Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38022.t1 | XP_003617922.1 | 99.6 | 473 | 2 | 0 | 1 | 473 | 1 | 473 | 5.70E-268 | 932.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38022.t1 | Q9ZW34 | 82.4 | 472 | 83 | 0 | 1 | 472 | 1 | 472 | 4.9e-228 | 791.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38022.t1 | G7K4W1 | 99.6 | 473 | 2 | 0 | 1 | 473 | 1 | 473 | 4.1e-268 | 932.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045798 | MS.gene38022 | PPI |
| MS.gene38022 | MS.gene78187 | PPI |
| MS.gene83234 | MS.gene38022 | PPI |
| MS.gene020556 | MS.gene38022 | PPI |
| MS.gene007304 | MS.gene38022 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38022.t1 | MTR_5g096970 | 99.577 | 473 | 2 | 0 | 1 | 473 | 1 | 473 | 0.0 | 970 |
| MS.gene38022.t1 | MTR_7g103620 | 59.361 | 438 | 167 | 7 | 45 | 472 | 2 | 438 | 1.37e-180 | 513 |
| MS.gene38022.t1 | MTR_2g056190 | 58.605 | 430 | 175 | 3 | 44 | 472 | 62 | 489 | 1.73e-177 | 506 |
| MS.gene38022.t1 | MTR_6g060570 | 56.979 | 437 | 159 | 8 | 47 | 472 | 5 | 423 | 2.11e-165 | 474 |
| MS.gene38022.t1 | MTR_7g103620 | 58.713 | 373 | 145 | 5 | 109 | 472 | 16 | 388 | 7.10e-152 | 438 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38022.t1 | AT2G29560 | 82.415 | 472 | 83 | 0 | 1 | 472 | 1 | 472 | 0.0 | 820 |
| MS.gene38022.t1 | AT2G36530 | 58.998 | 439 | 169 | 7 | 45 | 473 | 2 | 439 | 3.33e-179 | 509 |
| MS.gene38022.t1 | AT1G74030 | 58.503 | 441 | 173 | 5 | 40 | 472 | 37 | 475 | 1.25e-177 | 506 |
| MS.gene38022.t1 | AT2G36530 | 58.134 | 418 | 164 | 7 | 66 | 473 | 12 | 428 | 4.59e-167 | 478 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACGGGGTCGGAGGTTT+TGG | 0.248202 | 5.1:-77346206 | None:intergenic |
| TGAGACCTATCATCATTTAA+AGG | 0.262267 | 5.1:+77350101 | MS.gene38022:CDS |
| GCAGGCAAAGGAAGCGCATT+GGG | 0.283596 | 5.1:+77355362 | MS.gene38022:CDS |
| CCTTGATAAAACAGAGAAAA+AGG | 0.306249 | 5.1:+77347722 | MS.gene38022:CDS |
| GGATCTTCTATTGATACAAT+TGG | 0.313466 | 5.1:-77354076 | None:intergenic |
| TTGTAATGCTCTTCTTCTTA+AGG | 0.320244 | 5.1:+77354826 | MS.gene38022:CDS |
| GGCTCTGACGGCGGCATTGA+CGG | 0.326530 | 5.1:-77346185 | None:intergenic |
| TGACGGCGTCTTCGAGTTTA+CGG | 0.335239 | 5.1:-77346168 | None:intergenic |
| TCAAATCTGCTTGCTCCAAT+TGG | 0.357446 | 5.1:-77350057 | None:intergenic |
| ATCCATTTGACAAGGAAGAT+TGG | 0.376004 | 5.1:+77354095 | MS.gene38022:CDS |
| TCCATTGCTGCATGTAAAGC+TGG | 0.377107 | 5.1:+77348662 | MS.gene38022:CDS |
| TGAATGATTCTGCAGGTAGT+AGG | 0.386036 | 5.1:+77354746 | MS.gene38022:intron |
