AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40543

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40543.t1 MTR_5g004880 95.120 1127 51 2 1 1124 63 1188 0.0 2170
MS.gene40543.t1 MTR_5g004880 95.031 1127 51 3 1 1124 63 1187 0.0 2164
MS.gene40543.t1 MTR_7g093560 26.766 269 175 7 786 1046 477 731 3.41e-18 90.9
MS.gene40543.t1 MTR_3g101230 25.316 395 236 18 756 1119 618 984 3.41e-12 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40543.t1 AT3G43610 42.698 1164 590 21 1 1114 60 1196 0.0 803
MS.gene40543.t1 AT3G43610 42.661 1165 590 22 1 1114 60 1197 0.0 799
MS.gene40543.t1 AT3G43610 42.660 1158 562 23 1 1114 60 1159 0.0 787
MS.gene40543.t1 AT3G53760 24.789 355 211 10 800 1119 401 734 1.67e-19 95.1
MS.gene40543.t1 AT5G06680 25.651 269 178 6 786 1046 483 737 2.27e-18 91.7
MS.gene40543.t1 AT3G53760 25.000 300 185 8 847 1119 420 706 4.02e-18 90.5
MS.gene40543.t1 AT3G53760 25.362 276 164 6 800 1044 401 665 4.32e-16 84.0

Find 211 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGCATCAAAACCATATT+TGG 0.106834 5.2:+269321 None:intergenic
TCAAGTAGGCCTTGAATTTC+TGG 0.126180 5.2:+267676 None:intergenic
CTGTTAAAACATATTGTTTA+TGG 0.133412 5.2:+269284 None:intergenic
ACTTGATATCATTCCCTTAT+TGG 0.140448 5.2:+269927 None:intergenic
GAACCTAGGCATGTGAATTT+TGG 0.157976 5.2:-263650 MS.gene40543:CDS
TTGCTCCTCAGAGCTGTTTA+AGG 0.160336 5.2:+270143 None:intergenic
TGCAATGTGCACTTGATTTC+CGG 0.193614 5.2:-264200 MS.gene40543:CDS
TCTCAGGGGATCTAATAATT+TGG 0.197182 5.2:-270238 MS.gene40543:intron
TTCAGCATTTAGACCCTTAT+TGG 0.231770 5.2:+266577 None:intergenic
ACATGAAATATGTGATAAAA+TGG 0.256132 5.2:-269977 MS.gene40543:CDS
ATTTGTTCATCTTGTCTCTT+TGG 0.256357 5.2:-267851 MS.gene40543:CDS
CGAGATGGAGTTCCCATTCC+TGG 0.258609 5.2:-271376 MS.gene40543:CDS
AATAATGATTATGGAGAAAT+TGG 0.260319 5.2:-269376 MS.gene40543:CDS
TTCTTTCGATTTCTTAGGAC+TGG 0.266795 5.2:-267376 MS.gene40543:CDS
TGATGTGTGGTGCTCATTAA+AGG 0.279849 5.2:-267241 MS.gene40543:intron
TAACAGCAACGCGACATAAC+TGG 0.285184 5.2:+269722 None:intergenic
TCTTCTGTTGGAGAGATAAT+AGG 0.285491 5.2:+270449 None:intergenic
GGTAATCAGAATAATGATTA+TGG 0.292151 5.2:-269385 MS.gene40543:CDS
ACGTCCCATCTGCAAGTATC+AGG 0.297658 5.2:-271507 MS.gene40543:CDS
CACATCAGTCAATGAAAATA+AGG 0.300804 5.2:+267257 None:intergenic
CAAAGATACTATAGATAATA+TGG 0.301667 5.2:-271173 MS.gene40543:CDS
CTTGCTCACAAGAAGACTTC+TGG 0.303104 5.2:+267644 None:intergenic
TATGGCACATATAACCAATA+AGG 0.304666 5.2:-266591 MS.gene40543:CDS
GGACCCTTGTAAGGTTTATA+TGG 0.312505 5.2:-269479 MS.gene40543:CDS
GTTCGTTCTTTCGATTTCTT+AGG 0.315604 5.2:-267381 MS.gene40543:CDS
TTTGATGAAGTCCTCCAATA+AGG 0.317174 5.2:-269941 MS.gene40543:CDS
GAGCAGCTGGGGAAGACTGC+TGG 0.329494 5.2:-269248 MS.gene40543:CDS
TTCAATAATAAACTCCTTGT+AGG 0.345407 5.2:+271582 None:intergenic
AAGAAGAAAAGAAGGGAGAT+AGG 0.351878 5.2:+272304 None:intergenic
TCTTCATTCCCTTCAGCATA+AGG 0.366390 5.2:-266188 MS.gene40543:intron
ATACATCTGGTGGGAGCAGC+TGG 0.366570 5.2:-269261 MS.gene40543:CDS
GCTGCAAAGTAGATACAAAA+TGG 0.368889 5.2:+266254 None:intergenic
GAAGCCGGCGTATTATTAAC+AGG 0.369704 5.2:+269661 None:intergenic
GATAGCTGTGCATCTACTTA+TGG 0.373668 5.2:-269406 MS.gene40543:CDS
AAAAGAAGGGAGATAGGTTA+TGG 0.375455 5.2:+272310 None:intergenic
TTGGTTATGAATGCAATGTT+AGG 0.377620 5.2:-272357 None:intergenic
GATAGCGATGATGGATGATT+TGG 0.382179 5.2:+272332 None:intergenic
