Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38541.t1 | XP_003599280.1 | 94.1 | 170 | 10 | 0 | 1 | 170 | 1 | 170 | 4.80E-84 | 320.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38541.t1 | G7IWW8 | 94.1 | 170 | 10 | 0 | 1 | 170 | 1 | 170 | 3.5e-84 | 320.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38541 | MS.gene004251 | PPI |
| MS.gene38541 | MS.gene54297 | PPI |
| MS.gene38541 | MS.gene002580 | PPI |
| MS.gene38541 | MS.gene01564 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38541.t1 | MTR_3g031140 | 97.647 | 170 | 4 | 0 | 1 | 170 | 1 | 170 | 2.40e-121 | 339 |
| MS.gene38541.t1 | MTR_5g074290 | 65.116 | 172 | 44 | 5 | 1 | 170 | 1 | 158 | 2.99e-59 | 182 |
| MS.gene38541.t1 | MTR_2g016150 | 42.473 | 186 | 84 | 4 | 1 | 169 | 1 | 180 | 1.37e-31 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38541.t1 | AT2G30230 | 54.891 | 184 | 60 | 3 | 1 | 170 | 1 | 175 | 5.05e-58 | 179 |
| MS.gene38541.t1 | AT1G06980 | 55.056 | 178 | 61 | 2 | 1 | 170 | 1 | 167 | 5.53e-56 | 174 |
| MS.gene38541.t1 | AT1G29195 | 43.386 | 189 | 86 | 5 | 1 | 169 | 1 | 188 | 4.68e-44 | 144 |
Find 42 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAACAACGCCTTGAGAACT+TGG | 0.264820 | 3.4:-61398576 | None:intergenic |
| AGAAAGGTCGTGTTGGAATA+TGG | 0.306669 | 3.4:+61398874 | MS.gene38541:CDS |
| AACATCACCCTTGCAATATA+AGG | 0.321491 | 3.4:+61398824 | MS.gene38541:CDS |
| TGGCTTGTGTTCTTCCTTGT+GGG | 0.333742 | 3.4:+61398442 | MS.gene38541:CDS |
| TAATAGCTTAAGGTGTTGTT+TGG | 0.366641 | 3.4:+61398422 | MS.gene38541:CDS |
| GGTTTGCTTAGAACATGGTT+AGG | 0.379565 | 3.4:-61398555 | None:intergenic |
| AGCCATTCAAATGCACTATT+CGG | 0.415539 | 3.4:-61398479 | None:intergenic |
| GATCCCGGCAACATCTTTGC+CGG | 0.428218 | 3.4:+61398656 | MS.gene38541:CDS |
| GTTTGCTTAGAACATGGTTA+GGG | 0.435444 | 3.4:-61398554 | None:intergenic |
| GAACATGGTTAGGGTTTGCT+TGG | 0.435589 | 3.4:-61398545 | None:intergenic |
| TTAATATGGGTAATAGCTTA+AGG | 0.438428 | 3.4:+61398412 | None:intergenic |
| CATCTTTGCCGGAGAACAAA+AGG | 0.452972 | 3.4:+61398667 | MS.gene38541:CDS |
| TTCTCCGGCAAAGATGTTGC+CGG | 0.464422 | 3.4:-61398660 | None:intergenic |
| TCAAGACGGGATCGCAAGAA+AGG | 0.467249 | 3.4:+61398858 | MS.gene38541:CDS |
| GATGGTGATCATGACAATAA+TGG | 0.475725 | 3.4:+61398771 | MS.gene38541:CDS |
| TTTCTTCTCCTTATATTGCA+AGG | 0.484846 | 3.4:-61398832 | None:intergenic |
| TTGGCTTGTGTTCTTCCTTG+TGG | 0.486689 | 3.4:+61398441 | MS.gene38541:CDS |
| GTTCTCAAGGCGTTGTTCGT+CGG | 0.495768 | 3.4:+61398580 | MS.gene38541:CDS |
| ATCACCATCATCACCGTGCT+TGG | 0.502755 | 3.4:-61398757 | None:intergenic |
| AGCACCAAGCACGGTGATGA+TGG | 0.505783 | 3.4:+61398753 | MS.gene38541:CDS |
| TTCTTCTCCTTATATTGCAA+GGG | 0.509390 | 3.4:-61398831 | None:intergenic |
| CCTCTCTTGAGCTCAGTCTC+CGG | 0.511219 | 3.4:-61398618 | None:intergenic |
| ATCCGAATAGTGCATTTGAA+TGG | 0.519987 | 3.4:+61398477 | MS.gene38541:CDS |
