Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39263.t1 | KEH41834.1 | 97.6 | 166 | 4 | 0 | 1 | 166 | 121 | 286 | 1.50E-83 | 318.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39263.t1 | A0A072VII9 | 97.6 | 166 | 4 | 0 | 1 | 166 | 121 | 286 | 1.1e-83 | 318.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055778 | MS.gene39263 | 0.81298 | 3.23E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39263.t1 | MTR_1g055160 | 97.605 | 167 | 4 | 0 | 1 | 167 | 121 | 287 | 1.30e-116 | 333 |
| MS.gene39263.t1 | MTR_1g055160 | 97.605 | 167 | 4 | 0 | 1 | 167 | 123 | 289 | 1.53e-116 | 333 |
| MS.gene39263.t1 | MTR_4g076880 | 91.954 | 174 | 14 | 0 | 1 | 174 | 123 | 296 | 1.72e-113 | 325 |
| MS.gene39263.t1 | MTR_1g055145 | 85.882 | 85 | 12 | 0 | 11 | 95 | 196 | 280 | 1.35e-48 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39263.t1 | AT3G13410 | 44.379 | 169 | 89 | 3 | 1 | 167 | 126 | 291 | 1.63e-42 | 145 |
Find 46 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGAAGACTCATTGGTTTC+AGG | 0.174899 | 1.1:+43110078 | MS.gene39263:CDS |
| CAATCCAGTATCCTTCTTAT+AGG | 0.262025 | 1.1:+43110586 | MS.gene39263:CDS |
| CCAGCTTAAATCATCACTTT+TGG | 0.275529 | 1.1:+43110683 | MS.gene39263:CDS |
| TTTCCTTGGATCTTGCTCTC+TGG | 0.291398 | 1.1:+43110146 | MS.gene39263:CDS |
| TTCCTTCCTCTTGGTCAAAT+AGG | 0.304215 | 1.1:-43110313 | None:intergenic |
| TTCCTTGGATCTTGCTCTCT+GGG | 0.304645 | 1.1:+43110147 | MS.gene39263:CDS |
| ATCAGCTCTGTAGAGGAATC+TGG | 0.330362 | 1.1:+43110522 | MS.gene39263:CDS |
| AATCCAGTATCCTTCTTATA+GGG | 0.339157 | 1.1:+43110587 | MS.gene39263:CDS |
| TGTGGAGATGATTTAGGAAT+TGG | 0.359594 | 1.1:+43110114 | MS.gene39263:CDS |
| GTGGCTCTCTTCCTTCCTCT+TGG | 0.374455 | 1.1:-43110322 | None:intergenic |
| CCACAAAGGGAAAACAGATT+TGG | 0.378624 | 1.1:+43110341 | MS.gene39263:CDS |
| AATCCCTATAAGAAGGATAC+TGG | 0.386646 | 1.1:-43110590 | None:intergenic |
| GAAGCCTGTGGAGATGATTT+AGG | 0.390687 | 1.1:+43110108 | MS.gene39263:CDS |
| TGTGAACTTGGAAGACTCAT+TGG | 0.398899 | 1.1:+43110071 | MS.gene39263:CDS |
| TCCATCACAAGCTGTAGAAT+TGG | 0.403651 | 1.1:-43110655 | None:intergenic |
| ATTGGCAACGTTGCTTTCCT+TGG | 0.404386 | 1.1:+43110132 | MS.gene39263:CDS |
| TCCTCTGATGCTGAAACATA+TGG | 0.415932 | 1.1:-43110048 | None:intergenic |
| AGCATCAGAGGATGTGAACT+TGG | 0.456717 | 1.1:+43110059 | MS.gene39263:CDS |
| GGATCTTGCTCTCTGGGCAC+TGG | 0.458862 | 1.1:+43110153 | MS.gene39263:CDS |
| TTTCTAGCAGAAAGTACAGC+AGG | 0.469614 | 1.1:+43110621 | MS.gene39263:CDS |
