AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39631

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39631.t1 MTR_4g086420 99.024 410 4 0 288 697 1 410 0.0 839
MS.gene39631.t1 MTR_4g086440 77.215 316 23 2 1 272 9 319 5.44e-169 487
MS.gene39631.t1 MTR_7g047570 61.682 321 72 2 1 270 10 330 1.83e-132 394
MS.gene39631.t1 MTR_7g451770 61.682 321 72 2 1 270 10 330 1.83e-132 394
MS.gene39631.t1 MTR_7g451800 61.059 321 74 2 1 270 10 330 7.85e-131 390
MS.gene39631.t1 MTR_7g047620 61.059 321 74 2 1 270 10 330 7.85e-131 390
MS.gene39631.t1 MTR_3g101490 48.768 406 198 6 298 697 5 406 3.96e-126 380
MS.gene39631.t1 MTR_1g092460 51.818 220 102 2 2 220 153 369 1.37e-72 242
MS.gene39631.t1 MTR_0274s0030 50.667 225 107 2 2 225 90 311 2.16e-72 239
MS.gene39631.t1 MTR_2g079270 52.655 226 101 3 2 225 283 504 1.48e-71 245
MS.gene39631.t1 MTR_4g088835 52.018 223 105 2 7 228 651 872 9.16e-69 242
MS.gene39631.t1 MTR_1g112730 50.679 221 103 2 1 220 833 1048 1.03e-67 241
MS.gene39631.t1 MTR_2g013650 52.093 215 101 2 7 220 661 874 2.16e-66 236
MS.gene39631.t1 MTR_4g020620 47.984 248 105 4 1 247 610 834 1.02e-65 233
MS.gene39631.t1 MTR_8g060700 59.091 198 80 1 29 225 502 699 5.98e-65 229
MS.gene39631.t1 MTR_1g092465 48.649 222 102 3 20 240 233 443 3.34e-62 215
MS.gene39631.t1 MTR_2g018540 53.093 194 89 1 29 220 108 301 5.88e-62 211
MS.gene39631.t1 MTR_7g023760 47.511 221 97 3 2 220 67 270 5.85e-61 207
MS.gene39631.t1 MTR_1g092440 53.889 180 82 1 42 220 394 573 1.27e-59 212
MS.gene39631.t1 MTR_4g020620 50.515 194 86 2 1 193 552 736 2.58e-56 205
MS.gene39631.t1 MTR_0332s0040 52.809 178 83 1 44 220 172 349 3.06e-53 189
MS.gene39631.t1 MTR_8g060700 60.843 166 64 1 29 193 502 667 3.22e-53 195
MS.gene39631.t1 MTR_8g060700 60.843 166 64 1 29 193 502 667 4.36e-53 194
MS.gene39631.t1 MTR_4g007210 36.741 313 157 9 2 306 57 336 1.70e-45 167
MS.gene39631.t1 MTR_1g092490 50.000 146 72 1 76 220 58 203 7.69e-43 157
MS.gene39631.t1 MTR_2g079280 40.541 222 96 5 2 220 100 288 7.61e-42 154
MS.gene39631.t1 MTR_8g468530 33.784 148 85 5 29 176 23 157 1.86e-16 77.8
MS.gene39631.t1 MTR_4g108080 33.784 148 85 5 29 176 7 141 4.71e-16 76.3
MS.gene39631.t1 MTR_4g108080 39.130 92 53 2 29 119 7 96 4.02e-15 72.4
MS.gene39631.t1 MTR_3g062450 32.812 128 74 4 29 156 4 119 6.14e-15 73.2
MS.gene39631.t1 MTR_5g075580 32.812 128 74 4 29 156 4 119 6.26e-15 72.8
MS.gene39631.t1 MTR_7g116940 31.343 134 75 4 29 156 4 126 3.61e-14 70.9
MS.gene39631.t1 MTR_7g116940 32.031 128 75 4 29 156 4 119 4.19e-14 70.5
MS.gene39631.t1 MTR_1g021150 32.812 128 74 4 29 156 4 119 5.60e-14 70.1
MS.gene39631.t1 MTR_8g085690 33.333 126 74 3 25 150 21 136 8.51e-14 70.5
MS.gene39631.t1 MTR_7g116940 33.058 121 70 3 29 147 4 115 3.03e-13 67.4
MS.gene39631.t1 MTR_8g071040 31.250 128 76 4 29 156 4 119 4.05e-13 67.8
MS.gene39631.t1 MTR_4g073240 32.031 128 75 4 29 156 4 119 4.68e-13 67.8
MS.gene39631.t1 MTR_5g075580 33.673 98 53 4 59 156 5 90 1.00e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39631.t1 AT2G15695 54.374 423 168 7 296 697 2 420 3.52e-151 445
MS.gene39631.t1 AT5G44250 46.305 406 205 8 298 697 5 403 3.88e-116 355
MS.gene39631.t1 AT5G44250 45.955 309 156 7 393 697 1 302 1.09e-82 264
MS.gene39631.t1 AT1G53025 49.593 246 118 3 2 246 250 490 1.53e-69 237