| GGCTTGTGTTCTTCCTTGTG+GGG | 0.520338 | 3.4:+61398443 | MS.gene38541:CDS |
| GACAATAATGGTAGTGGTGA+TGG | 0.523601 | 3.4:+61398783 | MS.gene38541:CDS |
| TCTCCGGCAAAGATGTTGCC+GGG | 0.532431 | 3.4:-61398659 | None:intergenic |
| GGAGAAGAAATCCTCAAGAC+GGG | 0.545214 | 3.4:+61398845 | MS.gene38541:CDS |
| TTTCTTGCGATCCCGTCTTG+AGG | 0.547201 | 3.4:-61398856 | None:intergenic |
| TCAGAAATACTTGCTAAATG+AGG | 0.557315 | 3.4:-61398900 | None:intergenic |
| TTGCCGGAGAACAAAAGGAA+TGG | 0.573260 | 3.4:+61398672 | MS.gene38541:CDS |
| CTAAGCAAACCAAGTTCTCA+AGG | 0.584166 | 3.4:+61398567 | MS.gene38541:CDS |
| AAGCATATACTTCTTGATCC+CGG | 0.589215 | 3.4:+61398641 | MS.gene38541:CDS |
| AGGAGAAGAAATCCTCAAGA+CGG | 0.590285 | 3.4:+61398844 | MS.gene38541:CDS |
| AACTTGGTTTGCTTAGAACA+TGG | 0.593833 | 3.4:-61398560 | None:intergenic |
| GATCATGACAATAATGGTAG+TGG | 0.600345 | 3.4:+61398777 | MS.gene38541:CDS |
| GATCGCAAGAAAGGTCGTGT+TGG | 0.602795 | 3.4:+61398867 | MS.gene38541:CDS |
| CCGGAGACTGAGCTCAAGAG+AGG | 0.605430 | 3.4:+61398618 | MS.gene38541:CDS |
| AAGAAAAGAAGCACCAAGCA+CGG | 0.616563 | 3.4:+61398744 | MS.gene38541:CDS |
| ATTACACATTCAATCACAGT+AGG | 0.621264 | 3.4:+61398513 | MS.gene38541:CDS |
| ACATTCAATCACAGTAGGTG+AGG | 0.621727 | 3.4:+61398518 | MS.gene38541:CDS |
| ATCAAATCTAGTGCCCCACA+AGG | 0.641179 | 3.4:-61398456 | None:intergenic |
| AAGGTCGTGTTGGAATATGG+AGG | 0.705859 | 3.4:+61398877 | MS.gene38541:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTACACATTCAATCACAGT+AGG | + | chr3.4:61398513-61398532 | MS.gene38541:CDS | 30.0% | |
| TTCTTCTCCTTATATTGCAA+GGG | - | chr3.4:61398834-61398853 | None:intergenic | 30.0% | |
| TTTCTTCTCCTTATATTGCA+AGG | - | chr3.4:61398835-61398854 | None:intergenic | 30.0% | |
| ! | TCAGAAATACTTGCTAAATG+AGG | - | chr3.4:61398903-61398922 | None:intergenic | 30.0% |
| !! | TAATAGCTTAAGGTGTTGTT+TGG | + | chr3.4:61398422-61398441 | MS.gene38541:CDS | 30.0% |
| AACATCACCCTTGCAATATA+AGG | + | chr3.4:61398824-61398843 | MS.gene38541:CDS | 35.0% | |
| AAGCATATACTTCTTGATCC+CGG | + | chr3.4:61398641-61398660 | MS.gene38541:CDS | 35.0% | |
| AGCCATTCAAATGCACTATT+CGG | - | chr3.4:61398482-61398501 | None:intergenic | 35.0% | |
| ATCCGAATAGTGCATTTGAA+TGG | + | chr3.4:61398477-61398496 | MS.gene38541:CDS | 35.0% | |
| GATCATGACAATAATGGTAG+TGG | + | chr3.4:61398777-61398796 | MS.gene38541:CDS | 35.0% | |
| GATGGTGATCATGACAATAA+TGG | + | chr3.4:61398771-61398790 | MS.gene38541:CDS | 35.0% | |
| GTTTGCTTAGAACATGGTTA+GGG | - | chr3.4:61398557-61398576 | None:intergenic | 35.0% | |
| !! | AACTTGGTTTGCTTAGAACA+TGG | - | chr3.4:61398563-61398582 | None:intergenic | 35.0% |
| !! | TCGGATTTTGATCATTTCAC+CGG | + | chr3.4:61398599-61398618 | MS.gene38541:CDS | 35.0% |
| ACATTCAATCACAGTAGGTG+AGG | + | chr3.4:61398518-61398537 | MS.gene38541:CDS | 40.0% | |
| AGAAAGGTCGTGTTGGAATA+TGG | + | chr3.4:61398874-61398893 | MS.gene38541:CDS | 40.0% | |
