AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35937

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35937.t1 MTR_2g088400 98.496 399 6 0 1 399 109 507 0.0 787
MS.gene35937.t1 MTR_2g088400 98.567 349 5 0 1 349 109 457 0.0 680
MS.gene35937.t1 MTR_4g048000 82.206 399 71 0 1 399 121 519 0.0 673
MS.gene35937.t1 MTR_2g022980 71.867 391 110 0 1 391 105 495 0.0 589
MS.gene35937.t1 MTR_8g069470 51.654 393 189 1 1 393 107 498 2.37e-143 417
MS.gene35937.t1 MTR_1g021740 53.728 389 179 1 1 389 92 479 4.33e-141 411
MS.gene35937.t1 MTR_5g033100 52.030 394 188 1 1 394 113 505 2.03e-136 400
MS.gene35937.t1 MTR_5g032720 48.492 398 203 2 1 397 93 489 5.32e-130 383
MS.gene35937.t1 MTR_7g087430 47.716 394 203 2 3 396 1 391 1.41e-126 371
MS.gene35937.t1 MTR_5g090290 51.880 399 191 1 1 398 103 501 7.33e-126 375
MS.gene35937.t1 MTR_7g087400 48.586 389 198 1 1 389 87 473 5.26e-123 365
MS.gene35937.t1 MTR_7g087410 47.826 391 203 1 1 391 88 477 1.04e-121 362
MS.gene35937.t1 MTR_7g087410 47.222 396 205 2 3 395 1 395 2.08e-121 358
MS.gene35937.t1 MTR_1g100180 46.329 395 210 1 1 395 100 492 9.54e-121 359
MS.gene35937.t1 MTR_8g063590 47.837 393 204 1 1 392 110 502 1.47e-120 359
MS.gene35937.t1 MTR_6g027200 50.643 389 190 1 1 389 94 480 2.58e-117 350
MS.gene35937.t1 MTR_3g099700 47.000 400 210 2 1 399 103 501 1.45e-116 349
MS.gene35937.t1 MTR_5g032750 46.667 390 206 2 1 389 93 481 2.52e-113 340
MS.gene35937.t1 MTR_6g027190 49.614 389 194 1 1 389 82 468 1.09e-110 333
MS.gene35937.t1 MTR_5g087930 47.395 403 187 5 1 399 83 464 2.76e-110 332
MS.gene35937.t1 MTR_3g099720 45.144 381 208 1 1 381 98 477 7.39e-109 328
MS.gene35937.t1 MTR_6g081410 42.308 390 223 1 1 388 82 471 5.79e-108 326
MS.gene35937.t1 MTR_7g087370 44.612 399 190 2 1 399 87 454 3.43e-107 323
MS.gene35937.t1 MTR_6g081780 42.526 388 221 1 1 386 82 469 1.04e-106 323
MS.gene35937.t1 MTR_6g066380 41.344 387 225 1 2 386 85 471 3.24e-102 311
MS.gene35937.t1 MTR_6g004220 39.447 398 241 0 1 398 81 478 5.47e-102 311
MS.gene35937.t1 MTR_6g047610 40.253 395 234 1 2 396 88 480 1.09e-101 310
MS.gene35937.t1 MTR_7g082810 42.857 385 220 0 2 386 78 462 1.86e-101 309
MS.gene35937.t1 MTR_6g004220 40.633 379 225 0 1 379 88 466 9.90e-100 305
MS.gene35937.t1 MTR_1g108810 41.579 380 222 0 1 380 91 470 2.20e-98 301
MS.gene35937.t1 MTR_6g081300 39.793 387 233 0 1 387 67 453 5.71e-97 298
MS.gene35937.t1 MTR_6g047640 40.609 394 232 1 2 395 89 480 9.77e-97 297
MS.gene35937.t1 MTR_6g081400 38.918 388 237 0 1 388 73 460 1.32e-96 296
MS.gene35937.t1 MTR_6g081240 39.314 379 230 0 1 379 67 445 1.26e-95 293
MS.gene35937.t1 MTR_8g106670 39.948 388 233 0 1 388 69 456 4.57e-93 288
MS.gene35937.t1 MTR_6g047640 39.086 394 219 2 2 395 89 461 8.84e-93 286
MS.gene35937.t1 MTR_6g081390 38.402 388 239 0 1 388 70 457 1.12e-92 286
MS.gene35937.t1 MTR_1g108840 40.155 386 231 0 1 386 98 483 1.70e-92 287
MS.gene35937.t1 MTR_1g108990 38.961 385 235 0 2 386 83 467 9.18e-91 282
MS.gene35937.t1 MTR_6g081810 39.740 385 206 2 2 386 85 443 1.14e-88 276
MS.gene35937.t1 MTR_6g081310 39.798 397 228 1 1 386 54 450 1.70e-88 276
MS.gene35937.t1 MTR_5g090280 40.566 424 217 4 3 398 1 417 2.22e-88 274
MS.gene35937.t1 MTR_6g047590 39.796 392 234 1 2 393 88 477 6.30e-88 275
