Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38168.t1 | XP_003618009.2 | 97 | 164 | 4 | 1 | 1 | 163 | 1 | 164 | 2.50E-82 | 314.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38168.t1 | G7K4Z5 | 97.0 | 164 | 4 | 1 | 1 | 163 | 1 | 164 | 1.8e-82 | 314.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056314 | MS.gene38168 | 0.801885 | 7.29E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38168.t1 | MTR_5g097970 | 96.951 | 164 | 4 | 1 | 1 | 163 | 1 | 164 | 3.93e-115 | 323 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38168.t1 | AT2G04790 | 61.379 | 145 | 53 | 2 | 15 | 156 | 34 | 178 | 1.90e-64 | 195 |
| MS.gene38168.t1 | AT2G04790 | 61.379 | 145 | 53 | 2 | 15 | 156 | 36 | 180 | 1.98e-64 | 196 |
| MS.gene38168.t1 | AT2G04790 | 61.379 | 145 | 53 | 2 | 15 | 156 | 17 | 161 | 3.35e-64 | 194 |
| MS.gene38168.t1 | AT2G04790 | 64.394 | 132 | 44 | 2 | 15 | 143 | 36 | 167 | 3.61e-61 | 187 |
| MS.gene38168.t1 | AT2G04790 | 64.394 | 132 | 44 | 2 | 15 | 143 | 34 | 165 | 4.92e-61 | 186 |
| MS.gene38168.t1 | AT2G04790 | 64.394 | 132 | 44 | 2 | 15 | 143 | 17 | 148 | 6.54e-61 | 186 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 5.16e-32 | 111 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 5.16e-32 | 111 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 17 | 93 | 9.64e-32 | 110 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 17 | 93 | 9.64e-32 | 110 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 1.27e-31 | 110 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 36 | 112 | 1.27e-31 | 110 |
| MS.gene38168.t1 | AT2G04790 | 64.935 | 77 | 25 | 1 | 15 | 89 | 17 | 93 | 2.06e-31 | 109 |
| MS.gene38168.t1 | AT2G04790 | 72.727 | 44 | 12 | 0 | 15 | 58 | 36 | 79 | 2.98e-18 | 75.1 |
| MS.gene38168.t1 | AT2G04790 | 72.727 | 44 | 12 | 0 | 15 | 58 | 36 | 79 | 2.98e-18 | 75.1 |
| MS.gene38168.t1 | AT2G04790 | 72.727 | 44 | 12 | 0 | 15 | 58 | 36 | 79 | 2.98e-18 | 75.1 |
Find 37 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCTTACTCCCTTAATATT+TGG | 0.106470 | 5.3:-79262509 | MS.gene38168:CDS |
| GCTGCAAATCCCAAATATTA+AGG | 0.224867 | 5.3:+79262499 | None:intergenic |
| TGCTTACTCCCTTAATATTT+GGG | 0.251158 | 5.3:-79262508 | MS.gene38168:CDS |
| TATATATCTTCAGGATCTTT+TGG | 0.303960 | 5.3:+79262272 | None:intergenic |
| ATGTGGCAGCAATGCCTCTA+AGG | 0.323320 | 5.3:-79262566 | MS.gene38168:CDS |
| TTGGGATTTGCAGCATTTCA+TGG | 0.365079 | 5.3:-79262490 | MS.gene38168:CDS |
| GGTCCCTTTGAAGCCCTTAG+AGG | 0.394810 | 5.3:+79262552 | None:intergenic |
| GTCAATTTAAAGGTTTGGTC+AGG | 0.403272 | 5.3:-79261763 | MS.gene38168:intron |
| CAAACCTTCCTCACCTTCAA+AGG | 0.430343 | 5.3:-79262460 | MS.gene38168:intron |
| CTGCAAATCCCAAATATTAA+GGG | 0.434798 | 5.3:+79262500 | None:intergenic |
| CGAAGCAAATGTTGGCTAGT+TGG | 0.443608 | 5.3:-79262066 | MS.gene38168:CDS |
