AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39491

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39491.t1 MTR_4g088525 91.690 361 30 0 26 386 8 368 0.0 677
MS.gene39491.t1 MTR_8g027395 73.958 384 100 0 3 386 13 396 0.0 580
MS.gene39491.t1 MTR_8g027420 73.438 384 91 1 3 386 2 374 0.0 561
MS.gene39491.t1 MTR_4g088535 74.869 382 80 2 1 382 1 366 0.0 545
MS.gene39491.t1 MTR_3g101010 69.010 384 115 1 7 386 5 388 0.0 512
MS.gene39491.t1 MTR_3g018740 68.848 382 116 2 7 385 5 386 0.0 509
MS.gene39491.t1 MTR_4g045707 67.016 382 124 2 7 386 6 387 3.19e-178 501
MS.gene39491.t1 MTR_3g101030 64.736 397 116 6 12 386 34 428 3.55e-170 482
MS.gene39491.t1 MTR_3g101180 63.706 394 129 4 7 386 6 399 1.33e-166 472
MS.gene39491.t1 MTR_3g048000 62.274 387 125 4 4 386 22 391 4.73e-164 465
MS.gene39491.t1 MTR_8g461160 62.532 387 137 2 7 385 6 392 1.58e-163 464
MS.gene39491.t1 MTR_3g101130 61.954 389 137 4 5 386 4 388 2.75e-162 461
MS.gene39491.t1 MTR_2g045980 63.171 391 133 5 1 385 1 386 5.80e-162 460
MS.gene39491.t1 MTR_2g046030 62.660 391 135 5 1 385 1 386 6.08e-161 457
MS.gene39491.t1 MTR_7g050810 60.465 387 145 2 7 385 16 402 2.41e-160 456
MS.gene39491.t1 MTR_7g451890 58.915 387 151 2 7 385 14 400 2.96e-159 453
MS.gene39491.t1 MTR_7g047780 58.398 387 153 2 7 385 14 400 4.17e-157 448
MS.gene39491.t1 MTR_7g050750 58.398 387 153 2 7 385 8 394 2.47e-156 446
MS.gene39491.t1 MTR_3g015620 58.398 387 129 5 7 385 16 378 8.98e-147 421
MS.gene39491.t1 MTR_3g101050 60.052 383 128 6 11 386 33 397 1.61e-146 421
MS.gene39491.t1 MTR_3g102920 56.955 381 120 6 14 386 65 409 1.22e-132 386
MS.gene39491.t1 MTR_3g101200 54.145 386 144 5 7 385 31 390 2.22e-125 367
MS.gene39491.t1 MTR_3g101190 53.889 360 103 3 29 386 13 311 2.22e-119 348
MS.gene39491.t1 MTR_3g100995 64.567 254 85 2 135 385 11 262 1.21e-113 332
MS.gene39491.t1 MTR_3g100010 55.449 312 91 3 30 308 40 336 9.28e-109 323
MS.gene39491.t1 MTR_3g018747 64.865 259 73 3 131 385 9 253 1.71e-106 314
MS.gene39491.t1 MTR_7g050830 53.896 308 109 3 13 298 22 318 1.66e-103 309
MS.gene39491.t1 MTR_3g015760 57.401 277 106 4 116 385 8 279 2.74e-98 294
MS.gene39491.t1 MTR_3g114310 41.495 388 208 10 12 384 24 407 3.55e-86 268
MS.gene39491.t1 MTR_3g100520 63.426 216 68 4 172 385 1 207 1.39e-84 256
MS.gene39491.t1 MTR_3g111160 67.488 203 63 2 187 386 17 219 5.05e-84 255
MS.gene39491.t1 MTR_3g101110 56.485 239 70 3 131 361 68 280 6.80e-81 249
MS.gene39491.t1 MTR_2g046410 61.574 216 74 3 176 385 135 347 4.11e-78 244