| GTATGAGGCTGTTGAGTTAC+GGG | 0.389583 | 5.1:+77347569 | MS.gene38022:CDS |
| ATCATAACCGGTTCTACTAA+TGG | 0.389720 | 5.1:-77352521 | None:intergenic |
| CAGTTTATGCTGGTGAAGAT+TGG | 0.398772 | 5.1:+77356194 | MS.gene38022:CDS |
| TTTCGTGCTTCTGTTCCATC+TGG | 0.400465 | 5.1:+77346444 | MS.gene38022:CDS |
| CTATCATAGCATGATCAATC+TGG | 0.408498 | 5.1:-77347698 | None:intergenic |
| TCCATTTGACAAGGAAGATT+GGG | 0.410260 | 5.1:+77354096 | MS.gene38022:CDS |
| AAATTCTGTCCCGACGTTTG+AGG | 0.413766 | 5.1:-77353902 | None:intergenic |
| GATGATTCATTTCAGGTATG+AGG | 0.417338 | 5.1:+77347554 | MS.gene38022:intron |
| AAGCACATCGCTGACCTGTC+TGG | 0.418545 | 5.1:+77348819 | MS.gene38022:CDS |
| TCCCAATCTTCCTTGTCAAA+TGG | 0.418960 | 5.1:-77354097 | None:intergenic |
| GGTATGAGGCTGTTGAGTTA+CGG | 0.423365 | 5.1:+77347568 | MS.gene38022:intron |
| TGGCCTCCTTTACAAGATCC+AGG | 0.431446 | 5.1:-77352501 | None:intergenic |
| TTCATAGCTGATTTATCCGT+TGG | 0.433997 | 5.1:+77355426 | MS.gene38022:CDS |
| AAAGCTTGCCTGGAGACATT+AGG | 0.436617 | 5.1:-77351516 | None:intergenic |
| AAAACATGCTGGAAATAATC+TGG | 0.443130 | 5.1:+77348893 | MS.gene38022:CDS |
| AATCACCTTTAAATGATGAT+AGG | 0.445742 | 5.1:-77350106 | None:intergenic |
| GATATTACGATGAAGAGAAC+GGG | 0.448791 | 5.1:-77346220 | None:intergenic |
| GAAATCATGATTCTTCCAAT+TGG | 0.450349 | 5.1:+77350042 | MS.gene38022:CDS |
| GATCTTCTATTGATACAATT+GGG | 0.456790 | 5.1:-77354075 | None:intergenic |
| TAAAGCTGGGGCAGCTGAAA+AGG | 0.459847 | 5.1:+77348676 | MS.gene38022:CDS |
| CTTCGGATTGAGGAAGAGCT+TGG | 0.459854 | 5.1:+77356163 | MS.gene38022:CDS |
| CTCAAGCCTTGGAATTTGCC+AGG | 0.463723 | 5.1:+77354135 | MS.gene38022:CDS |
| GAATGATTCTGCAGGTAGTA+GGG | 0.464232 | 5.1:+77354747 | MS.gene38022:intron |
| GGGGACAAAGGTGTATATCT+AGG | 0.471083 | 5.1:+77347594 | MS.gene38022:CDS |
| ATCAAGTATATCTCAAGCCT+TGG | 0.478687 | 5.1:+77354124 | MS.gene38022:CDS |
| CACCAGCTTTAGAGAAGCCC+TGG | 0.479064 | 5.1:+77352483 | MS.gene38022:intron |
| AAGGAGGCCATTAGTAGAAC+CGG | 0.479320 | 5.1:+77352514 | MS.gene38022:CDS |
| GACGGCGTCTTCGAGTTTAC+GGG | 0.479590 | 5.1:-77346167 | None:intergenic |
| AACAGGTAAGAGATATGATC+TGG | 0.481488 | 5.1:+77353868 | MS.gene38022:intron |
| AAAATATCTGAGGCGCTGAT+TGG | 0.482231 | 5.1:+77347654 | MS.gene38022:CDS |
| TGGAAATAATCTGGCAATTG+AGG | 0.486178 | 5.1:+77348902 | MS.gene38022:CDS |
| AAAGCAGGAACAGGAAGCAC+TGG | 0.490991 | 5.1:-77348852 | None:intergenic |
| CCTCGGTAACAGTTCCAACT+TGG | 0.491628 | 5.1:-77355323 | None:intergenic |
| GATACAGTTACTTCGGATTG+AGG | 0.493277 | 5.1:+77356153 | MS.gene38022:intron |
| GGCTGTTGAGTTACGGGATG+GGG | 0.493888 | 5.1:+77347575 | MS.gene38022:CDS |
