Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37220.t1 | XP_013457650.1 | 95.4 | 151 | 7 | 0 | 1 | 151 | 119 | 269 | 2.40E-66 | 261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37220.t1 | Q50EX6 | 42.1 | 171 | 72 | 5 | 1 | 147 | 119 | 286 | 3.4e-13 | 76.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37220.t1 | A0A072UQP4 | 95.4 | 151 | 7 | 0 | 1 | 151 | 119 | 269 | 1.7e-66 | 261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene37220 | 0.805293 | 1.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37220.t1 | MTR_4g102380 | 97.351 | 151 | 4 | 0 | 1 | 151 | 119 | 269 | 4.73e-104 | 298 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37220.t1 | AT4G22680 | 37.838 | 148 | 77 | 5 | 1 | 133 | 119 | 266 | 2.12e-13 | 65.9 |
| MS.gene37220.t1 | AT4G12350 | 33.333 | 168 | 86 | 8 | 1 | 146 | 119 | 282 | 4.12e-13 | 65.5 |
Find 30 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAGTTTCCTTCACTTCA+TGG | 0.233477 | 8.3:-19292689 | None:intergenic |
| TTCACTTCATGGTTGTTAAC+AGG | 0.301922 | 8.3:-19292678 | None:intergenic |
| TCCCCAGCTGAAAATTCTTC+TGG | 0.318088 | 8.3:+19292744 | MS.gene37220:CDS |
| ACCATTGGTGGAAGCCCTTT+GGG | 0.330830 | 8.3:+19292839 | MS.gene37220:CDS |
| TTCAAAGGTTCATGAGTAAT+GGG | 0.340405 | 8.3:-19292597 | None:intergenic |
| CACCATTGGTGGAAGCCCTT+TGG | 0.357777 | 8.3:+19292838 | MS.gene37220:CDS |
| TCAAGTACACTGCAAACTAT+AGG | 0.364172 | 8.3:+19293005 | MS.gene37220:CDS |
| ATTCTCTAATGAATAGTATA+TGG | 0.364682 | 8.3:+19292805 | MS.gene37220:CDS |
| TGCTTGGCTATTTGATTGTC+AGG | 0.369569 | 8.3:+19292935 | MS.gene37220:CDS |
| GGTGGAAGCCCTTTGGGAAA+TGG | 0.381456 | 8.3:+19292845 | MS.gene37220:CDS |
| GACTTTGGTATTCATGACTT+TGG | 0.387701 | 8.3:+19292957 | MS.gene37220:CDS |
| CTATTTGATTGTCAGGACTT+TGG | 0.417724 | 8.3:+19292942 | MS.gene37220:CDS |
| ATTCAAAGGTTCATGAGTAA+TGG | 0.432527 | 8.3:-19292598 | None:intergenic |
| CAAGTACACTGCAAACTATA+GGG | 0.456076 | 8.3:+19293006 | MS.gene37220:CDS |
| GATGATGCTTGTTTATTCAA+AGG | 0.463222 | 8.3:-19292612 | None:intergenic |
| GGATGAAGTACTACTATCAT+TGG | 0.464276 | 8.3:-19292634 | None:intergenic |
| CTTGGGAGGATAATTGTGCT+TGG | 0.466595 | 8.3:+19292919 | MS.gene37220:CDS |
| TCCCAAAGGGCTTCCACCAA+TGG | 0.485135 | 8.3:-19292840 | None:intergenic |
| TGTAGTGTCCATTTCCCAAA+GGG | 0.498177 | 8.3:-19292853 | None:intergenic |
| TCTAATGAATAGTATATGGT+TGG | 0.521314 | 8.3:+19292809 | MS.gene37220:CDS |
| ACTGCAAACTATAGGGATGA+AGG | 0.532537 | 8.3:+19293013 | MS.gene37220:CDS |
| ATGAAGTGAAGGAAACTGAT+GGG | 0.557455 | 8.3:+19292691 | MS.gene37220:CDS |
| CATGAAGTGAAGGAAACTGA+TGG | 0.577027 | 8.3:+19292690 | MS.gene37220:CDS |
| GCACAATTATCCTCCCAAGA+AGG | 0.588656 | 8.3:-19292915 | None:intergenic |
| TGTTAACAACCATGAAGTGA+AGG | 0.612004 | 8.3:+19292680 | MS.gene37220:CDS |
| GTTGGATGAAACACCATTGG+TGG | 0.620513 | 8.3:+19292827 | MS.gene37220:CDS |
| GTGTAGTGTCCATTTCCCAA+AGG | 0.630743 | 8.3:-19292854 | None:intergenic |
| ATGGTTGGATGAAACACCAT+TGG | 0.670766 | 8.3:+19292824 | MS.gene37220:CDS |
| TGATGGGGTTGTCAACTCAG+AGG | 0.704259 | 8.3:+19292707 | MS.gene37220:CDS |
| TGAAGTGAAGGAAACTGATG+GGG | 0.739601 | 8.3:+19292692 | MS.gene37220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTCTAATGAATAGTATA+TGG | + | chr8.3:19292805-19292824 | MS.gene37220:CDS | 20.0% |
| ! | TCTAATGAATAGTATATGGT+TGG | + | chr8.3:19292809-19292828 | MS.gene37220:CDS | 25.0% |
| !!! | TTTTTTGTGTTTTCTGCTAT+AGG | - | chr8.3:19292879-19292898 | None:intergenic | 25.0% |
| ATTCAAAGGTTCATGAGTAA+TGG | - | chr8.3:19292601-19292620 | None:intergenic | 30.0% | |