| AATAGGATTGAAACCTTTGA+AGG | 0.455552 | 5.3:+79262447 | None:intergenic |
| AATGCCTCTAAGGGCTTCAA+AGG | 0.472734 | 5.3:-79262556 | MS.gene38168:CDS |
| AGTGCCGCATATATATCTTC+AGG | 0.473960 | 5.3:+79262263 | None:intergenic |
| TGGGAAACAGATTTGAGAAT+CGG | 0.477510 | 5.3:-79261994 | MS.gene38168:CDS |
| ACTAGCCAACATTTGCTTCG+CGG | 0.493748 | 5.3:+79262069 | None:intergenic |
| GGTTTGGTCAGGCAACTCAT+TGG | 0.502217 | 5.3:-79261752 | MS.gene38168:CDS |
| GAAACCTTTGAAGGTGAGGA+AGG | 0.532186 | 5.3:+79262456 | None:intergenic |
| AGGCAGAGAGGAAGTGTATG+TGG | 0.544412 | 5.3:-79262583 | MS.gene38168:CDS |
| TTATCTGGTAGAGCTGTGCC+AGG | 0.545939 | 5.3:-79262233 | MS.gene38168:intron |
| TCAAAGACAAGAATGGTAAG+AGG | 0.550312 | 5.3:-79262603 | MS.gene38168:CDS |
| AGTTGTATCAAAGACAAGAA+TGG | 0.561294 | 5.3:-79262610 | MS.gene38168:CDS |
| TGTGGCAGCAATGCCTCTAA+GGG | 0.570128 | 5.3:-79262565 | MS.gene38168:CDS |
| GATTGTCGCGATTATACCAA+TGG | 0.578050 | 5.3:-79261964 | MS.gene38168:intron |
| ATGCCTCTAAGGGCTTCAAA+GGG | 0.584284 | 5.3:-79262555 | MS.gene38168:CDS |
| GATTGAAACCTTTGAAGGTG+AGG | 0.593749 | 5.3:+79262452 | None:intergenic |
| GATTGCCGCGAAGCAAATGT+TGG | 0.594551 | 5.3:-79262074 | MS.gene38168:CDS |
| GCTGAAACGTTTGAGAAACC+AGG | 0.597971 | 5.3:-79261717 | MS.gene38168:CDS |
| GCAGCAGACATAAGCAATTG+TGG | 0.599579 | 5.3:+79262528 | None:intergenic |
| TCACAGGAGCTGTTCTTGTG+AGG | 0.601866 | 5.3:-79262107 | MS.gene38168:intron |
| TCATCTACCTCTCGCTACCC+TGG | 0.604397 | 5.3:+79261699 | None:intergenic |
| AAGAATGGTAAGAGGCAGAG+AGG | 0.650232 | 5.3:-79262595 | MS.gene38168:CDS |
| CTGAAACGTTTGAGAAACCA+GGG | 0.653890 | 5.3:-79261716 | MS.gene38168:CDS |
| TGAGAAACCAGGGTAGCGAG+AGG | 0.656057 | 5.3:-79261706 | MS.gene38168:CDS |
| ATATGCAGAAGCAGATGCTG+TGG | 0.680638 | 5.3:-79262043 | MS.gene38168:CDS |
| AGATCCTGAAGATATATATG+CGG | 0.681441 | 5.3:-79262267 | MS.gene38168:CDS |
| GCAGACATAAGCAATTGTGG+TGG | 0.708742 | 5.3:+79262531 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTCGTTAAAAAAAAGTTT+AGG | - | chr5.3:79261920-79261939 | MS.gene38168:intron | 15.0% |
| !! | AACAACATATTAATTACCAT+TGG | + | chr5.3:79262376-79262395 | None:intergenic | 20.0% |
| !! | AAGTGAATTCAACAAAAAAT+GGG | - | chr5.3:79262308-79262327 | MS.gene38168:intron | 20.0% |
| !! | TGATAATATACAAACACTTA+TGG | + | chr5.3:79262455-79262474 | None:intergenic | 20.0% |
| !!! | TATTGCTTGTATTTATTTTC+AGG | - | chr5.3:79262006-79262025 | MS.gene38168:CDS | 20.0% |
| !!! | TGAATTTTTGGTCAATTTAA+AGG | - | chr5.3:79262548-79262567 | MS.gene38168:CDS | 20.0% |
| !!! | TTCAATCTGAATTGAATTTT+TGG | - | chr5.3:79262536-79262555 | MS.gene38168:CDS | 20.0% |
| ! | ATTCTGTTCATATAATTCAC+AGG | - | chr5.3:79262198-79262217 | MS.gene38168:intron | 25.0% |
| ! | CAAGTGAATTCAACAAAAAA+TGG | - | chr5.3:79262307-79262326 | MS.gene38168:intron | 25.0% |