MS.gene39491.t1 MTR_3g099970 61.735 196 58 2 131 326 20 198 1.27e-75 233
MS.gene39491.t1 MTR_3g102870 67.702 161 52 0 194 354 1 161 1.26e-68 214
MS.gene39491.t1 MTR_3g101150 64.968 157 47 1 237 385 1 157 2.03e-62 197
MS.gene39491.t1 MTR_3g101020 54.891 184 54 2 205 385 1 158 1.90e-60 192
MS.gene39491.t1 MTR_3g111150 57.558 172 49 2 11 182 3 150 4.82e-58 187
MS.gene39491.t1 MTR_3g100990 68.504 127 40 0 5 131 3 129 2.55e-50 176
MS.gene39491.t1 MTR_4g093550 66.667 84 28 0 7 90 6 89 5.03e-30 111
MS.gene39491.t1 MTR_6g046890 58.140 86 31 1 15 95 7 92 3.55e-21 89.0
MS.gene39491.t1 MTR_3g101140 55.224 67 25 2 324 385 48 114 1.52e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39491.t1 AT1G47710 50.777 386 183 4 7 385 5 390 4.94e-138 399
MS.gene39491.t1 AT1G47710 50.777 386 183 4 7 385 32 417 4.58e-137 397
MS.gene39491.t1 AT2G25240 49.211 380 181 7 11 384 9 382 4.70e-121 356
MS.gene39491.t1 AT3G45220 46.354 384 194 4 11 384 9 390 1.09e-119 352
MS.gene39491.t1 AT2G26390 45.596 386 190 8 11 384 9 386 4.10e-110 328
MS.gene39491.t1 AT1G62170 43.251 363 175 4 31 384 91 431 8.93e-97 295
MS.gene39491.t1 AT2G14540 43.928 387 186 6 7 384 33 397 1.15e-96 294
MS.gene39491.t1 AT1G64030 39.687 383 210 5 7 384 5 371 1.36e-91 280
MS.gene39491.t1 AT1G62170 39.646 396 175 5 31 384 91 464 1.14e-89 278
MS.gene39491.t1 AT2G35580 40.669 359 184 7 35 384 33 371 2.54e-82 256
MS.gene39491.t1 AT1G64010 44.444 216 96 4 172 384 1 195 2.23e-56 184
MS.gene39491.t1 AT1G64010 43.564 202 90 4 186 384 1 181 9.05e-50 166
MS.gene39491.t1 AT1G63280 52.174 115 51 2 131 243 8 120 2.53e-31 115
MS.gene39491.t1 AT1G62160 39.205 176 86 4 211 384 62 218 5.46e-31 118
MS.gene39491.t1 AT1G51330 52.632 95 45 0 130 224 30 124 2.55e-25 102
MS.gene39491.t1 AT1G64020 33.929 112 71 3 12 121 10 120 6.79e-12 62.8

Find 63 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAAAAGAAGTGAATTTA+TGG 0.203774 4.2:-22617771 MS.gene39491:CDS
CTAGATGAAAATTTGAATCT+TGG 0.311615 4.2:+22617340 None:intergenic
CTACTTCTCATACCTTGTTC+TGG 0.331685 4.2:+22618482 None:intergenic
ACCCAAACAATCCTCTTTGC+CGG 0.332257 4.2:-22617061 MS.gene39491:CDS
GTCCGGCAAAGAGGATTGTT+TGG 0.343638 4.2:+22617059 None:intergenic
AATAAATTGATTAAAGTTGC+TGG 0.347376 4.2:+22617558 None:intergenic
TGAAGGAAGGTTGAGGAGAA+AGG 0.364807 4.2:+22618554 None:intergenic
TCCGGCAAAGAGGATTGTTT+GGG 0.390129 4.2:+22617060 None:intergenic
CGATGATTGCAAGCACTACT+TGG 0.408491 4.2:+22618749 None:intergenic
TATAAGGAAGCTTGTGCTCT+AGG 0.415442 4.2:+22617392 None:intergenic
GATGATCGGTGGATTTGAAT+TGG 0.415633 4.2:+22618680 None:intergenic
