AlfalfaGEDB | MS.gene36314

M. sativa cultivar XinJiangDaYe / MS.gene36314

KEGG: K10418
GO: GO:0005575 GO:0005875 GO:0007017 GO:0008150 GO:0009987 GO:0030286 GO:0032991 GO:1902494
PFAM: PF01221.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36314.t1	XP_003597748.1	100	91	0	0	1	91	1	91	2.00E-44	188

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36314.t1	Q94748	47.2	89	45	1	1	89	1	87	4.1e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36314.t1	G7II18	100.0	91	0	0	1	91	1	91	1.4e-44	188.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene061266	MS.gene36314	0.814464	1.52E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36314.t1	MTR_2g101850	100.000	91	0	0	1	91	1	91	7.67e-65	190
MS.gene36314.t1	MTR_6g087770	75.556	90	22	0	1	90	1	90	6.48e-50	152
MS.gene36314.t1	MTR_8g464690	54.444	90	41	0	1	90	1	90	6.83e-29	100
MS.gene36314.t1	MTR_4g114060	49.367	79	40	0	11	89	159	237	5.05e-22	86.3
MS.gene36314.t1	MTR_4g107350	46.067	89	46	2	2	89	213	300	1.20e-20	83.6
MS.gene36314.t1	MTR_8g104900	41.667	84	47	1	8	89	41	124	2.57e-18	73.9
MS.gene36314.t1	MTR_1g031880	41.250	80	45	1	12	89	59	138	6.76e-18	73.2
MS.gene36314.t1	MTR_4g120150	40.698	86	49	1	5	90	46	129	3.30e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36314.t1	AT1G52245	84.444	90	14	0	1	90	1	90	2.00e-55	166
MS.gene36314.t1	AT3G16120	78.889	90	19	0	1	90	1	90	4.72e-50	153
MS.gene36314.t1	AT3G16120	78.889	90	19	0	1	90	1	90	4.72e-50	153
MS.gene36314.t1	AT4G27360	54.444	90	41	0	1	90	1	90	1.35e-30	104
MS.gene36314.t1	AT5G20110	50.000	78	39	0	12	89	123	200	4.26e-20	80.5
MS.gene36314.t1	AT4G15930	42.353	85	47	1	5	89	39	121	1.04e-18	74.7
MS.gene36314.t1	AT1G23220	38.636	88	53	1	3	89	33	120	3.39e-18	73.6
MS.gene36314.t1	AT4G15930	44.262	61	32	1	5	65	39	97	6.59e-11	54.3

