Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37499.t1 | RHN40117.1 | 94.3 | 1193 | 52 | 4 | 1 | 1184 | 1 | 1186 | 0.00E+00 | 2142.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37499.t1 | F4K1G2 | 50.9 | 1056 | 474 | 10 | 143 | 1173 | 2 | 1037 | 5.1e-266 | 919.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37499.t1 | A0A396GN35 | 94.3 | 1193 | 52 | 4 | 1 | 1184 | 1 | 1186 | 0.0e+00 | 2142.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene37499 | MS.gene065345 | PPI |
| MS.gene37499 | MS.gene057442 | PPI |
| MS.gene37499 | MS.gene025293 | PPI |
| MS.gene37499 | MS.gene08628 | PPI |
| MS.gene37499 | MS.gene037122 | PPI |
| MS.gene37499 | MS.gene08876 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37499.t1 | MTR_8g035620 | 95.474 | 1193 | 45 | 3 | 1 | 1184 | 1 | 1193 | 0.0 | 2337 |
| MS.gene37499.t1 | MTR_4g011790 | 22.970 | 505 | 323 | 16 | 146 | 618 | 118 | 588 | 7.83e-19 | 92.4 |
| MS.gene37499.t1 | MTR_4g011790 | 23.031 | 508 | 325 | 16 | 146 | 621 | 118 | 591 | 1.23e-18 | 91.7 |
| MS.gene37499.t1 | MTR_4g011790 | 22.631 | 517 | 325 | 17 | 146 | 621 | 118 | 600 | 4.84e-18 | 90.1 |
| MS.gene37499.t1 | MTR_4g013210 | 21.514 | 502 | 303 | 15 | 146 | 621 | 113 | 549 | 7.76e-18 | 89.0 |
| MS.gene37499.t1 | MTR_2g105570 | 23.540 | 531 | 341 | 17 | 111 | 616 | 46 | 536 | 6.72e-17 | 85.9 |
| MS.gene37499.t1 | MTR_4g013210 | 21.875 | 480 | 313 | 14 | 146 | 593 | 113 | 562 | 6.34e-16 | 83.2 |
| MS.gene37499.t1 | MTR_4g128337 | 22.485 | 507 | 332 | 17 | 130 | 616 | 70 | 535 | 1.88e-15 | 81.6 |
| MS.gene37499.t1 | MTR_1g090737 | 22.457 | 521 | 346 | 13 | 117 | 622 | 82 | 559 | 6.79e-12 | 70.1 |
| MS.gene37499.t1 | MTR_3g460770 | 22.772 | 505 | 315 | 18 | 142 | 621 | 79 | 533 | 8.51e-11 | 66.6 |
| MS.gene37499.t1 | MTR_5g007640 | 23.494 | 498 | 315 | 20 | 142 | 616 | 120 | 574 | 9.46e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37499.t1 | AT5G35930 | 49.958 | 1191 | 498 | 13 | 8 | 1173 | 12 | 1129 | 0.0 | 1130 |
| MS.gene37499.t1 | AT5G35930 | 52.652 | 1056 | 455 | 10 | 143 | 1173 | 2 | 1037 | 0.0 | 1084 |
| MS.gene37499.t1 | AT5G35930 | 51.419 | 916 | 408 | 9 | 283 | 1173 | 1 | 904 | 0.0 | 925 |
| MS.gene37499.t1 | AT5G35930 | 51.419 | 916 | 408 | 9 | 283 | 1173 | 1 | 904 | 0.0 | 925 |
| MS.gene37499.t1 | AT3G16170 | 23.333 | 510 | 321 | 16 | 146 | 624 | 133 | 603 | 3.95e-21 | 99.8 |
| MS.gene37499.t1 | AT1G51680 | 23.800 | 500 | 310 | 17 | 145 | 616 | 92 | 548 | 4.70e-13 | 73.9 |
| MS.gene37499.t1 | AT1G30520 | 21.447 | 387 | 257 | 9 | 258 | 619 | 179 | 543 | 1.08e-12 | 72.8 |
| MS.gene37499.t1 | AT1G30520 | 21.447 | 387 | 257 | 9 | 258 | 619 | 179 | 543 | 1.08e-12 | 72.8 |
| MS.gene37499.t1 | AT1G20510 | 22.539 | 386 | 244 | 10 | 256 | 625 | 194 | 540 | 3.10e-11 | 68.2 |
| MS.gene37499.t1 | AT1G20480 | 21.457 | 494 | 330 | 13 | 142 | 616 | 98 | 552 | 3.41e-11 | 67.8 |
Find 257 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACGATGATTTCTTTATTA+TGG | 0.141918 | 8.3:+60160759 | MS.gene37499:CDS |
| TGCACATGTTGCTCACAATT+TGG | 0.144000 | 8.3:+60160801 | MS.gene37499:CDS |
| TCAAATGTTGATCTCATTAT+AGG | 0.163314 | 8.3:+60155651 | MS.gene37499:CDS |
| CAAAACATCAACTTTATCTT+TGG | 0.189693 | 8.3:-60161016 | None:intergenic |
