGCCTCCTTATCTTTTCAGTTATAAGCCTTCACCATTTTTCATACTTGATGAAGTTGATGCTGCATTGGACAACTTGAATGTTGCGAAAGTTGCTAGCTTTATTCGTTCAAAATCTTGTGAAGGAGCGAGGGCTAATCAAGATACCGATGATGGAAAGGGTTTTCAGATAATTCAAATATCACAGAAAGATCACTTCTATAACAAAGCTGACGTGTTAGTCGGGGTTTGCAGAGACTCTAAAAGTGGCAGTTCGAGAACTCTCTTGCTTGATCTCACTAAATACCGGGAGTCCTAAATTTCATTCAATCAATACATTTTGTTAGTTAAGTTCTTTGTAATGTATGGAGCTCAATTTTCTATGTATAGAAATCTTCACTTGGGTTCTTCCTTTCTAATGGGAGTTCATACTACTTCATCTATATCTATACTTTATTTTACTCTCATTTATCTTTCAATGTCTGAGTGGTTTACTTGGTATTTCTAACCATTTTCTAAACATTAAAATAGTTCTTAAATTGTATCTGTTTTGGACCTTAGTATCTGTATTATCTAATTCATATTCAATTAATTTCTTTTCTATTTGTTGTCACTGATTGATTTCTTAATCTGTTACTTTTTTCCTTAAAAATAGATTTTGCCTCTATAGATGTACATAGATTAGTTACCAGTGTGGTGAAATGGCACTCATGGTGCCACAATGACGGAATCACATGGTGGATTTTCGGACAACCATCATAGGCAATTTGTAAAGGAACGCCAACCATTGTGGCGCCATAGGTCGCAATGGCGTGTTTATATGGCGGATGTTTGGCTTTCTCCTATATTCCGCCATCCACTGGAGACAACATCGTTAGTTACAAAATAACCTGAAAGTTAAAGTGTTAGTCTGGATTTACGAGGACTCTAAAAGAGGCTGTTTGAGAACTCTCATTTTATCTCACTAAGAGATCTGGATAGGTATATGTTAGCAAGTCCTACTGTCAAATATATGTGGAGCAATATAATGTGGCTTTGAAAGAATTATTTAGTTGTAGTTTGGGTGTTGCAATATAATGTGGAATTGGGTTAAGCTACTGTGTGATGTAACTTTCATCTCACAACTTTGAAGTTTGGCTCACTAAGAATTATTCTTGTGGTAATGAGCTTTATATCGGGAGTTCTAACCTTCACCCATTTGATACGTTATATAAGTTAAGTTGCTTGCGATGTACAGAGCTCGATTTGCAATTTTACGATAATAATTATCAAAAAGTGTCATTATTCTACACCACTTTTATATTTGTCGGTTCAACTGATAGGTAGAAATTTCTTTTTATAGAAAATGTTAGTATGTTAATTATTATCGAACCTTGAACCTATAACTTAAATTGTTATCTGTTTTGGACCTCAGTATCTGGATTATCTAATTCGTATTCGGTATAATTTCTTTACTATTTGCTGTCACTGATTGATTTGTGAATCTGATACTTTTTTCCTCCAAAATAGATTTTGTCTCTTATATGTACAGAGATTAGTTATCATTGTTGTTAAATGGCACTCATGGCGCCACCATGGTGGAATCACATGGCAGATTTTTGGACAGCCATCATAGAAAATTTGTGAAGGAACGCCCGTCATTGTGGCGCCATACGTCGCAAAGGCGTGTTTATATGACGGATGTTTGGCTTTCTCCTATATCCCGCCATCCACTGGCGACAACATCGTTTGTATGTGGATTTTATCTTTGGTGCTCACTAAGAATTATTCTGTGCTAGTGAGCTTTATATCGGGCGTCCTAACCTTCATCTATTCGATACGTTAGATAAATTAAATTGCTTGCGATGTACAGAGCTCGATTTGCAATTTTACAATAATAATTATCAAAAAGTGTCATTATTCTACACGACTTTTTTTTTTTTTTTTAAGAAATTCTACACGACTTTTATATTTGTTGGTTTCAACTAACAGGTAGGTTTTTTATTTTTTTATATGTAAAATGTTAATTGTTCTCTTTTTTTATGGTTTTTGATTCAATTCTAGAGCCCTTTTTCCTGTAATTTGGGTGCTGTTAAGGTGTAGCATTGGTGTTCAGAAGCTGTTGTTGGCTTGGTGTTTTTCATTTTTTACAACATCAGACTGTGGACTCTTGTATCAAGTTACATTTTGTGATCTGTATCTTGAACTTAATGAAAATTTTGTTAGGTTTAGTCTTGAGAAAGAAGGATTCTTGATCATACAATTCAACTCCTCTAGCCTAGGGCAAATACAAATAAATAAATAAATGGATGGATCATCCCCTTTATTTGAAAGGGAGGGCTTTCGTACCAAACCTGTGGCTGAATGGTTAAAGCATTCCTTTTTTTGACCGCAGCACCATATCTTGACCCTTTTCTCGAATTGGTCCATTTCGAGTTGCCCACTCCCCTTCGACTGGTTCTCATAGTTCAACATTCCTGTTAATTGATCTACGGTTTTCTTTGGAAGGTACAGAAGCCAGATACTGTAGCTGTCAGTAGTCCTGCTCCCTCGGCTAATGTCACAGAAGCAGTACCTAGCATTTCCCTTCAGAAAGAAACAGAACAACCAGAACCAGTGGCAGAAGTACCTAGCATTACTCTTCAGAAAGAAACAGAACAACTGAAACCAGTGGCAAAAAACAGCCGCTTTCTCCTTATATTGCGTAAGCCAGATTGCTAATATTTGTTGACAGCAAATTTTTATGTGGACTATGGAGGCCAAATAACTTATTTGGTATATACATTTTCAGTTATGATGATTTAAAACCACCGTCATCCCCCTTCTTCAGTTAAGCCTAGTGGCAAGAAACAGATAAATATCAGTGATGCAGTGCCAACGCCTAGTTCTTCAGATGCTCCAAGTAACATTCCAGGAATAGATGGCATTTCTCAGACAACAACTTCCTCAAAAGGGAAGGAGTCTCTTTCTCCTTATGCAGCGTAAGCCGCTTTCTAATTTTGTGGTGATATCGTACGTTAGTACACATTTTCTATCATCTTTCTCATCTAAACTTCTTTGATTCGGCGGCTATGAGGATTTGAAACCTCCCAGTTCACTCTTGTTGTCAACTACTTTAGCACTTTTATTTGTGGTATCTTGTGGTTTGTTTTCCTTTATATACCTGCAGATATCCCGATTTAAAGCCTCCTAGTTCACCATCTCCTAATGCACCAACTACATCAATATCCACTTCTTCTGTGAGTGAGGTGGCCAAGATTGAGGAAGAATCAAGTAATGGCCCAGCTCAACTTTCTGTGGAAGATACACCAAAGGATAATGGACAGATTCTCCATGAGCCAAAGTCAAGACCACTTTCACCCTAG
Protein sequence

>MS.gene35852.t1

GFGFHYSIKPCHWGKFTVSRYRISSHTKVPKSSVPSPASQPSSALMAPVNQTUWTLSASFLALEPFTSVVLNURNLSMLPSMTARNRRMNVLPWFAWFINSQTNLRFSLQEPSLQQAQVSTSSTVMWLPGMSTMLNSYLLTFSSKLAISWSSRVMLNPLHRKIQRNLLYFSSRYLARINSRESTRSWKKKKILLRRNUNFCSIURMILLKPLRNLKLIKQAVKVLVKRUKNLNMKPMKRKRSUPDVLKRLSYSRRKULRKATNLITRLDPMLSSURRKYLVSIUKLKKRRRSSAKKVKNERGMLMIUQCUREVFRIFRRKUMTYRKRVKMLGSKRIEMAMTWKNIVEUKRKLGUKLQILERKKNFLMGSNVLIVKLKLIWKKLFNNUKTGSQSCIQRKNRCEKGLIILQRIRMTWKTRRKSCVCCRKNKEISSENMMSURKKLVMUUMNYMNUNLIDMKTREMLSLLILWRLUNACFKESMVAULIFVDQHRRSIIUPLPLLWVNUWMQLLLRMKILRRNASGIUKTCGFLPRHLFLYGLFVCSUUSKDYAHUGVPQNWFLMUFSLILPWRGQFSLLLGTLLFVKILRRPKFUAGPVRDUKLULWTEFCUHNLAQULVALVVEWKQGQKSGMPRNLKNLPRRKKSMNQSWKSUDQLEICTILKCLKURRKLANSRRKLNMLRLKSKALKRSFQHUAMKRGLSKKKLNELVQNUSSUEILLKREMQKYASLRRVUMKSLMRYTKTSANQLGLQTFASMKKINSRLLKMQQRRDUTUVANFQNUNISWSMSKIGTUVLEFKNWNLRUVLURLTURGYRTKRPKQSUQRRMLQKRUISURVTMKSGNPSQRIVKRKFRNGRRGLLQPKQTYPNLIIRLSQRRNVLRSUWGKGRKYKKSVSWSRLTFQSYQILWIPIAQQVQFLTLMSSVGHUEIGGTVTGIKURUNTSKTWMHUCLRSKEQHQIURHUNSMIPWLKKKDLULKSLKLSUKKRRKKQIDSMRLSRRDIICSWVLSTVYLVTUIKSTNNLQRATHIFLVEQHTYIWKMKTIHFNMASSTLLCLPQRHSTTWINFLVAKRQLLRLRUGSPFIVISLHHFSYLMKLMLHWTTUMLRKLLALFVQNLVKERGLIKIPMMERVFRUFKYHRKITSITKLTCUSGYRSQILULSVVLLPRLMSQKQYLAFPFRKKQNNQNQWQKYLALLFRKKQNNUNQWQKTAAFSLYCLSLVARNRUISVMQCQRLVLQMLQVTFQEUMAFLRQQLPQKGRSLFLLMQHIPIUSLLVHHLLMHQLHQYPLLLUVRWPRLRKNQVMAQLNFLWKIHQRIMDRFSMSQSQDHFHP