TTCCGGTCTTGTCTTACAAT+AGG 0.387539 5.2:-264183 MS.gene40543:CDS
TGGATTTCTCAAGGACTCAT+TGG 0.388297 5.2:-271356 MS.gene40543:CDS
ACATAACTGGGAATATTTCA+TGG 0.388445 5.2:+269735 None:intergenic
AACATTGGGTTCATACTAAA+GGG 0.392562 5.2:+269577 None:intergenic
TAGATACAAAATGGCTGATC+TGG 0.400262 5.2:+266263 None:intergenic
CAAGAGAAAGCTACTATAGA+AGG 0.400425 5.2:-271144 MS.gene40543:CDS
ATTGTGGGTTTATTAGATCT+TGG 0.402296 5.2:-271630 MS.gene40543:CDS
GAAGCATCAAAACCATATTT+GGG 0.404273 5.2:+269322 None:intergenic
GGGAATATTTCATGGATTGC+AGG 0.408006 5.2:+269743 None:intergenic
ACACCACTTTCCTTCATCTC+TGG 0.408695 5.2:-272272 MS.gene40543:CDS
CATGGATAAATCAAGAGGTT+TGG 0.410907 5.2:+272023 None:intergenic
TACTGTAGAGGACCCTTGTA+AGG 0.417624 5.2:-269488 MS.gene40543:CDS
AAACATTTCTAGTCAACATT+GGG 0.419183 5.2:+269563 None:intergenic
CTTGAGTACCACGAATTCTC+AGG 0.421661 5.2:+269883 None:intergenic
TGAGAGAAGCGTGGATAGTG+TGG 0.422341 5.2:+272064 None:intergenic
AAACAGCTCTGAGGAGCAAT+TGG 0.423495 5.2:-270139 MS.gene40543:CDS
TTGGTTGATGAACCCAAATA+TGG 0.426072 5.2:-269334 MS.gene40543:CDS
TCATTATCAAAAGAAGAAAC+TGG 0.431370 5.2:+270518 None:intergenic
CTGATGACTTTCTACCCTGC+TGG 0.432241 5.2:-271246 MS.gene40543:CDS
AGCAGCTGGGGAAGACTGCT+GGG 0.434417 5.2:-269247 MS.gene40543:CDS
GTCTTACAATAGGAGCTTGT+CGG 0.435370 5.2:-264173 MS.gene40543:CDS
GAATCACACCAGCCTGGGGC+AGG 0.436578 5.2:-266557 MS.gene40543:CDS
TCTTACAATAGGAGCTTGTC+GGG 0.437681 5.2:-264172 MS.gene40543:CDS
CAACATTGGGTTCATACTAA+AGG 0.437824 5.2:+269576 None:intergenic
AATTGACAAGTGCTTAATAC+AGG 0.440713 5.2:-269143 MS.gene40543:CDS
GGGATGATAAGAAGAAAAGA+AGG 0.441760 5.2:+272296 None:intergenic
CCAGCTTTAACATGCGACTT+AGG 0.442294 5.2:+271541 None:intergenic
TTGGTTCCACTTGTTAGAGC+TGG 0.445940 5.2:-271337 MS.gene40543:CDS
TAGGTTATGGATAGCGATGA+TGG 0.448424 5.2:+272323 None:intergenic
GGCTGGCCTTGCCCAACAGC+AGG 0.455203 5.2:-270099 MS.gene40543:CDS
TAACTTCTAAGCTGCTCAAA+AGG 0.456111 5.2:+271101 None:intergenic
CTGTGCATTGATGTTGCTGC+TGG 0.456329 5.2:-271280 MS.gene40543:CDS
CTCAAAATCCTGAGAATTCG+TGG 0.456694 5.2:-269891 MS.gene40543:CDS
TAAACTCCTTGTAGGGATCA+TGG 0.457006 5.2:+271590 None:intergenic
CAAGCCTTTGCTGTTGCCGT+TGG 0.462562 5.2:-272117 MS.gene40543:CDS
AATGCTGAATCACACCAGCC+TGG 0.462675 5.2:-266563 MS.gene40543:CDS
ACAGTAGAAAAGTTGAAGTA+AGG 0.466027 5.2:+269505 None:intergenic
GCTTGAAAAGCCGCTCCAGC+AGG 0.466507 5.2:+271231 None:intergenic
TCAATAATAAACTCCTTGTA+GGG 0.467404 5.2:+271583 None:intergenic
TTGATGAAGTCCTCCAATAA+GGG 0.467454 5.2:-269940 MS.gene40543:CDS
TTGGAGTCTGATCAGCTTAG+TGG 0.470144 5.2:-270120 MS.gene40543:CDS
CAGTGGAAGCAATGGATTGA+AGG 0.473000 5.2:+272214 None:intergenic
ATGCTGAATCACACCAGCCT+GGG 0.475580 5.2:-266562 MS.gene40543:CDS
ATTATTCAAATGTCAACAAT+GGG 0.479031 5.2:-263589 MS.gene40543:CDS
ATGGCACATATAACCAATAA+GGG 0.481017 5.2:-266590 MS.gene40543:CDS
GGGATCTAATAATTTGGAAT+CGG 0.481778 5.2:-270232 MS.gene40543:CDS
ATTTCATGTGAATCACTGCC+TGG 0.482261 5.2:+269991 None:intergenic
AAAACATTTCTAGTCAACAT+TGG 0.484135 5.2:+269562 None:intergenic
ATCTACTTTGCAGCAGTATG+TGG 0.484679 5.2:-266245 MS.gene40543:CDS
GGCTTGATTAACAAGAGTAC+AGG 0.484679 5.2:+272134 None:intergenic
TCCTTGAGAAATCCAGGAAT+GGG 0.485830 5.2:+271364 None:intergenic
CAATCATGTGAACCGTATTG+TGG 0.486105 5.2:-271646 MS.gene40543:CDS