| TCTTCTGCAACTTATTAAAA+AGG | 0.202078 | 8.3:+60159723 | MS.gene37499:CDS |
| TGGTGGTTCTTGCATATTTA+GGG | 0.217227 | 8.3:+60159177 | MS.gene37499:CDS |
| TCCCTTCTAAGCGCTTAAAA+AGG | 0.228455 | 8.3:+60161040 | MS.gene37499:CDS |
| GTAGAATTGCCTCAAAATTA+TGG | 0.232014 | 8.3:+60159238 | MS.gene37499:CDS |
| TTGGTGGTTCTTGCATATTT+AGG | 0.240857 | 8.3:+60159176 | MS.gene37499:CDS |
| AACTGCAATGTAGTGCTTAT+TGG | 0.245488 | 8.3:+60159118 | MS.gene37499:CDS |
| ATATAAATTCCCACTATCTT+TGG | 0.249934 | 8.3:-60155513 | None:intergenic |
| TGTTATCTGATGTATACTTC+TGG | 0.251820 | 8.3:+60155846 | MS.gene37499:CDS |
| ACTACATTGCAGTTAGTAAT+TGG | 0.256218 | 8.3:-60159109 | None:intergenic |
| AGAACGGTCACCGTGGTAAA+AGG | 0.259018 | 8.3:-60153926 | None:intergenic |
| ATCCTTTCGTTTGGCCAAAA+AGG | 0.259106 | 8.3:-60155609 | None:intergenic |
| ACAACAACTGGGTGAGTTAT+TGG | 0.265149 | 8.3:+60159519 | MS.gene37499:CDS |
| GTGAAGTCTTGGTTGGAGTT+AGG | 0.268803 | 8.3:-60153865 | None:intergenic |
| AGTTAGGGGAGGTGGAATTT+TGG | 0.270321 | 8.3:-60153849 | None:intergenic |
| CATTGGCTGACCAAGTTCTT+TGG | 0.271013 | 8.3:-60161194 | None:intergenic |
| GACGATGATTTCTTTATTAT+GGG | 0.271108 | 8.3:+60160760 | MS.gene37499:CDS |
| CAACGTTGTATTCCCATATT+AGG | 0.271890 | 8.3:-60166170 | None:intergenic |
| CGTCTAAGCTCTATTCTTCA+TGG | 0.276199 | 8.3:+60153994 | MS.gene37499:CDS |
| TGGGGAAGCCTTCTTGCCTT+TGG | 0.281399 | 8.3:+60155584 | MS.gene37499:CDS |
| AGGAAAAGGGAGACCTAATA+TGG | 0.289445 | 8.3:+60166157 | MS.gene37499:intron |
| AGAGCCATACAAAGCTTAAA+AGG | 0.296937 | 8.3:-60160859 | None:intergenic |
| GCACATGTTGCTCACAATTT+GGG | 0.301184 | 8.3:+60160802 | MS.gene37499:CDS |
| GATCGTGCTCTTTGCATAAT+AGG | 0.309303 | 8.3:+60160896 | MS.gene37499:CDS |
| TCATGTGGAGGCAGTATATA+CGG | 0.309480 | 8.3:+60163801 | MS.gene37499:CDS |
| TTCTAAAGTATAATTCGTTT+TGG | 0.312062 | 8.3:-60159285 | None:intergenic |
| TGAAGTCTTGGTTGGAGTTA+GGG | 0.314557 | 8.3:-60153864 | None:intergenic |
| ACATAAGCAGATGCAGTAAT+TGG | 0.315627 | 8.3:-60166198 | None:intergenic |
| TTATAACGAACCTTGAGATT+TGG | 0.322110 | 8.3:-60154043 | None:intergenic |
| AATGAAGATGATGAGAATAT+TGG | 0.322353 | 8.3:-60161117 | None:intergenic |
| ACAAATTCTCAACTTCTTCC+AGG | 0.326568 | 8.3:-60159399 | None:intergenic |
| GATATTTCACGATGCTTTGC+TGG | 0.330880 | 8.3:+60160720 | MS.gene37499:intron |
| ATTTGCGCTTGTTCTAGTTC+TGG | 0.333375 | 8.3:+60166349 | MS.gene37499:CDS |
| TTAGTAATTGGTAAACCTAT+TGG | 0.335883 | 8.3:-60159097 | None:intergenic |
| TGCAGGTTTATTCTGTTAAT+AGG | 0.335925 | 8.3:+60157866 | MS.gene37499:intron |
| AGTTCCCGTGTTTGGTTCTC+TGG | 0.337364 | 8.3:+60164901 | MS.gene37499:CDS |
| GAGGTCACATGAAGAGCTTT+TGG | 0.337985 | 8.3:+60161232 | MS.gene37499:CDS |
| ATCATCCAACTTCTGATTGC+AGG | 0.344242 | 8.3:-60159544 | None:intergenic |
| ATGTAGTTCTTCAACAGATT+TGG | 0.345560 | 8.3:+60155788 | MS.gene37499:CDS |
| AAAACATCAACTTTATCTTT+GGG | 0.352795 | 8.3:-60161015 | None:intergenic |
| ATCCCAACGGATCCATAGTT+TGG | 0.353140 | 8.3:+60165066 | MS.gene37499:CDS |
| TTCTCTTCGCCATTGATGAT+TGG | 0.356998 | 8.3:+60166457 | MS.gene37499:CDS |
| ATTGAAGGACAAACAACAAC+TGG | 0.358221 | 8.3:+60159507 | MS.gene37499:CDS |
| CACGTTGCTTTGGAATTTCA+AGG | 0.358754 | 8.3:-60166528 | None:intergenic |
