AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36333

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36333.t1 MTR_2g101520 99.392 329 2 0 1 329 1 329 0.0 679
MS.gene36333.t1 MTR_6g488100 47.688 346 127 11 1 329 14 322 2.18e-89 270
MS.gene36333.t1 MTR_6g488100 47.293 351 126 12 1 329 14 327 5.11e-88 267
MS.gene36333.t1 MTR_8g103065 48.148 351 130 12 1 329 15 335 7.78e-79 244
MS.gene36333.t1 MTR_6g488100 43.902 287 110 10 1 270 14 266 8.84e-60 193
MS.gene36333.t1 MTR_6g488100 44.061 261 97 9 1 244 14 242 4.25e-53 176
MS.gene36333.t1 MTR_8g103065 44.086 279 104 12 1 257 15 263 2.29e-44 153
MS.gene36333.t1 MTR_2g081820 48.026 152 48 3 175 326 312 432 4.92e-35 132
MS.gene36333.t1 MTR_2g081820 48.026 152 48 3 175 326 313 433 5.42e-35 132
MS.gene36333.t1 MTR_2g011070 46.250 160 54 3 167 326 283 410 1.04e-32 125
MS.gene36333.t1 MTR_8g466800 47.097 155 51 3 175 329 320 443 6.77e-32 124
MS.gene36333.t1 MTR_1g107185 45.695 151 60 3 175 324 138 267 6.91e-32 120
MS.gene36333.t1 MTR_3g112170 46.099 141 46 4 188 326 339 451 1.57e-30 120
MS.gene36333.t1 MTR_1g094085 42.857 154 54 4 175 325 405 527 1.05e-26 110
MS.gene36333.t1 MTR_4g087300 44.444 135 35 2 189 323 33 127 2.10e-26 102
MS.gene36333.t1 MTR_4g087300 44.444 135 35 2 189 323 56 150 2.16e-26 102
MS.gene36333.t1 MTR_4g087300 44.444 135 33 3 189 323 56 148 1.77e-24 97.8
MS.gene36333.t1 MTR_8g466800 64.286 70 25 0 175 244 320 389 5.95e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36333.t1 AT4G29100 42.145 401 151 15 1 329 15 406 7.83e-71 226
MS.gene36333.t1 AT2G20100 59.887 177 57 4 163 329 189 361 8.28e-61 198
MS.gene36333.t1 AT2G20100 47.534 223 57 5 163 329 189 407 1.26e-51 176
MS.gene36333.t1 AT4G29100 38.462 338 131 13 1 270 15 343 9.96e-43 151
MS.gene36333.t1 AT2G20100 64.035 114 31 3 163 270 189 298 1.91e-36 134
MS.gene36333.t1 AT2G20100 64.035 114 31 3 163 270 189 298 1.91e-36 134
MS.gene36333.t1 AT1G27660 48.503 167 45 6 173 328 316 452 1.79e-34 131
MS.gene36333.t1 AT3G19500 48.667 150 44 3 175 324 140 256 2.79e-33 124
MS.gene36333.t1 AT3G20640 44.516 155 54 3 175 329 269 391 2.44e-31 121
MS.gene36333.t1 AT3G20640 44.516 155 54 3 175 329 331 453 5.24e-31 122
MS.gene36333.t1 AT1G61660 44.079 152 56 2 175 326 263 385 2.27e-28 113
MS.gene36333.t1 AT1G61660 44.079 152 56 2 175 326 266 388 2.41e-28 113
MS.gene36333.t1 AT1G61660 44.079 152 56 2 175 326 266 388 2.41e-28 113
MS.gene36333.t1 AT2G31730 40.441 136 41 1 189 324 39 134 3.94e-26 102
MS.gene36333.t1 AT2G31730 40.441 136 41 1 189 324 39 134 3.94e-26 102
MS.gene36333.t1 AT4G05170 44.776 134 50 3 175 306 41 152 9.66e-26 101
MS.gene36333.t1 AT1G31050 41.718 163 64 5 165 325 299 432 4.19e-25 105
MS.gene36333.t1 AT1G31050 41.718 163 64 5 165 325 295 428 4.42e-25 105
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 29 124 4.50e-25 99.0
MS.gene36333.t1 AT4G05170 44.776 134 50 3 175 306 134 245 4.65e-25 102
MS.gene36333.t1 AT1G31050 41.718 163 64 5 165 325 295 428 4.69e-25 105
MS.gene36333.t1 AT4G05170 44.776 134 50 3 175 306 159 270 5.53e-25 102
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT1G05710 39.706 136 42 1 189 324 37 132 5.65e-25 99.0
MS.gene36333.t1 AT4G05170 44.776 134 50 3 175 306 177 288 5.76e-25 103
MS.gene36333.t1 AT4G21340 48.175 137 43 5 175 309 176 286 2.15e-23 98.6
MS.gene36333.t1 AT1G05710 42.647 136 60 4 189 324 29 146 5.15e-23 94.0
