AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40542

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40542.t1 MTR_5g004910 92.593 432 25 5 1 427 1 430 0.0 785
MS.gene40542.t1 MTR_5g004910 84.722 432 19 5 1 427 1 390 0.0 718
MS.gene40542.t1 MTR_7g029385 36.889 450 256 9 3 427 85 531 5.44e-87 275
MS.gene40542.t1 MTR_1g095580 31.278 227 138 10 137 352 97 316 1.14e-13 72.0
MS.gene40542.t1 MTR_1g095580 33.495 206 117 11 137 329 97 295 2.65e-13 70.9
MS.gene40542.t1 MTR_1g095580 33.495 206 117 11 137 329 97 295 3.54e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40542.t1 AT3G19440 53.089 437 172 9 16 427 41 469 1.67e-140 410
MS.gene40542.t1 AT3G19440 51.325 302 121 6 16 292 41 341 1.89e-92 283
MS.gene40542.t1 AT1G78910 41.954 348 192 4 89 427 20 366 3.55e-92 283
MS.gene40542.t1 AT1G78910 40.323 372 212 4 65 427 105 475 6.46e-91 283
MS.gene40542.t1 AT1G76050 28.761 226 109 8 137 327 150 358 4.96e-13 70.9

Find 103 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAGTGCTTGAGATATAT+TGG 0.249374 5.2:+278693 None:intergenic
CCACCTTGCACTGGCATTCC+AGG 0.274384 5.2:+280657 None:intergenic
TGCTCCCCTTATTCAAATTA+AGG 0.275216 5.2:+278767 None:intergenic
GTGTTAGGTACACCGATAAT+TGG 0.277960 5.2:-278898 MS.gene40542:CDS
GACGAAAGAGTTTATGGATA+AGG 0.301927 5.2:+280910 None:intergenic
AATTTGTTGTTTAACAGAAT+TGG 0.308662 5.2:+280788 None:intergenic
CTTCGACCCCTGCCCTGAAA+TGG 0.310329 5.2:-280965 MS.gene40542:CDS
AAGGAAGAGGCACTCCCTTT+TGG 0.322488 5.2:-278790 MS.gene40542:CDS
GAAGTGAGAGATTCATGTTT+GGG 0.323404 5.2:+280750 None:intergenic
CGGTATTGGGCATTGGTTCT+TGG 0.326843 5.2:-280336 MS.gene40542:CDS
ATTTGTTGTTTAACAGAATT+GGG 0.355318 5.2:+280789 None:intergenic
ACGAAAGAGTTTATGGATAA+GGG 0.356956 5.2:+280911 None:intergenic
TTTGGCCTTAATTTGAATAA+GGG 0.361194 5.2:-278772 MS.gene40542:CDS
TGTTAGGTACACCGATAATT+GGG 0.365218 5.2:-278897 MS.gene40542:CDS
ATGCCCAATACCGTCTTTGT+AGG 0.365936 5.2:+280346 None:intergenic
TGCAGTGCTTGAGATATATT+GGG 0.367867 5.2:+278694 None:intergenic
AATCCTACAAAGACGGTATT+GGG 0.380224 5.2:-280349 MS.gene40542:CDS
TGGGAGCGGAAGATGGAAAA+TGG 0.387078 5.2:-281040 MS.gene40542:CDS
AGAAGTGAGAGATTCATGTT+TGG 0.393607 5.2:+280749 None:intergenic
AACATGATAACAAGTTCAAA+AGG 0.395586 5.2:-280857 MS.gene40542:CDS
TAAACTCTTTCGTCTAAAAC+AGG 0.401047 5.2:-280901 MS.gene40542:CDS
ATAATTGGGGACTACAAGTA+TGG 0.402405 5.2:-278883 MS.gene40542:CDS
CTTGGATGTCCTAGACGTTC+AGG 0.404141 5.2:-280318 MS.gene40542:CDS
ACAGTTTGATTTGTCTAATC+CGG 0.412494 5.2:-278836 MS.gene40542:CDS
AACATCGTATAACCCATTTC+AGG 0.413289 5.2:+280952 None:intergenic
GAATCCTACAAAGACGGTAT+TGG 0.413483 5.2:-280350 MS.gene40542:CDS
GTCATCTTCGGCGGTGGGAG+CGG 0.414515 5.2:-281054 MS.gene40542:CDS
TGTTATCATGTTGTTGTTGT+GGG 0.414635 5.2:+280868 None:intergenic
TACTTGTAGTCCCCAATTAT+CGG 0.434618 5.2:+278886 None:intergenic
AGACTAGACAGAGATTGTTC+TGG 0.437828 5.2:-280552 MS.gene40542:CDS
CGGAGTCATTAATGCTCCAC+TGG 0.441021 5.2:-280295 MS.gene40542:CDS
TTCGACCCCTGCCCTGAAAT+GGG 0.457533 5.2:-280964 MS.gene40542:CDS
TTGTTATCATGTTGTTGTTG+TGG 0.457895 5.2:+280867 None:intergenic
TCAGTTGTCATCTTCGGCGG+TGG 0.461851 5.2:-281060 MS.gene40542:CDS
CATTAATGCTCCACTGGGTA+AGG 0.462981 5.2:-280289 MS.gene40542:intron
TCGGAGCCGCCGTGAGATCA+TGG 0.463090 5.2:-281094 MS.gene40542:CDS
TTGGAGCTTTCCCCTTTAAC+CGG 0.469543 5.2:-279075 MS.gene40542:CDS
