Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41596.t1 | XP_003599280.1 | 98.2 | 170 | 3 | 0 | 1 | 170 | 1 | 170 | 8.50E-89 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41596.t1 | G7IWW8 | 98.2 | 170 | 3 | 0 | 1 | 170 | 1 | 170 | 6.1e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene01564 | MS.gene41596 | PPI |
| MS.gene54297 | MS.gene41596 | PPI |
| MS.gene41596 | MS.gene002580 | PPI |
| MS.gene004251 | MS.gene41596 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41596.t1 | MTR_3g031140 | 98.235 | 170 | 3 | 0 | 1 | 170 | 1 | 170 | 5.28e-122 | 341 |
| MS.gene41596.t1 | MTR_5g074290 | 65.116 | 172 | 44 | 5 | 1 | 170 | 1 | 158 | 3.87e-60 | 184 |
| MS.gene41596.t1 | MTR_2g016150 | 43.333 | 180 | 91 | 3 | 1 | 169 | 1 | 180 | 2.00e-33 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41596.t1 | AT2G30230 | 55.435 | 184 | 59 | 3 | 1 | 170 | 1 | 175 | 1.73e-58 | 181 |
| MS.gene41596.t1 | AT1G06980 | 57.303 | 178 | 57 | 3 | 1 | 170 | 1 | 167 | 1.83e-56 | 175 |
| MS.gene41596.t1 | AT1G29195 | 43.915 | 189 | 85 | 5 | 1 | 169 | 1 | 188 | 5.49e-45 | 147 |
Find 42 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAACAACGCCTTGAGAACT+TGG | 0.264820 | 3.1:-50000574 | None:intergenic |
| AGAAAGGTCGTGTTGGAATA+TGG | 0.306669 | 3.1:+50000872 | MS.gene41596:CDS |
| AACATCACCCTTGCAATATA+AGG | 0.321491 | 3.1:+50000822 | MS.gene41596:CDS |
| TGGCTTGTGTTCTTCCTTGT+GGG | 0.333742 | 3.1:+50000440 | MS.gene41596:CDS |
| TAATAGCTTAAGGTGTTGTT+TGG | 0.366641 | 3.1:+50000420 | MS.gene41596:CDS |
| GGTTTGCTTAGAACATGGTT+AGG | 0.379565 | 3.1:-50000553 | None:intergenic |
| AACCATTCAAATGCACTATT+CGG | 0.407982 | 3.1:-50000477 | None:intergenic |
| ATAATATGGGTAATAGCTTA+AGG | 0.422550 | 3.1:+50000410 | None:intergenic |
| GATCCCGGCAACATCTTTGC+CGG | 0.428218 | 3.1:+50000654 | MS.gene41596:CDS |
| GTTTGCTTAGAACATGGTTA+GGG | 0.435444 | 3.1:-50000552 | None:intergenic |
| GAACATGGTTAGGGTTTGCT+TGG | 0.435589 | 3.1:-50000543 | None:intergenic |
| CATCTTTGCCGGAGAACAAA+AGG | 0.452972 | 3.1:+50000665 | MS.gene41596:CDS |
| TTCTCCGGCAAAGATGTTGC+CGG | 0.464422 | 3.1:-50000658 | None:intergenic |
| TCAAGACGGGATCGCAAGAA+AGG | 0.467249 | 3.1:+50000856 | MS.gene41596:CDS |
| GATGGTGATCATGACAATAA+TGG | 0.475725 | 3.1:+50000769 | MS.gene41596:CDS |
| TTTCTTCTCCTTATATTGCA+AGG | 0.484846 | 3.1:-50000830 | None:intergenic |
| TTGGCTTGTGTTCTTCCTTG+TGG | 0.486689 | 3.1:+50000439 | MS.gene41596:CDS |
| ATCACCATCATCACCGTGCT+TGG | 0.494544 | 3.1:-50000755 | None:intergenic |
| GTTCTCAAGGCGTTGTTCGT+CGG | 0.495768 | 3.1:+50000578 | MS.gene41596:CDS |
| ATCACCAAGCACGGTGATGA+TGG | 0.505783 | 3.1:+50000751 | MS.gene41596:CDS |
| TTCTTCTCCTTATATTGCAA+GGG | 0.509390 | 3.1:-50000829 | None:intergenic |
| CCTCTCTTGAGCTCAGTCTC+CGG | 0.511219 | 3.1:-50000616 | None:intergenic |
| GGCTTGTGTTCTTCCTTGTG+GGG | 0.520338 | 3.1:+50000441 | MS.gene41596:CDS |
