Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36047.t1 | XP_003628552.1 | 97.5 | 526 | 13 | 0 | 1 | 526 | 1 | 526 | 1.30E-281 | 978.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36047.t1 | Q16401 | 21.1 | 460 | 325 | 13 | 55 | 507 | 62 | 490 | 1.4e-10 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36047.t1 | G7LDS0 | 97.5 | 526 | 13 | 0 | 1 | 526 | 1 | 526 | 9.5e-282 | 978.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050877 | MS.gene36047 | 0.808492 | 3.02E-50 | -1.69E-46 |
| MS.gene053542 | MS.gene36047 | 0.802192 | 6.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36047.t1 | MTR_8g061280 | 97.529 | 526 | 13 | 0 | 1 | 526 | 1 | 526 | 0.0 | 1045 |
| MS.gene36047.t1 | MTR_8g058790 | 97.529 | 526 | 13 | 0 | 1 | 526 | 1 | 526 | 0.0 | 1045 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36047.t1 | AT3G15180 | 55.577 | 520 | 226 | 3 | 7 | 526 | 5 | 519 | 0.0 | 566 |
| MS.gene36047.t1 | AT3G15180 | 52.536 | 552 | 225 | 4 | 7 | 526 | 5 | 551 | 0.0 | 553 |
Find 99 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTGCTTTCATCAATAATT+AGG | 0.242205 | 8.1:-52567447 | MS.gene36047:intron |
| AGTTGTCTACCATGCCTTTC+TGG | 0.271925 | 8.1:+52562399 | None:intergenic |
| TTTGCAGGTCACTTCCCTTC+TGG | 0.285703 | 8.1:-52571423 | MS.gene36047:intron |
| CTTGAATCGTTGGGTCAAAT+AGG | 0.290113 | 8.1:-52565301 | MS.gene36047:CDS |
| TTAAATGTCTCACCGCTATA+CGG | 0.368585 | 8.1:+52557064 | None:intergenic |
| AGGTTATTCAAAACAAAATT+CGG | 0.377036 | 8.1:-52573782 | MS.gene36047:CDS |
| TTGCTTGATTGCCTTATCAA+TGG | 0.380041 | 8.1:-52571335 | MS.gene36047:CDS |
| TGGTGTCAGCTTTGAGCTCC+TGG | 0.386617 | 8.1:+52558081 | None:intergenic |
| GTATCACCTCCTTTGGTAGA+TGG | 0.389521 | 8.1:+52568772 | None:intergenic |
| GTCTCGACCGTGGTGCTTGA+TGG | 0.390375 | 8.1:-52556455 | MS.gene36047:CDS |
| TCGAGTTCGTGTATTGGCTT+TGG | 0.396063 | 8.1:-52568435 | MS.gene36047:CDS |
| ACCAGGGTAGTTGGCGAAAT+CGG | 0.401067 | 8.1:+52574205 | None:intergenic |
| TATCTACTTACCCATTGATA+AGG | 0.403257 | 8.1:+52571324 | None:intergenic |
| TTAGGGTCGAGTTCGTGTAT+TGG | 0.409795 | 8.1:-52568441 | MS.gene36047:intron |
| TGCTTGATTGCCTTATCAAT+GGG | 0.420789 | 8.1:-52571334 | MS.gene36047:intron |
| TAGCTGCTTGAAGGAGTTGT+TGG | 0.422593 | 8.1:+52574228 | None:intergenic |
| CTATCAGCCGTCATCACAAT+AGG | 0.428327 | 8.1:+52555957 | None:intergenic |
| TTCTCTCATTCCACAGTACA+TGG | 0.435662 | 8.1:-52573756 | MS.gene36047:intron |
| AAGGTCTGTATCACCTCCTT+TGG | 0.437675 | 8.1:+52568765 | None:intergenic |
| AGAAACGAAGAGGTGGCAGC+TGG | 0.443137 | 8.1:-52568973 | MS.gene36047:CDS |
| TATGACGTCATTCTCAGATC+TGG | 0.444701 | 8.1:+52558156 | None:intergenic |
| CGAAATCGGTAGCTGCTTGA+AGG | 0.448609 | 8.1:+52574219 | None:intergenic |
| AGCTGCTTGAAGGAGTTGTT+GGG | 0.449722 | 8.1:+52574229 | None:intergenic |
| TGAAACTGCACTTGAATCGT+TGG | 0.452928 | 8.1:-52565311 | MS.gene36047:CDS |
| TGAGGTTAGCGACGATTTCA+AGG | 0.456722 | 8.1:-52567343 | MS.gene36047:intron |
| CGAAAACACTTAAAGTTACA+AGG | 0.462832 | 8.1:+52568312 | None:intergenic |
