AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38512

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38512.t1 MTR_3g069780 66.667 366 73 2 249 568 9 371 1.22e-169 486
MS.gene38512.t1 MTR_3g438030 66.120 366 75 2 249 568 9 371 9.56e-168 481
MS.gene38512.t1 MTR_3g069520 66.485 367 70 5 249 568 9 369 3.99e-166 477
MS.gene38512.t1 MTR_3g448360 66.125 369 72 5 248 568 8 371 1.80e-165 475
MS.gene38512.t1 MTR_3g463960 56.216 370 110 4 247 567 21 387 7.72e-134 395
MS.gene38512.t1 MTR_3g463950 56.216 370 110 4 247 567 21 387 7.72e-134 395
MS.gene38512.t1 MTR_3g463970 56.216 370 110 4 247 567 21 387 7.72e-134 395
MS.gene38512.t1 MTR_3g463940 56.216 370 110 4 247 567 21 387 7.72e-134 395
MS.gene38512.t1 MTR_2g079090 55.464 366 100 6 249 567 9 358 6.60e-129 382
MS.gene38512.t1 MTR_0029s0050 55.738 366 99 6 249 567 9 358 1.17e-128 381
MS.gene38512.t1 MTR_3g463930 54.233 378 112 6 247 567 21 394 1.99e-126 377
MS.gene38512.t1 MTR_0029s0100 55.191 366 101 6 249 567 9 358 2.00e-126 375
MS.gene38512.t1 MTR_2g079080 54.645 366 103 6 249 567 9 358 3.59e-126 374
MS.gene38512.t1 MTR_2g043980 48.919 370 116 8 249 568 15 361 1.50e-105 322
MS.gene38512.t1 MTR_2g015650 42.627 373 157 8 247 568 8 374 8.37e-92 286
MS.gene38512.t1 MTR_2g015670 42.241 348 158 8 257 567 18 359 3.37e-85 269
MS.gene38512.t1 MTR_2g015580 38.329 347 153 6 268 561 45 383 6.61e-72 236
MS.gene38512.t1 MTR_2g015630 39.296 341 144 7 275 560 36 368 1.22e-71 234
MS.gene38512.t1 MTR_2g015590 37.609 343 156 7 268 558 29 365 5.21e-70 230
MS.gene38512.t1 MTR_3g466240 57.592 191 53 1 326 516 32 194 9.74e-66 213
MS.gene38512.t1 MTR_2g043990 41.319 288 92 7 249 486 77 337 1.31e-54 188
MS.gene38512.t1 MTR_3g466190 60.976 164 11 2 292 415 1 151 2.33e-52 179
MS.gene38512.t1 MTR_3g466190 93.590 78 5 0 491 568 152 229 1.37e-46 163
MS.gene38512.t1 MTR_2g015590 36.029 272 123 4 268 488 29 300 2.39e-47 169
MS.gene38512.t1 MTR_4g104240 30.523 344 175 10 267 558 36 367 4.03e-45 164
MS.gene38512.t1 MTR_7g087190 28.232 379 192 11 249 560 20 385 1.82e-39 149
MS.gene38512.t1 MTR_7g111780 31.250 336 173 12 270 560 36 358 9.44e-39 146
MS.gene38512.t1 MTR_7g116870 30.812 357 174 12 268 566 31 372 1.63e-37 143
MS.gene38512.t1 MTR_1g030220 30.362 359 166 12 268 560 38 378 4.83e-37 142
MS.gene38512.t1 MTR_8g028160 30.458 371 179 15 253 558 18 374 2.58e-36 140
MS.gene38512.t1 MTR_1g030268 31.921 354 165 13 268 560 38 376 8.26e-36 138
MS.gene38512.t1 MTR_1g030275 30.123 405 174 17 225 560 11 375 8.17e-34 132
MS.gene38512.t1 MTR_8g089805 27.095 358 182 12 268 560 38 381 1.05e-33 132
MS.gene38512.t1 MTR_1g030230 28.283 396 193 13 228 560 3 370 4.91e-33 130
MS.gene38512.t1 MTR_6g007090 29.679 374 185 13 247 560 17 372 1.77e-32 129
MS.gene38512.t1 MTR_1g030340 29.132 357 174 10 268 560 40 381 3.81e-32 128
MS.gene38512.t1 MTR_7g111780 31.933 238 144 9 330 560 33 259 4.20e-30 119
MS.gene38512.t1 MTR_7g033165 27.933 358 175 11 268 559 31 371 5.15e-30 122
MS.gene38512.t1 MTR_7g113860 26.171 363 189 13 260 558 25 372 1.28e-27 115
MS.gene38512.t1 MTR_7g033150 28.571 364 177 11 268 563 33 381 2.36e-27 114
MS.gene38512.t1 MTR_7g113860 26.966 356 183 13 266 558 25 366 3.34e-27 114
