Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40775.t1 | XP_024638755.1 | 98.8 | 487 | 6 | 0 | 1 | 487 | 1 | 487 | 4.00E-285 | 989.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40775.t1 | F4HZG9 | 68.4 | 472 | 143 | 3 | 1 | 472 | 1 | 466 | 6.9e-193 | 674.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40775.t1 | A0A072VM73 | 98.2 | 487 | 6 | 1 | 1 | 487 | 1 | 484 | 1.1e-281 | 978.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene40775 | MS.gene003186 | PPI |
| MS.gene40775 | MS.gene02160 | PPI |
| MS.gene006974 | MS.gene40775 | PPI |
| MS.gene40775 | MS.gene02843 | PPI |
| MS.gene40775 | MS.gene048313 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40775.t1 | MTR_1g084190 | 98.152 | 487 | 6 | 1 | 1 | 487 | 1 | 484 | 0.0 | 994 |
| MS.gene40775.t1 | MTR_8g101730 | 26.582 | 474 | 285 | 16 | 54 | 467 | 8 | 478 | 2.36e-37 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene40775.t1 | AT1G09300 | 68.432 | 472 | 143 | 3 | 1 | 472 | 1 | 466 | 0.0 | 685 |
| MS.gene40775.t1 | AT1G09300 | 70.794 | 428 | 123 | 1 | 45 | 472 | 10 | 435 | 0.0 | 652 |
| MS.gene40775.t1 | AT4G29490 | 25.176 | 425 | 259 | 14 | 96 | 467 | 59 | 477 | 1.99e-37 | 144 |
Find 130 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTAGCTCTCAAGTTATTT+TGG | 0.237616 | 1.2:+59301217 | None:intergenic |
| CTCACATAAGGTTTGGCTTC+TGG | 0.259772 | 1.2:+59309223 | None:intergenic |
| TGAATTTGCTTCACTTCTTT+CGG | 0.281191 | 1.2:+59301282 | None:intergenic |
| ACCAATGTCATGCCCTAAAA+TGG | 0.283309 | 1.2:+59309261 | None:intergenic |
| TAGAAGTTGGGTTCAGCTTA+TGG | 0.298215 | 1.2:+59305153 | None:intergenic |
| GAGGAACCCCTTATCATATC+TGG | 0.302925 | 1.2:+59308086 | None:intergenic |
| ACATCTGTCATCATCTTTAC+TGG | 0.311255 | 1.2:+59309355 | None:intergenic |
| AATTAGCATTTGCAGGTTCT+AGG | 0.321480 | 1.2:-59310457 | MS.gene40775:intron |
| TTTGAATAATTAGCATTTGC+AGG | 0.333705 | 1.2:-59310464 | MS.gene40775:intron |
| GCCGATTACTCATATATTAC+AGG | 0.336669 | 1.2:-59309307 | MS.gene40775:CDS |
| ACAACTTCAGCCATGGAATA+GGG | 0.337029 | 1.2:-59301248 | MS.gene40775:CDS |
| ACCTCGTTCAACTTTCTCAT+TGG | 0.343552 | 1.2:+59308333 | None:intergenic |
| GCATCTTGTCGAAATGTATA+AGG | 0.350875 | 1.2:+59309328 | None:intergenic |
| ATTGATCCAGGTCACTATCT+AGG | 0.354401 | 1.2:-59305006 | MS.gene40775:intron |
| GACTGCTACATCAGCATATA+CGG | 0.360739 | 1.2:-59308042 | MS.gene40775:CDS |
| AATTTGTCTAGGATGTGATT+TGG | 0.363924 | 1.2:-59308416 | MS.gene40775:intron |
| CTTTCGGAATTGATGCTGTT+AGG | 0.367090 | 1.2:+59301298 | None:intergenic |
| GAAGTGTTATACATTATTCT+AGG | 0.368162 | 1.2:-59307387 | MS.gene40775:CDS |
| ATCTGCATCAATTGCTTGTC+AGG | 0.376334 | 1.2:-59307907 | MS.gene40775:intron |
| ATGATAGCCAAACTCTTCTC+TGG | 0.383373 | 1.2:+59309388 | None:intergenic |
| GAGATTCTCATTACAGAAAC+AGG | 0.389989 | 1.2:-59302411 | MS.gene40775:CDS |