TTTCTTCCAAATGCAAAAGA+TGG 0.417147 4.2:-22617457 MS.gene39491:CDS
GAAGGAAGGTTGAGGAGAAA+GGG 0.432354 4.2:+22618555 None:intergenic
TTTACCATGGGTAGATCAAC+CGG 0.448059 4.2:-22617136 MS.gene39491:CDS
GACAAAAGAAGTGAATTTAT+GGG 0.449535 4.2:-22617770 MS.gene39491:CDS
AGACAAAGTGGCCTTGTAAT+CGG 0.455993 4.2:+22618516 None:intergenic
GTCAGCTTTGGTTGAGAAGG+TGG 0.459796 4.2:-22617431 MS.gene39491:CDS
ATCATCGCTGCTGGCTCTGA+CGG 0.466572 4.2:-22618733 MS.gene39491:CDS
TTTATTGAAGTAAACGAGAA+AGG 0.470403 4.2:-22617190 MS.gene39491:CDS
ATTAAAGTTGCTGGTCATGA+AGG 0.473995 4.2:+22617567 None:intergenic
AAGAGGGTTTAGCACTTGTC+CGG 0.477706 4.2:+22617042 None:intergenic
AAAATGGTGGACTCTCTTGA+AGG 0.485242 4.2:-22617250 MS.gene39491:CDS
AAAAGATGGTCTGTCAGCTT+TGG 0.500468 4.2:-22617443 MS.gene39491:CDS
CATGGTAAAATATGCAGTAC+TGG 0.501847 4.2:+22617150 None:intergenic
CTTCGTCTTCCTTATAAGCA+AGG 0.506335 4.2:-22617511 MS.gene39491:CDS
TTACTGAGCGTCCATTAAGA+AGG 0.506567 4.2:+22617596 None:intergenic
ATCAGCTATAAAGTCTAGTC+CGG 0.515418 4.2:+22617117 None:intergenic
GTGCTTGCAATCATCGCTGC+TGG 0.516685 4.2:-22618742 MS.gene39491:CDS
AGAAAGGTAAAATCACTCCT+AGG 0.522055 4.2:+22617290 None:intergenic
GTTAAGATCACTCAGCAAAG+AGG 0.525451 4.2:+22617025 None:intergenic
TTCTGTTGATTTCCAGAACA+AGG 0.528215 4.2:-22618494 MS.gene39491:intron
TCTTAATGGACGCTCAGTAA+AGG 0.535528 4.2:-22617593 MS.gene39491:CDS
TATCGTGTCTACCGATTACA+AGG 0.540227 4.2:-22618527 MS.gene39491:CDS
GATCGGTGGATTTGAATTGG+AGG 0.547118 4.2:+22618683 None:intergenic
TGCGTTAGCAAAGATGAGTC+GGG 0.554115 4.2:+22617684 None:intergenic
TATAAAACAGTGCGAGTAGG+AGG 0.555196 4.2:-22617373 MS.gene39491:CDS
GGTAGACACGATATGTTTGA+AGG 0.558443 4.2:+22618537 None:intergenic
TTAAGATCACTCAGCAAAGA+GGG 0.559364 4.2:+22617026 None:intergenic
GACGAAGGAGTTGAGATGAT+CGG 0.564397 4.2:+22618666 None:intergenic
GTGCGTTAGCAAAGATGAGT+CGG 0.566863 4.2:+22617683 None:intergenic
ATATGTTTGAAGGAAGGTTG+AGG 0.570373 4.2:+22618547 None:intergenic
GACACGATATGTTTGAAGGA+AGG 0.570621 4.2:+22618541 None:intergenic
TGAGAAAAGATGTTTGGCTA+TGG 0.572303 4.2:+22618813 None:intergenic
AGTAGCATCTTTGAAGATGA+CGG 0.575385 4.2:+22618630 None:intergenic
TCTGTCAGCTTTGGTTGAGA+AGG 0.577265 4.2:-22617434 MS.gene39491:CDS
AAGATGTTTGGCTATGGTGA+GGG 0.583307 4.2:+22618819 None:intergenic
TTAAAGTTGCTGGTCATGAA+GGG 0.586835 4.2:+22617568 None:intergenic
CTTCTGAAATATGTTGCTAA+CGG 0.590462 4.2:+22617216 None:intergenic
TGGTGAGGGAAACTTTCGTA+TGG 0.600494 4.2:+22618833 None:intergenic