| ACTTCATACCTTCCAAACTA+TGG | 0.360142 | 8.3:-60165078 | None:intergenic |
| TCAGATTTGTATTTATTCAT+TGG | 0.366412 | 8.3:+60161309 | MS.gene37499:CDS |
| TCAGCTTGAGTTTGCAATAC+AGG | 0.368163 | 8.3:-60157925 | None:intergenic |
| TTCTCTGCATAAATGTTAGA+AGG | 0.370736 | 8.3:+60161349 | MS.gene37499:CDS |
| AAGTTCAAGATTGATGTCTT+TGG | 0.375075 | 8.3:-60158036 | None:intergenic |
| ACATCAATCTTGAACTTATA+CGG | 0.377822 | 8.3:+60158042 | MS.gene37499:CDS |
| TTGCTTTATGTGGCCTCTAC+TGG | 0.378514 | 8.3:+60163947 | MS.gene37499:CDS |
| AAATGAACTTTCAGTATGAT+TGG | 0.379983 | 8.3:-60160971 | None:intergenic |
| CAAATGTTGATCTCATTATA+GGG | 0.383247 | 8.3:+60155652 | MS.gene37499:CDS |
| ATGTATACTTCTGGATCATC+TGG | 0.385691 | 8.3:+60155855 | MS.gene37499:CDS |
| ACAGCAGCATCATTTATATC+TGG | 0.385749 | 8.3:-60159433 | None:intergenic |
| ATGCACGGCCCAGCAAATAT+TGG | 0.386740 | 8.3:-60165196 | None:intergenic |
| ATGTGACCTCTGCTTCCTCT+TGG | 0.387104 | 8.3:-60161219 | None:intergenic |
| TTGGTTGGAGTTAGGGGAGG+TGG | 0.387339 | 8.3:-60153857 | None:intergenic |
| CTTTGGCTTATACTCTTCAA+TGG | 0.391590 | 8.3:-60155496 | None:intergenic |
| AGTAGCCACAGTATACTAAA+TGG | 0.392332 | 8.3:-60164869 | None:intergenic |
| AGAAGTTGAGAATTTGTTAA+GGG | 0.393739 | 8.3:+60159405 | MS.gene37499:CDS |
| ATCGCCGTCATCCACGCTTC+CGG | 0.394712 | 8.3:+60153808 | MS.gene37499:CDS |
| AAATAGAGCTGAGAAATTCT+TGG | 0.400519 | 8.3:-60157629 | None:intergenic |
| TGCAACAAGGTGTTGTATAA+AGG | 0.400733 | 8.3:+60161147 | MS.gene37499:CDS |
| TGAAGCCTTTATAATATTGA+AGG | 0.403942 | 8.3:+60159492 | MS.gene37499:CDS |
| TAAAGTTAATTATGAGTTGT+TGG | 0.407349 | 8.3:+60159639 | MS.gene37499:CDS |
| GTATTTCTGTAGGTGGTTGT+TGG | 0.407551 | 8.3:+60162051 | MS.gene37499:intron |
| TCAATGGAGTATCTTAAAAC+AGG | 0.411099 | 8.3:-60155750 | None:intergenic |
| ATGCTAACAAGTGTTCCAAT+AGG | 0.417652 | 8.3:+60159082 | MS.gene37499:CDS |
| GAATTAGAAGTTCCCGTGTT+TGG | 0.417682 | 8.3:+60164893 | MS.gene37499:CDS |
| TTTGTCCTTCAATATTATAA+AGG | 0.420000 | 8.3:-60159497 | None:intergenic |
| TTCTCTCAGTATTGAGGTGT+GGG | 0.422096 | 8.3:+60155565 | MS.gene37499:CDS |
| ACTGACAGTATCCTTTCGTT+TGG | 0.422407 | 8.3:-60155618 | None:intergenic |
| TTTGTAATCGAGAGCGTATA+AGG | 0.422883 | 8.3:-60163754 | None:intergenic |
| ACTGCTGGTCCAATATTTGC+TGG | 0.422924 | 8.3:+60165187 | MS.gene37499:CDS |
| AACGAATTATACTTTAGAAC+TGG | 0.424139 | 8.3:+60159289 | MS.gene37499:CDS |
| TTCATTTAGTAAAAGTAACT+TGG | 0.433523 | 8.3:+60155683 | MS.gene37499:CDS |
| TCTGAACAGCTCCCTCTTCA+CGG | 0.434497 | 8.3:-60155422 | None:intergenic |
| AAGAAGTTGAGAATTTGTTA+AGG | 0.435328 | 8.3:+60159404 | MS.gene37499:CDS |
| TTATTCTGTTAATAGGCTTA+CGG | 0.437937 | 8.3:+60157873 | MS.gene37499:CDS |
| GGTTCTACAGATGTTACATC+AGG | 0.442750 | 8.3:+60161063 | MS.gene37499:CDS |
| CGCCATTGATGATTGGTGGC+CGG | 0.443792 | 8.3:+60166464 | MS.gene37499:CDS |
| CATTGGCTGGTAAAGTCAAT+TGG | 0.445741 | 8.3:+60155723 | MS.gene37499:CDS |
| CCCTACAACCAACGAAAATC+CGG | 0.446019 | 8.3:-60166483 | None:intergenic |
| TCTGCTTCCTCTTGGAACAT+TGG | 0.446930 | 8.3:-60161211 | None:intergenic |
| CTTGAGATTTGGCTGTGATC+AGG | 0.447470 | 8.3:-60154032 | None:intergenic |
| GTTTGCAAGACTTAAGTTGC+CGG | 0.449434 | 8.3:+60166426 | MS.gene37499:CDS |
