AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37622

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37622.t1 MTR_5g088230 97.942 243 5 0 1 243 1 243 8.36e-175 482
MS.gene37622.t1 MTR_5g088230 97.899 238 5 0 6 243 1 238 1.49e-170 471
MS.gene37622.t1 MTR_8g043960 44.000 300 117 8 1 252 1 297 2.86e-60 193
MS.gene37622.t1 MTR_3g010660 39.735 302 117 7 1 243 1 296 6.24e-57 184
MS.gene37622.t1 MTR_6g026800 37.838 296 123 8 6 242 1 294 7.46e-34 124
MS.gene37622.t1 MTR_7g088090 43.056 144 63 5 6 140 1 134 1.03e-23 97.8
MS.gene37622.t1 MTR_7g088090 47.619 105 48 3 138 241 194 292 1.91e-15 74.7
MS.gene37622.t1 MTR_3g101780 47.541 122 54 4 22 140 27 141 7.09e-23 96.7
MS.gene37622.t1 MTR_3g101780 42.017 119 63 4 135 247 285 403 1.90e-12 66.6
MS.gene37622.t1 MTR_2g086390 31.174 247 136 8 25 241 11 253 1.24e-21 91.7
MS.gene37622.t1 MTR_2g086390 31.174 247 136 8 25 241 11 253 1.33e-21 91.3
MS.gene37622.t1 MTR_4g085910 40.945 127 60 4 22 140 18 137 3.78e-20 88.6
MS.gene37622.t1 MTR_2g086340 28.819 288 154 8 1 241 1 284 3.88e-16 77.0
MS.gene37622.t1 MTR_7g104480 36.154 130 65 2 29 140 46 175 8.64e-15 73.6
MS.gene37622.t1 MTR_1g098590 36.559 186 83 7 87 245 32 209 4.27e-13 67.0
MS.gene37622.t1 MTR_1g098590 36.559 186 83 7 87 245 32 209 6.41e-13 66.6
MS.gene37622.t1 MTR_1g098590 35.714 196 91 7 76 245 23 209 7.21e-13 66.6
MS.gene37622.t1 MTR_4g094390 37.815 119 53 3 129 245 78 177 2.37e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37622.t1 AT3G56850 42.636 258 110 9 22 243 16 271 3.24e-46 156
MS.gene37622.t1 AT2G41070 41.841 239 108 6 22 245 16 238 1.74e-43 147
MS.gene37622.t1 AT2G41070 40.891 247 114 7 22 252 16 246 2.00e-42 145
MS.gene37622.t1 AT2G41070 40.891 247 114 7 22 252 16 246 2.00e-42 145
MS.gene37622.t1 AT2G41070 40.891 247 114 7 22 252 16 246 2.00e-42 145
MS.gene37622.t1 AT2G41070 40.891 247 114 7 22 252 25 255 3.06e-42 145
MS.gene37622.t1 AT1G49720 35.912 181 96 4 7 171 12 188 4.81e-23 97.1
MS.gene37622.t1 AT1G49720 37.838 111 69 0 135 245 249 359 4.20e-11 62.8
MS.gene37622.t1 AT1G49720 36.747 166 89 3 22 171 23 188 5.32e-23 97.1
MS.gene37622.t1 AT1G49720 37.838 111 69 0 135 245 249 359 4.22e-11 62.8
MS.gene37622.t1 AT1G49720 36.747 166 89 3 22 171 23 188 5.32e-23 97.1
MS.gene37622.t1 AT1G49720 37.838 111 69 0 135 245 249 359 4.22e-11 62.8
MS.gene37622.t1 AT4G34000 39.241 158 57 4 22 140 27 184 1.16e-21 93.6
MS.gene37622.t1 AT4G34000 39.394 99 40 1 146 244 341 419 5.05e-12 65.5