| TCAGCTCTGTAGAGGAATCT+GGG | 0.496576 | 1.1:+43110523 | MS.gene39263:CDS |
| ATGTTCACGTATCAGATTGA+AGG | 0.497485 | 1.1:-43111149 | None:intergenic |
| GGAAAGATGCCACGCAATAG+TGG | 0.510171 | 1.1:-43111111 | None:intergenic |
| GGAAGGAAGAGAGCCACAAA+GGG | 0.523325 | 1.1:+43110328 | MS.gene39263:CDS |
| GCAGAAAGTACAGCAGGGAA+TGG | 0.529627 | 1.1:+43110627 | MS.gene39263:CDS |
| TCCATATGTTTCAGCATCAG+AGG | 0.529796 | 1.1:+43110047 | MS.gene39263:CDS |
| CTTTGCAAATCCCTATAAGA+AGG | 0.533063 | 1.1:-43110597 | None:intergenic |
| AGGAAGGAAGAGAGCCACAA+AGG | 0.547575 | 1.1:+43110327 | MS.gene39263:CDS |
| CCAAAAGTGATGATTTAAGC+TGG | 0.555189 | 1.1:-43110683 | None:intergenic |
| ACCAATTCTACAGCTTGTGA+TGG | 0.566623 | 1.1:+43110654 | MS.gene39263:CDS |
| AATTCCTAAATCATCTCCAC+AGG | 0.570593 | 1.1:-43110112 | None:intergenic |
| TGCCCAGAGAGCAAGATCCA+AGG | 0.571107 | 1.1:-43110149 | None:intergenic |
| TGTTCACGTATCAGATTGAA+GGG | 0.576011 | 1.1:-43111148 | None:intergenic |
| TCAGGATTTGCTGAAGCCTG+TGG | 0.581197 | 1.1:+43110096 | MS.gene39263:CDS |
| GATTTGGTTGTGTTCTGCAA+TGG | 0.583901 | 1.1:+43110357 | MS.gene39263:CDS |
| CTTCTTATAGGGATTTGCAA+AGG | 0.595579 | 1.1:+43110598 | MS.gene39263:CDS |
| GGCCTATTTGACCAAGAGGA+AGG | 0.601192 | 1.1:+43110311 | MS.gene39263:intron |
| TACAGGCCTATTTGACCAAG+AGG | 0.610661 | 1.1:+43110307 | MS.gene39263:intron |
| TGAGCTTATCAGCTCTGTAG+AGG | 0.612011 | 1.1:+43110515 | MS.gene39263:CDS |
| TGAAGGGCAACAGTTCTACA+AGG | 0.627055 | 1.1:-43111132 | None:intergenic |
| GTTGACAGAACACAGTCTGA+AGG | 0.645138 | 1.1:+43110399 | MS.gene39263:CDS |
| AACTTCTAGCCACTATTGCG+TGG | 0.648377 | 1.1:+43111102 | MS.gene39263:intron |
| TTCTAGCAGAAAGTACAGCA+GGG | 0.653017 | 1.1:+43110622 | MS.gene39263:CDS |
| TTTATGTGTCAGATCTCTCG+AGG | 0.658047 | 1.1:+43110562 | MS.gene39263:CDS |
| CAGCTCTGTAGAGGAATCTG+GGG | 0.672726 | 1.1:+43110524 | MS.gene39263:CDS |
| TGAAACATATGGAAATGCCA+TGG | 0.679330 | 1.1:-43110037 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATGTTTTATTCATAAGA+AGG | - | chr1.1:43110830-43110849 | None:intergenic | 15.0% |
| !! | ATTAACTGCATTTGTAAAAA+TGG | - | chr1.1:43110737-43110756 | None:intergenic | 20.0% |
| !! | ATTATCTATTGTAACTTTAC+AGG | + | chr1.1:43110290-43110309 | MS.gene39263:intron | 20.0% |
| !! | TTAACTGCATTTGTAAAAAT+GGG | - | chr1.1:43110736-43110755 | None:intergenic | 20.0% |
| !!! | TCATGCATTTGTATATTTTT+AGG | + | chr1.1:43110480-43110499 | MS.gene39263:intron | 20.0% |
| ! | AGATAATTTAATCACAGAGA+AGG | - | chr1.1:43110912-43110931 | None:intergenic | 25.0% |