CCTGAGTTCAAAGTATCCTT+AGG 0.470817 5.2:+280828 None:intergenic
TTATTCAAATTAAGGCCAAA+AGG 0.471079 5.2:+278775 None:intergenic
ATATTAATGCCACCTTGCAC+TGG 0.478777 5.2:+280648 None:intergenic
CGTATAACCCATTTCAGGGC+AGG 0.484818 5.2:+280957 None:intergenic
TCGGCGGTGGGAGCGGAAGA+TGG 0.491571 5.2:-281047 MS.gene40542:CDS
AGTAGTTGAATCACTTGAGC+TGG 0.493399 5.2:-278632 MS.gene40542:CDS
ATCTCACGGCGGCTCCGACT+CGG 0.498702 5.2:+281099 None:intergenic
TCGTCGTCAGTTGTCATCTT+CGG 0.501141 5.2:-281066 MS.gene40542:CDS
CTTGTTCTAAACAAACCTCC+TGG 0.510763 5.2:-280675 MS.gene40542:CDS
TATTCAAATTAAGGCCAAAA+GGG 0.513142 5.2:+278776 None:intergenic
ACAAAGACGGTATTGGGCAT+TGG 0.515751 5.2:-280343 MS.gene40542:CDS
GTTCAAAAGGGTTACACCTA+AGG 0.516651 5.2:-280844 MS.gene40542:CDS
TTACTCTCAACCTCCTCGAT+TGG 0.520303 5.2:-280580 MS.gene40542:CDS
TTGTTCTGGAATTCTTGTCA+TGG 0.537926 5.2:-280538 MS.gene40542:CDS
TGTTCTGGAATTCTTGTCAT+GGG 0.542629 5.2:-280537 MS.gene40542:CDS
AACATCAAGGGCTTCAAATG+AGG 0.542967 5.2:-280466 MS.gene40542:intron
TTGGCCTTAATTTGAATAAG+GGG 0.543687 5.2:-278771 MS.gene40542:CDS
TGAATCACTTGAGCTGGAAG+CGG 0.546029 5.2:-278626 MS.gene40542:CDS
CAGTTGTCATCTTCGGCGGT+GGG 0.555838 5.2:-281059 MS.gene40542:CDS
ACATCGTATAACCCATTTCA+GGG 0.562278 5.2:+280953 None:intergenic
CCTAAGGATACTTTGAACTC+AGG 0.564942 5.2:-280828 MS.gene40542:CDS
GTCCACTGTGCTGAAGTGTT+AGG 0.564993 5.2:-278913 MS.gene40542:CDS
TGATGCAGGTTATACATGGT+TGG 0.566674 5.2:-279094 MS.gene40542:intron
CCTTGCACTGGCATTCCAGG+AGG 0.570257 5.2:+280660 None:intergenic
ACATGATAACAAGTTCAAAA+GGG 0.572750 5.2:-280856 MS.gene40542:CDS
GACGGCAAGCTCATAAGAAG+TGG 0.573583 5.2:-278861 MS.gene40542:CDS
GTATAACCCATTTCAGGGCA+GGG 0.573844 5.2:+280958 None:intergenic
TCGAAGTCGAAGAGGTCACT+TGG 0.576852 5.2:+280982 None:intergenic
ATGACTCCGCCTGAACGTCT+AGG 0.583378 5.2:+280309 None:intergenic
AAGGTGGCATTAATATCAAA+CGG 0.584548 5.2:-280641 MS.gene40542:CDS
TATAACCCATTTCAGGGCAG+GGG 0.588867 5.2:+280959 None:intergenic
GTGATTTCATCATCGTCTCA+AGG 0.589230 5.2:-279793 MS.gene40542:intron
GTGAGAGATTCATGTTTGGG+GGG 0.599286 5.2:+280753 None:intergenic
TTGTTTAGAACAAGAATGTG+AGG 0.599711 5.2:+280684 None:intergenic
AGTGAGAGATTCATGTTTGG+GGG 0.602687 5.2:+280752 None:intergenic
CCTCCTGGAATGCCAGTGCA+AGG 0.603092 5.2:-280660 MS.gene40542:CDS
ACACTTCAGCACAGTGGACA+CGG 0.608556 5.2:+278917 None:intergenic
ACGGCAAGCTCATAAGAAGT+GGG 0.609384 5.2:-278860 MS.gene40542:CDS
AAGTGAGAGATTCATGTTTG+GGG 0.615302 5.2:+280751 None:intergenic
GTTTGATTTGTCTAATCCGG+AGG 0.618245 5.2:-278833 MS.gene40542:CDS
AAAAGAGAATCCTACAAAGA+CGG 0.625860 5.2:-280356 MS.gene40542:CDS
TTTCAGGTCGTGGTTGACAA+CGG 0.626018 5.2:-279889 MS.gene40542:intron
TTTAAATAAATAGAATGACA+CGG 0.627054 5.2:-280379 MS.gene40542:intron
TTTACAGTAGTAGTACTCAC+AGG 0.632848 5.2:+281005 None:intergenic
TTGGGGACTACAAGTATGGA+CGG 0.637760 5.2:-278879 MS.gene40542:CDS
GGCAGGGGTCGAAGTCGAAG+AGG 0.644349 5.2:+280974 None:intergenic
GTTAGGTACACCGATAATTG+GGG 0.648978 5.2:-278896 MS.gene40542:CDS
TCTAGTCTATGCACCAATCG+AGG 0.653983 5.2:+280567 None:intergenic
ACAAGTATGTATGTCCGAGT+CGG 0.658753 5.2:-281113 None:intergenic
GGAGTCATTAATGCTCCACT+GGG 0.666010 5.2:-280294 MS.gene40542:CDS
GTAATGATGCAGGTTATACA+TGG 0.670336 5.2:-279098 MS.gene40542:intron
GGATGTCCTAGACGTTCAGG+CGG 0.673625 5.2:-280315 MS.gene40542:CDS
TTTAACCGGTAGAAAACACC+AGG 0.674752 5.2:-279061 MS.gene40542:intron
