Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39269.t1 | RHN38921.1 | 98.7 | 303 | 4 | 0 | 1 | 303 | 1400 | 1702 | 2.10E-167 | 598.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39269.t1 | G7LDJ1 | 98.7 | 303 | 4 | 0 | 1 | 303 | 1397 | 1699 | 1.5e-167 | 598.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053900 | MS.gene39269 | 0.811097 | 8.30E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39269.t1 | MTR_8g011210 | 98.680 | 303 | 4 | 0 | 1 | 303 | 1397 | 1699 | 0.0 | 612 |
| MS.gene39269.t1 | MTR_8g011330 | 85.479 | 303 | 44 | 0 | 1 | 303 | 1400 | 1702 | 2.20e-177 | 533 |
| MS.gene39269.t1 | MTR_8g011230 | 83.721 | 301 | 49 | 0 | 1 | 301 | 1396 | 1696 | 4.69e-173 | 521 |
| MS.gene39269.t1 | MTR_3g408440 | 80.731 | 301 | 56 | 1 | 1 | 301 | 1409 | 1707 | 5.90e-163 | 495 |
| MS.gene39269.t1 | MTR_8g090165 | 69.408 | 304 | 92 | 1 | 1 | 303 | 1399 | 1702 | 3.81e-135 | 419 |
| MS.gene39269.t1 | MTR_2g067030 | 68.543 | 302 | 94 | 1 | 1 | 301 | 1283 | 1584 | 4.25e-132 | 410 |
| MS.gene39269.t1 | MTR_8g011200 | 65.132 | 304 | 96 | 3 | 1 | 303 | 1343 | 1637 | 3.51e-122 | 383 |
| MS.gene39269.t1 | MTR_6g015173 | 60.927 | 302 | 60 | 3 | 1 | 302 | 1074 | 1317 | 1.50e-109 | 345 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39269.t1 | AT3G48770 | 38.188 | 309 | 178 | 6 | 3 | 300 | 1589 | 1895 | 7.37e-58 | 201 |
| MS.gene39269.t1 | AT3G48770 | 38.188 | 309 | 178 | 6 | 3 | 300 | 1589 | 1895 | 7.37e-58 | 201 |
Find 48 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTGTGCTTTCCCGAAAA+TGG | 0.156225 | 8.2:+80689008 | MS.gene39269:CDS |
| TTTAAGTTGAAAATGTTATT+TGG | 0.172271 | 8.2:-80688955 | None:intergenic |
| GGGAAATAATGAAATATTTC+TGG | 0.228636 | 8.2:+80688711 | MS.gene39269:CDS |
| ACAGCTTCATGTCTCTTCTT+TGG | 0.236383 | 8.2:-80689033 | None:intergenic |
| CAGGAACTTCCTCCATCTTC+TGG | 0.281536 | 8.2:-80689200 | None:intergenic |
| CTTCTTCATATGAAGGTTAT+CGG | 0.330981 | 8.2:-80688758 | None:intergenic |
| ATGATGAGTGCTGCAAGTTC+TGG | 0.350900 | 8.2:+80688611 | MS.gene39269:CDS |
| GGGTTGGTTAAGCTTATTCT+TGG | 0.377675 | 8.2:+80688979 | MS.gene39269:CDS |
| TATAGTTTGTCATTGTCATC+AGG | 0.384678 | 8.2:+80689111 | MS.gene39269:CDS |
| CAGAAGATGGAGGAAGTTCC+TGG | 0.404182 | 8.2:+80689201 | MS.gene39269:CDS |
| AGCAAACAAAAGGGTGCGTT+GGG | 0.408401 | 8.2:+80689152 | MS.gene39269:CDS |
| TTACAAAGAAAGCAAACAAA+AGG | 0.429034 | 8.2:+80689142 | MS.gene39269:CDS |
| ATGTTGACCTCTGGAATGAT+TGG | 0.446773 | 8.2:+80688563 | MS.gene39269:CDS |
| TTCTTCATATGAAGGTTATC+GGG | 0.463714 | 8.2:-80688757 | None:intergenic |
| TGTTGACCTCTGGAATGATT+GGG | 0.465606 | 8.2:+80688564 | MS.gene39269:CDS |
| AAAATACAGACTTAGTCAAA+AGG | 0.466350 | 8.2:-80689429 | None:intergenic |
| CTCTCCGAACTAATCACATT+AGG | 0.476446 | 8.2:+80689306 | MS.gene39269:CDS |