MS.gene39631.t1 AT1G53025 49.593 246 118 3 2 246 250 490 1.94e-69 237
MS.gene39631.t1 AT2G16920 56.186 194 84 1 28 220 852 1045 2.77e-68 243
MS.gene39631.t1 AT2G16920 56.186 194 84 1 28 220 852 1045 2.77e-68 243
MS.gene39631.t1 AT1G53023 45.091 275 128 6 2 264 27 290 2.99e-67 224
MS.gene39631.t1 AT3G15355 51.584 221 103 2 1 220 310 527 6.65e-66 229
MS.gene39631.t1 AT2G33770 52.941 221 102 2 1 220 638 857 3.00e-62 224
MS.gene39631.t1 AT2G02760 33.784 148 85 5 29 176 7 141 6.95e-17 79.0
MS.gene39631.t1 AT2G02760 33.784 148 85 5 29 176 7 141 6.95e-17 79.0
MS.gene39631.t1 AT2G02760 33.784 148 85 5 29 176 7 141 6.95e-17 79.0
MS.gene39631.t1 AT2G02760 33.784 148 85 5 29 176 7 141 6.95e-17 79.0
MS.gene39631.t1 AT1G14400 33.108 148 86 5 29 176 7 141 1.88e-16 77.4
MS.gene39631.t1 AT1G14400 33.108 148 86 5 29 176 7 141 1.88e-16 77.4
MS.gene39631.t1 AT5G41700 32.812 128 74 4 29 156 4 119 8.42e-15 72.8
MS.gene39631.t1 AT5G41700 32.812 128 74 4 29 156 4 119 8.42e-15 72.8
MS.gene39631.t1 AT5G41700 32.812 128 74 4 29 156 4 119 8.42e-15 72.8
MS.gene39631.t1 AT5G41700 33.333 129 73 5 29 156 4 120 1.26e-14 72.0
MS.gene39631.t1 AT4G27960 32.031 128 75 4 29 156 4 119 2.01e-14 71.6
MS.gene39631.t1 AT1G64230 32.031 128 75 4 29 156 4 119 2.90e-14 71.2
MS.gene39631.t1 AT1G64230 32.031 128 75 4 29 156 4 119 2.90e-14 71.2
MS.gene39631.t1 AT5G53300 32.031 128 75 4 29 156 4 119 3.76e-14 70.9
MS.gene39631.t1 AT5G53300 32.031 128 75 4 29 156 4 119 3.76e-14 70.9
MS.gene39631.t1 AT5G53300 32.031 128 75 4 29 156 4 119 3.76e-14 70.9
MS.gene39631.t1 AT3G08690 32.031 128 75 4 29 156 4 119 4.93e-14 70.5
MS.gene39631.t1 AT3G08690 32.031 128 75 4 29 156 4 119 4.93e-14 70.5
MS.gene39631.t1 AT5G41700 29.371 143 83 4 29 171 4 128 1.86e-13 68.6
MS.gene39631.t1 AT4G27960 32.031 128 75 4 29 156 34 149 3.22e-13 68.9
MS.gene39631.t1 AT5G56150 32.031 128 75 4 29 156 4 119 1.40e-12 66.2
MS.gene39631.t1 AT5G53300 33.333 102 59 2 29 130 4 96 1.99e-12 64.7
MS.gene39631.t1 AT1G64230 32.117 137 72 7 29 156 4 128 8.36e-12 64.3
MS.gene39631.t1 AT5G62540 30.872 149 90 5 29 177 7 142 1.67e-11 63.2
MS.gene39631.t1 AT5G56150 33.333 102 59 2 29 130 4 96 2.15e-11 61.6
MS.gene39631.t1 AT2G16740 31.538 130 73 6 29 156 4 119 2.56e-11 62.8
MS.gene39631.t1 AT3G57870 35.433 127 67 6 30 150 9 126 9.76e-11 61.2

Find 146 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGTTGTCTTCAGAATTT+TGG 0.059999 4.2:+24531585 MS.gene39631:intron
CTAGATGTTACAAGTGATTT+TGG 0.148183 4.2:+24531318 MS.gene39631:CDS
TTGTTTAGGAACTGTGTTTC+TGG 0.150597 4.2:+24531273 MS.gene39631:CDS
AGCTGCTGTGAGCCATTTAT+TGG 0.192871 4.2:+24531862 MS.gene39631:CDS
AATTCACGTGTTTCGCTTAC+TGG 0.198836 4.2:+24528732 MS.gene39631:CDS
CTGTCTGTACAAATTATTTC+AGG 0.229050 4.2:+24530996 MS.gene39631:CDS
TCCCAACCTTCAACATTCTT+TGG 0.240471 4.2:-24532202 None:intergenic
ATGCGTTTAATTCCTCTAAA+AGG 0.247530 4.2:-24532271 None:intergenic
AGATTCATTATCACTCTTTC+GGG 0.251709 4.2:+24524297 MS.gene39631:CDS
GTTGACCTTTACTCCTCTTT+AGG 0.255986 4.2:+24528907 MS.gene39631:CDS
ACAAATTCCTCAAAGTTCTT+TGG 0.257881 4.2:-24524599 None:intergenic
AAGATTCATTATCACTCTTT+CGG 0.270164 4.2:+24524296 MS.gene39631:CDS
TCTGGACATATATATGATTC+TGG 0.283472 4.2:+24531291 MS.gene39631:CDS