| CTGTTTGTGTGAAAATGTTT+TGG | 0.397576 | 1.2:+59310583 | None:intergenic |
| TTCAATCCCGTGGTTGGTGG+TGG | 0.398757 | 1.2:-59307418 | MS.gene40775:CDS |
| CATGTTTGGCAGATTAAAGA+TGG | 0.406377 | 1.2:-59307293 | MS.gene40775:intron |
| AAGCTGAACCCAACTTCTAT+AGG | 0.407794 | 1.2:-59305149 | MS.gene40775:intron |
| ATGAGAGGTGCGCAGCGAAT+GGG | 0.410718 | 1.2:-59307649 | MS.gene40775:CDS |
| AACAACTTCAGCCATGGAAT+AGG | 0.413497 | 1.2:-59301249 | MS.gene40775:CDS |
| GTGGTTGGTGGTGGGCCTAA+TGG | 0.419819 | 1.2:-59307409 | MS.gene40775:CDS |
| ACAGTAAGCTAGTTTCTTGA+AGG | 0.421447 | 1.2:+59308010 | None:intergenic |
| GCCTGTAATATATGAGTAAT+CGG | 0.422629 | 1.2:+59309306 | None:intergenic |
| GCAGCTAGCATACCTTCAAA+AGG | 0.428825 | 1.2:+59307694 | None:intergenic |
| GATCCTTCCAGATATGATAA+GGG | 0.429047 | 1.2:-59308093 | MS.gene40775:CDS |
| AGTACCCGTGGGTGAGATGC+AGG | 0.431157 | 1.2:+59310382 | None:intergenic |
| TCTGGACAATTGAAAGAAGA+TGG | 0.434360 | 1.2:+59303885 | None:intergenic |
| GCATTGTAGCTTCTAAAAGA+TGG | 0.435409 | 1.2:-59309478 | MS.gene40775:intron |
| TATCAGACTCACATAAGGTT+TGG | 0.437944 | 1.2:+59309216 | None:intergenic |
| AGATCCTTCCAGATATGATA+AGG | 0.439022 | 1.2:-59308094 | MS.gene40775:CDS |
| GCTTCTCCCAGAGAAGAGTT+TGG | 0.441988 | 1.2:-59309395 | MS.gene40775:CDS |
| CATTACAGAAACAGGTTATG+AGG | 0.448108 | 1.2:-59302403 | MS.gene40775:intron |
| ATCAACGGAAACCTTGTGAT+TGG | 0.449498 | 1.2:+59310420 | None:intergenic |
| CATCTGTCATCATCTTTACT+GGG | 0.449826 | 1.2:+59309356 | None:intergenic |
| AACTTTAGTAAGTACCTCTC+TGG | 0.452530 | 1.2:+59303867 | None:intergenic |
| GGCTATCAACCCCTATTCCA+TGG | 0.458885 | 1.2:+59301238 | None:intergenic |
| CATTGTAGCTTCTAAAAGAT+GGG | 0.460019 | 1.2:-59309477 | MS.gene40775:intron |
| CCTAAAATGGCCACACCACC+AGG | 0.460454 | 1.2:+59309274 | None:intergenic |
| TGGTAGCTTCTCTTCTGCCC+AGG | 0.468113 | 1.2:-59307195 | MS.gene40775:intron |
| GGTACCAAGGCATTGGGATA+AGG | 0.470535 | 1.2:-59302443 | MS.gene40775:CDS |
| CCGAATGGTTGTGTATTTGT+CGG | 0.470570 | 1.2:+59306196 | None:intergenic |
| TGCAGCATTGAATACATTCA+AGG | 0.471710 | 1.2:-59308370 | MS.gene40775:CDS |
| CACAAGGTTTCCGTTGATGT+TGG | 0.472218 | 1.2:-59310415 | MS.gene40775:CDS |
| TAATGCAGGTACCAAGGCAT+TGG | 0.480015 | 1.2:-59302450 | MS.gene40775:intron |
| TTCATCAGCTTGAGCTCTGA+AGG | 0.481437 | 1.2:+59307933 | None:intergenic |
| TGTGTGGAACTTTGTAAGCC+TGG | 0.488945 | 1.2:-59306228 | MS.gene40775:CDS |
| TTTGACTGTCCTCTGAAGCC+AGG | 0.489111 | 1.2:-59304952 | MS.gene40775:CDS |
| ACCAGGCTACCTATAGAAGT+TGG | 0.492131 | 1.2:+59305140 | None:intergenic |
| GTCTAGGATGTGATTTGGCA+AGG | 0.493497 | 1.2:-59308411 | MS.gene40775:intron |
| AACATTGTGATACAATTTCG+AGG | 0.498748 | 1.2:+59308067 | None:intergenic |
| AATGAGAGGTGCGCAGCGAA+TGG | 0.501064 | 1.2:-59307650 | MS.gene40775:CDS |