MS.gene37622.t1 AT4G34000 39.241 158 57 4 22 140 27 184 1.81e-21 93.2
MS.gene37622.t1 AT4G34000 39.000 100 41 1 146 245 341 420 4.68e-12 65.9
MS.gene37622.t1 AT4G34000 39.241 158 57 4 22 140 27 184 1.81e-21 93.2
MS.gene37622.t1 AT4G34000 39.000 100 41 1 146 245 341 420 4.68e-12 65.9
MS.gene37622.t1 AT4G34000 39.241 158 57 4 22 140 27 184 1.81e-21 93.2
MS.gene37622.t1 AT4G34000 39.000 100 41 1 146 245 341 420 4.68e-12 65.9
MS.gene37622.t1 AT3G19290 38.686 137 66 3 22 140 34 170 4.54e-20 89.0
MS.gene37622.t1 AT3G19290 38.686 137 66 3 22 140 34 170 5.73e-20 88.6
MS.gene37622.t1 AT3G19290 45.679 81 43 1 165 245 320 399 2.21e-11 63.5
MS.gene37622.t1 AT3G19290 38.686 137 66 3 22 140 34 170 8.73e-20 88.2
MS.gene37622.t1 AT3G19290 45.679 81 43 1 165 245 320 399 3.43e-11 63.2
MS.gene37622.t1 AT3G19290 38.686 137 66 3 22 140 34 170 9.12e-20 88.2
MS.gene37622.t1 AT3G19290 45.679 81 43 1 165 245 320 399 3.42e-11 63.2
MS.gene37622.t1 AT3G19290 38.686 137 66 3 22 140 34 170 9.12e-20 88.2
MS.gene37622.t1 AT3G19290 45.679 81 43 1 165 245 320 399 3.42e-11 63.2
MS.gene37622.t1 AT3G44460 33.721 258 128 6 24 241 37 291 1.04e-19 87.0
MS.gene37622.t1 AT1G03970 40.123 162 72 5 94 242 83 232 4.83e-18 81.6
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.21e-17 79.7
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.21e-17 79.7
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.21e-17 79.7
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.21e-17 79.7
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.34e-17 79.7
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.34e-17 79.7
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 6.79e-17 79.7
MS.gene37622.t1 AT1G45249 42.202 109 55 3 142 245 279 384 5.67e-12 65.5
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 36 165 7.80e-17 79.7
MS.gene37622.t1 AT1G45249 42.202 109 55 3 142 245 294 399 6.76e-12 65.1
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 36 165 7.80e-17 79.7
MS.gene37622.t1 AT1G45249 42.202 109 55 3 142 245 294 399 6.76e-12 65.1
MS.gene37622.t1 AT1G45249 38.849 139 56 5 22 140 21 150 1.02e-16 79.3
MS.gene37622.t1 AT5G44080 34.254 181 84 7 96 250 120 291 8.71e-14 70.1
MS.gene37622.t1 AT2G17770 29.480 173 96 5 98 245 1 172 8.27e-13 65.9
MS.gene37622.t1 AT2G36270 50.000 78 29 1 184 251 342 419 2.89e-12 66.2
MS.gene37622.t1 AT2G36270 61.404 57 22 0 184 240 342 398 5.81e-12 65.5
MS.gene37622.t1 AT2G36270 61.404 57 22 0 184 240 342 398 5.81e-12 65.5