| TGTTGACGAGCACTTGCAGC+TGG | 0.453167 | 8.3:+60166218 | MS.gene37499:CDS |
| AACCTTATCCTTGACACTCT+TGG | 0.454137 | 8.3:-60159680 | None:intergenic |
| GGTGGTTGTTGGATGTTACA+TGG | 0.454753 | 8.3:+60162062 | MS.gene37499:intron |
| TAGTAACAAGTTACCTTGTT+CGG | 0.457261 | 8.3:-60155901 | None:intergenic |
| TAAAGGACAAACTTCTGTAA+TGG | 0.458077 | 8.3:+60161164 | MS.gene37499:CDS |
| TCACCCATGGTTGTGTCTAA+AGG | 0.458560 | 8.3:+60161285 | MS.gene37499:CDS |
| GTGCTTGTATGCTGCAGAAA+TGG | 0.463373 | 8.3:+60165584 | MS.gene37499:CDS |
| GACCAAGAGTGTCAAGGATA+AGG | 0.464697 | 8.3:+60159678 | MS.gene37499:CDS |
| AGAGTATAAGCCAAAGATAG+TGG | 0.470385 | 8.3:+60155503 | MS.gene37499:CDS |
| TCTGTAAAGATGAAACGGTT+GGG | 0.471349 | 8.3:-60159592 | None:intergenic |
| TCGGTGCCACACACTCCTTT+AGG | 0.473423 | 8.3:-60155882 | None:intergenic |
| TTTGTTCAGAAAACAACTGC+TGG | 0.473936 | 8.3:+60165172 | MS.gene37499:intron |
| TAATGTTTCATGTAGGTTCG+TGG | 0.475670 | 8.3:+60163923 | MS.gene37499:intron |
| AACAAAATCTCAACTATACA+AGG | 0.476543 | 8.3:-60166561 | None:intergenic |
| GCGGGAAAGGTTGGCGACGC+CGG | 0.479474 | 8.3:-60153827 | None:intergenic |
| GGGGAGATGAACCGTTTCCT+TGG | 0.480295 | 8.3:+60161085 | MS.gene37499:CDS |
| TGTCTGCATGTGTCTACAAC+TGG | 0.481950 | 8.3:-60162650 | None:intergenic |
| TTGCCGTTCTCTCAGTATTG+AGG | 0.487254 | 8.3:+60155559 | MS.gene37499:CDS |
| GACCGTATTGTAAAAGTCCA+TGG | 0.487416 | 8.3:+60159364 | MS.gene37499:CDS |
| TCTTCGCCATTGATGATTGG+TGG | 0.490520 | 8.3:+60166460 | MS.gene37499:CDS |
| TTGGATTAACACCAGGAAAA+GGG | 0.495708 | 8.3:+60166144 | MS.gene37499:intron |
| TGGATACACACCAAAGAACT+TGG | 0.495820 | 8.3:+60161184 | MS.gene37499:CDS |
| GTAATATGTTGCTTGGTAGA+TGG | 0.496013 | 8.3:+60165026 | MS.gene37499:intron |
| CTTCCAAACTATGGATCCGT+TGG | 0.496439 | 8.3:-60165069 | None:intergenic |
| AATGTATCCTTTGACGGGGC+AGG | 0.496532 | 8.3:+60157563 | MS.gene37499:CDS |
| CAAGGAATGTATCCTTTGAC+GGG | 0.497665 | 8.3:+60157558 | MS.gene37499:CDS |
| CTGAAATTGCTTGTGCTAAG+TGG | 0.498055 | 8.3:+60157967 | MS.gene37499:CDS |
| CAAATTCTCAACTTCTTCCA+GGG | 0.501763 | 8.3:-60159398 | None:intergenic |
| GAAGTCTTGGTTGGAGTTAG+GGG | 0.501854 | 8.3:-60153863 | None:intergenic |
| ATGATTCTGTAAAGATGAAA+CGG | 0.503789 | 8.3:-60159597 | None:intergenic |
| AGATGGCCATGTTCTTGCAT+TGG | 0.504157 | 8.3:+60165043 | MS.gene37499:CDS |
| AAATTCACCTCATCAATTGC+AGG | 0.504489 | 8.3:-60163828 | None:intergenic |
| GTGCTCTTTGCATAATAGGT+TGG | 0.507192 | 8.3:+60160900 | MS.gene37499:CDS |
| TTTGGAATGGACACATCTGT+TGG | 0.508610 | 8.3:+60162106 | MS.gene37499:CDS |
| GTAAGAGTGTGGTGAAGTCT+TGG | 0.510548 | 8.3:-60153876 | None:intergenic |
| TTCTGTAAAGATGAAACGGT+TGG | 0.512342 | 8.3:-60159593 | None:intergenic |
| GCAAGGAATGTATCCTTTGA+CGG | 0.512914 | 8.3:+60157557 | MS.gene37499:CDS |
| ATTTCTTCCTTCAAAACCAA+TGG | 0.515072 | 8.3:-60159061 | None:intergenic |
| GTTTAGTGGTTCTATGCAGT+GGG | 0.519242 | 8.3:+60161920 | MS.gene37499:intron |
| GCTTGATGAATCGCATTGGC+TGG | 0.519654 | 8.3:+60155710 | MS.gene37499:CDS |
| GTGGTTGTTGGATGTTACAT+GGG | 0.522213 | 8.3:+60162063 | MS.gene37499:CDS |
| GTTCTTCAACAGATTTGGCC+TGG | 0.523446 | 8.3:+60155793 | MS.gene37499:CDS |
| CACATGTTGCTCACAATTTG+GGG | 0.523615 | 8.3:+60160803 | MS.gene37499:CDS |