| TGGCCACACCACCAGGTTGT+TGG | 0.502948 | 1.2:+59309281 | None:intergenic |
| TAATGTATAACACTTCCATT+AGG | 0.503380 | 1.2:+59307394 | None:intergenic |
| TTCAGAGCTCAAGCTGATGA+AGG | 0.504671 | 1.2:-59307931 | MS.gene40775:CDS |
| ATTTGGCAAGGGCATGTAGC+AGG | 0.507631 | 1.2:-59308399 | MS.gene40775:CDS |
| TCAGCGATCTCACGCGTACC+TGG | 0.508919 | 1.2:-59307226 | MS.gene40775:CDS |
| CAGAAAGGACTCAAGGAGGT+TGG | 0.513664 | 1.2:-59305206 | MS.gene40775:CDS |
| AGATTCAATCCCGTGGTTGG+TGG | 0.514269 | 1.2:-59307421 | MS.gene40775:CDS |
| AATGCAGGTACCAAGGCATT+GGG | 0.515702 | 1.2:-59302449 | MS.gene40775:intron |
| TACATCAGCATATACGGAAC+TGG | 0.519360 | 1.2:-59308036 | MS.gene40775:CDS |
| TGAAGGTATGCTAGCTGCAA+AGG | 0.521850 | 1.2:-59307689 | MS.gene40775:CDS |
| GAAGCTACCACAAGGTGGCC+AGG | 0.525969 | 1.2:+59307208 | None:intergenic |
| CTGGACAATTGAAAGAAGAT+GGG | 0.528669 | 1.2:+59303886 | None:intergenic |
| CCAGGCTACCTATAGAAGTT+GGG | 0.533663 | 1.2:+59305141 | None:intergenic |
| CTTACAACACCTGGCTTCAG+AGG | 0.541034 | 1.2:+59304943 | None:intergenic |
| CAGCTAGCATACCTTCAAAA+GGG | 0.544862 | 1.2:+59307695 | None:intergenic |
| TGCACATGGACAGTATCAGC+TGG | 0.550753 | 1.2:+59301194 | None:intergenic |
| TTTATACCCATCAGCTGCGA+TGG | 0.553980 | 1.2:-59307965 | MS.gene40775:CDS |
| TATTCCTCACTAGATATGCC+TGG | 0.555430 | 1.2:+59309445 | None:intergenic |
| CGTGGGTGAGATGCAGGGGT+TGG | 0.555991 | 1.2:+59310388 | None:intergenic |
| TCTAGGATGTGATTTGGCAA+GGG | 0.563003 | 1.2:-59308410 | MS.gene40775:intron |
| AGGCATTGGGATAAGGATTG+AGG | 0.564277 | 1.2:-59302436 | MS.gene40775:CDS |
| TACCCGTGGGTGAGATGCAG+GGG | 0.566804 | 1.2:+59310384 | None:intergenic |
| CCGACAAATACACAACCATT+CGG | 0.567790 | 1.2:-59306196 | MS.gene40775:intron |
| AGTTGGGTTCAGCTTATGGT+AGG | 0.568189 | 1.2:+59305157 | None:intergenic |
| ATGGCTGAAGTTGTTTAGCA+AGG | 0.569030 | 1.2:+59301257 | None:intergenic |
| CAACCCCTGCATCTCACCCA+CGG | 0.570722 | 1.2:-59310387 | MS.gene40775:CDS |
| GGGAAAGCACTTACAACACC+TGG | 0.570813 | 1.2:+59304934 | None:intergenic |
| ATTAGGCCCACCACCAACCA+CGG | 0.575070 | 1.2:+59307411 | None:intergenic |
| CAATCCTTATCCCAATGCCT+TGG | 0.576534 | 1.2:+59302439 | None:intergenic |
| TTGCTAAACAACTTCAGCCA+TGG | 0.577584 | 1.2:-59301255 | MS.gene40775:CDS |
| TCAATCCCGTGGTTGGTGGT+GGG | 0.577700 | 1.2:-59307417 | MS.gene40775:CDS |
| TGAGAGGTGCGCAGCGAATG+GGG | 0.579429 | 1.2:-59307648 | MS.gene40775:intron |
| AACCCCTGCATCTCACCCAC+GGG | 0.579955 | 1.2:-59310386 | MS.gene40775:intron |
| CAACTTCAGCCATGGAATAG+GGG | 0.581494 | 1.2:-59301247 | MS.gene40775:CDS |
| GACATTGGATGTGAGTTACA+TGG | 0.582230 | 1.2:-59307254 | MS.gene40775:CDS |
| GAAGATGGGATGTACACTCC+TGG | 0.582722 | 1.2:+59303900 | None:intergenic |
| TGATAGCCAAACTCTTCTCT+GGG | 0.583126 | 1.2:+59309389 | None:intergenic |