TAGCTTTAGCAATGACGTCT+TGG 0.487888 5.2:+271863 None:intergenic
TGATATCATTCCCTTATTGG+AGG 0.488899 5.2:+269930 None:intergenic
TCTTTCGATTTCTTAGGACT+GGG 0.491161 5.2:-267375 MS.gene40543:CDS
GTGCTTAATACAGGAAATCA+TGG 0.491229 5.2:-269134 MS.gene40543:CDS
AATGAGTCCTTGAGAAATCC+AGG 0.492551 5.2:+271358 None:intergenic
TGTTGTCCTGCTGTTGGGCA+AGG 0.494012 5.2:+270093 None:intergenic
GACTGGGTTACCATGTGGAT+TGG 0.495862 5.2:-267359 MS.gene40543:CDS
AACAGCAACGCGACATAACT+GGG 0.497467 5.2:+269723 None:intergenic
GAATTTGCTGACTTCTACAG+AGG 0.500296 5.2:-271835 MS.gene40543:CDS
CCTCTTTCAGCATCTGCTAT+CGG 0.500585 5.2:-267571 MS.gene40543:intron
CATGCAGGTGAAAGATATGA+TGG 0.501098 5.2:-265131 MS.gene40543:intron
GAGAGACTTCCAGAAATTCA+AGG 0.502668 5.2:-267685 MS.gene40543:CDS
TCCCATTCCTGGATTTCTCA+AGG 0.503476 5.2:-271365 MS.gene40543:CDS
CTTAGAAGTTAGATATCAGC+AGG 0.504295 5.2:-271089 MS.gene40543:intron
TAAGTAGATGCACAGCTATC+CGG 0.504455 5.2:+269409 None:intergenic
GGCTTCAAACCTTATGCTGA+AGG 0.505421 5.2:+266179 None:intergenic
GAGATGAAGGAAAGTGGTGT+TGG 0.507244 5.2:+272275 None:intergenic
AATCATGTGAACCGTATTGT+GGG 0.507781 5.2:-271645 MS.gene40543:CDS
CCTAAGTCGCATGTTAAAGC+TGG 0.512283 5.2:-271541 MS.gene40543:CDS
GTCTGATGAAACAAAGGCAG+TGG 0.518745 5.2:-264244 MS.gene40543:CDS
CACAATTATCACACGTGTCA+TGG 0.518950 5.2:-266219 MS.gene40543:CDS
TTGTCCATATAAACCTTACA+AGG 0.519142 5.2:+269475 None:intergenic
GGATAAATCAAGAGGTTTGG+AGG 0.520998 5.2:+272026 None:intergenic
GGAAGACGAGATGTCTCTGC+TGG 0.521174 5.2:-270196 MS.gene40543:CDS
TATCACACGTGTCATGGTGC+AGG 0.521265 5.2:-266213 MS.gene40543:CDS
TTGAATTTCTGGAAGTCTCT+CGG 0.521336 5.2:+267687 None:intergenic
TCCTGTCATCATGAAAATCC+AGG 0.523728 5.2:-270009 MS.gene40543:CDS
CGTCATTGCTAAAGCTACTT+CGG 0.531819 5.2:-271857 MS.gene40543:CDS
AGCAAGACACTAATAAGAAT+AGG 0.532277 5.2:-267627 MS.gene40543:CDS
AACAGGTTGGTCCTCACTCA+AGG 0.534971 5.2:+269678 None:intergenic
ATTGGCTGTGGTAATGAAGA+TGG 0.535024 5.2:-269358 MS.gene40543:CDS
TATTATTCAAATGTCAACAA+TGG 0.536777 5.2:-263590 MS.gene40543:CDS
AAGGAAAACGAGAGAAGCAG+TGG 0.536915 5.2:+272197 None:intergenic
GTATATGTAACCTACAAAAG+TGG 0.537483 5.2:-271903 MS.gene40543:CDS
TAGGTTACATATACTGGCAA+GGG 0.538830 5.2:+271912 None:intergenic
GTAGGTTACATATACTGGCA+AGG 0.540278 5.2:+271911 None:intergenic
TGATGCTTCACTGGATGTGG+TGG 0.541227 5.2:-269308 MS.gene40543:CDS
GATCTAATAAACCCACAATA+CGG 0.541700 5.2:+271634 None:intergenic
CCGATAGCAGATGCTGAAAG+AGG 0.543282 5.2:+267571 None:intergenic
GATATTTGAGAAAGAGTGAG+TGG 0.546501 5.2:+272236 None:intergenic
GTCCTTGAGAAATCCAGGAA+TGG 0.547420 5.2:+271363 None:intergenic
AGGTTGTTTGTGTACATGAA+AGG 0.550535 5.2:-267607 MS.gene40543:CDS
ATACTAAAGGGATTAATGCT+AGG 0.551971 5.2:+269589 None:intergenic
TCTTAATGTCAGTTGCGAGA+TGG 0.552190 5.2:-271391 MS.gene40543:intron
TACATCTGGTGGGAGCAGCT+GGG 0.552361 5.2:-269260 MS.gene40543:CDS
TTCTACAATCAATATATCCC+AGG 0.552523 5.2:-264130 MS.gene40543:intron
TAAGATGCCCTGCCCCAGGC+TGG 0.554845 5.2:+266549 None:intergenic
ATATTAAGATGCCCTGCCCC+AGG 0.556018 5.2:+266545 None:intergenic
GAGAGAAGCAGTGGAAGCAA+TGG 0.557632 5.2:+272206 None:intergenic
ATTTGAGAAAGAGTGAGTGG+TGG 0.559427 5.2:+272239 None:intergenic
AGTCTGATCAGCTTAGTGGC+TGG 0.559492 5.2:-270116 MS.gene40543:CDS
TTGGCTGTGGTAATGAAGAT+GGG 0.563722 5.2:-269357 MS.gene40543:CDS
AGATGAAGGAAAGTGGTGTT+GGG 0.565163 5.2:+272276 None:intergenic
