Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40372.t1 | XP_003603557.1 | 100 | 147 | 0 | 0 | 8 | 154 | 178 | 324 | 8.80E-69 | 269.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40372.t1 | Q94B65 | 81.6 | 147 | 27 | 0 | 8 | 154 | 177 | 323 | 6.6e-57 | 221.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40372.t1 | G7J2T9 | 100.0 | 147 | 0 | 0 | 8 | 154 | 178 | 324 | 6.3e-69 | 269.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene40372 | 0.807245 | 5.55E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATACAGATAAAGAATA+TGG | - | 24208:708-727 | MS.gene40372:intergenic | 15.0% |
| !! | ATAATAAGAAACATCAAAAA+CGG | - | 24208:669-688 | MS.gene40372:intergenic | 15.0% |
| !! | ATAATACAGATAAAGAATAT+GGG | - | 24208:707-726 | MS.gene40372:intergenic | 15.0% |
| !! | ATTATACAATATAGACAAAT+AGG | - | 24208:1360-1379 | MS.gene40372:intergenic | 15.0% |
| !!! | TTATTTTGAGTGAAAAAAAT+AGG | - | 24208:1120-1139 | MS.gene40372:intergenic | 15.0% |
| !! | AAATACAAACAAAAGATGAA+TGG | - | 24208:1899-1918 | MS.gene40372:intergenic | 20.0% |
| !! | AATCTTGTTGAAAGAAAAAT+AGG | + | 24208:1220-1239 | MS.gene40372:intron | 20.0% |
| !! | ATCTTGTTGAAAGAAAAATA+GGG | + | 24208:1221-1240 | MS.gene40372:intron | 20.0% |
| !! | TTTATCTGTATTATTTGCAA+AGG | + | 24208:713-732 | MS.gene40372:CDS | 20.0% |
| !!! | ATTTTGATCTACTTACTATT+AGG | + | 24208:945-964 | MS.gene40372:intron | 20.0% |
| !!! | TCAGTTCTAAATTATATCAT+TGG | + | 24208:1436-1455 | MS.gene40372:intron | 20.0% |
| ! | AAGCACAAAACATTTAGTAT+AGG | - | 24208:977-996 | MS.gene40372:intergenic | 25.0% |
| ! | AGAACCTAATATTGATACAT+AGG | + | 24208:1867-1886 | MS.gene40372:intron | 25.0% |
| ! | AGCACAAAACATTTAGTATA+GGG | - | 24208:976-995 | MS.gene40372:intergenic | 25.0% |
| ! | TATGCCTATGTATCAATATT+AGG | - | 24208:1874-1893 | MS.gene40372:intergenic | 25.0% |
| ! | TTGAAAGAAAAATAGGGAAA+GGG | + | 24208:1227-1246 | MS.gene40372:intron | 25.0% |
| !! | TTAATTTTGCCATGATAAGA+CGG | + | 24208:1313-1332 | MS.gene40372:intron | 25.0% |
| !! | TTGTATAAACTTTGTTGTGA+TGG | + | 24208:1494-1513 | MS.gene40372:intron | 25.0% |
| !!! | GAGTTATTCTTTTTCATTTC+TGG | + | 24208:1676-1695 | MS.gene40372:intron | 25.0% |
| !!! | TTTTGAGTGAAAAAAATAGG+TGG | - | 24208:1117-1136 | MS.gene40372:intergenic | 25.0% |
| AAAGAAACTGCTAAAAGTCT+TGG | + | 24208:912-931 | MS.gene40372:intron | 30.0% | |
| AAATGCCAACATTCAAAACA+TGG | - | 24208:785-804 | MS.gene40372:intergenic | 30.0% | |
| ACAAATAGGTGTTCAATTAC+TGG | - | 24208:1346-1365 | MS.gene40372:intergenic | 30.0% | |