| TCTCATTGGATACGCCTCGT+TGG | 0.585869 | 1.2:+59308347 | None:intergenic |
| AAAGATGGGGAGATCACACC+AGG | 0.587344 | 1.2:-59309463 | MS.gene40775:CDS |
| ATCAGATTCAATCCCGTGGT+TGG | 0.587531 | 1.2:-59307424 | MS.gene40775:intron |
| TTAGGCCCACCACCAACCAC+GGG | 0.589559 | 1.2:+59307412 | None:intergenic |
| AGCTTACTGTAACAACGTAA+AGG | 0.590674 | 1.2:-59307997 | MS.gene40775:CDS |
| CTGCCAACAACCTGGTGGTG+TGG | 0.593769 | 1.2:-59309284 | MS.gene40775:CDS |
| ATTACAGGCTGCCAACAACC+TGG | 0.596169 | 1.2:-59309292 | MS.gene40775:CDS |
| CAGGTTGAAATGCTGCAGAA+AGG | 0.597743 | 1.2:-59305221 | MS.gene40775:CDS |
| GTACCCGTGGGTGAGATGCA+GGG | 0.598083 | 1.2:+59310383 | None:intergenic |
| ACGCGTACCTGGCCACCTTG+TGG | 0.599158 | 1.2:-59307215 | MS.gene40775:CDS |
| CAGGTAATAACTATTGAACC+AGG | 0.600635 | 1.2:-59303918 | MS.gene40775:CDS |
| TCCAGGTCACTATCTAGGAA+TGG | 0.601316 | 1.2:-59305001 | MS.gene40775:intron |
| AGAAACTAACAAGCATTGTG+TGG | 0.607256 | 1.2:-59306244 | MS.gene40775:CDS |
| TCGTTCTTATTCCAATCACA+AGG | 0.608028 | 1.2:-59310431 | MS.gene40775:CDS |
| GGTTGGTTGTCCAACATCAA+CGG | 0.613022 | 1.2:+59310405 | None:intergenic |
| ATCCTTCCAGATATGATAAG+GGG | 0.625213 | 1.2:-59308092 | MS.gene40775:CDS |
| AATGCTGCAGAAAGGACTCA+AGG | 0.627439 | 1.2:-59305213 | MS.gene40775:CDS |
| TTATTCTAGGAACGATCAGA+AGG | 0.629437 | 1.2:-59307374 | MS.gene40775:intron |
| CTGTATCAGATTCAATCCCG+TGG | 0.633552 | 1.2:-59307428 | MS.gene40775:intron |
| CTTCTTTCAATTGTCCAGAG+AGG | 0.638940 | 1.2:-59303881 | MS.gene40775:intron |
| ATTGTAGCTTCTAAAAGATG+GGG | 0.641791 | 1.2:-59309476 | MS.gene40775:intron |
| ATTTGTCGGATACTAGCACC+AGG | 0.642966 | 1.2:+59306210 | None:intergenic |
| AACCCCTTATCATATCTGGA+AGG | 0.645748 | 1.2:+59308090 | None:intergenic |
| GATCTCTAATGCAGGTACCA+AGG | 0.651356 | 1.2:-59302456 | MS.gene40775:intron |
| ATCAGCATATACGGAACTGG+AGG | 0.655539 | 1.2:-59308033 | MS.gene40775:CDS |
| CACACCAGGCATATCTAGTG+AGG | 0.673963 | 1.2:-59309449 | MS.gene40775:CDS |
| ATCTGTCATCATCTTTACTG+GGG | 0.686632 | 1.2:+59309357 | None:intergenic |
| ACATTGGATGTGAGTTACAT+GGG | 0.693361 | 1.2:-59307253 | MS.gene40775:CDS |
| GAATACGAATGCAAAATGAG+AGG | 0.696219 | 1.2:-59307664 | MS.gene40775:CDS |
| GCAGAAGAGAAGCTACCACA+AGG | 0.709712 | 1.2:+59307200 | None:intergenic |
| GAAGAGAAGCTACCACAAGG+TGG | 0.733869 | 1.2:+59307203 | None:intergenic |
| TCCAATGAGAAAGTTGAACG+AGG | 0.743293 | 1.2:-59308334 | MS.gene40775:intron |
| ACAGGCTGCCAACAACCTGG+TGG | 0.744058 | 1.2:-59309289 | MS.gene40775:CDS |
| GCTGCAGAAAGGACTCAAGG+AGG | 0.745589 | 1.2:-59305210 | MS.gene40775:CDS |
| GAATACATTCAAGGCCAACG+AGG | 0.787952 | 1.2:-59308361 | MS.gene40775:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 59301209 | 59310630 | 59301209 | ID=MS.gene40775 |
| chr1.2 | mRNA | 59301209 | 59310630 | 59301209 | ID=MS.gene40775.t1;Parent=MS.gene40775 |
