Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40051.t1 | XP_013468641.1 | 99.2 | 126 | 1 | 0 | 1 | 126 | 1 | 126 | 1.70E-65 | 258.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40051.t1 | Q8L8Q8 | 82.4 | 125 | 22 | 0 | 1 | 125 | 1 | 125 | 2.2e-58 | 226.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40051.t1 | A0A072VML2 | 99.2 | 126 | 1 | 0 | 1 | 126 | 1 | 126 | 1.2e-65 | 258.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056314 | MS.gene40051 | 0.801711 | 7.91E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40051.t1 | MTR_1g073620 | 99.206 | 126 | 1 | 0 | 1 | 126 | 1 | 126 | 5.01e-90 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40051.t1 | AT5G64816 | 82.500 | 120 | 21 | 0 | 1 | 120 | 1 | 120 | 2.39e-74 | 217 |
| MS.gene40051.t1 | AT5G64816 | 82.500 | 120 | 21 | 0 | 1 | 120 | 1 | 120 | 2.39e-74 | 217 |
| MS.gene40051.t1 | AT5G64816 | 82.500 | 120 | 21 | 0 | 1 | 120 | 1 | 120 | 2.39e-74 | 217 |
| MS.gene40051.t1 | AT5G64816 | 82.500 | 120 | 21 | 0 | 1 | 120 | 1 | 120 | 2.39e-74 | 217 |
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATATTTGGTGGTCACTTT+TGG | 0.138181 | 1.4:-60128738 | MS.gene40051:CDS |
| TTCAAAGAGGATGTTGAAAA+AGG | 0.272946 | 1.4:-60128579 | MS.gene40051:CDS |
| ACCAACATCAAGTACCTAAT+TGG | 0.274343 | 1.4:-60128451 | MS.gene40051:CDS |
| GATACTTGTGTTACTGTTTG+TGG | 0.347747 | 1.4:-60128527 | MS.gene40051:CDS |
| GCAGTCCCTGTCGTTGTTGC+AGG | 0.348680 | 1.4:-60128710 | MS.gene40051:CDS |
| TATCAGAGATGGGAGATATT+TGG | 0.353189 | 1.4:-60128751 | None:intergenic |
| ATTGGAATGACACATGTCTT+AGG | 0.381625 | 1.4:-60128433 | MS.gene40051:CDS |
| AGACATGTGTCATTCCAATT+AGG | 0.386451 | 1.4:+60128437 | None:intergenic |
| GTAACACAAGTATCAGGAAT+TGG | 0.406394 | 1.4:+60128536 | None:intergenic |
| CAAACAGTAACACAAGTATC+AGG | 0.430522 | 1.4:+60128530 | None:intergenic |
| GCTTGTCCTGCAACAACGAC+AGG | 0.451940 | 1.4:+60128704 | None:intergenic |
| TCCAATTAGGTACTTGATGT+TGG | 0.474351 | 1.4:+60128450 | None:intergenic |
| AAGCATTTAGAGTTAAGAAA+AGG | 0.483524 | 1.4:-60128685 | MS.gene40051:CDS |
| TGCTGAAGCTTGTGCTCGTA+CGG | 0.484272 | 1.4:-60128483 | MS.gene40051:CDS |
| AGCAAAGGTTGAAGAGTGCA+AGG | 0.518677 | 1.4:-60128652 | MS.gene40051:CDS |
| AAAGGAATGCAGAGGAGCAA+AGG | 0.541153 | 1.4:-60128667 | MS.gene40051:CDS |
| AGAGTGCAAGGGGAAGAGAA+AGG | 0.552353 | 1.4:-60128640 | MS.gene40051:CDS |
| CAGAGATGGGAGATATTTGG+TGG | 0.589138 | 1.4:-60128748 | None:intergenic |
| CAAAGGTTGAAGAGTGCAAG+GGG | 0.608201 | 1.4:-60128650 | MS.gene40051:CDS |
| GCAAAGGTTGAAGAGTGCAA+GGG | 0.610878 | 1.4:-60128651 | MS.gene40051:CDS |
| AGTTAAGAAAAGGAATGCAG+AGG | 0.619043 | 1.4:-60128675 | MS.gene40051:CDS |
| CTTGTCCTGCAACAACGACA+GGG | 0.623317 | 1.4:+60128705 | None:intergenic |
| GGAATGACACATGTCTTAGG+AGG | 0.635618 | 1.4:-60128430 | MS.gene40051:CDS |
| AAAGGGTCTGCACTTCAAAG+AGG | 0.655293 | 1.4:-60128592 | MS.gene40051:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGCATTTAGAGTTAAGAAA+AGG | - | chr1.4:60128443-60128462 | MS.gene40051:CDS | 25.0% |