| GACAATAATGGTAGTGGTGA+TGG | 0.523601 | 3.1:+50000781 | MS.gene41596:CDS |
| ATCCGAATAGTGCATTTGAA+TGG | 0.531644 | 3.1:+50000475 | MS.gene41596:CDS |
| TCTCCGGCAAAGATGTTGCC+GGG | 0.532431 | 3.1:-50000657 | None:intergenic |
| GGAGAAGAAATCCTCAAGAC+GGG | 0.545214 | 3.1:+50000843 | MS.gene41596:CDS |
| TTTCTTGCGATCCCGTCTTG+AGG | 0.547201 | 3.1:-50000854 | None:intergenic |
| TCAGAAATACTTGCTAAATG+AGG | 0.557315 | 3.1:-50000898 | None:intergenic |
| TTGCCGGAGAACAAAAGGAA+TGG | 0.573260 | 3.1:+50000670 | MS.gene41596:CDS |
| CTAAGCAAACCAAGTTCTCA+AGG | 0.584166 | 3.1:+50000565 | MS.gene41596:CDS |
| AAGCATATACTTCTTGATCC+CGG | 0.589215 | 3.1:+50000639 | MS.gene41596:CDS |
| AGGAGAAGAAATCCTCAAGA+CGG | 0.590285 | 3.1:+50000842 | MS.gene41596:CDS |
| AACTTGGTTTGCTTAGAACA+TGG | 0.593833 | 3.1:-50000558 | None:intergenic |
| GATCATGACAATAATGGTAG+TGG | 0.600345 | 3.1:+50000775 | MS.gene41596:CDS |
| GATCGCAAGAAAGGTCGTGT+TGG | 0.602795 | 3.1:+50000865 | MS.gene41596:CDS |
| CCGGAGACTGAGCTCAAGAG+AGG | 0.605430 | 3.1:+50000616 | MS.gene41596:CDS |
| ATTACACATTCAATCACAGC+AGG | 0.608213 | 3.1:+50000511 | MS.gene41596:CDS |
| AAGAAAAGAATCACCAAGCA+CGG | 0.612290 | 3.1:+50000742 | MS.gene41596:CDS |
| ATCAAATCTAGTGCCCCACA+AGG | 0.641179 | 3.1:-50000454 | None:intergenic |
| ACATTCAATCACAGCAGGTG+AGG | 0.650445 | 3.1:+50000516 | MS.gene41596:CDS |
| AAGGTCGTGTTGGAATATGG+AGG | 0.705859 | 3.1:+50000875 | MS.gene41596:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCATTCAAATGCACTATT+CGG | - | chr3.1:50000480-50000499 | None:intergenic | 30.0% | |
| TTCTTCTCCTTATATTGCAA+GGG | - | chr3.1:50000832-50000851 | None:intergenic | 30.0% | |
| TTTCTTCTCCTTATATTGCA+AGG | - | chr3.1:50000833-50000852 | None:intergenic | 30.0% | |
| ! | TCAGAAATACTTGCTAAATG+AGG | - | chr3.1:50000901-50000920 | None:intergenic | 30.0% |
| !! | TAATAGCTTAAGGTGTTGTT+TGG | + | chr3.1:50000420-50000439 | MS.gene41596:CDS | 30.0% |
| AACATCACCCTTGCAATATA+AGG | + | chr3.1:50000822-50000841 | MS.gene41596:CDS | 35.0% | |
| AAGAAAAGAATCACCAAGCA+CGG | + | chr3.1:50000742-50000761 | MS.gene41596:CDS | 35.0% | |
| AAGCATATACTTCTTGATCC+CGG | + | chr3.1:50000639-50000658 | MS.gene41596:CDS | 35.0% | |
| ATCCGAATAGTGCATTTGAA+TGG | + | chr3.1:50000475-50000494 | MS.gene41596:CDS | 35.0% | |
| ATTACACATTCAATCACAGC+AGG | + | chr3.1:50000511-50000530 | MS.gene41596:CDS | 35.0% | |
| GATCATGACAATAATGGTAG+TGG | + | chr3.1:50000775-50000794 | MS.gene41596:CDS | 35.0% | |
| GATGGTGATCATGACAATAA+TGG | + | chr3.1:50000769-50000788 | MS.gene41596:CDS | 35.0% | |
| GTTTGCTTAGAACATGGTTA+GGG | - | chr3.1:50000555-50000574 | None:intergenic | 35.0% | |
| !! | AACTTGGTTTGCTTAGAACA+TGG | - | chr3.1:50000561-50000580 | None:intergenic | 35.0% |
| !! | TCGGATTTTGATCATTTCAC+CGG | + | chr3.1:50000597-50000616 | MS.gene41596:CDS | 35.0% |
| AGAAAGGTCGTGTTGGAATA+TGG | + | chr3.1:50000872-50000891 | MS.gene41596:CDS | 40.0% | |