| TCAGATTGACCTTGAGGATG+AGG | 0.477820 | 8.2:+80689386 | MS.gene39269:CDS |
| TCATCGGCTCTGTTGTCTCA+TGG | 0.491753 | 8.2:-80689080 | None:intergenic |
| ATGAAACTACCTACATCATC+GGG | 0.496847 | 8.2:+80688691 | MS.gene39269:CDS |
| TAGAAGATTATGTTGACCTC+TGG | 0.497697 | 8.2:+80688554 | MS.gene39269:CDS |
| AAGCAAACAAAAGGGTGCGT+TGG | 0.498311 | 8.2:+80689151 | MS.gene39269:CDS |
| CAGTTTACCACCGTCATTCA+CGG | 0.499696 | 8.2:-80688920 | None:intergenic |
| AAAGCCTAATGTGATTAGTT+CGG | 0.503520 | 8.2:-80689310 | None:intergenic |
| TCATTATTTCCCGATGATGT+AGG | 0.504523 | 8.2:-80688700 | None:intergenic |
| AAACTATAGCTTACATTCAT+CGG | 0.504982 | 8.2:-80689096 | None:intergenic |
| CAAGTTTATCATCCAGAAGA+TGG | 0.507179 | 8.2:+80689188 | MS.gene39269:CDS |
| GTCAACATAATCTTCTACAT+AGG | 0.509378 | 8.2:-80688548 | None:intergenic |
| AATGTCAGACAGCTCAGACT+TGG | 0.523649 | 8.2:-80688839 | None:intergenic |
| GATGAAACTACCTACATCAT+CGG | 0.535329 | 8.2:+80688690 | MS.gene39269:CDS |
| TACAAAGAAAGCAAACAAAA+GGG | 0.549134 | 8.2:+80689143 | MS.gene39269:CDS |
| TCAACATAATCTTCTACATA+GGG | 0.552184 | 8.2:-80688547 | None:intergenic |
| AAATACAGACTTAGTCAAAA+GGG | 0.556820 | 8.2:-80689428 | None:intergenic |
| GAATCTTCAGATTGACCTTG+AGG | 0.568766 | 8.2:+80689380 | MS.gene39269:CDS |
| TTCTCTGAAGCAATATCTGA+GGG | 0.579971 | 8.2:+80689249 | MS.gene39269:CDS |
| ATAGTTTGTCATTGTCATCA+GGG | 0.588088 | 8.2:+80689112 | MS.gene39269:CDS |
| GCACATCTCAATGCATCACC+AGG | 0.604874 | 8.2:-80689219 | None:intergenic |
| GTTGACCTCTGGAATGATTG+GGG | 0.607615 | 8.2:+80688565 | MS.gene39269:CDS |
| TTTCTCTGAAGCAATATCTG+AGG | 0.611473 | 8.2:+80689248 | MS.gene39269:CDS |
| GAATCATCCTCCGTGAATGA+CGG | 0.618888 | 8.2:+80688910 | MS.gene39269:CDS |
| TAGTTTGTCATTGTCATCAG+GGG | 0.634585 | 8.2:+80689113 | MS.gene39269:CDS |
| ACAGACTTAGTCAAAAGGGA+AGG | 0.635783 | 8.2:-80689424 | None:intergenic |
| TGAGGAACTCCTCATCCTCA+AGG | 0.652480 | 8.2:-80689395 | None:intergenic |
| TCATCCTCCGTGAATGACGG+TGG | 0.674049 | 8.2:+80688913 | MS.gene39269:CDS |
| GTTTATCATCCAGAAGATGG+AGG | 0.678720 | 8.2:+80689191 | MS.gene39269:CDS |
| CAAAAGGGAAGGCAGAACTG+AGG | 0.698592 | 8.2:-80689413 | None:intergenic |
| TTGACCTCTGGAATGATTGG+GGG | 0.722661 | 8.2:+80688566 | MS.gene39269:CDS |
| TTTACCACCGTCATTCACGG+AGG | 0.732229 | 8.2:-80688917 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAACATTTTCAACTTAA+AGG | + | chr8.2:80688957-80688976 | MS.gene39269:CDS | 15.0% |
| !!! | AATAACATTTTCAACTTAAA+GGG | + | chr8.2:80688958-80688977 | MS.gene39269:CDS | 15.0% |
| !!! | TTTAAGTTGAAAATGTTATT+TGG | - | chr8.2:80688958-80688977 | None:intergenic | 15.0% |
| !!! | ATAACATTTTCAACTTAAAG+GGG | + | chr8.2:80688959-80688978 | MS.gene39269:CDS | 20.0% |
| ! | AAACTATAGCTTACATTCAT+CGG | - | chr8.2:80689099-80689118 | None:intergenic | 25.0% |