MS.gene38512.t1 MTR_1g030265 33.184 223 125 8 350 560 133 343 6.40e-27 112
MS.gene38512.t1 MTR_6g007095 28.389 391 197 14 236 564 6 375 3.95e-26 113
MS.gene38512.t1 MTR_6g007095 26.031 388 183 14 242 560 338 690 1.01e-17 87.4
MS.gene38512.t1 MTR_7g033155 27.467 375 178 11 268 563 36 395 4.69e-26 110
MS.gene38512.t1 MTR_8g089805 28.992 238 140 9 335 560 93 313 2.02e-25 107
MS.gene38512.t1 MTR_1g030268 31.136 273 128 8 268 487 38 303 5.81e-25 106
MS.gene38512.t1 MTR_8g038680 25.562 356 187 11 268 560 26 366 2.25e-24 105
MS.gene38512.t1 MTR_1g082390 25.205 365 195 14 260 561 21 370 8.97e-24 103
MS.gene38512.t1 MTR_7g113860 26.818 220 141 8 350 558 88 298 6.99e-21 94.0
MS.gene38512.t1 MTR_8g031420 26.453 344 174 14 266 554 29 348 8.33e-21 94.7
MS.gene38512.t1 MTR_6g007100 27.920 351 174 16 268 564 31 356 6.26e-20 92.0
MS.gene38512.t1 MTR_4g056280 25.419 358 191 14 247 551 10 344 4.79e-18 86.3
MS.gene38512.t1 MTR_7g033165 27.308 260 131 6 268 473 31 286 1.48e-17 84.3
MS.gene38512.t1 MTR_8g022810 26.829 328 174 13 269 546 40 351 7.80e-17 82.8
MS.gene38512.t1 MTR_8g014910 23.770 366 195 14 247 556 15 352 2.47e-16 81.3
MS.gene38512.t1 MTR_5g022630 24.024 333 170 11 270 543 43 351 9.21e-15 76.6
MS.gene38512.t1 MTR_2g015080 22.552 337 179 12 272 546 40 356 6.19e-14 73.9
MS.gene38512.t1 MTR_1g025780 27.039 233 143 8 328 552 141 354 6.29e-14 73.9
MS.gene38512.t1 MTR_8g079050 26.259 278 167 13 291 553 106 360 1.18e-13 73.2
MS.gene38512.t1 MTR_1g025720 24.925 333 176 13 268 547 35 346 2.33e-13 72.0
MS.gene38512.t1 MTR_5g083040 22.687 335 199 10 270 555 32 355 8.65e-13 70.5
MS.gene38512.t1 MTR_1g025680 25.294 340 173 15 270 552 34 349 3.97e-12 68.9
MS.gene38512.t1 MTR_5g022640 23.204 362 197 13 247 552 23 359 4.51e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38512.t1 AT5G45910 41.477 352 141 11 266 559 26 370 5.00e-77 248
MS.gene38512.t1 AT2G27360 36.919 344 158 8 268 558 31 368 5.80e-63 212
MS.gene38512.t1 AT1G28610 36.628 344 159 8 269 560 30 366 1.62e-61 208
MS.gene38512.t1 AT1G28590 35.260 346 166 7 268 561 34 373 1.73e-61 208
MS.gene38512.t1 AT1G31550 36.257 342 161 8 268 558 34 369 2.76e-61 207
MS.gene38512.t1 AT1G28600 35.393 356 172 8 255 558 15 364 6.29e-60 204
MS.gene38512.t1 AT1G31550 35.673 342 160 8 268 558 34 366 5.12e-59 201
MS.gene38512.t1 AT1G28640 34.783 345 167 8 268 560 33 371 5.81e-59 201
MS.gene38512.t1 AT1G28570 35.277 343 164 8 268 558 32 368 2.47e-57 197
MS.gene38512.t1 AT1G28670 34.682 346 167 8 268 560 33 372 4.64e-57 196
MS.gene38512.t1 AT1G28580 32.967 364 186 8 247 558 14 371 8.72e-57 196
MS.gene38512.t1 AT1G28570 36.577 298 149 6 268 558 32 296 2.83e-55 189
MS.gene38512.t1 AT1G28650 34.104 346 168 9 268 560 35 373 3.97e-55 191
MS.gene38512.t1 AT1G28660 34.104 346 168 9 268 560 33 371 7.01e-54 187
MS.gene38512.t1 AT1G28660 34.104 346 167 10 268 560 33 370 5.03e-53 185
MS.gene38512.t1 AT1G28580 35.169 236 143 4 327 558 61 290 3.43e-46 165
MS.gene38512.t1 AT3G48460 29.679 374 185 11 247 558 11 368 1.33e-43 160
MS.gene38512.t1 AT5G03980 33.968 315 171 9 255 550 14 310 4.59e-43 157
MS.gene38512.t1 AT1G28570 37.441 211 122 5 352 558 1 205 1.77e-40 147