| TTTGCAAGACTTAAGTTGCC+GGG | 0.531650 | 8.3:+60166427 | MS.gene37499:CDS |
| AGCTGAGAAATTCTTGGAGG+TGG | 0.531962 | 8.3:-60157623 | None:intergenic |
| TTCCAAACTATGGATCCGTT+GGG | 0.532594 | 8.3:-60165068 | None:intergenic |
| CAGTGGGAGATCAAACTAGA+AGG | 0.532734 | 8.3:+60161936 | MS.gene37499:CDS |
| GCCAATGCGATTCATCAAGC+TGG | 0.532840 | 8.3:-60155707 | None:intergenic |
| AACTTTACCACTAGAAGAGA+CGG | 0.534136 | 8.3:-60159623 | None:intergenic |
| GGATCATCTGGAAAGCCTAA+AGG | 0.534315 | 8.3:+60155867 | MS.gene37499:CDS |
| ATCCGGCCACCAATCATCAA+TGG | 0.535280 | 8.3:-60166466 | None:intergenic |
| GGAAGGGAATGCCAAGGAAA+CGG | 0.535679 | 8.3:-60161096 | None:intergenic |
| GAACGGTCACCGTGGTAAAA+GGG | 0.536923 | 8.3:-60153925 | None:intergenic |
| CCATGGGCAGCGTATTGCCC+TGG | 0.537977 | 8.3:+60159381 | MS.gene37499:CDS |
| GGTTCGTGGATTGCTTTATG+TGG | 0.538084 | 8.3:+60163937 | MS.gene37499:intron |
| GTCTTGGTTGGAGTTAGGGG+AGG | 0.538095 | 8.3:-60153860 | None:intergenic |
| ACACATGCAGACAATTGATC+TGG | 0.539094 | 8.3:+60162660 | MS.gene37499:CDS |
| GGTCACCGTGGTAAAAGGGC+GGG | 0.540768 | 8.3:-60153921 | None:intergenic |
| GGAGTGTGTGGCACCGAACA+AGG | 0.541690 | 8.3:+60155888 | MS.gene37499:CDS |
| GGTTTAGTGGTTCTATGCAG+TGG | 0.546050 | 8.3:+60161919 | MS.gene37499:intron |
| CGGCTGTTCCATCACTGATA+CGG | 0.548026 | 8.3:+60157893 | MS.gene37499:CDS |
| CTGCTGGTCCAATATTTGCT+GGG | 0.548267 | 8.3:+60165188 | MS.gene37499:CDS |
| GGTTGGCGACGCCGGAAGCG+TGG | 0.549572 | 8.3:-60153819 | None:intergenic |
| GATGGTGTCCACCGTGAAGA+GGG | 0.553102 | 8.3:+60155411 | MS.gene37499:CDS |
| ATGCCTCTCACATTTCAGAC+AGG | 0.553275 | 8.3:+60166247 | MS.gene37499:CDS |
| TGGTACCTGCAATCAGAAGT+TGG | 0.554293 | 8.3:+60159539 | MS.gene37499:CDS |
| GTCACCGTGGTAAAAGGGCG+GGG | 0.556780 | 8.3:-60153920 | None:intergenic |
| AGAATTGTCCGTATCAGTGA+TGG | 0.557261 | 8.3:-60157901 | None:intergenic |
| AATAGCTCCTGCCCCGTCAA+AGG | 0.557321 | 8.3:-60157570 | None:intergenic |
| AGCGTCTCCCACAAGGTGAA+AGG | 0.558893 | 8.3:-60158000 | None:intergenic |
| TGCGCTTGTTCTAGTTCTGG+AGG | 0.558942 | 8.3:+60166352 | MS.gene37499:CDS |
| ATCAAAATATGTACAATCGC+CGG | 0.559965 | 8.3:-60159026 | None:intergenic |
| TTGTGTTGATGCTTCACCCA+TGG | 0.560130 | 8.3:+60161272 | MS.gene37499:CDS |
| TGATTGCAAGAGGATACCAT+TGG | 0.561160 | 8.3:+60159045 | MS.gene37499:CDS |
| TTCATTTAGCCGCTGCAACA+AGG | 0.562852 | 8.3:+60161134 | MS.gene37499:CDS |
| ACGTTGCTTTGGAATTTCAA+GGG | 0.564229 | 8.3:-60166527 | None:intergenic |
| GTTGAGAGTAAGTGAAAGAA+CGG | 0.565053 | 8.3:-60153942 | None:intergenic |
| CTGTAAAGATGAAACGGTTG+GGG | 0.566117 | 8.3:-60159591 | None:intergenic |
| AAGATGATGAGAATATTGGA+AGG | 0.566251 | 8.3:-60161113 | None:intergenic |
| AAGTGTGTACTCATTCAAAC+TGG | 0.566453 | 8.3:+60165607 | MS.gene37499:CDS |
| GAGTGTGGTGAAGTCTTGGT+TGG | 0.566749 | 8.3:-60153872 | None:intergenic |
| GTTCATTTCCTCTCGAGTCA+AGG | 0.568276 | 8.3:+60160986 | MS.gene37499:CDS |
| AAAAGGATCCAAAGGCAAGA+AGG | 0.568328 | 8.3:-60155592 | None:intergenic |
| GATGATTTCTTTATTATGGG+TGG | 0.568908 | 8.3:+60160763 | MS.gene37499:CDS |
| TCGTGTGAAATGCAGCAATA+CGG | 0.569742 | 8.3:-60153751 | None:intergenic |
| GTAGTGCTTATTGGTGAAAA+CGG | 0.570737 | 8.3:+60159127 | MS.gene37499:CDS |
