Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36894.t1 | RHN40044.1 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 3.70E-100 | 374.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36894.t1 | Q7M443 | 35.0 | 274 | 151 | 9 | 14 | 280 | 2 | 255 | 6.3e-32 | 139.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36894.t1 | A0A396GKD7 | 67.9 | 296 | 69 | 10 | 1 | 290 | 1 | 276 | 2.7e-100 | 374.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051860 | MS.gene36894 | 0.826454 | 2.69E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene36894 | 0.809295 | 2.03E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene36894 | 0.812601 | 3.91E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36894.t1 | MTR_0002s0040 | 53.698 | 311 | 103 | 10 | 1 | 290 | 1 | 291 | 2.50e-95 | 283 |
| MS.gene36894.t1 | MTR_0263s0020 | 56.164 | 292 | 96 | 9 | 1 | 280 | 1 | 272 | 4.21e-94 | 280 |
| MS.gene36894.t1 | MTR_1457s0010 | 66.234 | 231 | 54 | 8 | 65 | 291 | 1 | 211 | 2.88e-93 | 275 |
| MS.gene36894.t1 | MTR_0002s1060 | 51.546 | 291 | 108 | 11 | 4 | 280 | 6 | 277 | 3.70e-75 | 232 |
| MS.gene36894.t1 | MTR_0062s0020 | 38.542 | 288 | 140 | 8 | 14 | 280 | 4 | 275 | 1.39e-48 | 164 |
| MS.gene36894.t1 | MTR_0062s0090 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene36894.t1 | MTR_0062s0040 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene36894.t1 | MTR_0062s0060 | 39.510 | 286 | 138 | 10 | 14 | 280 | 4 | 273 | 8.98e-48 | 161 |
| MS.gene36894.t1 | MTR_0443s0040 | 39.175 | 291 | 132 | 12 | 14 | 280 | 16 | 285 | 1.26e-47 | 161 |
| MS.gene36894.t1 | MTR_0062s0170 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_0062s0110 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_0062s0130 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_2g036240 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_2g036260 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_2g036290 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_8g046730 | 36.304 | 303 | 151 | 10 | 1 | 280 | 1 | 284 | 8.30e-39 | 139 |
| MS.gene36894.t1 | MTR_6g079630 | 34.182 | 275 | 156 | 8 | 12 | 280 | 32 | 287 | 3.22e-37 | 134 |
| MS.gene36894.t1 | MTR_2g096030 | 32.990 | 291 | 163 | 10 | 14 | 291 | 31 | 302 | 1.39e-33 | 124 |
| MS.gene36894.t1 | MTR_2g096040 | 33.202 | 253 | 145 | 6 | 39 | 283 | 26 | 262 | 2.08e-28 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | - | 57511:16020-16039 | MS.gene36894:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | - | 57511:16025-16044 | MS.gene36894:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | - | 57511:16026-16045 | MS.gene36894:intergenic | 25.0% |
| !! | TTCATTTTACGAGAATACAT+TGG | + | 57511:15987-16006 | MS.gene36894:CDS | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | - | 57511:16279-16298 | MS.gene36894:intergenic | 30.0% | |
| ATTTCGTTGTAGTCAATATC+AGG | - | 57511:16044-16063 | MS.gene36894:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | - | 57511:16280-16299 | MS.gene36894:intergenic | 30.0% | |
| TATAAAAAGACTCAAGGAAG+AGG | + | 57511:16442-16461 | MS.gene36894:CDS | 30.0% | |
| TTTCGTTGTAGTCAATATCA+GGG | - | 57511:16043-16062 | MS.gene36894:intergenic | 30.0% | |
| ! | GTAGTGTTCATTAGTTTCAT+GGG | - | 57511:16498-16517 | MS.gene36894:intergenic | 30.0% |
| ! | TGTAGTGTTCATTAGTTTCA+TGG | - | 57511:16499-16518 | MS.gene36894:intergenic | 30.0% |