TCGTCAGTTGTCATCTTCGG+CGG 0.676738 5.2:-281063 MS.gene40542:CDS
GAAGATGGAATGCAAAAGTG+CGG 0.677074 5.2:+280497 None:intergenic
ACGACGACTCCATGATCTCA+CGG 0.680735 5.2:+281085 None:intergenic
CGGCAAGCTCATAAGAAGTG+GGG 0.688690 5.2:-278859 MS.gene40542:CDS
GAGACGATGATGAAATCACT+CGG 0.700343 5.2:+279797 None:intergenic
CCTGGAATGCCAGTGCAAGG+TGG 0.709094 5.2:-280657 MS.gene40542:CDS
CAAGTGATTCAACTACTGAG+AGG 0.710001 5.2:+278638 None:intergenic
TACCTAACACTTCAGCACAG+TGG 0.710893 5.2:+278911 None:intergenic
AGTCTATGCACCAATCGAGG+AGG 0.720434 5.2:+280570 None:intergenic
ATGGATACAACCTTACCCAG+TGG 0.725015 5.2:+280279 None:intergenic
ACAGTAGTAGTACTCACAGG+TGG 0.735705 5.2:+281008 None:intergenic
TTGCAATGAAGATGTAATCG+AGG 0.736658 5.2:+278730 None:intergenic
ACGACTCCATGATCTCACGG+CGG 0.860711 5.2:+281088 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATAAATAGAATGACA+CGG - chr5.2:279307-279326 MS.gene40542:intron 15.0%
!!! TTTAAACTTATCTTTAGTTT+TGG - chr5.2:280076-280095 MS.gene40542:intron 15.0%
!! AACAATCAGAATAGAAATTA+TGG + chr5.2:280731-280750 None:intergenic 20.0%
!! ACAATCAGAATAGAAATTAT+GGG + chr5.2:280730-280749 None:intergenic 20.0%
!!! AATTTGTTGTTTAACAGAAT+TGG + chr5.2:278901-278920 None:intergenic 20.0%
!!! ATTTGTTGTTTAACAGAATT+GGG + chr5.2:278900-278919 None:intergenic 20.0%
!!! CTTCAATTTAATTTCATGTT+TGG - chr5.2:280352-280371 MS.gene40542:CDS 20.0%
!!! TCACAAATTTTCCATATTTT+AGG + chr5.2:280472-280491 None:intergenic 20.0%
! AAAACTAAACATAACAAAGG+GGG + chr5.2:280511-280530 None:intergenic 25.0%
! AAATGTTTGAGCCTAAAATA+TGG - chr5.2:280458-280477 MS.gene40542:intron 25.0%
! AACATGATAACAAGTTCAAA+AGG - chr5.2:278829-278848 MS.gene40542:CDS 25.0%
! AACATTGTGATTTAACTGAA+AGG + chr5.2:279451-279470 None:intergenic 25.0%
! ACAACATTATTGACAATATG+AGG + chr5.2:279758-279777 None:intergenic 25.0%
! ACATGATAACAAGTTCAAAA+GGG - chr5.2:278830-278849 MS.gene40542:CDS 25.0%
! AGCAATAAAGATAATTTGAG+AGG + chr5.2:280102-280121 None:intergenic 25.0%
! AGGAAAACTAAACATAACAA+AGG + chr5.2:280514-280533 None:intergenic 25.0%
! CAATCAGAATAGAAATTATG+GGG + chr5.2:280729-280748 None:intergenic 25.0%
! GAAAACTAAACATAACAAAG+GGG + chr5.2:280512-280531 None:intergenic 25.0%
! GGAAAACTAAACATAACAAA+GGG + chr5.2:280513-280532 None:intergenic 25.0%
! TAAGTCACCTTAAAATTTAG+AGG - chr5.2:279949-279968 MS.gene40542:intron 25.0%
! TAGGATTTAGCAAGATAATT+AGG + chr5.2:279528-279547 None:intergenic 25.0%
! TATTCAAATTAAGGCCAAAA+GGG + chr5.2:280913-280932 None:intergenic 25.0%
! TTATTCAAATTAAGGCCAAA+AGG + chr5.2:280914-280933 None:intergenic 25.0%
!! ACATCAACCTCTAAATTTTA+AGG + chr5.2:279959-279978 None:intergenic 25.0%
!! TAAATCACAATGTTTTGCAA+TGG - chr5.2:279457-279476 MS.gene40542:intron 25.0%
!! TTTTGTAAACTCAACTAGTT+TGG + chr5.2:280234-280253 None:intergenic 25.0%
!!! AATGGTTCTTTATTTTCTCT+TGG - chr5.2:279475-279494 MS.gene40542:intron 25.0%
!!! GGAAAATAGAATTTTAGTCT+GGG - chr5.2:280373-280392 MS.gene40542:intron 25.0%
!!! TGGAAAATAGAATTTTAGTC+TGG - chr5.2:280372-280391 MS.gene40542:intron 25.0%
!!! TTTGGCCTTAATTTGAATAA+GGG - chr5.2:280914-280933 MS.gene40542:CDS 25.0%
!!! TTTTGGCCTTAATTTGAATA+AGG - chr5.2:280913-280932 MS.gene40542:CDS 25.0%
AAAAGAGAATCCTACAAAGA+CGG - chr5.2:279330-279349 MS.gene40542:intron 30.0%
AAACTAAACATAACAAAGGG+GGG + chr5.2:280510-280529 None:intergenic 30.0%