MS.gene38512.t1 AT1G56670 31.105 344 173 12 270 565 40 367 4.75e-37 141
MS.gene38512.t1 AT4G01130 30.028 353 173 12 268 558 32 372 1.73e-34 134
MS.gene38512.t1 AT1G09390 29.651 344 178 11 270 565 37 364 1.26e-33 132
MS.gene38512.t1 AT1G54790 27.933 358 179 14 265 558 27 369 1.64e-31 126
MS.gene38512.t1 AT3G62280 27.711 332 185 12 271 558 37 357 3.26e-28 116
MS.gene38512.t1 AT1G67830 27.855 359 178 14 261 553 21 364 6.60e-28 115
MS.gene38512.t1 AT1G54790 26.184 359 194 12 260 558 21 368 1.10e-27 115
MS.gene38512.t1 AT5G14450 26.593 361 183 11 268 564 39 381 3.33e-27 114
MS.gene38512.t1 AT4G01130 29.615 260 154 9 319 558 36 286 1.49e-26 110
MS.gene38512.t1 AT1G54790 25.330 379 198 13 260 558 21 394 1.54e-26 112
MS.gene38512.t1 AT3G05180 26.346 353 181 13 268 556 34 371 5.24e-26 110
MS.gene38512.t1 AT3G26430 26.158 367 174 14 268 560 29 372 2.24e-23 102
MS.gene38512.t1 AT1G56670 30.303 264 132 7 270 487 40 297 2.59e-23 100
MS.gene38512.t1 AT3G27950 25.469 373 198 11 249 560 11 364 1.03e-21 97.8
MS.gene38512.t1 AT4G01130 27.839 273 140 7 268 488 32 299 1.91e-20 93.2
MS.gene38512.t1 AT1G06990 24.800 375 204 14 236 555 5 356 1.32e-17 85.1
MS.gene38512.t1 AT1G75920 26.667 315 165 14 268 552 27 305 3.55e-15 77.0
MS.gene38512.t1 AT2G23540 26.667 345 171 16 270 553 48 371 3.59e-13 72.0
MS.gene38512.t1 AT1G23500 22.754 334 181 11 269 553 34 339 1.43e-12 69.7
MS.gene38512.t1 AT1G20132 25.145 346 176 13 270 557 61 381 2.02e-12 69.7

Find 114 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATGTCTTAGTGTCTTTA+TGG 0.130396 3.4:-61834617 None:intergenic
ATCCTTCGAAACTAATAAAT+TGG 0.141859 3.4:+61834919 MS.gene38512:CDS
TCGGTAGAGTTGGTGGTTTC+AGG 0.166299 3.4:+61834460 MS.gene38512:CDS
ACAAAACTGTTGAAGCTTTC+AGG 0.177894 3.4:-61827338 None:intergenic
AAAGAAGACTATGATAAATT+TGG 0.244489 3.4:+61834538 MS.gene38512:CDS
AAGAAGCCTAATAAGTTTAA+AGG 0.253041 3.4:+61827503 MS.gene38512:CDS
ATGGCTGTAGGAAAACTTTA+TGG 0.267852 3.4:+61833549 MS.gene38512:CDS
CCATTTCCTTTAAACTTATT+AGG 0.276217 3.4:-61827509 None:intergenic
GGCTTCAAAGCTTTGGTTGA+TGG 0.304319 3.4:-61827554 None:intergenic
GCGTAACTGCCCAAAGTATC+TGG 0.312221 3.4:+61827637 MS.gene38512:CDS
ATTCCATATGCTTCAGCAAT+TGG 0.318041 3.4:+61830119 MS.gene38512:CDS
CCTAATAAGTTTAAAGGAAA+TGG 0.333520 3.4:+61827509 MS.gene38512:CDS
GTTGTCAAAAGCAAGATTTC+CGG 0.336765 3.4:-61833517 None:intergenic
CACAGAAATGATCATCAAAA+TGG 0.340158 3.4:-61834869 None:intergenic
TCGATATGATCGGACATCTA+AGG 0.342232 3.4:+61827126 MS.gene38512:CDS
CAAAGCTTTGGTTGATGGCT+TGG 0.343191 3.4:-61827549 None:intergenic
TCCAGTAGGCTTCAAAGCTT+TGG 0.351131 3.4:-61827561 None:intergenic
TCTTGTCTTCCAGATACTTT+GGG 0.352563 3.4:-61827646 None:intergenic
TTCTTGTCTTCCAGATACTT+TGG 0.353539 3.4:-61827647 None:intergenic
TGTCATTCATGAGGAAGTTC+TGG 0.355932 3.4:-61827361 None:intergenic
CCTTGAATATGCAATTTGAT+TGG 0.379794 3.4:+61834012 MS.gene38512:CDS
TTTAATTGATTCAACAATCA+AGG 0.383921 3.4:-61834279 None:intergenic
CTGACAGCTTGGTGGAGAAA+AGG 0.395925 3.4:-61827199 None:intergenic
TCCCTTTCTTCGTTAGTTGA+AGG 0.396037 3.4:-61830083 None:intergenic
TACCAAGCACCACAACAATA+TGG 0.406934 3.4:+61834694 MS.gene38512:CDS
CCAATCAAATTGCATATTCA+AGG 0.407925 3.4:-61834012 None:intergenic