GAAAGGCACTAAAGCTGTTG+CGG 0.603147 4.2:-22617173 MS.gene39491:CDS
AAAGATGTTTGGCTATGGTG+AGG 0.605145 4.2:+22618818 None:intergenic
GTCGAGAAGCTGTTGTTGTG+TGG 0.618987 4.2:+22618708 None:intergenic
TTAGCACTTGTCCGGCAAAG+AGG 0.620381 4.2:+22617050 None:intergenic
CCTTATAAAACAGTGCGAGT+AGG 0.620740 4.2:-22617376 MS.gene39491:CDS
GAAATCAACAGAAGACAAAG+TGG 0.639195 4.2:+22618504 None:intergenic
GGAAATGAGTTGAGAGACGA+AGG 0.639293 4.2:+22618651 None:intergenic
AAGAGGCGAGGACCACGACA+AGG 0.644610 4.2:+22618607 None:intergenic
AAAGATGCTACTCCTTGTCG+TGG 0.648296 4.2:-22618619 MS.gene39491:CDS
GAAGGAGTTGAGATGATCGG+TGG 0.649562 4.2:+22618669 None:intergenic
TCTCATATACTGAAAGACCT+AGG 0.652170 4.2:-22617307 MS.gene39491:CDS
AGCACTACTTGGAGAGACAA+AGG 0.653123 4.2:+22618760 None:intergenic
TAGTCCGGTTGATCTACCCA+TGG 0.686736 4.2:+22617132 None:intergenic
ATAAGCAAGGAAAAGATGAG+CGG 0.694027 4.2:-22617498 MS.gene39491:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTTGTCATATATTT+AGG + chr4.2:22618276-22618295 None:intergenic 10.0%
!! CTCTAATTAAAAATAAGAAA+GGG + chr4.2:22618812-22618831 None:intergenic 15.0%
!! TCTCTAATTAAAAATAAGAA+AGG + chr4.2:22618813-22618832 None:intergenic 15.0%
!! AATAAATTGATTAAAGTTGC+TGG + chr4.2:22618346-22618365 None:intergenic 20.0%
!! ATATGTAAATTTGATTGACA+TGG - chr4.2:22618089-22618108 MS.gene39491:intron 20.0%
!! ATTAAAAAAATTGTTGCTCA+AGG - chr4.2:22618177-22618196 MS.gene39491:intron 20.0%
!! TTAAAAAAATTGTTGCTCAA+GGG - chr4.2:22618178-22618197 MS.gene39491:intron 20.0%
!!! CAATTTATTAGAGTTTTTGA+TGG - chr4.2:22618357-22618376 MS.gene39491:intron 20.0%
!!! GAGCAACAATTTTTTTAATA+AGG + chr4.2:22618176-22618195 None:intergenic 20.0%
! CTAGATGAAAATTTGAATCT+TGG + chr4.2:22618564-22618583 None:intergenic 25.0%
! CTTCATGAATAACATTCAAA+AGG - chr4.2:22617712-22617731 MS.gene39491:CDS 25.0%
! GACAAAAGAAGTGAATTTAT+GGG - chr4.2:22618131-22618150 MS.gene39491:intron 25.0%
! TATAAACGGTGTTTGATTAA+TGG - chr4.2:22617593-22617612 MS.gene39491:CDS 25.0%
! TGACAAAAGAAGTGAATTTA+TGG - chr4.2:22618130-22618149 MS.gene39491:intron 25.0%
! TTAATCTTTACATTTCATCC+TGG - chr4.2:22617659-22617678 MS.gene39491:CDS 25.0%
! TTGAATGTTATTCATGAAGA+AGG + chr4.2:22617711-22617730 None:intergenic 25.0%
! TTTATTGAAGTAAACGAGAA+AGG - chr4.2:22618711-22618730 MS.gene39491:CDS 25.0%
! TTTGATTAATGGCTATAATG+TGG - chr4.2:22617604-22617623 MS.gene39491:CDS 25.0%
!! ACATCTTTTCTCAAAAGAAT+CGG - chr4.2:22617098-22617117 MS.gene39491:CDS 25.0%
!! AGTCTAATTTTCACACATTA+CGG + chr4.2:22618001-22618020 None:intergenic 25.0%