MS.gene36333.t1 AT1G05710 42.647 136 60 4 189 324 29 146 5.15e-23 94.0
MS.gene36333.t1 AT1G05710 42.647 136 60 4 189 324 37 154 7.51e-23 94.0
MS.gene36333.t1 AT1G05710 42.647 136 60 4 189 324 37 154 7.51e-23 94.0
MS.gene36333.t1 AT1G05710 42.647 136 60 4 189 324 37 154 7.51e-23 94.0
MS.gene36333.t1 AT1G05710 42.647 136 60 4 189 324 37 154 7.51e-23 94.0
MS.gene36333.t1 AT4G05170 64.789 71 25 0 175 245 159 229 8.77e-22 92.8
MS.gene36333.t1 AT3G19500 65.152 66 23 0 175 240 140 205 7.62e-21 89.7
MS.gene36333.t1 AT1G61660 64.789 71 25 0 175 245 266 336 1.29e-20 91.3
MS.gene36333.t1 AT1G61660 64.789 71 25 0 175 245 266 336 1.29e-20 91.3
MS.gene36333.t1 AT1G49830 40.945 127 41 3 199 325 133 225 3.18e-20 88.6
MS.gene36333.t1 AT1G49830 40.945 127 41 3 199 325 134 226 3.21e-20 88.6
MS.gene36333.t1 AT3G20640 61.972 71 26 1 175 245 331 400 5.41e-20 90.5
MS.gene36333.t1 AT4G05170 70.690 58 17 0 175 232 159 216 1.44e-19 86.3
MS.gene36333.t1 AT4G05170 70.690 58 17 0 175 232 159 216 1.44e-19 86.3
MS.gene36333.t1 AT3G20640 63.793 58 20 1 175 232 331 387 2.68e-15 76.6
MS.gene36333.t1 AT1G61660 34.641 153 69 3 174 326 245 366 3.23e-14 73.2
MS.gene36333.t1 AT1G31050 52.857 70 32 1 166 234 296 365 2.08e-13 70.9
MS.gene36333.t1 AT1G31050 52.857 70 32 1 166 234 300 369 2.22e-13 70.9

Find 83 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCAAGAGTTCCATTTA+TGG 0.161942 2.2:-2088488 MS.gene36333:CDS
TGCTTTATTATATCCATAAA+TGG 0.195130 2.2:+2088475 None:intergenic
CAACATGTTGGAAGTGAAAA+TGG 0.269929 2.2:-2086085 MS.gene36333:CDS
TGTGAGTAATTAACCTTTAG+AGG 0.282892 2.2:+2088031 None:intergenic
GTGAAAATGGAGCTGATTAC+TGG 0.289834 2.2:-2086072 MS.gene36333:CDS
CTTAAGTAGTGATAATATAT+TGG 0.313482 2.2:-2088317 MS.gene36333:CDS
CATACCTGACCGGGGTCTTC+AGG 0.315826 2.2:+2086973 None:intergenic
AAGAATACCTGGTTTGGAAT+TGG 0.331532 2.2:+2086636 None:intergenic
CACCAGCTAGTTTCTCCATT+TGG 0.331535 2.2:-2087716 MS.gene36333:CDS
ACAGAATGCAGAAGGTAATA+AGG 0.336565 2.2:-2086158 MS.gene36333:intron
GATTACTGGGCACCAGCATT+TGG 0.361562 2.2:-2086058 MS.gene36333:CDS
TGGTTCATCTTGGTCACATA+TGG 0.375811 2.2:-2088374 MS.gene36333:CDS
CAGATATTTGATCTGGTAGT+TGG 0.375958 2.2:+2088260 None:intergenic
GGATGCATGCTCCACCAGTT+AGG 0.382855 2.2:+2088959 None:intergenic
AGCTAGTGATATGAGAGCTT+GGG 0.388897 2.2:+2088338 None:intergenic
TGATGGCTGGGAACCCTAAC+TGG 0.390562 2.2:-2088973 MS.gene36333:CDS
GGCACTCAGCACCCCTTACT+TGG 0.395598 2.2:-2087227 MS.gene36333:CDS
CTGGATCACTTGGTTCATCT+TGG 0.397211 2.2:-2088384 MS.gene36333:CDS
GATGCTGCAGTGTCCAAGTA+AGG 0.413970 2.2:+2087214 None:intergenic
CATGCTAACTCTGGATCACT+TGG 0.416683 2.2:-2088394 MS.gene36333:CDS
TGAGAGCTTGGGGAAGAAAC+TGG 0.431844 2.2:+2088349 None:intergenic
TAGAAGCCATTGGATACATC+AGG 0.435008 2.2:-2087447 MS.gene36333:CDS
TTTACCTCAGATATTTGATC+TGG 0.452488 2.2:+2088253 None:intergenic
CCAATAAAGCATACCTGACC+GGG 0.469066 2.2:+2086964 None:intergenic
AAGCTAGTGATATGAGAGCT+TGG 0.469144 2.2:+2088337 None:intergenic
ATAAATGGAACTCTTGAAGA+TGG 0.469340 2.2:+2088490 None:intergenic
TAGTTTGTTACCTTTCCAAA+TGG 0.469992 2.2:+2087701 None:intergenic
CTGAAGTACCTCCTCAATCA+TGG 0.473133 2.2:-2088859 MS.gene36333:CDS
TCTGTATTATTAGAAGCCAT+TGG 0.473376 2.2:-2087457 MS.gene36333:CDS
CTGGAGTTAATAAGAAGGCT+AGG 0.474420 2.2:-2088078 MS.gene36333:CDS
GCAAAGGACTTGAAGAGTAG+AGG 0.478728 2.2:-2086136 MS.gene36333:CDS
ATCCCTCCTCAATATATGCT+TGG 0.481586 2.2:-2088923 MS.gene36333:CDS
