AlfalfaGEDB | MS.gene40948

M. sativa cultivar XinJiangDaYe / MS.gene40948

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40948.t1	XP_013461602.1	99.1	106	1	0	1	106	1	106	7.20E-46	193

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40948.t1	Q9ZUZ3	38.8	80	44	1	2	81	17	91	5.5e-10	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40948.t1	A0A072V1B1	99.1	106	1	0	1	106	1	106	5.2e-46	193.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049228	MS.gene40948	0.990792	2.39E-184	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40948.t1	MTR_3g097120	99.057	106	1	0	1	106	1	106	4.65e-77	222
MS.gene40948.t1	MTR_8g094980	69.159	107	26	2	7	106	1	107	2.01e-42	135
MS.gene40948.t1	MTR_3g092220	41.379	87	45	1	4	90	28	108	5.07e-17	70.9
MS.gene40948.t1	MTR_7g118010	40.244	82	43	1	9	90	23	98	8.66e-17	70.1
MS.gene40948.t1	MTR_8g026730	39.024	82	44	1	9	90	27	102	1.32e-16	69.7
MS.gene40948.t1	MTR_4g072220	41.463	82	39	2	2	82	19	92	5.43e-14	62.4
MS.gene40948.t1	MTR_2g043960	39.759	83	42	1	9	91	76	150	6.73e-14	63.9
MS.gene40948.t1	MTR_4g072890	42.857	77	35	2	5	80	22	90	1.10e-13	61.6
MS.gene40948.t1	MTR_4g072300	43.590	78	35	2	4	80	21	90	1.10e-13	61.6
MS.gene40948.t1	MTR_3g084250	42.308	78	36	2	4	80	20	89	1.26e-13	61.6
MS.gene40948.t1	MTR_7g405740	40.230	87	43	2	2	87	59	137	2.32e-13	62.4
MS.gene40948.t1	MTR_4g072940	41.772	79	37	2	5	82	53	123	2.58e-13	61.6
MS.gene40948.t1	MTR_4g072330	43.038	79	36	2	3	80	17	87	3.05e-13	60.5
MS.gene40948.t1	MTR_4g072250	43.590	78	34	2	4	80	21	89	4.43e-13	60.1
MS.gene40948.t1	MTR_4g072260	42.857	77	35	2	5	80	22	90	4.64e-13	60.1
MS.gene40948.t1	MTR_4g072240	42.308	78	36	2	4	80	21	90	5.73e-13	59.7
MS.gene40948.t1	MTR_4g072230	42.857	77	33	2	4	79	21	87	7.60e-13	59.3
MS.gene40948.t1	MTR_4g072570	40.506	79	38	2	3	80	19	89	9.88e-13	59.3
MS.gene40948.t1	MTR_4g072500	45.205	73	31	2	5	76	21	85	1.04e-12	59.3
MS.gene40948.t1	MTR_4g072820	45.333	75	32	2	8	81	19	85	1.44e-12	58.5
MS.gene40948.t1	MTR_8g461420	45.833	72	30	2	5	75	21	84	1.46e-12	58.9
MS.gene40948.t1	MTR_4g072640	44.595	74	32	2	4	76	20	85	1.70e-12	58.5
MS.gene40948.t1	MTR_3g098970	40.000	70	34	1	7	76	51	112	1.76e-12	60.1
MS.gene40948.t1	MTR_4g072870	44.595	74	32	2	4	76	21	86	2.22e-12	58.2
MS.gene40948.t1	MTR_3g084240	43.421	76	34	2	2	76	18	85	2.32e-12	58.2
MS.gene40948.t1	MTR_4g072660	41.333	75	35	2	3	76	14	80	2.35e-12	58.2
MS.gene40948.t1	MTR_4g124750	40.476	84	39	2	9	92	69	141	2.38e-12	59.7
MS.gene40948.t1	MTR_4g072880	41.026	78	37	2	4	80	21	90	2.46e-12	58.2