!! TATGATTTTCACCTTCTTAA+TGG - chr4.2:22618294-22618313 MS.gene39491:intron 25.0%
!!! TTCTTTTGAGAAAAGATGTT+TGG + chr4.2:22617097-22617116 None:intergenic 25.0%
ACACACTATTGACAAAAAAG+AGG + chr4.2:22617314-22617333 None:intergenic 30.0%
ATGATAGAAAACAACAAGGA+GGG + chr4.2:22617961-22617980 None:intergenic 30.0%
GGATATGATAGAAAACAACA+AGG + chr4.2:22617965-22617984 None:intergenic 30.0%
TATGATAGAAAACAACAAGG+AGG + chr4.2:22617962-22617981 None:intergenic 30.0%
TCTTGCACTTGCTTTATAAA+CGG - chr4.2:22617579-22617598 MS.gene39491:CDS 30.0%
TGTGTTTATCTTGAAGTGAA+AGG - chr4.2:22617820-22617839 MS.gene39491:intron 30.0%
TTTCTTCCAAATGCAAAAGA+TGG - chr4.2:22618444-22618463 MS.gene39491:intron 30.0%
! AATGTGGTTTTTATGGAACT+TGG - chr4.2:22617620-22617639 MS.gene39491:CDS 30.0%
! AGTACTGCATATTTTACCAT+GGG - chr4.2:22618753-22618772 MS.gene39491:CDS 30.0%
! CAAAATCCTACCAATTCTTT+TGG + chr4.2:22617788-22617807 None:intergenic 30.0%
! TAACATTCAAAAGGCATGAA+TGG - chr4.2:22617721-22617740 MS.gene39491:CDS 30.0%
! TCATCTTTTCCTTGCTTATA+AGG + chr4.2:22618402-22618421 None:intergenic 30.0%
! TGATTTTACCTTTCTCTTCT+GGG - chr4.2:22618619-22618638 MS.gene39491:CDS 30.0%
! TGGCTATAATGTGGTTTTTA+TGG - chr4.2:22617613-22617632 MS.gene39491:CDS 30.0%
!! CACTATATTTTAAAGGAGCA+TGG - chr4.2:22618238-22618257 MS.gene39491:intron 30.0%
!! CTTCTGAAATATGTTGCTAA+CGG + chr4.2:22618688-22618707 None:intergenic 30.0%
!! GCTAACGCACTATATTTTAA+AGG - chr4.2:22618231-22618250 MS.gene39491:intron 30.0%
!!! TCTTTTTTGTCAATAGTGTG+TGG - chr4.2:22617313-22617332 MS.gene39491:CDS 30.0%
!!! TTTGATTGACATGGTTTTGA+AGG - chr4.2:22618098-22618117 MS.gene39491:intron 30.0%
AGAAAGGTAAAATCACTCCT+AGG + chr4.2:22618614-22618633 None:intergenic 35.0%
ATAAGCAAGGAAAAGATGAG+CGG - chr4.2:22618403-22618422 MS.gene39491:intron 35.0%
ATATGTTTGAAGGAAGGTTG+AGG + chr4.2:22617357-22617376 None:intergenic 35.0%
ATCAGCTATAAAGTCTAGTC+CGG + chr4.2:22618787-22618806 None:intergenic 35.0%
ATCCTGGCATACAATAAAGT+AGG - chr4.2:22617675-22617694 MS.gene39491:CDS 35.0%
ATTAAAGTTGCTGGTCATGA+AGG + chr4.2:22618337-22618356 None:intergenic 35.0%
CATGGTAAAATATGCAGTAC+TGG + chr4.2:22618754-22618773 None:intergenic 35.0%
GAAATCAACAGAAGACAAAG+TGG + chr4.2:22617400-22617419 None:intergenic 35.0%
TCCTGGCATACAATAAAGTA+GGG - chr4.2:22617676-22617695 MS.gene39491:CDS 35.0%
TCTCATATACTGAAAGACCT+AGG - chr4.2:22618594-22618613 MS.gene39491:CDS 35.0%
TGAGAAAAGATGTTTGGCTA+TGG + chr4.2:22617091-22617110 None:intergenic 35.0%
TTAAAGTTGCTGGTCATGAA+GGG + chr4.2:22618336-22618355 None:intergenic 35.0%