CTGTGGTAATGAAGATGGGT+TGG 0.566586 5.2:-269353 MS.gene40543:CDS
ACATCTGGTGGGAGCAGCTG+GGG 0.568808 5.2:-269259 MS.gene40543:CDS
ACTAATCATCAATGTGCTTG+AGG 0.571703 5.2:-267946 MS.gene40543:CDS
AAGCACGATTCCAGAGATGA+AGG 0.574962 5.2:+272262 None:intergenic
TGTTGCCGTTGGAAATGTCT+TGG 0.575558 5.2:-272106 MS.gene40543:CDS
GTATCTTTGTTGAAAGTCAG+TGG 0.578801 5.2:+271187 None:intergenic
CCGGCGTATTATTAACAGGT+TGG 0.579081 5.2:+269665 None:intergenic
GCCGTTGGAAATGTCTTGGA+AGG 0.581630 5.2:-272102 MS.gene40543:CDS
TGTGTTTGTACAGATTGAAT+CGG 0.582351 5.2:+269444 None:intergenic
GATCAACGAAGTGACGGAGA+AGG 0.582631 5.2:+272178 None:intergenic
TGCTAGGTAGATTACACTGA+TGG 0.582733 5.2:+269605 None:intergenic
GTTCGTCTTCTGACATGAGC+TGG 0.582946 5.2:+270471 None:intergenic
TCTCTGCTGGAAGATATGTA+TGG 0.583056 5.2:-270183 MS.gene40543:CDS
TATATGTAACCTACAAAAGT+GGG 0.583341 5.2:-271902 MS.gene40543:CDS
CGTCCCATCTGCAAGTATCA+GGG 0.583813 5.2:-271506 MS.gene40543:intron
CTCCTATTGTAAGACAAGAC+CGG 0.583862 5.2:+264181 None:intergenic
GACTTTCTACCCTGCTGGAG+CGG 0.584490 5.2:-271241 MS.gene40543:CDS
GCACAATCTGCTGTTTGTTG+AGG 0.586706 5.2:+269538 None:intergenic
AAACACATATTCATTTCCAC+CGG 0.587649 5.2:-269428 MS.gene40543:CDS
GGATGATAAGAAGAAAAGAA+GGG 0.587713 5.2:+272297 None:intergenic
ATGTCAACAATGGGATGATG+AGG 0.587896 5.2:-263580 MS.gene40543:CDS
AACAAGAGTACAGGGAGGGA+GGG 0.589418 5.2:+272143 None:intergenic
GCTTCAAACCTTATGCTGAA+GGG 0.590254 5.2:+266180 None:intergenic
TTGCTGACTTCTACAGAGGA+GGG 0.593010 5.2:-271831 MS.gene40543:CDS
GAAGAAACTGGAGCATGCAT+AGG 0.594407 5.2:+270530 None:intergenic
AATCACACCAGCCTGGGGCA+GGG 0.596002 5.2:-266556 MS.gene40543:CDS
CATTGACAGTCATTCAGCAA+TGG 0.597321 5.2:-269830 MS.gene40543:CDS
TAACAAGAGTACAGGGAGGG+AGG 0.597701 5.2:+272142 None:intergenic
TTTGCTGACTTCTACAGAGG+AGG 0.598072 5.2:-271832 MS.gene40543:CDS
TGCTGACCAGCTCTAACAAG+TGG 0.601124 5.2:+271331 None:intergenic
ATTTCCAACGGCAACAGCAA+AGG 0.602720 5.2:+272113 None:intergenic
GGAATCGGATGTCTCAAGTA+CGG 0.604609 5.2:-270217 MS.gene40543:CDS
TTGTCAATTATAATATCAAG+TGG 0.604760 5.2:+269157 None:intergenic
AGCCCAAAATTCACATGCCT+AGG 0.605045 5.2:+263647 None:intergenic
ATTCCAGAGATGAAGGAAAG+TGG 0.605425 5.2:+272269 None:intergenic
CTTAGGACTGGGTTACCATG+TGG 0.605914 5.2:-267364 MS.gene40543:CDS
GTGGGGACTCTAGACAAACT+AGG 0.606813 5.2:-270494 MS.gene40543:CDS
TGAAATGATCAACGAAGTGA+CGG 0.608813 5.2:+272172 None:intergenic
GTTACCCTGATACTTGCAGA+TGG 0.613096 5.2:+271502 None:intergenic
TTAATATATTGATGAAGATG+AGG 0.613751 5.2:-266529 MS.gene40543:intron
TCTGGATTGACAGTTCAAGT+AGG 0.618169 5.2:+267662 None:intergenic
TGAAATCCATGATCCCTACA+AGG 0.620840 5.2:-271596 MS.gene40543:CDS
TCTGCTATGTCAAGGAACCT+AGG 0.621862 5.2:-263664 MS.gene40543:CDS
ACCTTCCAAGACATTTCCAA+CGG 0.622034 5.2:+272101 None:intergenic
TTTGTGTACATGAAAGGACA+TGG 0.624900 5.2:-267601 MS.gene40543:CDS
CTTGAAAAGCCGCTCCAGCA+GGG 0.624998 5.2:+271232 None:intergenic
TCTGATGAAACAAAGGCAGT+GGG 0.626342 5.2:-264243 MS.gene40543:CDS
TGTTAGTGATTCTTTGAGTG+TGG 0.628800 5.2:-269704 MS.gene40543:CDS
GATTATGGAGAAATTGGCTG+TGG 0.635510 5.2:-269370 MS.gene40543:CDS
TACAGAGTGGCCAATCCACA+TGG 0.637678 5.2:+267349 None:intergenic
GCTTGATTAACAAGAGTACA+GGG 0.645927 5.2:+272135 None:intergenic
CTAGACATGGATAAATCAAG+AGG 0.650742 5.2:+272018 None:intergenic
TTACCCTGATACTTGCAGAT+GGG 0.651039 5.2:+271503 None:intergenic