| TGAGGGTAGACTTCAGTCCT+TGG | 0.465415 | 8.1:-52565356 | MS.gene36047:CDS |
| AATTGTTATTTCATCCATTG+AGG | 0.467650 | 8.1:-52565374 | MS.gene36047:CDS |
| GTTCAGCAATTTGTTCAAGT+TGG | 0.473635 | 8.1:-52573647 | MS.gene36047:intron |
| TTTCATAAAGTAAGCGAAGC+AGG | 0.475520 | 8.1:+52558110 | None:intergenic |
| CTGCTTCCAACAGTGTAAGT+AGG | 0.479841 | 8.1:+52568357 | None:intergenic |
| TCAAATTCATCTTTATTTGA+AGG | 0.481539 | 8.1:+52565331 | None:intergenic |
| GCAGCTGTTCCTGCAAAAGC+AGG | 0.484395 | 8.1:+52556167 | None:intergenic |
| CTTACATGGTTCATCTATGA+AGG | 0.486485 | 8.1:+52567254 | None:intergenic |
| CAGACTCAATTTGATGTACC+TGG | 0.486714 | 8.1:-52573836 | MS.gene36047:CDS |
| GGACTGAAGTCTACCCTCAA+TGG | 0.487252 | 8.1:+52565360 | None:intergenic |
| AAGTAGGTTTAAACTGCATA+TGG | 0.488548 | 8.1:+52568373 | None:intergenic |
| ATTTGTTCAAGTTGGTCTGC+AGG | 0.489346 | 8.1:-52573639 | MS.gene36047:CDS |
| CAGGGTTATAGAATGATAAG+TGG | 0.493664 | 8.1:-52556484 | MS.gene36047:CDS |
| CAAATTCATCTTTATTTGAA+GGG | 0.498349 | 8.1:+52565332 | None:intergenic |
| TCAAGAGCAATGATGATAAG+TGG | 0.498760 | 8.1:-52567306 | MS.gene36047:CDS |
| AGAAAGGCATGGTAGACAAC+TGG | 0.499718 | 8.1:-52562397 | MS.gene36047:intron |
| ACTAGTGTAGTTTGTAGGCC+AGG | 0.500843 | 8.1:+52573818 | None:intergenic |
| ATCTTTATTTGAAGGGTCCA+AGG | 0.502106 | 8.1:+52565339 | None:intergenic |
| AACTTGATAATGAGTGGAAG+AGG | 0.503962 | 8.1:+52574043 | None:intergenic |
| TTGATGAAAGCAGTTGAAAG+AGG | 0.509011 | 8.1:+52567455 | None:intergenic |
| ATAAGGCAATCAAGCAAAAG+TGG | 0.509511 | 8.1:+52571341 | None:intergenic |
| AGTTCGTGTATTGGCTTTGG+TGG | 0.509973 | 8.1:-52568432 | MS.gene36047:CDS |
| CTGCAAGAAGCCGTAGGAAT+GGG | 0.511865 | 8.1:-52556017 | MS.gene36047:CDS |
| ACAGAACTAGTGTAGTTTGT+AGG | 0.515735 | 8.1:+52573813 | None:intergenic |
| CAGGCTGCACTGCATGCTCT+AGG | 0.517824 | 8.1:-52558196 | MS.gene36047:CDS |
| GAAACTGCACTTGAATCGTT+GGG | 0.520239 | 8.1:-52565310 | MS.gene36047:CDS |
| ATTGTTATTTCATCCATTGA+GGG | 0.524808 | 8.1:-52565373 | MS.gene36047:CDS |
| CGCTTTCGATGATGAAGATA+CGG | 0.525676 | 8.1:+52555993 | None:intergenic |
| CACTAGTTCTGTGCCTTGAA+AGG | 0.527319 | 8.1:-52573802 | MS.gene36047:CDS |
| TTTAAACCTACTTACACTGT+TGG | 0.530327 | 8.1:-52568363 | MS.gene36047:CDS |
| GGAGCTAAATTACTACTCTC+AGG | 0.536878 | 8.1:-52562474 | MS.gene36047:CDS |
| GCTGCAAGAAGCCGTAGGAA+TGG | 0.539955 | 8.1:-52556018 | MS.gene36047:CDS |
| AAACCACTAACCAGGGTAGT+TGG | 0.540219 | 8.1:+52574196 | None:intergenic |
| AAACGAAGAGGTGGCAGCTG+GGG | 0.541524 | 8.1:-52568971 | MS.gene36047:CDS |
| GAAACGAAGAGGTGGCAGCT+GGG | 0.546177 | 8.1:-52568972 | MS.gene36047:CDS |
| TCAATAGCATGTCTAGCAGC+AGG | 0.549937 | 8.1:+52562444 | None:intergenic |
| GCATGCTCTAGGAAACATCT+CGG | 0.550660 | 8.1:-52558185 | MS.gene36047:CDS |
| TGTAGTTTCAGTACTTGGGT+CGG | 0.550899 | 8.1:+52556396 | None:intergenic |
| AGCATCACAATGTTCATCAC+TGG | 0.550928 | 8.1:-52568734 | MS.gene36047:intron |
| ACTTGATAATGAGTGGAAGA+GGG | 0.552430 | 8.1:+52574044 | None:intergenic |