MS.gene37622.t1 AT4G35900 50.000 78 30 2 168 245 194 262 6.29e-11 61.6

Find 71 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGTCTACTAGGAGATTT+GGG 0.275351 5.3:+70861274 MS.gene37622:CDS
TAATAGTCTACTAGGAGATT+TGG 0.281034 5.3:+70861273 MS.gene37622:CDS
CCTTGGTAACCAGTTCATTA+TGG 0.284719 5.3:-70861944 None:intergenic
TGGGCAACAGTCTCATTTAC+AGG 0.354570 5.3:+70861198 MS.gene37622:CDS
GCTGAAGGAAATAAGGTTGT+AGG 0.386567 5.3:+70861349 MS.gene37622:CDS
GGAGATTTGGGGAAGCCGTT+AGG 0.390252 5.3:+70861286 MS.gene37622:CDS
TGAGAAGGAAAATCAAGAAT+AGG 0.395993 5.3:+70861767 MS.gene37622:CDS
GTATTCGGTTGAAAGCTTAG+AGG 0.400524 5.3:-70861643 None:intergenic
GAGGATACCAAGCTTGTATT+CGG 0.402791 5.3:-70861658 None:intergenic
CAATGGGTTCTAAAAGTAAT+GGG 0.403194 5.3:+70861179 MS.gene37622:CDS
ATGCAAAACCAGGTCGCAAA+AGG 0.411790 5.3:+70861704 MS.gene37622:CDS
AGTTAGTTAAGCAAAATTCA+TGG 0.414879 5.3:+70861227 MS.gene37622:CDS
ATTGTTTCTTCTGACCTAGC+TGG 0.422038 5.3:-70861480 None:intergenic
GAGAAGGAAAATCAAGAATA+GGG 0.428756 5.3:+70861768 MS.gene37622:CDS
AACAAACAGCAAACACTAAG+TGG 0.429875 5.3:-70862036 None:intergenic
GCGTTCACGTGCAAGAAAAC+AGG 0.432338 5.3:+70861801 MS.gene37622:CDS
GTGGACTGCTGAAGGAAATA+AGG 0.439159 5.3:+70861342 MS.gene37622:CDS
CTAGGTCAGAAGAAACAATA+TGG 0.453967 5.3:+70861484 MS.gene37622:CDS
GAGATTTGGGGAAGCCGTTA+GGG 0.461727 5.3:+70861287 MS.gene37622:CDS
GGATGCATATGAGAAGGCTT+TGG 0.465775 5.3:+70861735 MS.gene37622:CDS
ACAATGGGTTCTAAAAGTAA+TGG 0.467214 5.3:+70861178 MS.gene37622:CDS
GTTAAGCAAAATTCATGGTA+TGG 0.470540 5.3:+70861232 MS.gene37622:CDS
GGAAGACGGTGGATGATGTA+TGG 0.489215 5.3:+70861449 MS.gene37622:CDS
TATTAGTCCGACGGTGGGGT+TGG 0.490449 5.3:+70861600 MS.gene37622:CDS
GAGTCTCGGTGGTACGACTT+TGG 0.502186 5.3:+70861537 MS.gene37622:CDS
ATCGTCTTCTCTTCAACGGC+AGG 0.510272 5.3:+70861396 MS.gene37622:CDS
GCTTCTATTAGTCCGACGGT+GGG 0.511307 5.3:+70861595 MS.gene37622:CDS
GACGGTGGGGTTGGATGCAA+TGG 0.513361 5.3:+70861609 MS.gene37622:CDS
AGGTCGCAAAAGGGATGCTC+CGG 0.515870 5.3:+70861714 MS.gene37622:CDS
CTGAAGGAAATAAGGTTGTA+GGG 0.518539 5.3:+70861350 MS.gene37622:CDS
GCAGGCTTACCATAATGAAC+TGG 0.521206 5.3:+70861935 MS.gene37622:intron
AGGAAATAAGGTTGTAGGGA+TGG 0.523139 5.3:+70861354 MS.gene37622:CDS
CTTTGGAAAGGAGGCTGAGA+AGG 0.527338 5.3:+70861752 MS.gene37622:CDS
CTTTCAACCGAATACAAGCT+TGG 0.528662 5.3:+70861651 MS.gene37622:CDS
CAAACTACTAATTGAAGAAG+AGG 0.529048 5.3:-70861677 None:intergenic
TTGAATGGGGATTCAAACAA+TGG 0.534421 5.3:+70861162 None:intergenic