AATCATCAATGTGCTTGAGG+AGG 0.655724 5.2:-267943 MS.gene40543:CDS
ATTGCACGTCTGCTATGTCA+AGG 0.656079 5.2:-263672 MS.gene40543:CDS
GTCTGAGAATGAGAGAAGCG+TGG 0.661132 5.2:+272055 None:intergenic
GTAGATGCACAGCTATCCGG+TGG 0.668645 5.2:+269412 None:intergenic
AGTGTTGAAACAGCTAGACA+TGG 0.670789 5.2:+272005 None:intergenic
TGCTGAATCACACCAGCCTG+GGG 0.674390 5.2:-266561 MS.gene40543:CDS
TTCATCCTTAAACAGCTCTG+AGG 0.675574 5.2:-270148 MS.gene40543:CDS
GATTAACAAGAGTACAGGGA+GGG 0.678234 5.2:+272139 None:intergenic
TTGATTGTGATGCTACTCAA+AGG 0.694455 5.2:-269207 MS.gene40543:CDS
CCAACCTGTTAATAATACGC+CGG 0.697464 5.2:-269665 MS.gene40543:CDS
GAGTGTGGAAACCTTGAGTG+AGG 0.699788 5.2:-269689 MS.gene40543:CDS
CTTACAATAGGAGCTTGTCG+GGG 0.705265 5.2:-264171 MS.gene40543:CDS
TGTCCATATAAACCTTACAA+GGG 0.706395 5.2:+269476 None:intergenic
GGTGTCAAAATAATACAGAG+TGG 0.715615 5.2:+267336 None:intergenic
AAATTCAGTATTGAACAGAG+AGG 0.745412 5.2:-269859 MS.gene40543:CDS
TGATTAACAAGAGTACAGGG+AGG 0.760574 5.2:+272138 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 263565 272373 263565 ID=MS.gene40543
chr5.2 mRNA 263565 272373 263565 ID=MS.gene40543.t1;Parent=MS.gene40543
chr5.2 exon 271798 272373 271798 ID=MS.gene40543.t1.exon1;Parent=MS.gene40543.t1
chr5.2 CDS 271798 272373 271798 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 271507 271713 271507 ID=MS.gene40543.t1.exon2;Parent=MS.gene40543.t1
chr5.2 CDS 271507 271713 271507 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 271090 271401 271090 ID=MS.gene40543.t1.exon3;Parent=MS.gene40543.t1
chr5.2 CDS 271090 271401 271090 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 270431 270555 270431 ID=MS.gene40543.t1.exon4;Parent=MS.gene40543.t1
chr5.2 CDS 270431 270555 270431 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 269130 270254 269130 ID=MS.gene40543.t1.exon5;Parent=MS.gene40543.t1
chr5.2 CDS 269130 270254 269130 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 267845 267986 267845 ID=MS.gene40543.t1.exon6;Parent=MS.gene40543.t1
chr5.2 CDS 267845 267986 267845 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 267572 267731 267572 ID=MS.gene40543.t1.exon7;Parent=MS.gene40543.t1
chr5.2 CDS 267572 267731 267572 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 267242 267405 267242 ID=MS.gene40543.t1.exon8;Parent=MS.gene40543.t1
chr5.2 CDS 267242 267405 267242 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 266530 266615 266530 ID=MS.gene40543.t1.exon9;Parent=MS.gene40543.t1
chr5.2 CDS 266530 266615 266530 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 266189 266288 266189 ID=MS.gene40543.t1.exon10;Parent=MS.gene40543.t1
chr5.2 CDS 266189 266288 266189 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 265085 265146 265085 ID=MS.gene40543.t1.exon11;Parent=MS.gene40543.t1
chr5.2 CDS 265085 265146 265085 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 264131 264275 264131 ID=MS.gene40543.t1.exon12;Parent=MS.gene40543.t1
chr5.2 CDS 264131 264275 264131 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
chr5.2 exon 263565 263735 263565 ID=MS.gene40543.t1.exon13;Parent=MS.gene40543.t1
chr5.2 CDS 263565 263735 263565 ID=cds.MS.gene40543.t1;Parent=MS.gene40543.t1
Gene Sequence

>MS.gene40543

