AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39581.t1 MTR_4g087420 94.664 506 21 2 6 508 32 534 0.0 963
MS.gene39581.t1 MTR_6g033415 35.652 345 179 12 114 448 101 412 1.10e-39 149
MS.gene39581.t1 MTR_7g100790 34.270 356 183 11 120 448 108 439 2.14e-37 143
MS.gene39581.t1 MTR_1g066800 31.638 354 188 10 118 448 114 436 9.12e-35 136
MS.gene39581.t1 MTR_1g066800 31.638 354 188 10 118 448 119 441 1.12e-34 136
MS.gene39581.t1 MTR_0047s0040 26.697 442 245 14 71 496 49 427 6.96e-33 130
MS.gene39581.t1 MTR_1g095740 37.838 185 106 4 118 301 139 315 6.46e-32 128
MS.gene39581.t1 MTR_8g024540 30.079 379 195 11 119 446 126 485 7.28e-31 126
MS.gene39581.t1 MTR_7g114700 28.529 340 180 10 116 450 131 412 9.66e-29 118
MS.gene39581.t1 MTR_4g050480 34.018 341 169 17 122 453 99 392 3.39e-24 105
MS.gene39581.t1 MTR_4g050480 34.018 341 169 17 122 453 98 391 3.52e-24 105
MS.gene39581.t1 MTR_2g078210 31.892 185 106 5 111 290 65 234 2.11e-20 92.4
MS.gene39581.t1 MTR_2g078210 31.892 185 106 5 111 290 5 174 2.40e-20 90.9
MS.gene39581.t1 MTR_2g015370 32.961 179 105 7 121 298 64 228 4.81e-19 88.2
MS.gene39581.t1 MTR_3g104570 27.596 366 200 12 85 419 55 386 5.93e-19 89.7
MS.gene39581.t1 MTR_1g007920 31.250 320 155 10 122 416 99 378 8.90e-18 86.3
MS.gene39581.t1 MTR_1g007920 31.250 320 155 10 122 416 124 403 1.75e-17 85.5
MS.gene39581.t1 MTR_0019s0070 43.038 79 45 0 116 194 113 191 3.05e-16 81.6
MS.gene39581.t1 MTR_3g020990 32.624 141 81 2 133 273 103 229 3.49e-16 78.6
MS.gene39581.t1 MTR_3g091560 27.532 316 163 8 122 428 114 372 7.21e-15 77.0
MS.gene39581.t1 MTR_1g101330 44.776 67 37 0 119 185 114 180 3.65e-14 75.1
MS.gene39581.t1 MTR_8g102400 41.667 84 43 1 118 195 110 193 1.61e-13 72.8
MS.gene39581.t1 MTR_1g051810 31.206 141 86 3 121 256 106 240 3.03e-13 71.6
MS.gene39581.t1 MTR_8g074730 50.000 62 31 0 122 183 73 134 3.47e-13 67.4
MS.gene39581.t1 MTR_7g114870 46.970 66 35 0 122 187 108 173 4.73e-13 71.2
MS.gene39581.t1 MTR_4g108140 48.387 62 32 0 122 183 101 162 6.35e-13 71.6
MS.gene39581.t1 MTR_4g108140 48.387 62 32 0 122 183 102 163 6.42e-13 71.6
MS.gene39581.t1 MTR_2g100740 51.613 62 30 0 121 182 98 159 1.23e-12 69.7
MS.gene39581.t1 MTR_4g102840 50.820 61 30 0 122 182 90 150 1.20e-11 67.0
MS.gene39581.t1 MTR_8g014580 45.902 61 33 0 122 182 128 188 1.44e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39581.t1 AT3G59690 50.984 508 172 17 26 508 62 517 2.76e-140 414
MS.gene39581.t1 AT3G59690 50.984 508 172 17 26 508 62 517 2.76e-140 414
MS.gene39581.t1 AT2G43680 52.772 451 139 16 66 508 284 668 2.72e-124 378
MS.gene39581.t1 AT2G43680 52.772 451 139 16 66 508 284 668 2.72e-124 378
MS.gene39581.t1 AT2G43680 52.772 451 139 16 66 508 284 668 2.72e-124 378
MS.gene39581.t1 AT2G43680 52.772 451 139 16 66 508 284 668 2.72e-124 378
MS.gene39581.t1 AT2G43680 52.772 451 139 16 66 508 285 669 2.74e-124 378
MS.gene39581.t1 AT5G03040 34.167 360 169 13 118 448 113 433 4.35e-40 151
MS.gene39581.t1 AT5G03040 34.167 360 169 13 118 448 113 433 4.35e-40 151
MS.gene39581.t1 AT5G03040 34.167 360 169 13 118 448 113 433 4.35e-40 151
MS.gene39581.t1 AT3G52290 33.876 307 169 10 119 418 107 386 3.97e-39 147
MS.gene39581.t1 AT5G13460 29.819 332 163 10 92 423 90 351 8.39e-29 119
MS.gene39581.t1 AT1G01110 28.266 421 228 16 46 423 58 447 1.62e-28 119
MS.gene39581.t1 AT3G49260 29.470 302 174 6 122 415 122 392 7.04e-27 114
MS.gene39581.t1 AT3G49260 29.470 302 174 6 122 415 122 392 7.04e-27 114
MS.gene39581.t1 AT3G49260 29.470 302 174 6 122 415 122 392 7.04e-27 114
MS.gene39581.t1 AT3G49260 28.477 302 178 5 122 415 122 393 1.02e-26 113
MS.gene39581.t1 AT2G26410 37.640 178 92 6 119 290 147 311 4.61e-26 112
MS.gene39581.t1 AT2G26410 37.640 178 92 6 119 290 147 311 4.61e-26 112
MS.gene39581.t1 AT4G00820 31.044 364 195 15 119 447 131 473 6.48e-25 108
MS.gene39581.t1 AT3G09710 38.012 171 95 4 119 288 107 267 1.42e-24 106
MS.gene39581.t1 AT3G09710 36.517 178 102 4 119 288 107 281 1.63e-23 103
MS.gene39581.t1 AT2G26180 34.314 204 114 6 122 315 86 279 4.78e-23 101
MS.gene39581.t1 AT3G22190 38.710 186 89 6 122 299 90 258 1.55e-22 100
MS.gene39581.t1 AT3G22190 38.710 186 89 6 122 299 90 258 1.55e-22 100
MS.gene39581.t1 AT3G22190 38.710 186 89 6 122 299 90 258 1.55e-22 100
MS.gene39581.t1 AT3G22190 38.710 186 89 6 122 299 90 258 1.55e-22 100
MS.gene39581.t1 AT2G33990 32.579 221 120 9 122 337 49 245 4.11e-22 96.3
MS.gene39581.t1 AT1G72670 31.959 194 107 6 116 300 89 266 4.75e-18 86.7
MS.gene39581.t1 AT1G17480 36.296 135 67 2 122 256 96 211 5.65e-18 86.3
MS.gene39581.t1 AT3G15050 32.821 195 111 7 105 290 27 210 1.18e-17 83.2
MS.gene39581.t1 AT4G10640 33.333 165 83 4 114 257 94 252 1.50e-17 85.5
MS.gene39581.t1 AT5G03960 35.036 137 84 1 120 256 108 239 1.15e-14 76.3
MS.gene39581.t1 AT5G03960 35.036 137 84 1 120 256 108 239 1.15e-14 76.3
MS.gene39581.t1 AT5G03960 35.036 137 84 1 120 256 107 238 1.29e-14 76.3
MS.gene39581.t1 AT4G14750 45.588 68 37 0 119 186 104 171 7.87e-14 73.6