TGAATGGGGATTCAAACAAT+GGG 0.535901 5.3:+70861163 None:intergenic
AAAGCCTTCTCATATGCATC+CGG 0.537373 5.3:-70861733 None:intergenic
TTGTTCTAGCAGAGTAACCT+TGG 0.544023 5.3:-70861961 None:intergenic
GATGAGGTTAATAGTCTACT+AGG 0.549320 5.3:+70861265 MS.gene37622:CDS
TGTAGGGATGGAGAGTGAGC+AGG 0.552172 5.3:+70861366 MS.gene37622:CDS
CGTCATCGTCTTCTCTTCAA+CGG 0.553592 5.3:+70861392 MS.gene37622:CDS
CAGAATGTGTGGACTGCTGA+AGG 0.557306 5.3:+70861334 MS.gene37622:CDS
ACAAACAGCAAACACTAAGT+GGG 0.566411 5.3:-70862035 None:intergenic
TGCAAAACCAGGTCGCAAAA+GGG 0.571670 5.3:+70861705 MS.gene37622:CDS
ATGAGAAGGCTTTGGAAAGG+AGG 0.572932 5.3:+70861743 MS.gene37622:CDS
TGGAGCTTCTATTAGTCCGA+CGG 0.576977 5.3:+70861591 MS.gene37622:CDS
AGCTTCTATTAGTCCGACGG+TGG 0.577860 5.3:+70861594 MS.gene37622:CDS
ACGAGCTTCTTCAGAATGTG+TGG 0.579972 5.3:+70861323 MS.gene37622:CDS
CGTTGGCTCGTGCTTTGAGT+GGG 0.589340 5.3:+70861428 MS.gene37622:CDS
TCGTGCTTTGAGTGGGAAGA+CGG 0.590102 5.3:+70861435 MS.gene37622:CDS
ACGTTGGCTCGTGCTTTGAG+TGG 0.590747 5.3:+70861427 MS.gene37622:CDS
AGTTTGTCAGATGCAAAACC+AGG 0.591285 5.3:+70861694 MS.gene37622:CDS
TCAAGATTCATGCTCCCTAA+CGG 0.602234 5.3:-70861301 None:intergenic
CCATAATGAACTGGTTACCA+AGG 0.615234 5.3:+70861944 MS.gene37622:CDS
GTATGGTCTAACTCTTGATG+AGG 0.618117 5.3:+70861249 MS.gene37622:CDS
CTTCTATTAGTCCGACGGTG+GGG 0.618855 5.3:+70861596 MS.gene37622:CDS
TGGCGACGATGTGAAAGTTG+AGG 0.621646 5.3:+70861504 MS.gene37622:CDS
CAAACAGCAAACACTAAGTG+GGG 0.632691 5.3:-70862034 None:intergenic
TGCTCCGGATGCATATGAGA+AGG 0.633586 5.3:+70861729 MS.gene37622:CDS
TATGCAGCTCAAGAAAGAGA+AGG 0.635674 5.3:+70861989 MS.gene37622:CDS
GAGGATAGCGAAATGAGTCT+CGG 0.636669 5.3:+70861523 MS.gene37622:CDS
TGCTTTGAGTGGGAAGACGG+TGG 0.649656 5.3:+70861438 MS.gene37622:CDS
ATTGCATCCAACCCCACCGT+CGG 0.650426 5.3:-70861607 None:intergenic
CATATGAGAAGGCTTTGGAA+AGG 0.652484 5.3:+70861740 MS.gene37622:CDS
GTATGGAGAGAAATCCAGCT+AGG 0.652627 5.3:+70861466 MS.gene37622:CDS
TTGAAAGCTTAGAGGAATAG+CGG 0.661783 5.3:-70861635 None:intergenic
ACGGCAGGCTAGTATGACGT+TGG 0.673052 5.3:+70861411 MS.gene37622:CDS
ATAGTCTACTAGGAGATTTG+GGG 0.688137 5.3:+70861275 MS.gene37622:CDS
AAACAGCAAACACTAAGTGG+GGG 0.698048 5.3:-70862033 None:intergenic
GATAGCGAAATGAGTCTCGG+TGG 0.714876 5.3:+70861526 MS.gene37622:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAATATATTCCAACTA+AGG - chr5.3:70861893-70861912 None:intergenic 20.0%
! AGTTAGTTAAGCAAAATTCA+TGG + chr5.3:70861227-70861246 MS.gene37622:CDS 25.0%