| GATGATGCTTGTTTATTCAA+AGG | - | chr8.3:19292615-19292634 | None:intergenic | 30.0% | |
| TTCAAAGGTTCATGAGTAAT+GGG | - | chr8.3:19292600-19292619 | None:intergenic | 30.0% | |
| ATGAAGTGAAGGAAACTGAT+GGG | + | chr8.3:19292691-19292710 | MS.gene37220:CDS | 35.0% | |
| CAAGTACACTGCAAACTATA+GGG | + | chr8.3:19293006-19293025 | MS.gene37220:CDS | 35.0% | |
| CTATTTGATTGTCAGGACTT+TGG | + | chr8.3:19292942-19292961 | MS.gene37220:CDS | 35.0% | |
| GACTTTGGTATTCATGACTT+TGG | + | chr8.3:19292957-19292976 | MS.gene37220:CDS | 35.0% | |
| TCAAGTACACTGCAAACTAT+AGG | + | chr8.3:19293005-19293024 | MS.gene37220:CDS | 35.0% | |
| TGTTAACAACCATGAAGTGA+AGG | + | chr8.3:19292680-19292699 | MS.gene37220:CDS | 35.0% | |
| TTCACTTCATGGTTGTTAAC+AGG | - | chr8.3:19292681-19292700 | None:intergenic | 35.0% | |
| ! | GGATGAAGTACTACTATCAT+TGG | - | chr8.3:19292637-19292656 | None:intergenic | 35.0% |
| !! | ACATGAGTTTTATGCCTTCT+TGG | + | chr8.3:19292901-19292920 | MS.gene37220:CDS | 35.0% |
| !! | CATGAGTTTTATGCCTTCTT+GGG | + | chr8.3:19292902-19292921 | MS.gene37220:CDS | 35.0% |
| ACTGCAAACTATAGGGATGA+AGG | + | chr8.3:19293013-19293032 | MS.gene37220:CDS | 40.0% | |
| ATGGTTGGATGAAACACCAT+TGG | + | chr8.3:19292824-19292843 | MS.gene37220:CDS | 40.0% | |
| CATCAGTTTCCTTCACTTCA+TGG | - | chr8.3:19292692-19292711 | None:intergenic | 40.0% | |
| CATGAAGTGAAGGAAACTGA+TGG | + | chr8.3:19292690-19292709 | MS.gene37220:CDS | 40.0% | |
| TGAAGTGAAGGAAACTGATG+GGG | + | chr8.3:19292692-19292711 | MS.gene37220:CDS | 40.0% | |
| TGCTTGGCTATTTGATTGTC+AGG | + | chr8.3:19292935-19292954 | MS.gene37220:CDS | 40.0% | |
| TGTAGTGTCCATTTCCCAAA+GGG | - | chr8.3:19292856-19292875 | None:intergenic | 40.0% | |
| ! | CTCCAGAAGAATTTTCAGCT+GGG | - | chr8.3:19292749-19292768 | None:intergenic | 40.0% |
| ! | TCCAGAAGAATTTTCAGCTG+GGG | - | chr8.3:19292748-19292767 | None:intergenic | 40.0% |
| ! | TCTCCAGAAGAATTTTCAGC+TGG | - | chr8.3:19292750-19292769 | None:intergenic | 40.0% |
| CTTGGGAGGATAATTGTGCT+TGG | + | chr8.3:19292919-19292938 | MS.gene37220:CDS | 45.0% | |
| GCACAATTATCCTCCCAAGA+AGG | - | chr8.3:19292918-19292937 | None:intergenic | 45.0% | |
| GTGTAGTGTCCATTTCCCAA+AGG | - | chr8.3:19292857-19292876 | None:intergenic | 45.0% | |
| GTTGGATGAAACACCATTGG+TGG | + | chr8.3:19292827-19292846 | MS.gene37220:CDS | 45.0% | |
| TCCCCAGCTGAAAATTCTTC+TGG | + | chr8.3:19292744-19292763 | MS.gene37220:CDS | 45.0% | |
| ! | CTGTGACGAATTTTCTGCAG+GGG | - | chr8.3:19292658-19292677 | None:intergenic | 45.0% |
| ! | GCTGTGACGAATTTTCTGCA+GGG | - | chr8.3:19292659-19292678 | None:intergenic | 45.0% |
| !! | GAGTTTTATGCCTTCTTGGG+AGG | + | chr8.3:19292905-19292924 | MS.gene37220:CDS | 45.0% |
| ACCATTGGTGGAAGCCCTTT+GGG | + | chr8.3:19292839-19292858 | MS.gene37220:CDS | 50.0% | |
| TGATGGGGTTGTCAACTCAG+AGG | + | chr8.3:19292707-19292726 | MS.gene37220:CDS | 50.0% | |
| ! | GGCTGTGACGAATTTTCTGC+AGG | - | chr8.3:19292660-19292679 | None:intergenic | 50.0% |
| CACCATTGGTGGAAGCCCTT+TGG | + | chr8.3:19292838-19292857 | MS.gene37220:CDS | 55.0% | |
| TCCCAAAGGGCTTCCACCAA+TGG | - | chr8.3:19292843-19292862 | None:intergenic | 55.0% | |
| ! | GGTGGAAGCCCTTTGGGAAA+TGG | + | chr8.3:19292845-19292864 | MS.gene37220:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19292585 | 19293043 | 19292585 | ID=MS.gene37220 |
| chr8.3 | mRNA | 19292585 | 19293043 | 19292585 | ID=MS.gene37220.t1;Parent=MS.gene37220 |
| chr8.3 | exon | 19292585 | 19293043 | 19292585 | ID=MS.gene37220.t1.exon1;Parent=MS.gene37220.t1 |
| chr8.3 | CDS | 19292585 | 19293043 | 19292585 | ID=cds.MS.gene37220.t1;Parent=MS.gene37220.t1 |
| Gene Sequence |
| Protein sequence |