| chr1.2 | exon | 59310583 | 59310630 | 59310583 | ID=MS.gene40775.t1.exon1;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59310583 | 59310630 | 59310583 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59310387 | 59310473 | 59310387 | ID=MS.gene40775.t1.exon2;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59310387 | 59310473 | 59310387 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59309228 | 59309491 | 59309228 | ID=MS.gene40775.t1.exon3;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59309228 | 59309491 | 59309228 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59308335 | 59308427 | 59308335 | ID=MS.gene40775.t1.exon4;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59308335 | 59308427 | 59308335 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59307908 | 59308114 | 59307908 | ID=MS.gene40775.t1.exon5;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59307908 | 59308114 | 59307908 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59307649 | 59307752 | 59307649 | ID=MS.gene40775.t1.exon6;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59307649 | 59307752 | 59307649 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59307375 | 59307441 | 59307375 | ID=MS.gene40775.t1.exon7;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59307375 | 59307441 | 59307375 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59307196 | 59307303 | 59307196 | ID=MS.gene40775.t1.exon8;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59307196 | 59307303 | 59307196 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59306197 | 59306289 | 59306197 | ID=MS.gene40775.t1.exon9;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59306197 | 59306289 | 59306197 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59305150 | 59305240 | 59305150 | ID=MS.gene40775.t1.exon10;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59305150 | 59305240 | 59305150 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59304948 | 59305018 | 59304948 | ID=MS.gene40775.t1.exon11;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59304948 | 59305018 | 59304948 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59303882 | 59303937 | 59303882 | ID=MS.gene40775.t1.exon12;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59303882 | 59303937 | 59303882 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59302404 | 59302464 | 59302404 | ID=MS.gene40775.t1.exon13;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59302404 | 59302464 | 59302404 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| chr1.2 | exon | 59301209 | 59301322 | 59301209 | ID=MS.gene40775.t1.exon14;Parent=MS.gene40775.t1 |
| chr1.2 | CDS | 59301209 | 59301322 | 59301209 | ID=cds.MS.gene40775.t1;Parent=MS.gene40775.t1 |
| Gene Sequence |
| Protein sequence |