| !! | AATCGATTTCCATGTCATTT+TGG | + | 57511:16061-16080 | MS.gene36894:CDS | 30.0% |
| !! | ACTTTTGAAAAACTCCTAGT+AGG | + | 57511:16647-16666 | MS.gene36894:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | - | 57511:16293-16312 | MS.gene36894:intergenic | 30.0% |
| AAGATTTCTCCAACTACGTT+GGG | + | 57511:16414-16433 | MS.gene36894:CDS | 35.0% | |
| ACCACAGTGACGATATTAAT+TGG | + | 57511:16534-16553 | MS.gene36894:CDS | 35.0% | |
| ACTTACAACGAAGATGGAAA+AGG | + | 57511:16098-16117 | MS.gene36894:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | - | 57511:16093-16112 | MS.gene36894:intergenic | 35.0% | |
| CCACAGTGACGATATTAATT+GGG | + | 57511:16535-16554 | MS.gene36894:CDS | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | + | 57511:16297-16316 | MS.gene36894:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | - | 57511:16278-16297 | MS.gene36894:intergenic | 35.0% | |
| GGACGTTATAAAAAGACTCA+AGG | + | 57511:16436-16455 | MS.gene36894:CDS | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | - | 57511:16281-16300 | MS.gene36894:intergenic | 35.0% | |
| GTTGGAGAAATCTTCTTCAT+TGG | - | 57511:16408-16427 | MS.gene36894:intergenic | 35.0% | |
| ! | TGTTCATTAGTTTCATGGGA+TGG | - | 57511:16494-16513 | MS.gene36894:intergenic | 35.0% |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | - | 57511:16302-16321 | MS.gene36894:intergenic | 35.0% |
| AAAAGACTCAAGGAAGAGGT+AGG | + | 57511:16446-16465 | MS.gene36894:CDS | 40.0% | |
| AACACTACAAGCTACTCTAC+AGG | + | 57511:16510-16529 | MS.gene36894:CDS | 40.0% | |
| ACACTACAAGCTACTCTACA+GGG | + | 57511:16511-16530 | MS.gene36894:CDS | 40.0% | |
| AGATTTCTCCAACTACGTTG+GGG | + | 57511:16415-16434 | MS.gene36894:CDS | 40.0% | |
| ATCCAAGACTACACGTTTGA+TGG | + | 57511:16353-16372 | MS.gene36894:CDS | 40.0% | |
| CAGATATCAAGATCCCAATC+GGG | - | 57511:16140-16159 | MS.gene36894:intergenic | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | - | 57511:16094-16113 | MS.gene36894:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | + | 57511:16092-16111 | MS.gene36894:CDS | 40.0% | |
| CCCAATTAATATCGTCACTG+TGG | - | 57511:16538-16557 | MS.gene36894:intergenic | 40.0% | |
| CGGAAAATTCTATCCCGATT+GGG | + | 57511:16124-16143 | MS.gene36894:CDS | 40.0% | |
| GAAGATTTCTCCAACTACGT+TGG | + | 57511:16413-16432 | MS.gene36894:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | - | 57511:15970-15989 | MS.gene36894:intergenic | 40.0% | |
| GATCTATGTTAGACTCGCAA+GGG | + | 57511:16616-16635 | MS.gene36894:CDS | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | + | 57511:16140-16159 | MS.gene36894:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | - | 57511:16141-16160 | MS.gene36894:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | + | 57511:16181-16200 | MS.gene36894:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | + | 57511:16229-16248 | MS.gene36894:CDS | 40.0% | |
| TGATCTATGTTAGACTCGCA+AGG | + | 57511:16615-16634 | MS.gene36894:CDS | 40.0% | |
| TTGCGAGTCTAACATAGATC+AGG | - | 57511:16616-16635 | MS.gene36894:intergenic | 40.0% | |
| ! | AACGCAGTGGCATTTTCATT+TGG | + | 57511:16756-16775 | MS.gene36894:CDS | 40.0% |
| ! | CCAATCGGGATAGAATTTTC+CGG | - | 57511:16126-16145 | MS.gene36894:intergenic | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | - | 57511:16333-16352 | MS.gene36894:intergenic | 40.0% |