MS.gene35937.t1 MTR_7g082800 36.788 386 244 0 3 388 1 386 1.60e-87 271
MS.gene35937.t1 MTR_2g078930 38.228 395 236 1 1 395 100 486 2.66e-86 271
MS.gene35937.t1 MTR_2g078960 37.845 399 239 2 1 398 90 480 6.12e-83 261
MS.gene35937.t1 MTR_1g109060 37.403 385 241 0 2 386 82 466 7.04e-83 261
MS.gene35937.t1 MTR_2g079020 37.215 395 240 1 1 395 94 480 3.92e-82 260
MS.gene35937.t1 MTR_1g109060 37.831 378 235 0 2 379 82 459 1.94e-81 258
MS.gene35937.t1 MTR_2g078970 37.722 395 238 1 1 395 88 474 4.13e-81 257
MS.gene35937.t1 MTR_6g004220 37.742 310 193 0 1 310 88 397 1.90e-71 231
MS.gene35937.t1 MTR_5g010830 29.243 383 271 0 2 384 123 505 3.23e-63 212
MS.gene35937.t1 MTR_1g101500 29.032 403 277 1 2 395 122 524 5.86e-59 201
MS.gene35937.t1 MTR_2g078990 35.599 309 191 1 87 395 3 303 1.31e-58 194
MS.gene35937.t1 MTR_5g067460 30.077 389 267 3 2 388 113 498 2.15e-57 197
MS.gene35937.t1 MTR_0039s0080 28.101 395 283 1 2 395 96 490 1.35e-55 191
MS.gene35937.t1 MTR_3g111020 29.049 389 271 3 2 388 109 494 8.30e-54 187
MS.gene35937.t1 MTR_6g043810 28.049 410 278 5 1 395 80 487 2.24e-51 179
MS.gene35937.t1 MTR_6g043810 28.049 410 278 5 1 395 90 497 4.21e-51 179
MS.gene35937.t1 MTR_6g004220 37.555 229 143 0 1 229 88 316 1.29e-50 173
MS.gene35937.t1 MTR_8g102430 27.297 381 257 2 2 382 81 441 1.26e-46 166
MS.gene35937.t1 MTR_6g088805 27.970 404 281 4 2 399 85 484 2.58e-45 163
MS.gene35937.t1 MTR_4g027920 25.065 383 285 1 2 382 214 596 6.08e-43 159
MS.gene35937.t1 MTR_2g079030 36.620 213 127 1 183 395 319 523 1.03e-39 149
MS.gene35937.t1 MTR_5g011540 29.146 398 273 5 2 395 101 493 1.60e-38 145
MS.gene35937.t1 MTR_2g435930 24.000 400 282 3 2 382 77 473 7.45e-32 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35937.t1 AT3G21690 77.215 395 90 0 1 395 108 502 0.0 612
MS.gene35937.t1 AT1G11670 68.170 399 125 1 1 399 106 502 0.0 571
MS.gene35937.t1 AT1G61890 67.168 399 130 1 1 399 103 500 0.0 561
MS.gene35937.t1 AT1G61890 68.158 380 121 0 1 380 103 482 0.0 538
MS.gene35937.t1 AT4G21903 62.907 399 146 1 1 399 108 504 0.0 523
MS.gene35937.t1 AT4G21903 62.720 397 146 1 3 399 1 395 0.0 518
MS.gene35937.t1 AT4G21910 61.905 399 151 1 1 399 112 509 0.0 514
MS.gene35937.t1 AT4G21910 61.905 399 151 1 1 399 112 509 0.0 514
MS.gene35937.t1 AT4G21910 61.905 399 151 1 1 399 110 507 0.0 514
MS.gene35937.t1 AT4G21910 61.905 399 151 1 1 399 110 507 0.0 514
MS.gene35937.t1 AT4G21903 61.990 392 149 0 1 392 108 499 4.85e-177 503
MS.gene35937.t1 AT1G61890 67.622 349 113 0 1 349 103 451 2.48e-172 490
MS.gene35937.t1 AT5G38030 51.880 399 191 1 1 399 101 498 3.81e-143 417
MS.gene35937.t1 AT1G47530 52.152 395 188 1 1 395 91 484 4.02e-141 411
MS.gene35937.t1 AT1G47530 51.908 393 188 1 3 395 1 392 4.38e-141 407
MS.gene35937.t1 AT3G26590 51.392 395 191 1 1 395 101 494 8.24e-140 408
MS.gene35937.t1 AT3G26590 51.145 393 191 1 3 395 1 392 9.46e-140 404
MS.gene35937.t1 AT1G12950 51.621 401 191 2 1 399 123 522 9.67e-139 406
MS.gene35937.t1 AT3G59030 51.139 395 193 0 1 395 107 501 6.24e-135 396
MS.gene35937.t1 AT1G23300 49.875 399 197 2 1 397 100 497 3.11e-130 384
MS.gene35937.t1 AT4G00350 47.558 389 202 1 1 389 144 530 9.53e-124 369
MS.gene35937.t1 AT1G33110 47.558 389 203 1 1 389 92 479 3.45e-118 353
MS.gene35937.t1 AT5G65380 46.076 395 211 2 1 394 93 486 3.03e-115 345
