Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38395.t1 | XP_013460462.1 | 86.9 | 236 | 30 | 1 | 2 | 237 | 341 | 575 | 1.90E-115 | 425.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38395.t1 | Q4F883 | 40.8 | 233 | 120 | 7 | 1 | 221 | 300 | 526 | 2.7e-41 | 170.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38395.t1 | A0A072V8I6 | 86.9 | 236 | 30 | 1 | 2 | 237 | 341 | 575 | 1.4e-115 | 425.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053647 | MS.gene38395 | 0.814892 | 1.22E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38395.t1 | MTR_3g467360 | 86.864 | 236 | 30 | 1 | 2 | 237 | 341 | 575 | 1.50e-151 | 434 |
| MS.gene38395.t1 | MTR_3g467360 | 86.864 | 236 | 30 | 1 | 2 | 237 | 347 | 581 | 1.92e-151 | 434 |
| MS.gene38395.t1 | MTR_6g007975 | 75.105 | 237 | 58 | 1 | 1 | 237 | 342 | 577 | 1.03e-124 | 365 |
| MS.gene38395.t1 | MTR_6g007985 | 67.932 | 237 | 75 | 1 | 1 | 237 | 266 | 501 | 1.69e-114 | 337 |
| MS.gene38395.t1 | MTR_6g008015 | 67.932 | 237 | 75 | 1 | 1 | 237 | 343 | 578 | 8.40e-114 | 337 |
| MS.gene38395.t1 | MTR_6g008055 | 66.245 | 237 | 75 | 2 | 1 | 237 | 344 | 575 | 3.08e-105 | 315 |
| MS.gene38395.t1 | MTR_6g007980 | 60.417 | 240 | 88 | 4 | 1 | 236 | 340 | 576 | 1.25e-94 | 288 |
| MS.gene38395.t1 | MTR_6g007980 | 60.417 | 240 | 88 | 4 | 1 | 236 | 341 | 577 | 1.28e-94 | 288 |
| MS.gene38395.t1 | MTR_6g007980 | 60.417 | 240 | 88 | 4 | 1 | 236 | 339 | 575 | 1.30e-94 | 288 |
| MS.gene38395.t1 | MTR_0286s0040 | 66.845 | 187 | 37 | 2 | 51 | 237 | 226 | 387 | 3.30e-79 | 243 |
| MS.gene38395.t1 | MTR_3g467350 | 86.735 | 98 | 13 | 0 | 140 | 237 | 385 | 482 | 5.77e-54 | 180 |
| MS.gene38395.t1 | MTR_3g467350 | 68.182 | 66 | 21 | 0 | 1 | 66 | 323 | 388 | 8.22e-28 | 110 |
| MS.gene38395.t1 | MTR_3g078633 | 28.230 | 209 | 112 | 8 | 5 | 201 | 417 | 599 | 1.01e-13 | 70.5 |
| MS.gene38395.t1 | MTR_3g078633 | 28.230 | 209 | 112 | 8 | 5 | 201 | 345 | 527 | 1.27e-13 | 70.1 |
| MS.gene38395.t1 | MTR_8g494200 | 26.389 | 216 | 139 | 6 | 6 | 208 | 403 | 611 | 1.86e-13 | 69.7 |
| MS.gene38395.t1 | MTR_3g079340 | 26.244 | 221 | 134 | 7 | 5 | 216 | 413 | 613 | 5.16e-12 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38395.t1 | AT5G14930 | 40.506 | 237 | 115 | 7 | 1 | 221 | 300 | 526 | 4.70e-49 | 169 |
| MS.gene38395.t1 | AT3G48080 | 28.054 | 221 | 126 | 7 | 7 | 202 | 392 | 604 | 2.93e-11 | 63.2 |
Find 42 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCACCTTCACTATAGTATT+TGG | 0.184967 | 3.4:+64134347 | None:intergenic |
| TGACTATAGATTCTTGCTTT+TGG | 0.196604 | 3.4:-64134197 | MS.gene38395:CDS |
| GAATGAGTATAAAGAAATTA+AGG | 0.234395 | 3.4:-64134004 | MS.gene38395:CDS |
