Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38234.t1 | XP_003617922.1 | 99.6 | 473 | 2 | 0 | 1 | 473 | 1 | 473 | 5.70E-268 | 932.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38234.t1 | Q9ZW34 | 82.4 | 472 | 83 | 0 | 1 | 472 | 1 | 472 | 4.9e-228 | 791.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38234.t1 | G7K4W1 | 99.6 | 473 | 2 | 0 | 1 | 473 | 1 | 473 | 4.1e-268 | 932.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene38234 | 0.841097 | 5.84E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene007304 | MS.gene38234 | PPI |
| MS.gene045798 | MS.gene38234 | PPI |
| MS.gene83234 | MS.gene38234 | PPI |
| MS.gene020556 | MS.gene38234 | PPI |
| MS.gene38234 | MS.gene78187 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38234.t1 | MTR_5g096970 | 99.577 | 473 | 2 | 0 | 1 | 473 | 1 | 473 | 0.0 | 970 |
| MS.gene38234.t1 | MTR_7g103620 | 59.361 | 438 | 167 | 7 | 45 | 472 | 2 | 438 | 1.37e-180 | 513 |
| MS.gene38234.t1 | MTR_2g056190 | 58.605 | 430 | 175 | 3 | 44 | 472 | 62 | 489 | 1.73e-177 | 506 |
| MS.gene38234.t1 | MTR_6g060570 | 56.979 | 437 | 159 | 8 | 47 | 472 | 5 | 423 | 2.11e-165 | 474 |
| MS.gene38234.t1 | MTR_7g103620 | 58.713 | 373 | 145 | 5 | 109 | 472 | 16 | 388 | 7.10e-152 | 438 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38234.t1 | AT2G29560 | 82.415 | 472 | 83 | 0 | 1 | 472 | 1 | 472 | 0.0 | 820 |
| MS.gene38234.t1 | AT2G36530 | 58.998 | 439 | 169 | 7 | 45 | 473 | 2 | 439 | 3.33e-179 | 509 |
| MS.gene38234.t1 | AT1G74030 | 58.503 | 441 | 173 | 5 | 40 | 472 | 37 | 475 | 1.25e-177 | 506 |
| MS.gene38234.t1 | AT2G36530 | 58.134 | 418 | 164 | 7 | 66 | 473 | 12 | 428 | 4.59e-167 | 478 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACGGGGTCGGAGGTTT+TGG | 0.248202 | 5.3:-78365835 | None:intergenic |
| TGAGACCTATCATCATTTAA+AGG | 0.262267 | 5.3:+78370257 | MS.gene38234:CDS |
| CCTTGACAAAACAGAGAAAA+AGG | 0.305149 | 5.3:+78367891 | MS.gene38234:CDS |
| GGATCTTCTATTGATACAAT+TGG | 0.313466 | 5.3:-78373131 | None:intergenic |
| TTGTAATGCTCTTCTTCTTA+AGG | 0.320244 | 5.3:+78373881 | MS.gene38234:CDS |
| GGCTCTGACGGCGGCATTGA+CGG | 0.326530 | 5.3:-78365814 | None:intergenic |
| TGACGGCGTCTTCGAGTTTA+CGG | 0.335239 | 5.3:-78365797 | None:intergenic |
| ACAGGCAAAGGAAGCGCATT+GGG | 0.339363 | 5.3:+78374417 | MS.gene38234:CDS |
| TCAAATCTGCTTGCTCCAAT+TGG | 0.357446 | 5.3:-78370213 | None:intergenic |
| ATCCATTTGACAAGGAAGAT+TGG | 0.376004 | 5.3:+78373150 | MS.gene38234:CDS |
| TCCATTGCTGCATGTAAAGC+TGG | 0.377107 | 5.3:+78368824 | MS.gene38234:CDS |
| TGAATGATTCTGCAGGTAGT+AGG | 0.386036 | 5.3:+78373801 | MS.gene38234:intron |