| ! | GTGAATTATATGAACAGAAT+TGG | + | chr5.3:79262198-79262217 | None:intergenic | 25.0% |
| !! | TATATATCTTCAGGATCTTT+TGG | + | chr5.3:79262052-79262071 | None:intergenic | 25.0% |
| !! | TTTTGGTCAATTTAAAGGTT+TGG | - | chr5.3:79262553-79262572 | MS.gene38168:CDS | 25.0% |
| AATAGGATTGAAACCTTTGA+AGG | + | chr5.3:79261877-79261896 | None:intergenic | 30.0% | |
| ACATAACTCTATACACAGAT+AGG | + | chr5.3:79262427-79262446 | None:intergenic | 30.0% | |
| AGATCCTGAAGATATATATG+CGG | - | chr5.3:79262054-79262073 | MS.gene38168:CDS | 30.0% | |
| AGTTGTATCAAAGACAAGAA+TGG | - | chr5.3:79261711-79261730 | MS.gene38168:CDS | 30.0% | |
| CTGCAAATCCCAAATATTAA+GGG | + | chr5.3:79261824-79261843 | None:intergenic | 30.0% | |
| TAACGAAAAACCGATAATGT+AGG | + | chr5.3:79261909-79261928 | None:intergenic | 30.0% | |
| TGCTTACTCCCTTAATATTT+GGG | - | chr5.3:79261813-79261832 | MS.gene38168:intron | 30.0% | |
| ! | TAACTTGTGAACTTTCTGAT+TGG | - | chr5.3:79262131-79262150 | MS.gene38168:intron | 30.0% |
| AAAAACCGATAATGTAGGAG+AGG | + | chr5.3:79261904-79261923 | None:intergenic | 35.0% | |
| AATAAAGTCCAAATCTCACC+TGG | + | chr5.3:79262109-79262128 | None:intergenic | 35.0% | |
| AATGTAGGAGAGGAATGAAT+AGG | + | chr5.3:79261894-79261913 | None:intergenic | 35.0% | |
| CTGCTTACTCCCTTAATATT+TGG | - | chr5.3:79261812-79261831 | MS.gene38168:intron | 35.0% | |
| GCTGCAAATCCCAAATATTA+AGG | + | chr5.3:79261825-79261844 | None:intergenic | 35.0% | |
| TCAAAGACAAGAATGGTAAG+AGG | - | chr5.3:79261718-79261737 | MS.gene38168:CDS | 35.0% | |
| TCATTCCTCTCCTACATTAT+CGG | - | chr5.3:79261896-79261915 | MS.gene38168:intron | 35.0% | |
| TGGGAAACAGATTTGAGAAT+CGG | - | chr5.3:79262327-79262346 | MS.gene38168:intron | 35.0% | |
| ! | GTCAATTTAAAGGTTTGGTC+AGG | - | chr5.3:79262558-79262577 | MS.gene38168:CDS | 35.0% |
| AGTGCCGCATATATATCTTC+AGG | + | chr5.3:79262061-79262080 | None:intergenic | 40.0% | |
| CTGAAACGTTTGAGAAACCA+GGG | - | chr5.3:79262605-79262624 | MS.gene38168:CDS | 40.0% | |
| GATTGTCGCGATTATACCAA+TGG | - | chr5.3:79262357-79262376 | MS.gene38168:intron | 40.0% | |
| TTGGGATTTGCAGCATTTCA+TGG | - | chr5.3:79261831-79261850 | MS.gene38168:intron | 40.0% | |
| ! | GATTGAAACCTTTGAAGGTG+AGG | + | chr5.3:79261872-79261891 | None:intergenic | 40.0% |
| AAGAATGGTAAGAGGCAGAG+AGG | - | chr5.3:79261726-79261745 | MS.gene38168:CDS | 45.0% | |
| ACTAGCCAACATTTGCTTCG+CGG | + | chr5.3:79262255-79262274 | None:intergenic | 45.0% | |
| ATATGCAGAAGCAGATGCTG+TGG | - | chr5.3:79262278-79262297 | MS.gene38168:CDS | 45.0% | |
| CAAACCTTCCTCACCTTCAA+AGG | - | chr5.3:79261861-79261880 | MS.gene38168:intron | 45.0% | |
| GCAGACATAAGCAATTGTGG+TGG | + | chr5.3:79261793-79261812 | None:intergenic | 45.0% | |
| GCAGCAGACATAAGCAATTG+TGG | + | chr5.3:79261796-79261815 | None:intergenic | 45.0% | |