| AAGTGGAAGGCTACTATCCA+AGG | 0.552961 | 8.1:-52567289 | MS.gene36047:CDS |
| TACTTTATGAAACAGCATCC+AGG | 0.554953 | 8.1:-52558099 | MS.gene36047:CDS |
| TGAAGTAACTTGATAATGAG+TGG | 0.556956 | 8.1:+52574037 | None:intergenic |
| TTATAGAATGATAAGTGGAT+TGG | 0.557998 | 8.1:-52556479 | MS.gene36047:CDS |
| AATTGTCAAGATTCTCCAGA+AGG | 0.559293 | 8.1:+52571408 | None:intergenic |
| ACCGATTTCGCCAACTACCC+TGG | 0.569158 | 8.1:-52574206 | MS.gene36047:intron |
| AAAGGAGGTGATACAGACCT+TGG | 0.570033 | 8.1:-52568763 | MS.gene36047:CDS |
| AAGGAGGTGATACAGACCTT+GGG | 0.578352 | 8.1:-52568762 | MS.gene36047:CDS |
| ATCTTTCCATCTACCAAAGG+AGG | 0.591623 | 8.1:-52568778 | MS.gene36047:CDS |
| TGCTTGAAGGAGTTGTTGGG+AGG | 0.602619 | 8.1:+52574232 | None:intergenic |
| AGCTCAACCTATTGTGATGA+CGG | 0.603732 | 8.1:-52555964 | MS.gene36047:CDS |
| TTGATCGACGAGGACCAGAA+AGG | 0.603860 | 8.1:-52562413 | MS.gene36047:CDS |
| GAGCAATGATGATAAGTGGA+AGG | 0.604853 | 8.1:-52567302 | MS.gene36047:CDS |
| CATGCTCTAGGAAACATCTC+GGG | 0.609327 | 8.1:-52558184 | MS.gene36047:CDS |
| TTTGTGATCATAGCTATCAG+AGG | 0.612390 | 8.1:-52562495 | MS.gene36047:intron |
| ATTGTCAAGATTCTCCAGAA+GGG | 0.612890 | 8.1:+52571409 | None:intergenic |
| GAGCTCAAAGCTGACACCAT+CGG | 0.619703 | 8.1:-52558077 | MS.gene36047:intron |
| ATAATCTTTCCATCTACCAA+AGG | 0.634831 | 8.1:-52568781 | MS.gene36047:CDS |
| AAGATACGGACCCATTCCTA+CGG | 0.635459 | 8.1:+52556007 | None:intergenic |
| CAGCTAGCAGAGGTTGAGCA+TGG | 0.636743 | 8.1:-52567502 | MS.gene36047:CDS |
| CTAGATATAACTGCTGCATG+CGG | 0.647515 | 8.1:-52556232 | MS.gene36047:CDS |
| GAAATGGATGCAGCTAGCAG+AGG | 0.649349 | 8.1:-52567512 | MS.gene36047:intron |
| CGACGAGGACCAGAAAGGCA+TGG | 0.652455 | 8.1:-52562408 | MS.gene36047:CDS |
| TCACATATGCAGAAACGAAG+AGG | 0.661078 | 8.1:-52568983 | MS.gene36047:intron |
| ATTGTGATGCTATAATCCCA+AGG | 0.661427 | 8.1:+52568746 | None:intergenic |
| GTGCAGCTGCAAGAAGCCGT+AGG | 0.664105 | 8.1:-52556023 | MS.gene36047:intron |
| GAAAACACTTAAAGTTACAA+GGG | 0.679076 | 8.1:+52568313 | None:intergenic |
| CATATGCAGAAACGAAGAGG+TGG | 0.680999 | 8.1:-52568980 | MS.gene36047:intron |
| GCGATTGAGAAAATCTGTAG+CGG | 0.683960 | 8.1:+52574069 | None:intergenic |
| AGATTCAGAAATCCGTATAG+CGG | 0.690364 | 8.1:-52557076 | MS.gene36047:intron |
| TGATGACAAACCATGTACTG+TGG | 0.692128 | 8.1:+52573746 | None:intergenic |
| CATATCTCCATCAAGCACCA+CGG | 0.693939 | 8.1:+52556448 | None:intergenic |
| GTGGATTGGTGTCTCGACCG+TGG | 0.710959 | 8.1:-52556465 | MS.gene36047:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 52555950 | 52574267 | 52555950 | ID=MS.gene36047 |
| chr8.1 | mRNA | 52555950 | 52574267 | 52555950 | ID=MS.gene36047.t1;Parent=MS.gene36047 |