| ATAACAGTAAAAGCAGGAAC+AGG | 0.498354 | 5.1:-77348861 | None:intergenic |
| GCTGTTGGTCAGATTAAAGC+AGG | 0.505927 | 5.1:+77355453 | MS.gene38022:CDS |
| ATCTGAGGCGCTGATTGGTA+TGG | 0.509293 | 5.1:+77347659 | MS.gene38022:CDS |
| AGCAGGCAAAGGAAGCGCAT+TGG | 0.509873 | 5.1:+77355361 | MS.gene38022:CDS |
| GAAATTTGATACAGTTACTT+CGG | 0.514281 | 5.1:+77356146 | MS.gene38022:intron |
| AACTGTAATGTCGGTGAAGA+TGG | 0.515053 | 5.1:+77351483 | MS.gene38022:CDS |
| AAGATCGCTCTTGACGTCGC+TGG | 0.516723 | 5.1:+77352553 | MS.gene38022:CDS |
| ATTTGATGAAGCACTGCGAA+TGG | 0.517360 | 5.1:+77350074 | MS.gene38022:CDS |
| GCTTTGCTCCTAATGTCTCC+AGG | 0.523451 | 5.1:+77351508 | MS.gene38022:CDS |
| GTTCCATCTGGAAACTCCAC+TGG | 0.529470 | 5.1:+77346456 | MS.gene38022:CDS |
| TTTGATGAAGCACTGCGAAT+GGG | 0.531009 | 5.1:+77350075 | MS.gene38022:CDS |
| TTTAAATCTCCTCAAACGTC+GGG | 0.533243 | 5.1:+77353893 | MS.gene38022:CDS |
| TACAGATCAACTTCAACAGT+TGG | 0.534257 | 5.1:-77346405 | None:intergenic |
| TTTATGCTGGTGAAGATTGG+AGG | 0.538769 | 5.1:+77356197 | MS.gene38022:CDS |
| AGGCAAAGGAAGCGCATTGG+GGG | 0.540352 | 5.1:+77355364 | MS.gene38022:CDS |
| TTATCCGTTGGCCTTGCTGT+TGG | 0.541949 | 5.1:+77355438 | MS.gene38022:CDS |
| GGAGATCAAGCAGTTTATGC+TGG | 0.544508 | 5.1:+77356184 | MS.gene38022:CDS |
| GGTCATTGAAGTGGTCAAGC+AGG | 0.547758 | 5.1:+77355344 | MS.gene38022:CDS |
| TCACCGACATTACAGTTATG+TGG | 0.549326 | 5.1:-77351477 | None:intergenic |
| TATCATAGCATGATCAATCT+GGG | 0.550616 | 5.1:-77347697 | None:intergenic |
| CAGGCAAAGGAAGCGCATTG+GGG | 0.551427 | 5.1:+77355363 | MS.gene38022:CDS |
| CTTTAAATCTCCTCAAACGT+CGG | 0.555208 | 5.1:+77353892 | MS.gene38022:CDS |
| GTGATCCAGGTCAACCAAGT+TGG | 0.555500 | 5.1:+77355309 | MS.gene38022:intron |
| TCTAATCGCTCTCCTCTGCA+AGG | 0.555562 | 5.1:-77355480 | None:intergenic |
| GGCCTCCTTTACAAGATCCA+GGG | 0.557045 | 5.1:-77352500 | None:intergenic |
| AAAGGTGTATATCTAGGAAA+CGG | 0.559992 | 5.1:+77347600 | MS.gene38022:CDS |
| TCAATCTGGGACTGCAGTGT+TGG | 0.560963 | 5.1:-77347684 | None:intergenic |
| AGAAGCCCTGGATCTTGTAA+AGG | 0.562004 | 5.1:+77352495 | MS.gene38022:CDS |
| ATAATACCTGGCAAATTCCA+AGG | 0.562772 | 5.1:-77354141 | None:intergenic |
| CCATTGCTGCATGTAAAGCT+GGG | 0.568225 | 5.1:+77348663 | MS.gene38022:CDS |
| AGGCTGTTGAGTTACGGGAT+GGG | 0.576344 | 5.1:+77347574 | MS.gene38022:CDS |
| AGATATTACGATGAAGAGAA+CGG | 0.576440 | 5.1:-77346221 | None:intergenic |
| TGAAGTGGTCAAGCAGGCAA+AGG | 0.584411 | 5.1:+77355350 | MS.gene38022:CDS |
| GATGAAGAGAACGGGGTCGG+AGG | 0.585100 | 5.1:-77346212 | None:intergenic |