AAGGAAACATAGGGTAAAAT+AGG + chr5.2:279547-279566 None:intergenic 30.0%
AAGGTGGCATTAATATCAAA+CGG - chr5.2:279045-279064 MS.gene40542:intron 30.0%
ACGAAAGAGTTTATGGATAA+GGG + chr5.2:278778-278797 None:intergenic 30.0%
CAATATGCAATAGTCAGTAA+AGG - chr5.2:279668-279687 MS.gene40542:intron 30.0%
CTGAAACCAAATCAAATGAA+GGG + chr5.2:279783-279802 None:intergenic 30.0%
GAAACTAATGTAAAGCAGAA+AGG + chr5.2:279566-279585 None:intergenic 30.0%
TAAAGCAGAAAGGAAACATA+GGG + chr5.2:279556-279575 None:intergenic 30.0%
TGTTGTGATAGAAATCATGT+AGG + chr5.2:280427-280446 None:intergenic 30.0%
TTCACATCATCACATGTTAT+TGG - chr5.2:280283-280302 MS.gene40542:intron 30.0%
TTGTAACCCTTCATTTGATT+TGG - chr5.2:279774-279793 MS.gene40542:intron 30.0%
TTGTATCCATATTCTCAACT+TGG - chr5.2:279420-279439 MS.gene40542:intron 30.0%
! AGTTTATGGATAAGGGTTTT+AGG + chr5.2:278771-278790 None:intergenic 30.0%
! TAAACTCTTTCGTCTAAAAC+AGG - chr5.2:278785-278804 MS.gene40542:CDS 30.0%
! TGAACTTGTGTTTTATAGCA+CGG - chr5.2:280004-280023 MS.gene40542:intron 30.0%
! TGTTATCATGTTGTTGTTGT+GGG + chr5.2:278821-278840 None:intergenic 30.0%
! TTGTTATCATGTTGTTGTTG+TGG + chr5.2:278822-278841 None:intergenic 30.0%
! TTGTTTAGAACAAGAATGTG+AGG + chr5.2:279005-279024 None:intergenic 30.0%
!! ACAGTTTGATTTGTCTAATC+CGG - chr5.2:280850-280869 MS.gene40542:CDS 30.0%
!! GTTTTAGACGAAAGAGTTTA+TGG + chr5.2:278785-278804 None:intergenic 30.0%
!! TTGGCCTTAATTTGAATAAG+GGG - chr5.2:280915-280934 MS.gene40542:CDS 30.0%
!!! AGCATGCATTTTTTGCATTT+CGG + chr5.2:280210-280229 None:intergenic 30.0%
AACACCAGGTAATAGAAACA+AGG - chr5.2:280639-280658 MS.gene40542:CDS 35.0%
AACATCGTATAACCCATTTC+AGG + chr5.2:278737-278756 None:intergenic 35.0%
AAGTGAGAGATTCATGTTTG+GGG + chr5.2:278938-278957 None:intergenic 35.0%
AATCCTACAAAGACGGTATT+GGG - chr5.2:279337-279356 MS.gene40542:intron 35.0%
AATTAAGATCTCCCTCCTTA+TGG - chr5.2:280179-280198 MS.gene40542:intron 35.0%
ACACCAGGTAATAGAAACAA+GGG - chr5.2:280640-280659 MS.gene40542:CDS 35.0%
ACATCGTATAACCCATTTCA+GGG + chr5.2:278736-278755 None:intergenic 35.0%
AGAAGTGAGAGATTCATGTT+TGG + chr5.2:278940-278959 None:intergenic 35.0%
AGGCTCAAACATTTGCAATT+AGG + chr5.2:280452-280471 None:intergenic 35.0%
ATAATTGGGGACTACAAGTA+TGG - chr5.2:280803-280822 MS.gene40542:CDS 35.0%
ATACATGAAAAAGAGTTGGG+AGG - chr5.2:281093-281112 MS.gene40542:CDS 35.0%
ATTTCGGTAATTCCATAAGG+AGG + chr5.2:280194-280213 None:intergenic 35.0%
CCCATACATGAAAAAGAGTT+GGG - chr5.2:281090-281109 MS.gene40542:CDS 35.0%
CCTGAAACCAAATCAAATGA+AGG + chr5.2:279784-279803 None:intergenic 35.0%
CTCAACTGAAAAACATCTCA+AGG - chr5.2:280877-280896 MS.gene40542:CDS 35.0%
CTTCCGTGAAAAAACATCAA+GGG - chr5.2:279208-279227 MS.gene40542:intron 35.0%
GAAGTGAGAGATTCATGTTT+GGG + chr5.2:278939-278958 None:intergenic 35.0%
GACGAAAGAGTTTATGGATA+AGG + chr5.2:278779-278798 None:intergenic 35.0%
GCACAAGTAATTAAACCGAT+AGG - chr5.2:280395-280414 MS.gene40542:intron 35.0%
GTAAAGCAGAAAGGAAACAT+AGG + chr5.2:279557-279576 None:intergenic 35.0%
GTAATGATGCAGGTTATACA+TGG - chr5.2:280588-280607 MS.gene40542:CDS 35.0%
TACTTGTAGTCCCCAATTAT+CGG + chr5.2:280803-280822 None:intergenic 35.0%
TATTGCATATTGCAGCTGTA+AGG + chr5.2:279660-279679 None:intergenic 35.0%
TCTATTCTGATTGTTTGTGC+TGG - chr5.2:280736-280755 MS.gene40542:CDS 35.0%
TCTCCTTTGATCTTCTTAGT+TGG - chr5.2:280544-280563 MS.gene40542:CDS 35.0%
TCTTCCGTGAAAAAACATCA+AGG - chr5.2:279207-279226 MS.gene40542:intron 35.0%