TTTCCCTTCTCCAAAAGAAT+TGG - chr4.2:22617775-22617794 MS.gene39491:CDS 35.0%
! AGTAGCATCTTTGAAGATGA+CGG + chr4.2:22617274-22617293 None:intergenic 35.0%
! ATTTTACCTTTCTCTTCTGG+GGG - chr4.2:22618621-22618640 MS.gene39491:CDS 35.0%
! CAGTACTGCATATTTTACCA+TGG - chr4.2:22618752-22618771 MS.gene39491:CDS 35.0%
! CATTACGGTGCTAAATTAGT+TGG + chr4.2:22617986-22618005 None:intergenic 35.0%
! GATTTTACCTTTCTCTTCTG+GGG - chr4.2:22618620-22618639 MS.gene39491:CDS 35.0%
! GTGATTTTACCTTTCTCTTC+TGG - chr4.2:22618618-22618637 MS.gene39491:CDS 35.0%
!! CTACCAATTCTTTTGGAGAA+GGG + chr4.2:22617781-22617800 None:intergenic 35.0%
!! TTCTGTTGATTTCCAGAACA+AGG - chr4.2:22617407-22617426 MS.gene39491:CDS 35.0%
AAAAGATGGTCTGTCAGCTT+TGG - chr4.2:22618458-22618477 MS.gene39491:intron 40.0%
AAAGATGTTTGGCTATGGTG+AGG + chr4.2:22617086-22617105 None:intergenic 40.0%
AAGATGTTTGGCTATGGTGA+GGG + chr4.2:22617085-22617104 None:intergenic 40.0%
ACCCTACTTTATTGTATGCC+AGG + chr4.2:22617680-22617699 None:intergenic 40.0%
CCTTATAAAACAGTGCGAGT+AGG - chr4.2:22618525-22618544 MS.gene39491:CDS 40.0%
CCTTCTCCAAAAGAATTGGT+AGG - chr4.2:22617779-22617798 MS.gene39491:CDS 40.0%
CTACTTCTCATACCTTGTTC+TGG + chr4.2:22617422-22617441 None:intergenic 40.0%
CTATTGACAAAAAAGAGGCG+AGG + chr4.2:22617309-22617328 None:intergenic 40.0%
CTTCGTCTTCCTTATAAGCA+AGG - chr4.2:22618390-22618409 MS.gene39491:intron 40.0%
GACACGATATGTTTGAAGGA+AGG + chr4.2:22617363-22617382 None:intergenic 40.0%
GGTAGACACGATATGTTTGA+AGG + chr4.2:22617367-22617386 None:intergenic 40.0%
TATAAAACAGTGCGAGTAGG+AGG - chr4.2:22618528-22618547 MS.gene39491:CDS 40.0%
TATAAGGAAGCTTGTGCTCT+AGG + chr4.2:22618512-22618531 None:intergenic 40.0%
TGGGCTAAAAAAGAGACGAA+TGG - chr4.2:22618150-22618169 MS.gene39491:intron 40.0%
TTACTGAGCGTCCATTAAGA+AGG + chr4.2:22618308-22618327 None:intergenic 40.0%
TTTACCATGGGTAGATCAAC+CGG - chr4.2:22618765-22618784 MS.gene39491:CDS 40.0%
! CCTACTCGCACTGTTTTATA+AGG + chr4.2:22618528-22618547 None:intergenic 40.0%
! GACAGACCATCTTTTGCATT+TGG + chr4.2:22618453-22618472 None:intergenic 40.0%
! GATGATCGGTGGATTTGAAT+TGG + chr4.2:22617224-22617243 None:intergenic 40.0%
! TATCGTGTCTACCGATTACA+AGG - chr4.2:22617374-22617393 MS.gene39491:CDS 40.0%
! TCTTAATGGACGCTCAGTAA+AGG - chr4.2:22618308-22618327 MS.gene39491:intron 40.0%
! TTCTGGGGGTTTGACAAAAA+TGG - chr4.2:22618635-22618654 MS.gene39491:CDS 40.0%
! TTTTGTCAATAGTGTGTGGC+TGG - chr4.2:22617317-22617336 MS.gene39491:CDS 40.0%
!! AAAATGGTGGACTCTCTTGA+AGG - chr4.2:22618651-22618670 MS.gene39491:CDS 40.0%