| CAACGGATCCATAGTTTGGA+AGG | 0.570864 | 8.3:+60165070 | MS.gene37499:CDS |
| GTTCTCTCAGTATTGAGGTG+TGG | 0.571413 | 8.3:+60155564 | MS.gene37499:CDS |
| TAGAGCTGAGAAATTCTTGG+AGG | 0.571658 | 8.3:-60157626 | None:intergenic |
| ATACGGTGTTTCAGAGGTCA+TGG | 0.572185 | 8.3:-60153734 | None:intergenic |
| GCAATAATATATTCAACAGA+AGG | 0.575476 | 8.3:-60155540 | None:intergenic |
| GGAAAAGGGAGACCTAATAT+GGG | 0.576972 | 8.3:+60166158 | MS.gene37499:intron |
| CGATGGTGTCCACCGTGAAG+AGG | 0.577409 | 8.3:+60155410 | MS.gene37499:CDS |
| TCATTTCCCGTCTCTTCTAG+TGG | 0.577656 | 8.3:+60159616 | MS.gene37499:CDS |
| GGTGGTTCTTGCATATTTAG+GGG | 0.580256 | 8.3:+60159178 | MS.gene37499:CDS |
| CCAGGGCAATACGCTGCCCA+TGG | 0.582484 | 8.3:-60159381 | None:intergenic |
| ATGGCTCTTCTCCATAAAAG+AGG | 0.582942 | 8.3:+60160874 | MS.gene37499:CDS |
| TCTTTGAGCTCGCTGAAAGG+AGG | 0.589038 | 8.3:-60157675 | None:intergenic |
| TTGTGCAGTAATATGTTGCT+TGG | 0.591644 | 8.3:+60165019 | MS.gene37499:intron |
| GAATCTGATATAATGTCTGA+AGG | 0.592170 | 8.3:+60159211 | MS.gene37499:CDS |
| CTTTATGTGGCCTCTACTGG+TGG | 0.592236 | 8.3:+60163950 | MS.gene37499:CDS |
| GGAATCATCCTTGACTCGAG+AGG | 0.592302 | 8.3:-60160994 | None:intergenic |
| AAGAGTGTCAAGGATAAGGT+TGG | 0.593112 | 8.3:+60159682 | MS.gene37499:CDS |
| GACGCCGGAAGCGTGGATGA+CGG | 0.598375 | 8.3:-60153812 | None:intergenic |
| ACTTTACCACTAGAAGAGAC+GGG | 0.598752 | 8.3:-60159622 | None:intergenic |
| GAGTATAAGCCAAAGATAGT+GGG | 0.601040 | 8.3:+60155504 | MS.gene37499:CDS |
| ACCGTATTGTAAAAGTCCAT+GGG | 0.602878 | 8.3:+60159365 | MS.gene37499:CDS |
| CATGTGGAGGCAGTATATAC+GGG | 0.604056 | 8.3:+60163802 | MS.gene37499:CDS |
| AATGTTCCAAGAGGAAGCAG+AGG | 0.604714 | 8.3:+60161213 | MS.gene37499:CDS |
| ATTTGGCTGTGATCAGGTGC+GGG | 0.605125 | 8.3:-60154026 | None:intergenic |
| ATAATATATTCAACAGAAGG+AGG | 0.608760 | 8.3:-60155537 | None:intergenic |
| ACACCTCAATACTGAGAGAA+CGG | 0.611740 | 8.3:-60155562 | None:intergenic |
| CTGATCACAGCCAAATCTCA+AGG | 0.613104 | 8.3:+60154033 | MS.gene37499:CDS |
| GGCGAAGAGAAAATATCCCC+CGG | 0.613716 | 8.3:-60166445 | None:intergenic |
| GACAGCCAGAGAACCAAACA+CGG | 0.614344 | 8.3:-60164906 | None:intergenic |
| TGAAAACGGTGCATCAAATG+AGG | 0.616506 | 8.3:+60159141 | MS.gene37499:CDS |
| ACCAGCTTGATGAATCGCAT+TGG | 0.617253 | 8.3:+60155706 | MS.gene37499:CDS |
| TGGGATCCAATGCAAGAACA+TGG | 0.620941 | 8.3:-60165049 | None:intergenic |
| GGGAGATCAAACTAGAAGGT+CGG | 0.621823 | 8.3:+60161940 | MS.gene37499:CDS |
| ACGAGAACTAAGAGAACGAA+CGG | 0.627734 | 8.3:-60153974 | None:intergenic |
| TATTCTACTGTAGGTAACGA+TGG | 0.629199 | 8.3:+60155393 | MS.gene37499:intron |
| GATTTGGCTGTGATCAGGTG+CGG | 0.629708 | 8.3:-60154027 | None:intergenic |
| AGTTGAAAGCGTCTCCCACA+AGG | 0.630165 | 8.3:-60158007 | None:intergenic |
| AGAGAATAATGTACCTCGTG+AGG | 0.630942 | 8.3:-60165232 | None:intergenic |
| GCAGCAATACGGTGTTTCAG+AGG | 0.630974 | 8.3:-60153740 | None:intergenic |
| GTCTATAAGCTATCATGTGG+AGG | 0.632709 | 8.3:+60163789 | MS.gene37499:CDS |
| CGGGTCACCTGCAATTGATG+AGG | 0.635477 | 8.3:+60163821 | MS.gene37499:CDS |
| CTAATATGGGAATACAACGT+TGG | 0.637142 | 8.3:+60166171 | MS.gene37499:CDS |
| TTGCAAGACTTAAGTTGCCG+GGG | 0.638621 | 8.3:+60166428 | MS.gene37499:CDS |