MS.gene35937.t1 AT1G33100 45.501 389 211 1 1 389 89 476 4.54e-114 342
MS.gene35937.t1 AT5G44050 45.340 397 213 3 1 394 96 491 4.86e-113 339
MS.gene35937.t1 AT1G33090 47.558 389 203 1 1 389 92 479 1.77e-112 338
MS.gene35937.t1 AT4G25640 47.000 400 207 2 1 397 90 487 5.35e-112 337
MS.gene35937.t1 AT4G25640 47.000 400 207 2 1 397 90 487 1.26e-111 337
MS.gene35937.t1 AT1G33080 46.310 393 210 1 1 393 92 483 1.00e-110 333
MS.gene35937.t1 AT5G10420 46.292 391 206 4 1 389 94 482 8.47e-109 328
MS.gene35937.t1 AT1G33080 46.335 382 204 1 1 382 92 472 1.42e-105 320
MS.gene35937.t1 AT5G52450 42.893 394 225 0 1 394 82 475 1.06e-101 310
MS.gene35937.t1 AT3G59030 51.935 310 149 0 1 310 107 416 6.66e-101 306
MS.gene35937.t1 AT3G03620 43.609 399 222 3 2 399 94 490 1.40e-100 308
MS.gene35937.t1 AT3G03620 43.609 399 222 3 2 399 94 490 1.40e-100 308
MS.gene35937.t1 AT1G73700 43.038 395 225 0 1 395 80 474 1.55e-100 307
MS.gene35937.t1 AT5G17700 42.784 388 221 1 2 389 91 477 2.43e-99 305
MS.gene35937.t1 AT5G17700 42.784 388 221 1 2 389 91 477 2.43e-99 305
MS.gene35937.t1 AT2G34360 40.000 395 229 2 1 394 83 470 1.77e-97 299
MS.gene35937.t1 AT4G25640 47.550 347 180 1 1 347 90 434 8.97e-97 297
MS.gene35937.t1 AT1G71140 38.442 385 237 0 2 386 83 467 2.28e-90 281
MS.gene35937.t1 AT2G04040 38.520 392 239 2 2 392 84 474 1.75e-89 278
MS.gene35937.t1 AT2G04070 38.974 390 238 0 2 391 84 473 1.85e-89 278
MS.gene35937.t1 AT2G04080 37.340 391 243 2 2 391 84 473 1.36e-87 273
MS.gene35937.t1 AT1G15150 38.144 388 236 2 1 386 87 472 1.42e-87 274
MS.gene35937.t1 AT1G66780 37.662 385 240 0 2 386 92 476 3.82e-87 273
MS.gene35937.t1 AT1G15160 37.629 388 238 2 1 386 87 472 5.71e-86 270
MS.gene35937.t1 AT2G04080 37.632 380 235 2 2 380 84 462 1.88e-85 268
MS.gene35937.t1 AT1G66760 36.181 398 254 0 2 399 85 482 5.18e-85 267
MS.gene35937.t1 AT1G15170 36.269 386 246 0 1 386 90 475 1.66e-84 266
MS.gene35937.t1 AT2G04100 38.083 386 237 2 2 386 87 471 3.22e-84 265
MS.gene35937.t1 AT2G04050 38.363 391 241 0 2 392 84 474 1.62e-83 263
MS.gene35937.t1 AT1G15180 38.083 386 239 0 1 386 91 476 2.36e-83 263
MS.gene35937.t1 AT2G04100 38.342 386 236 2 2 386 87 471 3.71e-83 262
MS.gene35937.t1 AT1G33110 46.624 311 165 1 1 311 92 401 1.74e-82 258
MS.gene35937.t1 AT1G66760 37.037 378 238 0 2 379 85 462 2.38e-82 259
MS.gene35937.t1 AT3G23560 37.666 377 235 0 1 377 95 471 1.02e-81 258
MS.gene35937.t1 AT3G23550 36.968 376 237 0 2 377 88 463 3.11e-80 254
MS.gene35937.t1 AT1G15150 38.028 355 216 2 34 386 4 356 8.84e-80 250
MS.gene35937.t1 AT2G04090 37.047 386 241 2 2 386 87 471 1.56e-79 253
MS.gene35937.t1 AT2G34360 34.772 394 209 1 1 394 83 428 1.85e-79 251
MS.gene35937.t1 AT1G64820 35.025 394 256 0 1 394 85 478 9.48e-79 251
MS.gene35937.t1 AT1G15170 36.416 346 220 0 41 386 11 356 6.80e-77 242
MS.gene35937.t1 AT1G66760 37.082 329 207 0 51 379 6 334 2.81e-69 222
MS.gene35937.t1 AT5G17700 40.064 312 186 1 2 313 91 401 4.03e-66 216
MS.gene35937.t1 AT3G23560 36.774 310 196 0 1 310 95 404 3.31e-59 198
MS.gene35937.t1 AT2G38510 27.089 395 287 1 2 395 63 457 1.73e-56 193
MS.gene35937.t1 AT1G66760 34.304 309 203 0 2 310 85 393 1.33e-54 186
MS.gene35937.t1 AT4G29140 29.134 381 270 0 2 382 113 493 3.66e-54 187
MS.gene35937.t1 AT1G15180 38.519 270 166 0 41 310 11 280 3.74e-54 185