| ! | GGGAAATAATGAAATATTTC+TGG | + | chr8.2:80688711-80688730 | MS.gene39269:CDS | 25.0% |
| ! | TACAAAGAAAGCAAACAAAA+GGG | + | chr8.2:80689143-80689162 | MS.gene39269:CDS | 25.0% |
| ! | TCAACATAATCTTCTACATA+GGG | - | chr8.2:80688550-80688569 | None:intergenic | 25.0% |
| ! | TCTGACATTTACAGAAAAAT+TGG | + | chr8.2:80688853-80688872 | MS.gene39269:CDS | 25.0% |
| ! | TTACAAAGAAAGCAAACAAA+AGG | + | chr8.2:80689142-80689161 | MS.gene39269:CDS | 25.0% |
| !!! | TGAAGAAATCGATTTTCTAA+TGG | + | chr8.2:80689350-80689369 | MS.gene39269:CDS | 25.0% |
| CAAAAAGCTTCTTCATATGA+AGG | - | chr8.2:80688768-80688787 | None:intergenic | 30.0% | |
| CTTCTTCATATGAAGGTTAT+CGG | - | chr8.2:80688761-80688780 | None:intergenic | 30.0% | |
| GTCAACATAATCTTCTACAT+AGG | - | chr8.2:80688551-80688570 | None:intergenic | 30.0% | |
| TTCTTCATATGAAGGTTATC+GGG | - | chr8.2:80688760-80688779 | None:intergenic | 30.0% | |
| ! | AAAAAGTTTTCATGCTATGC+TGG | - | chr8.2:80688819-80688838 | None:intergenic | 30.0% |
| ! | AAAAGTTTTCATGCTATGCT+GGG | - | chr8.2:80688818-80688837 | None:intergenic | 30.0% |
| ! | AAAGCCTAATGTGATTAGTT+CGG | - | chr8.2:80689313-80689332 | None:intergenic | 30.0% |
| ! | ATAGTTTGTCATTGTCATCA+GGG | + | chr8.2:80689112-80689131 | MS.gene39269:CDS | 30.0% |
| ! | TATAGTTTGTCATTGTCATC+AGG | + | chr8.2:80689111-80689130 | MS.gene39269:CDS | 30.0% |
| !! | ATATGAAGAAGCTTTTTGAG+AGG | + | chr8.2:80688770-80688789 | MS.gene39269:CDS | 30.0% |
| !! | TATGAAGAAGCTTTTTGAGA+GGG | + | chr8.2:80688771-80688790 | MS.gene39269:CDS | 30.0% |
| ATGAAACTACCTACATCATC+GGG | + | chr8.2:80688691-80688710 | MS.gene39269:CDS | 35.0% | |
| CAAGTTTATCATCCAGAAGA+TGG | + | chr8.2:80689188-80689207 | MS.gene39269:CDS | 35.0% | |
| GATGAAACTACCTACATCAT+CGG | + | chr8.2:80688690-80688709 | MS.gene39269:CDS | 35.0% | |
| TAGAAGATTATGTTGACCTC+TGG | + | chr8.2:80688554-80688573 | MS.gene39269:CDS | 35.0% | |
| TCATTATTTCCCGATGATGT+AGG | - | chr8.2:80688703-80688722 | None:intergenic | 35.0% | |
| ! | AAAGTTTTCATGCTATGCTG+GGG | - | chr8.2:80688817-80688836 | None:intergenic | 35.0% |
| ! | CATTTTCAACTTAAAGGGGT+TGG | + | chr8.2:80688963-80688982 | MS.gene39269:CDS | 35.0% |
| ! | TAGTTTGTCATTGTCATCAG+GGG | + | chr8.2:80689113-80689132 | MS.gene39269:CDS | 35.0% |
| !! | CTCTTCTTTGGTTCCATTTT+CGG | - | chr8.2:80689024-80689043 | None:intergenic | 35.0% |
| !! | TCTTCTTTGGTTCCATTTTC+GGG | - | chr8.2:80689023-80689042 | None:intergenic | 35.0% |
| !! | TTCTCTGAAGCAATATCTGA+GGG | + | chr8.2:80689249-80689268 | MS.gene39269:CDS | 35.0% |
| !! | TTTCTCTGAAGCAATATCTG+AGG | + | chr8.2:80689248-80689267 | MS.gene39269:CDS | 35.0% |
| ACAGCTTCATGTCTCTTCTT+TGG | - | chr8.2:80689036-80689055 | None:intergenic | 40.0% | |
| CTCTCCGAACTAATCACATT+AGG | + | chr8.2:80689306-80689325 | MS.gene39269:CDS | 40.0% | |