TCCAATAAAGCATACCTGAC+CGG 0.484473 2.2:+2086963 None:intergenic
CTCAAGCCAACCACCTCTAA+AGG 0.491796 2.2:-2088044 MS.gene36333:intron
GATGCATGCTCCACCAGTTA+GGG 0.504766 2.2:+2088960 None:intergenic
CCATTGTAGCAAATGATGGC+TGG 0.505228 2.2:-2088986 None:intergenic
TGGGCACCAGCATTTGGAAG+TGG 0.511663 2.2:-2086052 MS.gene36333:CDS
GGAGGGATATTCAAAGATGA+TGG 0.512004 2.2:+2088938 None:intergenic
AGAGGTGGTTGGCTTGAGGA+TGG 0.512760 2.2:+2088049 None:intergenic
TAGTGTAATAGCTCAACTAC+TGG 0.514825 2.2:-2088097 MS.gene36333:CDS
CCAAGTCTCTCTTCTTCACC+AGG 0.522523 2.2:+2088568 None:intergenic
GAGGAGTTTCATGCTAACTC+TGG 0.527314 2.2:-2088403 MS.gene36333:CDS
ATTTCCTGAAGACCCCGGTC+AGG 0.529122 2.2:-2086977 MS.gene36333:intron
TAAAACCCACTTCCAAATGC+TGG 0.531421 2.2:+2086046 None:intergenic
AATTAACCTTTAGAGGTGGT+TGG 0.534058 2.2:+2088038 None:intergenic
CTTTAGAGGTGGTTGGCTTG+AGG 0.534870 2.2:+2088045 None:intergenic
TTTCTGTCAAGGAATTGAAA+GGG 0.538293 2.2:+2088888 None:intergenic
GTTTCTACAGAGTCAAATTG+AGG 0.544344 2.2:-2087425 MS.gene36333:intron
CAAAGGACTTGAAGAGTAGA+GGG 0.545063 2.2:-2086135 MS.gene36333:CDS
TTCCAAATGGAGAAACTAGC+TGG 0.547701 2.2:+2087714 None:intergenic
CATTGTAGCAAATGATGGCT+GGG 0.551321 2.2:-2088985 None:intergenic
GCTAGTTTCTCCATTTGGAA+AGG 0.554411 2.2:-2087711 MS.gene36333:intron
CCTGGTGAAGAAGAGAGACT+TGG 0.557044 2.2:-2088568 MS.gene36333:CDS
GGGCACCAGCATTTGGAAGT+GGG 0.558978 2.2:-2086051 MS.gene36333:CDS
TGAAAATGGAGCTGATTACT+GGG 0.559536 2.2:-2086071 MS.gene36333:CDS
TATAATAAACAGAATGCAGA+AGG 0.559856 2.2:-2086166 MS.gene36333:intron
AGTAGTTGACTCCATGATTG+AGG 0.561864 2.2:+2088848 None:intergenic
TGGAGTTAATAAGAAGGCTA+GGG 0.563568 2.2:-2088077 MS.gene36333:CDS
GAAGAGTAGAGGGTTGTGCT+TGG 0.563799 2.2:-2086125 MS.gene36333:CDS
ACATGTTGTGTGCATGACAC+TGG 0.568881 2.2:+2086100 None:intergenic
AACTACTGGAGTTAATAAGA+AGG 0.572553 2.2:-2088083 MS.gene36333:CDS
TGCAGAAGGTAATAAGGCAA+AGG 0.582379 2.2:-2086152 MS.gene36333:CDS
ATCTAGCTGTTGGATGACAC+TGG 0.583620 2.2:-2086678 MS.gene36333:intron
GAATCTGAATCAGGTGAGGA+AGG 0.588844 2.2:-2087768 MS.gene36333:intron
GAAGATCCAAGCATATATTG+AGG 0.591487 2.2:+2088917 None:intergenic
TAGAAACCTGATGTATCCAA+TGG 0.592817 2.2:+2087441 None:intergenic
ATGCTGCAGTGTCCAAGTAA+GGG 0.597172 2.2:+2087215 None:intergenic
ATAAAGCATGAAGTTTCTCA+AGG 0.600376 2.2:-2088460 MS.gene36333:CDS
GAGCGCTCCAATTCCAAACC+AGG 0.604751 2.2:-2086643 MS.gene36333:intron
GCTAGTGATATGAGAGCTTG+GGG 0.616346 2.2:+2088339 None:intergenic
GATCCAAGCATATATTGAGG+AGG 0.624438 2.2:+2088920 None:intergenic
ATCCAAGCATATATTGAGGA+GGG 0.625532 2.2:+2088921 None:intergenic
CAGGTGAGGAAGGAGAAGCT+AGG 0.634871 2.2:-2087758 MS.gene36333:CDS
TCTGTATTTCCTGAAGACCC+CGG 0.639899 2.2:-2086982 MS.gene36333:CDS
GAGTAATTAACCTTTAGAGG+TGG 0.639994 2.2:+2088034 None:intergenic
GTGTCATGCACACAACATGT+TGG 0.662430 2.2:-2086097 MS.gene36333:CDS
TGCTGCAGTGTCCAAGTAAG+GGG 0.663325 2.2:+2087216 None:intergenic
TCAACAAGGGCATCATCATG+AGG 0.663625 2.2:-2088422 MS.gene36333:CDS
ACTACCAGATCAAATATCTG+AGG 0.678408 2.2:-2088257 MS.gene36333:intron
AGTTGACTCCATGATTGAGG+AGG 0.689840 2.2:+2088851 None:intergenic
ATTGGATTTCACTTACAACA+AGG 0.702031 2.2:-2088299 MS.gene36333:CDS
TGGCTGGGAACCCTAACTGG+TGG 0.716766 2.2:-2088970 MS.gene36333:CDS
CAATAAAGCATACCTGACCG+GGG 0.742945 2.2:+2086965 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG + chr2.2:2088496-2088477 None:intergenic 0.0%
