AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35857


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35857.t1 MTR_2g088720 96.212 132 5 0 1 132 1 132 2.65e-89 255
MS.gene35857.t1 MTR_2g088720 96.212 132 5 0 1 132 12 143 3.32e-89 255
MS.gene35857.t1 MTR_7g106600 83.077 130 22 0 4 133 1 130 1.43e-76 223
MS.gene35857.t1 MTR_7g106580 78.947 133 28 0 1 133 1 133 1.97e-73 215
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35857.t1 AT5G62980 63.492 126 45 1 5 129 4 129 5.87e-52 160
MS.gene35857.t1 AT5G62980 63.492 126 45 1 5 129 4 129 5.87e-52 160
MS.gene35857.t1 AT3G11750 62.810 121 44 1 10 129 21 141 9.45e-51 158
MS.gene35857.t1 AT3G21730 52.500 120 56 1 11 129 40 159 5.10e-41 134
MS.gene35857.t1 AT3G21730 46.032 63 33 1 68 129 13 75 1.50e-13 62.0

Find 0 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATAGCTAAGAAAGTTATTGA+AGG + 15132:43953-43972 MS.gene35857:CDS 25.0%
TAAAATTGCAATCGCTACTT+TGG + 15132:44009-44028 MS.gene35857:CDS 30.0%
! AAGTAGCGATTGCAATTTTA+TGG - 15132:44009-44028 None:intergenic 30.0%
! AGTAGCGATTGCAATTTTAT+GGG - 15132:44008-44027 None:intergenic 30.0%
! TTGACTTTCTATGGTTTTCA+CGG + 15132:43803-43822 MS.gene35857:CDS 30.0%
!! AAGGTGTTGATGCATTAATA+GGG + 15132:43756-43775 MS.gene35857:CDS 30.0%
GGAGACAAACTTATTCTAAG+GGG + 15132:43779-43798 MS.gene35857:CDS 35.0%
GGGAGACAAACTTATTCTAA+GGG + 15132:43778-43797 MS.gene35857:CDS 35.0%
! AAGTTATCAGATTTTCCAGC+TGG - 15132:43899-43918 None:intergenic 35.0%
!! AGGTGTTGATGCATTAATAG+GGG + 15132:43757-43776 MS.gene35857:CDS 35.0%
!! GAAGGTGTTGATGCATTAAT+AGG + 15132:43755-43774 MS.gene35857:CDS 35.0%
GGGGAGACAAACTTATTCTA+AGG + 15132:43777-43796 MS.gene35857:CDS 40.0%
TCAACGCCTAAGTAATCAAC+CGG - 15132:44100-44119 None:intergenic 40.0%
! CTAAGGGGATTGACTTTCTA+TGG + 15132:43794-43813 MS.gene35857:CDS 40.0%
!! GGTGTTGATGCATTAATAGG+GGG + 15132:43758-43777 MS.gene35857:CDS 40.0%
AAAGCCTCATGTGGCAATTC+AGG + 15132:44072-44091 MS.gene35857:CDS 45.0%
AAGCCTCATGTGGCAATTCA+GGG + 15132:44073-44092 MS.gene35857:CDS 45.0%
ACAGAAGTTTGTGGTAGACG+TGG + 15132:43853-43872 MS.gene35857:CDS 45.0%
GATATCTGCTGTTCGTGTGA+AGG + 15132:44045-44064 MS.gene35857:CDS 45.0%
! TGGTTTTCACGGAGCAATAG+CGG + 15132:43814-43833 MS.gene35857:CDS 45.0%
!! TCAAAACCTTCTGGAGTCAG+TGG + 15132:43982-44001 MS.gene35857:CDS 45.0%
AGGACCAGCTCAAAACCTTC+TGG + 15132:43973-43992 MS.gene35857:CDS 50.0%
CAGGGTCCGGTTGATTACTT+AGG + 15132:44091-44110 MS.gene35857:CDS 50.0%
GAAGGTTGGAAAGCCTCATG+TGG + 15132:44063-44082 MS.gene35857:CDS 50.0%
GACGCTAGGACAGAAGTTTG+TGG + 15132:43844-43863 MS.gene35857:CDS 50.0%
TCATGTGGCAATTCAGGGTC+CGG + 15132:44078-44097 MS.gene35857:CDS 50.0%
TCTGCTGTTCGTGTGAAGGT+TGG + 15132:44049-44068 MS.gene35857:CDS 50.0%
TGGTTGGATCTCAAACCAGC+TGG + 15132:43881-43900 MS.gene35857:CDS 50.0%
TTGTGGTAGACGTGGATGCT+TGG + 15132:43861-43880 MS.gene35857:CDS 50.0%
! ACGGAGCAATAGCGGAAGAA+AGG + 15132:43822-43841 MS.gene35857:CDS 50.0%
! ATAGCGGAAGAAAGGACGCT+AGG + 15132:43830-43849 MS.gene35857:CDS 50.0%
!! GTTGAGATTCTGAGACGCAG+AGG + 15132:44115-44134 MS.gene35857:CDS 50.0%
!!! GACTCCAGAAGGTTTTGAGC+TGG - 15132:43980-43999 None:intergenic 50.0%
GGACCCTGAATTGCCACATG+AGG - 15132:44079-44098 None:intergenic 55.0%
GGTAGACGTGGATGCTTGGT+TGG + 15132:43865-43884 MS.gene35857:CDS 55.0%
!! TATGGGCCACTGACTCCAGA+AGG - 15132:43991-44010 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
15132 gene 43752 44153 43752 ID=MS.gene35857
15132 mRNA 43752 44153 43752 ID=MS.gene35857.t1;Parent=MS.gene35857
15132 exon 43752 44153 43752 ID=MS.gene35857.t1.exon1;Parent=MS.gene35857.t1
15132 CDS 43752 44153 43752 ID=cds.MS.gene35857.t1;Parent=MS.gene35857.t1
Gene Sequence

>MS.gene35857

ATGGAAGGTGTTGATGCATTAATAGGGGGAGACAAACTTATTCTAAGGGGATTGACTTTCTATGGTTTTCACGGAGCAATAGCGGAAGAAAGGACGCTAGGACAGAAGTTTGTGGTAGACGTGGATGCTTGGTTGGATCTCAAACCAGCTGGAAAATCTGATAACTTGTCTGATTCTTTTAGTTACGCTGAAATATATCTTATAGCTAAGAAAGTTATTGAAGGACCAGCTCAAAACCTTCTGGAGTCAGTGGCCCATAAAATTGCAATCGCTACTTTGGAAGTTCAGAAAAAGATATCTGCTGTTCGTGTGAAGGTTGGAAAGCCTCATGTGGCAATTCAGGGTCCGGTTGATTACTTAGGCGTTGAGATTCTGAGACGCAGAGGCGATTTGACAGACTAG

Protein sequence

>MS.gene35857.t1

MEGVDALIGGDKLILRGLTFYGFHGAIAEERTLGQKFVVDVDAWLDLKPAGKSDNLSDSFSYAEIYLIAKKVIEGPAQNLLESVAHKIAIATLEVQKKISAVRVKVGKPHVAIQGPVDYLGVEILRRRGDLTD