| ! | AGTTGTATCATTGAAATTTG+AGG | - | chr1.1:43111004-43111023 | None:intergenic | 25.0% |
| ! | ATGATAAAAGAGTGGTATAA+TGG | - | chr1.1:43110860-43110879 | None:intergenic | 25.0% |
| ! | TTACAAATGCAGTTAATTGA+TGG | + | chr1.1:43110740-43110759 | MS.gene39263:intron | 25.0% |
| ! | TTCTCTGTGATTAAATTATC+TGG | + | chr1.1:43110911-43110930 | MS.gene39263:intron | 25.0% |
| !! | AACTATGCAATTGGTTATTT+TGG | + | chr1.1:43111069-43111088 | MS.gene39263:intron | 25.0% |
| !! | ATGACTGATTATGGAATTTT+AGG | + | chr1.1:43110235-43110254 | MS.gene39263:intron | 25.0% |
| AATCCAGTATCCTTCTTATA+GGG | + | chr1.1:43110587-43110606 | MS.gene39263:CDS | 30.0% | |
| AATGGAACATGATAAAAGAG+TGG | - | chr1.1:43110868-43110887 | None:intergenic | 30.0% | |
| AGCAAATGGTAAGATTATGT+CGG | - | chr1.1:43110454-43110473 | None:intergenic | 30.0% | |
| ATTTAATCACAGAGAAGGAA+AGG | - | chr1.1:43110907-43110926 | None:intergenic | 30.0% | |
| CTTCATATGCATGTTTGTAT+AGG | + | chr1.1:43110947-43110966 | MS.gene39263:intron | 30.0% | |
| GGGAAAAACAGAAGAAAAAA+TGG | - | chr1.1:43110886-43110905 | None:intergenic | 30.0% | |
| TACAGAAGCGAATAAAGAAA+AGG | - | chr1.1:43111047-43111066 | None:intergenic | 30.0% | |
| TACATCTACTTCAGAGTTAT+GGG | + | chr1.1:43110422-43110441 | MS.gene39263:intron | 30.0% | |
| TTCTGTACAAACTATGCAAT+TGG | + | chr1.1:43111060-43111079 | MS.gene39263:intron | 30.0% | |
| TTTAATCACAGAGAAGGAAA+GGG | - | chr1.1:43110906-43110925 | None:intergenic | 30.0% | |
| !! | CTTAAATCATCACTTTTGGA+GGG | + | chr1.1:43110687-43110706 | MS.gene39263:CDS | 30.0% |
| !! | TTTTAGGTCCTTCTTTGTAA+TGG | + | chr1.1:43110251-43110270 | MS.gene39263:intron | 30.0% |
| !!! | TCAACATTTTTTGAAGCTTG+AGG | - | chr1.1:43110384-43110403 | None:intergenic | 30.0% |
| AATCCCTATAAGAAGGATAC+TGG | - | chr1.1:43110593-43110612 | None:intergenic | 35.0% | |
| AATTCCTAAATCATCTCCAC+AGG | - | chr1.1:43110115-43110134 | None:intergenic | 35.0% | |
| ATGCATATGAAGTGTACTAG+TGG | - | chr1.1:43110939-43110958 | None:intergenic | 35.0% | |
| CAATCCAGTATCCTTCTTAT+AGG | + | chr1.1:43110586-43110605 | MS.gene39263:CDS | 35.0% | |
| CAGAATAGCACATTCATAGA+AGG | - | chr1.1:43110795-43110814 | None:intergenic | 35.0% | |
| CCAAAAGTGATGATTTAAGC+TGG | - | chr1.1:43110686-43110705 | None:intergenic | 35.0% | |
| CTTCTTATAGGGATTTGCAA+AGG | + | chr1.1:43110598-43110617 | MS.gene39263:CDS | 35.0% | |
| CTTTGCAAATCCCTATAAGA+AGG | - | chr1.1:43110600-43110619 | None:intergenic | 35.0% | |