!! TTATAAAATAAAATTAAAAA+AGG + chr2.2:2087985-2088004 None:intergenic 0.0%
!! AAAATAAAATTAAAAAAGGA+AGG + chr2.2:2087981-2088000 None:intergenic 10.0%
!! AATATAGTATTATTATTGAA+GGG - chr2.2:2087945-2087964 MS.gene36333:intron 10.0%
!! ATATATATATATATATAGGA+GGG + chr2.2:2088473-2088492 None:intergenic 10.0%
!! TATATATATATATATATAGG+AGG + chr2.2:2088474-2088493 None:intergenic 10.0%
!!! TTTTATAAAAAACTATTAAC+AGG - chr2.2:2087997-2088016 MS.gene36333:intron 10.0%
!! AAAATAGGTCATATATATAT+AGG - chr2.2:2086788-2086807 MS.gene36333:intron 15.0%
!! AATGAATTCTAATTAATTGA+TGG + chr2.2:2088083-2088102 None:intergenic 15.0%
!! AATTCATTAGAATCAAATTA+AGG - chr2.2:2088095-2088114 MS.gene36333:CDS 15.0%
!! ATTCATTAGAATCAAATTAA+GGG - chr2.2:2088096-2088115 MS.gene36333:CDS 15.0%
!! GAATATAGTATTATTATTGA+AGG - chr2.2:2087944-2087963 MS.gene36333:intron 15.0%
!! TATATATATATATATAGGAG+GGG + chr2.2:2088472-2088491 None:intergenic 15.0%
!!! AATGTCACTTTAATAAATAA+TGG - chr2.2:2088828-2088847 MS.gene36333:intron 15.0%
!!! TTTTCTTCATTATAATTACA+TGG - chr2.2:2086384-2086403 MS.gene36333:intron 15.0%
!! AAAAGAAAAAAAAGAATACC+TGG + chr2.2:2088397-2088416 None:intergenic 20.0%
!! AAATTCTTATCCTTAAGTAT+AGG - chr2.2:2087204-2087223 MS.gene36333:CDS 20.0%
!! AACATTCTTTCTTTAGTTAT+AGG - chr2.2:2087736-2087755 MS.gene36333:CDS 20.0%
!! ACAATGGTTTATCATAATTA+TGG + chr2.2:2087354-2087373 None:intergenic 20.0%
!! CTTAAGTAGTGATAATATAT+TGG - chr2.2:2086702-2086721 MS.gene36333:intron 20.0%
!! GAAGAAAAAAAACATGAATA+AGG + chr2.2:2086372-2086391 None:intergenic 20.0%
!! GAATTAATAGAAAAGAAAAG+AGG + chr2.2:2087029-2087048 None:intergenic 20.0%
!!! AAATATCTGAGGTAAAAAAT+AGG - chr2.2:2086773-2086792 MS.gene36333:intron 20.0%
!!! CATTGAAATTTGAAATAACA+AGG - chr2.2:2088194-2088213 MS.gene36333:intron 20.0%
!!! CTTGTTATTTCAAATTTCAA+TGG + chr2.2:2088196-2088215 None:intergenic 20.0%
!!! TAATTTTGAACCTATACTTA+AGG + chr2.2:2087217-2087236 None:intergenic 20.0%
!!! TGCTTTATTATATCCATAAA+TGG + chr2.2:2086547-2086566 None:intergenic 20.0%
! AAAAAAAAGAATACCTGGTT+TGG + chr2.2:2088392-2088411 None:intergenic 25.0%
! AACCAAATTAACACAAACAA+TGG + chr2.2:2087370-2087389 None:intergenic 25.0%
! AACCATTGTTTGTGTTAATT+TGG - chr2.2:2087365-2087384 MS.gene36333:intron 25.0%
! ATACTAAAATCATGATTCCA+TGG + chr2.2:2088435-2088454 None:intergenic 25.0%
! CAAAAAGAATATTACAGTGT+TGG + chr2.2:2087133-2087152 None:intergenic 25.0%
! GAAATTGAATAGGACAAAAA+TGG - chr2.2:2087891-2087910 MS.gene36333:intron 25.0%
! TACTAAAATCATGATTCCAT+GGG + chr2.2:2088434-2088453 None:intergenic 25.0%
! TATAATAAACAGAATGCAGA+AGG - chr2.2:2088853-2088872 MS.gene36333:CDS 25.0%
! TATCATCAAGTGCATAAATT+GGG - chr2.2:2088663-2088682 MS.gene36333:intron 25.0%
! TTCAATCAATGACAAAAGAT+AGG + chr2.2:2087465-2087484 None:intergenic 25.0%
!! AGAATTTTTATGGTCAACAA+GGG - chr2.2:2086584-2086603 MS.gene36333:intron 25.0%
!!! ACTTTTCTCTGAAATTGAAT+AGG - chr2.2:2087881-2087900 MS.gene36333:intron 25.0%
AAAGATAGGTACACAGAAAT+TGG + chr2.2:2087451-2087470 None:intergenic 30.0%
AACCTAAAAACTCAGAAAAC+TGG - chr2.2:2086488-2086507 MS.gene36333:intron 30.0%
AACTACTGGAGTTAATAAGA+AGG - chr2.2:2086936-2086955 MS.gene36333:intron 30.0%
AAGAAAGAAAGATGAGTCTT+TGG - chr2.2:2087511-2087530 MS.gene36333:intron 30.0%
ACCTAAAAACTCAGAAAACT+GGG - chr2.2:2086489-2086508 MS.gene36333:intron 30.0%
