Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35933.t1 | XP_003597016.2 | 97.6 | 85 | 2 | 0 | 1 | 85 | 65 | 149 | 1.20E-35 | 158.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35933.t1 | Q8VZU2 | 64.3 | 84 | 30 | 0 | 2 | 85 | 61 | 144 | 2.9e-22 | 105.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35933.t1 | A0A072VC55 | 97.6 | 85 | 2 | 0 | 1 | 85 | 65 | 149 | 8.7e-36 | 158.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051198 | MS.gene35933 | 0.81128 | 7.58E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene35933 | 0.809302 | 2.02E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene35933 | 0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene35933 | 0.825682 | 4.10E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35933.t1 | MTR_2g088700 | 97.647 | 85 | 2 | 0 | 1 | 85 | 65 | 149 | 4.67e-54 | 169 |
| MS.gene35933.t1 | MTR_2g088700 | 97.647 | 85 | 2 | 0 | 1 | 85 | 65 | 149 | 4.85e-54 | 169 |
| MS.gene35933.t1 | MTR_6g028140 | 57.647 | 85 | 36 | 0 | 1 | 85 | 60 | 144 | 3.60e-28 | 103 |
| MS.gene35933.t1 | MTR_4g090900 | 44.706 | 85 | 47 | 0 | 1 | 85 | 60 | 144 | 5.64e-19 | 79.0 |
| MS.gene35933.t1 | MTR_2g012270 | 44.706 | 85 | 47 | 0 | 1 | 85 | 59 | 143 | 1.52e-18 | 77.8 |
| MS.gene35933.t1 | MTR_5g012010 | 40.698 | 86 | 50 | 1 | 1 | 85 | 77 | 162 | 2.36e-13 | 63.5 |
| MS.gene35933.t1 | MTR_5g020670 | 37.349 | 83 | 51 | 1 | 4 | 85 | 70 | 152 | 3.38e-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35933.t1 | AT3G03800 | 59.524 | 84 | 34 | 0 | 2 | 85 | 62 | 145 | 6.10e-30 | 107 |
| MS.gene35933.t1 | AT3G03800 | 59.524 | 84 | 34 | 0 | 2 | 85 | 62 | 145 | 6.10e-30 | 107 |
| MS.gene35933.t1 | AT5G08080 | 64.286 | 84 | 30 | 0 | 2 | 85 | 61 | 144 | 2.65e-28 | 101 |
| MS.gene35933.t1 | AT5G08080 | 64.286 | 84 | 30 | 0 | 2 | 85 | 61 | 144 | 2.65e-28 | 101 |
| MS.gene35933.t1 | AT5G08080 | 64.286 | 84 | 30 | 0 | 2 | 85 | 61 | 144 | 8.03e-28 | 102 |
| MS.gene35933.t1 | AT5G08080 | 64.286 | 84 | 30 | 0 | 2 | 85 | 61 | 144 | 8.03e-28 | 102 |
| MS.gene35933.t1 | AT5G08080 | 64.286 | 84 | 30 | 0 | 2 | 85 | 72 | 155 | 9.70e-28 | 102 |
| MS.gene35933.t1 | AT1G11250 | 37.647 | 85 | 53 | 0 | 1 | 85 | 54 | 138 | 2.45e-13 | 63.5 |
| MS.gene35933.t1 | AT1G61290 | 36.471 | 85 | 54 | 0 | 1 | 85 | 59 | 143 | 7.75e-13 | 62.0 |
| MS.gene35933.t1 | AT1G61290 | 36.471 | 85 | 54 | 0 | 1 | 85 | 59 | 143 | 7.75e-13 | 62.0 |
| MS.gene35933.t1 | AT4G03330 | 36.471 | 85 | 54 | 0 | 1 | 85 | 62 | 146 | 1.08e-12 | 61.6 |
| MS.gene35933.t1 | AT3G11820 | 37.805 | 82 | 51 | 0 | 3 | 84 | 41 | 122 | 3.45e-12 | 60.5 |
| MS.gene35933.t1 | AT3G11820 | 37.805 | 82 | 51 | 0 | 3 | 84 | 72 | 153 | 3.58e-12 | 60.5 |
| MS.gene35933.t1 | AT3G52400 | 37.349 | 83 | 52 | 0 | 2 | 84 | 70 | 152 | 3.82e-11 | 57.4 |
Find 0 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTATATAGATTTCAAATA+CGG | - | 15128:11774-11793 | MS.gene35933:intron | 15.0% |
| ! | ACGGATAAAAAATAGTTAAC+CGG | - | 15128:11793-11812 | MS.gene35933:intron | 25.0% |
| AAATAGATGTGATGTCAGAA+TGG | + | 15128:11571-11590 | MS.gene35932:intergenic | 30.0% | |
