Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36197.t1 | XP_013448337.1 | 93.6 | 125 | 7 | 1 | 1 | 124 | 1 | 125 | 8.40E-62 | 246.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36197.t1 | A0A072TY19 | 93.6 | 125 | 7 | 1 | 1 | 124 | 1 | 125 | 6.1e-62 | 246.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene36197 | 0.80449 | 2.11E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene36197 | 0.80704 | 6.14E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36197.t1 | MTR_7g445390 | 97.600 | 125 | 2 | 1 | 1 | 124 | 1 | 125 | 4.68e-89 | 254 |
| MS.gene36197.t1 | MTR_7g445390 | 95.200 | 125 | 2 | 2 | 1 | 124 | 1 | 122 | 1.69e-84 | 242 |
| MS.gene36197.t1 | MTR_8g005880 | 59.677 | 124 | 33 | 4 | 1 | 124 | 1 | 107 | 1.91e-43 | 138 |
| MS.gene36197.t1 | MTR_5g065350 | 49.057 | 53 | 27 | 0 | 5 | 57 | 14 | 66 | 3.97e-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36197.t1 | AT3G60520 | 54.478 | 134 | 46 | 6 | 1 | 124 | 1 | 129 | 7.85e-35 | 117 |
| MS.gene36197.t1 | AT1G02070 | 54.615 | 130 | 49 | 4 | 5 | 124 | 2 | 131 | 2.51e-34 | 115 |
| MS.gene36197.t1 | AT3G60520 | 48.000 | 100 | 38 | 5 | 34 | 124 | 24 | 118 | 2.24e-15 | 67.4 |
Find 36 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCAACCGATCGGAATATT+CGG | 0.326610 | 7.1:+55547573 | MS.gene36197:CDS |
| AGGTATTGTAGCAACTACTA+TGG | 0.356994 | 7.1:+55547439 | MS.gene36197:intron |
| GGTCACCGGTTTCTTTGAAT+TGG | 0.367398 | 7.1:-55547530 | None:intergenic |
| TGTCGCGACGATCTCATTTC+CGG | 0.389983 | 7.1:-55547551 | None:intergenic |
| TTGCAACGGAAAAGCTTGTC+AGG | 0.430390 | 7.1:-55546961 | None:intergenic |
| CGGAGATAGAACAGTGTGAT+TGG | 0.433454 | 7.1:+55547470 | MS.gene36197:CDS |
| ACCAACCGATCGGAATATTC+GGG | 0.471379 | 7.1:+55547574 | MS.gene36197:CDS |
| TGGCACCAATTCAAAGAAAC+CGG | 0.486182 | 7.1:+55547525 | MS.gene36197:CDS |
| AAAGTAATACATACGAGTGT+TGG | 0.494678 | 7.1:-55547002 | None:intergenic |
| ATCGCCACACATACAACAAA+CGG | 0.501960 | 7.1:-55546929 | None:intergenic |
| GCGGTCCGTGGCGACGGCGA+AGG | 0.502361 | 7.1:-55547655 | None:intergenic |
| CGTGTGGCGGTCCGTGGCGA+CGG | 0.515666 | 7.1:-55547661 | None:intergenic |
| ACGATCTCATTTCCGGTCAC+CGG | 0.516236 | 7.1:-55547544 | None:intergenic |
| TGTTGTATGTGTGGCGATGT+TGG | 0.520379 | 7.1:+55546934 | MS.gene36197:CDS |
| GAAGAGGGTGGCTCTGAGAA+GGG | 0.527687 | 7.1:+55547625 | MS.gene36197:CDS |
| TGAAGAGGGTGGCTCTGAGA+AGG | 0.532977 | 7.1:+55547624 | MS.gene36197:CDS |
| ATACATACGAGTGTTGGAAT+CGG | 0.550310 | 7.1:-55546996 | None:intergenic |
| AGAGTCCTTCGCCGTCGCCA+CGG | 0.553436 | 7.1:+55547650 | MS.gene36197:CDS |
| TACCTACGTGTGGCGGTCCG+TGG | 0.554233 | 7.1:-55547667 | None:intergenic |
| ACCCGAATATTCCGATCGGT+TGG | 0.554818 | 7.1:-55547575 | None:intergenic |
| AATTCAAAGAAACCGGTGAC+CGG | 0.559840 | 7.1:+55547532 | MS.gene36197:CDS |
| AAACAACATGATCGTGAAGA+GGG | 0.586473 | 7.1:+55547610 | MS.gene36197:CDS |
| ACGAGTGTTGGAATCGGTGG+CGG | 0.586739 | 7.1:-55546990 | None:intergenic |
| GGTGGCGGCATTTGTTGCAA+CGG | 0.592229 | 7.1:-55546975 | None:intergenic |
| AAGAGGGTGGCTCTGAGAAG+GGG | 0.612751 | 7.1:+55547626 | MS.gene36197:CDS |