MS.gene39581.t1 AT4G14750 45.588 68 37 0 119 186 163 230 1.21e-13 73.6
MS.gene39581.t1 AT4G14750 47.692 65 34 0 122 186 166 230 1.28e-13 73.2
MS.gene39581.t1 AT1G18840 44.615 65 36 0 119 183 108 172 2.14e-13 73.2
MS.gene39581.t1 AT1G18840 44.615 65 36 0 119 183 108 172 2.14e-13 73.2
MS.gene39581.t1 AT1G18840 44.615 65 36 0 119 183 108 172 2.14e-13 73.2
MS.gene39581.t1 AT3G16490 48.387 62 32 0 122 183 109 170 3.30e-13 71.6
MS.gene39581.t1 AT5G62070 51.786 56 27 0 119 174 116 171 3.45e-13 71.6
MS.gene39581.t1 AT5G07240 46.774 62 33 0 119 180 105 166 4.59e-13 71.2
MS.gene39581.t1 AT5G07240 46.774 62 33 0 119 180 151 212 5.44e-13 71.2
MS.gene39581.t1 AT3G49380 37.634 93 55 1 119 208 109 201 1.72e-12 69.3
MS.gene39581.t1 AT1G74690 27.811 169 85 4 120 283 113 249 2.24e-12 69.7
MS.gene39581.t1 AT1G51960 42.857 77 39 1 122 198 102 173 5.59e-12 67.8
MS.gene39581.t1 AT3G51380 42.857 70 40 0 114 183 31 100 6.04e-12 62.8
MS.gene39581.t1 AT4G29150 43.750 64 36 0 119 182 130 193 8.77e-12 67.4
MS.gene39581.t1 AT1G01110 25.085 295 172 12 165 423 3 284 9.19e-11 63.9

Find 145 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGCATTGAGAAAATATT+TGG 0.147656 4.2:-23958255 MS.gene39581:CDS
ATAGTCGATCTTGTTGATTT+TGG 0.221689 4.2:+23956592 None:intergenic
AATCACACAAATCTTGTAAA+AGG 0.233035 4.2:+23956094 None:intergenic
GGCTTTCTTTCAACCCTTGC+AGG 0.240511 4.2:+23956115 None:intergenic
ACAGGCGTTTGAGTTGATTT+TGG 0.283729 4.2:+23956817 None:intergenic
TTTCCTTGTGGAGTTCTAAA+TGG 0.286373 4.2:+23956538 None:intergenic
TCAAGTGACTGGTATTTATC+TGG 0.291478 4.2:+23956178 None:intergenic
GTCAATGTTCTCTAGAAATA+AGG 0.300536 4.2:-23956224 MS.gene39581:CDS
TTTCCTTTGTCACAAGGAAT+AGG 0.310206 4.2:-23956268 MS.gene39581:CDS
GGGTCATTCAAATGGAATAA+AGG 0.327034 4.2:-23956247 MS.gene39581:CDS
GTGTGAATTGGAAATTCTTC+AGG 0.333924 4.2:+23956707 None:intergenic
CTCTTGGAATCAGTGCTTGT+TGG 0.334476 4.2:+23956301 None:intergenic
TGAGAGCTTGAGCCAGGTCT+TGG 0.341364 4.2:+23956652 None:intergenic
TACCAGTCACTTGAATCTAT+TGG 0.350119 4.2:-23956169 MS.gene39581:CDS
ACAGTTGGAGCCATGTAGTT+TGG 0.351130 4.2:+23956400 None:intergenic
GGCTGCTTACAGAGGTTATA+TGG 0.351906 4.2:-23957959 MS.gene39581:intron
CACACCACCAAGACCATATT+CGG 0.361031 4.2:-23956689 MS.gene39581:CDS
TTCCTTTGTCACAAGGAATA+GGG 0.361875 4.2:-23956267 MS.gene39581:CDS
CAGAGCTCTAAAACTCTTCC+TGG 0.366223 4.2:+23957642 None:intergenic
ACCTTTCGACGTACCATTGA+AGG 0.378126 4.2:-23956461 MS.gene39581:CDS
TTCCGCGAAGATGCCGCTTT+CGG 0.379549 4.2:+23958060 None:intergenic
GCAGCATTGAGAAAATATTT+GGG 0.385983 4.2:-23958254 MS.gene39581:CDS
TGGTTCTGTAAAGTTGGTTC+TGG 0.389882 4.2:+23958006 None:intergenic
TAACAGCTGCATGTGTTTCA+TGG 0.394012 4.2:+23957555 None:intergenic
ATGGTCTTGGTGGTGTGAAT+TGG 0.398616 4.2:+23956695 None:intergenic
GGTCATTCAAATGGAATAAA+GGG 0.400507 4.2:-23956246 MS.gene39581:CDS
CTAAATGGAGGAGTTCTAGA+TGG 0.403634 4.2:+23956553 None:intergenic
GGCCTTTGAGAGCTTGAGCC+AGG 0.404316 4.2:+23956646 None:intergenic
AAAGGTTTGGTAAGGCTTCA+AGG 0.407441 4.2:-23957619 MS.gene39581:CDS
AATGAAGCTGGAAGTACCTT+GGG 0.409619 4.2:-23957454 MS.gene39581:CDS
TGCTGCTGAGATGGGAAAGA+AGG 0.411748 4.2:-23959223 MS.gene39581:CDS
AACAACAAGTACTTGCAATT+AGG 0.413110 4.2:-23958218 MS.gene39581:CDS
TGGTACGTCGAAAGGTGAAT+TGG 0.417209 4.2:+23956468 None:intergenic
CCACCGCCAAATCTTTCTCT+TGG 0.423496 4.2:+23956343 None:intergenic
ACCTTGGGGAAAAGTGCTTT+AGG 0.429209 4.2:-23957439 MS.gene39581:CDS
AGGCAGGTAACAATGAAGAT+TGG 0.438407 4.2:-23957244 MS.gene39581:CDS
AGTAATCCAAGAGAAAGATT+TGG 0.440973 4.2:-23956349 MS.gene39581:CDS
TTTGGTCGCTCGGGAGGTGT+GGG 0.442251 4.2:+23958192 None:intergenic
AAGGAATAGGGTCATTCAAA+TGG 0.447925 4.2:-23956255 MS.gene39581:CDS
GCCCTTGGAGATGGATCCCT+TGG 0.448654 4.2:+23958105 None:intergenic
ACTCGGCTTGATATCGTGCC+TGG 0.450049 4.2:+23957482 None:intergenic
GGCATTTGCATATTCACATC+AGG 0.465718 4.2:-23957138 MS.gene39581:intron
AAACAGGAATGCTGCTGAGA+TGG 0.468508 4.2:-23959232 None:intergenic
GGCAGGTAACAATGAAGATT+GGG 0.469502 4.2:-23957243 MS.gene39581:CDS
GGCTTAGGAGAAGGAGGCCT+AGG 0.481446 4.2:+23958135 None:intergenic
TCCAGCTTCATTCTTGTACT+CGG 0.482184 4.2:+23957465 None:intergenic
AGGAAACGAGACTCTCCTCT+TGG 0.484468 4.2:+23956285 None:intergenic
GATTCCCATGGTGGTGGAAC+TGG 0.486021 4.2:-23956774 MS.gene39581:CDS
TCCTTCAATGGTACGTCGAA+AGG 0.487272 4.2:+23956460 None:intergenic
GAGCTCTGAAAGGTTTGGTA+AGG 0.487495 4.2:-23957627 MS.gene39581:CDS
GCGTGACGTCAAAATATTCA+AGG 0.487546 4.2:-23956507 MS.gene39581:CDS
CTCCGAAAGCGGCATCTTCG+CGG 0.487625 4.2:-23958062 MS.gene39581:CDS
GGAGAAGCGGCCCTTGGAGA+TGG 0.488016 4.2:+23958096 None:intergenic
AGATGGGAAAGAAGGGGAGT+TGG 0.488180 4.2:-23959215 MS.gene39581:CDS
TCTTTCAACCCTTGCAGGCA+AGG 0.489990 4.2:+23956120 None:intergenic
TCCCTTGGAGAAGGAGGCTT+AGG 0.492264 4.2:+23958120 None:intergenic
TGTCTCGGCGACTAAGATCC+AGG 0.493274 4.2:-23957980 MS.gene39581:CDS
CTCCTAAGCCTCCTTCTCCA+AGG 0.498883 4.2:-23958122 MS.gene39581:CDS
AACAGGAATGCTGCTGAGAT+GGG 0.501119 4.2:-23959231 None:intergenic