! TAAGGAAAATGATGAATAGA+CGG - chr5.3:70861875-70861894 None:intergenic 25.0%
! TTACTTTACATAACGCATAA+AGG - chr5.3:70861832-70861851 None:intergenic 25.0%
!! TATTCATCATTTTCCTTAGT+TGG + chr5.3:70861877-70861896 MS.gene37622:intron 25.0%
!! TTTGTCACTTAAAAGTTTGA+TGG + chr5.3:70861911-70861930 MS.gene37622:intron 25.0%
AATAGTCTACTAGGAGATTT+GGG + chr5.3:70861274-70861293 MS.gene37622:CDS 30.0%
CAAACTACTAATTGAAGAAG+AGG - chr5.3:70861680-70861699 None:intergenic 30.0%
GAGAAGGAAAATCAAGAATA+GGG + chr5.3:70861768-70861787 MS.gene37622:CDS 30.0%
GTTAAGCAAAATTCATGGTA+TGG + chr5.3:70861232-70861251 MS.gene37622:CDS 30.0%
TAATAGTCTACTAGGAGATT+TGG + chr5.3:70861273-70861292 MS.gene37622:CDS 30.0%
TGAGAAGGAAAATCAAGAAT+AGG + chr5.3:70861767-70861786 MS.gene37622:CDS 30.0%
!! ACAATGGGTTCTAAAAGTAA+TGG + chr5.3:70861178-70861197 MS.gene37622:CDS 30.0%
!! CAATGGGTTCTAAAAGTAAT+GGG + chr5.3:70861179-70861198 MS.gene37622:CDS 30.0%
!!! AAGATTTTTTAGTGCAAGCA+GGG + chr5.3:70861560-70861579 MS.gene37622:CDS 30.0%
ATAGTCTACTAGGAGATTTG+GGG + chr5.3:70861275-70861294 MS.gene37622:CDS 35.0%
CTAGGTCAGAAGAAACAATA+TGG + chr5.3:70861484-70861503 MS.gene37622:CDS 35.0%
CTGAAGGAAATAAGGTTGTA+GGG + chr5.3:70861350-70861369 MS.gene37622:CDS 35.0%
GATGAGGTTAATAGTCTACT+AGG + chr5.3:70861265-70861284 MS.gene37622:CDS 35.0%
TTGAAAGCTTAGAGGAATAG+CGG - chr5.3:70861638-70861657 None:intergenic 35.0%
!! ACTTAAAAGTTTGATGGTGC+AGG + chr5.3:70861917-70861936 MS.gene37622:intron 35.0%
!!! GAAGATTTTTTAGTGCAAGC+AGG + chr5.3:70861559-70861578 MS.gene37622:CDS 35.0%
AAAGCCTTCTCATATGCATC+CGG - chr5.3:70861736-70861755 None:intergenic 40.0%
AGGAAATAAGGTTGTAGGGA+TGG + chr5.3:70861354-70861373 MS.gene37622:CDS 40.0%
AGTTTGTCAGATGCAAAACC+AGG + chr5.3:70861694-70861713 MS.gene37622:CDS 40.0%
CCATAATGAACTGGTTACCA+AGG + chr5.3:70861944-70861963 MS.gene37622:CDS 40.0%
CTTTCAACCGAATACAAGCT+TGG + chr5.3:70861651-70861670 MS.gene37622:CDS 40.0%
GAGGATACCAAGCTTGTATT+CGG - chr5.3:70861661-70861680 None:intergenic 40.0%
GCTGAAGGAAATAAGGTTGT+AGG + chr5.3:70861349-70861368 MS.gene37622:CDS 40.0%
GTATGGTCTAACTCTTGATG+AGG + chr5.3:70861249-70861268 MS.gene37622:CDS 40.0%
GTATTCGGTTGAAAGCTTAG+AGG - chr5.3:70861646-70861665 None:intergenic 40.0%
TATGCAGCTCAAGAAAGAGA+AGG + chr5.3:70861989-70862008 MS.gene37622:CDS 40.0%
TCAAGATTCATGCTCCCTAA+CGG - chr5.3:70861304-70861323 None:intergenic 40.0%
TTGTTCTAGCAGAGTAACCT+TGG - chr5.3:70861964-70861983 None:intergenic 40.0%
! ATTGTTTCTTCTGACCTAGC+TGG - chr5.3:70861483-70861502 None:intergenic 40.0%