MS.gene40948.t1	MTR_3g084170	43.421	76	34	2	2	76	18	85	2.82e-12	58.2
MS.gene40948.t1	MTR_4g072480	43.243	74	33	2	4	76	20	85	3.99e-12	57.8
MS.gene40948.t1	MTR_3g084210	45.205	73	31	2	4	75	20	84	4.59e-12	57.4
MS.gene40948.t1	MTR_4g072370	43.590	78	35	2	4	80	18	87	4.67e-12	57.4
MS.gene40948.t1	MTR_3g110045	45.833	72	30	2	5	75	80	143	5.70e-12	58.5
MS.gene40948.t1	MTR_3g084190	43.056	72	32	2	5	75	21	84	8.06e-12	57.0
MS.gene40948.t1	MTR_8g096440	43.750	80	37	1	2	81	38	109	8.35e-12	58.2
MS.gene40948.t1	MTR_4g072600	45.946	74	31	2	4	76	15	80	9.18e-12	56.6
MS.gene40948.t1	MTR_1g061570	45.455	66	26	2	9	74	19	74	9.22e-12	57.4
MS.gene40948.t1	MTR_3g084230	45.833	72	30	2	5	75	21	84	9.28e-12	56.6
MS.gene40948.t1	MTR_3g117630	39.506	81	40	2	3	82	19	91	1.03e-11	56.6
MS.gene40948.t1	MTR_4g072910	49.275	69	26	2	9	76	35	95	1.07e-11	56.6
MS.gene40948.t1	MTR_5g008370	46.377	69	28	2	9	76	25	85	1.37e-11	57.4
MS.gene40948.t1	MTR_4g072730	38.667	75	37	2	3	76	19	85	1.66e-11	56.2
MS.gene40948.t1	MTR_4g072310	41.026	78	37	2	4	80	21	90	1.67e-11	56.2
MS.gene40948.t1	MTR_3g084180	39.241	79	39	2	3	80	19	89	2.13e-11	55.8
MS.gene40948.t1	MTR_3g084150	39.744	78	38	2	3	79	19	88	2.33e-11	55.8
MS.gene40948.t1	MTR_3g110038	39.024	82	41	2	2	82	18	91	2.51e-11	55.5
MS.gene40948.t1	MTR_4g072850	40.506	79	38	2	5	82	23	93	2.54e-11	55.5
MS.gene40948.t1	MTR_4g072530	42.466	73	33	2	5	76	21	85	2.55e-11	55.5
MS.gene40948.t1	MTR_4g072620	40.541	74	35	2	4	76	20	85	3.54e-11	55.1
MS.gene40948.t1	MTR_4g072830	41.026	78	37	2	4	80	18	87	6.19e-11	54.7
MS.gene40948.t1	MTR_4g072560	39.189	74	37	2	9	81	32	98	7.13e-11	54.7
MS.gene40948.t1	MTR_8g096500	43.590	78	34	2	12	89	52	119	7.30e-11	55.8
MS.gene40948.t1	MTR_4g072860	44.595	74	32	2	4	76	21	86	7.80e-11	54.3
MS.gene40948.t1	MTR_7g104940	41.667	72	34	1	10	81	51	114	8.67e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40948.t1	AT5G53590	41.975	81	44	1	9	89	46	123	2.63e-17	72.4
MS.gene40948.t1	AT2G46690	38.947	95	52	2	2	96	17	105	5.14e-17	70.9
MS.gene40948.t1	AT4G00880	41.558	77	39	1	8	84	27	97	7.69e-15	65.5
MS.gene40948.t1	AT3G61900	41.333	75	39	1	9	83	30	99	3.06e-14	64.3
MS.gene40948.t1	AT4G34780	42.857	77	38	2	7	82	26	97	3.54e-13	60.8
MS.gene40948.t1	AT4G22620	41.026	78	41	1	3	80	73	145	1.01e-12	60.8
MS.gene40948.t1	AT4G34790	43.478	69	32	2	9	76	39	101	2.71e-11	55.8
MS.gene40948.t1	AT3G43120	42.466	73	34	1	9	81	79	143	3.05e-11	57.0
MS.gene40948.t1	AT5G20810	44.118	68	30	1	9	76	79	138	3.61e-11	57.0