| GAAGTTGAGATATATGTTTA+TGG | 0.266083 | 3.4:-64134093 | MS.gene38395:CDS |
| ACAGTTGGAGGAGTGGTTAT+TGG | 0.276000 | 3.4:-64134286 | MS.gene38395:CDS |
| TTTCGTACCCGTTGGTTATA+TGG | 0.328618 | 3.4:-64134411 | MS.gene38395:CDS |
| AAAGCTGCACCTTCTCTTTG+TGG | 0.372497 | 3.4:+64134432 | None:intergenic |
| AGTGTCTCCTGAGATATTTC+TGG | 0.398278 | 3.4:-64134052 | MS.gene38395:CDS |
| CACTATTTACTGGGAAAAGA+TGG | 0.411799 | 3.4:-64134475 | MS.gene38395:CDS |
| CTCCAACTGTTTGAAATGTT+CGG | 0.413686 | 3.4:+64134299 | None:intergenic |
| AAGATTAAGCAGTATGCTAT+AGG | 0.429206 | 3.4:-64133934 | MS.gene38395:CDS |
| CCAACAGCATCATGGTCAAT+TGG | 0.440770 | 3.4:+64134513 | None:intergenic |
| AAGTTGAGATATATGTTTAT+GGG | 0.442283 | 3.4:-64134092 | MS.gene38395:CDS |
| GAATGACATGAAGGTACATA+TGG | 0.447625 | 3.4:-64134613 | None:intergenic |
| AATGAGTATAAAGAAATTAA+GGG | 0.468466 | 3.4:-64134003 | MS.gene38395:CDS |
| GTCCGAACATTTCAAACAGT+TGG | 0.479840 | 3.4:-64134301 | MS.gene38395:CDS |
| AAAACTAAGAATGAAAATGC+AGG | 0.513971 | 3.4:-64134567 | MS.gene38395:CDS |
| CGCAAAGCAGCTATATTCGT+TGG | 0.516721 | 3.4:-64134029 | MS.gene38395:CDS |
| AAGACTATGTTAATAACAAA+CGG | 0.517463 | 3.4:-64134323 | MS.gene38395:CDS |
| TTGAAGGCTGTCAAAGAGAA+AGG | 0.523548 | 3.4:-64134138 | MS.gene38395:CDS |
| CTTTGCGCCAGAAATATCTC+AGG | 0.527302 | 3.4:+64134045 | None:intergenic |
| CGTACCCGTTGGTTATATGG+AGG | 0.529527 | 3.4:-64134408 | MS.gene38395:CDS |
| CAGCATCATGGTCAATTGGT+TGG | 0.530941 | 3.4:+64134517 | None:intergenic |
| CAATTGACCATGATGCTGTT+GGG | 0.534264 | 3.4:-64134512 | MS.gene38395:CDS |
| TATGGAACCCAACAGCATCA+TGG | 0.536170 | 3.4:+64134505 | None:intergenic |
| TACATATGGCCCGACTTGAA+TGG | 0.546812 | 3.4:-64134599 | MS.gene38395:CDS |
| AGTCCCTCCATATAACCAAC+GGG | 0.547594 | 3.4:+64134404 | None:intergenic |
| CGAACATTTCAAACAGTTGG+AGG | 0.548469 | 3.4:-64134298 | MS.gene38395:CDS |
| GCCAAATACTATAGTGAAGG+TGG | 0.562312 | 3.4:-64134348 | MS.gene38395:CDS |
| ATTTCAAACAGTTGGAGGAG+TGG | 0.565495 | 3.4:-64134293 | MS.gene38395:CDS |
| CCAATTGACCATGATGCTGT+TGG | 0.574111 | 3.4:-64134513 | MS.gene38395:CDS |
| ATATGTTTATGGGTTGCTCA+AGG | 0.575375 | 3.4:-64134082 | MS.gene38395:CDS |
| AGGGACTACTTACAGAAGAA+TGG | 0.577212 | 3.4:-64134388 | MS.gene38395:CDS |
| GTACCCGTTGGTTATATGGA+GGG | 0.577415 | 3.4:-64134407 | MS.gene38395:CDS |
| TAGTATTTGGCAATAGCTAG+TGG | 0.602435 | 3.4:+64134360 | None:intergenic |
| TAGTACACATAGCAAGAATG+TGG | 0.602705 | 3.4:-64134229 | MS.gene38395:CDS |
| GAGATGAAGCCACAAAGAGA+AGG | 0.602952 | 3.4:-64134441 | MS.gene38395:CDS |
| AAACTAAGAATGAAAATGCA+GGG | 0.608258 | 3.4:-64134566 | MS.gene38395:CDS |
| TAGTCCCTCCATATAACCAA+CGG | 0.612991 | 3.4:+64134403 | None:intergenic |