| GAATCTTCAGATTGACCTTG+AGG | + | chr8.2:80689380-80689399 | MS.gene39269:CDS | 40.0% | |
| GTTTATCATCCAGAAGATGG+AGG | + | chr8.2:80689191-80689210 | MS.gene39269:CDS | 40.0% | |
| ! | AGAGGGAGAAAGTTTTTGTG+TGG | + | chr8.2:80688788-80688807 | MS.gene39269:CDS | 40.0% |
| !! | ATGTTGACCTCTGGAATGAT+TGG | + | chr8.2:80688563-80688582 | MS.gene39269:CDS | 40.0% |
| !! | GGGTTGGTTAAGCTTATTCT+TGG | + | chr8.2:80688979-80688998 | MS.gene39269:CDS | 40.0% |
| !! | TGTTGACCTCTGGAATGATT+GGG | + | chr8.2:80688564-80688583 | MS.gene39269:CDS | 40.0% |
| AATGTCAGACAGCTCAGACT+TGG | - | chr8.2:80688842-80688861 | None:intergenic | 45.0% | |
| CAGTTTACCACCGTCATTCA+CGG | - | chr8.2:80688923-80688942 | None:intergenic | 45.0% | |
| GAATCATCCTCCGTGAATGA+CGG | + | chr8.2:80688910-80688929 | MS.gene39269:CDS | 45.0% | |
| GAATGATTGGGGGAGTTCAA+TGG | + | chr8.2:80688576-80688595 | MS.gene39269:CDS | 45.0% | |
| TCAGATTGACCTTGAGGATG+AGG | + | chr8.2:80689386-80689405 | MS.gene39269:CDS | 45.0% | |
| TGCTTGTGCTTTCCCGAAAA+TGG | + | chr8.2:80689008-80689027 | MS.gene39269:CDS | 45.0% | |
| ! | AAGCAAACAAAAGGGTGCGT+TGG | + | chr8.2:80689151-80689170 | MS.gene39269:CDS | 45.0% |
| ! | AGCAAACAAAAGGGTGCGTT+GGG | + | chr8.2:80689152-80689171 | MS.gene39269:CDS | 45.0% |
| ! | ATGATGAGTGCTGCAAGTTC+TGG | + | chr8.2:80688611-80688630 | MS.gene39269:CDS | 45.0% |
| !! | GTTGACCTCTGGAATGATTG+GGG | + | chr8.2:80688565-80688584 | MS.gene39269:CDS | 45.0% |
| !! | TTGACCTCTGGAATGATTGG+GGG | + | chr8.2:80688566-80688585 | MS.gene39269:CDS | 45.0% |
| AACTCCCCCAATCATTCCAG+AGG | - | chr8.2:80688573-80688592 | None:intergenic | 50.0% | |
| CAAAAGGGAAGGCAGAACTG+AGG | - | chr8.2:80689416-80689435 | None:intergenic | 50.0% | |
| CAGAAGATGGAGGAAGTTCC+TGG | + | chr8.2:80689201-80689220 | MS.gene39269:CDS | 50.0% | |
| CAGGAACTTCCTCCATCTTC+TGG | - | chr8.2:80689203-80689222 | None:intergenic | 50.0% | |
| GCACATCTCAATGCATCACC+AGG | - | chr8.2:80689222-80689241 | None:intergenic | 50.0% | |
| TGAGGAACTCCTCATCCTCA+AGG | - | chr8.2:80689398-80689417 | None:intergenic | 50.0% | |
| TTTACCACCGTCATTCACGG+AGG | - | chr8.2:80688920-80688939 | None:intergenic | 50.0% | |
| !! | TCATCGGCTCTGTTGTCTCA+TGG | - | chr8.2:80689083-80689102 | None:intergenic | 50.0% |
| TCATCCTCCGTGAATGACGG+TGG | + | chr8.2:80688913-80688932 | MS.gene39269:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 80688529 | 80689440 | 80688529 | ID=MS.gene39269 |
| chr8.2 | mRNA | 80688529 | 80689440 | 80688529 | ID=MS.gene39269.t1;Parent=MS.gene39269 |
| chr8.2 | exon | 80688529 | 80689440 | 80688529 | ID=MS.gene39269.t1.exon1;Parent=MS.gene39269.t1 |
| chr8.2 | CDS | 80688529 | 80689440 | 80688529 | ID=cds.MS.gene39269.t1;Parent=MS.gene39269.t1 |
| Gene Sequence |
| Protein sequence |