| GCTGAAACGTTTGAGAAACC+AGG | - | chr5.3:79262604-79262623 | MS.gene38168:CDS | 45.0% | |
| TTAGACGCTCTCTAAGCTTG+AGG | + | chr5.3:79261978-79261997 | None:intergenic | 45.0% | |
| ! | AATGCCTCTAAGGGCTTCAA+AGG | - | chr5.3:79261765-79261784 | MS.gene38168:intron | 45.0% |
| ! | ATGCCTCTAAGGGCTTCAAA+GGG | - | chr5.3:79261766-79261785 | MS.gene38168:intron | 45.0% |
| ! | CGAAGCAAATGTTGGCTAGT+TGG | - | chr5.3:79262255-79262274 | MS.gene38168:CDS | 45.0% |
| ! | GAAACCTTTGAAGGTGAGGA+AGG | + | chr5.3:79261868-79261887 | None:intergenic | 45.0% |
| ATGTGGCAGCAATGCCTCTA+AGG | - | chr5.3:79261755-79261774 | MS.gene38168:intron | 50.0% | |
| GATTGCCGCGAAGCAAATGT+TGG | - | chr5.3:79262247-79262266 | MS.gene38168:CDS | 50.0% | |
| GTCCGAATGTTCACCATCGA+CGG | - | chr5.3:79261951-79261970 | MS.gene38168:intron | 50.0% | |
| GTCCGTCGATGGTGAACATT+CGG | + | chr5.3:79261956-79261975 | None:intergenic | 50.0% | |
| TCACAGGAGCTGTTCTTGTG+AGG | - | chr5.3:79262214-79262233 | MS.gene38168:intron | 50.0% | |
| TGTGGCAGCAATGCCTCTAA+GGG | - | chr5.3:79261756-79261775 | MS.gene38168:intron | 50.0% | |
| TTATCTGGTAGAGCTGTGCC+AGG | - | chr5.3:79262088-79262107 | MS.gene38168:CDS | 50.0% | |
| ! | AGGCAGAGAGGAAGTGTATG+TGG | - | chr5.3:79261738-79261757 | MS.gene38168:CDS | 50.0% |
| ! | GGTTTGGTCAGGCAACTCAT+TGG | - | chr5.3:79262569-79262588 | MS.gene38168:CDS | 50.0% |
| !! | GCGGCACTAGCAGTTTTATC+TGG | - | chr5.3:79262073-79262092 | MS.gene38168:CDS | 50.0% |
| CTAAGCTTGAGGTCCGTCGA+TGG | + | chr5.3:79261967-79261986 | None:intergenic | 55.0% | |
| GAGCTGTGCCAGGTGAGATT+TGG | - | chr5.3:79262098-79262117 | MS.gene38168:CDS | 55.0% | |
| TGAGAAACCAGGGTAGCGAG+AGG | - | chr5.3:79262615-79262634 | MS.gene38168:CDS | 55.0% | |
| !! | GGTCCCTTTGAAGCCCTTAG+AGG | + | chr5.3:79261772-79261791 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 79261703 | 79262640 | 79261703 | ID=MS.gene38168 |
| chr5.3 | mRNA | 79261703 | 79262640 | 79261703 | ID=MS.gene38168.t1;Parent=MS.gene38168 |
| chr5.3 | exon | 79262461 | 79262640 | 79262461 | ID=MS.gene38168.t1.exon1;Parent=MS.gene38168.t1 |
| chr5.3 | CDS | 79262461 | 79262640 | 79262461 | ID=cds.MS.gene38168.t1;Parent=MS.gene38168.t1 |
| chr5.3 | exon | 79262234 | 79262315 | 79262234 | ID=MS.gene38168.t1.exon2;Parent=MS.gene38168.t1 |
| chr5.3 | CDS | 79262234 | 79262315 | 79262234 | ID=cds.MS.gene38168.t1;Parent=MS.gene38168.t1 |
| chr5.3 | exon | 79261965 | 79262123 | 79261965 | ID=MS.gene38168.t1.exon3;Parent=MS.gene38168.t1 |
| chr5.3 | CDS | 79261965 | 79262123 | 79261965 | ID=cds.MS.gene38168.t1;Parent=MS.gene38168.t1 |
| chr5.3 | exon | 79261703 | 79261773 | 79261703 | ID=MS.gene38168.t1.exon4;Parent=MS.gene38168.t1 |
| chr5.3 | CDS | 79261703 | 79261773 | 79261703 | ID=cds.MS.gene38168.t1;Parent=MS.gene38168.t1 |
| Gene Sequence |
| Protein sequence |