GCCGAGTACAAGAATGAAGC+TGG 0.501793 4.2:-23957466 MS.gene39581:CDS
CTCCTAGAGTTGCTTCTCCT+AGG 0.502361 4.2:-23958152 MS.gene39581:CDS
ACATGCTGGTTCTGTAAAGT+TGG 0.510843 4.2:+23958000 None:intergenic
ACTCGCGGAGAAGCGGCCCT+TGG 0.513311 4.2:+23958090 None:intergenic
GAATGAAGCTGGAAGTACCT+TGG 0.515373 4.2:-23957455 MS.gene39581:CDS
AGGCTTCAAGGAGTGGTAAG+AGG 0.518282 4.2:-23957607 MS.gene39581:CDS
GGTCGCTCGGGAGGTGTGGG+AGG 0.522529 4.2:+23958195 None:intergenic
AGACTGTCATCGTCCTTCAA+TGG 0.522835 4.2:+23956448 None:intergenic
TATTCAAGGCCAAGGGGAGT+AGG 0.527231 4.2:-23956493 MS.gene39581:CDS
CCAACCAGTTCCACCACCAT+GGG 0.530319 4.2:+23956770 None:intergenic
GAATTGGATCCTACTCCCCT+TGG 0.530551 4.2:+23956484 None:intergenic
CTCGATCGCGTATCTGTCAC+AGG 0.530625 4.2:+23956799 None:intergenic
GACCCTATTCCTTGTGACAA+AGG 0.531331 4.2:+23956265 None:intergenic
TACACATCACTCTAAGGGAA+AGG 0.537172 4.2:-23959169 MS.gene39581:intron
CGTCAAAATATTCAAGGCCA+AGG 0.538050 4.2:-23956501 MS.gene39581:CDS
TTCCAATAGATTCAAGTGAC+TGG 0.538161 4.2:+23956167 None:intergenic
GCTGCTGAGATGGGAAAGAA+GGG 0.540744 4.2:-23959222 MS.gene39581:CDS
TCCTAAGCCTCCTTCTCCAA+GGG 0.542552 4.2:-23958121 MS.gene39581:CDS
AGCAGTATCCTTGCCTGCAA+GGG 0.545645 4.2:-23956128 MS.gene39581:CDS
ATTACTACTAGCGCGTACTT+TGG 0.546568 4.2:+23956366 None:intergenic
AGAAGCGAGGTTGCAGAGGA+AGG 0.547317 4.2:-23957192 MS.gene39581:CDS
TCCATGTTATCAAAGGCAAA+AGG 0.548096 4.2:+23956625 None:intergenic
CGAGCGGATTCCCATGGTGG+TGG 0.550081 4.2:-23956780 MS.gene39581:CDS
TCTTAGTCGCCGAGACATGC+TGG 0.550419 4.2:+23957986 None:intergenic
TTGGCCTTGGCAGAAACAGT+TGG 0.550995 4.2:+23956385 None:intergenic
TGATAGCTTACTGACAAAAG+AGG 0.552866 4.2:-23957219 MS.gene39581:CDS
ACGCTTGAAGTGTTTCCTTG+TGG 0.554504 4.2:+23956526 None:intergenic
AAGTAGAAGCGAGGTTGCAG+AGG 0.554875 4.2:-23957196 MS.gene39581:CDS
GTCTCGTTTCCTTTGTCACA+AGG 0.555248 4.2:-23956274 MS.gene39581:CDS
CAGCAGTATCCTTGCCTGCA+AGG 0.555494 4.2:-23956129 MS.gene39581:CDS
GATGGATCCCTTGGAGAAGG+AGG 0.557622 4.2:+23958114 None:intergenic
AATCCAGATGTTAGAAAACC+AGG 0.559159 4.2:-23957500 MS.gene39581:CDS
ACTAGCGCGTACTTTGGCCT+TGG 0.561095 4.2:+23956372 None:intergenic
AGGATTCTCATGGCTCTCAC+AGG 0.561873 4.2:-23956204 MS.gene39581:CDS
TGTTCCGAATATGGTCTTGG+TGG 0.562203 4.2:+23956685 None:intergenic
CTAGGAGAAGCAACTCTAGG+AGG 0.564157 4.2:+23958153 None:intergenic
TATAACCTCTGTAAGCAGCC+TGG 0.564353 4.2:+23957962 None:intergenic
GGCTCCAACTGTTTCTGCCA+AGG 0.565988 4.2:-23956389 MS.gene39581:CDS
CATCAAAAGAGAGAGATCAA+TGG 0.567226 4.2:-23957159 MS.gene39581:CDS
CCTTGTGGAGTTCTAAATGG+AGG 0.567299 4.2:+23956541 None:intergenic
AGATTTGGCGGTGGTAGTAG+TGG 0.568746 4.2:-23956334 MS.gene39581:CDS
ATGTAGTTTGGAACGGAGAA+CGG 0.569098 4.2:+23956412 None:intergenic
GGGAAAAGTGCTTTAGGACA+TGG 0.569234 4.2:-23957433 MS.gene39581:CDS
GTCAAAATATTCAAGGCCAA+GGG 0.570622 4.2:-23956500 MS.gene39581:CDS
TTTGGTAAGGCTTCAAGGAG+TGG 0.571343 4.2:-23957614 MS.gene39581:CDS
AATCCAAGAGAAAGATTTGG+CGG 0.577080 4.2:-23956346 MS.gene39581:CDS
GGAGATGGATCCCTTGGAGA+AGG 0.577659 4.2:+23958111 None:intergenic
CTCCAAGGGATCCATCTCCA+AGG 0.580760 4.2:-23958107 MS.gene39581:CDS
AGAGGTAACTCGCGGAGAAG+CGG 0.585392 4.2:+23958083 None:intergenic
TCCAACCAGTTCCACCACCA+TGG 0.585925 4.2:+23956769 None:intergenic
TGGTGTATCCATGTTATCAA+AGG 0.590388 4.2:+23956618 None:intergenic
GGAGAAGGAGGCTTAGGAGA+AGG 0.593364 4.2:+23958126 None:intergenic
TGGAGCCATGTAGTTTGGAA+CGG 0.593443 4.2:+23956405 None:intergenic
GGCCTAGGAGAAGCAACTCT+AGG 0.595533 4.2:+23958150 None:intergenic
GACCTGGCTCAAGCTCTCAA+AGG 0.598818 4.2:-23956648 MS.gene39581:CDS
TCCAAGGGATCCATCTCCAA+GGG 0.601031 4.2:-23958106 MS.gene39581:CDS
TAGGAGAAGCAACTCTAGGA+GGG 0.605104 4.2:+23958154 None:intergenic
CCTCCATTTAGAACTCCACA+AGG 0.606509 4.2:-23956541 MS.gene39581:CDS
GAAGGAGGCTTAGGAGAAGG+AGG 0.609274 4.2:+23958129 None:intergenic
TTTACAGAACCAGCATGTCT+CGG 0.610936 4.2:-23957995 MS.gene39581:CDS
TCTAGAAATAAGGATTCTCA+TGG 0.612036 4.2:-23956214 MS.gene39581:CDS
GTTCTCCGTTCCAAACTACA+TGG 0.616839 4.2:-23956410 MS.gene39581:CDS
CGCGATCGAGCGGATTCCCA+TGG 0.617077 4.2:-23956786 MS.gene39581:CDS
ATCTATTGGAAATGTGAGTG+TGG 0.620035 4.2:-23956155 MS.gene39581:CDS
ACTCTAGGAGGGACGAAAGG+TGG 0.620082 4.2:+23958165 None:intergenic
ACTATGTTAACAGTTATGGA+AGG 0.620863 4.2:-23956845 MS.gene39581:intron
CCAAGAGAAAGATTTGGCGG+TGG 0.631411 4.2:-23956343 MS.gene39581:CDS
CAAAAGACAAGTCCAAGACC+TGG 0.632412 4.2:-23956664 MS.gene39581:CDS
CAGCATTGAGAAAATATTTG+GGG 0.632868 4.2:-23958253 MS.gene39581:CDS
GCGAGTTACCTCTCCGAAAG+CGG 0.633756 4.2:-23958073 MS.gene39581:CDS
GATCGAGCGGATTCCCATGG+TGG 0.638826 4.2:-23956783 MS.gene39581:CDS
GCAACTCTAGGAGGGACGAA+AGG 0.644669 4.2:+23958162 None:intergenic
AAGATCCAGGCTGCTTACAG+AGG 0.646831 4.2:-23957967 MS.gene39581:CDS
TAAGAGGACAGAATGTGAAG+AGG 0.651089 4.2:-23957591 MS.gene39581:CDS
TCAAAATATTCAAGGCCAAG+GGG 0.659140 4.2:-23956499 MS.gene39581:CDS