ACTAAAATCATGATTCCATG+GGG + chr2.2:2088433-2088452 None:intergenic 30.0%
ATAAAGCATGAAGTTTCTCA+AGG - chr2.2:2086559-2086578 MS.gene36333:intron 30.0%
ATAAATGGAACTCTTGAAGA+TGG + chr2.2:2086532-2086551 None:intergenic 30.0%
ATCTTCAAGAGTTCCATTTA+TGG - chr2.2:2086531-2086550 MS.gene36333:intron 30.0%
ATTACATGTGAATCTGAATC+AGG - chr2.2:2087242-2087261 MS.gene36333:intron 30.0%
ATTGGATTTCACTTACAACA+AGG - chr2.2:2086720-2086739 MS.gene36333:intron 30.0%
GAAAACAACTATTTCATGTG+TGG + chr2.2:2087405-2087424 None:intergenic 30.0%
GTAGTAATAACTGTAAAGAG+AGG + chr2.2:2088129-2088148 None:intergenic 30.0%
GTATCATCAAGTGCATAAAT+TGG - chr2.2:2088662-2088681 MS.gene36333:intron 30.0%
TCTGTATTATTAGAAGCCAT+TGG - chr2.2:2087562-2087581 MS.gene36333:intron 30.0%
TGTGAGTAATTAACCTTTAG+AGG + chr2.2:2086991-2087010 None:intergenic 30.0%
TTTACCTCAGATATTTGATC+TGG + chr2.2:2086769-2086788 None:intergenic 30.0%
TTTCTGTCAAGGAATTGAAA+GGG + chr2.2:2086134-2086153 None:intergenic 30.0%
! ACATGGTGTGAAAATTTTGA+GGG - chr2.2:2086401-2086420 MS.gene36333:intron 30.0%
! ATGCACTTGATGATACTATA+TGG + chr2.2:2088658-2088677 None:intergenic 30.0%
! CTACAGTATAGAAGATTTTG+AGG + chr2.2:2086301-2086320 None:intergenic 30.0%
! GAGAATTTTTATGGTCAACA+AGG - chr2.2:2086583-2086602 MS.gene36333:intron 30.0%
! GCAACTGTGATTTAAAATTG+TGG + chr2.2:2086256-2086275 None:intergenic 30.0%
! GTGATTTAAAATTGTGGTGA+TGG + chr2.2:2086250-2086269 None:intergenic 30.0%
! TACATGGTGTGAAAATTTTG+AGG - chr2.2:2086400-2086419 MS.gene36333:intron 30.0%
! TCTCAAGGTGAGAATTTTTA+TGG - chr2.2:2086574-2086593 MS.gene36333:intron 30.0%
! TGAATTTTCTGTGAGTTGTT+AGG - chr2.2:2088754-2088773 MS.gene36333:intron 30.0%
! TTCAGCATTATTTTCTGTCA+AGG + chr2.2:2086145-2086164 None:intergenic 30.0%
! TTTTCTGTCAAGGAATTGAA+AGG + chr2.2:2086135-2086154 None:intergenic 30.0%
! TTTTGTATGTTTTGCAGTAC+TGG - chr2.2:2086424-2086443 MS.gene36333:intron 30.0%
!! TAGTTTGTTACCTTTCCAAA+TGG + chr2.2:2087321-2087340 None:intergenic 30.0%
ACAGAATGCAGAAGGTAATA+AGG - chr2.2:2088861-2088880 MS.gene36333:CDS 35.0%
ACTACCAGATCAAATATCTG+AGG - chr2.2:2086762-2086781 MS.gene36333:intron 35.0%
AGCATTTGAACACATGTAGT+AGG + chr2.2:2088802-2088821 None:intergenic 35.0%
ATCCAAGCATATATTGAGGA+GGG + chr2.2:2086101-2086120 None:intergenic 35.0%
CAACATGTTGGAAGTGAAAA+TGG - chr2.2:2088934-2088953 MS.gene36333:CDS 35.0%
CAGATATTTGATCTGGTAGT+TGG + chr2.2:2086762-2086781 None:intergenic 35.0%
CAGCATCAAAAAACATGATG+AGG - chr2.2:2087821-2087840 MS.gene36333:intron 35.0%
GAAGATCCAAGCATATATTG+AGG + chr2.2:2086105-2086124 None:intergenic 35.0%
GTTTCTACAGAGTCAAATTG+AGG - chr2.2:2087594-2087613 MS.gene36333:intron 35.0%
TAGAAACCTGATGTATCCAA+TGG + chr2.2:2087581-2087600 None:intergenic 35.0%
TAGTGTAATAGCTCAACTAC+TGG - chr2.2:2086922-2086941 MS.gene36333:intron 35.0%
TGAAAATGGAGCTGATTACT+GGG - chr2.2:2088948-2088967 MS.gene36333:CDS 35.0%
TGGAGTTAATAAGAAGGCTA+GGG - chr2.2:2086942-2086961 MS.gene36333:intron 35.0%
TTCAGAGAAAAGTGAAGCAT+TGG + chr2.2:2087874-2087893 None:intergenic 35.0%
! AATTAACCTTTAGAGGTGGT+TGG + chr2.2:2086984-2087003 None:intergenic 35.0%
! GAGTAATTAACCTTTAGAGG+TGG + chr2.2:2086988-2087007 None:intergenic 35.0%
! TCAGAGAAAAGTGAAGCATT+GGG + chr2.2:2087873-2087892 None:intergenic 35.0%
! TCCCAGTTTTCTGAGTTTTT+AGG + chr2.2:2086493-2086512 None:intergenic 35.0%
! TTATTCCTCTCTTTTGTTGC+AGG - chr2.2:2087771-2087790 MS.gene36333:intron 35.0%