CGCTGTGAGCGCTGATGCTT+GGG 0.291912 4.2:-24532147 None:intergenic
TGGTGGATTGTTTGGGTAAT+CGG 0.299445 4.2:-24524273 None:intergenic
AACAACAATGATAGAGAATC+TGG 0.310572 4.2:+24532076 MS.gene39631:CDS
AGTTCCACAAAACCTGTTAC+AGG 0.311517 4.2:+24528879 MS.gene39631:CDS
GAAAGGAACGATTGAATATA+CGG 0.313704 4.2:-24524496 None:intergenic
GACCATCAAAGCTTGTTTCT+TGG 0.325748 4.2:+24531382 MS.gene39631:CDS
TTCATAGGCTTTGACGTAGA+TGG 0.327076 4.2:-24524159 None:intergenic
GGCAATGATCAAACAAGCTT+TGG 0.332974 4.2:+24524946 MS.gene39631:CDS
TGAATCTTTGGTGGATTGTT+TGG 0.335822 4.2:-24524281 None:intergenic
GGTCAACGAATTCCTGTAAC+AGG 0.339829 4.2:-24528891 None:intergenic
TAAATCGATGTGGGTTTCAT+AGG 0.344657 4.2:-24524174 None:intergenic
TGCCGTCAGGGTAAAGATTA+GGG 0.352393 4.2:-24524331 None:intergenic
ACACGGCCTTCTCCAATAAA+TGG 0.355167 4.2:-24531874 None:intergenic
AAAGAGTGATAATGAATCTT+TGG 0.365671 4.2:-24524293 None:intergenic
AGCATGACTGTCAATTGTTT+AGG 0.377794 4.2:+24531259 MS.gene39631:intron
GAACTTTGAGGAATTTGTTA+AGG 0.380913 4.2:+24524604 MS.gene39631:CDS
GCCGTTATCGTCGGCGCAGC+CGG 0.382156 4.2:+24524203 MS.gene39631:CDS
ATCAGCGCTCACAGCGTCCT+TGG 0.388256 4.2:+24532154 MS.gene39631:CDS
GTGCCGTCAGGGTAAAGATT+AGG 0.393143 4.2:-24524332 None:intergenic
ACCATCAAAGCTTGTTTCTT+GGG 0.398334 4.2:+24531383 MS.gene39631:CDS
TCCGAACATAAAATGAGAAA+TGG 0.399424 4.2:-24531612 None:intergenic
GTAGCAAAGTCTGTTGCATC+TGG 0.399698 4.2:+24531405 MS.gene39631:CDS
ACCCAAGAAACAAGCTTTGA+TGG 0.403500 4.2:-24531384 None:intergenic
GGCTCACAGCAGCTCTGTAT+TGG 0.405682 4.2:-24531853 None:intergenic
AATCTCAATCAGCTGATCAT+TGG 0.409867 4.2:+24531457 MS.gene39631:CDS
TGAAGAAGAGTAGACCTAGA+TGG 0.413531 4.2:-24524410 None:intergenic
ACCTTTACTCCTCTTTAGGT+TGG 0.415566 4.2:+24528911 MS.gene39631:CDS
CGAGAAAGAACTGGTATTGA+TGG 0.422478 4.2:+24531926 MS.gene39631:CDS
GAATCGTTGTAATCTTTGCT+TGG 0.425804 4.2:+24528851 MS.gene39631:CDS
AGAAACAAGCTTTGATGGTC+CGG 0.428964 4.2:-24531379 None:intergenic
AATCGTTGTAATCTTTGCTT+GGG 0.431280 4.2:+24528852 MS.gene39631:CDS
CTTAAGCAAACAGTGCCGTC+AGG 0.434766 4.2:-24524344 None:intergenic
ACGCTGTGAGCGCTGATGCT+TGG 0.436180 4.2:-24532148 None:intergenic
GAATCTTTGGTGGATTGTTT+GGG 0.438171 4.2:-24524280 None:intergenic
TCAGCGCTCACAGCGTCCTT+GGG 0.444207 4.2:+24532155 MS.gene39631:CDS
GGCTTTGACGTAGATGGAAT+CGG 0.445322 4.2:-24524153 None:intergenic
CTGAAATAATTTGTACAGAC+AGG 0.451620 4.2:-24530995 None:intergenic
GCAATGATCAAACAAGCTTT+GGG 0.451825 4.2:+24524947 MS.gene39631:CDS
ACACTGCATATTTGATCATT+CGG 0.454865 4.2:+24524570 MS.gene39631:CDS
TACGAGGCGGTAACATCTAC+TGG 0.455529 4.2:+24528800 MS.gene39631:CDS
ATCGCTATGCGTGGAGTTGA+AGG 0.456928 4.2:+24528769 MS.gene39631:CDS
AACACATGGCCTAGTACAAA+GGG 0.457576 4.2:+24524374 MS.gene39631:CDS
GGGAGAAGTGGGACCCATCT+AGG 0.464511 4.2:+24524396 MS.gene39631:CDS
TGAGGAAAGTGCGGTGCCAT+TGG 0.467523 4.2:+24530600 MS.gene39631:CDS
AATTAATTGCAACTTTCTGC+AGG 0.468910 4.2:-24531982 None:intergenic
AATTCCTCAAAGTTCTTTGG+CGG 0.472954 4.2:-24524596 None:intergenic
TGGGAAGCTAGGAGCACAAA+CGG 0.476633 4.2:-24532128 None:intergenic
TTGTTATGTCCTGCTGAACT+AGG 0.479809 4.2:-24532058 None:intergenic
GCTTTGACGTAGATGGAATC+GGG 0.479816 4.2:-24524152 None:intergenic
TCCAAAGAATGTTGAAGGTT+GGG 0.486461 4.2:+24532201 MS.gene39631:CDS