CAACAAGCACTGATTCCAAG+AGG 0.666446 4.2:-23956300 MS.gene39581:CDS
GTGACAGATACGCGATCGAG+CGG 0.669089 4.2:-23956796 MS.gene39581:CDS
CCCATGGTGGTGGAACTGGT+TGG 0.672536 4.2:-23956770 MS.gene39581:CDS
CGAAGATGCCGCTTTCGGAG+AGG 0.682021 4.2:+23958065 None:intergenic
ATGAAGCTGGAAGTACCTTG+GGG 0.697268 4.2:-23957453 MS.gene39581:CDS
GCGGAATGTTCATCAACACA+AGG 0.697723 4.2:-23958043 MS.gene39581:CDS
CTGCTGAGATGGGAAAGAAG+GGG 0.698156 4.2:-23959221 MS.gene39581:CDS
CAAAAGAGGAAGTAGAAGCG+AGG 0.699187 4.2:-23957205 MS.gene39581:CDS
TAGTTTGGAACGGAGAACGG+TGG 0.711322 4.2:+23956415 None:intergenic
GCTTTCGGAGAGGTAACTCG+CGG 0.724827 4.2:+23958075 None:intergenic
GAATGTTCATCAACACAAGG+AGG 0.727547 4.2:-23958040 MS.gene39581:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTCTTAATATTATCAA+AGG + chr4.2:23956521-23956540 None:intergenic 10.0%
!!! TTTTATTTATGCTTTTATTT+TGG - chr4.2:23958228-23958247 MS.gene39581:CDS 10.0%
!! AAAAATCTTAGATTTAGAAA+TGG + chr4.2:23958308-23958327 None:intergenic 15.0%
!! AAAATCTTAGATTTAGAAAT+GGG + chr4.2:23958307-23958326 None:intergenic 15.0%
!! CTAATTTATTTGTGATAATT+TGG - chr4.2:23958036-23958055 MS.gene39581:CDS 15.0%
!! TAAGATAAGTATTAAGTATT+TGG - chr4.2:23957547-23957566 MS.gene39581:CDS 15.0%
!!! AATTAGTCTGATTTATATAA+AGG + chr4.2:23958022-23958041 None:intergenic 15.0%
!!! ATTATTTGAAATATTGCAAA+AGG - chr4.2:23956679-23956698 MS.gene39581:CDS 15.0%
!!! ATTTTTGTTTCGATTAAAAT+AGG + chr4.2:23958275-23958294 None:intergenic 15.0%
!!! GTTACTTTATATAAAATTGA+TGG + chr4.2:23957943-23957962 None:intergenic 15.0%
!! AAAAAAGCTGGTAAAATTAA+TGG + chr4.2:23957601-23957620 None:intergenic 20.0%
!! AAAGCATAAATAAAAAGTCT+AGG + chr4.2:23958223-23958242 None:intergenic 20.0%
!! ATAACTTAAAACAACTTCAA+AGG + chr4.2:23956875-23956894 None:intergenic 20.0%
!! TCATAAAAAATTAGCTAAAC+TGG + chr4.2:23958371-23958390 None:intergenic 20.0%
!!! AAGTATTTGGTTTCTAAATT+TGG - chr4.2:23957560-23957579 MS.gene39581:CDS 20.0%
!!! TCTTATAATTGGTTTTCTTT+TGG - chr4.2:23956260-23956279 MS.gene39581:CDS 20.0%
! ATATGTGTCATATCCTTAAA+TGG + chr4.2:23956308-23956327 None:intergenic 25.0%
! ATGATGTAAGATAAGAATGA+TGG + chr4.2:23956731-23956750 None:intergenic 25.0%
! ATTAAGCATTTCACATTTCT+CGG + chr4.2:23956211-23956230 None:intergenic 25.0%
! CCAAAATTGTTAACCATTTA+AGG - chr4.2:23956292-23956311 MS.gene39581:CDS 25.0%
! CTTAATACTTATCTTAGATG+AGG + chr4.2:23957543-23957562 None:intergenic 25.0%
! GAAATATTGCAAAAGGATTT+AGG - chr4.2:23956686-23956705 MS.gene39581:CDS 25.0%
! GACAAAAAAAATTAAGATGC+AGG + chr4.2:23956624-23956643 None:intergenic 25.0%
! TAGCATATTCATTAATGATG+TGG - chr4.2:23958393-23958412 MS.gene39581:intron 25.0%
! TGATGTAAGATAAGAATGAT+GGG + chr4.2:23956730-23956749 None:intergenic 25.0%
! TTAATAAGCATGATTAGACT+AGG + chr4.2:23956941-23956960 None:intergenic 25.0%
! TTTGTTTCGATTAAAATAGG+TGG + chr4.2:23958272-23958291 None:intergenic 25.0%
!! CCTTAAATGGTTAACAATTT+TGG + chr4.2:23956295-23956314 None:intergenic 25.0%
!!! AATAGAATTTAGCCTTTTTG+AGG - chr4.2:23957415-23957434 MS.gene39581:intron 25.0%
!!! AATCAACCGAAATTTTGATA+CGG + chr4.2:23956578-23956597 None:intergenic 25.0%
!!! TCATTATTTTACTACGAATC+TGG - chr4.2:23956833-23956852 MS.gene39581:CDS 25.0%
!!! TTTTTTGTCAATAACAGTTC+AGG + chr4.2:23956458-23956477 None:intergenic 25.0%
AAATTAGCTAAACTGGATTG+TGG + chr4.2:23958364-23958383 None:intergenic 30.0%
AACAACAAGTACTTGCAATT+AGG - chr4.2:23957102-23957121 MS.gene39581:intron 30.0%
AAGTACCACATATAAGAGTT+AGG + chr4.2:23957646-23957665 None:intergenic 30.0%
AATTCCCGAATATGTTTGTA+AGG + chr4.2:23957447-23957466 None:intergenic 30.0%
ACTATGTTAACAGTTATGGA+AGG - chr4.2:23958475-23958494 MS.gene39581:intron 30.0%
AGTAATCCAAGAGAAAGATT+TGG - chr4.2:23958971-23958990 MS.gene39581:intron 30.0%
AGTATCCTAACTCTTATATG+TGG - chr4.2:23957638-23957657 MS.gene39581:CDS 30.0%
ATCATGCTTATTAACATAGC+AGG - chr4.2:23956947-23956966 MS.gene39581:intron 30.0%
ATGTGGTACTTGCAATATAT+AGG - chr4.2:23957655-23957674 MS.gene39581:intron 30.0%
CTTGTACCGTATCAAAATTT+CGG - chr4.2:23956569-23956588 MS.gene39581:CDS 30.0%
GGACCTTGATATGAATTAAA+AGG + chr4.2:23956603-23956622 None:intergenic 30.0%
GGTCATTCAAATGGAATAAA+GGG - chr4.2:23959074-23959093 MS.gene39581:intron 30.0%
GTATCAAAATTTCGGTTGAT+TGG - chr4.2:23956577-23956596 MS.gene39581:CDS 30.0%
GTTCTCCTTACAAACATATT+CGG - chr4.2:23957439-23957458 MS.gene39581:CDS 30.0%
GTTGACTATGTTAACAGTTA+TGG - chr4.2:23958471-23958490 MS.gene39581:intron 30.0%
TCTAGAAATAAGGATTCTCA+TGG - chr4.2:23959106-23959125 MS.gene39581:intron 30.0%
TGGAATGTTCATAACTCATA+AGG + chr4.2:23956547-23956566 None:intergenic 30.0%
TTCATGATCGATAAAAAAGC+TGG + chr4.2:23957613-23957632 None:intergenic 30.0%
TTCTCCTTACAAACATATTC+GGG - chr4.2:23957440-23957459 MS.gene39581:CDS 30.0%
! AATATTTTCTCAATGCTGCT+TGG + chr4.2:23957065-23957084 None:intergenic 30.0%
! AGCAGCATTGAGAAAATATT+TGG - chr4.2:23957065-23957084 MS.gene39581:intron 30.0%
! CAGCATTGAGAAAATATTTG+GGG - chr4.2:23957067-23957086 MS.gene39581:intron 30.0%