| GTATGAGGCTGTTGAGTTAC+GGG | 0.389583 | 5.3:+78367738 | MS.gene38234:CDS |
| ATCATAACCGGTTCTACTAA+TGG | 0.389720 | 5.3:-78371576 | None:intergenic |
| CAGTTTATGCTGGTGAAGAT+TGG | 0.398772 | 5.3:+78375249 | MS.gene38234:CDS |
| TTTCGTGCTTCTGTTCCATC+TGG | 0.400465 | 5.3:+78366067 | MS.gene38234:CDS |
| CTATCATAGCATGATCAATC+TGG | 0.408498 | 5.3:-78367867 | None:intergenic |
| TCCATTTGACAAGGAAGATT+GGG | 0.410260 | 5.3:+78373151 | MS.gene38234:CDS |
| AAATTCTGTCCCGACGTTTG+AGG | 0.413766 | 5.3:-78372957 | None:intergenic |
| GATGATTCATTTCAGGTATG+AGG | 0.417338 | 5.3:+78367723 | MS.gene38234:intron |
| AAGCACATCGCTGACCTGTC+TGG | 0.418545 | 5.3:+78368981 | MS.gene38234:CDS |
| TCCCAATCTTCCTTGTCAAA+TGG | 0.418960 | 5.3:-78373152 | None:intergenic |
| GGTATGAGGCTGTTGAGTTA+CGG | 0.423365 | 5.3:+78367737 | MS.gene38234:intron |
| TGGCCTCCTTTACAAGATCC+AGG | 0.431446 | 5.3:-78371556 | None:intergenic |
| TTCATAGCTGATTTATCCGT+TGG | 0.433997 | 5.3:+78374481 | MS.gene38234:CDS |
| AAAGCTTGCCTGGAGACATT+AGG | 0.436617 | 5.3:-78370560 | None:intergenic |
| AACAGGTAAAAGATATGATC+TGG | 0.438662 | 5.3:+78372923 | MS.gene38234:intron |
| GAAATCATGATTCTTCCAAT+TGG | 0.441103 | 5.3:+78370198 | MS.gene38234:CDS |
| AAAACATGCTGGAAATAATC+TGG | 0.443130 | 5.3:+78369055 | MS.gene38234:CDS |
| AATCACCTTTAAATGATGAT+AGG | 0.445742 | 5.3:-78370262 | None:intergenic |
| GATATTACGATGAAGAGAAC+GGG | 0.448791 | 5.3:-78365849 | None:intergenic |
| TAAAGCTGGGGCTGCTGAAA+AGG | 0.454878 | 5.3:+78368838 | MS.gene38234:CDS |
| GATCTTCTATTGATACAATT+GGG | 0.456790 | 5.3:-78373130 | None:intergenic |
| GGTCATTGAAGTGGTCAAAC+AGG | 0.458747 | 5.3:+78374399 | MS.gene38234:CDS |
| CTTCGGATTGAGGAAGAGCT+TGG | 0.459854 | 5.3:+78375218 | MS.gene38234:CDS |
| CTCAAGCCTTGGAATTTGCC+AGG | 0.463723 | 5.3:+78373190 | MS.gene38234:CDS |
| GAATGATTCTGCAGGTAGTA+GGG | 0.464232 | 5.3:+78373802 | MS.gene38234:intron |
| GGGGACAAAGGTGTATATCT+AGG | 0.471083 | 5.3:+78367763 | MS.gene38234:CDS |
| ATCAAGTATATCTCAAGCCT+TGG | 0.478687 | 5.3:+78373179 | MS.gene38234:CDS |
| CACCAGCTTTAGAGAAGCCC+TGG | 0.479064 | 5.3:+78371538 | MS.gene38234:intron |
| AAGGAGGCCATTAGTAGAAC+CGG | 0.479320 | 5.3:+78371569 | MS.gene38234:CDS |
| GACGGCGTCTTCGAGTTTAC+GGG | 0.479590 | 5.3:-78365796 | None:intergenic |
| AAAATATCTGAGGCGCTGAT+TGG | 0.482231 | 5.3:+78367823 | MS.gene38234:CDS |
| TGGAAATAATCTGGCAATTG+AGG | 0.486178 | 5.3:+78369064 | MS.gene38234:CDS |
| CCATTGCTGCATGTAAAGCT+GGG | 0.490656 | 5.3:+78368825 | MS.gene38234:CDS |
| AAAGCAGGAACAGGAAGCAC+TGG | 0.490991 | 5.3:-78369014 | None:intergenic |