| ACAAAGTCAGTCTCGTCAGT+GGG | - | 57511:16590-16609 | MS.gene36894:intergenic | 45.0% | |
| ACGAAGACATCTAGTGGAAG+TGG | - | 57511:16707-16726 | MS.gene36894:intergenic | 45.0% | |
| AGTTTCATGGGATGGAGCAA+TGG | - | 57511:16486-16505 | MS.gene36894:intergenic | 45.0% | |
| CCGGAAAATTCTATCCCGAT+TGG | + | 57511:16123-16142 | MS.gene36894:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | - | 57511:16200-16219 | MS.gene36894:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | + | 57511:16247-16266 | MS.gene36894:CDS | 45.0% | |
| GCGTTGAACCTTCATGAGAT+CGG | - | 57511:16741-16760 | MS.gene36894:intergenic | 45.0% | |
| GGAAGAGGTAGGTATCAATG+TGG | + | 57511:16457-16476 | MS.gene36894:CDS | 45.0% | |
| GGTTAGGCTGCATGTAGAAT+TGG | - | 57511:16568-16587 | MS.gene36894:intergenic | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | + | 57511:16203-16222 | MS.gene36894:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | - | 57511:16087-16106 | MS.gene36894:intergenic | 45.0% | |
| TACAAAGTCAGTCTCGTCAG+TGG | - | 57511:16591-16610 | MS.gene36894:intergenic | 45.0% | |
| TATAACGTCCCCAACGTAGT+TGG | - | 57511:16426-16445 | MS.gene36894:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | - | 57511:16358-16377 | MS.gene36894:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | + | 57511:16104-16123 | MS.gene36894:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | + | 57511:16206-16225 | MS.gene36894:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | - | 57511:16170-16189 | MS.gene36894:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | - | 57511:16318-16337 | MS.gene36894:intergenic | 50.0% | |
| ATTACGTCCGTCACGTGGAT+CGG | - | 57511:16681-16700 | MS.gene36894:intergenic | 50.0% | |
| CAAAGTCAGTCTCGTCAGTG+GGG | - | 57511:16589-16608 | MS.gene36894:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | + | 57511:16190-16209 | MS.gene36894:CDS | 50.0% | |
| CTCATGAAGGTTCAACGCAG+TGG | + | 57511:16743-16762 | MS.gene36894:CDS | 50.0% | |
| CTCGCAACGAAGACATCTAG+TGG | - | 57511:16713-16732 | MS.gene36894:intergenic | 50.0% | |
| GAGTTGCTCCGATCTCATGA+AGG | + | 57511:16730-16749 | MS.gene36894:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | - | 57511:16201-16220 | MS.gene36894:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | + | 57511:16187-16206 | MS.gene36894:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | - | 57511:16073-16092 | MS.gene36894:intergenic | 50.0% | |
| GGAACATTACGTCCGTCACG+TGG | - | 57511:16686-16705 | MS.gene36894:intergenic | 55.0% | |
| TCAGTCTCGTCAGTGGGGTT+AGG | - | 57511:16584-16603 | MS.gene36894:intergenic | 55.0% | |
| ! | GATCGGTGCTTGCTCCTACT+AGG | - | 57511:16664-16683 | MS.gene36894:intergenic | 55.0% |
| CTGCGGTATACCACGTGTGC+AGG | + | 57511:16157-16176 | MS.gene36894:CDS | 60.0% | |
| !! | GCAAGCACCGATCCACGTGA+CGG | + | 57511:16671-16690 | MS.gene36894:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57511 | gene | 15951 | 16830 | 15951 | ID=MS.gene36894 |
| 57511 | mRNA | 15951 | 16830 | 15951 | ID=MS.gene36894.t1;Parent=MS.gene36894 |
| 57511 | exon | 15951 | 16830 | 15951 | ID=MS.gene36894.t1.exon1;Parent=MS.gene36894.t1 |
| 57511 | CDS | 15951 | 16830 | 15951 | ID=cds.MS.gene36894.t1;Parent=MS.gene36894.t1 |
| Gene Sequence |
| Protein sequence |