ATGAATGCAATGTTAGGTTCCAAATCATCCATCATCGCTATCCATAACCTATCTCCCTTCTTTTCTTCTTATCATCCCAACACCACTTTCCTTCATCTCTGGAATCGTGCTTCCACCACTCACTCTTTCTCAAATATCCTTCAATCCATTGCTTCCACTGCTTCTCTCGTTTTCCTTCTCCGTCACTTCGTTGATCATTTCACTGTTTCCCTCCCTCCCTGTACTCTTGTTAATCAAGCCTTTGCTGTTGCCGTTGGAAATGTCTTGGAAGGTTACATTTCTGCTCTCCACACTATCCACGCTTCTCTCATTCTCAGACGTGCTTCCTCCAAACCTCTTGATTTATCCATGTCTAGCTGTTTCAACACTGTTGCTCATTCTGAAATTACGTTGCTTGAGTTATATCTGCACACAAAACAACTCAGAATTCAGATTCAAGCCCTTGCCAGTATATGTAACCTACAAAAGTGGGCTCATTATACTGATTTCCAAGACGTCATTGCTAAAGCTACTTCGGAATTTGCTGACTTCTACAGAGGAGGGACCTTATTAACATTTTTGTATCATCAATTACAGGTGTGTATATATAAGTATAGATAATGACCTGACTAAAATGCAATTTCATGATTCATAAATGAACTTATGCATGTTTCTTTTTAGGTTGCTGATTCTGCTCACTGTGCTCTTCTCAAGTTTCTTTTTCTTCAATCATGTGAACCGTATTGTGGGTTTATTAGATCTTGGATTTTCAAAGCTGAAATCCATGATCCCTACAAGGAGTTTATTATTGAAAATATAGAATGTTTATCTCCTAAGTCGCATGTTAAAGCTGGTAATTCTGTTGACGTCCCATCTGCAAGTATCAGGGTAACTCTTTTCTGTGCTGACCTGCAGCCTGTTTTTTCATGTGTATCAATAATGGACTATTGCCTTGTTATATTGATTCTCATAGTGAGAACTTCTTAATGTCAGTTGCGAGATGGAGTTCCCATTCCTGGATTTCTCAAGGACTCATTGGTTCCACTTGTTAGAGCTGGTCAGCAGCTTCAAGTACTATTGAAATTACTTGAGCTGTGCATTGATGTTGCTGCTGGACAACATAGTTCTGATGACTTTCTACCCTGCTGGAGCGGCTTTTCAAGCAACAGTCTGTCTTATTTCTCTCCACTGACTTTCAACAAAGATACTATAGATAATATGGTGCTTGCAAGAGAAAGCTACTATAGAAGGATGAATGAAAAAATTGAAAGCCTTTTGAGCAGCTTAGAAGTTAGATATCAGCAGGTAATCGTACTGCATAGTTTTGGTTTCTTGTACAGTGAACTTTTTATCTCTATTTACTTTCAGTTATTTTGTGGTACCAATACATAATTTACTTTCAAGAGAATAGAAAGGGATTGCAAGGCCAAAAATTCAAGATATTATCTTACAGGTACCAATGGATGAAGAAATGCAGAAAAGGCGTCAGATTTTTATTTTATGAAGAAAAAAAACTATAAGCAAATTCACTTAAAACGGATGTAGGTCATACATAAGGAAGAAAACAAATTATTGTGATACTTTCTAACTAAATCCATCCCATTATTGATTTAGAATTATGTCTGTACCAAGATCTTTTCCTTCTTGTTTATGTCAGATGATATTAAAAATATCCTATAATATTTGGATCTTATCTTCAGAGTATCTTAGATTATGTCTTATGATTTGTTTTCATACTTCTTAGATGGAGAGCAGAAAAAACTGCTTTTCCCACTACGAGTAGGTACTTATTTTCTCTCTGTTTTTCGTTCTCATGCTTGAAGAATTTCCTTTTTGCAGGTACCTATGCATGCTCCAGTTTCTTCTTTTGATAATGATGTGGGGACTCTAGACAAACTAGGCCAGCTCATGTCAGAAGACGAACCTATTATCTCTCCAACAGAAGATAAAAGTTCTTCAAATATGTAATCTCTTACTTTCATTCCCCTCCTCCCACCCCCAGGAAAAATGTGTGTGCATGAAGATGCATGCACTCAATAACATGTTTATCTCATTCTCTGCTTGACATATTAAATATTTGTCCTTACGACTTTTATTTCCCTTTGTTATTTCGTCATTGATGTTTTGTAGAAATTCTCAGGGGATCTAATAATTTGGAATCGGATGTCTCAAGTACGGAAGACGAGATGTCTCTGCTGGAAGATATGTATGGTCAATCTGAAAGTTCATCCTTAAACAGCTCTGAGGAGCAATTGGAGTCTGATCAGCTTAGTGGCTGGCCTTGCCCAACAGCAGGACAACAAAATCATCTATCTGCTTTAAGCTTCTTGAAGTTCACTACCTTAAATAGTTCAATACAAAATTCCTGTCATCATGAAAATCCAGGCAGTGATTCACATGAAATATGTGATAAAATGGACGCTGTCGATCATTTGATGAAGTCCTCCAATAAGGGAATGATATCAAGTCACATGTTTGACCCTCAAAATCCTGAGAATTCGTGGTACTCAAGTAAATTCAGTATTGAACAGAGAGGTAGTTGCATTGACAGTCATTCAGCAATGGACGATTTACTGAAAAAATCATTTGATGCTGATGGAACTGTTGAGCAAAAGATGACAGAAAAGCACCTGCAATCCATGAAATATTCCCAGTTATGTCGCGTTGCTGTTAGTGATTCTTTGAGTGTGGAAACCTTGAGTGAGGACCAACCTGTTAATAATACGCCGGCTTCATTTTTGTGTGATTTTCAACCACTAAAAGTTGGCCATCAGTGTAATCTACCTAGCATTAATCCCTTTAGTATGAACCCAATGTTGACTAGAAATGTTTTGCCTCAACAAACAGCAGATTGTGCACAGACATTTCCTTACTTCAACTTTTCTACTGTAGAGGACCCTTGTAAGGTTTATATGGACAAGTTACTCACCGATTCAATCTGTACAAACACATATTCATTTCCACCGGATAGCTGTGCATCTACTTATGGTAATCAGAATAATGATTATGGAGAAATTGGCTGTGGTAATGAAGATGGGTTGGTTGATGAACCCAAATATGGTTTTGATGCTTCACTGGATGTGGTGGACCATAAACAATATGTTTTAACAGATACATCTGGTGGGAGCAGCTGGGGAAGACTGCTGGGCAGTTTTCGGAAAACTGTTGATTGTGATGCTACTCAAAGGCAGACTTCGTTGTCAACATTTGAGATGCCACTTGATATTATAATTGACAAGTGCTTAATACAGGAAATCATGGTTCAGTATCCTTATTTTTATATTTATTCTGGTAACCTAATTGAATGGTTTAGGTATGCTGTTTTATATTTCATTGCTTTATAGCTTCTATTATTTCTTAGCATGATACTCGATAGGTATACTATACAAGAACTTGGGGAACCCTCCCTTTGATGTGGGAGTTGGGATTTAGGCACTCCATAATGCCCAAGTCTCTATCACAATATGCCCTTGAGAATGATAGGCGATGTCACAACAACTACACTTTACAATGCTTTACTCGGCCTTTTCAGTCAGTTCTTTCTTAGGTAGTTTTTCGAGCTACCGACAAATCTGATACTAATTGATATGTATCCTAGCACTTGGGGAACCCTTCCTTAAAAGCTAGCTATCAAGCGGAAGAACCAAACCACTTAAATACTCCACAAAGCATCCCCCGCTACTCGATGTGGGACTTAGGCACCCAGTAATATCTGGGTCCTTTCAATACTGTTCATGCATCGGTAATGTTGTCAGTTGTATTATCTTCTATAAATTAGTTAATGCAGCCTCAGTTAGCTTCTTTACTGATGGGTTAGAAAGTTATACAATCAACAAGCAAAAAATCACAAAGGAAATGCAAATATTACTAATTCCTAGTACTATATTTTATATTTTGAAATAAAAGAACGATTTAGTGGTGCTTCAATATATTTTCAAAAAAAAAAAAAAGCATACCATACCCCATCAGGTAGGACAAGCTACATGAATTAATTGATGCCATTTAGAATTCATGCTCCATAGTAAATTTGTGCTATGAGTATTTGTAAAGAGTCCCACATCGGACAAAAGATGGTCTAAGCATGTGTTTATAAGTGGGGCCAGTCCTCACCTCACAAGCCGGTTTTGTGAGGTTGTATTAGGCCCAACCTTTCTAAGAGTATTATTGTACCAAACAAAGTGCAAAATATACAGTAAGCCTGTATAACATGTAATATGTTGAGGCGGACAATGGTAATTTATTGGTCATGTTTAGAATTTATGTGTGAAAGTTGACAGATGAAACAGTGTAGAATAAGCATTATACAATACGTTGAATTTTATCAATATTCTCAGATTCAAATGGGTTGGTATGATATTTCTTTTGGAAAGTATTATGTAATAGCTTTGCGTGTCCTTAAAGATGCTAGATACAATTATGTCAGCAAACTAATCATCAATGTGCTTGAGGAGGCATTTAAGTTGCAAGAGCATCTTTTAGCACTGCGGCGGTATCATTTTATGGAATTAGCAGATTGGGCAGATTTGTTCATCTTGTCTCTTTGGCGACATGTATTCCTAAATTCTGATTGACAATGACTTCCTTAATATTTCATAGCTTTGATTTCTGACTATAGTCTACAATCTGAACGTTTATTCATTCTTTGTTTTACTTAAAATCACAGAAGTGGTCTGTTACAGAAGCTACCGAGAGACTTCCAGAAATTCAAGGCCTACTTGAACTGTCAATCCAGAAGTCTTCTTGTGAGCAAGACACTAATAAGAATAGGTTGTTTGTGTACATGAAAGGACATGGAAAATTGCCTCTTTCAGCATCTGCTATCGGTATATCTATACTTCTACTACTAGAGATCCGGTATTTTGTTTTTATTATTGTCTTATTTGGTTTCATTAATATTTTCATTTTCATTTCAAGTTTGACAAGCTCACCCCACCCCTCCGTGGAAGGTTAAATTATGCTTCTGATAAGGAAATTCGTAATTAATGGCAGGGGTTCGTTCTTTCGATTTCTTAGGACTGGGTTACCATGTGGATTGGCCACTCTGTATTATTTTGACACCTGCTGCGTTAAAAATATATGCTGATATATTCAGCTTTTTAATACAAGTGAAGCTTGCCTTATTTTCATTGACTGATGTGTGGTGCTCATTAAAGGTTTGGTTTCTTGCAGCCGTTCCCTTTTTCTTTTCCTGTTGTTGTTATTGCATAACTGCATTTATTTTTATCCTCAGTAAGATTTTGATTTAGCAATTTCGTGGCAGGAAATTTCCAATTTAAAACAATTGAACACTTAGGTTAAATATGTCCAAAGAGTTATCGGCGAGTAACTATTTATTGCCTTAATGTTTTCTAAACTAAATTTTTCCACTAATTTTGAGTGAGGACTGTCAAAGTCTTATTGGATTACTTTGGTCTATCTTTAGTTGATGTGGTTTTTCAAATTTTCAACACCCCCTCATGCATAGGATTAGATATTTCACATTTGGAGTGAAGTTTTGCAATTTTGGACATGCCACATGTTGGATCGACTCTAATAGACTAATACCATCTCAAAGTTTGGACATGCCTTGAATGTTTCTCTATACTTCATTACGAATAACTTTGGAACAATGTCTGAGAGCCTGCAATTTCATAAGTCTGCAGCATCATTCTGAAGTGTATAATCTGATATCCCGAATTGTTTCTATTGCTATGAGTTACATTTATATTCTGATTTTTCAAGGGAATAAGGGATTTTGATTTTATTGCAGTGCTTATTTTCTTTCAATTTTGGTGAACAGGATATGGCACATATAACCAATAAGGGTCTAAATGCTGAATCACACCAGCCTGGGGCAGGGCATCTTAATATATTGATGAAGATGAGGTATATGTACTGGATTTTTTTGGGAAGTCTGGGACCATGAACTATTTTTTATATGATAGGCCTTCTTCTGTGGTCATATTATCGTCTTTAATGTATGCCTTGGAGGTTTGGACCATGTGGTCTAGGTGGGGACACTGTCTTGATGAAGTCCCGTGAAATCTTGAATATTGAATATTGAATATGAGCTTCTTTATATGTATATTAGCACCTCTAAGCATCCTTTCTCCACTTTCATTGACAGGCACCAGATCAGCCATTTTGTATCTACTTTGCAGCAGTATGTGGAGTCACAATTATCACACGTGTCATGGTGCAGGTTTCTTCATTCCCTTCAGCATAAGGTTTGAAGCCAATGCATCTATTTATTTAGTTTCATTTCTTTGTATCCTTTTCTTCAATGTATAAATTGATGCGTTTATGGAGCTTTATAAGTATTTATTTTCAGCGCAAAATCACCTTTTCTGAACTGCTTATGTATTCTGATCTGTGTCTTAATAACTGTGGTCGCTTAGTCGAAATGAAGGTCTGGCTTTTACTTTCTATCTTTTGGTTAAGCTCACTGCATACAACTGCATGGGCTGTGCCCTTCTTAGGAGACTGTATTTCTGTTTTTATTGTGGGAGAACTTGATATAAGACAGGAAATAAGAGAAAAAAGATAAAGACATCCTGAACATAATTCTTTAGGTTGCATTTGACTCACATCTCATTTCTAATCTAGTTCTACAAGCACTATTAGGAAACCAGTTTTAATCCTGTAATTCCAGGCTTTTAGCTTCCTCTGCTTTGGAACAGTTTCTGCTGGTTAAAGTTAAATGTTTTAGTACCAGGTTTTGGGCTGTGTGCAATTTATATTATTCTTTGGTTTGAGAAGAATGCTTGCTTTCTTTAAGGAGTATCTCATTACCCTTTCTTTAAAGAGGGCCAATGGGGTTACGGGCAAAATATCTGTTTTTAGGGTTATCTTCTTTGACGACGCCAATCTGCCACATGTTTTTGAGCTGTACCTGCCTGTTTATTTCTTTATTTTATTAATTATTAATGAATGCTACCTCTGGTCCCATATATGAGAAGAAAAATTCAGGACTAATTTAACGCTACAGTTTCGAAAGTTCATTCCAAATATTACCTCGTCATTAAATGTTCCCCCCACCCACTTACATGGAGAAAGATGTATTTCTAGTAAACATGTGAAGGATTTTAAAGTAGTTTCAGCAATTAATACATCTTAAAATTTGTGGCTTTTTCTTAGAATTGGGAACGAAAAAAGCACCAAACATTTTCGTAAAAATTAAATACTCTACACGGCTAGATATGATGATGTGTATGACTTCAAAATTCAATCACTATATCAATGTTTTGGATATTCCCGTCATTCATGCAGGTGAAAGATATGATGGATCTCGAGTCAGTGCACACAGAATATCTTGCTGATTCGTTAAGCATGTAAGTTTACATTTAATTGCTACTTATTATTTATACAGACAGTTACTAGAAAGTCAGTTTTGAGAAAGTATTTTGGTAATTATGATTACTTAGCTAACAAATTGAATCATGAATTTGTCTAATCTGGTTTTCCTAGTCAAGATCTTCACCATTATCTGCCTGCATGCCACCCTCTAGGCAAGCTCTTGCTAGGCTTGGCAATTGTGGCTTGGGGGGGGAATAGTTCTTCCGTCCACATCCACAATTTCATTGCATTATCTTCAACCCAGCTCTATTTCCTGTGTGGTTACTCCTTCACAAATTGACTGACATGGATGATTTCTAAATTCTTTTTTCAACATCTGACACCCATCACTGCCCCAACATAAGCCCTAGTCAAAGTGGATTTTTCCCATTAATGTCGGGGTGGGCTTTGGTGGGTATCCACGGGGTTTGGTCCTCCGTTGTAATACTGATTACTAGTTAACTAGGTATTTGCTTACTGATTAATTTGCAATCGATCAGTATTGTAATATTAATAACTGGTTATCTGCATACTGATCAATAGTGTAAAGCTTATGTGCAAGTTTGATGTGTAAATAGGGTAACTATTTAGTGCAAGTTTTTGAACTTGTACCCACCCACAAAAAAACCTTTGGATTTGGAAGTTTTCTTCCACAACTCCTAAAGAAATTTACAGCAAGAGGAAAAAAGAACATTATAGTGTCACGTGTCATAATTTTAGTTGGAAGACATACGCACTATAGGACTAAAAATATTTTCCATAAGCTCAGAGGGTAAATAAGAGGTTTATGCATGTATTATTGCAGATGTTTTCTGTCTGATGAAACAAAGGCAGTGGGCAGCATTATTGAGAGCATTTTGCAATGTGCACTTGATTTCCGGTCTTGTCTTACAATAGGAGCTTGTCGGGGAGACTTTTTAGGAGAACTTTCTACAATCAATATATCCCAGGTAAACCATGCACCATATGCATCACATATCTGATACATTTATACTGTTTTCCAGCATATTGGCGTCTCAATTATAGACTTCGCGAGTCATTACTCTTAACTGTCATTGCTGTGTATGGCAATATGTATACGTCCCTTTATTCTACAAAACTTCTCAATGTTCACACTTATTATTTCTAGGGTCTGATTCCCTCAAAACAACCTTATCTTTGCCAAAAGGCCTTTTTGAAAAACATACGCATTAGATAAGAACATAACAGAAAATGGAACATCCTATCTTGAACATGTTAGTTATGTCATCAAATATTTTGATCCTATCCGAGTAAACAATACACTGACCAGCTGATGGGAACCAAATATTGGATCCTAACACCAGCTGTTTCTAATTATTTCCAGGTGCTTTCCATAAAGCAAAAGTTTGAAAGAAGTCTAAATGAATTGCACGTCTGCTATGTCAAGGAACCTAGGCATGTGAATTTTGGGCTTTCTCGTTTCTGGGAATATCTCAACTATAACGAATATTATTCAAATGTCAACAATGGGATGATGAGGTACTGTGCTGTCTGA
Protein sequence

>MS.gene40543.t1

MNAMLGSKSSIIAIHNLSPFFSSYHPNTTFLHLWNRASTTHSFSNILQSIASTASLVFLLRHFVDHFTVSLPPCTLVNQAFAVAVGNVLEGYISALHTIHASLILRRASSKPLDLSMSSCFNTVAHSEITLLELYLHTKQLRIQIQALASICNLQKWAHYTDFQDVIAKATSEFADFYRGGTLLTFLYHQLQVADSAHCALLKFLFLQSCEPYCGFIRSWIFKAEIHDPYKEFIIENIECLSPKSHVKAGNSVDVPSASIRLRDGVPIPGFLKDSLVPLVRAGQQLQVLLKLLELCIDVAAGQHSSDDFLPCWSGFSSNSLSYFSPLTFNKDTIDNMVLARESYYRRMNEKIESLLSSLEVRYQQVPMHAPVSSFDNDVGTLDKLGQLMSEDEPIISPTEDKSSSNMGSNNLESDVSSTEDEMSLLEDMYGQSESSSLNSSEEQLESDQLSGWPCPTAGQQNHLSALSFLKFTTLNSSIQNSCHHENPGSDSHEICDKMDAVDHLMKSSNKGMISSHMFDPQNPENSWYSSKFSIEQRGSCIDSHSAMDDLLKKSFDADGTVEQKMTEKHLQSMKYSQLCRVAVSDSLSVETLSEDQPVNNTPASFLCDFQPLKVGHQCNLPSINPFSMNPMLTRNVLPQQTADCAQTFPYFNFSTVEDPCKVYMDKLLTDSICTNTYSFPPDSCASTYGNQNNDYGEIGCGNEDGLVDEPKYGFDASLDVVDHKQYVLTDTSGGSSWGRLLGSFRKTVDCDATQRQTSLSTFEMPLDIIIDKCLIQEIMVQYNYVSKLIINVLEEAFKLQEHLLALRRYHFMELADWADLFILSLWRHKWSVTEATERLPEIQGLLELSIQKSSCEQDTNKNRLFVYMKGHGKLPLSASAIGVRSFDFLGLGYHVDWPLCIILTPAALKIYADIFSFLIQVKLALFSLTDVWCSLKDMAHITNKGLNAESHQPGAGHLNILMKMRHQISHFVSTLQQYVESQLSHVSWCRFLHSLQHKVKDMMDLESVHTEYLADSLSICFLSDETKAVGSIIESILQCALDFRSCLTIGACRGDFLGELSTINISQVLSIKQKFERSLNELHVCYVKEPRHVNFGLSRFWEYLNYNEYYSNVNNGMMRYCAV