MS.gene40948.t1	AT4G34800	40.000	75	39	2	3	76	15	84	5.04e-11	55.1
MS.gene40948.t1	AT5G20810	44.118	68	30	1	9	76	79	138	7.34e-11	56.6
MS.gene40948.t1	AT4G12410	37.500	72	40	1	9	80	76	142	9.19e-11	55.8

Find 28 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACCAAGCTTATGATGTTTA+TGG	0.111493	3.2:-80997717	MS.gene40948:CDS
TGCAGTTCAAGTGGGTTTAG+AGG	0.387862	3.2:-80997823	MS.gene40948:CDS
CAAGAAGATGAAAGTGAAGA+AGG	0.391141	3.2:-80997853	MS.gene40948:CDS
TGGTTATGGTTATGTTGGTT+AGG	0.415635	3.2:+80997612	None:intergenic
GACGATGCTGATGGTGGTTA+TGG	0.422891	3.2:+80997598	None:intergenic
GATGGTGGTTATGGTTATGT+TGG	0.451887	3.2:+80997607	None:intergenic
AAATTTCGAGTCAACAAGAA+TGG	0.461541	3.2:+80997556	None:intergenic
GGATGGTAAAGATAAGAGAT+AGG	0.469559	3.2:+80997759	None:intergenic
AAGAAGATGAAAGTGAAGAA+GGG	0.500937	3.2:-80997852	MS.gene40948:CDS
ATGATTTCCTTCATCTTCGA+TGG	0.519321	3.2:-80997653	MS.gene40948:CDS
CAATTCGCCATCGAAGATGA+AGG	0.535691	3.2:+80997646	None:intergenic
AGATGAAAGTGAAGAAGGGA+TGG	0.536875	3.2:-80997848	MS.gene40948:CDS
AGGAAATCATCCACAGAACA+TGG	0.538192	3.2:+80997666	None:intergenic
AGCCATAAACATCATAAGCT+TGG	0.538249	3.2:+80997715	None:intergenic
TGGAAGCTTGAGAGGACCAT+TGG	0.551787	3.2:+80997686	None:intergenic
TCAAGAAGACGCTTGAAAAG+TGG	0.553447	3.2:+80997738	None:intergenic
TCTCAAGCTTCCATGTTCTG+TGG	0.559981	3.2:-80997676	MS.gene40948:CDS
ACAGAACATGGAAGCTTGAG+AGG	0.564027	3.2:+80997678	None:intergenic
GGTTTAGAGGAAGAAGAAGA+AGG	0.564757	3.2:-80997810	MS.gene40948:CDS
GGGATGGCTTGCAGTTCAAG+TGG	0.572241	3.2:-80997832	MS.gene40948:CDS
GAATGGAAAATCAGAGCATG+AGG	0.588224	3.2:+80997573	None:intergenic
GCTTGAGAGGACCATTGGTA+TGG	0.590408	3.2:+80997691	None:intergenic
TTAGAGGAAGAAGAAGAAGG+TGG	0.598907	3.2:-80997807	MS.gene40948:CDS
GGATGGCTTGCAGTTCAAGT+GGG	0.612179	3.2:-80997831	MS.gene40948:CDS
GTTTATGGCTACCATACCAA+TGG	0.626042	3.2:-80997702	MS.gene40948:CDS
GAAGACGCTTGAAAAGTGGA+TGG	0.652650	3.2:+80997742	None:intergenic
CATGAGGAAGACGATGCTGA+TGG	0.669865	3.2:+80997589	None:intergenic
GAGGAAGACGATGCTGATGG+TGG	0.699924	3.2:+80997592	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGATGAAAGTGAAGAA+GGG	-	chr3.2:80997578-80997597	MS.gene40948:CDS	30.0%
	AGCCATAAACATCATAAGCT+TGG	+	chr3.2:80997718-80997737	None:intergenic	35.0%
	ATGATTTCCTTCATCTTCGA+TGG	-	chr3.2:80997777-80997796	MS.gene40948:CDS	35.0%
	CAAGAAGATGAAAGTGAAGA+AGG	-	chr3.2:80997577-80997596	MS.gene40948:CDS	35.0%
	GACCAAGCTTATGATGTTTA+TGG	-	chr3.2:80997713-80997732	MS.gene40948:CDS	35.0%