| ATTCTGCATAAAACATATCC+CGG | + | 15128:11815-11834 | MS.gene35933:intergenic | 30.0% | |
| CATTGTAACAATACCTTTCA+TGG | + | 15128:11389-11408 | MS.gene35933:intergenic | 30.0% | |
| CGGATAAAAAATAGTTAACC+GGG | - | 15128:11794-11813 | MS.gene35933:intron | 30.0% | |
| GAAAAGGATATTGATGAAGT+TGG | - | 15128:11659-11678 | MS.gene35933:CDS | 30.0% | |
| TATCAAAAAGAGGATGGAAA+AGG | - | 15128:11643-11662 | MS.gene35933:intron | 30.0% | |
| !!! | TCATAAGATTTTGCTGTTAC+TGG | - | 15128:11456-11475 | MS.gene35933:intron | 30.0% |
| AAGTTGGAAAGATTGCACAT+GGG | - | 15128:11675-11694 | MS.gene35933:CDS | 35.0% | |
| ATAGCTGAGAATGATGTAAG+AGG | + | 15128:11626-11645 | MS.gene35933:intergenic | 35.0% | |
| ATGTGATGTCAGAATGGAAA+AGG | + | 15128:11565-11584 | MS.gene35933:intergenic | 35.0% | |
| CATTCTCAGCTATCAAAAAG+AGG | - | 15128:11633-11652 | MS.gene35933:intron | 35.0% | |
| CTAAACAATAGACAAAAGCC+TGG | - | 15128:11835-11854 | MS.gene35933:intron | 35.0% | |
| TTGCAGTTCCTTGACTAAAA+AGG | - | 15128:11980-11999 | MS.gene35933:CDS | 35.0% | |
| !! | ATCTCTGACCTTTTTAGTCA+AGG | + | 15128:11991-12010 | MS.gene35933:intergenic | 35.0% |
| AGTTGGAAAGATTGCACATG+GGG | - | 15128:11676-11695 | MS.gene35933:CDS | 40.0% | |
| CATTCTTGCTCTGTCAATAC+CGG | + | 15128:11875-11894 | MS.gene35933:intergenic | 40.0% | |
| CTCAGCTATCAAAAAGAGGA+TGG | - | 15128:11637-11656 | MS.gene35933:intron | 40.0% | |
| GAAGTTGGAAAGATTGCACA+TGG | - | 15128:11674-11693 | MS.gene35933:CDS | 40.0% | |
| GTCAAGGAACTGCAATTTCA+AGG | + | 15128:11975-11994 | MS.gene35933:intergenic | 40.0% | |
| ! | CTGTTACTGGTTACTGAATC+AGG | - | 15128:11469-11488 | MS.gene35933:CDS | 40.0% |
| !! | ACAAAAGCATCTGCCATGAA+AGG | - | 15128:11373-11392 | MS.gene35933:intron | 40.0% |
| AGATCTCATGACAGAGTTCC+AGG | - | 15128:12007-12026 | MS.gene35933:CDS | 45.0% | |
| ACAAAAGCCTGGCTGTGAGA+AGG | - | 15128:11846-11865 | MS.gene35933:intron | 50.0% | |
| CAAAAGCCTGGCTGTGAGAA+GGG | - | 15128:11847-11866 | MS.gene35933:intron | 50.0% | |
| ! | CCTGGCTGTGAGAAGGGCAC+CGG | - | 15128:11853-11872 | MS.gene35933:intron | 65.0% |
| CCGGTGCCCTTCTCACAGCC+AGG | + | 15128:11856-11875 | MS.gene35933:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15128 | gene | 11346 | 12028 | 11346 | ID=MS.gene35933 |
| 15128 | mRNA | 11346 | 12028 | 11346 | ID=MS.gene35933.t1;Parent=MS.gene35933 |
| 15128 | exon | 11980 | 12028 | 11980 | ID=MS.gene35933.t1.exon1;Parent=MS.gene35933.t1 |
| 15128 | CDS | 11980 | 12028 | 11980 | ID=cds.MS.gene35933.t1;Parent=MS.gene35933.t1 |
| 15128 | exon | 11644 | 11732 | 11644 | ID=MS.gene35933.t1.exon2;Parent=MS.gene35933.t1 |
| 15128 | CDS | 11644 | 11732 | 11644 | ID=cds.MS.gene35933.t1;Parent=MS.gene35933.t1 |
| 15128 | exon | 11469 | 11542 | 11469 | ID=MS.gene35933.t1.exon3;Parent=MS.gene35933.t1 |
| 15128 | CDS | 11469 | 11542 | 11469 | ID=cds.MS.gene35933.t1;Parent=MS.gene35933.t1 |
| 15128 | exon | 11346 | 11388 | 11346 | ID=MS.gene35933.t1.exon4;Parent=MS.gene35933.t1 |
| 15128 | CDS | 11346 | 11388 | 11346 | ID=cds.MS.gene35933.t1;Parent=MS.gene35933.t1 |
| Gene Sequence |
| Protein sequence |