! TTTTATTCCCATCTAGCTGT+TGG - chr2.2:2088331-2088350 MS.gene36333:CDS 35.0%
!! AAGAATACCTGGTTTGGAAT+TGG + chr2.2:2088386-2088405 None:intergenic 35.0%
!!! CAAGCTACGGTTTTAAATTG+CGG + chr2.2:2086331-2086350 None:intergenic 35.0%
AAGCTAGTGATATGAGAGCT+TGG + chr2.2:2086685-2086704 None:intergenic 40.0%
AGCTAGTGATATGAGAGCTT+GGG + chr2.2:2086684-2086703 None:intergenic 40.0%
ATCCCTCCTCAATATATGCT+TGG - chr2.2:2086096-2086115 MS.gene36333:CDS 40.0%
ATGTGAATCTGAATCAGGTG+AGG - chr2.2:2087247-2087266 MS.gene36333:intron 40.0%
CAAAGGACTTGAAGAGTAGA+GGG - chr2.2:2088884-2088903 MS.gene36333:CDS 40.0%
CTGGAGTTAATAAGAAGGCT+AGG - chr2.2:2086941-2086960 MS.gene36333:intron 40.0%
GATCCAAGCATATATTGAGG+AGG + chr2.2:2086102-2086121 None:intergenic 40.0%
GATGCTGTAACTTCAAGCTA+CGG + chr2.2:2086344-2086363 None:intergenic 40.0%
GGAGGGATATTCAAAGATGA+TGG + chr2.2:2086084-2086103 None:intergenic 40.0%
GTGAAAATGGAGCTGATTAC+TGG - chr2.2:2088947-2088966 MS.gene36333:CDS 40.0%
TAAAACCCACTTCCAAATGC+TGG + chr2.2:2088976-2088995 None:intergenic 40.0%
TAGAAGCCATTGGATACATC+AGG - chr2.2:2087572-2087591 MS.gene36333:intron 40.0%
TCCAATAAAGCATACCTGAC+CGG + chr2.2:2088059-2088078 None:intergenic 40.0%
TCTGTGAGTTGTTAGGTCAT+TGG - chr2.2:2088761-2088780 MS.gene36333:intron 40.0%
TGCAGAAGGTAATAAGGCAA+AGG - chr2.2:2088867-2088886 MS.gene36333:CDS 40.0%
TGGTTCATCTTGGTCACATA+TGG - chr2.2:2086645-2086664 MS.gene36333:CDS 40.0%
TTCCAAATGGAGAAACTAGC+TGG + chr2.2:2087308-2087327 None:intergenic 40.0%
! AGTAGTTGACTCCATGATTG+AGG + chr2.2:2086174-2086193 None:intergenic 40.0%
! GCTAGTTTCTCCATTTGGAA+AGG - chr2.2:2087308-2087327 MS.gene36333:intron 40.0%
!! TTGGAGCGCTCTTTCTTTTT+AGG + chr2.2:2088367-2088386 None:intergenic 40.0%
ACATGTTGTGTGCATGACAC+TGG + chr2.2:2088922-2088941 None:intergenic 45.0%
AGAGAGAGAGAGAGAGAGTA+TGG + chr2.2:2086210-2086229 None:intergenic 45.0%
AGTGTCATCCAACAGCTAGA+TGG + chr2.2:2088342-2088361 None:intergenic 45.0%
ATCTAGCTGTTGGATGACAC+TGG - chr2.2:2088341-2088360 MS.gene36333:CDS 45.0%
ATGCTGCAGTGTCCAAGTAA+GGG + chr2.2:2087807-2087826 None:intergenic 45.0%
CAATAAAGCATACCTGACCG+GGG + chr2.2:2088057-2088076 None:intergenic 45.0%
CATGCTAACTCTGGATCACT+TGG - chr2.2:2086625-2086644 MS.gene36333:intron 45.0%
CCAATAAAGCATACCTGACC+GGG + chr2.2:2088058-2088077 None:intergenic 45.0%
CTGGATCACTTGGTTCATCT+TGG - chr2.2:2086635-2086654 MS.gene36333:intron 45.0%
GAATCTGAATCAGGTGAGGA+AGG - chr2.2:2087251-2087270 MS.gene36333:intron 45.0%
GAGGAGTTTCATGCTAACTC+TGG - chr2.2:2086616-2086635 MS.gene36333:intron 45.0%
GCAAAGGACTTGAAGAGTAG+AGG - chr2.2:2088883-2088902 MS.gene36333:CDS 45.0%
GCTAGTGATATGAGAGCTTG+GGG + chr2.2:2086683-2086702 None:intergenic 45.0%
GTGTCATCCAACAGCTAGAT+GGG + chr2.2:2088341-2088360 None:intergenic 45.0%
GTGTCATGCACACAACATGT+TGG - chr2.2:2088922-2088941 MS.gene36333:CDS 45.0%
TCTGTATTTCCTGAAGACCC+CGG - chr2.2:2088037-2088056 MS.gene36333:intron 45.0%
TTTCTACAAAAGCTGCCCCA+TGG - chr2.2:2088415-2088434 MS.gene36333:CDS 45.0%
! AGTTGACTCCATGATTGAGG+AGG + chr2.2:2086171-2086190 None:intergenic 45.0%
! CACCAGCTAGTTTCTCCATT+TGG - chr2.2:2087303-2087322 MS.gene36333:intron 45.0%
! CTGAAGTACCTCCTCAATCA+TGG - chr2.2:2086160-2086179 MS.gene36333:intron 45.0%
!! TCAACAAGGGCATCATCATG+AGG - chr2.2:2086597-2086616 MS.gene36333:intron 45.0%
!! TTTTGCAGTACTGGACTACC+TGG - chr2.2:2086433-2086452 MS.gene36333:intron 45.0%