| GTACATCTACTTCAGAGTTA+TGG | + | chr1.1:43110421-43110440 | MS.gene39263:intron | 35.0% | |
| TCATGCAACCATTACAAAGA+AGG | - | chr1.1:43110262-43110281 | None:intergenic | 35.0% | |
| TCATGTGACATGACTGATTA+TGG | + | chr1.1:43110226-43110245 | MS.gene39263:intron | 35.0% | |
| TGTGGAGATGATTTAGGAAT+TGG | + | chr1.1:43110114-43110133 | MS.gene39263:CDS | 35.0% | |
| ! | CCAGCTTAAATCATCACTTT+TGG | + | chr1.1:43110683-43110702 | MS.gene39263:CDS | 35.0% |
| ! | CTTCTATGAATGTGCTATTC+TGG | + | chr1.1:43110793-43110812 | MS.gene39263:intron | 35.0% |
| !! | GCTTAAATCATCACTTTTGG+AGG | + | chr1.1:43110686-43110705 | MS.gene39263:CDS | 35.0% |
| ACCAATTCTACAGCTTGTGA+TGG | + | chr1.1:43110654-43110673 | MS.gene39263:CDS | 40.0% | |
| ATGCATGAGAACTCAGCAAA+TGG | - | chr1.1:43110468-43110487 | None:intergenic | 40.0% | |
| CCACAAAGGGAAAACAGATT+TGG | + | chr1.1:43110341-43110360 | MS.gene39263:CDS | 40.0% | |
| TCCATATGTTTCAGCATCAG+AGG | + | chr1.1:43110047-43110066 | MS.gene39263:CDS | 40.0% | |
| TCCATCACAAGCTGTAGAAT+TGG | - | chr1.1:43110658-43110677 | None:intergenic | 40.0% | |
| TCCTCTGATGCTGAAACATA+TGG | - | chr1.1:43110051-43110070 | None:intergenic | 40.0% | |
| TGTGAACTTGGAAGACTCAT+TGG | + | chr1.1:43110071-43110090 | MS.gene39263:CDS | 40.0% | |
| TTCCTTCCTCTTGGTCAAAT+AGG | - | chr1.1:43110316-43110335 | None:intergenic | 40.0% | |
| TTTATGTGTCAGATCTCTCG+AGG | + | chr1.1:43110562-43110581 | MS.gene39263:CDS | 40.0% | |
| ! | CCAAATCTGTTTTCCCTTTG+TGG | - | chr1.1:43110344-43110363 | None:intergenic | 40.0% |
| ! | GATTTGGTTGTGTTCTGCAA+TGG | + | chr1.1:43110357-43110376 | MS.gene39263:CDS | 40.0% |
| ! | TTCTAGCAGAAAGTACAGCA+GGG | + | chr1.1:43110622-43110641 | MS.gene39263:CDS | 40.0% |
| ! | TTTCTAGCAGAAAGTACAGC+AGG | + | chr1.1:43110621-43110640 | MS.gene39263:CDS | 40.0% |
| !! | ACTTTTGGAGGGACTTTTAG+TGG | + | chr1.1:43110698-43110717 | MS.gene39263:CDS | 40.0% |
| !! | TTGGAAGACTCATTGGTTTC+AGG | + | chr1.1:43110078-43110097 | MS.gene39263:CDS | 40.0% |
| AACTTCTAGCCACTATTGCG+TGG | + | chr1.1:43111102-43111121 | MS.gene39263:intron | 45.0% | |
| AGCATCAGAGGATGTGAACT+TGG | + | chr1.1:43110059-43110078 | MS.gene39263:CDS | 45.0% | |
| ATCAGCTCTGTAGAGGAATC+TGG | + | chr1.1:43110522-43110541 | MS.gene39263:CDS | 45.0% | |
| GAAGCCTGTGGAGATGATTT+AGG | + | chr1.1:43110108-43110127 | MS.gene39263:CDS | 45.0% | |
| GTTGACAGAACACAGTCTGA+AGG | + | chr1.1:43110399-43110418 | MS.gene39263:CDS | 45.0% | |
| TACAGGCCTATTTGACCAAG+AGG | + | chr1.1:43110307-43110326 | MS.gene39263:intron | 45.0% | |