TGAAAAACATCTCAAGGAAG+AGG - chr5.2:280883-280902 MS.gene40542:CDS 35.0%
TGCAGTGCTTGAGATATATT+GGG + chr5.2:280995-281014 None:intergenic 35.0%
TGCATTTCGGTAATTCCATA+AGG + chr5.2:280197-280216 None:intergenic 35.0%
TGCTCCCCTTATTCAAATTA+AGG + chr5.2:280922-280941 None:intergenic 35.0%
TGTTAGGTACACCGATAATT+GGG - chr5.2:280789-280808 MS.gene40542:CDS 35.0%
TTGCAATGAAGATGTAATCG+AGG + chr5.2:280959-280978 None:intergenic 35.0%
TTGCAGTGCTTGAGATATAT+TGG + chr5.2:280996-281015 None:intergenic 35.0%
TTGCCAACTAAGAAGATCAA+AGG + chr5.2:280550-280569 None:intergenic 35.0%
TTTACAGTAGTAGTACTCAC+AGG + chr5.2:278684-278703 None:intergenic 35.0%
TTTATAGCACGGCATAATTC+TGG - chr5.2:280015-280034 MS.gene40542:intron 35.0%
TTTCGGTAATTCCATAAGGA+GGG + chr5.2:280193-280212 None:intergenic 35.0%
! ACATTAGTTTCCATAGTGTG+TGG - chr5.2:279575-279594 MS.gene40542:intron 35.0%
! GACAATATGAGGAGCTTTTA+TGG + chr5.2:279747-279766 None:intergenic 35.0%
! GATTAGCCAAGTTGAGAATA+TGG + chr5.2:279429-279448 None:intergenic 35.0%
! TATTACCTGGTGTTTTCTAC+CGG + chr5.2:280633-280652 None:intergenic 35.0%
!! CAACTCTTTTTCATGTATGG+GGG + chr5.2:281091-281110 None:intergenic 35.0%
!! CCAACTCTTTTTCATGTATG+GGG + chr5.2:281092-281111 None:intergenic 35.0%
!! CCCAACTCTTTTTCATGTAT+GGG + chr5.2:281093-281112 None:intergenic 35.0%
!! CCTTCATTTGATTTGGTTTC+AGG - chr5.2:279781-279800 MS.gene40542:intron 35.0%
!! TCCCAACTCTTTTTCATGTA+TGG + chr5.2:281094-281113 None:intergenic 35.0%
!! TGTTCTGGAATTCTTGTCAT+GGG - chr5.2:279149-279168 MS.gene40542:intron 35.0%
!! TTGTTCTGGAATTCTTGTCA+TGG - chr5.2:279148-279167 MS.gene40542:intron 35.0%
!!! AAGCCCTTGATGTTTTTTCA+CGG + chr5.2:279214-279233 None:intergenic 35.0%
!!! ATAAGGGTTTTAGGTAGTTG+AGG + chr5.2:278762-278781 None:intergenic 35.0%
!!! TAAGGGTTTTAGGTAGTTGA+GGG + chr5.2:278761-278780 None:intergenic 35.0%
!!! TGATGTTTTTTCACGGAAGA+TGG + chr5.2:279207-279226 None:intergenic 35.0%
AACATCAAGGGCTTCAAATG+AGG - chr5.2:279220-279239 MS.gene40542:intron 40.0%
AATGTCACAGACACTGCAAA+AGG + chr5.2:280057-280076 None:intergenic 40.0%
AGACTAGACAGAGATTGTTC+TGG - chr5.2:279134-279153 MS.gene40542:intron 40.0%
AGTAGTTGAATCACTTGAGC+TGG - chr5.2:281054-281073 MS.gene40542:CDS 40.0%
AGTGAGAGATTCATGTTTGG+GGG + chr5.2:278937-278956 None:intergenic 40.0%
ATATTAATGCCACCTTGCAC+TGG + chr5.2:279041-279060 None:intergenic 40.0%
ATGTCACAGACACTGCAAAA+GGG + chr5.2:280056-280075 None:intergenic 40.0%
CAAGTGATTCAACTACTGAG+AGG + chr5.2:281051-281070 None:intergenic 40.0%
CCCCATACATGAAAAAGAGT+TGG - chr5.2:281089-281108 MS.gene40542:CDS 40.0%
CCTAAGGATACTTTGAACTC+AGG - chr5.2:278858-278877 MS.gene40542:CDS 40.0%
CGAGCTGATAGCTACATAAA+AGG - chr5.2:280324-280343 MS.gene40542:CDS 40.0%
CTGATAGCTACATAAAAGGC+AGG - chr5.2:280328-280347 MS.gene40542:CDS 40.0%
GAAGATGGAATGCAAAAGTG+CGG + chr5.2:279192-279211 None:intergenic 40.0%
GAATCATTACCAGCTGATCT+TGG - chr5.2:280665-280684 MS.gene40542:CDS 40.0%
GAATCCTACAAAGACGGTAT+TGG - chr5.2:279336-279355 MS.gene40542:intron 40.0%
GAGACGATGATGAAATCACT+CGG + chr5.2:279892-279911 None:intergenic 40.0%
GTGATTTCATCATCGTCTCA+AGG - chr5.2:279893-279912 MS.gene40542:intron 40.0%
GTGTTAGGTACACCGATAAT+TGG - chr5.2:280788-280807 MS.gene40542:CDS 40.0%
GTTAGGTACACCGATAATTG+GGG - chr5.2:280790-280809 MS.gene40542:CDS 40.0%
GTTCAAAAGGGTTACACCTA+AGG - chr5.2:278842-278861 MS.gene40542:CDS 40.0%