!! ATAAATAAATAAGAATAATA+TGG + chr2.2:2087628-2087647 None:intergenic 5.0%
CCAAGTCTCTCTTCTTCACC+AGG + chr2.2:2086454-2086473 None:intergenic 50.0%
CCCGGTCAGGTATGCTTTAT+TGG - chr2.2:2088055-2088074 MS.gene36333:CDS 50.0%
CCTGGTGAAGAAGAGAGACT+TGG - chr2.2:2086451-2086470 MS.gene36333:intron 50.0%
CTCAAGCCAACCACCTCTAA+AGG - chr2.2:2086975-2086994 MS.gene36333:intron 50.0%
GAAGAGTAGAGGGTTGTGCT+TGG - chr2.2:2088894-2088913 MS.gene36333:CDS 50.0%
GAGTGCCTGCAACAAAAGAG+AGG + chr2.2:2087779-2087798 None:intergenic 50.0%
GATGCATGCTCCACCAGTTA+GGG + chr2.2:2086062-2086081 None:intergenic 50.0%
GATGCTGCAGTGTCCAAGTA+AGG + chr2.2:2087808-2087827 None:intergenic 50.0%
GATTACTGGGCACCAGCATT+TGG - chr2.2:2088961-2088980 MS.gene36333:CDS 50.0%
TGAGAGCTTGGGGAAGAAAC+TGG + chr2.2:2086673-2086692 None:intergenic 50.0%
TGCTGCAGTGTCCAAGTAAG+GGG + chr2.2:2087806-2087825 None:intergenic 50.0%
!! CTTTAGAGGTGGTTGGCTTG+AGG + chr2.2:2086977-2086996 None:intergenic 50.0%
ATTTCCTGAAGACCCCGGTC+AGG - chr2.2:2088042-2088061 MS.gene36333:intron 55.0%
CAGGTGAGGAAGGAGAAGCT+AGG - chr2.2:2087261-2087280 MS.gene36333:intron 55.0%
GAGCGCTCCAATTCCAAACC+AGG - chr2.2:2088376-2088395 MS.gene36333:CDS 55.0%
GGATGCATGCTCCACCAGTT+AGG + chr2.2:2086063-2086082 None:intergenic 55.0%
GGGCACCAGCATTTGGAAGT+GGG - chr2.2:2088968-2088987 MS.gene36333:CDS 55.0%
TGATGGCTGGGAACCCTAAC+TGG - chr2.2:2086046-2086065 MS.gene36333:CDS 55.0%
TGGGCACCAGCATTTGGAAG+TGG - chr2.2:2088967-2088986 MS.gene36333:CDS 55.0%
!! AGAGGTGGTTGGCTTGAGGA+TGG + chr2.2:2086973-2086992 None:intergenic 55.0%
CATACCTGACCGGGGTCTTC+AGG + chr2.2:2088049-2088068 None:intergenic 60.0%
GGCACTCAGCACCCCTTACT+TGG - chr2.2:2087792-2087811 MS.gene36333:intron 60.0%
TGGCTGGGAACCCTAACTGG+TGG - chr2.2:2086049-2086068 MS.gene36333:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 2086045 2088996 2086045 ID=MS.gene36333
chr2.2 mRNA 2086045 2088996 2086045 ID=MS.gene36333.t1;Parent=MS.gene36333
chr2.2 exon 2088845 2088996 2088845 ID=MS.gene36333.t1.exon1;Parent=MS.gene36333.t1
chr2.2 CDS 2088845 2088996 2088845 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2088258 2088600 2088258 ID=MS.gene36333.t1.exon2;Parent=MS.gene36333.t1
chr2.2 CDS 2088258 2088600 2088258 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2088045 2088116 2088045 ID=MS.gene36333.t1.exon3;Parent=MS.gene36333.t1
chr2.2 CDS 2088045 2088116 2088045 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2087712 2087777 2087712 ID=MS.gene36333.t1.exon4;Parent=MS.gene36333.t1
chr2.2 CDS 2087712 2087777 2087712 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2087426 2087491 2087426 ID=MS.gene36333.t1.exon5;Parent=MS.gene36333.t1
chr2.2 CDS 2087426 2087491 2087426 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2087186 2087248 2087186 ID=MS.gene36333.t1.exon6;Parent=MS.gene36333.t1
chr2.2 CDS 2087186 2087248 2087186 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2086978 2087022 2086978 ID=MS.gene36333.t1.exon7;Parent=MS.gene36333.t1
chr2.2 CDS 2086978 2087022 2086978 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2086644 2086694 2086644 ID=MS.gene36333.t1.exon8;Parent=MS.gene36333.t1
chr2.2 CDS 2086644 2086694 2086644 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
chr2.2 exon 2086045 2086176 2086045 ID=MS.gene36333.t1.exon9;Parent=MS.gene36333.t1
chr2.2 CDS 2086045 2086176 2086045 ID=cds.MS.gene36333.t1;Parent=MS.gene36333.t1