| GATACAGTTACTTCGGATTG+AGG | 0.493277 | 5.3:+78375208 | MS.gene38234:intron |
| GGCTGTTGAGTTACGGGATG+GGG | 0.493888 | 5.3:+78367744 | MS.gene38234:CDS |
| ATAACAGTAAAAGCAGGAAC+AGG | 0.498354 | 5.3:-78369023 | None:intergenic |
| CCTCGGTCACAGTTCCAACT+TGG | 0.498921 | 5.3:-78374378 | None:intergenic |
| TACAAATCAACTTCAACAGT+TGG | 0.502701 | 5.3:-78366028 | None:intergenic |
| GCTGTTGGTCAGATTAAAGC+AGG | 0.505927 | 5.3:+78374508 | MS.gene38234:CDS |
| ATCTGAGGCGCTGATTGGTA+TGG | 0.509293 | 5.3:+78367828 | MS.gene38234:CDS |
| GAAATTTGATACAGTTACTT+CGG | 0.514281 | 5.3:+78375201 | MS.gene38234:intron |
| AACTGTAATGTCGGTGAAGA+TGG | 0.515053 | 5.3:+78370527 | MS.gene38234:CDS |
| AAGATCGCTCTTGACGTCGC+TGG | 0.516723 | 5.3:+78371608 | MS.gene38234:CDS |
| ATTTGATGAAGCACTGCGAA+TGG | 0.517360 | 5.3:+78370230 | MS.gene38234:CDS |
| GCTTTGCTCCTAATGTCTCC+AGG | 0.523451 | 5.3:+78370552 | MS.gene38234:CDS |
| GTTCCATCTGGAAACTCCAC+TGG | 0.529470 | 5.3:+78366079 | MS.gene38234:CDS |
| TTTGATGAAGCACTGCGAAT+GGG | 0.531009 | 5.3:+78370231 | MS.gene38234:CDS |
| TTTAAATCTCCTCAAACGTC+GGG | 0.533243 | 5.3:+78372948 | MS.gene38234:CDS |
| TTTATGCTGGTGAAGATTGG+AGG | 0.538769 | 5.3:+78375252 | MS.gene38234:CDS |
| TTATCCGTTGGCCTTGCTGT+TGG | 0.541949 | 5.3:+78374493 | MS.gene38234:CDS |
| AACAGGCAAAGGAAGCGCAT+TGG | 0.543307 | 5.3:+78374416 | MS.gene38234:CDS |
| GGAGATCAAGCAGTTTATGC+TGG | 0.544508 | 5.3:+78375239 | MS.gene38234:CDS |
| TCACCGACATTACAGTTATG+TGG | 0.549326 | 5.3:-78370521 | None:intergenic |
| TATCATAGCATGATCAATCT+GGG | 0.550616 | 5.3:-78367866 | None:intergenic |
| CTTTAAATCTCCTCAAACGT+CGG | 0.555208 | 5.3:+78372947 | MS.gene38234:CDS |
| GTGATCCAGGTCAACCAAGT+TGG | 0.555500 | 5.3:+78374364 | MS.gene38234:intron |
| TCTAATCGCTCTCCTCTGCA+AGG | 0.555562 | 5.3:-78374535 | None:intergenic |
| GGCCTCCTTTACAAGATCCA+GGG | 0.557045 | 5.3:-78371555 | None:intergenic |
| AAAGGTGTATATCTAGGAAA+CGG | 0.559992 | 5.3:+78367769 | MS.gene38234:CDS |
| TCAATCTGGGACTGCAGTGT+TGG | 0.560963 | 5.3:-78367853 | None:intergenic |
| AGAAGCCCTGGATCTTGTAA+AGG | 0.562004 | 5.3:+78371550 | MS.gene38234:CDS |
| ATAATACCTGGCAAATTCCA+AGG | 0.562772 | 5.3:-78373196 | None:intergenic |
| AGGCAAAGGAAGCGCATTGG+GGG | 0.571983 | 5.3:+78374419 | MS.gene38234:CDS |
| AGGCTGTTGAGTTACGGGAT+GGG | 0.576344 | 5.3:+78367743 | MS.gene38234:CDS |
| AGATATTACGATGAAGAGAA+CGG | 0.576440 | 5.3:-78365850 | None:intergenic |
| TGAAGTGGTCAAACAGGCAA+AGG | 0.579709 | 5.3:+78374405 | MS.gene38234:CDS |
| GATGAAGAGAACGGGGTCGG+AGG | 0.585100 | 5.3:-78365841 | None:intergenic |