CTCTGAGAACCTAGCTCGAT+TGG 0.411708 3.4:+61827265 MS.gene38512:CDS
CCTCATGCACTCAAGATGAT+TGG 0.417681 3.4:+61827239 MS.gene38512:CDS
GACCCAATTGCTGAAGCATA+TGG 0.419035 3.4:-61830122 None:intergenic
GTTTCAGGGAACTTTCCAAT+AGG 0.428199 3.4:+61834475 MS.gene38512:CDS
TTCCATATGCTTCAGCAATT+GGG 0.428710 3.4:+61830120 MS.gene38512:CDS
CTCTTTATGAAGAGCAAGCC+AGG 0.435723 3.4:+61827150 MS.gene38512:CDS
TATTTGTTTATGAGCTGCTT+CGG 0.437249 3.4:-61834958 None:intergenic
AACGTACTTTAAACATTCAT+CGG 0.439640 3.4:+61833575 MS.gene38512:CDS
CGACTCATCATAGATTTCAT+AGG 0.447348 3.4:+61833615 MS.gene38512:CDS
TGACAGCTTGGTGGAGAAAA+GGG 0.449009 3.4:-61827198 None:intergenic
GCCATTATTAACCATTAAAA+CGG 0.449060 3.4:-61827462 None:intergenic
CCCCTTCAACTAACGAAGAA+AGG 0.449286 3.4:+61830081 MS.gene38512:CDS
TCCTTCGAAACTAATAAATT+GGG 0.449302 3.4:+61834920 MS.gene38512:CDS
CCCTTTCTTCGTTAGTTGAA+GGG 0.453626 3.4:-61830082 None:intergenic
CGGTAGAGTTGGTGGTTTCA+GGG 0.454891 3.4:+61834461 MS.gene38512:CDS
GTGCTTCTACTGCAACAAGC+AGG 0.455787 3.4:+61827604 MS.gene38512:CDS
ACCAAAGCTTTGAAGCCTAC+TGG 0.461471 3.4:+61827560 MS.gene38512:CDS
CACCATATTGTTGTGGTGCT+TGG 0.462350 3.4:-61834696 None:intergenic
AATATATTATCTTAGCTTGA+GGG 0.464764 3.4:-61834642 None:intergenic
TCACAGGCTTTAATTGAAGA+AGG 0.465958 3.4:+61834436 MS.gene38512:intron
TTGTTTATAGTGGGAGAGAT+TGG 0.468383 3.4:+61834197 MS.gene38512:CDS
TACTGGAGGCGTGGCTAAGG+AGG 0.473165 3.4:+61827577 MS.gene38512:CDS
AAGTGAGCAGTACACCCTAT+TGG 0.473926 3.4:-61834490 None:intergenic
CTCATGCACTCAAGATGATT+GGG 0.477686 3.4:+61827240 MS.gene38512:CDS
TTTCAGGGAACTTTCCAATA+GGG 0.477863 3.4:+61834476 MS.gene38512:CDS
TCCCAATTTATTAGTTTCGA+AGG 0.478073 3.4:-61834921 None:intergenic
ATTGGGATTTGTCCTTGAGC+AGG 0.479117 3.4:+61827283 MS.gene38512:CDS
TAAACTATCTTGAAAGGAGC+GGG 0.479309 3.4:-61835026 None:intergenic
GCCTACTGGAGGCGTGGCTA+AGG 0.485232 3.4:+61827574 MS.gene38512:CDS
AATGTTCCATCCACTTCTAC+AGG 0.486155 3.4:+61827677 MS.gene38512:CDS
ACTGCAACAAGCAGGGGCAT+TGG 0.486440 3.4:+61827612 MS.gene38512:CDS
CTAAACTATCTTGAAAGGAG+CGG 0.486604 3.4:-61835027 None:intergenic
TGAAGAAGGATCGGTAGAGT+TGG 0.489354 3.4:+61834450 MS.gene38512:CDS
AGAAGGATCGGTAGAGTTGG+TGG 0.489394 3.4:+61834453 MS.gene38512:CDS
GACATCAAACCTTTCATGCC+TGG 0.489946 3.4:-61827168 None:intergenic
TCTGAGAACCTAGCTCGATT+GGG 0.497824 3.4:+61827266 MS.gene38512:CDS
ACGTACTTTAAACATTCATC+GGG 0.505288 3.4:+61833576 MS.gene38512:CDS
CTTGAGTGCATGAGGTCCTA+CGG 0.506771 3.4:-61827231 None:intergenic
TCCTTGTGTAAAACTAAAGA+AGG 0.509172 3.4:+61834053 MS.gene38512:CDS
ACTTCCTTCTGACAGCTTGG+TGG 0.509493 3.4:-61827207 None:intergenic
AAGGCATGTTGTGGAGGAAG+TGG 0.510426 3.4:+61834840 MS.gene38512:CDS
AAGCTGAAGATGGCTTCATA+TGG 0.511676 3.4:-61833474 None:intergenic
CCCTTCAACTAACGAAGAAA+GGG 0.513833 3.4:+61830082 MS.gene38512:CDS
TTAATTGATTCAACAATCAA+GGG 0.514350 3.4:-61834278 None:intergenic
TATGAAGCCATCTTCAGCTT+CGG 0.518684 3.4:+61833477 MS.gene38512:CDS
CTTAATGCATACGAGACATC+TGG 0.520103 3.4:-61830173 None:intergenic
GGGACTTCCTTCTGACAGCT+TGG 0.521173 3.4:-61827210 None:intergenic
CCTTTCTTCGTTAGTTGAAG+GGG 0.521679 3.4:-61830081 None:intergenic