AAGAGAATTCCAACCTAAAG+AGG 0.487518 4.2:-24528920 None:intergenic
AAAGTCTGTTGCATCTGGTT+TGG 0.487705 4.2:+24531410 MS.gene39631:CDS
GAAGATTCCATTCCCAGCAT+TGG 0.493338 4.2:-24524912 None:intergenic
AAGAGCATGAAATCCAATGC+TGG 0.494412 4.2:+24524899 MS.gene39631:CDS
CAACACATGGCCTAGTACAA+AGG 0.503183 4.2:+24524373 MS.gene39631:CDS
CTTCAAACTCCAAGTCATGA+TGG 0.503317 4.2:-24524868 None:intergenic
ACCGGCTGCGCCGACGATAA+CGG 0.504558 4.2:-24524204 None:intergenic
TACTAGGCCATGTGTTGAGA+AGG 0.504919 4.2:-24524367 None:intergenic
TGTGTTCCAAAGAATGTTGA+AGG 0.505151 4.2:+24532196 MS.gene39631:CDS
TCTTCTTTCTGTTCGTCCCG+TGG 0.505476 4.2:-24532103 None:intergenic
TTTAGAGGAATTAAACGCAT+TGG 0.506775 4.2:+24532274 MS.gene39631:CDS
ATTCACGTGTTTCGCTTACT+GGG 0.507630 4.2:+24528733 MS.gene39631:CDS
CGTGTAAAGAGTACGTGAAT+GGG 0.509143 4.2:+24524663 MS.gene39631:CDS
TCCAACCTAAAGAGGAGTAA+AGG 0.510951 4.2:-24528912 None:intergenic
CTTTCATGATTCGTTTGTAG+AGG 0.511625 4.2:-24524103 None:intergenic
GAGTGATAATGAATCTTTGG+TGG 0.513858 4.2:-24524290 None:intergenic
ACAGCAGCTCTGTATTGGAT+TGG 0.516355 4.2:-24531848 None:intergenic
ATACTTGAACGAGAAAGAAC+TGG 0.518031 4.2:+24531917 MS.gene39631:CDS
AACCCTAATCTTTACCCTGA+CGG 0.518674 4.2:+24524329 MS.gene39631:CDS
TAAGGAGTGTGGTGCTTCGT+TGG 0.521046 4.2:+24524838 MS.gene39631:CDS
AAGAACAGACCGTCGTGGTA+AGG 0.521914 4.2:-24524230 None:intergenic
GAAACAAGCTTTGATGGTCC+GGG 0.522040 4.2:-24531378 None:intergenic
GAAGAAGAGTAGACCTAGAT+GGG 0.524535 4.2:-24524409 None:intergenic
AAATCACTTGTAACATCTAG+TGG 0.525521 4.2:-24531315 None:intergenic
ATGCACAGCTCCACTCAACT+TGG 0.526381 4.2:+24531142 MS.gene39631:CDS
TCAATCAGCTGATCATTGGC+AGG 0.527299 4.2:+24531461 MS.gene39631:CDS
TATACAAAGTTTAAGGAGTG+TGG 0.527907 4.2:+24524827 MS.gene39631:intron
GGATTGTTTGGGTAATCGGC+AGG 0.529908 4.2:-24524269 None:intergenic
TTTGGGTTACGCAGGTGCTA+AGG 0.530778 4.2:+24530923 MS.gene39631:intron
GCGCTGATGCTTGGGAAGCT+AGG 0.531134 4.2:-24532139 None:intergenic
ATGAAATCCAATGCTGGGAA+TGG 0.531682 4.2:+24524905 MS.gene39631:CDS
AAGTGACTTGGCTTCATGAA+AGG 0.534999 4.2:-24524513 None:intergenic
TTACGCAGGTGCTAAGGACT+AGG 0.538684 4.2:+24530929 MS.gene39631:intron
CTCTTCATCCAGGTTCAATG+AGG 0.542042 4.2:+24530582 MS.gene39631:intron
TTTCATGATTCGTTTGTAGA+GGG 0.543653 4.2:-24524102 None:intergenic
ACCGTCGTGGTAAGGTGTAC+CGG 0.543663 4.2:-24524222 None:intergenic
GCGTGTAAAGAGTACGTGAA+TGG 0.547886 4.2:+24524662 MS.gene39631:CDS
TTACTCCGCGCCGTTATCGT+CGG 0.548228 4.2:+24524194 MS.gene39631:CDS
CCTTCCATTGCAAAAGTGCC+CGG 0.550129 4.2:+24531360 MS.gene39631:CDS
GACACCGCCAAAGAACTTTG+AGG 0.554472 4.2:+24524592 MS.gene39631:CDS
TTCCAAAGAATGTTGAAGGT+TGG 0.557791 4.2:+24532200 MS.gene39631:CDS
GGAGACTTTCCATCATGACT+TGG 0.559503 4.2:+24524859 MS.gene39631:CDS
TGTGAGCCATTTATTGGAGA+AGG 0.562192 4.2:+24531868 MS.gene39631:CDS
GCTTAAGCCTTCTCAACACA+TGG 0.566961 4.2:+24524360 MS.gene39631:CDS
TTCATTGTAAGTAAGTGACT+TGG 0.568715 4.2:-24524525 None:intergenic
AATTGCATTACCAAGTTGAG+TGG 0.572964 4.2:-24531152 None:intergenic
CGATTGAATATACGGTAAAG+AGG 0.575910 4.2:-24524488 None:intergenic
GGGAAGCTAGGAGCACAAAC+GGG 0.575953 4.2:-24532127 None:intergenic
AGAGCATGAAATCCAATGCT+GGG 0.578202 4.2:+24524900 MS.gene39631:CDS
TGATCAAACAAGCTTTGGGA+TGG 0.587381 4.2:+24524951 MS.gene39631:CDS