| CCAAGTTGGAACTGTGACCG+AGG | 0.585510 | 5.3:+78374378 | MS.gene38234:CDS |
| ATAAGCGGTGGAAAACATGC+TGG | 0.585973 | 5.3:+78369044 | MS.gene38234:CDS |
| ATGCCAGTGGAGTTTCCAGA+TGG | 0.588263 | 5.3:-78366082 | None:intergenic |
| TTTATCAAGATATTCTTGAA+CGG | 0.588908 | 5.3:-78365763 | None:intergenic |
| GAGGCTGTTGAGTTACGGGA+TGG | 0.592923 | 5.3:+78367742 | MS.gene38234:CDS |
| CAGGCAAAGGAAGCGCATTG+GGG | 0.596386 | 5.3:+78374418 | MS.gene38234:CDS |
| AATGATTCTGCAGGTAGTAG+GGG | 0.598973 | 5.3:+78373803 | MS.gene38234:intron |
| TACGATGAAGAGAACGGGGT+CGG | 0.599870 | 5.3:-78365844 | None:intergenic |
| TTTGACCACTTCAATGACCT+CGG | 0.604404 | 5.3:-78374395 | None:intergenic |
| CCCAGCTTTACATGCAGCAA+TGG | 0.606579 | 5.3:-78368825 | None:intergenic |
| TCAGCGATGTGCTTGTAGAG+TGG | 0.606837 | 5.3:-78368972 | None:intergenic |
| GTGGTGACATCTCACAGAAG+TGG | 0.607193 | 5.3:+78374442 | MS.gene38234:CDS |
| AAGCAGGAACAGGAAGCACT+GGG | 0.619719 | 5.3:-78369013 | None:intergenic |
| TTGTACAAGGAACTATGTAC+CGG | 0.620708 | 5.3:+78373005 | MS.gene38234:CDS |
| AGCCCTGGATCTTGTAAAGG+AGG | 0.621208 | 5.3:+78371553 | MS.gene38234:CDS |
| GAGTTACGGGATGGGGACAA+AGG | 0.621649 | 5.3:+78367751 | MS.gene38234:CDS |
| CCGCTTATAACAGTAAAAGC+AGG | 0.623476 | 5.3:-78369029 | None:intergenic |
| AGGCAAATTCTTGATAGTAG+AGG | 0.625871 | 5.3:+78366001 | MS.gene38234:CDS |
| CATTGCTGCATGTAAAGCTG+GGG | 0.626891 | 5.3:+78368826 | MS.gene38234:CDS |
| AAAGCAGGCGCTCCTTGCAG+AGG | 0.636542 | 5.3:+78374523 | MS.gene38234:CDS |
| TGTGACCGAGGTCATTGAAG+TGG | 0.639474 | 5.3:+78374390 | MS.gene38234:CDS |
| ATTAACATCTTACATGCCAG+TGG | 0.640506 | 5.3:-78366095 | None:intergenic |
| TATAAATGAGAAAATATCTG+AGG | 0.641810 | 5.3:+78367813 | MS.gene38234:CDS |
| CTTTAATCTGACCAACAGCA+AGG | 0.642755 | 5.3:-78374504 | None:intergenic |
| GGCAAAGGAAGCGCATTGGG+GGG | 0.654274 | 5.3:+78374420 | MS.gene38234:CDS |
| GGATATGATAGAATTGTACA+AGG | 0.666629 | 5.3:+78372992 | MS.gene38234:CDS |
| TGATTACGAAAGTAAAAGCG+AGG | 0.674184 | 5.3:+78365981 | MS.gene38234:CDS |
| CTGACCAACAGCAAGGCCAA+CGG | 0.676271 | 5.3:-78374497 | None:intergenic |
| AATAGAAGATCCATTTGACA+AGG | 0.686135 | 5.3:+78373142 | MS.gene38234:CDS |
| GGGCCACATAACTGTAATGT+CGG | 0.693032 | 5.3:+78370518 | MS.gene38234:CDS |
| TGTAATGTCGGTGAAGATGG+TGG | 0.702441 | 5.3:+78370530 | MS.gene38234:CDS |
| ATATTACGATGAAGAGAACG+GGG | 0.743082 | 5.3:-78365848 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 78365759 | 78375280 | 78365759 | ID=MS.gene38234 |
| chr5.3 | mRNA | 78365759 | 78375280 | 78365759 | ID=MS.gene38234.t1;Parent=MS.gene38234 |