| GTTCTTGCATTGGATCCCAA+CGG | 0.638673 | 8.3:+60165053 | MS.gene37499:CDS |
| GTTCACCATTTAGTATACTG+TGG | 0.640159 | 8.3:+60164864 | MS.gene37499:CDS |
| AGATGATGAGAATATTGGAA+GGG | 0.641649 | 8.3:-60161112 | None:intergenic |
| GGAAAGCCTAAAGGAGTGTG+TGG | 0.643239 | 8.3:+60155876 | MS.gene37499:CDS |
| TATACAACACCTTGTTGCAG+CGG | 0.644162 | 8.3:-60161143 | None:intergenic |
| AATATTGGAAGGGAATGCCA+AGG | 0.644491 | 8.3:-60161102 | None:intergenic |
| TAACCTGTCTGAAATGTGAG+AGG | 0.645690 | 8.3:-60166250 | None:intergenic |
| GTTCTACAGATGTTACATCA+GGG | 0.645731 | 8.3:+60161064 | MS.gene37499:CDS |
| GAGGGTGAGCTATATGTTGG+TGG | 0.646643 | 8.3:+60159160 | MS.gene37499:CDS |
| CGGTTGGGGAGCAAAACTGA+TGG | 0.647545 | 8.3:-60159577 | None:intergenic |
| ATTATTGACCAAGAGTGTCA+AGG | 0.647880 | 8.3:+60159672 | MS.gene37499:CDS |
| TGGTTGTTGGATGTTACATG+GGG | 0.650491 | 8.3:+60162064 | MS.gene37499:CDS |
| AGTTATACGACCACCAGTAG+AGG | 0.654506 | 8.3:-60163960 | None:intergenic |
| ACCTCGTGAGGATTAACAGA+AGG | 0.654609 | 8.3:-60165220 | None:intergenic |
| TTCTACAGATGTTACATCAG+GGG | 0.654833 | 8.3:+60161065 | MS.gene37499:CDS |
| AAGAATATAAAATCACCACT+TGG | 0.654911 | 8.3:-60159331 | None:intergenic |
| TGCAAGACTTAAGTTGCCGG+GGG | 0.657823 | 8.3:+60166429 | MS.gene37499:CDS |
| TCTACAGATGTTACATCAGG+GGG | 0.659806 | 8.3:+60161066 | MS.gene37499:CDS |
| ACCGTGGTAAAAGGGCGGGG+AGG | 0.660954 | 8.3:-60153917 | None:intergenic |
| GGATTAACAGAAGGTATGCA+CGG | 0.661410 | 8.3:-60165211 | None:intergenic |
| TTGAAGGACAAACAACAACT+GGG | 0.662725 | 8.3:+60159508 | MS.gene37499:CDS |
| TTAGTCAAACAGTTGCCAAG+TGG | 0.664188 | 8.3:+60159316 | MS.gene37499:CDS |
| CGGTCACCGTGGTAAAAGGG+CGG | 0.668056 | 8.3:-60153922 | None:intergenic |
| GAGCCTTTAGACACAACCAT+GGG | 0.668760 | 8.3:-60161288 | None:intergenic |
| TACTGGTGGTCGTATAACTG+CGG | 0.670403 | 8.3:+60163964 | MS.gene37499:CDS |
| AATGAGGGTGAGCTATATGT+TGG | 0.680394 | 8.3:+60159157 | MS.gene37499:CDS |
| TCTCTCAGTATTGAGGTGTG+GGG | 0.689216 | 8.3:+60155566 | MS.gene37499:CDS |
| ACAGCCAGAGAACCAAACAC+GGG | 0.692734 | 8.3:-60164905 | None:intergenic |
| TGGTCAGCCAATGTTCCAAG+AGG | 0.693641 | 8.3:+60161204 | MS.gene37499:CDS |
| TGTGTCTATAAGCTATCATG+TGG | 0.697084 | 8.3:+60163786 | MS.gene37499:CDS |
| GAAAACGGTGCATCAAATGA+GGG | 0.698112 | 8.3:+60159142 | MS.gene37499:CDS |
| TGAGCCTTTAGACACAACCA+TGG | 0.707759 | 8.3:-60161289 | None:intergenic |
| GAACTTATACGGAAGTACCG+AGG | 0.710928 | 8.3:+60158053 | MS.gene37499:CDS |
| ACCTTCTGTTAATCCTCACG+AGG | 0.720615 | 8.3:+60165219 | MS.gene37499:CDS |
| AAGGAATGTATCCTTTGACG+GGG | 0.723446 | 8.3:+60157559 | MS.gene37499:CDS |
| AACGCGCTGCTGTAAGAGTG+TGG | 0.724489 | 8.3:-60153887 | None:intergenic |
| GAACAGCTCCCTCTTCACGG+TGG | 0.729553 | 8.3:-60155419 | None:intergenic |
| AAGTGAAAGAACGGTCACCG+TGG | 0.745920 | 8.3:-60153933 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 60153736 | 60166570 | 60153736 | ID=MS.gene37499 |
| chr8.3 | mRNA | 60153736 | 60166570 | 60153736 | ID=MS.gene37499.t1;Parent=MS.gene37499 |