!! AGACAAAGTGGCCTTGTAAT+CGG + chr4.2:22617388-22617407 None:intergenic 40.0%
!! CCTACCAATTCTTTTGGAGA+AGG + chr4.2:22617782-22617801 None:intergenic 40.0%
AAAGATGCTACTCCTTGTCG+TGG - chr4.2:22617282-22617301 MS.gene39491:CDS 45.0%
AAGAGGGTTTAGCACTTGTC+CGG + chr4.2:22618862-22618881 None:intergenic 45.0%
ACCCAAACAATCCTCTTTGC+CGG - chr4.2:22618840-22618859 MS.gene39491:CDS 45.0%
AGCACTACTTGGAGAGACAA+AGG + chr4.2:22617144-22617163 None:intergenic 45.0%
CGATGATTGCAAGCACTACT+TGG + chr4.2:22617155-22617174 None:intergenic 45.0%
GAAGGAAGGTTGAGGAGAAA+GGG + chr4.2:22617349-22617368 None:intergenic 45.0%
GACGAAGGAGTTGAGATGAT+CGG + chr4.2:22617238-22617257 None:intergenic 45.0%
GTGCGTTAGCAAAGATGAGT+CGG + chr4.2:22618221-22618240 None:intergenic 45.0%
TCCGGCAAAGAGGATTGTTT+GGG + chr4.2:22618844-22618863 None:intergenic 45.0%
TGAAGGAAGGTTGAGGAGAA+AGG + chr4.2:22617350-22617369 None:intergenic 45.0%
TGCGTTAGCAAAGATGAGTC+GGG + chr4.2:22618220-22618239 None:intergenic 45.0%
TGGGGGTTTGACAAAAATGG+TGG - chr4.2:22618638-22618657 MS.gene39491:CDS 45.0%
TGGTGAGGGAAACTTTCGTA+TGG + chr4.2:22617071-22617090 None:intergenic 45.0%
! GATCGGTGGATTTGAATTGG+AGG + chr4.2:22617221-22617240 None:intergenic 45.0%
! GGAAATGAGTTGAGAGACGA+AGG + chr4.2:22617253-22617272 None:intergenic 45.0%
! TCTGTCAGCTTTGGTTGAGA+AGG - chr4.2:22618467-22618486 MS.gene39491:intron 45.0%
!! GAAAGGCACTAAAGCTGTTG+CGG - chr4.2:22618728-22618747 MS.gene39491:CDS 45.0%
GAAGGAGTTGAGATGATCGG+TGG + chr4.2:22617235-22617254 None:intergenic 50.0%
GTCAAACCCCCAGAAGAGAA+AGG + chr4.2:22618630-22618649 None:intergenic 50.0%
GTCCGGCAAAGAGGATTGTT+TGG + chr4.2:22618845-22618864 None:intergenic 50.0%
GTCGAGAAGCTGTTGTTGTG+TGG + chr4.2:22617196-22617215 None:intergenic 50.0%
TAGTCCGGTTGATCTACCCA+TGG + chr4.2:22618772-22618791 None:intergenic 50.0%
TTAGCACTTGTCCGGCAAAG+AGG + chr4.2:22618854-22618873 None:intergenic 50.0%
! GTCAGCTTTGGTTGAGAAGG+TGG - chr4.2:22618470-22618489 MS.gene39491:intron 50.0%
GTGCTTGCAATCATCGCTGC+TGG - chr4.2:22617159-22617178 MS.gene39491:CDS 55.0%
!! ATCATCGCTGCTGGCTCTGA+CGG - chr4.2:22617168-22617187 MS.gene39491:CDS 55.0%
AAGAGGCGAGGACCACGACA+AGG + chr4.2:22617297-22617316 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 22617036 22618887 22617036 ID=MS.gene39491
chr4.2 mRNA 22617036 22618887 22617036 ID=MS.gene39491.t1;Parent=MS.gene39491
chr4.2 exon 22618495 22618887 22618495 ID=MS.gene39491.t1.exon1;Parent=MS.gene39491.t1
chr4.2 CDS 22618495 22618887 22618495 ID=cds.MS.gene39491.t1;Parent=MS.gene39491.t1