| ATGTTTCTTATTATAGCCAC+AGG | + | 24208:675-694 | MS.gene40372:CDS | 30.0% | |
| GAAAAAAAGTAAGACAGTGA+AGG | + | 24208:2093-2112 | MS.gene40372:CDS | 30.0% | |
| GCACAAAACATTTAGTATAG+GGG | - | 24208:975-994 | MS.gene40372:intergenic | 30.0% | |
| GTTGAAAGAAAAATAGGGAA+AGG | + | 24208:1226-1245 | MS.gene40372:intron | 30.0% | |
| TCTAGTGACAGAAAATTCTA+TGG | - | 24208:1850-1869 | MS.gene40372:intergenic | 30.0% | |
| TGTTTCTTATTATAGCCACA+GGG | + | 24208:676-695 | MS.gene40372:CDS | 30.0% | |
| ! | AATTTAAGTTTGTGGGAACT+CGG | + | 24208:1179-1198 | MS.gene40372:intron | 30.0% |
| ! | TAATTTTGCCATGATAAGAC+GGG | + | 24208:1314-1333 | MS.gene40372:intron | 30.0% |
| ! | TGGAGAGAATTTAAGTTTGT+GGG | + | 24208:1172-1191 | MS.gene40372:intron | 30.0% |
| ! | TTATTGTTTCTTCAGACCTT+TGG | + | 24208:1968-1987 | MS.gene40372:intron | 30.0% |
| ! | TTGGAGAGAATTTAAGTTTG+TGG | + | 24208:1171-1190 | MS.gene40372:intron | 30.0% |
| AAAATAACACCTACCTGTTG+AGG | + | 24208:1133-1152 | MS.gene40372:intron | 35.0% | |
| AACAATAGTGCACAAGAACA+TGG | - | 24208:1747-1766 | MS.gene40372:intergenic | 35.0% | |
| GAAAAAGAATAACTCTGCGA+AGG | - | 24208:1671-1690 | MS.gene40372:intergenic | 35.0% | |
| GAAAATTCTATGGCAAACAC+AGG | - | 24208:1840-1859 | MS.gene40372:intergenic | 35.0% | |
| GCTTTAACTACAACGATTGT+GGG | + | 24208:1773-1792 | MS.gene40372:CDS | 35.0% | |
| GTTTCTTATTATAGCCACAG+GGG | + | 24208:677-696 | MS.gene40372:CDS | 35.0% | |
| TGCTTTAACTACAACGATTG+TGG | + | 24208:1772-1791 | MS.gene40372:CDS | 35.0% | |
| ! | ACCTTTGGTTTCTTCTTACT+GGG | + | 24208:1983-2002 | MS.gene40372:CDS | 35.0% |
| ! | ATTTAAGTTTGTGGGAACTC+GGG | + | 24208:1180-1199 | MS.gene40372:intron | 35.0% |
| ! | TAGTATCTGATAACGTGACA+TGG | + | 24208:1576-1595 | MS.gene40372:intron | 35.0% |
| !! | CTTATTCTTTCGTTGGTGAT+GGG | + | 24208:1704-1723 | MS.gene40372:CDS | 35.0% |
| ACCCAGTAAGAAGAAACCAA+AGG | - | 24208:1987-2006 | MS.gene40372:intergenic | 40.0% | |
| ACGATTGTGGGTGTTCTTAA+AGG | + | 24208:1785-1804 | MS.gene40372:CDS | 40.0% | |
| AGTGAAGGTAGTTACAGATG+TGG | + | 24208:2108-2127 | MS.gene40372:CDS | 40.0% | |
| ATCCAGTCACATTCAAAGCA+TGG | - | 24208:2021-2040 | MS.gene40372:intergenic | 40.0% | |
| CCATCACCAACGAAAGAATA+AGG | - | 24208:1706-1725 | MS.gene40372:intergenic | 40.0% | |
| GGTGATCCTTATTCTTTCGT+TGG | + | 24208:1697-1716 | MS.gene40372:CDS | 40.0% | |
| TGAAATTACCTGAGTGTGGT+GGG | + | 24208:1252-1271 | MS.gene40372:intron | 40.0% | |
| ! | ACCATAGCTTGCAACATATC+TGG | + | 24208:1529-1548 | MS.gene40372:intron | 40.0% |