! GCAGCATTGAGAAAATATTT+GGG - chr4.2:23957066-23957085 MS.gene39581:intron 30.0%
! GGTAAAATTAATGGATCAAG+CGG + chr4.2:23957592-23957611 None:intergenic 30.0%
! GTCAATGTTCTCTAGAAATA+AGG - chr4.2:23959096-23959115 MS.gene39581:intron 30.0%
! GTTTTTACACATCACTCTAA+GGG - chr4.2:23956146-23956165 MS.gene39581:CDS 30.0%
! TGGAATTTTTGTAAAGTCTG+AGG - chr4.2:23958056-23958075 MS.gene39581:CDS 30.0%
!! TGGCCTTTTAATTCATATCA+AGG - chr4.2:23956597-23956616 MS.gene39581:CDS 30.0%
!!! ATAGTCGATCTTGTTGATTT+TGG + chr4.2:23958731-23958750 None:intergenic 30.0%
AAGGAATAGGGTCATTCAAA+TGG - chr4.2:23959065-23959084 MS.gene39581:intron 35.0%
AATCCAAGAGAAAGATTTGG+CGG - chr4.2:23958974-23958993 MS.gene39581:intron 35.0%
AATCCAGATGTTAGAAAACC+AGG - chr4.2:23957820-23957839 MS.gene39581:intron 35.0%
ACAGTCCATTCTCATCAAAT+TGG + chr4.2:23956488-23956507 None:intergenic 35.0%
AGATTCAAACCATGTTCTTG+AGG + chr4.2:23956332-23956351 None:intergenic 35.0%
AGCACAAAAGAGAAGAAGAA+AGG - chr4.2:23956984-23957003 MS.gene39581:intron 35.0%
ATAAAAAGTCTAGGCTGTGT+TGG + chr4.2:23958214-23958233 None:intergenic 35.0%
ATCAAACCCACAAGAAAAAC+AGG - chr4.2:23958589-23958608 MS.gene39581:intron 35.0%
ATCTATTGGAAATGTGAGTG+TGG - chr4.2:23959165-23959184 MS.gene39581:intron 35.0%
CATCAAAAGAGAGAGATCAA+TGG - chr4.2:23958161-23958180 MS.gene39581:CDS 35.0%
CTCGTTGTGGTTCTTATAAT+TGG - chr4.2:23956249-23956268 MS.gene39581:CDS 35.0%
GATTCAAACCATGTTCTTGA+GGG + chr4.2:23956331-23956350 None:intergenic 35.0%
GGGTCATTCAAATGGAATAA+AGG - chr4.2:23959073-23959092 MS.gene39581:intron 35.0%
GTCAAAATATTCAAGGCCAA+GGG - chr4.2:23958820-23958839 MS.gene39581:intron 35.0%
GTGTGAATTGGAAATTCTTC+AGG + chr4.2:23958616-23958635 None:intergenic 35.0%
TACCAGTCACTTGAATCTAT+TGG - chr4.2:23959151-23959170 MS.gene39581:intron 35.0%
TCAAAATATTCAAGGCCAAG+GGG - chr4.2:23958821-23958840 MS.gene39581:intron 35.0%
TCAAGTGACTGGTATTTATC+TGG + chr4.2:23959145-23959164 None:intergenic 35.0%
TCCATGTTATCAAAGGCAAA+AGG + chr4.2:23958698-23958717 None:intergenic 35.0%
TCGGAATAGACAATGTAAGA+AGG + chr4.2:23956192-23956211 None:intergenic 35.0%
TGATAGCTTACTGACAAAAG+AGG - chr4.2:23958101-23958120 MS.gene39581:CDS 35.0%
TGGTGTATCCATGTTATCAA+AGG + chr4.2:23958705-23958724 None:intergenic 35.0%
TGTAGCCAATTTGATGAGAA+TGG - chr4.2:23956480-23956499 MS.gene39581:CDS 35.0%
TTCCAATAGATTCAAGTGAC+TGG + chr4.2:23959156-23959175 None:intergenic 35.0%
TTCCTTTGTCACAAGGAATA+GGG - chr4.2:23959053-23959072 MS.gene39581:intron 35.0%
TTTCCTTTGTCACAAGGAAT+AGG - chr4.2:23959052-23959071 MS.gene39581:intron 35.0%
TTTGTATGTTGTTCTCGTTG+TGG - chr4.2:23956236-23956255 MS.gene39581:CDS 35.0%
! AAAGAGAAGAAGAAAGGTGT+AGG - chr4.2:23956990-23957009 MS.gene39581:intron 35.0%
! CTTGTCTTTTGTTCCGAATA+TGG + chr4.2:23958647-23958666 None:intergenic 35.0%
! GGTTTTTACACATCACTCTA+AGG - chr4.2:23956145-23956164 MS.gene39581:CDS 35.0%
! TCTCCTTGTTTCACAAGTTT+CGG - chr4.2:23958329-23958348 MS.gene39581:intron 35.0%
! TTTTGTTCCGAATATGGTCT+TGG + chr4.2:23958641-23958660 None:intergenic 35.0%
!! ATTTTGATACGGTACAAGCT+TGG + chr4.2:23956567-23956586 None:intergenic 35.0%
!! ATTTTTGTAAAGTCTGAGGC+AGG - chr4.2:23958060-23958079 MS.gene39581:CDS 35.0%
!! TTGATGAGAATGGACTGTAA+TGG - chr4.2:23956490-23956509 MS.gene39581:CDS 35.0%
!! TTGGTTTTCAGCAATCAAGA+GGG - chr4.2:23956124-23956143 MS.gene39581:CDS 35.0%
!! TTTCCTTGTGGAGTTCTAAA+TGG + chr4.2:23958785-23958804 None:intergenic 35.0%
!!! AAGAGTTTTAGAGCTCTGAA+AGG - chr4.2:23957683-23957702 MS.gene39581:intron 35.0%
!!! GATCTTGTTGATTTTGGAGT+TGG + chr4.2:23958725-23958744 None:intergenic 35.0%
!!! GTTCTAGATGGTTTTGAAGA+TGG + chr4.2:23958758-23958777 None:intergenic 35.0%
!!! TTTTAGAGCTCTGAAAGGTT+TGG - chr4.2:23957688-23957707 MS.gene39581:intron 35.0%
AAACGAAGTTCAGGCAAAAG+AGG + chr4.2:23956787-23956806 None:intergenic 40.0%
AAAGTCTCAGCTCAGAAAAC+AGG + chr4.2:23956401-23956420 None:intergenic 40.0%
ACACATATCCCTCAAGAACA+TGG - chr4.2:23956320-23956339 MS.gene39581:CDS 40.0%
AGACAATGTAAGAAGGCAGT+AGG + chr4.2:23956185-23956204 None:intergenic 40.0%
AGGCAGGTAACAATGAAGAT+TGG - chr4.2:23958076-23958095 MS.gene39581:CDS 40.0%
ATTACTACTAGCGCGTACTT+TGG + chr4.2:23958957-23958976 None:intergenic 40.0%
CGTCAAAATATTCAAGGCCA+AGG - chr4.2:23958819-23958838 MS.gene39581:intron 40.0%
GAATGTTCATCAACACAAGG+AGG - chr4.2:23957280-23957299 MS.gene39581:intron 40.0%
GCGTGACGTCAAAATATTCA+AGG - chr4.2:23958813-23958832 MS.gene39581:intron 40.0%
GGCAGGTAACAATGAAGATT+GGG - chr4.2:23958077-23958096 MS.gene39581:CDS 40.0%
GGCATTTGCATATTCACATC+AGG - chr4.2:23958182-23958201 MS.gene39581:CDS 40.0%
GGTGTAGGAAAACTAAAGCA+TGG - chr4.2:23957005-23957024 MS.gene39581:intron 40.0%
GTAAGGAGAACACCTCAAAA+AGG + chr4.2:23957430-23957449 None:intergenic 40.0%
GTACTTGCAATATATAGGCC+AGG - chr4.2:23957660-23957679 MS.gene39581:intron 40.0%
TAACAGCTGCATGTGTTTCA+TGG + chr4.2:23957768-23957787 None:intergenic 40.0%
TAAGAGGACAGAATGTGAAG+AGG - chr4.2:23957729-23957748 MS.gene39581:intron 40.0%