| chr5.3 | exon | 78365759 | 78365863 | 78365759 | ID=MS.gene38234.t1.exon1;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78365759 | 78365863 | 78365759 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78365950 | 78366104 | 78365950 | ID=MS.gene38234.t1.exon2;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78365950 | 78366104 | 78365950 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78367738 | 78367912 | 78367738 | ID=MS.gene38234.t1.exon3;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78367738 | 78367912 | 78367738 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78368791 | 78368862 | 78368791 | ID=MS.gene38234.t1.exon4;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78368791 | 78368862 | 78368791 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78368969 | 78369085 | 78368969 | ID=MS.gene38234.t1.exon5;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78368969 | 78369085 | 78368969 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78370198 | 78370278 | 78370198 | ID=MS.gene38234.t1.exon6;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78370198 | 78370278 | 78370198 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78370497 | 78370573 | 78370497 | ID=MS.gene38234.t1.exon7;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78370497 | 78370573 | 78370497 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78371544 | 78371647 | 78371544 | ID=MS.gene38234.t1.exon8;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78371544 | 78371647 | 78371544 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78372928 | 78373026 | 78372928 | ID=MS.gene38234.t1.exon9;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78372928 | 78373026 | 78372928 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78373126 | 78373211 | 78373126 | ID=MS.gene38234.t1.exon10;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78373126 | 78373211 | 78373126 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78373816 | 78373902 | 78373816 | ID=MS.gene38234.t1.exon11;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78373816 | 78373902 | 78373816 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78374373 | 78374570 | 78374373 | ID=MS.gene38234.t1.exon12;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78374373 | 78374570 | 78374373 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| chr5.3 | exon | 78375215 | 78375280 | 78375215 | ID=MS.gene38234.t1.exon13;Parent=MS.gene38234.t1 |
| chr5.3 | CDS | 78375215 | 78375280 | 78375215 | ID=cds.MS.gene38234.t1;Parent=MS.gene38234.t1 |
| Gene Sequence |
| Protein sequence |