MS.gene35937.t1 AT4G23030 30.175 401 276 1 2 398 90 490 5.47e-52 181
MS.gene35937.t1 AT5G52050 28.747 407 279 2 2 397 92 498 8.77e-51 178
MS.gene35937.t1 AT1G58340 27.708 397 286 1 2 398 115 510 1.01e-49 176
MS.gene35937.t1 AT5G19700 26.110 383 283 0 2 384 93 475 1.78e-46 167
MS.gene35937.t1 AT1G71870 25.547 411 287 3 1 393 80 489 3.18e-45 163
MS.gene35937.t1 AT5G49130 25.622 402 283 4 2 388 83 483 6.95e-37 140
MS.gene35937.t1 AT4G22790 26.010 396 287 4 2 393 87 480 1.43e-34 134
MS.gene35937.t1 AT2G04066 38.211 123 76 0 264 386 41 163 4.67e-26 103

Find 78 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGAAAATGATGCTGATTT+TGG 0.135593 2.1:+13549543 None:intergenic
CTCTTGATTCTCTTTCTATT+TGG 0.176770 2.1:-13555995 MS.gene35937:intron
GTGGGTTGTTATTATATAAT+TGG 0.203412 2.1:-13549186 MS.gene35937:CDS
GATTGCAGTACCACAGTTTC+TGG 0.211321 2.1:-13549932 MS.gene35937:intron
TAAGATTGGTTTGGGACTAT+TGG 0.284867 2.1:-13556252 MS.gene35937:CDS
TATCTTTGTAATAGGGAATA+TGG 0.290509 2.1:-13548057 MS.gene35937:intron
GGGACTATTGGGAGCTTCAT+TGG 0.301565 2.1:-13556240 MS.gene35937:CDS
CTCCCATTTGTTCAACCTCT+TGG 0.322256 2.1:+13547839 None:intergenic
GGCATTCAGCCTGTCCTATC+TGG 0.327784 2.1:-13549360 MS.gene35937:intron
AAGATTGGTTTGGGACTATT+GGG 0.331739 2.1:-13556251 MS.gene35937:CDS
CAAATGGGAGGATAAGAAAA+AGG 0.345023 2.1:-13547826 MS.gene35937:CDS
GAAGGGGTGGCTGTTGGTTG+TGG 0.345474 2.1:-13549237 MS.gene35937:intron
TATATAATTGGTATACCATT+GGG 0.346018 2.1:-13549174 MS.gene35937:CDS
ACAATTCTTCTAACAATAAC+CGG 0.368053 2.1:-13556515 MS.gene35937:CDS
TGGGTTGTTGTGTTTAAGAT+TGG 0.375412 2.1:-13556266 MS.gene35937:CDS
TCTCTTTCTCTTGTCCTCAA+TGG 0.376120 2.1:-13549381 MS.gene35937:CDS
TTATGCTTTGTTTAGAGTCT+TGG 0.377854 2.1:-13556070 MS.gene35937:CDS
TGTTGTGTTTAAGATTGGTT+TGG 0.379266 2.1:-13556261 MS.gene35937:CDS
GGACTTGGAATGGGTTTACT+TGG 0.387809 2.1:-13556145 MS.gene35937:CDS
TGAAGAGTGAAAAGTGTAAG+AGG 0.391926 2.1:-13556166 MS.gene35937:CDS
GTGAAAAGTGTAAGAGGACT+TGG 0.399498 2.1:-13556160 MS.gene35937:CDS
CAGCCAAGAGGTTGAACAAA+TGG 0.408736 2.1:-13547842 MS.gene35937:CDS
GTTGTGTTTAAGATTGGTTT+GGG 0.415091 2.1:-13556260 MS.gene35937:CDS
ACTGTTGTTACAGCTGGGTA+TGG 0.420426 2.1:-13556618 None:intergenic
GCTGCTGCAGAAGCTATTCT+TGG 0.429389 2.1:+13556431 None:intergenic
GTGATTGTCCAATGAATACT+AGG 0.437699 2.1:+13556453 None:intergenic
CTGCATAAGCAAATATTTGT+GGG 0.438528 2.1:+13556387 None:intergenic
GGGTGGCTGTTGGTTGTGGA+TGG 0.451879 2.1:-13549233 MS.gene35937:CDS
TTATATAATTGGTATACCAT+TGG 0.453490 2.1:-13549175 MS.gene35937:CDS
ACTGCATAAGCAAATATTTG+TGG 0.462026 2.1:+13556386 None:intergenic
ACACTATGTGGACAAGCATA+TGG 0.467239 2.1:-13556581 MS.gene35937:CDS
AGTGTAAGAGGACTTGGAAT+GGG 0.479485 2.1:-13556154 MS.gene35937:CDS
ACTTCTTCCTCAACCTGAGT+TGG 0.483225 2.1:-13556018 MS.gene35937:CDS
TTCTTGGTGGGTTATTGTTG+TGG 0.488236 2.1:-13556207 MS.gene35937:CDS
GAACCAATCCTAGTATTCAT+TGG 0.496824 2.1:-13556461 MS.gene35937:CDS
TAGTATTGGATTTACCAGAT+AGG 0.497028 2.1:+13549346 None:intergenic
TGTCCAATGAATACTAGGAT+TGG 0.506736 2.1:+13556458 None:intergenic
TGCAGACAATTATTCTAATG+TGG 0.511947 2.1:-13548009 MS.gene35937:CDS
GGAATATGGCTAGGAATGCT+TGG 0.518781 2.1:-13548043 MS.gene35937:CDS
GTGAGAGTGAGCAATGAACT+AGG 0.519049 2.1:-13549576 MS.gene35937:CDS