TCCAGCTTCATTCTTGTACT+CGG + chr4.2:23957858-23957877 None:intergenic 40.0%
TGCTTACAGAAACGAAGTTC+AGG + chr4.2:23956796-23956815 None:intergenic 40.0%
TTCCCGAAACTTGTGAAACA+AGG + chr4.2:23958335-23958354 None:intergenic 40.0%
TTTACAGAACCAGCATGTCT+CGG - chr4.2:23957325-23957344 MS.gene39581:intron 40.0%
! AAAGGTTTGGTAAGGCTTCA+AGG - chr4.2:23957701-23957720 MS.gene39581:intron 40.0%
! ATGTAGTTTGGAACGGAGAA+CGG + chr4.2:23958911-23958930 None:intergenic 40.0%
! CTCCTTGTTTCACAAGTTTC+GGG - chr4.2:23958330-23958349 MS.gene39581:intron 40.0%
! TACACATCACTCTAAGGGAA+AGG - chr4.2:23956151-23956170 MS.gene39581:CDS 40.0%
! TGGTTCTGTAAAGTTGGTTC+TGG + chr4.2:23957317-23957336 None:intergenic 40.0%
! TTTTCTTGTGGGTTTGATGC+AGG + chr4.2:23958587-23958606 None:intergenic 40.0%
!! AATGAAGCTGGAAGTACCTT+GGG - chr4.2:23957866-23957885 MS.gene39581:intron 40.0%
!! ACATGCTGGTTCTGTAAAGT+TGG + chr4.2:23957323-23957342 None:intergenic 40.0%
!! CTAAATGGAGGAGTTCTAGA+TGG + chr4.2:23958770-23958789 None:intergenic 40.0%
!! GCCTTTTGCCTTTGATAACA+TGG - chr4.2:23958694-23958713 MS.gene39581:intron 40.0%
!! TCAGGACCTGTTTTTCTTGT+GGG + chr4.2:23958598-23958617 None:intergenic 40.0%
!! TTCAGGACCTGTTTTTCTTG+TGG + chr4.2:23958599-23958618 None:intergenic 40.0%
!!! ACAGGCGTTTGAGTTGATTT+TGG + chr4.2:23958506-23958525 None:intergenic 40.0%
!!! CGTTTGAGTTGATTTTGGAG+TGG + chr4.2:23958501-23958520 None:intergenic 40.0%
!!! GTTGGTTTTCAGCAATCAAG+AGG - chr4.2:23956123-23956142 MS.gene39581:CDS 40.0%
AAAAGTCTAGGCTGTGTTGG+TGG + chr4.2:23958211-23958230 None:intergenic 45.0%
ACAGTTGGAGCCATGTAGTT+TGG + chr4.2:23958923-23958942 None:intergenic 45.0%
ACCTTTCGACGTACCATTGA+AGG - chr4.2:23958859-23958878 MS.gene39581:intron 45.0%
AGACTGTCATCGTCCTTCAA+TGG + chr4.2:23958875-23958894 None:intergenic 45.0%
CAAAAGACAAGTCCAAGACC+TGG - chr4.2:23958656-23958675 MS.gene39581:intron 45.0%
CAAAAGAGGAAGTAGAAGCG+AGG - chr4.2:23958115-23958134 MS.gene39581:CDS 45.0%
CACACCACCAAGACCATATT+CGG - chr4.2:23958631-23958650 MS.gene39581:intron 45.0%
CTCTTGGAATCAGTGCTTGT+TGG + chr4.2:23959022-23959041 None:intergenic 45.0%
GAAGAGGGGGATGAAAGAAT+TGG + chr4.2:23957036-23957055 None:intergenic 45.0%
GACCCTATTCCTTGTGACAA+AGG + chr4.2:23959058-23959077 None:intergenic 45.0%
GCGGAATGTTCATCAACACA+AGG - chr4.2:23957277-23957296 MS.gene39581:intron 45.0%
GGCTGCTTACAGAGGTTATA+TGG - chr4.2:23957361-23957380 MS.gene39581:intron 45.0%
GTCTCGTTTCCTTTGTCACA+AGG - chr4.2:23959046-23959065 MS.gene39581:intron 45.0%
GTTCTCCGTTCCAAACTACA+TGG - chr4.2:23958910-23958929 MS.gene39581:intron 45.0%
TATAACCTCTGTAAGCAGCC+TGG + chr4.2:23957361-23957380 None:intergenic 45.0%
TCCAATCACATTGAGCGTCT+CGG - chr4.2:23956422-23956441 MS.gene39581:CDS 45.0%
TCCTTCAATGGTACGTCGAA+AGG + chr4.2:23958863-23958882 None:intergenic 45.0%
TGGAGCCATGTAGTTTGGAA+CGG + chr4.2:23958918-23958937 None:intergenic 45.0%
TGGTACGTCGAAAGGTGAAT+TGG + chr4.2:23958855-23958874 None:intergenic 45.0%
TTTGGTAAGGCTTCAAGGAG+TGG - chr4.2:23957706-23957725 MS.gene39581:intron 45.0%
! AAAGTCTAGGCTGTGTTGGT+GGG + chr4.2:23958210-23958229 None:intergenic 45.0%
! ACGCTTGAAGTGTTTCCTTG+TGG + chr4.2:23958797-23958816 None:intergenic 45.0%
! CCTCCATTTAGAACTCCACA+AGG - chr4.2:23958779-23958798 MS.gene39581:intron 45.0%
! TAGGAGAAGCAACTCTAGGA+GGG + chr4.2:23957169-23957188 None:intergenic 45.0%
! TGTTCCGAATATGGTCTTGG+TGG + chr4.2:23958638-23958657 None:intergenic 45.0%
!! ACCTTGGGGAAAAGTGCTTT+AGG - chr4.2:23957881-23957900 MS.gene39581:intron 45.0%
!! ATGAAGCTGGAAGTACCTTG+GGG - chr4.2:23957867-23957886 MS.gene39581:intron 45.0%
!! ATGGTCTTGGTGGTGTGAAT+TGG + chr4.2:23958628-23958647 None:intergenic 45.0%
!! CAACAAGCACTGATTCCAAG+AGG - chr4.2:23959020-23959039 MS.gene39581:intron 45.0%
!! CAGAGCTCTAAAACTCTTCC+TGG + chr4.2:23957681-23957700 None:intergenic 45.0%
!! CCTTGTGGAGTTCTAAATGG+AGG + chr4.2:23958782-23958801 None:intergenic 45.0%
!! GAATGAAGCTGGAAGTACCT+TGG - chr4.2:23957865-23957884 MS.gene39581:intron 45.0%
!! GAGCTCTGAAAGGTTTGGTA+AGG - chr4.2:23957693-23957712 MS.gene39581:intron 45.0%
!! GGGAAAAGTGCTTTAGGACA+TGG - chr4.2:23957887-23957906 MS.gene39581:intron 45.0%
!! GTGCCTGGTTTTCTAACATC+TGG + chr4.2:23957826-23957845 None:intergenic 45.0%
!! TCCTAAAGCACTTTTCCCCA+AGG + chr4.2:23957885-23957904 None:intergenic 45.0%
!!! TATAAAAAATAAAAGTATTT+AGG - chr4.2:23957483-23957502 MS.gene39581:CDS 5.0%
AAGATCCAGGCTGCTTACAG+AGG - chr4.2:23957353-23957372 MS.gene39581:intron 50.0%
AAGTAGAAGCGAGGTTGCAG+AGG - chr4.2:23958124-23958143 MS.gene39581:CDS 50.0%
AGATGGGAAAGAAGGGGAGT+TGG - chr4.2:23956105-23956124 MS.gene39581:CDS 50.0%
AGATTTGGCGGTGGTAGTAG+TGG - chr4.2:23958986-23959005 MS.gene39581:intron 50.0%
AGCAGTATCCTTGCCTGCAA+GGG - chr4.2:23959192-23959211 MS.gene39581:CDS 50.0%
AGGAAACGAGACTCTCCTCT+TGG + chr4.2:23959038-23959057 None:intergenic 50.0%
AGGATTCTCATGGCTCTCAC+AGG - chr4.2:23959116-23959135 MS.gene39581:intron 50.0%
AGGCTTCAAGGAGTGGTAAG+AGG - chr4.2:23957713-23957732 MS.gene39581:intron 50.0%