| TCAGCTCTGTAGAGGAATCT+GGG | + | chr1.1:43110523-43110542 | MS.gene39263:CDS | 45.0% | |
| TGAAGGGCAACAGTTCTACA+AGG | - | chr1.1:43111135-43111154 | None:intergenic | 45.0% | |
| TGAGCTTATCAGCTCTGTAG+AGG | + | chr1.1:43110515-43110534 | MS.gene39263:CDS | 45.0% | |
| TTCCTTGGATCTTGCTCTCT+GGG | + | chr1.1:43110147-43110166 | MS.gene39263:CDS | 45.0% | |
| TTTCCTTGGATCTTGCTCTC+TGG | + | chr1.1:43110146-43110165 | MS.gene39263:CDS | 45.0% | |
| ! | ATTGGCAACGTTGCTTTCCT+TGG | + | chr1.1:43110132-43110151 | MS.gene39263:CDS | 45.0% |
| AGGAAGGAAGAGAGCCACAA+AGG | + | chr1.1:43110327-43110346 | MS.gene39263:CDS | 50.0% | |
| CAGCTCTGTAGAGGAATCTG+GGG | + | chr1.1:43110524-43110543 | MS.gene39263:CDS | 50.0% | |
| GGAAAGATGCCACGCAATAG+TGG | - | chr1.1:43111114-43111133 | None:intergenic | 50.0% | |
| GGAAGGAAGAGAGCCACAAA+GGG | + | chr1.1:43110328-43110347 | MS.gene39263:CDS | 50.0% | |
| GGCCTATTTGACCAAGAGGA+AGG | + | chr1.1:43110311-43110330 | MS.gene39263:intron | 50.0% | |
| ! | GCAGAAAGTACAGCAGGGAA+TGG | + | chr1.1:43110627-43110646 | MS.gene39263:CDS | 50.0% |
| !! | TCAGGATTTGCTGAAGCCTG+TGG | + | chr1.1:43110096-43110115 | MS.gene39263:CDS | 50.0% |
| GTGGCTCTCTTCCTTCCTCT+TGG | - | chr1.1:43110325-43110344 | None:intergenic | 55.0% | |
| TGCCCAGAGAGCAAGATCCA+AGG | - | chr1.1:43110152-43110171 | None:intergenic | 55.0% | |
| GGATCTTGCTCTCTGGGCAC+TGG | + | chr1.1:43110153-43110172 | MS.gene39263:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 43110039 | 43111167 | 43110039 | ID=MS.gene39263 |
| chr1.1 | mRNA | 43110039 | 43111167 | 43110039 | ID=MS.gene39263.t1;Parent=MS.gene39263 |
| chr1.1 | exon | 43110039 | 43110212 | 43110039 | ID=MS.gene39263.t1.exon1;Parent=MS.gene39263.t1 |
| chr1.1 | CDS | 43110039 | 43110212 | 43110039 | ID=cds.MS.gene39263.t1;Parent=MS.gene39263.t1 |
| chr1.1 | exon | 43110312 | 43110420 | 43110312 | ID=MS.gene39263.t1.exon2;Parent=MS.gene39263.t1 |
| chr1.1 | CDS | 43110312 | 43110420 | 43110312 | ID=cds.MS.gene39263.t1;Parent=MS.gene39263.t1 |
| chr1.1 | exon | 43110502 | 43110719 | 43110502 | ID=MS.gene39263.t1.exon3;Parent=MS.gene39263.t1 |
| chr1.1 | CDS | 43110502 | 43110719 | 43110502 | ID=cds.MS.gene39263.t1;Parent=MS.gene39263.t1 |
| chr1.1 | exon | 43111111 | 43111167 | 43111111 | ID=MS.gene39263.t1.exon4;Parent=MS.gene39263.t1 |
| chr1.1 | CDS | 43111111 | 43111167 | 43111111 | ID=cds.MS.gene39263.t1;Parent=MS.gene39263.t1 |
| Gene Sequence |
| Protein sequence |