| ! | CCATAGCTTGCAACATATCT+GGG | + | 24208:1530-1549 | MS.gene40372:intron | 40.0% |
| ! | CCTTATTCTTTCGTTGGTGA+TGG | + | 24208:1703-1722 | MS.gene40372:CDS | 40.0% |
| ! | GACCTTTGGTTTCTTCTTAC+TGG | + | 24208:1982-2001 | MS.gene40372:intron | 40.0% |
| ! | TCTTAAAGGAGTTGGTTCCA+CGG | + | 24208:1799-1818 | MS.gene40372:CDS | 40.0% |
| ! | TGAGAATGGACTTTCATCAG+TGG | + | 24208:611-630 | MS.gene40372:CDS | 40.0% |
| ! | TGCTTTGAATGTGACTGGAT+TGG | + | 24208:2021-2040 | MS.gene40372:CDS | 40.0% |
| ! | TTTGGTTTCTTCTTACTGGG+TGG | + | 24208:1986-2005 | MS.gene40372:CDS | 40.0% |
| AAACCTCTCAAACCAGACTG+AGG | - | 24208:1082-1101 | MS.gene40372:intergenic | 45.0% | |
| AGAATATGGGAATTCCCCTG+TGG | - | 24208:694-713 | MS.gene40372:intergenic | 45.0% | |
| ATAACACCTACCTGTTGAGG+AGG | + | 24208:1136-1155 | MS.gene40372:intron | 45.0% | |
| CCCAGATATGTTGCAAGCTA+TGG | - | 24208:1533-1552 | MS.gene40372:intergenic | 45.0% | |
| GAAATTACCTGAGTGTGGTG+GGG | + | 24208:1253-1272 | MS.gene40372:intron | 45.0% | |
| GAAGGTAGTTACAGATGTGG+AGG | + | 24208:2111-2130 | MS.gene40372:CDS | 45.0% | |
| GAGTTGTCAGGCAACTCAAA+GGG | + | 24208:1023-1042 | MS.gene40372:intron | 45.0% | |
| GGAAAAGACCCGTCTTATCA+TGG | - | 24208:1325-1344 | MS.gene40372:intergenic | 45.0% | |
| GGTGTGAAATTACCTGAGTG+TGG | + | 24208:1248-1267 | MS.gene40372:intron | 45.0% | |
| GTGAAATTACCTGAGTGTGG+TGG | + | 24208:1251-1270 | MS.gene40372:intron | 45.0% | |
| GTGGGTGTTCTTAAAGGAGT+TGG | + | 24208:1791-1810 | MS.gene40372:CDS | 45.0% | |
| TAACACCTACCTGTTGAGGA+GGG | + | 24208:1137-1156 | MS.gene40372:intron | 45.0% | |
| TTGGTCATCAATACAGCTGG+TGG | + | 24208:2040-2059 | MS.gene40372:CDS | 45.0% | |
| ! | GTCCATGCTTTGAATGTGAC+TGG | + | 24208:2016-2035 | MS.gene40372:CDS | 45.0% |
| !! | CGCTGCCATGTTTTGAATGT+TGG | + | 24208:777-796 | MS.gene40372:intron | 45.0% |
| !! | CTTTGAGTTGCCTGACAACT+CGG | - | 24208:1024-1043 | MS.gene40372:intergenic | 45.0% |
| !! | GGATTGGTCATCAATACAGC+TGG | + | 24208:2037-2056 | MS.gene40372:CDS | 45.0% |
| AACACCTACCTGTTGAGGAG+GGG | + | 24208:1138-1157 | MS.gene40372:intron | 50.0% | |
| AGTTTGTGGGAACTCGGGAT+TGG | + | 24208:1185-1204 | MS.gene40372:intron | 50.0% | |
| CGAGTTGTCAGGCAACTCAA+AGG | + | 24208:1022-1041 | MS.gene40372:intron | 50.0% | |
| CTCTCAAACCAGACTGAGGA+GGG | - | 24208:1078-1097 | MS.gene40372:intergenic | 50.0% | |
| GCTTGTCTTTCCGAGTTGTC+AGG | + | 24208:1011-1030 | MS.gene40372:intron | 50.0% | |
| GTTTGTGGGAACTCGGGATT+GGG | + | 24208:1186-1205 | MS.gene40372:intron | 50.0% | |