TAAACATAACAAAGGGGGGA+GGG + chr5.2:280506-280525 None:intergenic 40.0%
TCATGTAGGACATCACCTAT+CGG + chr5.2:280413-280432 None:intergenic 40.0%
TCATTACCAGTGACATGAGT+CGG + chr5.2:280575-280594 None:intergenic 40.0%
TGATGCAGGTTATACATGGT+TGG - chr5.2:280592-280611 MS.gene40542:CDS 40.0%
TGGCAATCATTGTGTATACG+AGG + chr5.2:279601-279620 None:intergenic 40.0%
TTTAACCGGTAGAAAACACC+AGG - chr5.2:280625-280644 MS.gene40542:CDS 40.0%
TTTCATCATCGTCTCAAGGT+TGG - chr5.2:279897-279916 MS.gene40542:intron 40.0%
! CCTGAGTTCAAAGTATCCTT+AGG + chr5.2:278861-278880 None:intergenic 40.0%
! CTTGTTCTAAACAAACCTCC+TGG - chr5.2:279011-279030 MS.gene40542:intron 40.0%
! TCAAAGGAGACACGTTTTGA+AGG + chr5.2:280534-280553 None:intergenic 40.0%
!! CTTCCCTTGTTTCTATTACC+TGG + chr5.2:280646-280665 None:intergenic 40.0%
!! GGTGTTTTCTACCGGTTAAA+GGG + chr5.2:280625-280644 None:intergenic 40.0%
!! GTGTTTTCTACCGGTTAAAG+GGG + chr5.2:280624-280643 None:intergenic 40.0%
!! GTTTGATTTGTCTAATCCGG+AGG - chr5.2:280853-280872 MS.gene40542:CDS 40.0%
!! TGGTGTTTTCTACCGGTTAA+AGG + chr5.2:280626-280645 None:intergenic 40.0%
!! TGTTTTTCAGTTGAGTCCTC+CGG + chr5.2:280872-280891 None:intergenic 40.0%
!! TTTGATTTGGTTTCAGGTCG+TGG - chr5.2:279787-279806 MS.gene40542:intron 40.0%
AAACATAACAAAGGGGGGAG+GGG + chr5.2:280505-280524 None:intergenic 45.0%
ACAAGTTCACCCACACTTAC+AGG + chr5.2:279993-280012 None:intergenic 45.0%
ACAGTAGTAGTACTCACAGG+TGG + chr5.2:278681-278700 None:intergenic 45.0%
ACGGCAAGCTCATAAGAAGT+GGG - chr5.2:280826-280845 MS.gene40542:CDS 45.0%
ATGCCCAATACCGTCTTTGT+AGG + chr5.2:279343-279362 None:intergenic 45.0%
ATGGATACAACCTTACCCAG+TGG + chr5.2:279410-279429 None:intergenic 45.0%
ATGTGACAGCAGAGAGTTGT+TGG + chr5.2:279621-279640 None:intergenic 45.0%
CAAGTTCACCCACACTTACA+GGG + chr5.2:279992-280011 None:intergenic 45.0%
CATTAATGCTCCACTGGGTA+AGG - chr5.2:279397-279416 MS.gene40542:intron 45.0%
CATTACCAGTGACATGAGTC+GGG + chr5.2:280574-280593 None:intergenic 45.0%
CTAAACATAACAAAGGGGGG+AGG + chr5.2:280507-280526 None:intergenic 45.0%
CTGCTGTCACATGAATGCTT+AGG - chr5.2:279629-279648 MS.gene40542:intron 45.0%
GGAGTCATTAATGCTCCACT+GGG - chr5.2:279392-279411 MS.gene40542:intron 45.0%
GTATAACCCATTTCAGGGCA+GGG + chr5.2:278731-278750 None:intergenic 45.0%
GTCACATGAATGCTTAGGCT+AGG - chr5.2:279634-279653 MS.gene40542:intron 45.0%
GTGAGAGATTCATGTTTGGG+GGG + chr5.2:278936-278955 None:intergenic 45.0%
GTGATTGTCCCTGTAAGTGT+GGG - chr5.2:279981-280000 MS.gene40542:intron 45.0%
GTTGTAGTACCAAGATCAGC+TGG + chr5.2:280677-280696 None:intergenic 45.0%
TACCTAACACTTCAGCACAG+TGG + chr5.2:280778-280797 None:intergenic 45.0%
TATAACCCATTTCAGGGCAG+GGG + chr5.2:278730-278749 None:intergenic 45.0%
TCGTCGTCAGTTGTCATCTT+CGG - chr5.2:278620-278639 MS.gene40542:CDS 45.0%
TCTAGTCTATGCACCAATCG+AGG + chr5.2:279122-279141 None:intergenic 45.0%
TGAATCACTTGAGCTGGAAG+CGG - chr5.2:281060-281079 MS.gene40542:CDS 45.0%
TGTGATTGTCCCTGTAAGTG+TGG - chr5.2:279980-279999 MS.gene40542:intron 45.0%
TTACTCTCAACCTCCTCGAT+TGG - chr5.2:279106-279125 MS.gene40542:intron 45.0%
TTGGAGCTTTCCCCTTTAAC+CGG - chr5.2:280611-280630 MS.gene40542:CDS 45.0%
TTGGGGACTACAAGTATGGA+CGG - chr5.2:280807-280826 MS.gene40542:CDS 45.0%
! ACAAAGACGGTATTGGGCAT+TGG - chr5.2:279343-279362 MS.gene40542:intron 45.0%
! CATGTCACTGGTAATGATGC+AGG - chr5.2:280578-280597 MS.gene40542:CDS 45.0%