| !! | ATGAAATTTTCGTTTGTGAA+AGG | - | chr1.4:60128518-60128537 | MS.gene40051:CDS | 25.0% |
| !! | TGAAATTTTCGTTTGTGAAA+GGG | - | chr1.4:60128519-60128538 | MS.gene40051:CDS | 25.0% |
| TTCAAAGAGGATGTTGAAAA+AGG | - | chr1.4:60128549-60128568 | MS.gene40051:CDS | 30.0% | |
| AAGAGGATGTTGAAAAAGGT+TGG | - | chr1.4:60128553-60128572 | MS.gene40051:CDS | 35.0% | |
| ACCAACATCAAGTACCTAAT+TGG | - | chr1.4:60128677-60128696 | MS.gene40051:CDS | 35.0% | |
| AGTTAAGAAAAGGAATGCAG+AGG | - | chr1.4:60128453-60128472 | MS.gene40051:CDS | 35.0% | |
| ATTGGAATGACACATGTCTT+AGG | - | chr1.4:60128695-60128714 | MS.gene40051:CDS | 35.0% | |
| CAAACAGTAACACAAGTATC+AGG | + | chr1.4:60128601-60128620 | None:intergenic | 35.0% | |
| GTAACACAAGTATCAGGAAT+TGG | + | chr1.4:60128595-60128614 | None:intergenic | 35.0% | |
| TCCAATTAGGTACTTGATGT+TGG | + | chr1.4:60128681-60128700 | None:intergenic | 35.0% | |
| ! | AGACATGTGTCATTCCAATT+AGG | + | chr1.4:60128694-60128713 | None:intergenic | 35.0% |
| ! | GATACTTGTGTTACTGTTTG+TGG | - | chr1.4:60128601-60128620 | MS.gene40051:CDS | 35.0% |
| !! | AGATATTTGGTGGTCACTTT+TGG | - | chr1.4:60128390-60128409 | MS.gene40051:CDS | 35.0% |
| !!! | ATATTTGGTGGTCACTTTTG+GGG | - | chr1.4:60128392-60128411 | MS.gene40051:CDS | 35.0% |
| !!! | GATATTTGGTGGTCACTTTT+GGG | - | chr1.4:60128391-60128410 | MS.gene40051:CDS | 35.0% |
| !!! | TATTTGGTGGTCACTTTTGG+GGG | - | chr1.4:60128393-60128412 | MS.gene40051:CDS | 40.0% |
| AAAGGAATGCAGAGGAGCAA+AGG | - | chr1.4:60128461-60128480 | MS.gene40051:CDS | 45.0% | |
| AAAGGGTCTGCACTTCAAAG+AGG | - | chr1.4:60128536-60128555 | MS.gene40051:CDS | 45.0% | |
| GGAATGACACATGTCTTAGG+AGG | - | chr1.4:60128698-60128717 | MS.gene40051:CDS | 45.0% | |
| ! | AGCAAAGGTTGAAGAGTGCA+AGG | - | chr1.4:60128476-60128495 | MS.gene40051:CDS | 45.0% |
| ! | CAAAGGTTGAAGAGTGCAAG+GGG | - | chr1.4:60128478-60128497 | MS.gene40051:CDS | 45.0% |
| ! | GCAAAGGTTGAAGAGTGCAA+GGG | - | chr1.4:60128477-60128496 | MS.gene40051:CDS | 45.0% |
| AGAGTGCAAGGGGAAGAGAA+AGG | - | chr1.4:60128488-60128507 | MS.gene40051:CDS | 50.0% | |
| CTTGTCCTGCAACAACGACA+GGG | + | chr1.4:60128426-60128445 | None:intergenic | 50.0% | |
| TGCTGAAGCTTGTGCTCGTA+CGG | - | chr1.4:60128645-60128664 | MS.gene40051:CDS | 50.0% | |
| GCTTGTCCTGCAACAACGAC+AGG | + | chr1.4:60128427-60128446 | None:intergenic | 55.0% | |
| GCAGTCCCTGTCGTTGTTGC+AGG | - | chr1.4:60128418-60128437 | MS.gene40051:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 60128385 | 60128765 | 60128385 | ID=MS.gene40051 |
| chr1.4 | mRNA | 60128385 | 60128765 | 60128385 | ID=MS.gene40051.t1;Parent=MS.gene40051 |
| chr1.4 | exon | 60128385 | 60128765 | 60128385 | ID=MS.gene40051.t1.exon1;Parent=MS.gene40051.t1 |
| chr1.4 | CDS | 60128385 | 60128765 | 60128385 | ID=cds.MS.gene40051.t1;Parent=MS.gene40051.t1 |
| Gene Sequence |
| Protein sequence |