TATCTGGAAGACAAGAAGAA+TGG 0.523238 3.4:+61827653 MS.gene38512:CDS
GCTCATAAACAAATAACTAA+AGG 0.524203 3.4:+61834966 MS.gene38512:CDS
TTCTCCACCAAGCTGTCAGA+AGG 0.524725 3.4:+61827203 MS.gene38512:CDS
GGCTTTAATTGAAGAAGGAT+CGG 0.533068 3.4:+61834441 MS.gene38512:intron
TTATAGTGGGAGAGATTGGT+AGG 0.533892 3.4:+61834201 MS.gene38512:CDS
AAATGATCATCAAAATGGTA+TGG 0.536075 3.4:-61834864 None:intergenic
AAGAGCAAGCCAGGCATGAA+AGG 0.540100 3.4:+61827159 MS.gene38512:CDS
TGCTTCTACTGCAACAAGCA+GGG 0.541693 3.4:+61827605 MS.gene38512:CDS
AAATATATTATCTTAGCTTG+AGG 0.542778 3.4:-61834643 None:intergenic
TTGAGTGCATGAGGTCCTAC+GGG 0.546529 3.4:-61827230 None:intergenic
TTGATGATCATTTCTGTGGA+TGG 0.551682 3.4:+61834874 MS.gene38512:CDS
CTTAACATTGCTGCAAGCTG+TGG 0.559764 3.4:-61827398 None:intergenic
TCCTTAGCCACGCCTCCAGT+AGG 0.563586 3.4:-61827575 None:intergenic
AGAGTCACCGAAGCTGAAGA+TGG 0.567715 3.4:-61833484 None:intergenic
GGACAAATCCCAATCGAGCT+AGG 0.567817 3.4:-61827274 None:intergenic
CAATTCTAAACTATCTTGAA+AGG 0.577144 3.4:-61835032 None:intergenic
TCATCGGGACGTTTATCAGA+TGG 0.578128 3.4:+61833591 MS.gene38512:CDS
TCGAAACTAATAAATTGGGA+TGG 0.581957 3.4:+61834924 MS.gene38512:CDS
CTCCACGCACCATATTGTTG+TGG 0.584935 3.4:-61834703 None:intergenic
ATAACTAAAGGTTTAATCGA+AGG 0.587262 3.4:+61834978 MS.gene38512:CDS
AAAAGCAAGATTTCCGGTGT+CGG 0.587575 3.4:-61833511 None:intergenic
AAAATACCTGTAGAAGTGGA+TGG 0.587914 3.4:-61827683 None:intergenic
ATTAGTATCACCTTTGCATG+TGG 0.589824 3.4:+61833414 MS.gene38512:intron
TTTGGTATAACATACATTGG+TGG 0.589894 3.4:-61834306 None:intergenic
ACTGGAGGCGTGGCTAAGGA+GGG 0.597145 3.4:+61827578 MS.gene38512:CDS
GCTTCTACTGCAACAAGCAG+GGG 0.602179 3.4:+61827606 MS.gene38512:CDS
CAACAACAAGTTCCTGCTCA+AGG 0.605424 3.4:-61827295 None:intergenic
AAACATGAAGGGAAAGGGAA+AGG 0.606413 3.4:+61827433 MS.gene38512:CDS
CCAATCATCTTGAGTGCATG+AGG 0.611352 3.4:-61827239 None:intergenic
TATTACAGAACTGCGAGAGA+TGG 0.617839 3.4:+61834253 MS.gene38512:CDS
TGAGCTGCTTCGGTAAAGTG+TGG 0.626679 3.4:-61834948 None:intergenic
AAGTTTAAAGGAAATGGCAA+AGG 0.629410 3.4:+61827515 MS.gene38512:CDS
CACCACAACAATATGGTGCG+TGG 0.629799 3.4:+61834701 MS.gene38512:CDS
ATGACTGCGTTCGATATGAT+CGG 0.633331 3.4:+61827116 MS.gene38512:CDS
TTTGAAGCCTACTGGAGGCG+TGG 0.635827 3.4:+61827568 MS.gene38512:CDS
GAACTTCCTCATGAATGACA+TGG 0.647022 3.4:+61827364 MS.gene38512:CDS
CTGTCAGAAGGAAGTCCCGT+AGG 0.658423 3.4:+61827215 MS.gene38512:CDS
AACAAGCCACCTATGGCTGT+AGG 0.672703 3.4:+61833537 MS.gene38512:CDS
GGTGACTCTATAACCGACAC+CGG 0.692180 3.4:+61833498 MS.gene38512:CDS
AAAGCTTTGAAGCCTACTGG+AGG 0.718217 3.4:+61827563 MS.gene38512:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.4 gene 61827083 61835049 61827083 ID=MS.gene38512
chr3.4 mRNA 61827083 61835049 61827083 ID=MS.gene38512.t1;Parent=MS.gene38512
chr3.4 exon 61827083 61827698 61827083 ID=MS.gene38512.t1.exon1;Parent=MS.gene38512.t1
chr3.4 CDS 61827083 61827698 61827083 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
chr3.4 exon 61830057 61830183 61830057 ID=MS.gene38512.t1.exon2;Parent=MS.gene38512.t1
chr3.4 CDS 61830057 61830183 61830057 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
chr3.4 exon 61833419 61833636 61833419 ID=MS.gene38512.t1.exon3;Parent=MS.gene38512.t1