| ATTGCCAAATACTATAGTGA+AGG | 0.613573 | 3.4:-64134351 | MS.gene38395:CDS |
| AAAGCAGCTATATTCGTTGG+TGG | 0.633457 | 3.4:-64134026 | MS.gene38395:CDS |
| GGCTGTCAAAGAGAAAGGCG+AGG | 0.695654 | 3.4:-64134133 | MS.gene38395:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAGTATAAAGAAATTAA+GGG | - | chr3.4:64134518-64134537 | MS.gene38395:CDS | 15.0% |
| !! | AAGACTATGTTAATAACAAA+CGG | - | chr3.4:64134198-64134217 | MS.gene38395:CDS | 20.0% |
| !! | AAGTTGAGATATATGTTTAT+GGG | - | chr3.4:64134429-64134448 | MS.gene38395:CDS | 20.0% |
| !! | ATAAAAAACTCACTATTTAC+TGG | - | chr3.4:64134036-64134055 | MS.gene38395:CDS | 20.0% |
| !! | GAATGAGTATAAAGAAATTA+AGG | - | chr3.4:64134517-64134536 | MS.gene38395:CDS | 20.0% |
| !! | TAAAAAACTCACTATTTACT+GGG | - | chr3.4:64134037-64134056 | MS.gene38395:CDS | 20.0% |
| !!! | AGTAAATAGTGAGTTTTTTA+TGG | + | chr3.4:64134037-64134056 | None:intergenic | 20.0% |
| ! | AAAACTAAGAATGAAAATGC+AGG | - | chr3.4:64133954-64133973 | MS.gene38395:CDS | 25.0% |
| ! | AAACTAAGAATGAAAATGCA+GGG | - | chr3.4:64133955-64133974 | MS.gene38395:CDS | 25.0% |
| ! | GAAGTTGAGATATATGTTTA+TGG | - | chr3.4:64134428-64134447 | MS.gene38395:CDS | 25.0% |
| AAGATTAAGCAGTATGCTAT+AGG | - | chr3.4:64134587-64134606 | MS.gene38395:CDS | 30.0% | |
| ATTGCCAAATACTATAGTGA+AGG | - | chr3.4:64134170-64134189 | MS.gene38395:CDS | 30.0% | |
| ! | TCTTTTTGTACCATTCAAGT+CGG | + | chr3.4:64133935-64133954 | None:intergenic | 30.0% |
| ! | TGACTATAGATTCTTGCTTT+TGG | - | chr3.4:64134324-64134343 | MS.gene38395:CDS | 30.0% |
| !! | GACTATAGATTCTTGCTTTT+GGG | - | chr3.4:64134325-64134344 | MS.gene38395:CDS | 30.0% |
| ACCACCTTCACTATAGTATT+TGG | + | chr3.4:64134177-64134196 | None:intergenic | 35.0% | |
| CACTATTTACTGGGAAAAGA+TGG | - | chr3.4:64134046-64134065 | MS.gene38395:CDS | 35.0% | |
| TAGTACACATAGCAAGAATG+TGG | - | chr3.4:64134292-64134311 | MS.gene38395:CDS | 35.0% | |
| TAGTATTTGGCAATAGCTAG+TGG | + | chr3.4:64134164-64134183 | None:intergenic | 35.0% | |
| ! | ATATGTTTATGGGTTGCTCA+AGG | - | chr3.4:64134439-64134458 | MS.gene38395:CDS | 35.0% |
| ! | CTCCAACTGTTTGAAATGTT+CGG | + | chr3.4:64134225-64134244 | None:intergenic | 35.0% |
| ! | CTTTTTGTACCATTCAAGTC+GGG | + | chr3.4:64133934-64133953 | None:intergenic | 35.0% |
| AAAGCAGCTATATTCGTTGG+TGG | - | chr3.4:64134495-64134514 | MS.gene38395:CDS | 40.0% | |
| AGGGACTACTTACAGAAGAA+TGG | - | chr3.4:64134133-64134152 | MS.gene38395:CDS | 40.0% | |
| AGTGTCTCCTGAGATATTTC+TGG | - | chr3.4:64134469-64134488 | MS.gene38395:CDS | 40.0% | |
| ATTTCAAACAGTTGGAGGAG+TGG | - | chr3.4:64134228-64134247 | MS.gene38395:CDS | 40.0% | |
| CAATTGACCATGATGCTGTT+GGG | - | chr3.4:64134009-64134028 | MS.gene38395:CDS | 40.0% | |