| AGGAGAAGAAATCCTCAAGA+CGG | + | chr3.1:50000842-50000861 | MS.gene41596:CDS | 40.0% | |
| CTAAGCAAACCAAGTTCTCA+AGG | + | chr3.1:50000565-50000584 | MS.gene41596:CDS | 40.0% | |
| ! | GATCCATTCCTTTTGTTCTC+CGG | - | chr3.1:50000676-50000695 | None:intergenic | 40.0% |
| ! | GGTTTGCTTAGAACATGGTT+AGG | - | chr3.1:50000556-50000575 | None:intergenic | 40.0% |
| !! | GACAATAATGGTAGTGGTGA+TGG | + | chr3.1:50000781-50000800 | MS.gene41596:CDS | 40.0% |
| AAGGTCGTGTTGGAATATGG+AGG | + | chr3.1:50000875-50000894 | MS.gene41596:CDS | 45.0% | |
| ACATTCAATCACAGCAGGTG+AGG | + | chr3.1:50000516-50000535 | MS.gene41596:CDS | 45.0% | |
| ATCAAATCTAGTGCCCCACA+AGG | - | chr3.1:50000457-50000476 | None:intergenic | 45.0% | |
| CATCTTTGCCGGAGAACAAA+AGG | + | chr3.1:50000665-50000684 | MS.gene41596:CDS | 45.0% | |
| GGAGAAGAAATCCTCAAGAC+GGG | + | chr3.1:50000843-50000862 | MS.gene41596:CDS | 45.0% | |
| TTGCCGGAGAACAAAAGGAA+TGG | + | chr3.1:50000670-50000689 | MS.gene41596:CDS | 45.0% | |
| ! | GAACATGGTTAGGGTTTGCT+TGG | - | chr3.1:50000546-50000565 | None:intergenic | 45.0% |
| ! | TGGCTTGTGTTCTTCCTTGT+GGG | + | chr3.1:50000440-50000459 | MS.gene41596:CDS | 45.0% |
| !! | TTGGCTTGTGTTCTTCCTTG+TGG | + | chr3.1:50000439-50000458 | MS.gene41596:CDS | 45.0% |
| ATCACCAAGCACGGTGATGA+TGG | + | chr3.1:50000751-50000770 | MS.gene41596:CDS | 50.0% | |
| ATCACCATCATCACCGTGCT+TGG | - | chr3.1:50000758-50000777 | None:intergenic | 50.0% | |
| CGAACAACGCCTTGAGAACT+TGG | - | chr3.1:50000577-50000596 | None:intergenic | 50.0% | |
| GATCGCAAGAAAGGTCGTGT+TGG | + | chr3.1:50000865-50000884 | MS.gene41596:CDS | 50.0% | |
| GGCTTGTGTTCTTCCTTGTG+GGG | + | chr3.1:50000441-50000460 | MS.gene41596:CDS | 50.0% | |
| TCAAGACGGGATCGCAAGAA+AGG | + | chr3.1:50000856-50000875 | MS.gene41596:CDS | 50.0% | |
| TTCTCCGGCAAAGATGTTGC+CGG | - | chr3.1:50000661-50000680 | None:intergenic | 50.0% | |
| TTTCTTGCGATCCCGTCTTG+AGG | - | chr3.1:50000857-50000876 | None:intergenic | 50.0% | |
| !! | GTTCTCAAGGCGTTGTTCGT+CGG | + | chr3.1:50000578-50000597 | MS.gene41596:CDS | 50.0% |
| CCTCTCTTGAGCTCAGTCTC+CGG | - | chr3.1:50000619-50000638 | None:intergenic | 55.0% | |
| GATCCCGGCAACATCTTTGC+CGG | + | chr3.1:50000654-50000673 | MS.gene41596:CDS | 55.0% | |
| TCTCCGGCAAAGATGTTGCC+GGG | - | chr3.1:50000660-50000679 | None:intergenic | 55.0% | |
| CCGGAGACTGAGCTCAAGAG+AGG | + | chr3.1:50000616-50000635 | MS.gene41596:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 50000415 | 50000927 | 50000415 | ID=MS.gene41596 |
| chr3.1 | mRNA | 50000415 | 50000927 | 50000415 | ID=MS.gene41596.t1;Parent=MS.gene41596 |
| chr3.1 | exon | 50000415 | 50000927 | 50000415 | ID=MS.gene41596.t1.exon1;Parent=MS.gene41596.t1 |
| chr3.1 | CDS | 50000415 | 50000927 | 50000415 | ID=cds.MS.gene41596.t1;Parent=MS.gene41596.t1 |
| Gene Sequence |
| Protein sequence |