| chr8.3 | exon | 60153736 | 60154054 | 60153736 | ID=MS.gene37499.t1.exon1;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60153736 | 60154054 | 60153736 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60155406 | 60155909 | 60155406 | ID=MS.gene37499.t1.exon2;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60155406 | 60155909 | 60155406 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60157532 | 60157728 | 60157532 | ID=MS.gene37499.t1.exon3;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60157532 | 60157728 | 60157532 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60157871 | 60158074 | 60157871 | ID=MS.gene37499.t1.exon4;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60157871 | 60158074 | 60157871 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60159022 | 60159744 | 60159022 | ID=MS.gene37499.t1.exon5;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60159022 | 60159744 | 60159022 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60160721 | 60161370 | 60160721 | ID=MS.gene37499.t1.exon6;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60160721 | 60161370 | 60160721 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60161926 | 60161995 | 60161926 | ID=MS.gene37499.t1.exon7;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60161926 | 60161995 | 60161926 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60162063 | 60162149 | 60162063 | ID=MS.gene37499.t1.exon8;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60162063 | 60162149 | 60162063 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60162638 | 60162681 | 60162638 | ID=MS.gene37499.t1.exon9;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60162638 | 60162681 | 60162638 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60163734 | 60163842 | 60163734 | ID=MS.gene37499.t1.exon10;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60163734 | 60163842 | 60163734 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60163938 | 60163997 | 60163938 | ID=MS.gene37499.t1.exon11;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60163938 | 60163997 | 60163938 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60164863 | 60164944 | 60164863 | ID=MS.gene37499.t1.exon12;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60164863 | 60164944 | 60164863 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60165027 | 60165091 | 60165027 | ID=MS.gene37499.t1.exon13;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60165027 | 60165091 | 60165027 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60165181 | 60165240 | 60165181 | ID=MS.gene37499.t1.exon14;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60165181 | 60165240 | 60165181 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60165584 | 60165628 | 60165584 | ID=MS.gene37499.t1.exon15;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60165584 | 60165628 | 60165584 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60166159 | 60166268 | 60166159 | ID=MS.gene37499.t1.exon16;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60166159 | 60166268 | 60166159 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| chr8.3 | exon | 60166345 | 60166570 | 60166345 | ID=MS.gene37499.t1.exon17;Parent=MS.gene37499.t1 |
| chr8.3 | CDS | 60166345 | 60166570 | 60166345 | ID=cds.MS.gene37499.t1;Parent=MS.gene37499.t1 |
| Gene Sequence |
| Protein sequence |