| ATAAGCGGTGGAAAACATGC+TGG | 0.585973 | 5.1:+77348882 | MS.gene38022:CDS |
| ATGCCAGTGGAGTTTCCAGA+TGG | 0.588263 | 5.1:-77346459 | None:intergenic |
| TTTATCAAGATATTCTTGAA+CGG | 0.588908 | 5.1:-77346134 | None:intergenic |
| GAGGCTGTTGAGTTACGGGA+TGG | 0.592923 | 5.1:+77347573 | MS.gene38022:CDS |
| AATGATTCTGCAGGTAGTAG+GGG | 0.598973 | 5.1:+77354748 | MS.gene38022:intron |
| TACGATGAAGAGAACGGGGT+CGG | 0.599870 | 5.1:-77346215 | None:intergenic |
| TCAGCGATGTGCTTGTAGAG+TGG | 0.606837 | 5.1:-77348810 | None:intergenic |
| GTGGTGACATCTCACAGAAG+TGG | 0.607193 | 5.1:+77355387 | MS.gene38022:CDS |
| CCCAGCTTTACATGCAGCAA+TGG | 0.608735 | 5.1:-77348663 | None:intergenic |
| AAGCAGGAACAGGAAGCACT+GGG | 0.619719 | 5.1:-77348851 | None:intergenic |
| TTGTACAAGGAACTATGTAC+CGG | 0.620708 | 5.1:+77353950 | MS.gene38022:CDS |
| AGCCCTGGATCTTGTAAAGG+AGG | 0.621208 | 5.1:+77352498 | MS.gene38022:CDS |
| GAGTTACGGGATGGGGACAA+AGG | 0.621649 | 5.1:+77347582 | MS.gene38022:CDS |
| CCGCTTATAACAGTAAAAGC+AGG | 0.623476 | 5.1:-77348867 | None:intergenic |
| AGGCAAATTCTTGATAGTAG+AGG | 0.625871 | 5.1:+77346378 | MS.gene38022:CDS |
| CCAAGTTGGAACTGTTACCG+AGG | 0.633838 | 5.1:+77355323 | MS.gene38022:CDS |
| AAAGCAGGCGCTCCTTGCAG+AGG | 0.636542 | 5.1:+77355468 | MS.gene38022:CDS |
| ATTAACATCTTACATGCCAG+TGG | 0.640506 | 5.1:-77346472 | None:intergenic |
| CTTGACCACTTCAATGACCT+CGG | 0.640611 | 5.1:-77355340 | None:intergenic |
| TATAAATGAGAAAATATCTG+AGG | 0.641810 | 5.1:+77347644 | MS.gene38022:CDS |
| CTTTAATCTGACCAACAGCA+AGG | 0.642755 | 5.1:-77355449 | None:intergenic |
| TGTTACCGAGGTCATTGAAG+TGG | 0.644212 | 5.1:+77355335 | MS.gene38022:CDS |
| GGCAAAGGAAGCGCATTGGG+GGG | 0.654274 | 5.1:+77355365 | MS.gene38022:CDS |
| GGATATGATAGAATTGTACA+AGG | 0.666629 | 5.1:+77353937 | MS.gene38022:CDS |
| TGATTACGAAAGTAAAAGCG+AGG | 0.674184 | 5.1:+77346358 | MS.gene38022:CDS |
| CTGACCAACAGCAAGGCCAA+CGG | 0.676271 | 5.1:-77355442 | None:intergenic |
| AATAGAAGATCCATTTGACA+AGG | 0.686135 | 5.1:+77354087 | MS.gene38022:CDS |
| GGGCCACATAACTGTAATGT+CGG | 0.693032 | 5.1:+77351474 | MS.gene38022:CDS |
| CATTGCTGCATGTAAAGCTG+GGG | 0.699717 | 5.1:+77348664 | MS.gene38022:CDS |
| TGTAATGTCGGTGAAGATGG+TGG | 0.702441 | 5.1:+77351486 | MS.gene38022:CDS |
| ATATTACGATGAAGAGAACG+GGG | 0.743082 | 5.1:-77346219 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 77346130 | 77356225 | 77346130 | ID=MS.gene38022 |
| chr5.1 | mRNA | 77346130 | 77356225 | 77346130 | ID=MS.gene38022.t1;Parent=MS.gene38022 |