Find 12 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCAAACTCCTTTGAAAATA+AGG	0.252205	2.2:-1905213	None:intergenic
CAGGAGTTTGATGCAAGATA+TGG	0.332591	2.2:+1905094	MS.gene36314:intron
TGTGTGGTAGGTTCAAGTTT+TGG	0.339581	2.2:+1905130	MS.gene36314:CDS
GCTTGAATCTGCATCTTAGT+TGG	0.432610	2.2:-1904819	None:intergenic
GGTGGATGGCAATGTGTGGT+AGG	0.511909	2.2:+1905118	MS.gene36314:CDS
TAAGATGCAGATTCAAGCCA+TGG	0.595332	2.2:+1904824	MS.gene36314:CDS
GTAACTTCAAAGATGTTGGA+AGG	0.606545	2.2:+1904768	None:intergenic
AGCTTCAGAAGCATAAGCCA+TGG	0.620640	2.2:-1904841	None:intergenic
TTTGATGCAAGATATGGTGG+TGG	0.642657	2.2:+1905100	MS.gene36314:CDS
TGGTGGTGGATGGCAATGTG+TGG	0.654380	2.2:+1905114	MS.gene36314:CDS
ATGCAAGATATGGTGGTGGA+TGG	0.656816	2.2:+1905104	MS.gene36314:CDS
GAGTTTGATGCAAGATATGG+TGG	0.685518	2.2:+1905097	MS.gene36314:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTCAATTTCCTTATTTTCAA+AGG	+	chr2.2:1905205-1905224	MS.gene36314:CDS	25.0%
!!!	TGTTTTTTCACTCATTCTAA+GGG	+	chr2.2:1905154-1905173	MS.gene36314:CDS	25.0%
!!!	TTTTCTTTGAATGTCATTTC+AGG	+	chr2.2:1905075-1905094	MS.gene36314:intron	25.0%
!!!	GTGTTTTTTCACTCATTCTA+AGG	+	chr2.2:1905153-1905172	MS.gene36314:CDS	30.0%
	CATTGCTGCTCACATAAAAA+AGG	+	chr2.2:1904896-1904915	MS.gene36314:CDS	35.0%
!!!	CTTTTTTATGTGAGCAGCAA+TGG	-	chr2.2:1904898-1904917	None:intergenic	35.0%
	CAGGAGTTTGATGCAAGATA+TGG	+	chr2.2:1905094-1905113	MS.gene36314:intron	40.0%
	GAGTTTGATGCAAGATATGG+TGG	+	chr2.2:1905097-1905116	MS.gene36314:CDS	40.0%
	GCTTGAATCTGCATCTTAGT+TGG	-	chr2.2:1904822-1904841	None:intergenic	40.0%
	TAAGATGCAGATTCAAGCCA+TGG	+	chr2.2:1904824-1904843	MS.gene36314:CDS	40.0%
	TTTGATGCAAGATATGGTGG+TGG	+	chr2.2:1905100-1905119	MS.gene36314:CDS	40.0%
!!	GTGTGGTAGGTTCAAGTTTT+GGG	+	chr2.2:1905131-1905150	MS.gene36314:CDS	40.0%
!!	TGTGTGGTAGGTTCAAGTTT+TGG	+	chr2.2:1905130-1905149	MS.gene36314:CDS	40.0%
!	AGCTTCAGAAGCATAAGCCA+TGG	-	chr2.2:1904844-1904863	None:intergenic	45.0%
!!	ATGCAAGATATGGTGGTGGA+TGG	+	chr2.2:1905104-1905123	MS.gene36314:CDS	45.0%
	GGTGGATGGCAATGTGTGGT+AGG	+	chr2.2:1905118-1905137	MS.gene36314:CDS	55.0%
!!	TGGTGGTGGATGGCAATGTG+TGG	+	chr2.2:1905114-1905133	MS.gene36314:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	1904780	1905234	1904780	ID=MS.gene36314
chr2.2	mRNA	1904780	1905234	1904780	ID=MS.gene36314.t1;Parent=MS.gene36314
chr2.2	exon	1904780	1904917	1904780	ID=MS.gene36314.t1.exon1;Parent=MS.gene36314.t1
chr2.2	CDS	1904780	1904917	1904780	ID=cds.MS.gene36314.t1;Parent=MS.gene36314.t1
chr2.2	exon	1905097	1905234	1905097	ID=MS.gene36314.t1.exon2;Parent=MS.gene36314.t1
chr2.2	CDS	1905097	1905234	1905097	ID=cds.MS.gene36314.t1;Parent=MS.gene36314.t1

Gene Sequence

>MS.gene36314

ATGTTGGAAGGTAAAGCTATGATAGAAGATACTGACATGCCAACTAAGATGCAGATTCAAGCCATGGCTTATGCTTCTGAAGCTCTTGATCTATATGATGTTATTGATTGCAAATCCATTGCTGCTCACATAAAAAAGGTTTACATTTTTCTCTTCAATCATTGTATTATACACACAACACAACATCATTAAGTTGTTCATTTATAGATGTATAGTAATCACTTATTTATTAAGAAACTAAATAACTGACTAAACATGTATTATAAAGATAAATTGATATTCAAATATTATTTATTTTTCTTTGAATGTCATTTCAGGAGTTTGATGCAAGATATGGTGGTGGATGGCAATGTGTGGTAGGTTCAAGTTTTGGGTGTTTTTTCACTCATTCTAAGGGAACTTTCATATACTTCACTCTTGAGACTCTCAATTTCCTTATTTTCAAAGGAGTTTGA

Protein sequence

>MS.gene36314.t1

MLEGKAMIEDTDMPTKMQIQAMAYASEALDLYDVIDCKSIAAHIKKEFDARYGGGWQCVVGSSFGCFFTHSKGTFIYFTLETLNFLIFKGV