Gene Sequence

>MS.gene36333

ATGATGGCTGGGAACCCTAACTGGTGGAGCATGCATCCATCATCTTTGAATATCCCTCCTCAATATATGCTTGGATCTTCTTCAATCCCTTTCAATTCCTTGACAGAAAATAATGCTGAAGTACCTCCTCAATCATGGAGTCAACTACTTTTGTTAGTAAAACCATACTCTCTCTCTCTCTCTCTAGCTTCTTAATTTGTGTCCATCACCACAATTTTAAATCACAGTTGCAGTTAAGCTACAGTTACAGTGACCTCAAAATCTTCTATACTGTAGTTGCAGACCGCAATTTAAAACCGTAGCTTGAAGTTACAGCATCTTTGACCTTATTCATGTTTTTTTTCTTCATTATAATTACATGGTGTGAAAATTTTGAGGGTTTTGTATGTTTTGCAGTACTGGACTACCTGGTGAAGAAGAGAGACTTGGTTTTAATCATTTTCAACCTAAAAACTCAGAAAACTGGGATGTGCAAATTATGAATCCATCTTCAAGAGTTCCATTTATGGATATAATAAAGCATGAAGTTTCTCAAGGTGAGAATTTTTATGGTCAACAAGGGCATCATCATGAGGAGTTTCATGCTAACTCTGGATCACTTGGTTCATCTTGGTCACATATGGTGCCAGTTTCTTCCCCAAGCTCTCATATCACTAGCTTAAGTAGTGATAATATATTGGATTTCACTTACAACAAGGTAGATCATAGCAAGAACCAACTACCAGATCAAATATCTGAGGTAAAAAATAGGTCATATATATATAGGTTCTTGTTAATTATTATTTAATTTTTCTCAACTTCTAATATTGCTATTTATTAATATAAGTCATGTATATATGATATTCAAATTAATTTCATCTATCTCTTTCATATGCTCTAGTGTAATAGCTCAACTACTGGAGTTAATAAGAAGGCTAGGGTTCAGCCATCCTCAAGCCAACCACCTCTAAAGGTTAATTACTCACAAATTAACGCTTTTAACCTCTTTTCTTTTCTATTAATTCTATCTTAACTACTTAGTAATTAGTGAGCTTGTATATATGTGTATGTTTTTTTGTTACTGTTTTTCTTGTTTGTCTTACTCTCCAACACTGTAATATTCTTTTTGCTGATATTCATATATATACTTGTTGAATGAGAGAAATTATTTAAGCATATGAAATTCTTATCCTTAAGTATAGGTTCAAAATTATACTAATTACATGTGAATCTGAATCAGGTGAGGAAGGAGAAGCTAGGTGATAGAATAACAGCACTTCACCAGCTAGTTTCTCCATTTGGAAAGGTAACAAACTAATTAATCACTCCATAATTATGATAAACCATTGTTTGTGTTAATTTGGTTTTATAAAAAAGTCCACACATGAAATAGTTGTTTTCAATTCAAAGTAGACTTTGATGATCCAATTTCTGTGTACCTATCTTTTGTCATTGATTGAAATTTTACTTTTAACATTAAAAGAAGAAAGAAAGAAAGATGAGTCTTTGGTGCTTCACTTTTGCAGACTGACACAGCTTCTGTATTATTAGAAGCCATTGGATACATCAGGTTTCTACAGAGTCAAATTGAGGTGATGTTTCCATATTATTCTTATTTATTTATTAATTATACTATGCAGCAAGTTAGTACTGTATTTCAGAACATGCAGTTAAAGTTTGTTCAATTTTGATGTGATTTTTTTCACTCTAAAAACATTCTTTCTTTAGTTATAGGAGTTATAAAATTTTATTCCTCTCTTTTGTTGCAGGCACTCAGCACCCCTTACTTGGACACTGCAGCATCAAAAAACATGATGAGGAATCAACATTCTGTAAGTTCACATTTCCCAATGCTTCACTTTTCTCTGAAATTGAATAGGACAAAAATGGAGTATACTTAATGTCTTTGTTAGAATTATAGAATATAGTATTATTATTGAAGGGAATGTTTGTTCCTTCCTTTTTTAATTTTATTTTATAAAAAACTATTAACAGGTGCATGTTGAAAGAAATTCTGTATTTCCTGAAGACCCCGGTCAGGTATGCTTTATTGGATCCATCAATTAATTAGAATTCATTAGAATCAAATTAAGGGAAATTAACCTCTCTTTACAGTTATTACTACACTATTTAATTAATTAATTAATATTAATATGCTCAAGAAAGAAGCCATTGAAATTTGAAATAACAAGGTTTCTCAGCTTTTTATATAACATCTTTTAGAAAAAGCATGATGAAGCTGAATTGTTCACTTGAAGTCAAATTTCATAGAGAAATGAAATTAGTAAATAATATATACTTTCTTCTTTTTATTCCCATCTAGCTGTTGGATGACACTGGCCTAAAAAGAAAGAGCGCTCCAATTCCAAACCAGGTATTCTTTTTTTTCTTTTTCTACAAAAGCTGCCCCATGGAATCATGATTTTAGTATTCTTCTTCATTTGACCCCTCCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTGAAGAAATATATGTGTGTATCTTATATTTATATATAAGATTCTAATCACTCTAATAAATATTATTAAAAATAAATAATACTGTACTATATTATCATTATAACCATATAGTATCATCAAGTGCATAAATTGGGCAATGAAACTGTCAGACTTTGCATATGATTCTTATTGTTTAATTAATTAATAAAAAATAATAATGATGTGAATTTTCTGTGAGTTGTTAGGTCATTGGCAAGAGTTTCATGAACCTACTACATGTGTTCAAATGCTTAATGTAATGTCACTTTAATAAATAATGGTTTATAATAAACAGAATGCAGAAGGTAATAAGGCAAAGGACTTGAAGAGTAGAGGGTTGTGCTTGGTTCCAGTGTCATGCACACAACATGTTGGAAGTGAAAATGGAGCTGATTACTGGGCACCAGCATTTGGAAGTGGGTTTTAA
Protein sequence

>MS.gene36333.t1

MMAGNPNWWSMHPSSLNIPPQYMLGSSSIPFNSLTENNAEVPPQSWSQLLFTGLPGEEERLGFNHFQPKNSENWDVQIMNPSSRVPFMDIIKHEVSQGENFYGQQGHHHEEFHANSGSLGSSWSHMVPVSSPSSHITSLSSDNILDFTYNKVDHSKNQLPDQISECNSSTTGVNKKARVQPSSSQPPLKVRKEKLGDRITALHQLVSPFGKTDTASVLLEAIGYIRFLQSQIEALSTPYLDTAASKNMMRNQHSVHVERNSVFPEDPGQLLDDTGLKRKSAPIPNQNAEGNKAKDLKSRGLCLVPVSCTQHVGSENGADYWAPAFGSGF