chr3.4 CDS 61833419 61833636 61833419 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
chr3.4 exon 61833994 61834074 61833994 ID=MS.gene38512.t1.exon4;Parent=MS.gene38512.t1
chr3.4 CDS 61833994 61834074 61833994 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
chr3.4 exon 61834171 61834313 61834171 ID=MS.gene38512.t1.exon5;Parent=MS.gene38512.t1
chr3.4 CDS 61834171 61834313 61834171 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
chr3.4 exon 61834442 61834724 61834442 ID=MS.gene38512.t1.exon6;Parent=MS.gene38512.t1
chr3.4 CDS 61834442 61834724 61834442 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
chr3.4 exon 61834811 61835049 61834811 ID=MS.gene38512.t1.exon7;Parent=MS.gene38512.t1
chr3.4 CDS 61834811 61835049 61834811 ID=cds.MS.gene38512.t1;Parent=MS.gene38512.t1
Gene Sequence

>MS.gene38512

ATGACTCCCGAGCTGCAAAAACAGCATGAAGGGATGACTGCGTTCGATATGATCGGACATCTAAGGACTCTTTATGAAGAGCAAGCCAGGCATGAAAGGTTTGATGTCTCTAAATCCCTTTTCTCCACCAAGCTGTCAGAAGGAAGTCCCGTAGGACCTCATGCACTCAAGATGATTGGGTACTCTGAGAACCTAGCTCGATTGGGATTTGTCCTTGAGCAGGAACTTGTTGTTGATCTTGTCTTGCAATCGTTGCCTGAAAGCTTCAACAGTTTTGTCCAGAACTTCCTCATGAATGACATGGACAAAACACTTCCACAGCTTGCAGCAATGTTAAGAACTGCTGAGAAAAACATGAAGGGAAAGGGAAAGGCTGCTGCCGTTTTAATGGTTAATAATGGCAAGTTCAAAAAGCAAAATAAGAAGCCTAATAAGTTTAAAGGAAATGGCAAAGGCAAAGCTGTTGCCAAGCCATCAACCAAAGCTTTGAAGCCTACTGGAGGCGTGGCTAAGGAGGGCAAGTGCTTCTACTGCAACAAGCAGGGGCATTGGAAGCGTAACTGCCCAAAGTATCTGGAAGACAAGAAGAATGGAAATGTTCCATCCACTTCTACAGGTATTTTTGTTATAGAAATTAATATGTCTACTTCTACATCATGGGTATTAGATACTGGATGTGGTTCTCATATTTGTACTGATGTGCAGGGGCTGAGAATGAGTAGAGCATTGGCAAAAGGCGAGGTCGATCTTAGAGTTGGCAATGGAGCAAAGGTTGCTGCTTTGGCTGTTGGAACTTATGTATTGACCTTACCTAGTGGTCTTTTACTTAATTTGCAAAACTGTTATTATGTACCTGCCATTAGCAGGAATATCATTTCAATTTCTTGTTTGGACAAGGCTGGTTTTTATTTTACAATTAAGGACAAATGTTGTTCTGTTTATTTGGATAATATTATCTATGCTAATGCAAATTTGAGTAATGGACTTTATATCCTAGATCTTGATATACCTATCTATAACATTAATACTAAAAGGATGAAACCTAATGAGTTAAATCCGACTTACCTTTGGCATTGTCGTTTAGGACATATAAATGAGAATCGCATTTCCAAACTCCATAAAGATGGATTTTTGGACTCTTTTGATTTTGAATCATATGAAACATGTCGATCTTGTTTGTTAGGAAAGATGACGAAGACTCCATTTACCGGCCAAGGTGAAAGGGCTAGTGATCTTTTAGGTCTTATACATACTGATGTATGTGGACCTCTAAACATAACTGCTAGAGGAGGTTTTCACTACTTCATCACATTCACTGATGACTTTAGTAGATTTGGATATGTGTATCTAATGAAACATAAATCTGAATCATTTACTTTATTCAAGGAATTTCAAAATGAAGTAGAAAACCAACTAGGCAAGAAAATCAAAATGCTTCGATCTGATCGAGGTGGAGAGTATTTGAGCTTAGAGTTTGACAATCATCTAAAAGAATGTGGGATCTTGTCCCAACTTACACCTCCTGGAACACCACAATGGAATGGTGTTTCTGAAAGGAGAAATCGAACCTTGTTAGACATGGTCCGATCAATGATGAGTCATGCTGAACTTCCAAACACCTTATGGGGATACGCCTTATTAACTGCAGCTTATACACTTAACCGAGTTCCATCTAAAGCGGTAGAAAAGACACCATATGAGATATGGAATGGTCGAAAACCCAATGTGGGACATTTAAAGATTTGGGGTTGTGAAGCTTATATAAAACGACTTATGTCTACTAAGCTTGAACAAAGATCTGACAAATGTTTCTTTGTGGGATATCCTAAAGAAACTAGAGGATACTACTTTTACAAACCTTCTGAGGGAACAATTGTTGTTGCCAGAAACGGAGTCTTCCTTGAGAAGGATTTTGTCTCCAAAAGAATCAGTGGGAGCAAAGTAGATCTTGAAGAAGTTCAAGACCCTCAAAGAGATAACATACCGATGGAGGAACAGGAACAGGATACACAAACTGTTGTGACAGAAAATCCTATTCCAGTAACACAAGAACCACGTAGGTCTAACAGGATACATCAAGAACCCGAAAGATATGGGTATCTCATATCAAAAGAGGGTGATGTATTGCTCATGGACCAAGATGAGCCTGTTACTTACACAGAGGCCATTACTGGTCCTGAATCTGAGAAGTGGCTTGAAGCCATGAAATCTGAAATGGATTCCATGTACACAAATCAAGTTTGGAACTTGATTGAAGCACCTGAAGGAATTAATCCTATAGGATGCAAGTGGATTTTCAAAAAGAAAATTGACATGGATGGAAAGGTTAATACCTACAAAGCACGATTGGTTGCTAAAGGCTTCAAACAAATTCATGGTATAGACTATGATGAAACCTTTTCACCAGTTGCAATGATTAAATCCATTCGGATATTACTTGCTATCGCTGCATACCATGATTATGAAATCTGGCAAATGGATGTCAAAACTGCTTTCCTTAATGGAAATCTTCTTGAGGATGTGTACATGACACAACCTGAAGGATTTGGCGATCCTAAAGCATCAAAGAAAGTATGTAAGTTACAGCGATCAATCTACGGATTAAAGCAAGCTTCCAGAAGTTGGAATCTTCGTTTTGATGAAACTGTACAACAGTATGGATTCATTAAAAATGAAGATGAACCTTGTGTTTACAAGAAGGTTAGTGGGAGCATAATCTCATTTCTGGTTTTATATGTGGATGACATATTGTTAATCGGAAATGACATCCCTACATTACAAGGAATCAAAACTTGGTTAGGGAAATGCTTTTCTATGAAAGACTTAGGTGAAGCCTCCTATATATTAGGAATTAGGATCTATAGAGATAGATCACAAAGATTGCTTGGCTTAAGTCAGGGTACATATATAGACAAAGTCCTGAGACGTTTCAATATGCATGATTCCAAGAAAGGATTCATCCCTATGTCGTCAGGTATAAATCTATCAAAGAATCAATCCCCTTCAACTAACGAAGAAAGGGATCGAATGCGTGATATTCCATATGCTTCAGCAATTGGGTCTATCATGTATGCTATGATATGTACTCGACCAGATGTCTCGTATGCATTAAGTGCTACGAGCAGATACCAGTCTAATCCTGGCAATGATCATTGGATTGCCGTCAAGAATATCCTTAAGTACTTGAGAAGAACTAAGGATACTTTCTTGATCTATGGAGGTCAAGAAGAGCTCTCTGTAATCGGTTACACTGATGCTAGTTTTCAGACAGATCATGATGATTTTAAATCGCAATCTGGATATGTGTTTTGCTTAAATGGCGGTGCTGTGAGCTGGAAGAGTTCGAAGCAAGAAACAGTCGCTGATTCTACAACAGAAGCTGAGTATATTGCTGCATCTAATGCCGCAAAGGAAGCTGTTTGGATTAAGAAATTTATTTCTGATCTTGGAATAGTTCCAAATATTGTGGATCCCATTGAATTACTATGTGATAACAATGGGGCAATCGCACAAGCTAAAGAACCTAGATCTCACCAGAAATCCAAACACATACAAAGGCGCTATCATCTCATTCGAGAGATTATCGAAAGAGGAGATGTTAAAATATGCAAAGTACCAACATTAGAGAATGTTGCTGATCCACTTACTAAAGCTCTAGCCCAGCAGAAGCATGATGGTCATACTAGATCTATGGGCATTAGGTTTATGCCTAATTGGCTCTAGTGCTAGTGGGAGATTGTTGGTGTAAGCCCTAGAGGCCAATAGTTTATGTTAAACTTATATTATGTATCATGACTTTTATTATTGAATAAAATTGGCACTCTTTATTATATTTATATATTGTTAATAAAGTCCTTAGAATAGATAGTTCATGTAATAAATATATTAAGTGAGACTTAATCATGAGATTACATTATCGTAAAGAACGCTATTCTTAAACGTATCCATAGTCAAAGCTTTAATATGAATAAAGGATAATAATAAAGCGTCGAGACTATTATGTATGTAGACTGATGACCGCATCTCATGGGTCATAGATATGAGATATTGAGTCTATACATAGGTATAAATACTAGGAGTAATATTTATACTGGATTGACCCACCGTGAGAATACTACATAGTATGTTATGATCATGTCGTAAGATATTCTCGCAATGATAATAGTGTATATCGCTCTTAGACCTGAAACCACTATGATCCCTAGATGTGGACTCAAGTGCTTTATTGCCATTCTAACGTTGTCTGTAAAAAGATAACCATAACGTTGGTTGATGGGTACTTGATGAATCATGCTGAGGGACATGAGTGTCCTAGATGGGATTTGTCCCTCCTCATAAACAGGAGATATATCTTTAGGCCTCTTGATGAAATACAACTGTAAAACTGCATGGCCATGCCGAACTAAGTCAATATAAGATATTGGACTTATTCATTATTCAGTATATTTCGGAATCAAGAAATGTAAACATTGATCTATACAAGGATGACAATATCTATGCCTTGGGATCAATATAGATATAGAGGCAAAGGAGTACTTGTACACATAAGTATTATCATGGAAAAGGTTTCGTCAGATCACTTGACATTCTTGTCACTTGGGTAGCAATGATGTGTTGCTAGATACCGCTCATTGTTTATAATATTAAATATAGGATTTAATATTATTGCCAACGTAACTTGAACCTATGGGGTCACACACAAAGAACGGTCAAAAGAGAAATATAAGTAAGACTTATATGATGGGTGCATTTAGTAATTAACGCTAATTAGTTAGCAAATGACTGAATTCGTTATTGGGCTTAAGGAAGCCCATTAGTGACTTAAGGCCTGCAAGAGTTCTATATATGGAACCATCATGATGGAGCTAGAGGCAAGCTTTTTTTTTGCAGAAACCCTAGTTCCTTGCAGCCACCCAATTCTCTGCAGCTTTTGATTCTTTGGCTAGCACCAAGATTGAGAGGAGCACCGCTCGTGTGGACTTGCTAGAGGCTTTGCTGCTTGCTGCTTGCTTTGCTGTGATCGTGATTCCGGTTTCCAGTTTCCAGTTTTCAGATTCCACAGCTAGAGGTAACAATCGAATCACTGATTCATGCTTGAATTCGTAATGATCTAAGGAAAGAAAATCGGAAATTTTATCCGCTGCATGTTCTGTTAAGGATCCGATTTTCCTTCAGGATCTAGCACGTGCGCCTTCCAGAGAGAAACATGACTTAAATTACGCACACTATGAAATATTTTTTAAGAATATCACAATTGAAGCTACTTAGGGTTTAAGCTACTTAGGGCATTATAAAAAAGAGAAACAAAAACTATCATACACAATTGAAGAATATCACCCAAGAATTGCTTGGAAAAAGTGAAGATGACCCAAAATCACATATTTATACACACAATGATCATTAAATAAAACAATAATAAAGTAAGAAAGAGTAAAAAAAAATAAAGAAGATTAAGACATATGTGAAGAGTAAGAAAGTGAAACAAAAACTATCATAGATGAAAAGTATCACCTAAGAATTACTTAGGGAAAGTGAAAATGACCCCAAATCACATATTTATATACACAAGGATCATAAAATAAGACAATAATATAGTATAAATTCTCCTCCAAATCTTCCTCGCCCTTGTAAGATGAGCAACCATATACTCTTCCATGTTCTTCAAAGTTATTATAGTATAAATTTACTATGGTTGATTAAATAAGGTGAATGAAAGGGTGAAGTCAATTAATTAAAGAATTTTTTGTTTGGTATAAGTTAATTAACAAATTTTAATAGAATAGTGAAAAATGAAAACCGATCAATTCAAGAAATTGCAATAGACGCAAATAATAAAAATTTAAGATAAAACCAAGTTAATATATCCACCCATGCATAACACAAAAAAACGACAACGGGGGTTTTAGAAAAGCTTGGCGCATTTATATCCCAGCTTGTTGTTATTATCTCTTTTATTTATGGAAGAAGCCAAAGCGTTCATCAACATATTGAATACATATATTTATGTTATTTTAATTTAATGGGCAATTCAGTTAGTTTCTTCAAGGGGTTTTAAACTTCTTTCAATATAAATTTGTAAAATTTAACCATAATTACTTTGGATGTTTAATGAGAGATGAGGAATTTCTCTAAAGCATCACACATCAGAACAACAATGGAAGCTTAGAAACAACTCAAGGTTCGATTACAAAGATATATATATAGATATATAGATAGATAGATAAATGAGTAATTGTGAAAGTATATAAATACTATAATCTCTTTTTCTAGCAAACATCAATATAAGTTCTTTGCTATATTATCTTTTGACACAATGAAGATCTTCTTTCTCATTAGTATCACCTTTGCATGTGGCATTTTTGAAAATTTTGTTTCAAATGCTAATCTTCTACCATATGAAGCCATCTTCAGCTTCGGTGACTCTATAACCGACACCGGAAATCTTGCTTTTGACAACAAGCCACCTATGGCTGTAGGAAAACTTTATGGATCAACGTACTTTAAACATTCATCGGGACGTTTATCAGATGGACGACTCATCATAGATTTCATAGGTAGTTTTACACATGTTTTTTGTTAAATAATTTTTTATCGTATTCATGAAATTGTAAATAACAAAAGCAAATAAATAAAAAGAACTCTAACATAATATATTTTAAATAGTTGTTATAATTTTTGTCTTTTTTTGAGAAAACATAGTTGTTATAATTAAAATCAATAAATTTTAATGTTGATACATTTCTTATTGAAAAGTTCACTAAGTCGTTGTCAATTTAATTGTAGCCGAGGCGTATGGATTGCCATTTTTACCGCCCTACAAAAATATCACCAAAAGTCAAGTTGATGTAAAAAAAGGGAGTTAATTTTTGCTTATGGTGGTGCAAGTGCACTTGATGTCAATTATTTCAAAGGTGGTAGAGTCACTAACTCCTTGAATATGCAATTTGATTGGTTTAAGAAGCTAAAACCCTCCTTGTGTAAAACTAAAGAAGGTTTTGTCCTAACTCCTTGCATATTATTTTTGTGTGAAATAAATTGTTTTTCGTATATATGACTAACAAAACATTTGTTTTGTTCTGAATTCACAGAATGTGATAGCTTCTTCAAAAAATCGTTGTTTATAGTGGGAGAGATTGGTAGGAATGATATTTTATTCGATCTTTCTAAAACTATTACAGAACTGCGAGAGATGGTTCCCTTGATTGTTGAATCAATTAAAAATACCACCAATGTATGTTATACCAAATTTAAGCAATTAAATTTTTTCTTATTATCAAAATAAAATTAATAAAATGTTTACTTCATTTGTAGAGTTGGTGGAAAAATGTAATAGTCATTTATTTAATTTTTTTTTCACAGGCTTTAATTGAAGAAGGATCGGTAGAGTTGGTGGTTTCAGGGAACTTTCCAATAGGGTGTACTGCTCACTTATTGTCAAATAAGATTAGTCAGAAGAAAGAAGACTATGATAAATTTGGATGTTTGATAGCTTACAACTCTTTCATAAAATACTTTAATAAAAAACTTAGAAAATCCATAAAGACACTAAGACATAAACACCCTCAAGCTAAGATAATATATTTTGACTTTTACAATGATGTCAAACGTATATACCAAGCACCACAACAATATGGTGCGTGGAGTAATTATTTTCTTTCTTGGTTTACATTGACCTATTCTTGTCAAATTTATTTTTTCGCATGCACTAACTTTATATCAATAATGCAGGTTTTACTTCTGATAAGGTCGAGATTTTGAAGGCATGTTGTGGAGGAAGTGGACCATACCATTTTGATGATCATTTCTGTGGATGGCAGAGTACAACAGTTTGCTCTGATCCTTCGAAACTAATAAATTGGGATGGACCACACTTTACCGAAGCAGCTCATAAACAAATAACTAAAGGTTTAATCGAAGGCTCTTTTGCAAATCCTTCTCTAAAACCCGCTCCTTTCAAGATAGTTTAG
Protein sequence

>MS.gene38512.t1

MTPELQKQHEGMTAFDMIGHLRTLYEEQARHERFDVSKSLFSTKLSEGSPVGPHALKMIGYSENLARLGFVLEQELVVDLVLQSLPESFNSFVQNFLMNDMDKTLPQLAAMLRTAEKNMKGKGKAAAVLMVNNGKFKKQNKKPNKFKGNGKGKAVAKPSTKALKPTGGVAKEGKCFYCNKQGHWKRNCPKYLEDKKNGNVPSTSTGINLSKNQSPSTNEERDRMRDIPYASAIGSIMYAMICTRPDVSITFACGIFENFVSNANLLPYEAIFSFGDSITDTGNLAFDNKPPMAVGKLYGSTYFKHSSGRLSDGRLIIDFIGGRVTNSLNMQFDWFKKLKPSLCKTKEECDSFFKKSLFIVGEIGRNDILFDLSKTITELREMVPLIVESIKNTTNALIEEGSVELVVSGNFPIGCTAHLLSNKISQKKEDYDKFGCLIAYNSFIKYFNKKLRKSIKTLRHKHPQAKIIYFDFYNDVKRIYQAPQQYGAWSFTSDKVEILKACCGGSGPYHFDDHFCGWQSTTVCSDPSKLINWDGPHFTEAAHKQITKGLIEGSFANPSLKPAPFKIV