! CCTTGGTAACCAGTTCATTA+TGG - chr5.3:70861947-70861966 None:intergenic 40.0%
!! CATATGAGAAGGCTTTGGAA+AGG + chr5.3:70861740-70861759 MS.gene37622:CDS 40.0%
ACGAGCTTCTTCAGAATGTG+TGG + chr5.3:70861323-70861342 MS.gene37622:CDS 45.0%
ATGCAAAACCAGGTCGCAAA+AGG + chr5.3:70861704-70861723 MS.gene37622:CDS 45.0%
CACGTGCAAGAAAACAGGTT+TGG + chr5.3:70861806-70861825 MS.gene37622:intron 45.0%
CGTCATCGTCTTCTCTTCAA+CGG + chr5.3:70861392-70861411 MS.gene37622:CDS 45.0%
GAGGATAGCGAAATGAGTCT+CGG + chr5.3:70861523-70861542 MS.gene37622:CDS 45.0%
GCAGGCTTACCATAATGAAC+TGG + chr5.3:70861935-70861954 MS.gene37622:intron 45.0%
GGATGCATATGAGAAGGCTT+TGG + chr5.3:70861735-70861754 MS.gene37622:CDS 45.0%
GTATGGAGAGAAATCCAGCT+AGG + chr5.3:70861466-70861485 MS.gene37622:CDS 45.0%
TGCAAAACCAGGTCGCAAAA+GGG + chr5.3:70861705-70861724 MS.gene37622:CDS 45.0%
TGGAGCTTCTATTAGTCCGA+CGG + chr5.3:70861591-70861610 MS.gene37622:CDS 45.0%
TGGGCAACAGTCTCATTTAC+AGG + chr5.3:70861198-70861217 MS.gene37622:CDS 45.0%
! GTGGACTGCTGAAGGAAATA+AGG + chr5.3:70861342-70861361 MS.gene37622:CDS 45.0%
!! ATGAGAAGGCTTTGGAAAGG+AGG + chr5.3:70861743-70861762 MS.gene37622:CDS 45.0%
AGCTTCTATTAGTCCGACGG+TGG + chr5.3:70861594-70861613 MS.gene37622:CDS 50.0%
ATCGTCTTCTCTTCAACGGC+AGG + chr5.3:70861396-70861415 MS.gene37622:CDS 50.0%
CTTCTATTAGTCCGACGGTG+GGG + chr5.3:70861596-70861615 MS.gene37622:CDS 50.0%
GAGATTTGGGGAAGCCGTTA+GGG + chr5.3:70861287-70861306 MS.gene37622:CDS 50.0%
GATAGCGAAATGAGTCTCGG+TGG + chr5.3:70861526-70861545 MS.gene37622:CDS 50.0%
GCGTTCACGTGCAAGAAAAC+AGG + chr5.3:70861801-70861820 MS.gene37622:CDS 50.0%
GCTTCTATTAGTCCGACGGT+GGG + chr5.3:70861595-70861614 MS.gene37622:CDS 50.0%
GGAAGACGGTGGATGATGTA+TGG + chr5.3:70861449-70861468 MS.gene37622:CDS 50.0%
TGCTCCGGATGCATATGAGA+AGG + chr5.3:70861729-70861748 MS.gene37622:CDS 50.0%
TGGCGACGATGTGAAAGTTG+AGG + chr5.3:70861504-70861523 MS.gene37622:CDS 50.0%
! CAGAATGTGTGGACTGCTGA+AGG + chr5.3:70861334-70861353 MS.gene37622:CDS 50.0%
! CTTTGGAAAGGAGGCTGAGA+AGG + chr5.3:70861752-70861771 MS.gene37622:CDS 50.0%
! TCGTGCTTTGAGTGGGAAGA+CGG + chr5.3:70861435-70861454 MS.gene37622:CDS 50.0%
! TTTTCTTGCACGTGAACGCG+CGG - chr5.3:70861800-70861819 None:intergenic 50.0%
ACGGCAGGCTAGTATGACGT+TGG + chr5.3:70861411-70861430 MS.gene37622:CDS 55.0%
AGGTCGCAAAAGGGATGCTC+CGG + chr5.3:70861714-70861733 MS.gene37622:CDS 55.0%
ATTGCATCCAACCCCACCGT+CGG - chr5.3:70861610-70861629 None:intergenic 55.0%