chr4.2 exon 22617036 22617803 22617036 ID=MS.gene39491.t1.exon2;Parent=MS.gene39491.t1
chr4.2 CDS 22617036 22617803 22617036 ID=cds.MS.gene39491.t1;Parent=MS.gene39491.t1
Gene Sequence

>MS.gene39491

ATGGCGGCTCAGCATGATGAATCAAGCTGCAACCATACGAAAGTTTCCCTCACCATAGCCAAACATCTTTTCTCAAAAGAATCGGAAAAGAATATCGTGTTTTCGCCTTTGTCTCTCCAAGTAGTGCTTGCAATCATCGCTGCTGGCTCTGACGGTTCCACACAACAACAGCTTCTCGACTTCCTCCAATTCAAATCCACCGATCATCTCAACTCCTTCGTCTCTCAACTCATTTCCGTCATCTTCAAAGATGCTACTCCTTGTCGTGGTCCTCGCCTCTTTTTTGTCAATAGTGTGTGGCTGGACAAAACCCTTTCTCCTCAACCTTCCTTCAAACATATCGTGTCTACCGATTACAAGGCCACTTTGTCTTCTGTTGATTTCCAGAACAAGGTATGAGAAGTAGATGATTACAACATCAATTGTTTTTGTGATTTGTAGTTAGCTATATATATATATATATATATATATATATATATATGTCATTATAACGTGTCGCAATCATGAAGTTTTCTGTTTAATTGTTGTAATTGTTCATGCATTTCTTGCACTTGCTTTATAAACGGTGTTTGATTAATGGCTATAATGTGGTTTTTATGGAACTTGGAACTTGAAATTGTTTGTTAATCTTTACATTTCATCCTGGCATACAATAAAGTAGGGTTTTTTTTTCCTTCTTCATGAATAACATTCAAAAGGCATGAATGGAGATACTTAGATGATTCATATTATACTTTTTTTTCCCTTCTCCAAAAGAATTGGTAGGATTTTGAAGTTACAACTTTGTGTTTATCTTGAAGTGAAAGGTAATTAGTATTAATCTTGTTGTCTCTTTTAGCATACAACTATTCAAGACTGAAATGATTTTAGAGTAGCAAGTATCAACTTCTACGTAAACAAACAATTGACGAATGAAACTTTTCCCTCCTTGTTGTTTTCTATCATATCCAACTAATTTAGCACCGTAATGTGTGAAAATTAGACTAGCAGAAAGTTGATTTCTTCTATTGAAATACTTGTGAAGAGAATTCAATTGATCTATTTGCATAACTGATATGTAAATTTGATTGACATGGTTTTGAAGGCAACTGAAGTGACAAAAGAAGTGAATTTATGGGCTAAAAAAGAGACGAATGGCCTTATTAAAAAAATTGTTGCTCAAGGGTCAGTCGACAGCTTAACCCGACTCATCTTTGCTAACGCACTATATTTTAAAGGAGCATGGATTCAATCGTTTCCTAAATATATGACAAAAAAATATGATTTTCACCTTCTTAATGGACGCTCAGTAAAGGTTCCCTTCATGACCAGCAACTTTAATCAATTTATTAGAGTTTTTGATGGTTTTAAAGTTCTTCGTCTTCCTTATAAGCAAGGAAAAGATGAGCGGAAATTCTCTATGTACATTTTTCTTCCAAATGCAAAAGATGGTCTGTCAGCTTTGGTTGAGAAGGTGGCTTCTGAATTTGAGTTCCTAGAGCACAAGCTTCCTTATAAAACAGTGCGAGTAGGAGGCTTCAAAATTCCAAGATTCAAATTTTCATCTAGACTTGAAACTTCTCATATACTGAAAGACCTAGGAGTGATTTTACCTTTCTCTTCTGGGGGTTTGACAAAAATGGTGGACTCTCTTGAAGGTCAAAATCTCTCCGTTAGCAACATATTTCAGAAGTGTTTTATTGAAGTAAACGAGAAAGGCACTAAAGCTGTTGCGGCCAGTACTGCATATTTTACCATGGGTAGATCAACCGGACTAGACTTTATAGCTGATCACCCTTTCTTATTTTTAATTAGAGAAGATTTGACCCAAACAATCCTCTTTGCCGGACAAGTGCTAAACCCTCTTTGCTGA
Protein sequence

>MS.gene39491.t1

MAAQHDESSCNHTKVSLTIAKHLFSKESEKNIVFSPLSLQVVLAIIAAGSDGSTQQQLLDFLQFKSTDHLNSFVSQLISVIFKDATPCRGPRLFFVNSVWLDKTLSPQPSFKHIVSTDYKATLSSVDFQNKATEVTKEVNLWAKKETNGLIKKIVAQGSVDSLTRLIFANALYFKGAWIQSFPKYMTKKYDFHLLNGRSVKVPFMTSNFNQFIRVFDGFKVLRLPYKQGKDERKFSMYIFLPNAKDGLSALVEKVASEFEFLEHKLPYKTVRVGGFKIPRFKFSSRLETSHILKDLGVILPFSSGGLTKMVDSLEGQNLSVSNIFQKCFIEVNEKGTKAVAASTAYFTMGRSTGLDFIADHPFLFLIREDLTQTILFAGQVLNPLC