| TAAACAACATGATCGTGAAG+AGG | 0.621485 | 7.1:+55547609 | MS.gene36197:CDS |
| GAGAAGCTTGTACCTACGTG+TGG | 0.625438 | 7.1:-55547677 | None:intergenic |
| CATACGAGTGTTGGAATCGG+TGG | 0.628121 | 7.1:-55546993 | None:intergenic |
| TCTCACCCGAATATTCCGAT+CGG | 0.641982 | 7.1:-55547579 | None:intergenic |
| CGTCGCGACAACCAACCGAT+CGG | 0.647059 | 7.1:+55547564 | MS.gene36197:CDS |
| ACGTAGGTACAAGCTTCTCA+AGG | 0.654964 | 7.1:+55547681 | MS.gene36197:CDS |
| AAGCTTGTACCTACGTGTGG+CGG | 0.656745 | 7.1:-55547674 | None:intergenic |
| CAAACCGTTTGTTGTATGTG+TGG | 0.658560 | 7.1:+55546925 | MS.gene36197:CDS |
| CAACATGATCGTGAAGAGGG+TGG | 0.664857 | 7.1:+55547613 | MS.gene36197:CDS |
| CAACTACTATGGAGAACTAG+CGG | 0.694469 | 7.1:+55547450 | MS.gene36197:CDS |
| CGCCACGGACCGCCACACGT+AGG | 0.759309 | 7.1:+55547665 | MS.gene36197:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATTGTTATTATTTTATT+AGG | + | chr7.1:55547370-55547389 | MS.gene36197:intron | 10.0% |
| !!! | ATTTTAATGTTGTTTTTTTA+AGG | + | chr7.1:55547419-55547438 | MS.gene36197:intron | 10.0% |
| !!! | CAACATTAAAATAAATTTTA+AGG | - | chr7.1:55547411-55547430 | None:intergenic | 10.0% |
| !!! | CAAGTATATTATTTTTTGTT+TGG | + | chr7.1:55547228-55547247 | MS.gene36197:intron | 15.0% |
| !!! | TCATCATCTTTTATTTAATT+GGG | + | chr7.1:55547089-55547108 | MS.gene36197:intron | 15.0% |
| !!! | TTCATCATCTTTTATTTAAT+TGG | + | chr7.1:55547088-55547107 | MS.gene36197:intron | 15.0% |
| !! | AACATGACTTTATAACTTTA+AGG | - | chr7.1:55547195-55547214 | None:intergenic | 20.0% |
| !! | AACCATAGTATAAAACATTA+GGG | + | chr7.1:55547254-55547273 | MS.gene36197:intron | 20.0% |
| !! | TAACCATAGTATAAAACATT+AGG | + | chr7.1:55547253-55547272 | MS.gene36197:intron | 20.0% |
| !!! | ATCATCTTTTATTTAATTGG+GGG | + | chr7.1:55547091-55547110 | MS.gene36197:intron | 20.0% |
| !!! | CATCATCTTTTATTTAATTG+GGG | + | chr7.1:55547090-55547109 | MS.gene36197:intron | 20.0% |
| ! | GAGAAAAATTCAAGACATAT+TGG | + | chr7.1:55547505-55547524 | MS.gene36197:CDS | 25.0% |
| !! | TTGAATTTTTCTCTTCACTT+TGG | - | chr7.1:55547498-55547517 | None:intergenic | 25.0% |
| AAAGTAATACATACGAGTGT+TGG | - | chr7.1:55547005-55547024 | None:intergenic | 30.0% | |
| ! | GACCCTAATGTTTTATACTA+TGG | - | chr7.1:55547259-55547278 | None:intergenic | 30.0% |
| !! | ATCTTTTATTTAATTGGGGG+TGG | + | chr7.1:55547094-55547113 | MS.gene36197:intron | 30.0% |
| AAACAACATGATCGTGAAGA+GGG | + | chr7.1:55547610-55547629 | MS.gene36197:CDS | 35.0% | |
| TAAACAACATGATCGTGAAG+AGG | + | chr7.1:55547609-55547628 | MS.gene36197:CDS | 35.0% | |
| ! | AGGTATTGTAGCAACTACTA+TGG | + | chr7.1:55547439-55547458 | MS.gene36197:intron | 35.0% |
| !! | ATACATACGAGTGTTGGAAT+CGG | - | chr7.1:55546999-55547018 | None:intergenic | 35.0% |
| AACCAACCGATCGGAATATT+CGG | + | chr7.1:55547573-55547592 | MS.gene36197:CDS | 40.0% | |
| AATTCAAAGAAACCGGTGAC+CGG | + | chr7.1:55547532-55547551 | MS.gene36197:CDS | 40.0% | |
| ATCGCCACACATACAACAAA+CGG | - | chr7.1:55546932-55546951 | None:intergenic | 40.0% | |
| CAACTACTATGGAGAACTAG+CGG | + | chr7.1:55547450-55547469 | MS.gene36197:CDS | 40.0% | |