GAGTCTCGGTGGTACGACTT+TGG + chr5.3:70861537-70861556 MS.gene37622:CDS 55.0%
GGAGATTTGGGGAAGCCGTT+AGG + chr5.3:70861286-70861305 MS.gene37622:CDS 55.0%
TATTAGTCCGACGGTGGGGT+TGG + chr5.3:70861600-70861619 MS.gene37622:CDS 55.0%
TGTAGGGATGGAGAGTGAGC+AGG + chr5.3:70861366-70861385 MS.gene37622:CDS 55.0%
! GTGCAAGCAGGGCTTTTTGC+TGG + chr5.3:70861571-70861590 MS.gene37622:CDS 55.0%
! TGCTTTGAGTGGGAAGACGG+TGG + chr5.3:70861438-70861457 MS.gene37622:CDS 55.0%
!! ACGTTGGCTCGTGCTTTGAG+TGG + chr5.3:70861427-70861446 MS.gene37622:CDS 55.0%
!! CGTTGGCTCGTGCTTTGAGT+GGG + chr5.3:70861428-70861447 MS.gene37622:CDS 55.0%
GACGGTGGGGTTGGATGCAA+TGG + chr5.3:70861609-70861628 MS.gene37622:CDS 60.0%
!! GGAGCATCCCTTTTGCGACC+TGG - chr5.3:70861715-70861734 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 70861166 70862043 70861166 ID=MS.gene37622
chr5.3 mRNA 70861166 70862043 70861166 ID=MS.gene37622.t1;Parent=MS.gene37622
chr5.3 exon 70861166 70861822 70861166 ID=MS.gene37622.t1.exon1;Parent=MS.gene37622.t1
chr5.3 CDS 70861166 70861822 70861166 ID=cds.MS.gene37622.t1;Parent=MS.gene37622.t1
chr5.3 exon 70861939 70862043 70861939 ID=MS.gene37622.t1.exon2;Parent=MS.gene37622.t1
chr5.3 CDS 70861939 70862043 70861939 ID=cds.MS.gene37622.t1;Parent=MS.gene37622.t1
Gene Sequence

>MS.gene37622

ATGGGGATTCAAACAATGGGTTCTAAAAGTAATGGGCAACAGTCTCATTTACAGGCTAATCAGTTAGTTAAGCAAAATTCATGGTATGGTCTAACTCTTGATGAGGTTAATAGTCTACTAGGAGATTTGGGGAAGCCGTTAGGGAGCATGAATCTTGACGAGCTTCTTCAGAATGTGTGGACTGCTGAAGGAAATAAGGTTGTAGGGATGGAGAGTGAGCAGGCTTCGTCATCGTCTTCTCTTCAACGGCAGGCTAGTATGACGTTGGCTCGTGCTTTGAGTGGGAAGACGGTGGATGATGTATGGAGAGAAATCCAGCTAGGTCAGAAGAAACAATATGGCGACGATGTGAAAGTTGAGGATAGCGAAATGAGTCTCGGTGGTACGACTTTGGAAGATTTTTTAGTGCAAGCAGGGCTTTTTGCTGGAGCTTCTATTAGTCCGACGGTGGGGTTGGATGCAATGGATACCGCTATTCCTCTAAGCTTTCAACCGAATACAAGCTTGGTATCCTCTTCTTCAATTAGTAGTTTGTCAGATGCAAAACCAGGTCGCAAAAGGGATGCTCCGGATGCATATGAGAAGGCTTTGGAAAGGAGGCTGAGAAGGAAAATCAAGAATAGGGAATCTGCCGCGCGTTCACGTGCAAGAAAACAGGTTTGGCCTTTATGCGTTATGTAAAGTAATAAGCTTAAATTGATTGTATCCGTCTATTCATCATTTTCCTTAGTTGGAATATATTTGTTTTGTCACTTAAAAGTTTGATGGTGCAGGCTTACCATAATGAACTGGTTACCAAGGTTACTCTGCTAGAACAACAAAATATGCAGCTCAAGAAAGAGAAGGTGCAAGATCTCATTCATTTTTCCCCCACTTAG
Protein sequence

>MS.gene37622.t1

MGIQTMGSKSNGQQSHLQANQLVKQNSWYGLTLDEVNSLLGDLGKPLGSMNLDELLQNVWTAEGNKVVGMESEQASSSSSLQRQASMTLARALSGKTVDDVWREIQLGQKKQYGDDVKVEDSEMSLGGTTLEDFLVQAGLFAGASISPTVGLDAMDTAIPLSFQPNTSLVSSSSISSLSDAKPGRKRDAPDAYEKALERRLRRKIKNRESAARSRARKQAYHNELVTKVTLLEQQNMQLKKEKVQDLIHFSPT