CCAAGAGAAAGATTTGGCGG+TGG - chr4.2:23958977-23958996 MS.gene39581:intron 50.0%
CCACCGCCAAATCTTTCTCT+TGG + chr4.2:23958980-23958999 None:intergenic 50.0%
CTAGGAGAAGCAACTCTAGG+AGG + chr4.2:23957170-23957189 None:intergenic 50.0%
CTCCTAGAGTTGCTTCTCCT+AGG - chr4.2:23957168-23957187 MS.gene39581:CDS 50.0%
CTGCTGAGATGGGAAAGAAG+GGG - chr4.2:23956099-23956118 MS.gene39581:CDS 50.0%
GAATTGGATCCTACTCCCCT+TGG + chr4.2:23958839-23958858 None:intergenic 50.0%
GCCGAGTACAAGAATGAAGC+TGG - chr4.2:23957854-23957873 MS.gene39581:intron 50.0%
GCTGCTGAGATGGGAAAGAA+GGG - chr4.2:23956098-23956117 MS.gene39581:CDS 50.0%
GGCTTTCTTTCAACCCTTGC+AGG + chr4.2:23959208-23959227 None:intergenic 50.0%
TATTCAAGGCCAAGGGGAGT+AGG - chr4.2:23958827-23958846 MS.gene39581:intron 50.0%
TCCAAGGGATCCATCTCCAA+GGG - chr4.2:23957214-23957233 MS.gene39581:CDS 50.0%
TCCGAGACGCTCAATGTGAT+TGG + chr4.2:23956426-23956445 None:intergenic 50.0%
TCCTAAGCCTCCTTCTCCAA+GGG - chr4.2:23957199-23957218 MS.gene39581:CDS 50.0%
TCTTTCAACCCTTGCAGGCA+AGG + chr4.2:23959203-23959222 None:intergenic 50.0%
TGCTGCTGAGATGGGAAAGA+AGG - chr4.2:23956097-23956116 MS.gene39581:CDS 50.0%
! CTGCTTGGTTCTCTGAAGAG+GGG + chr4.2:23957050-23957069 None:intergenic 50.0%
! GCTGCTTGGTTCTCTGAAGA+GGG + chr4.2:23957051-23957070 None:intergenic 50.0%
! TAGTTTGGAACGGAGAACGG+TGG + chr4.2:23958908-23958927 None:intergenic 50.0%
! TGCTGCTTGGTTCTCTGAAG+AGG + chr4.2:23957052-23957071 None:intergenic 50.0%
! TGCTTGGTTCTCTGAAGAGG+GGG + chr4.2:23957049-23957068 None:intergenic 50.0%
!! TTGGCCTTGGCAGAAACAGT+TGG + chr4.2:23958938-23958957 None:intergenic 50.0%
ACTAGCGCGTACTTTGGCCT+TGG + chr4.2:23958951-23958970 None:intergenic 55.0%
AGAAGCGAGGTTGCAGAGGA+AGG - chr4.2:23958128-23958147 MS.gene39581:CDS 55.0%
AGAGGTAACTCGCGGAGAAG+CGG + chr4.2:23957240-23957259 None:intergenic 55.0%
CAGCAGTATCCTTGCCTGCA+AGG - chr4.2:23959191-23959210 MS.gene39581:CDS 55.0%
CCAACCAGTTCCACCACCAT+GGG + chr4.2:23958553-23958572 None:intergenic 55.0%
CTCCAAGGGATCCATCTCCA+AGG - chr4.2:23957213-23957232 MS.gene39581:CDS 55.0%
CTCCTAAGCCTCCTTCTCCA+AGG - chr4.2:23957198-23957217 MS.gene39581:CDS 55.0%
CTCGATCGCGTATCTGTCAC+AGG + chr4.2:23958524-23958543 None:intergenic 55.0%
GAAGGAGGCTTAGGAGAAGG+AGG + chr4.2:23957194-23957213 None:intergenic 55.0%
GACCTGGCTCAAGCTCTCAA+AGG - chr4.2:23958672-23958691 MS.gene39581:intron 55.0%
GATGGATCCCTTGGAGAAGG+AGG + chr4.2:23957209-23957228 None:intergenic 55.0%
GCGAGTTACCTCTCCGAAAG+CGG - chr4.2:23957247-23957266 MS.gene39581:CDS 55.0%
GCTTTCGGAGAGGTAACTCG+CGG + chr4.2:23957248-23957267 None:intergenic 55.0%
GGAGAAGGAGGCTTAGGAGA+AGG + chr4.2:23957197-23957216 None:intergenic 55.0%
GGAGATGGATCCCTTGGAGA+AGG + chr4.2:23957212-23957231 None:intergenic 55.0%
GGCCTAGGAGAAGCAACTCT+AGG + chr4.2:23957173-23957192 None:intergenic 55.0%
GTGACAGATACGCGATCGAG+CGG - chr4.2:23958524-23958543 MS.gene39581:intron 55.0%
TCCAACCAGTTCCACCACCA+TGG + chr4.2:23958554-23958573 None:intergenic 55.0%
TCCCTTGGAGAAGGAGGCTT+AGG + chr4.2:23957203-23957222 None:intergenic 55.0%
TCTTAGTCGCCGAGACATGC+TGG + chr4.2:23957337-23957356 None:intergenic 55.0%
TGAGAGCTTGAGCCAGGTCT+TGG + chr4.2:23958671-23958690 None:intergenic 55.0%
TGTCTCGGCGACTAAGATCC+AGG - chr4.2:23957340-23957359 MS.gene39581:intron 55.0%
! ACTCGGCTTGATATCGTGCC+TGG + chr4.2:23957841-23957860 None:intergenic 55.0%
! ACTCTAGGAGGGACGAAAGG+TGG + chr4.2:23957158-23957177 None:intergenic 55.0%
! GCAACTCTAGGAGGGACGAA+AGG + chr4.2:23957161-23957180 None:intergenic 55.0%
! GGGACGAAAGGTGGCGTTTT+TGG + chr4.2:23957149-23957168 None:intergenic 55.0%
!! GATTCCCATGGTGGTGGAAC+TGG - chr4.2:23958546-23958565 MS.gene39581:intron 55.0%
!! GGCTCCAACTGTTTCTGCCA+AGG - chr4.2:23958931-23958950 MS.gene39581:intron 55.0%
!! TTCCGCGAAGATGCCGCTTT+CGG + chr4.2:23957263-23957282 None:intergenic 55.0%
!!! AGGTGGCGTTTTTGGTCGCT+CGG + chr4.2:23957141-23957160 None:intergenic 55.0%
CTCCGAAAGCGGCATCTTCG+CGG - chr4.2:23957258-23957277 MS.gene39581:intron 60.0%
GCCCTTGGAGATGGATCCCT+TGG + chr4.2:23957218-23957237 None:intergenic 60.0%
GGCTTAGGAGAAGGAGGCCT+AGG + chr4.2:23957188-23957207 None:intergenic 60.0%
! GATCGAGCGGATTCCCATGG+TGG - chr4.2:23958537-23958556 MS.gene39581:intron 60.0%
! GGCCTTTGAGAGCTTGAGCC+AGG + chr4.2:23958677-23958696 None:intergenic 60.0%
! TTTGGTCGCTCGGGAGGTGT+GGG + chr4.2:23957131-23957150 None:intergenic 60.0%
! TTTTGGTCGCTCGGGAGGTG+TGG + chr4.2:23957132-23957151 None:intergenic 60.0%
!! CCCATGGTGGTGGAACTGGT+TGG - chr4.2:23958550-23958569 MS.gene39581:intron 60.0%
!! CGAAGATGCCGCTTTCGGAG+AGG + chr4.2:23957258-23957277 None:intergenic 60.0%
!!! GGTGGCGTTTTTGGTCGCTC+GGG + chr4.2:23957140-23957159 None:intergenic 60.0%
GGAGAAGCGGCCCTTGGAGA+TGG + chr4.2:23957227-23957246 None:intergenic 65.0%
! CGAGCGGATTCCCATGGTGG+TGG - chr4.2:23958540-23958559 MS.gene39581:intron 65.0%
! CGCGATCGAGCGGATTCCCA+TGG - chr4.2:23958534-23958553 MS.gene39581:intron 65.0%
!!! GGCGTTTTTGGTCGCTCGGG+AGG + chr4.2:23957137-23957156 None:intergenic 65.0%