TCAAGAGCCAACTCAGGTTG+AGG 0.534588 2.1:+13556011 None:intergenic
CATTAGAATAATTGTCTGCA+TGG 0.535846 2.1:+13548012 None:intergenic
AGCCAAGAGGTTGAACAAAT+GGG 0.536702 2.1:-13547841 MS.gene35937:CDS
TGCTATTACTGATATGAGAA+AGG 0.539452 2.1:+13549500 None:intergenic
CTGTTGTTACAGCTGGGTAT+GGG 0.540112 2.1:-13556617 None:intergenic
TGGGTGTTCATGATCTCAGT+TGG 0.543107 2.1:-13549908 MS.gene35937:CDS
GCAGTACCACAGTTTCTGGA+TGG 0.544262 2.1:-13549928 MS.gene35937:intron
AAGTGTAAGAGGACTTGGAA+TGG 0.545125 2.1:-13556155 MS.gene35937:CDS
GACTTGGAATGGGTTTACTT+GGG 0.549121 2.1:-13556144 MS.gene35937:CDS
TTTGTTGCTTATGTGAATGT+GGG 0.550454 2.1:-13549204 MS.gene35937:CDS
CCAAGTGCATACATTTCAGC+AGG 0.560592 2.1:-13556317 MS.gene35937:CDS
GGGTCACATTTAGAACGGAT+TGG 0.566170 2.1:-13547988 MS.gene35937:CDS
ACAAGAGAAAGAGAGAGAAG+TGG 0.566712 2.1:+13549390 None:intergenic
TTGGAATGGGTTTACTTGGG+AGG 0.571625 2.1:-13556141 MS.gene35937:CDS
TAAATTGTCGGCCGCGTCGG+CGG 0.571641 2.1:-13556093 MS.gene35937:CDS
GCAGACAATTATTCTAATGT+GGG 0.575732 2.1:-13548008 MS.gene35937:CDS
AATGTGGGTCACATTTAGAA+CGG 0.576394 2.1:-13547993 MS.gene35937:CDS
CAGTACCACAGTTTCTGGAT+GGG 0.577625 2.1:-13549927 MS.gene35937:CDS
TTGTAATAGGGAATATGGCT+AGG 0.578144 2.1:-13548052 MS.gene35937:intron
GGACAGGCTGAATGCCATTG+AGG 0.580911 2.1:+13549367 None:intergenic
TTGGATTTACCAGATAGGAC+AGG 0.583261 2.1:+13549351 None:intergenic
AGAGAATCAAGAGCCAACTC+AGG 0.586693 2.1:+13556005 None:intergenic
CCTGCTGAAATGTATGCACT+TGG 0.594920 2.1:+13556317 None:intergenic
ATATAATTGGTATACCATTG+GGG 0.597799 2.1:-13549173 MS.gene35937:CDS
ATGAATGAAGGGGTGGCTGT+TGG 0.602749 2.1:-13549243 MS.gene35937:intron
TTTCACTGAGGGTGAAGTGG+TGG 0.605190 2.1:-13549436 MS.gene35937:CDS
ATTTAGAACGGATTGGAACA+AGG 0.605847 2.1:-13547981 MS.gene35937:CDS
TTGGATTCAATGCAGCTGCA+AGG 0.606959 2.1:-13549889 MS.gene35937:intron
ATATGGCTAGGAATGCTTGG+TGG 0.618583 2.1:-13548040 MS.gene35937:CDS
AGTGCAGTTGAGACACTATG+TGG 0.629032 2.1:-13556593 MS.gene35937:CDS
TAGAATAATTGTCTGCATGG+TGG 0.647323 2.1:+13548015 None:intergenic
TATAATTGGTATACCATTGG+GGG 0.652825 2.1:-13549172 MS.gene35937:CDS
ACAAAGCATAACCGCCGACG+CGG 0.667117 2.1:+13556082 None:intergenic
CAAGAGGTTGAACAAATGGG+AGG 0.683158 2.1:-13547838 MS.gene35937:CDS
GAACACCCATCCAGAAACTG+TGG 0.691689 2.1:+13549922 None:intergenic
TTAATACGTAAATAACTGTG+AGG 0.695351 2.1:+13556486 None:intergenic
AGGTGAAAGAAGCAGCCAAG+AGG 0.696202 2.1:-13547854 MS.gene35937:CDS
TAGAACGGATTGGAACAAGG+AGG 0.755260 2.1:-13547978 MS.gene35937:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.1 gene 13547809 13556627 13547809 ID=MS.gene35937
chr2.1 mRNA 13547809 13556627 13547809 ID=MS.gene35937.t1;Parent=MS.gene35937
chr2.1 exon 13555996 13556627 13555996 ID=MS.gene35937.t1.exon1;Parent=MS.gene35937.t1
chr2.1 CDS 13555996 13556627 13555996 ID=cds.MS.gene35937.t1;Parent=MS.gene35937.t1
chr2.1 exon 13549890 13549946 13549890 ID=MS.gene35937.t1.exon2;Parent=MS.gene35937.t1
chr2.1 CDS 13549890 13549946 13549890 ID=cds.MS.gene35937.t1;Parent=MS.gene35937.t1
chr2.1 exon 13549361 13549599 13549361 ID=MS.gene35937.t1.exon3;Parent=MS.gene35937.t1
chr2.1 CDS 13549361 13549599 13549361 ID=cds.MS.gene35937.t1;Parent=MS.gene35937.t1
chr2.1 exon 13549137 13549255 13549137 ID=MS.gene35937.t1.exon4;Parent=MS.gene35937.t1