| TCTCAAACCAGACTGAGGAG+GGG | - | 24208:1077-1096 | MS.gene40372:intergenic | 50.0% | |
| TTCCACCAGCACAAGGTACA+TGG | - | 24208:581-600 | MS.gene40372:intergenic | 50.0% | |
| ! | AGTTGGTTCCACGGTAAGCT+AGG | + | 24208:1808-1827 | MS.gene40372:intron | 50.0% |
| ! | CCAGATTTTCCACCAGCACA+AGG | - | 24208:588-607 | MS.gene40372:intergenic | 50.0% |
| ! | CCTTGTGCTGGTGGAAAATC+TGG | + | 24208:585-604 | MS.gene40372:CDS | 50.0% |
| ! | GGAAAATCTGGCGCTGAGAA+TGG | + | 24208:597-616 | MS.gene40372:CDS | 50.0% |
| ! | TCAATACAGCTGGTGGTGTG+TGG | + | 24208:2047-2066 | MS.gene40372:CDS | 50.0% |
| ACACCTACCTGTTGAGGAGG+GGG | + | 24208:1139-1158 | MS.gene40372:intron | 55.0% | |
| CCTCTCAAACCAGACTGAGG+AGG | - | 24208:1079-1098 | MS.gene40372:intergenic | 55.0% | |
| GGATCACTCCTAGCTTACCG+TGG | - | 24208:1819-1838 | MS.gene40372:intergenic | 55.0% | |
| ! | ATTACCTGAGTGTGGTGGGG+TGG | + | 24208:1256-1275 | MS.gene40372:intron | 55.0% |
| ! | CCTCCTCAGTCTGGTTTGAG+AGG | + | 24208:1076-1095 | MS.gene40372:intron | 55.0% |
| ACCTACCTGTTGAGGAGGGG+GGG | + | 24208:1141-1160 | MS.gene40372:intron | 60.0% | |
| CACCTACCTGTTGAGGAGGG+GGG | + | 24208:1140-1159 | MS.gene40372:intron | 60.0% | |
| CAGACTGAGGAGGGGAAGAC+TGG | - | 24208:1069-1088 | MS.gene40372:intergenic | 60.0% | |
| CAGTCTTCCCCTCCTCAGTC+TGG | + | 24208:1067-1086 | MS.gene40372:intron | 60.0% | |
| CTTTCCACCCCACCACACTC+AGG | - | 24208:1263-1282 | MS.gene40372:intergenic | 60.0% | |
| ACCCCCCCTCCTCAACAGGT+AGG | - | 24208:1145-1164 | MS.gene40372:intergenic | 65.0% | |
| CTCAACCCCCCCTCCTCAAC+AGG | - | 24208:1149-1168 | MS.gene40372:intergenic | 65.0% | |
| CTGTTGAGGAGGGGGGGTTG+AGG | + | 24208:1147-1166 | MS.gene40372:intron | 65.0% | |
| GAGGAGGGGGGGTTGAGGTT+TGG | + | 24208:1152-1171 | MS.gene40372:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24208 | gene | 577 | 2147 | 577 | ID=MS.gene40372 |
| 24208 | mRNA | 577 | 2147 | 577 | ID=MS.gene40372.t1;Parent=MS.gene40372 |
| 24208 | exon | 577 | 734 | 577 | ID=MS.gene40372.t1.exon1;Parent=MS.gene40372.t1 |
| 24208 | CDS | 577 | 734 | 577 | ID=cds.MS.gene40372.t1;Parent=MS.gene40372.t1 |
| 24208 | exon | 1677 | 1820 | 1677 | ID=MS.gene40372.t1.exon2;Parent=MS.gene40372.t1 |
| 24208 | CDS | 1677 | 1820 | 1677 | ID=cds.MS.gene40372.t1;Parent=MS.gene40372.t1 |
| 24208 | exon | 1983 | 2147 | 1983 | ID=MS.gene40372.t1.exon3;Parent=MS.gene40372.t1 |
| 24208 | CDS | 1983 | 2147 | 1983 | ID=cds.MS.gene40372.t1;Parent=MS.gene40372.t1 |
| Gene Sequence |
| Protein sequence |