| CGAACATTTCAAACAGTTGG+AGG | - | chr3.4:64134223-64134242 | MS.gene38395:CDS | 40.0% | |
| GCCAAATACTATAGTGAAGG+TGG | - | chr3.4:64134173-64134192 | MS.gene38395:CDS | 40.0% | |
| GTCCGAACATTTCAAACAGT+TGG | - | chr3.4:64134220-64134239 | MS.gene38395:CDS | 40.0% | |
| TAGTCCCTCCATATAACCAA+CGG | + | chr3.4:64134121-64134140 | None:intergenic | 40.0% | |
| TTGAAGGCTGTCAAAGAGAA+AGG | - | chr3.4:64134383-64134402 | MS.gene38395:CDS | 40.0% | |
| ! | GAAGAGGCTCTTCTTTTATG+CGG | - | chr3.4:64134356-64134375 | MS.gene38395:CDS | 40.0% |
| ! | TCTTTTATGCGGAGAGTTGA+AGG | - | chr3.4:64134367-64134386 | MS.gene38395:CDS | 40.0% |
| ! | TTTCGTACCCGTTGGTTATA+TGG | - | chr3.4:64134110-64134129 | MS.gene38395:CDS | 40.0% |
| AAAGCTGCACCTTCTCTTTG+TGG | + | chr3.4:64134092-64134111 | None:intergenic | 45.0% | |
| ACAGTTGGAGGAGTGGTTAT+TGG | - | chr3.4:64134235-64134254 | MS.gene38395:CDS | 45.0% | |
| AGTCCCTCCATATAACCAAC+GGG | + | chr3.4:64134120-64134139 | None:intergenic | 45.0% | |
| CAGCATCATGGTCAATTGGT+TGG | + | chr3.4:64134007-64134026 | None:intergenic | 45.0% | |
| CCAACAGCATCATGGTCAAT+TGG | + | chr3.4:64134011-64134030 | None:intergenic | 45.0% | |
| CCAATTGACCATGATGCTGT+TGG | - | chr3.4:64134008-64134027 | MS.gene38395:CDS | 45.0% | |
| CGCAAAGCAGCTATATTCGT+TGG | - | chr3.4:64134492-64134511 | MS.gene38395:CDS | 45.0% | |
| CTTTGCGCCAGAAATATCTC+AGG | + | chr3.4:64134479-64134498 | None:intergenic | 45.0% | |
| GAGATGAAGCCACAAAGAGA+AGG | - | chr3.4:64134080-64134099 | MS.gene38395:CDS | 45.0% | |
| TATGGAACCCAACAGCATCA+TGG | + | chr3.4:64134019-64134038 | None:intergenic | 45.0% | |
| ! | GTACCCGTTGGTTATATGGA+GGG | - | chr3.4:64134114-64134133 | MS.gene38395:CDS | 45.0% |
| ! | TACATATGGCCCGACTTGAA+TGG | - | chr3.4:64133922-64133941 | MS.gene38395:CDS | 45.0% |
| ! | CGTACCCGTTGGTTATATGG+AGG | - | chr3.4:64134113-64134132 | MS.gene38395:CDS | 50.0% |
| !! | TTCTTGCTTTTGGGCGCATG+TGG | - | chr3.4:64134334-64134353 | MS.gene38395:CDS | 50.0% |
| GGCTGTCAAAGAGAAAGGCG+AGG | - | chr3.4:64134388-64134407 | MS.gene38395:CDS | 55.0% | |
| ! | CTTTTGGGCGCATGTGGAAG+AGG | - | chr3.4:64134340-64134359 | MS.gene38395:CDS | 55.0% |
| ! | GTGCAGCTTTTCGTACCCGT+TGG | - | chr3.4:64134102-64134121 | MS.gene38395:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 64133915 | 64134628 | 64133915 | ID=MS.gene38395 |
| chr3.4 | mRNA | 64133915 | 64134628 | 64133915 | ID=MS.gene38395.t1;Parent=MS.gene38395 |
| chr3.4 | exon | 64133915 | 64134628 | 64133915 | ID=MS.gene38395.t1.exon1;Parent=MS.gene38395.t1 |
| chr3.4 | CDS | 64133915 | 64134628 | 64133915 | ID=cds.MS.gene38395.t1;Parent=MS.gene38395.t1 |
| Gene Sequence |
| Protein sequence |