| chr5.1 | exon | 77346130 | 77346234 | 77346130 | ID=MS.gene38022.t1.exon1;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77346130 | 77346234 | 77346130 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77346327 | 77346481 | 77346327 | ID=MS.gene38022.t1.exon2;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77346327 | 77346481 | 77346327 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77347569 | 77347743 | 77347569 | ID=MS.gene38022.t1.exon3;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77347569 | 77347743 | 77347569 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77348629 | 77348700 | 77348629 | ID=MS.gene38022.t1.exon4;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77348629 | 77348700 | 77348629 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77348807 | 77348923 | 77348807 | ID=MS.gene38022.t1.exon5;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77348807 | 77348923 | 77348807 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77350042 | 77350122 | 77350042 | ID=MS.gene38022.t1.exon6;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77350042 | 77350122 | 77350042 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77351453 | 77351529 | 77351453 | ID=MS.gene38022.t1.exon7;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77351453 | 77351529 | 77351453 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77352489 | 77352592 | 77352489 | ID=MS.gene38022.t1.exon8;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77352489 | 77352592 | 77352489 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77353873 | 77353971 | 77353873 | ID=MS.gene38022.t1.exon9;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77353873 | 77353971 | 77353873 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77354071 | 77354156 | 77354071 | ID=MS.gene38022.t1.exon10;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77354071 | 77354156 | 77354071 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77354761 | 77354847 | 77354761 | ID=MS.gene38022.t1.exon11;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77354761 | 77354847 | 77354761 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77355318 | 77355515 | 77355318 | ID=MS.gene38022.t1.exon12;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77355318 | 77355515 | 77355318 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| chr5.1 | exon | 77356160 | 77356225 | 77356160 | ID=MS.gene38022.t1.exon13;Parent=MS.gene38022.t1 |
| chr5.1 | CDS | 77356160 | 77356225 | 77356160 | ID=cds.MS.gene38022.t1;Parent=MS.gene38022.t1 |
| Gene Sequence |
| Protein sequence |