ATAGAGAATCTGGGATTCCA+CGG 0.590079 4.2:+24532086 MS.gene39631:CDS
TTAAACGCATTGGTCGCTCT+AGG 0.593892 4.2:+24532284 MS.gene39631:CDS
GAAGCAAACACAACAGGACA+TGG 0.602944 4.2:-24530952 None:intergenic
ATACAAATGCAGCTTAGTGA+AGG 0.607255 4.2:+24531116 MS.gene39631:intron
TTAAGCAAACAGTGCCGTCA+GGG 0.607850 4.2:-24524343 None:intergenic
ACGAGGCGGTAACATCTACT+GGG 0.608927 4.2:+24528801 MS.gene39631:CDS
AAGGAACACTTTCGTGAACG+AGG 0.609953 4.2:+24524623 MS.gene39631:CDS
AAACACGACACTGATTTCAA+AGG 0.610001 4.2:+24528829 MS.gene39631:CDS
ACAACAATGATAGAGAATCT+GGG 0.610595 4.2:+24532077 MS.gene39631:CDS
GTTGAAGGTTGTGCCGTACG+AGG 0.611614 4.2:+24528784 MS.gene39631:CDS
TGGAATCTTCAACAATGCAA+TGG 0.614688 4.2:+24524925 MS.gene39631:CDS
TCTCATATCATCGCTATGCG+TGG 0.615765 4.2:+24528760 MS.gene39631:CDS
TTCCACAGTGACAACATCAG+TGG 0.619814 4.2:+24524053 MS.gene39631:CDS
ACAAGCTTTGGGATGGACGA+AGG 0.621993 4.2:+24524958 MS.gene39631:CDS
GTAGATGTTACCGCCTCGTA+CGG 0.628127 4.2:-24528797 None:intergenic
GGCGCGGAGTAAATCGATGT+GGG 0.629814 4.2:-24524183 None:intergenic
TTTGTGGGAATCGGAATGGG+AGG 0.631152 4.2:+24532236 MS.gene39631:CDS
TAGAGAATCTGGGATTCCAC+GGG 0.635772 4.2:+24532087 MS.gene39631:CDS
ACAAACGAATCATGAAAGAG+TGG 0.637712 4.2:+24524108 MS.gene39631:CDS
CGGCGCGGAGTAAATCGATG+TGG 0.647111 4.2:-24524184 None:intergenic
AGGAACACTTTCGTGAACGA+GGG 0.656799 4.2:+24524624 MS.gene39631:CDS
CTGAGAAGCTCATCTCACGT+TGG 0.660450 4.2:+24531720 MS.gene39631:CDS
GAAGGTTGTGCCGTACGAGG+CGG 0.663173 4.2:+24528787 MS.gene39631:CDS
CAGGTTCAATGAGGAAAGTG+CGG 0.674947 4.2:+24530591 MS.gene39631:intron
TGGAAGGGTGTAGAGAAAAG+CGG 0.685152 4.2:-24531344 None:intergenic
CACATGGCCTAGTACAAAGG+GGG 0.688219 4.2:+24524376 MS.gene39631:CDS
CTTTGACGTAGATGGAATCG+GGG 0.694451 4.2:-24524151 None:intergenic
GCCGGTACACCTTACCACGA+CGG 0.697770 4.2:+24524221 MS.gene39631:CDS
CAAAGAAGAACAGACCGTCG+TGG 0.697817 4.2:-24524235 None:intergenic
CTGCGCCGACGATAACGGCG+CGG 0.710183 4.2:-24524199 None:intergenic
ACACATGGCCTAGTACAAAG+GGG 0.725388 4.2:+24524375 MS.gene39631:CDS
TTCCACTGATGTTGTCACTG+TGG 0.733192 4.2:-24524055 None:intergenic
CGAGGCGGTAACATCTACTG+GGG 0.743069 4.2:+24528802 MS.gene39631:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.2 gene 24524026 24532312 24524026 ID=MS.gene39631
chr4.2 mRNA 24524026 24532312 24524026 ID=MS.gene39631.t1;Parent=MS.gene39631
chr4.2 exon 24524026 24524688 24524026 ID=MS.gene39631.t1.exon1;Parent=MS.gene39631.t1
chr4.2 CDS 24524026 24524688 24524026 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24524836 24524986 24524836 ID=MS.gene39631.t1.exon2;Parent=MS.gene39631.t1
chr4.2 CDS 24524836 24524986 24524836 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24528728 24528968 24528728 ID=MS.gene39631.t1.exon3;Parent=MS.gene39631.t1
chr4.2 CDS 24528728 24528968 24528728 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24530594 24530651 24530594 ID=MS.gene39631.t1.exon4;Parent=MS.gene39631.t1
chr4.2 CDS 24530594 24530651 24530594 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24530937 24531017 24530937 ID=MS.gene39631.t1.exon5;Parent=MS.gene39631.t1
chr4.2 CDS 24530937 24531017 24530937 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24531128 24531163 24531128 ID=MS.gene39631.t1.exon6;Parent=MS.gene39631.t1