! TTTCAGGTCGTGGTTGACAA+CGG - chr5.2:279797-279816 MS.gene40542:CDS 45.0%
AACATAACAAAGGGGGGAGG+GGG + chr5.2:280504-280523 None:intergenic 50.0%
ACACTTCAGCACAGTGGACA+CGG + chr5.2:280772-280791 None:intergenic 50.0%
ACGACGACTCCATGATCTCA+CGG + chr5.2:278604-278623 None:intergenic 50.0%
AGTCTATGCACCAATCGAGG+AGG + chr5.2:279119-279138 None:intergenic 50.0%
CGGAGTCATTAATGCTCCAC+TGG - chr5.2:279391-279410 MS.gene40542:intron 50.0%
CGGCAAGCTCATAAGAAGTG+GGG - chr5.2:280827-280846 MS.gene40542:CDS 50.0%
CGTATAACCCATTTCAGGGC+AGG + chr5.2:278732-278751 None:intergenic 50.0%
CTTGGATGTCCTAGACGTTC+AGG - chr5.2:279368-279387 MS.gene40542:intron 50.0%
GACGGCAAGCTCATAAGAAG+TGG - chr5.2:280825-280844 MS.gene40542:CDS 50.0%
GTATACGAGGCCACACACTA+TGG + chr5.2:279588-279607 None:intergenic 50.0%
TCGAAGTCGAAGAGGTCACT+TGG + chr5.2:278707-278726 None:intergenic 50.0%
TCGTCAGTTGTCATCTTCGG+CGG - chr5.2:278623-278642 MS.gene40542:CDS 50.0%
TGGGAGCGGAAGATGGAAAA+TGG - chr5.2:278646-278665 MS.gene40542:CDS 50.0%
! GTCCACTGTGCTGAAGTGTT+AGG - chr5.2:280773-280792 MS.gene40542:CDS 50.0%
! TCATGTTTGGGGGGTGTAGT+AGG + chr5.2:278927-278946 None:intergenic 50.0%
!! CGGTATTGGGCATTGGTTCT+TGG - chr5.2:279350-279369 MS.gene40542:intron 50.0%
!!! AAGGAAGAGGCACTCCCTTT+TGG - chr5.2:280896-280915 MS.gene40542:CDS 50.0%
ACATAACAAAGGGGGGAGGG+GGG + chr5.2:280503-280522 None:intergenic 55.0%
ACGACTCCATGATCTCACGG+CGG + chr5.2:278601-278620 None:intergenic 55.0%
ATGACTCCGCCTGAACGTCT+AGG + chr5.2:279380-279399 None:intergenic 55.0%
CAGTTGTCATCTTCGGCGGT+GGG - chr5.2:278627-278646 MS.gene40542:CDS 55.0%
GCAATCCCGACTCATGTCAC+TGG - chr5.2:280566-280585 MS.gene40542:CDS 55.0%
GGATGTCCTAGACGTTCAGG+CGG - chr5.2:279371-279390 MS.gene40542:intron 55.0%
TCAGTTGTCATCTTCGGCGG+TGG - chr5.2:278626-278645 MS.gene40542:CDS 55.0%
TTCGACCCCTGCCCTGAAAT+GGG - chr5.2:278722-278741 MS.gene40542:CDS 55.0%
CCTCCTGGAATGCCAGTGCA+AGG - chr5.2:279026-279045 MS.gene40542:intron 60.0%
CCTGGAATGCCAGTGCAAGG+TGG - chr5.2:279029-279048 MS.gene40542:intron 60.0%
CTTCGACCCCTGCCCTGAAA+TGG - chr5.2:278721-278740 MS.gene40542:CDS 60.0%
! CCACCTTGCACTGGCATTCC+AGG + chr5.2:279032-279051 None:intergenic 60.0%
! CCTTGCACTGGCATTCCAGG+AGG + chr5.2:279029-279048 None:intergenic 60.0%
ATCTCACGGCGGCTCCGACT+CGG + chr5.2:278590-278609 None:intergenic 65.0%
GGCAGGGGTCGAAGTCGAAG+AGG + chr5.2:278715-278734 None:intergenic 65.0%
GTCATCTTCGGCGGTGGGAG+CGG - chr5.2:278632-278651 MS.gene40542:CDS 65.0%
TAACAAAGGGGGGAGGGGGG+AGG + chr5.2:280500-280519 None:intergenic 65.0%
TCGGAGCCGCCGTGAGATCA+TGG - chr5.2:278592-278611 MS.gene40542:CDS 65.0%
TCGGCGGTGGGAGCGGAAGA+TGG - chr5.2:278639-278658 MS.gene40542:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 278579 281129 278579 ID=MS.gene40542
chr5.2 mRNA 278579 281129 278579 ID=MS.gene40542.t1;Parent=MS.gene40542
chr5.2 exon 280467 281129 280467 ID=MS.gene40542.t1.exon1;Parent=MS.gene40542.t1
chr5.2 CDS 280467 281129 280467 ID=cds.MS.gene40542.t1;Parent=MS.gene40542.t1
chr5.2 exon 280290 280388 280290 ID=MS.gene40542.t1.exon2;Parent=MS.gene40542.t1
chr5.2 CDS 280290 280388 280290 ID=cds.MS.gene40542.t1;Parent=MS.gene40542.t1
chr5.2 exon 279794 279905 279794 ID=MS.gene40542.t1.exon3;Parent=MS.gene40542.t1
chr5.2 CDS 279794 279905 279794 ID=cds.MS.gene40542.t1;Parent=MS.gene40542.t1
chr5.2 exon 279062 279108 279062 ID=MS.gene40542.t1.exon4;Parent=MS.gene40542.t1
chr5.2 CDS 279062 279108 279062 ID=cds.MS.gene40542.t1;Parent=MS.gene40542.t1
chr5.2 exon 278579 278941 278579 ID=MS.gene40542.t1.exon5;Parent=MS.gene40542.t1
chr5.2 CDS 278579 278941 278579 ID=cds.MS.gene40542.t1;Parent=MS.gene40542.t1
Gene Sequence

>MS.gene40542

ATGTATGTCCGAGTCGGAGCCGCCGTGAGATCATGGAGTCGTCGTCGTCAGTTGTCATCTTCGGCGGTGGGAGCGGAAGATGGAAAATGGCTTACACTGCCACCTGTGAGTACTACTACTGTAAACCAAGTGACCTCTTCGACTTCGACCCCTGCCCTGAAATGGGTTATACGATGTTGCCCTCAACTACCTAAAACCCTTATCCATAAACTCTTTCGTCTAAAACAGGTTCGAATGCTCCCACAACAACAACATGATAACAAGTTCAAAAGGGTTACACCTAAGGATACTTTGAACTCAGGAGATCGTATATTTCTTCCCAATTCTGTTAAACAACAAATTACTCCTACTACACCCCCCAAACATGAATCTCTCACTTCTAAACAAATAAACTTCATTCGTTCTCTTGTTATCTATAAAGATCCTCACATTCTTGTTCTAAACAAACCTCCTGGAATGCCAGTGCAAGGTGGCATTAATATCAAACGGAGTTTAGATGCTGTTGCTGCTGAATCTTTAAATTTTGATTACTCTCAACCTCCTCGATTGGTGCATAGACTAGACAGAGATTGTTCTGGAATTCTTGTCATGGGAAGAGCAAAGACAAGCACCGCACTTTTGCATTCCATCTTCCGTGAAAAAACATCAAGGGCTTCAAATGAGGTAATATTAATAATCACAACTTCAACTTCAATTTGTTTCATATTTTAACTTCATCTTTTATTTTATTTAAATAAATAGAATGACACGGAAAAGAGAATCCTACAAAGACGGTATTGGGCATTGGTTCTTGGATGTCCTAGACGTTCAGGCGGAGTCATTAATGCTCCACTGGGTAAGGTTGTATCCATATTCTCAACTTGGCTAATCCTTTCAGTTAAATCACAATGTTTTGCAATGGTTCTTTATTTTCTCTTGGAACTAAACTCTCTTAACATTTCAAACACCTAATTATCTTGCTAAATCCTATTTTACCCTATGTTTCCTTTCTGCTTTACATTAGTTTCCATAGTGTGTGGCCTCGTATACACAATGATTGCCAACAACTCTCTGCTGTCACATGAATGCTTAGGCTAGGCCTTACAGCTGCAATATGCAATAGTCAGTAAAGGCTTAATCTTTATTAGCTTCTTGTACTTAAGTAATTGATAATTGATTTAATGAGCCATAAAAGCTCCTCATATTGTCAATAATGTTGTAACCCTTCATTTGATTTGGTTTCAGGTCGTGGTTGACAACGGAAAATCTGATCGAATAACTATCATTGACAATTCTGCATTACTGTCATCGCAACATGCAGTTACAGAGTACCGAGTGATTTCATCATCGTCTCAAGGTTGGTATTGAAGTTTACGTTAAATTCTGAGTTTTAAGTCACCTTAAAATTTAGAGGTTGATGTTTGTGATTGTCCCTGTAAGTGTGGGTGAACTTGTGTTTTATAGCACGGCATAATTCTGGCTTAAGATTCTTTTTCCCTTTTGCAGTGTCTGTGACATTTTAAACTTATCTTTAGTTTTGGCCTCTCAAATTATCTTTATTGCTAATGACTAATGACTAATGAGTTAATATAAAAATATCATTAGTGATTTAAGAAACAATAATTAAGATCTCCCTCCTTATGGAATTACCGAAATGCAAAAAATGCATGCTTCCAAACTAGTTGAGTTTACAAAATGATACATTACATAATTTAGTTTAATAATTTCACATCATCACATGTTATTGGATAAAGCAGACACAAAAACGAGCTGATAGCTACATAAAAGGCAGGACTTCAATTTAATTTCATGTTTGGAAAATAGAATTTTAGTCTGGGCACAAGTAATTAAACCGATAGGTGATGTCCTACATGATTTCTATCACAACAGACCTAATTGCAAATGTTTGAGCCTAAAATATGGAAAATTTGTGATGCGTCCTCCCCCCTCCCCCCTTTGTTATGTTTAGTTTTCCTTCAAAACGTGTCTCCTTTGATCTTCTTAGTTGGCAATCCCGACTCATGTCACTGGTAATGATGCAGGTTATACATGGTTGGAGCTTTCCCCTTTAACCGGTAGAAAACACCAGGTAATAGAAACAAGGGAAGAATCATTACCAGCTGATCTTGGTACTACAACTTAGTGTTCAATCAATGCATATGCTGCATCCCCATAATTTCTATTCTGATTGTTTGTGCTGGTTCTGCAGCTCCGTGTCCACTGTGCTGAAGTGTTAGGTACACCGATAATTGGGGACTACAAGTATGGACGGCAAGCTCATAAGAAGTGGGGACAGTTTGATTTGTCTAATCCGGAGGACTCAACTGAAAAACATCTCAAGGAAGAGGCACTCCCTTTTGGCCTTAATTTGAATAAGGGGAGCATCTCTGACAAACATCCTCGATTACATCTTCATTGCAAGCAAATAGTCTTGCCCAATATATCTCAAGCACTGCAAAATGTGCAATCAGTATCAGATTCAAGTTATGACCTCTCAGTAGTTGAATCACTTGAGCTGGAAGCGGATTTGCCCCCATACATGAAAAAGAGTTGGGAGGTGACAAATTCTTGA
Protein sequence

>MS.gene40542.t1

MYVRVGAAVRSWSRRRQLSSSAVGAEDGKWLTLPPVSTTTVNQVTSSTSTPALKWVIRCCPQLPKTLIHKLFRLKQVRMLPQQQHDNKFKRVTPKDTLNSGDRIFLPNSVKQQITPTTPPKHESLTSKQINFIRSLVIYKDPHILVLNKPPGMPVQGGINIKRSLDAVAAESLNFDYSQPPRLVHRLDRDCSGILVMGRAKTSTALLHSIFREKTSRASNENDTEKRILQRRYWALVLGCPRRSGGVINAPLGKVVVDNGKSDRITIIDNSALLSSQHAVTEYRVISSSSQGYTWLELSPLTGRKHQLRVHCAEVLGTPIIGDYKYGRQAHKKWGQFDLSNPEDSTEKHLKEEALPFGLNLNKGSISDKHPRLHLHCKQIVLPNISQALQNVQSVSDSSYDLSVVESLELEADLPPYMKKSWEVTNS