| TGGCACCAATTCAAAGAAAC+CGG | + | chr7.1:55547525-55547544 | MS.gene36197:CDS | 40.0% | |
| !! | CAAACCGTTTGTTGTATGTG+TGG | + | chr7.1:55546925-55546944 | MS.gene36197:CDS | 40.0% |
| ACCAACCGATCGGAATATTC+GGG | + | chr7.1:55547574-55547593 | MS.gene36197:CDS | 45.0% | |
| ACGTAGGTACAAGCTTCTCA+AGG | + | chr7.1:55547681-55547700 | MS.gene36197:CDS | 45.0% | |
| CGGAGATAGAACAGTGTGAT+TGG | + | chr7.1:55547470-55547489 | MS.gene36197:CDS | 45.0% | |
| TCTCACCCGAATATTCCGAT+CGG | - | chr7.1:55547582-55547601 | None:intergenic | 45.0% | |
| TGTTGTATGTGTGGCGATGT+TGG | + | chr7.1:55546934-55546953 | MS.gene36197:CDS | 45.0% | |
| TTGCAACGGAAAAGCTTGTC+AGG | - | chr7.1:55546964-55546983 | None:intergenic | 45.0% | |
| !! | GGTCACCGGTTTCTTTGAAT+TGG | - | chr7.1:55547533-55547552 | None:intergenic | 45.0% |
| AAGCTTGTACCTACGTGTGG+CGG | - | chr7.1:55547677-55547696 | None:intergenic | 50.0% | |
| ACCCGAATATTCCGATCGGT+TGG | - | chr7.1:55547578-55547597 | None:intergenic | 50.0% | |
| ACGATCTCATTTCCGGTCAC+CGG | - | chr7.1:55547547-55547566 | None:intergenic | 50.0% | |
| CAACATGATCGTGAAGAGGG+TGG | + | chr7.1:55547613-55547632 | MS.gene36197:CDS | 50.0% | |
| GAGAAGCTTGTACCTACGTG+TGG | - | chr7.1:55547680-55547699 | None:intergenic | 50.0% | |
| TGTCGCGACGATCTCATTTC+CGG | - | chr7.1:55547554-55547573 | None:intergenic | 50.0% | |
| !! | CATACGAGTGTTGGAATCGG+TGG | - | chr7.1:55546996-55547015 | None:intergenic | 50.0% |
| ! | GGTGGCGGCATTTGTTGCAA+CGG | - | chr7.1:55546978-55546997 | None:intergenic | 55.0% |
| !! | AAGAGGGTGGCTCTGAGAAG+GGG | + | chr7.1:55547626-55547645 | MS.gene36197:CDS | 55.0% |
| !! | ACGAGTGTTGGAATCGGTGG+CGG | - | chr7.1:55546993-55547012 | None:intergenic | 55.0% |
| !! | GAAGAGGGTGGCTCTGAGAA+GGG | + | chr7.1:55547625-55547644 | MS.gene36197:CDS | 55.0% |
| !! | TGAAGAGGGTGGCTCTGAGA+AGG | + | chr7.1:55547624-55547643 | MS.gene36197:CDS | 55.0% |
| CGTCGCGACAACCAACCGAT+CGG | + | chr7.1:55547564-55547583 | MS.gene36197:CDS | 60.0% | |
| AGAGTCCTTCGCCGTCGCCA+CGG | + | chr7.1:55547650-55547669 | MS.gene36197:CDS | 65.0% | |
| TACCTACGTGTGGCGGTCCG+TGG | - | chr7.1:55547670-55547689 | None:intergenic | 65.0% | |
| CGCCACGGACCGCCACACGT+AGG | + | chr7.1:55547665-55547684 | MS.gene36197:CDS | 75.0% | |
| CGTGTGGCGGTCCGTGGCGA+CGG | - | chr7.1:55547664-55547683 | None:intergenic | 75.0% | |
| ! | GCGGTCCGTGGCGACGGCGA+AGG | - | chr7.1:55547658-55547677 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 55546913 | 55547717 | 55546913 | ID=MS.gene36197 |
| chr7.1 | mRNA | 55546913 | 55547717 | 55546913 | ID=MS.gene36197.t1;Parent=MS.gene36197 |
| chr7.1 | exon | 55546913 | 55547010 | 55546913 | ID=MS.gene36197.t1.exon1;Parent=MS.gene36197.t1 |
| chr7.1 | CDS | 55546913 | 55547010 | 55546913 | ID=cds.MS.gene36197.t1;Parent=MS.gene36197.t1 |
| chr7.1 | exon | 55547441 | 55547717 | 55547441 | ID=MS.gene36197.t1.exon2;Parent=MS.gene36197.t1 |
| chr7.1 | CDS | 55547441 | 55547717 | 55547441 | ID=cds.MS.gene36197.t1;Parent=MS.gene36197.t1 |
| Gene Sequence |
| Protein sequence |