chr4.2 CDS 24531128 24531163 24531128 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24531261 24531500 24531261 ID=MS.gene39631.t1.exon7;Parent=MS.gene39631.t1
chr4.2 CDS 24531261 24531500 24531261 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24531600 24531741 24531600 ID=MS.gene39631.t1.exon8;Parent=MS.gene39631.t1
chr4.2 CDS 24531600 24531741 24531600 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
chr4.2 exon 24531831 24532312 24531831 ID=MS.gene39631.t1.exon9;Parent=MS.gene39631.t1
chr4.2 CDS 24531831 24532312 24531831 ID=cds.MS.gene39631.t1;Parent=MS.gene39631.t1
Gene Sequence

>MS.gene39631

ATGGTTTCAGACTATTCAGATCACAAATTCCACAGTGACAACATCAGTGGAAACTGCTTCAGAGATACAAAAAGCGCCCTCTACAAACGAATCATGAAAGAGTGGAAAATTCTCGAAAACAATCTCCCCGATTCCATCTACGTCAAAGCCTATGAAACCCACATCGATTTACTCCGCGCCGTTATCGTCGGCGCAGCCGGTACACCTTACCACGACGGTCTGTTCTTCTTTGACATTCAATTTCCTGCCGATTACCCAAACAATCCACCAAAGATTCATTATCACTCTTTCGGGTACAGCTTAAACCCTAATCTTTACCCTGACGGCACTGTTTGCTTAAGCCTTCTCAACACATGGCCTAGTACAAAGGGGGAGAAGTGGGACCCATCTAGGTCTACTCTTCTTCAGCTTTTAGTTTCTATGCAAGCACTTGTTCTTAACGAGTACCCACTTTTTAACGAACCTCTTTACCGTATATTCAATCGTTCCTTTCATGAAGCCAAGTCACTTACTTACAATGAAGATGTTTTTGTTAAAACATGTCACACTGCATATTTGATCATTCGGACACCGCCAAAGAACTTTGAGGAATTTGTTAAGGAACACTTTCGTGAACGAGGGCATGTTCTTCTTGCTGCGTGTAAAGAGTACGTGAATGGGCGTGTGAGGGTTGGGTATTATGGTTACAACATCAAGGACACCAATAGCAGCAGCAGCAACAACAACAACAACAATTCTTCGATAGTTATTGAGGTTAGGGAGTCTTTTCAGGTGTCGTTACGGCATGCTTACCGCCGTTTGTATACAAAGTTTAAGGAGTGTGGTGCTTCGTTGGAGACTTTCCATCATGACTTGGAGTTTGAAGTACAAGTCAAGAGCATGAAATCCAATGCTGGGAATGGAATCTTCAACAATGCAATGGCAATGATCAAACAAGCTTTGGGATGGACGAAGGAGCAGAGTACCTAATAACTAACTAACTGCATGCATGATCATGGTTATTTTTTAATTATTCAAGTCTCTTTTTGTTTTATTTTCTTATGTTGGAGTTTGTTTTTCAATGAATGCTAGGGACACTCACTTTTCAACATTTATGTAACACTCACTCTCTTATTGGATGGAATTAATGTATATCCACCTTAAATGAAACTCATTTCCAAATTGTGAAACCCAACTTAATTTAAACCAGCTAGGAAATGAGTGTTTAGCAGAGTGTTGTTAGCACCACTCTTGTTTTTCGGAAGTCTCTCTAGAGTATGATATGTTTCTAGTTTCAATTTATAATGAGATGTTTCTAGTTTCAATTTATGAATGAAGTTAATTTGTTCCTTAATTTTTTTCTCTGTTTTGTGTTGTGTTTGATTTTTACTTCTTTTTATAAATTGAAGTGTATTTGTGCTTGTGATTTGATGTGACTTGGTAGAGGTGAGGTTAGTATAGTTTGAAATTTTATATGGTTTCATTTCATCTTCATGCGATAAGTAAGAAACAATTGGCTGTATTTCCTTCAAAAAAAATTGGTTGTATATTTAATAGTAAATACGGTCTTTGAAGAATATAACATATATATAATCTTGGTTTGAAATTATACTATATAGTGATGAAAGGGAATTAAATATCTCATCAACAAATGAAATCGATCACCAATTTAAGATTCAAATTAGATCTTTGTTGGATTCTAAGTTTACAGGTTTATTCACCTTTTAAAAAAATTTATAGGTTTATTCTCTTAAGTCTATGCTGACAGTGCAAGAAATACTCAAAATCGAATAAAGTTGTGTAAATACAATAAAGAACAAGTACTAAGTATAACAAATGAAGGAGAAAAGTAATAAAAAAAAGTTTAAGAGAATGGTATTATTAGTATTATTACTACCAAGAGTGAGTTACATACAGTTAAAACTCTATAAATTAATAATGTCAGGACCGGAGAAATTTATTAATTTAGAGAGTTATTAATTTATCGATAAATTAATAATTATTAATTTAAAGAGTTTTTAAGTAATTATACTGTACATACCAAACAAAAAGGCAAATTACGTTGCAGCGAACCTTGGGACTCAATGTAAATTAGTAACTACTGTGTGCATATGCATTGGAAATCAATAATGACTTTTGAAGTACAGTAAATGTAAACAAGAACAAATGTGTTCAATGTATTCTTAATATATTTTTTTCAGTTTCTATGAATTATTAATTTATGATTTTCTTGGGACCGAAAATTATAAAGGGATCTCCCGAAAAATTATTATCTTATTATTTTATCGAGTTTTTCAATTTTTTACATTGGCCCAAGTCGGGACCGGACAAATTTATTATTTTAGAGAGTTTATTAATTTACCGAGTATTAATTCAAAGAGTTTCTACTGTACATCAATCAAACAATCAATAACATGTTTATCCTTTAAGGGATGTGTGATATAATATGCAATCTTGCATATGAAAATGAACGTCCGAATACAGAGAACAAATCTCATATTTATAGTAAAAGAAAGAAACTAACTTAAGTTTTCATGCATGACATGTGTCTTCCTAACTACTTAAAAACTTCTTTTGCAAGTTTTTTCTTGTATTCGAATTTTGATCTTTGATGGCCTCTGTTTATACCTAAAAATGATTATCTTGCTAGCAAAAATCGACAAATATGTGATTAAGGCGGATAAGTGTGTTTCCTCTCAGTTGATGGAAGGTTAGACAAAGGTTATAACAACCAAGCTAAAGATATATGTTAAAGCTCAATCTAAGTTAAACCTCATTAGAATTGGATAATGAGGCATCCAACCCACACTCAATGTTTACGGTGCCACTAGCTGCACGGAGAAAAGAGTAAGGAACCATGTCGTGAAAGTTGCGCATTGTGGATCTTGTCAAGGGATCATACCGTAAAGTGGGAAGCTGACAATCGAATGTGGGGTGTCATGAACCCTTCAATGAGGAAAAGTAACACATTACGAGCGAAAATAGTCGAGTAAGACTAGACATCGTGGGTCATGTTGTTTCGGCTAATAGAAATAGTTTTGCATATAAATACCTACTTGGTACAACATATAACATATGATCAATCAAACACATGATATTTTCACCATTTTTTAATCCTTTATTATTCAATATTTATCTTTATTTTACATGCATTTCTTAGTTTAGTAAAAACTAAAAAACAACCATACTTAATGCTCTACTAGAGAAAATAAGGAACCCACATCTTCCTTCCTCAATGTCAATTGTTTGAAGAAATGGAATCAAATTTGATAAATCAAACGACTCTTTGACTAATTTCTAGCTCCATCAATAAATTTGGCACGCCCTGTGGGACAATACAACTTTTACCATTGAAAAATTCAGTTCTTTAGTTGAAGACCATGTTTGGTGTATGTATTTATAATCAGGAAGTTTTTTTGGATGCATGGATCAACCGTTATCGTCAACATAAAGAAGGCTAGACCAAAACTGAAATGAGCCTTAACAAGCTTTAGGAATGTGAACATATGAGGCTCCTCCTAGTGTTTTTGAGGCACCACCAATTGGTCCACCTGAGACTCTGGTAGTAATGTTAATATCTTTCACTGGCCAAGATATTGACATAAGCATATGTGACATCATCAATCAAGTCTCTCAAGAACTTGCTAACATTGTTAAACTCATTGTTCATAACGAGATTCAAAAAGACAATAAGTTGGAGGCCAGACAAACTTATTACGATGATAATTCTATCACCTATGCCCCATGAGGAGTTTAAGGGAATGAAGGTACCTTCTACTTCAGTCAAACAAATTCAGTCGATCATTGCTTTGATAGGACCTTAATAGGTTCATTCGATACATATACAGAAGGTACCTTCTACTTCTTATAATTCCCAGTCTAGTATTTTTGTCATAACTAACCTTAGGAGGCACTCATTGATGAATGATACAATCATGCTAATCTTCAAGTTGGACATGTATATATTACAAAGAGATCACCTACCGTAGTAGGACAAATTGACATTACAAAAGTGACGCCTTATTGCAATATTATGCATGCGAATAACATGTCATACGTCGGAGGCCGAATACATCTTCAGCCTAGCAAGACGATCAAAACGTCTAGTTGTAGATTACTTAATCCACCAACCATGGGCAAATCCACTTGATTTCAAAAGAATATGTTCATATTTGATTAATAAACCGAAAAAGTTAATGATCACTTTGTTTAAATTAACCATTTGGACGGTAAAAAATTGTAAATTGTAATTTTTGTTTTATATTCAAAATACCAAATTTAAATATAGATCGTCCGATCTTGATCGAACGACTCTTGAATATGTGTTGCTCTCATGAGCGGGGTCCATTATTGTGTGACTTGAAAGAGAAGGTGCATGATCATGAATCTGAAATGAGATGTGGGGTCGGTCATCTACAATAATGTTGGCTTAAGCTTCCATCACCTTGAATTGAACTCAATCTAAAATCCAAAAAAACACACGAATCCCTAAAACTAAAGGCCAATTTGGATATCCAGTGTCTACTCAGCATCACCCATCACCCACCATTTTATTATTCATTCTTATTCTTTATTACTCATAGTTAAATTAAATTAAATCTAAATATTATTGTAATCTCAACAACAACAACACAATCTTTTTCCGATTCAAAACCATCACTGTTATTTTCTGATTGACCATAGAAAGAAAAAAAAAAAAACTTCAAACTTTTGAATTTCTCAGCTGAAATTCACGTGTTTCGCTTACTGGGTCAATCTCATATCATCGCTATGCGTGGAGTTGAAGGTTGTGCCGTACGAGGCGGTAACATCTACTGGGGAAGAAAACACGACACTGATTTCAAAGGAATCGTTGTAATCTTTGCTTGGGTTTCAGTTCCACAAAACCTGTTACAGGAATTCGTTGACCTTTACTCCTCTTTAGGTTGGAATTCTCTTGTTTGTTATGCTCATTATCTATCCGCGTAAGTTTCTCTTTTCTAGCTTCTATATCATTTTTTTTTTTCAATTTCGTTGGCCATGGAATAGAATTATTGGATTTAGAGGTTGATAGATTGATGGGTTTGGTGTTACTTGTGGATGGAATTTTGTAAAAATCAGAGCTTTCTTGCAAAGTTTAGACTAAAATAATAATCTTTGTTTAGTAATTTTTGGTCATTGAAAGTATAATCATGTATGATTTTGGTTACTGATATGTTGTGTACTTAGTTCTTTGGATTATCGATCAGAATTATTATATTTTGGAGGTTATTTTGATCCTTCTAGTTAAGTGTTTTTGGGTTTTACTTTGCCTTAAGCTTGTGAGAGGGGAGCTTGTATGAATTGCTTGTTATGTTTGCCTAACCATTTGGAATGGTTATAGCAACAAAAACTACCTTGGATGATGCTTTGCATGTTAAATTAGCATAATGATTTTTAATGTCAAACGTTTGATCGTATGCTGGTACCTTTTCTTAGCATTAGTTGTCTGGCTGAGAATTACAAAGAAGAGAAGTGAAATTTGCGAGGTTAATAGATAGGTGGTAGAAAGTAGAAACTATATGCTGGTCTGCTCTGGCAACTTGAACAGGGGACCATTTGTTGAGAATAGAATAAGTTACTCCAAAGATTGGGTGGGTTAGTAATGTATGTGTAGCTAGCTTATACAGACTTGTGAAATTAAGAATATGGGTAGGTTGGAGAGGTTGAAATTGTTTCTTTACGTATTTAATTCATACCAAAATATAGCATTGAATCTTGTAAGACAGCCATGGATTCAGCTGGAACACTTGTGGAGGGTTTGTGATTTCATTTTTATTCCTATAGGAATGTAAGAGACTAGTCTGAATATGCATTTGTTTAGTTGCTTCAATTTCTGGTTGAAGTGCTCTATGACAAAGCTCAAATAGTTCGATTTCAAGAAGAAAAAAATGTTCTACTTCACTAGTTAAGTTAATGATATTTAGAATGACAAGGTTATTTTTAGTTTTTTACTATTATCCCGCAACAGCATATTAGTATATGATCGAAACAGTAAAAATCAAGCTACGAAACTGTGTATTATTGAATAGTAAAGATGAGCTCAAGAGCTCTACCGTGGTGGAAAATAAAAAAGGAAGGGATAAGCCAGAGTTTCGAGAACTTCTAACCAGAGCTAAATGCTCCTAAACCAGAGAAAATATTCCCCTAACCAACTCTAAAAATATTCAGAATTCCCCTTTCCACTACCCAACCCCCTCACATGAGATTGGTGCACACCTCACTAACTCACTCATCCTCTCCCCCTTGCCCCACATTTTTTCCCTTCGTGCAAACCTTCCAGAAGCTGGCTCGTGCTGGTTTGCCCCATTGCTTAGCTCACCATGGGATATAGGTCCAAGTGTCCTATCAGTATACTTGTAAGTTATATGTAGCTTGGTGTTTTGCTATCCTAAATCTGTTCTTACAAATACTTATGTGCGCTCAATTCATAACCAACTAAGTATCTATTGGTTACACGTTATTTTTTTGAATAAGGTCACCACCGTATTAAATTTGTACTCACCTTGACTCATTGGTTTGGTGTACAATACTGATGGAATTTTTATATCACAATTTTCTCTTCATCCAGGTTCAATGAGGAAAGTGCGGTGCCATTGGCATTTTATGTTTTTGATGAACTTATTGAGGTTAGTTGCGAAATTTTCAAAACATGCTAAAATTTATAGCAATGCTCTTGTCAATTTACAATTAATTAGGAAAATGCTAACAAGCACCCTTGGGGCACTTGTTTAGAAGTCAATAGAAGAAACTTTTTCTTGGAAATTGTGTATTCAACACATTGAAACTTAAAAAAAGAACTTTTTCTACATAAAAATTATAAATCATTTCCTCTTTTGATTTCTTAACAAGTGCCCTAAGTGTCATAGTCATAGTCATAGGTTTAAGTGAAAAGATTGCTTTGGGTTACGCAGGTGCTAAGGACTAGGCCATGTCCTGTTGTGTTTGCTTCTTTTTCTGCTGGGTCGAAAGCCTGTCTGTACAAATTATTTCAGGTAATTTCAGTCATCCAATTTTTATCTTTGGTATGAAGAATATTATTTGAAGTTGTGTATGTCTTTTGGGGTAGAAATGCCTACTATAATTGCTTTCCATACAAATGCAGCTTAGTGAAGGAAGATGCACAGCTCCACTCAACTTGGTAATGCAATTTTCAAACCTCGCTTTCTACTTTGATTGATGTGGTTTTCTACATCGCGGCCATTGTGGAATTTATTAATTCATTTGTTATTTCGTAGCATGACTGTCAATTGTTTAGGAACTGTGTTTCTGGACATATATATGATTCTGGTCCACTAGATGTTACAAGTGATTTTGGCTTCCGCTTTTCTCTACACCCTTCCATTGCAAAAGTGCCCGGACCATCAAAGCTTGTTTCTTGGGTAGCAAAGTCTGTTGCATCTGGTTTGGATGCTTTATACTTGACTAGATTTGAATCTCAATCAGCTGATCATTGGCAGGCTTTATATTCTTCTGTTGTAAGTAATCTATCTTGACCATCTGGAATGTTTAGTGCCATGTTTCGGTTTTTATTTTTTACTTTCGTAAATTAAACCTAATTTCATTGTTGTCTTCAGAATTTTGGGGCTCCATTTCTCATTTTATGTTCGGAGAATGATGAACTTGTGAGATATCAAAGTATCTATGATTTTGCTCAACGATTACGCAATCTCAACGCTGATGTCAATCTTGTAAATCTGAGAAGCTCATCTCACGTTGGTTAGTCTGAGACCCATGTTAAATTATGTTCTGGCAAGCTTGAACGAGTTTAACAGAGTTATCATAATAGTTATTATTATCTGATTCAGGTCATTATGAACATCATCCAATCCAATACAGAGCTGCTGTGAGCCATTTATTGGAGAAGGCCGTGTCAACGTATTCACGAAAAGTTATACTTGAACGAGAAAGAACTGGTATTGATGGTACGCACGACGAGATATCTGAGTTAATCTGTGACCTGCAGAAAGTTGCAATTAATTCAAATGAGAGTTTTAGAAGAGTTGCAGTGGGACCAAGTGACCACTTCTTTTTGCCTAGTTCAGCAGGACATAACAACAATGATAGAGAATCTGGGATTCCACGGGACGAACAGAAAGAAGAACCCGTTTGTGCTCCTAGCTTCCCAAGCATCAGCGCTCACAGCGTCCTTGGGCAATTTCTTTTTGACGTTTGTGTTCCAAAGAATGTTGAAGGTTGGGATGATGTGAAATTTTGTGGGAATCGGAATGGGAGGTCACGTGTGTCACCTTTTAGAGGAATTAAACGCATTGGTCGCTCTAGGTTATGA
Protein sequence

>MS.gene39631.t1

MVSDYSDHKFHSDNISGNCFRDTKSALYKRIMKEWKILENNLPDSIYVKAYETHIDLLRAVIVGAAGTPYHDGLFFFDIQFPADYPNNPPKIHYHSFGYSLNPNLYPDGTVCLSLLNTWPSTKGEKWDPSRSTLLQLLVSMQALVLNEYPLFNEPLYRIFNRSFHEAKSLTYNEDVFVKTCHTAYLIIRTPPKNFEEFVKEHFRERGHVLLAACKEYVNGRFKECGASLETFHHDLEFEVQVKSMKSNAGNGIFNNAMAMIKQALGWTKEQTEIHVFRLLGQSHIIAMRGVEGCAVRGGNIYWGRKHDTDFKGIVVIFAWVSVPQNLLQEFVDLYSSLGWNSLVCYAHYLSAFNEESAVPLAFYVFDELIEVLRTRPCPVVFASFSAGSKACLYKLFQLSEGRCTAPLNLHDCQLFRNCVSGHIYDSGPLDVTSDFGFRFSLHPSIAKVPGPSKLVSWVAKSVASGLDALYLTRFESQSADHWQALYSSVNFGAPFLILCSENDELVRYQSIYDFAQRLRNLNADVNLVNLRSSSHVGHYEHHPIQYRAAVSHLLEKAVSTYSRKVILERERTGIDGTHDEISELICDLQKVAINSNESFRRVAVGPSDHFFLPSSAGHNNNDRESGIPRDEQKEEPVCAPSFPSISAHSVLGQFLFDVCVPKNVEGWDDVKFCGNRNGRSRVSPFRGIKRIGRSRL