| chr8.1 | exon | 52574207 | 52574267 | 52574207 | ID=MS.gene36047.t1.exon1;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52574207 | 52574267 | 52574207 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52574046 | 52574109 | 52574046 | ID=MS.gene36047.t1.exon2;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52574046 | 52574109 | 52574046 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52573757 | 52573865 | 52573757 | ID=MS.gene36047.t1.exon3;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52573757 | 52573865 | 52573757 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52573601 | 52573663 | 52573601 | ID=MS.gene36047.t1.exon4;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52573601 | 52573663 | 52573601 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52571335 | 52571438 | 52571335 | ID=MS.gene36047.t1.exon5;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52571335 | 52571438 | 52571335 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52568927 | 52568993 | 52568927 | ID=MS.gene36047.t1.exon6;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52568927 | 52568993 | 52568927 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52568735 | 52568806 | 52568735 | ID=MS.gene36047.t1.exon7;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52568735 | 52568806 | 52568735 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52568298 | 52568459 | 52568298 | ID=MS.gene36047.t1.exon8;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52568298 | 52568459 | 52568298 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52567448 | 52567521 | 52567448 | ID=MS.gene36047.t1.exon9;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52567448 | 52567521 | 52567448 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52567259 | 52567356 | 52567259 | ID=MS.gene36047.t1.exon10;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52567259 | 52567356 | 52567259 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52565296 | 52565403 | 52565296 | ID=MS.gene36047.t1.exon11;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52565296 | 52565403 | 52565296 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52562398 | 52562504 | 52562398 | ID=MS.gene36047.t1.exon12;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52562398 | 52562504 | 52562398 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52558078 | 52558215 | 52558078 | ID=MS.gene36047.t1.exon13;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52558078 | 52558215 | 52558078 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52557077 | 52557124 | 52557077 | ID=MS.gene36047.t1.exon14;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52557077 | 52557124 | 52557077 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52556389 | 52556503 | 52556389 | ID=MS.gene36047.t1.exon15;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52556389 | 52556503 | 52556389 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52556163 | 52556263 | 52556163 | ID=MS.gene36047.t1.exon16;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52556163 | 52556263 | 52556163 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| chr8.1 | exon | 52555950 | 52556039 | 52555950 | ID=MS.gene36047.t1.exon17;Parent=MS.gene36047.t1 |
| chr8.1 | CDS | 52555950 | 52556039 | 52555950 | ID=cds.MS.gene36047.t1;Parent=MS.gene36047.t1 |
| Gene Sequence |
| Protein sequence |
