Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40456.t1 | XP_013461923.1 | 99.3 | 134 | 1 | 0 | 1 | 134 | 543 | 676 | 8.20E-63 | 249.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40456.t1 | Q9SB75 | 77.0 | 135 | 29 | 2 | 1 | 134 | 559 | 692 | 4.6e-46 | 185.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40456.t1 | G7J2T7 | 99.3 | 134 | 1 | 0 | 1 | 134 | 554 | 687 | 5.9e-63 | 249.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053144 | MS.gene40456 | 0.801587 | 8.39E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene40456 | 0.814156 | 1.78E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40456.t1 | MTR_3g109040 | 99.254 | 134 | 1 | 0 | 1 | 134 | 554 | 687 | 8.90e-88 | 269 |
| MS.gene40456.t1 | MTR_3g109040 | 99.254 | 134 | 1 | 0 | 1 | 134 | 543 | 676 | 1.05e-87 | 268 |
| MS.gene40456.t1 | MTR_4g006010 | 74.627 | 134 | 31 | 2 | 1 | 134 | 569 | 699 | 3.31e-54 | 180 |
| MS.gene40456.t1 | MTR_8g012320 | 64.444 | 135 | 43 | 4 | 1 | 134 | 572 | 702 | 6.28e-51 | 171 |
| MS.gene40456.t1 | MTR_6g007807 | 68.148 | 135 | 39 | 3 | 1 | 134 | 535 | 666 | 1.34e-40 | 143 |
| MS.gene40456.t1 | MTR_3g103500 | 62.044 | 137 | 42 | 3 | 1 | 134 | 569 | 698 | 4.40e-36 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40456.t1 | AT2G24630 | 74.627 | 134 | 32 | 2 | 1 | 134 | 559 | 690 | 2.45e-51 | 172 |
| MS.gene40456.t1 | AT2G24630 | 74.627 | 134 | 32 | 2 | 1 | 134 | 559 | 690 | 2.45e-51 | 172 |
| MS.gene40456.t1 | AT4G31590 | 77.037 | 135 | 29 | 2 | 1 | 134 | 559 | 692 | 6.11e-48 | 163 |
| MS.gene40456.t1 | AT3G07330 | 61.940 | 134 | 47 | 2 | 1 | 134 | 553 | 682 | 1.24e-46 | 160 |
| MS.gene40456.t1 | AT3G07330 | 61.940 | 134 | 47 | 2 | 1 | 134 | 553 | 682 | 1.24e-46 | 160 |
| MS.gene40456.t1 | AT4G07960 | 61.654 | 133 | 44 | 2 | 1 | 133 | 573 | 698 | 1.13e-42 | 149 |
| MS.gene40456.t1 | AT4G07960 | 61.654 | 133 | 44 | 2 | 1 | 133 | 573 | 698 | 1.13e-42 | 149 |
| MS.gene40456.t1 | AT3G28180 | 59.060 | 149 | 40 | 3 | 1 | 134 | 531 | 673 | 2.46e-41 | 145 |
Find 0 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTTAATGCAATGGTATC+TGG | + | 24207:10044-10063 | MS.gene40456:CDS | 25.0% |
| ! | TGTGACTAAATTTAATGCAA+TGG | + | 24207:10037-10056 | MS.gene40456:CDS | 25.0% |
| !! | ATATAAAAAAGAGCTGACTT+TGG | + | 24207:10283-10302 | MS.gene40456:CDS | 25.0% |
| !! | ATCTTGTTAGCTTTCTTAAC+AGG | - | 24207:10263-10282 | None:intergenic | 30.0% |
| !! | TCTACTTTCTGTTTTTCCAA+GGG | + | 24207:10363-10382 | MS.gene40456:CDS | 30.0% |
| !! | TTCTACTTTCTGTTTTTCCA+AGG | + | 24207:10362-10381 | MS.gene40456:CDS | 30.0% |
| !!! | CTTTTGTTGTTCTATTGACT+CGG | - | 24207:10165-10184 | None:intergenic | 30.0% |
| AATTGGGAAGCTCTTATGAA+TGG | + | 24207:10075-10094 | MS.gene40456:CDS | 35.0% | |
| ATTCATAAGAGCTTCCCAAT+TGG | - | 24207:10076-10095 | None:intergenic | 35.0% | |
| GAACAACAAAAGATTCACAG+AGG | + | 24207:10173-10192 | MS.gene40456:CDS | 35.0% | |
| TGTTGAGTTACAGCAATTGA+AGG | + | 24207:10214-10233 | MS.gene40456:CDS | 35.0% | |
| ! | TTAGCTTTCTTAACAGGTTC+TGG | - | 24207:10257-10276 | None:intergenic | 35.0% |
| AGAAGTCTGTTATCTGCACA+AGG | + | 24207:10332-10351 | MS.gene40456:CDS | 40.0% | |
| CAATCAAATCAAGGCCTACA+AGG | - | 24207:10400-10419 | None:intergenic | 40.0% | |
| GTATCTGGACTCTTCCAATT+GGG | + | 24207:10059-10078 | MS.gene40456:CDS | 40.0% | |
| TCTGCTCTCCAATCAAATCA+AGG | - | 24207:10409-10428 | None:intergenic | 40.0% | |
| TGGATTGTCACAAAGAAAGC+TGG | + | 24207:10095-10114 | MS.gene40456:CDS | 40.0% | |
| TTGTCACAAAGAAAGCTGGA+AGG | + | 24207:10099-10118 | MS.gene40456:CDS | 40.0% | |
| ! | CTTGTAGGCCTTGATTTGAT+TGG | + | 24207:10398-10417 | MS.gene40456:CDS | 40.0% |
| CAAGGAAGAATGTCATCCCT+TGG | - | 24207:10382-10401 | None:intergenic | 45.0% | |
| GGGATGACATTCTTCCTTGT+AGG | + | 24207:10383-10402 | MS.gene40456:CDS | 45.0% | |
| GGTATCTGGACTCTTCCAAT+TGG | + | 24207:10058-10077 | MS.gene40456:CDS | 45.0% | |
| CACAGAGGAGCTTCTGACAG+TGG | + | 24207:10188-10207 | MS.gene40456:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24207 | gene | 10032 | 10436 | 10032 | ID=MS.gene40456 |
| 24207 | mRNA | 10032 | 10436 | 10032 | ID=MS.gene40456.t1;Parent=MS.gene40456 |
| 24207 | exon | 10032 | 10436 | 10032 | ID=MS.gene40456.t1.exon1;Parent=MS.gene40456.t1 |
| 24207 | CDS | 10032 | 10436 | 10032 | ID=cds.MS.gene40456.t1;Parent=MS.gene40456.t1 |
| Gene Sequence |
| Protein sequence |