| AGGAGAAGAAATCCTCAAGA+CGG | + | chr3.4:61398844-61398863 | MS.gene38541:CDS | 40.0% | |
| CTAAGCAAACCAAGTTCTCA+AGG | + | chr3.4:61398567-61398586 | MS.gene38541:CDS | 40.0% | |
| ! | GATCCATTCCTTTTGTTCTC+CGG | - | chr3.4:61398678-61398697 | None:intergenic | 40.0% |
| ! | GGTTTGCTTAGAACATGGTT+AGG | - | chr3.4:61398558-61398577 | None:intergenic | 40.0% |
| !! | AAGAAAAGAAGCACCAAGCA+CGG | + | chr3.4:61398744-61398763 | MS.gene38541:CDS | 40.0% |
| !! | GACAATAATGGTAGTGGTGA+TGG | + | chr3.4:61398783-61398802 | MS.gene38541:CDS | 40.0% |
| AAGGTCGTGTTGGAATATGG+AGG | + | chr3.4:61398877-61398896 | MS.gene38541:CDS | 45.0% | |
| ATCAAATCTAGTGCCCCACA+AGG | - | chr3.4:61398459-61398478 | None:intergenic | 45.0% | |
| CATCTTTGCCGGAGAACAAA+AGG | + | chr3.4:61398667-61398686 | MS.gene38541:CDS | 45.0% | |
| GGAGAAGAAATCCTCAAGAC+GGG | + | chr3.4:61398845-61398864 | MS.gene38541:CDS | 45.0% | |
| TTGCCGGAGAACAAAAGGAA+TGG | + | chr3.4:61398672-61398691 | MS.gene38541:CDS | 45.0% | |
| ! | GAACATGGTTAGGGTTTGCT+TGG | - | chr3.4:61398548-61398567 | None:intergenic | 45.0% |
| ! | TGGCTTGTGTTCTTCCTTGT+GGG | + | chr3.4:61398442-61398461 | MS.gene38541:CDS | 45.0% |
| !! | TTGGCTTGTGTTCTTCCTTG+TGG | + | chr3.4:61398441-61398460 | MS.gene38541:CDS | 45.0% |
| ATCACCATCATCACCGTGCT+TGG | - | chr3.4:61398760-61398779 | None:intergenic | 50.0% | |
| CGAACAACGCCTTGAGAACT+TGG | - | chr3.4:61398579-61398598 | None:intergenic | 50.0% | |
| GATCGCAAGAAAGGTCGTGT+TGG | + | chr3.4:61398867-61398886 | MS.gene38541:CDS | 50.0% | |
| GGCTTGTGTTCTTCCTTGTG+GGG | + | chr3.4:61398443-61398462 | MS.gene38541:CDS | 50.0% | |
| TCAAGACGGGATCGCAAGAA+AGG | + | chr3.4:61398858-61398877 | MS.gene38541:CDS | 50.0% | |
| TTCTCCGGCAAAGATGTTGC+CGG | - | chr3.4:61398663-61398682 | None:intergenic | 50.0% | |
| TTTCTTGCGATCCCGTCTTG+AGG | - | chr3.4:61398859-61398878 | None:intergenic | 50.0% | |
| !! | GTTCTCAAGGCGTTGTTCGT+CGG | + | chr3.4:61398580-61398599 | MS.gene38541:CDS | 50.0% |
| AGCACCAAGCACGGTGATGA+TGG | + | chr3.4:61398753-61398772 | MS.gene38541:CDS | 55.0% | |
| CCTCTCTTGAGCTCAGTCTC+CGG | - | chr3.4:61398621-61398640 | None:intergenic | 55.0% | |
| GATCCCGGCAACATCTTTGC+CGG | + | chr3.4:61398656-61398675 | MS.gene38541:CDS | 55.0% | |
| TCTCCGGCAAAGATGTTGCC+GGG | - | chr3.4:61398662-61398681 | None:intergenic | 55.0% | |
| CCGGAGACTGAGCTCAAGAG+AGG | + | chr3.4:61398618-61398637 | MS.gene38541:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 61398417 | 61398929 | 61398417 | ID=MS.gene38541 |
| chr3.4 | mRNA | 61398417 | 61398929 | 61398417 | ID=MS.gene38541.t1;Parent=MS.gene38541 |
| chr3.4 | exon | 61398417 | 61398929 | 61398417 | ID=MS.gene38541.t1.exon1;Parent=MS.gene38541.t1 |
| chr3.4 | CDS | 61398417 | 61398929 | 61398417 | ID=cds.MS.gene38541.t1;Parent=MS.gene38541.t1 |
| Gene Sequence |
| Protein sequence |