ACTCGCGGAGAAGCGGCCCT+TGG + chr4.2:23957233-23957252 None:intergenic 70.0%
! GGTCGCTCGGGAGGTGTGGG+AGG + chr4.2:23957128-23957147 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 23956096 23959246 23956096 ID=MS.gene39581
chr4.2 mRNA 23956096 23959246 23956096 ID=MS.gene39581.t1;Parent=MS.gene39581
chr4.2 exon 23959170 23959246 23959170 ID=MS.gene39581.t1.exon1;Parent=MS.gene39581.t1
chr4.2 CDS 23959170 23959246 23959170 ID=cds.MS.gene39581.t1;Parent=MS.gene39581.t1
chr4.2 exon 23957960 23958284 23957960 ID=MS.gene39581.t1.exon2;Parent=MS.gene39581.t1
chr4.2 CDS 23957960 23958284 23957960 ID=cds.MS.gene39581.t1;Parent=MS.gene39581.t1
chr4.2 exon 23957432 23957665 23957432 ID=MS.gene39581.t1.exon3;Parent=MS.gene39581.t1
chr4.2 CDS 23957432 23957665 23957432 ID=cds.MS.gene39581.t1;Parent=MS.gene39581.t1
chr4.2 exon 23957139 23957270 23957139 ID=MS.gene39581.t1.exon4;Parent=MS.gene39581.t1
chr4.2 CDS 23957139 23957270 23957139 ID=cds.MS.gene39581.t1;Parent=MS.gene39581.t1
chr4.2 exon 23956096 23956854 23956096 ID=MS.gene39581.t1.exon5;Parent=MS.gene39581.t1
chr4.2 CDS 23956096 23956854 23956096 ID=cds.MS.gene39581.t1;Parent=MS.gene39581.t1
Gene Sequence

>MS.gene39581

ATGCTGCTGAGATGGGAAAGAAGGGGAGTTGGTTTTCAGCAATCAAGAGGGTTTTTACACATCACTCTAAGGGAAAGGTAATTTATCCTACTGCCTTCTTACATTGTCTATTCCGAGAAATGTGAAATGCTTAATTTGTTTTTGTATGTTGTTCTCGTTGTGGTTCTTATAATTGGTTTTCTTTTGGCATTAGATGCCAAAATTGTTAACCATTTAAGGATATGACACATATCCCTCAAGAACATGGTTTGAATCTCATCACTACTGATGTGAAGATTTCATGATAGAGTAGTTTGTAAGTACCTGTTTTCTGAGCTGAGACTTTTTCCAATCACATTGAGCGTCTCGGATTCAACTCACCTGAACTGTTATTGACAAAAAAGTTGTAGCCAATTTGATGAGAATGGACTGTAATGGATAGACCTTTGATAATATTAAGAAAATTCTTCCTTATGAGTTATGAACATTCCAAGCTTGTACCGTATCAAAATTTCGGTTGATTGGCCTTTTAATTCATATCAAGGTCCTGCATCTTAATTTTTTTTGTCAAATGTCAATTAGTTTTGTTCGTAACTACTAATAGATTATTTGAAATATTGCAAAAGGATTTAGGATATTGCTTTTAATTCTTCCCATCATTCTTATCTTACATCATATTTGCTTTATGCATTAGTTTTAAATTATTTTACCTCTTTTGCCTGAACTTCGTTTCTGTAAGCAACATATGCATGATGATATCATTATTTTACTACGAATCTGGTGTTTTCGTTATTCTGCCTTTGAAGTTGTTTTAAGTTATGTTACTGAATTTGAACTTGTTTCAGTTCTTCATATTGATCTATCCTAGTCTAATCATGCTTATTAACATAGCAGGATTCAGAAAACAAAAGCACAAAAGAGAAGAAGAAAGGTGTAGGAAAACTAAAGCATGGAGAGACCAATTCTTTCATCCCCCTCTTCAGAGAACCAAGCAGCATTGAGAAAATATTTGGGGACTTTGAAAGAGAACAACAAGTACTTGCAATTAGGCCTCCCACACCTCCCGAGCGACCAAAAACGCCACCTTTCGTCCCTCCTAGAGTTGCTTCTCCTAGGCCTCCTTCTCCTAAGCCTCCTTCTCCAAGGGATCCATCTCCAAGGGCCGCTTCTCCGCGAGTTACCTCTCCGAAAGCGGCATCTTCGCGGAATGTTCATCAACACAAGGAGGTTCGCAACAGACCAGAACCAACTTTACAGAACCAGCATGTCTCGGCGACTAAGATCCAGGCTGCTTACAGAGGTTATATGGTAAGCTTTTATATTTTATTGTTAATGTAATTAATAGAATTTAGCCTTTTTGAGGTGTTCTCCTTACAAACATATTCGGGAATTAAAATAGAAACTATAATATAAAAAATAAAAGTATTTAGGTTAAGTTTTGATTAACTTTTAGTATATGAGAGTACCTCATCTAAGATAAGTATTAAGTATTTGGTTTCTAAATTTGGAAAATGCCGCTTGATCCATTAATTTTACCAGCTTTTTTATCGATCATGAAACAAAAGTATCCTAACTCTTATATGTGGTACTTGCAATATATAGGCCAGGAAGAGTTTTAGAGCTCTGAAAGGTTTGGTAAGGCTTCAAGGAGTGGTAAGAGGACAGAATGTGAAGAGGCAGACAGTTAATGCCATGAAACACATGCAGCTGTTAGTGCGAGTTCAGTCTCAGATTCAATCTCGAAGAATCCAGATGTTAGAAAACCAGGCACGATATCAAGCCGAGTACAAGAATGAAGCTGGAAGTACCTTGGGGAAAAGTGCTTTAGGACATGGTGTAAGTTATCATACTCTTCTTTATCGTATCCATCAATTTTATATAAAGTAACTTCTTAATATCTAATACTTCTATTCTGAAATATGTGCATGATTATATTTTCAACTGCCTTTATATAAATCAGACTAATTTATTTGTGATAATTTGGAATTTTTGTAAAGTCTGAGGCAGGTAACAATGAAGATTGGGATGATAGCTTACTGACAAAAGAGGAAGTAGAAGCGAGGTTGCAGAGGAAGGTTGAAGCAATCATCAAAAGAGAGAGATCAATGGCATTTGCATATTCACATCAGGTACCCACCAACACAGCCTAGACTTTTTATTTATGCTTTTATTTTGGCTTAAATACGCTTATTATCCACCTATTTTAATCGAAACAAAAATTTCAGTACACCCATTTCTAAATCTAAGATTTTTGTCTCCTTGTTTCACAAGTTTCGGGAACTTTTTCCACAATCCAGTTTAGCTAATTTTTTATGATGTAGCATATTCATTAATGATGTGGAATTATATTTTATGATGCGACGTTGCTAATTTAGATCACTATTTGTTATGACTTTGTTGACTATGTTAACAGTTATGGAAGGCCACTCCAAAATCAACTCAAACGCCTGTGACAGATACGCGATCGAGCGGATTCCCATGGTGGTGGAACTGGTTGGAAAGACAATTACCTGCATCAAACCCACAAGAAAAACAGGTCCTGAAGAATTTCCAATTCACACCACCAAGACCATATTCGGAACAAAAGACAAGTCCAAGACCTGGCTCAAGCTCTCAAAGGCCTTTTGCCTTTGATAACATGGATACACCAACTCCAAAATCAACAAGATCGACTATATTTCCATCTTCAAAACCATCTAGAACTCCTCCATTTAGAACTCCACAAGGAAACACTTCAAGCGTGACGTCAAAATATTCAAGGCCAAGGGGAGTAGGATCCAATTCACCTTTCGACGTACCATTGAAGGACGATGACAGTCTCACAAGCTGTCCACCGTTCTCCGTTCCAAACTACATGGCTCCAACTGTTTCTGCCAAGGCCAAAGTACGCGCTAGTAGTAATCCAAGAGAAAGATTTGGCGGTGGTAGTAGTGGCTGTGCAACTCCAACAAGCACTGATTCCAAGAGGAGAGTCTCGTTTCCTTTGTCACAAGGAATAGGGTCATTCAAATGGAATAAAGGGTCAATGTTCTCTAGAAATAAGGATTCTCATGGCTCTCACAGGACTCCAGATAAATACCAGTCACTTGAATCTATTGGAAATGTGAGTGTGGATTCAGCAGTATCCTTGCCTGCAAGGGTTGAAAGAAAGCCTTTTACAAGATTTGTGTGA
Protein sequence

>MS.gene39581.t1

MLLRWERRGVGFQQSRGFLHITLREREPSSIEKIFGDFEREQQVLAIRPPTPPERPKTPPFVPPRVASPRPPSPKPPSPRDPSPRAASPRVTSPKAASSRNVHQHKEVRNRPEPTLQNQHVSATKIQAAYRGYMARKSFRALKGLVRLQGVVRGQNVKRQTVNAMKHMQLLVRVQSQIQSRRIQMLENQARYQAEYKNEAGSTLGKSALGHGSEAGNNEDWDDSLLTKEEVEARLQRKVEAIIKRERSMAFAYSHQLWKATPKSTQTPVTDTRSSGFPWWWNWLERQLPASNPQEKQVLKNFQFTPPRPYSEQKTSPRPGSSSQRPFAFDNMDTPTPKSTRSTIFPSSKPSRTPPFRTPQGNTSSVTSKYSRPRGVGSNSPFDVPLKDDDSLTSCPPFSVPNYMAPTVSAKAKVRASSNPRERFGGGSSGCATPTSTDSKRRVSFPLSQGIGSFKWNKGSMFSRNKDSHGSHRTPDKYQSLESIGNVSVDSAVSLPARVERKPFTRFV