chr2.1 CDS 13549137 13549255 13549137 ID=cds.MS.gene35937.t1;Parent=MS.gene35937.t1
chr2.1 exon 13547979 13548065 13547979 ID=MS.gene35937.t1.exon5;Parent=MS.gene35937.t1
chr2.1 CDS 13547979 13548065 13547979 ID=cds.MS.gene35937.t1;Parent=MS.gene35937.t1
chr2.1 exon 13547809 13547874 13547809 ID=MS.gene35937.t1.exon6;Parent=MS.gene35937.t1
chr2.1 CDS 13547809 13547874 13547809 ID=cds.MS.gene35937.t1;Parent=MS.gene35937.t1
Gene Sequence

>MS.gene35937

CTGGGTATGGGAAGTGCAGTTGAGACACTATGTGGACAAGCATATGGAGCAGAAAAATATGACATGCTAGGAACATATTTACAAAGATCAACAATTCTTCTAACAATAACCGGTTTTTTCCTCACAGTTATTTACGTATTAAGCGAACCAATCCTAGTATTCATTGGACAATCACCAAGAATAGCTTCTGCAGCAGCACTTTTTGTGTATGGACTTATCCCACAAATATTTGCTTATGCAGTAAATTTTCCAATCCAAAAATTTCTTCAAGCACAAAGTATAGTTCTACCAAGTGCATACATTTCAGCAGGAACTTTAGTTTTTCATCTAATATTGAGTTGGGTTGTTGTGTTTAAGATTGGTTTGGGACTATTGGGAGCTTCATTGGTTTTGAGTTTTTCTTGGTGGGTTATTGTTGTGGCACAGTTTATTTATATTTTGAAGAGTGAAAAGTGTAAGAGGACTTGGAATGGGTTTACTTGGGAGGCGTTTTCGGGGTTGCCGGAGTTTTTTAAATTGTCGGCCGCGTCGGCGGTTATGCTTTGTTTAGAGTCTTGGTATTTTCAGATTTTGGTTTTGCTTGCTGGACTTCTTCCTCAACCTGAGTTGGCTCTTGATTCTCTTTCTATTTGGTAAAGTATCTCAACTTTGTTTTCTTCTGTTTTTCTTACTTTATTTTTTTTAATTTATGATTCATAATTAGTTGAGATTTCAGAGTTTCGCTGTTTTACCTTAGAAATGTTTGAGTTGTTGGTGAAATTATCAAAATTTTAAGGATTTTCCTCTGTAAAATTTGTGTCTTTATTTAAGGAGTCAATGTGACTTTGAGTTGACTTTGTATGCATTGAAACGTATTGAATTTATGTTGATAAAAAATGTGATGATGAAAACGCAAGATGCATGTGAAGGGAAGGTTGAGAAACAAAACAATATGACTTTAATATCACAATGTGATTTTTGGTTTCATAAATGACTGGTCAATACTAGTACTAGGTATCTGTAAAGAGCAATGCTATATCTCAAGAAAAATAACATCAAAAATTCTTCAAGAATAAAGAGCAGCCAATCACAATGCACTATTTACCACAATTCAAGGAGGTGTGGCTGAAAAATAGGAAGTGAACAAATAAATCATGCAATTAAAACTTGCCAACTGTGTCTTGAAAAATTCTTGATGCTATTTTTCTAGATAACTAGCATTTTCCAACTGTAAAAGTAACTTATATATGTGGTCCTTGCTCCTTACTCAATATGTTACTGATTTTCCTTGAGGAAAATAAGGAAAATTATCATTCAAAGAATCAAATTAGGGAATTTGGGATTTGCACACAATATAACTGATTTAGTGATGCAGCAGATAGACACATTTATTAGTATAACTAAAGTGATTATAGTATGTCTTTTCTTGAGTATGCCACTACAAGAAAAACACTGATCTGGGACGGAATATTCCGTCGCACAATAGTGTAAATATCTGTCCCAATTCAAATCTGGGACGGAATTTGCGACAATTTACTTTCGTCGCAAATAGTGCTGTCGCAATCAGTTGGGGACGGAATTATTTCGTCCGTCACATATTTGGGACGGAATGTGGGACGGAATGAAATTATTTGTTTTTTTAACACTTCTTTGTTTCCGTCCCAAGGTTGCGACAGAATACTTTTATTCCGTCCCAATGCAGTGACGGAGTTTGGGACAAAATGAAATTAATGTAAATCTGATTTATTTTTTAATATATCTTTTGGGACAGAATTATATAAATATTCTGTCACAAATGAATTTTATGTAATTTTTAAAATTCTCCCAAAAATTTAATATATATTTATATGATGCACATTAACTTGTGTCTATCATCAAGTTCCAAATTTGATACTACAAATCATTAGTAAACATAAATTGTTTCATCATTAACTTAGCACATAACCATCCAAAAAACAACATATATCAAACTATTTTCTCAACACCCAAAATGGTTAAAGTTATAATAACTAGAAAGTGCCTACAAAATATCAAATTGTTTCCTACATTCATAAACCATCAATATTATTCTTCCACATCAGCACCAACATTTGCATTGCTTCCACAAGTAGCATTATCAACAGAGTGATCATCTTCATCTAAATCCATTGAATCCAAATCTATATTTGCATTTTTAGAAAATGCAGCAATCATTTTGTTTTGACGAGCCATAATGGAATTGATTGTTTGTTGTTGCTCTGCAATTTTTTGCCTTAATCGCGCCTCAACCTCTCCACTCATACCACGAGCAATATCAGTATAATTTGAATATTTATTGGATTTAGACGTTGAGCCACGGGTGCGCATGCTGCCATCCAAACCAAGCCCATATACTCCACCCTTTGCATTTCTTCCTCCAACAGCTTCAATCATTAGTGTATCATAATCAATAGGCTTGGGTTGAGTACCACTCTCATTATTACCAACCTCAGAAGGATGAGGTTGAGACTCAACTTGTGAACAGAGATTAACAAAGTTTTTCTGAAAAATATATAACTGAATAAATAAGATAGTCTAACAAGCAACACAGTTTAACAAGGTAAATTAAGGAGGGAACACTGCATTGTACCTAAACAGAACATCCCAACAAACCAAATTATAAAGGCAATACATGAGTCCTATAACAAGTCATTCGATTACTAAAAAACAACTACATCAACCTAATACATTATTAGATTTATACAATCTATCCAAACCACCAGTACTGCAATAACAAAAAAACACCAAACATAAATGCAAGCCAAAAACTAAAGTAGATAAAAGGCCACAGTCGCCGATAATATATTTGTCAAACACGCACAAAGTTGATGCTCAAAACAGAATCACGGCCGCATGGAGAAAAAATAAACATACCGTAGTGCATTTCAGCAGTCTAAAACAGACCCAAAAGATAAACTACAAGTCTCGACTTTTTTTCTACTTACACAAACCAAGTAAAATTAAAGAAGCCAATAACTTGATACTCACATATGCATCAGTTGATCTCTTATCAACAAAGGTTTGTGACCCCTTCCATTTGGGTTTTTCTGACACTTTTTCACATACTCGAGCCATTATCTTTCTTAGTAGATCCTTAGCTTTTGAATCCCATGTGGCCCTAATTGTTGCCTCTTGTTCACCAATCCATTTGTAGTTTTTCTGCAATTATTAAAAATGGGAATAGAAAATTCAATTTAAAACAGTTCAAAGTTACAACACTATGAAAAGATAACAAATAACCAAGATGCATTACCGCCCACTCATTAAACCAAATTTCTTTTATTTTATCGTTGCAACATTTCCAATTGTTGTAAGGGCCATCAAACATAGATTTAATTGCCTTCGATATATCTTTAGTCTCGTTATCATCAGGATAAAGGCTGCCAAATTCAACACATGAATTAACAAATTAAATAAAAAAGATTATATAATCTACAATAAATTAAAACTGAGAACTATACATGCATATTAAATTAAATAAACAACTTGACTTACTATCCTTTAAGAGTATAAATCCAAATTTTGTTATCAGTAAGATCTTTGCCCCATGGTAGATGTGGTTTATGAGGACCAGGTAAGGTAGGGTCTCCAAATGGGATAAAATTTTCAGGTACATATATATCAGTAAAAGTTGATGACAGTATCAGTAAAATTTTGTTATCAGTAAAATTTTCAGGATCTTTGCCCCAAATTTTTTTACGTGCCATGTCTAAAAGTTGATGACAATGGCCAACATACATATCTATCTGAATCAACAGTGAAGCTAAATCCCACCGAATTTGTACATATATTATTGACACTCACGGTCGAAGTTAGATGATAAGGTATGTATAGGTAATAACTCCAAGAGTATCACATAATCTCATCATTCAATTGGACAAACATATTAGACAAAATACAAACAGCTATTTTGACTTCAATCTGTCTATAACAGACAAAACAAACCACACTCAGTACTTAATTTAGAAAATGCATAAGAAAATGCATTCAAAAGGTACCTTCAATCTTAATGATAATGCTTGCTTACTAAAATACCCAAGACACTAAATGCAGCAAATGAAGCAACATAGAAAAACCAAAACAAAGAAAACAAACCCATTTCATAATTTCAACAAACCAGGTTCAAATTTTCATTAAAGAGAAACATAATTTCAACAAAAATCAAAACAAACCAGTACAAAAATGATTATTACATGGGGTAAAGCACCATTAACCTTTCAACCAAAAAAAATTATTATTGTGCTCCAATCTTTAAACTCTCATTCATGATAAAGTTAATCAATACCCTATTGTTACCTTCAAACTCACATTCAAAGTATAAGCAAACAAACACACAAGCTCACCTCTTAGAAAAAACAAGCAAAACTTTGATAAAACTATTTTGACACAGAGTAGAAAGAATAAAAATCATTGTTGCATTTTTAGAAACATGTTAGGAAAAACAATTATGACAGGGTATTGATACCATCAACTATGTACTAAGCCATCAACATCATCCTCATCAGAAAGATTTTCCTGATTTTCTACCAACATTAAGTATTTCTAATAATAATGACAGAGGAGCTATAAAACAGAGCTTCAATGGAGAAAGGAGATTTCACATTAACATCAACTTACATGAAAAACTTATCAACAAGTGCTGAACTATTACTTAGCTAATTCTTAATAAAGATTCAGCATTGCTAGCTTTCAAAATGATGTATTTTTTATGTTAATCTTAACTATTTCTTTAATTGCCAACAAGTGAGTAATGCAAATTATTGCATCAGAAAAAAGATTAAATGTGGTGATTATTACTACCACTCTATGTTGAACTAACACAATTCATGTCTTAAATAAGTAAATCATAGCTTGAATATCAATACACATTAAGTATTGCACATGTTAAGATATCTAAAAGTGTTTTTCTGTTGTATTCTACTAAAATGATTACTATTTTGCTACAACATTCTGGAAAAAAAAAATCACTTTTCTGCTGCAACCTTCCGACAAAAAAAATCATTTTTATACTGCAACATTCAAACCCACATCTAGGACAAAATAAAACAATACTTTTCTACAACCTTCAAACTCACATTCAACATACTCTTTTACCAAAAATCAAATTTCAACAAACCCATTTCATGAAAATCAAAAAATTCATTAAAGAGAGACAAGTTAACAAGCTGAGAAAGAAATGAAATTTCAAACCTGAAATTGCACAGAACTGTGAAGGAAAAGCGATTTCTGATTGAACTGAGAAGGAGAAAGAACCTGGACTGAGAAGGAGGAGCGATTGTGAACTGAGAAGGAGAAAGAACCTGGACTAAGAAGGAGGAGCGATTGAGAGAGAGAGAGAGAGAGAGAGTGAGTGAGAGATGGAATTTAGGGAGGGTTATGGCGGGTTATCATCATCTTCAGATTACTGCGAAAGAGCTTTGAGAGGGAGAGCGATTTAGGTTTTCAGATGGATTGTGTTTTTACGGAACCGTGTGATAAGCAAGATGGATTGTGTTTTTGTTTTTGTTTATTGTTACAGATGGATTTGTTTTGTACGTATATAGATAAGGTTGGATTCTGTTGATATTATTTTTAATTATCTTAATCTTATTTTTGAAGAAAAATAATAAATAATATTAAAAATAACAAATAATTTTCCCGCCCACGTTATTCACCAAAAAAATATTCACTCCAACAATTCTCACAAATTTTTTGGATTAAAATAGACCAAGTTGTGACAGAAATAAAAATTCCGTCCCAAATTTGTGAAAAAAATATTAGTTCCGTCCCAAATTTGCGACAGAAATATTAATCCGTCCCAAAAATATATATAAATTAAAACAAAATATTTGTAACACAGAAGCGTCGCAAATTCCGTCCCGATTTAATTAATTTGGGACGGAAATAAAATTCCGTCACGACGTTCCGTCTTAATTTGGCTGTTTTCTTGTAGTGTGCCCCATATTTTTGGCTTCTTGTTCTTTATTCAGCCAGCACAAGGAAAACTACATAATATAGTGTCACTTATATATAAATTCAAGTGTATGTATACCATTATTCTGCAATACCATGTGCACAATCCTTATCCTTATCACTACCAAAGGGATGCACGCAAAATGGTTAGGCTAAGATCAAATAGGAAAAAAAACAAAGAAGATATGGAAAAAAAAAGAATACTGCTCAAAAGTTAAAATTGCACACTCTATGTTTTCTTTTGTTGATATATAATTTATATAATCTCCCCCATCATGACTAGAATGACACAAATTAATAAACTTGTCCTGTTATTCAGATATGGGGCCATTGGAATTAAGTGCCTGTTTTGTTTTAACCATACTATCACATGCCACCTTTTAATGTCACGTGAGGAGATTCTAACTTGTAAGGTTTTAGGTTCGTTCAATGTTTGGTGTTAATTTGTGATAGTCATACAAAACGAATGGACACCGTCGTTAATATTGTGGTCCAATAACCAATTTGTTCTGGCGCAATTATTGATAACATGTTTGTGATCTTTGTATATTGAAAAATGCTAAATATAGCGACTCTTTCTTTGACGAATGTTGTTGTTTATTTTGGTGAATTCATGTGTAGTCTAGAATATATAATATTATATTCTTATGAAAAAAAAGTATATAATATATTAGATACACACGTACACAAACAAGTTTTCATTTGTTTGTTTGTTACTATTTGTGATTGCAGTACCACAGTTTCTGGATGGGTGTTCATGATCTCAGTTGGATTCAATGCAGCTGCAAGGTTAGACTCTAGTCAATTAGTTTACAAATTTTGACAAACTTGAACATACATATATTATTTGAGTTACCTACCATCGGCACTTTAGGTCGAAGATATGTTTGAGTGTCTAACATGTGTCGGTATCTGATACGACACATGCACTAAATAACACGCTAGCGTTCATGTGTCAATGTAGTCAGTATATTGTTTTTTTTACTAAATAATGTAGCTAGTATATGGTTATATAACTGAAATTGTACTAAATAACCTGATATTAGACATTTTATTAATTACATAATTAACATGTGCAGTGTGAGAGTGAGCAATGAACTAGGAGCAAGAAATCCAAAATCAGCATCATTTTCAGTGAAAGTGGTGACATTGATATCCTTTCTCATATCAGTAATAGCAGCACTGATTGTGCTTGCATTAAGAGATGTTATTAGCTATGTTTTCACTGAGGGTGAAGTGGTGGCTGCTGCTGTCTCAGATCTTTGTCCACTTCTCTCTCTTTCTCTTGTCCTCAATGGCATTCAGCCTGTCCTATCTGGTAAATCCAATACTACTTAATTACTGATTTTGTTTGTTTATTATTAATTACCCGGTTAATTAGTAACTAATTGAATATACATATATTAATTTTACATGAATGAAGGGGTGGCTGTTGGTTGTGGATGGCAAGCTTTTGTTGCTTATGTGAATGTGGGTTGTTATTATATAATTGGTATACCATTGGGGGCGGTTCTTGGCTTTTATTTCAATTTTGGTGCTAAGGTATGTTATTTATGATTATTTTTTATTTATTTTGGAGTAATAGGTGTTTCTGTATATTCAATTTAAGCAATTTCTAATGGTGTTTTACTAAAAGTTGAGAAAAAGATTTTTTTTTTAATTAATTAAAATGACATCGATGATCAATTTACCACTTTTTCCAATAAGATATGGACTTCATCCTATCTTGAAGGAATTTGAACTTCATCTTTAGAATGTTAGGTTAGAACGTCAAACTCTTACAACATGTGAAGCCCAGATACGAGATACGATACGGATACGATACTGCACCGATACGTCGATACGGAAAAATTTCAAAAATCAAGATACGATACGGCTGGGATACGTTAATAAAAAAATTTATATAATTAAATGTACAATATAATTATAACCATTTTTTTAATATGCAAATATATTAACAAACAAAAGAAACCAGTCATAGACTCATAGCACATCAACATAAATATAAATAATCTTGACGATTAAGAGAGTGATGATTAGTTAATTACATAAACAATATATCGCAAAAAGAGCACAATTAGTGCTTTATTATATCCTAAAGGAATATTGTTAGTGTTATACTATATCTATCCAAAAAAGAAACATTATATCCAAAAAAGAACACATCAGTACTCAAAAGTTAAGTTATTTTAGTTAACATTAATTGGGAAATATTGGGGCAGGTATCAGTGCAGGATAATTATCGGATACTCTCCTGATATGTATCAGGAAGTATCGAATAATTATTTTTAAAAAAATAAAATTTAATCTATGGATACTCTTCTGATACGTATCGGGAAGTATCGGGCATGTATCGGTGCAGGATACGCAGGGGATACGATACGTCATCCATTTCGAAGTATCGGGGCTTCACAACTTACAACTAAATTAGTAACTCAGGAACTTCTAGTCTTCAACACATGTTTAATTTTTTGTATATGTATGGTATCTGCTATATATATATATATATATATATATATATTTTAACTGAAAACAAATACATTTCATTCTATCTTAGTCATAAAATTTAAAACATATAAATATTATTTTCTTAATGGCTTATCTTTGTAATAGGGAATATGGCTAGGAATGCTTGGTGGAACCACCATGCAGACAATTATTCTAATGTGGGTCACATTTAGAACGGATTGGAACAAGGAGGTAAAAGACAACTTTGCAATTATTTCATTTCTATGATGACACTGTGCAGGGAATGGGAGAATCATTGTTATTGTGTCTAATAAAAAAATATGTCTTCTTTGTAGGTGAAAGAAGCAGCCAAGAGGTTGAACAAATGGGAGGATAAGAAAAAGGAGCCCCTTCTAAACTAA
Protein sequence

>MS.gene35937.t1

LGMGSAVETLCGQAYGAEKYDMLGTYLQRSTILLTITGFFLTVIYVLSEPILVFIGQSPRIASAAALFVYGLIPQIFAYAVNFPIQKFLQAQSIVLPSAYISAGTLVFHLILSWVVVFKIGLGLLGASLVLSFSWWVIVVAQFIYILKSEKCKRTWNGFTWEAFSGLPEFFKLSAASAVMLCLESWYFQILVLLAGLLPQPELALDSLSICTTVSGWVFMISVGFNAAASVRVSNELGARNPKSASFSVKVVTLISFLISVIAALIVLALRDVISYVFTEGEVVAAAVSDLCPLLSLSLVLNGIQPVLSGVAVGCGWQAFVAYVNVGCYYIIGIPLGAVLGFYFNFGAKGIWLGMLGGTTMQTIILMWVTFRTDWNKEVKEAAKRLNKWEDKKKEPLLN