	GGATGGTAAAGATAAGAGAT+AGG	+	chr3.2:80997674-80997693	None:intergenic	35.0%
!	TGGTTATGGTTATGTTGGTT+AGG	+	chr3.2:80997821-80997840	None:intergenic	35.0%
	AGATGAAAGTGAAGAAGGGA+TGG	-	chr3.2:80997582-80997601	MS.gene40948:CDS	40.0%
	AGGAAATCATCCACAGAACA+TGG	+	chr3.2:80997767-80997786	None:intergenic	40.0%
	GAATGGAAAATCAGAGCATG+AGG	+	chr3.2:80997860-80997879	None:intergenic	40.0%
	GGTTTAGAGGAAGAAGAAGA+AGG	-	chr3.2:80997620-80997639	MS.gene40948:CDS	40.0%
	GTTTATGGCTACCATACCAA+TGG	-	chr3.2:80997728-80997747	MS.gene40948:CDS	40.0%
	TCAAGAAGACGCTTGAAAAG+TGG	+	chr3.2:80997695-80997714	None:intergenic	40.0%
	TTAGAGGAAGAAGAAGAAGG+TGG	-	chr3.2:80997623-80997642	MS.gene40948:CDS	40.0%
!	GATGGTGGTTATGGTTATGT+TGG	+	chr3.2:80997826-80997845	None:intergenic	40.0%
	ACAGAACATGGAAGCTTGAG+AGG	+	chr3.2:80997755-80997774	None:intergenic	45.0%
	CAATTCGCCATCGAAGATGA+AGG	+	chr3.2:80997787-80997806	None:intergenic	45.0%
	GAAGACGCTTGAAAAGTGGA+TGG	+	chr3.2:80997691-80997710	None:intergenic	45.0%
	TCTCAAGCTTCCATGTTCTG+TGG	-	chr3.2:80997754-80997773	MS.gene40948:CDS	45.0%
!	TGCAGTTCAAGTGGGTTTAG+AGG	-	chr3.2:80997607-80997626	MS.gene40948:CDS	45.0%
	CATGAGGAAGACGATGCTGA+TGG	+	chr3.2:80997844-80997863	None:intergenic	50.0%
	GCTTGAGAGGACCATTGGTA+TGG	+	chr3.2:80997742-80997761	None:intergenic	50.0%
	TGGAAGCTTGAGAGGACCAT+TGG	+	chr3.2:80997747-80997766	None:intergenic	50.0%
!	GGATGGCTTGCAGTTCAAGT+GGG	-	chr3.2:80997599-80997618	MS.gene40948:CDS	50.0%
!!	GACGATGCTGATGGTGGTTA+TGG	+	chr3.2:80997835-80997854	None:intergenic	50.0%
	GAGGAAGACGATGCTGATGG+TGG	+	chr3.2:80997841-80997860	None:intergenic	55.0%
!	GGGATGGCTTGCAGTTCAAG+TGG	-	chr3.2:80997598-80997617	MS.gene40948:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	80997566	80997886	80997566	ID=MS.gene40948
chr3.2	mRNA	80997566	80997886	80997566	ID=MS.gene40948.t1;Parent=MS.gene40948
chr3.2	exon	80997566	80997886	80997566	ID=MS.gene40948.t1.exon1;Parent=MS.gene40948.t1
chr3.2	CDS	80997566	80997886	80997566	ID=cds.MS.gene40948.t1;Parent=MS.gene40948.t1

Gene Sequence	>MS.gene40948 ATGCAGGAAGACAAGAAGATGAAAGTGAAGAAGGGATGGCTTGCAGTTCAAGTGGGTTTAGAGGAAGAAGAAGAAGGTGGTTCTTGCTCTCAGAAATTTGTCATACCTATCTCTTATCTTTACCATCCACTTTTCAAGCGTCTTCTTGACCAAGCTTATGATGTTTATGGCTACCATACCAATGGTCCTCTCAAGCTTCCATGTTCTGTGGATGATTTCCTTCATCTTCGATGGCGAATTGAGAAAGAATCTCCTAACCAACATAACCATAACCACCATCAGCATCGTCTTCCTCATGCTCTGATTTTCCATTCTTGTTGA
Protein sequence	>MS.gene40948.t1 MQEDKKMKVKKGWLAVQVGLEEEEEGGSCSQKFVIPISYLYHPLFKRLLDQAYDVYGYHTNGPLKLPCSVDDFLHLRWRIEKESPNQHNHNHHQHRLPHALIFHSC