Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41674.t1 | XP_003602781.1 | 98.4 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 2.20E-214 | 754.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41674.t1 | Q0E0Y3 | 69.0 | 377 | 106 | 3 | 3 | 369 | 8 | 383 | 3.8e-151 | 535.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41674.t1 | G7J3D1 | 98.4 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 1.6e-214 | 754.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055517 | MS.gene41674 | 0.838249 | 3.22E-57 | -1.69E-46 |
| MS.gene056403 | MS.gene41674 | 0.818297 | 2.11E-52 | -1.69E-46 |
| MS.gene056605 | MS.gene41674 | 0.808617 | 2.84E-50 | -1.69E-46 |
| MS.gene060830 | MS.gene41674 | 0.817056 | 4.02E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41674 | MS.gene07153 | PPI |
| MS.gene02998 | MS.gene41674 | PPI |
| MS.gene41674 | MS.gene005659 | PPI |
| MS.gene21545 | MS.gene41674 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41674.t1 | MTR_3g098940 | 98.374 | 369 | 6 | 0 | 1 | 369 | 12 | 380 | 0.0 | 757 |
| MS.gene41674.t1 | MTR_3g098940 | 98.225 | 338 | 6 | 0 | 1 | 338 | 12 | 349 | 0.0 | 686 |
| MS.gene41674.t1 | MTR_1g095580 | 26.616 | 263 | 163 | 7 | 42 | 275 | 29 | 290 | 1.15e-16 | 80.5 |
| MS.gene41674.t1 | MTR_1g095580 | 26.616 | 263 | 163 | 7 | 42 | 275 | 29 | 290 | 1.96e-16 | 79.7 |
| MS.gene41674.t1 | MTR_1g095580 | 25.490 | 306 | 185 | 9 | 42 | 313 | 29 | 325 | 4.51e-16 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41674.t1 | AT5G51140 | 68.785 | 362 | 93 | 4 | 16 | 369 | 46 | 395 | 3.51e-179 | 503 |
| MS.gene41674.t1 | AT5G51140 | 68.555 | 353 | 91 | 4 | 16 | 360 | 46 | 386 | 3.06e-172 | 486 |
| MS.gene41674.t1 | AT3G52260 | 25.000 | 300 | 193 | 7 | 45 | 313 | 34 | 332 | 7.08e-18 | 84.3 |
| MS.gene41674.t1 | AT3G52260 | 26.136 | 264 | 164 | 6 | 45 | 277 | 34 | 297 | 1.97e-17 | 82.8 |
| MS.gene41674.t1 | AT3G52260 | 25.581 | 258 | 161 | 6 | 45 | 271 | 34 | 291 | 3.50e-15 | 75.9 |
| MS.gene41674.t1 | AT3G52260 | 25.984 | 254 | 151 | 6 | 71 | 294 | 91 | 337 | 9.42e-13 | 69.3 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCCCTTCGGTGCCAAATT+TGG | 0.184524 | 3.3:+81491094 | None:intergenic |
| CGCGTTCATTTACAGTCTTC+TGG | 0.226994 | 3.3:-81491310 | MS.gene41674:CDS |
| TAGCATTTCTGGCTATAATA+AGG | 0.255944 | 3.3:+81494976 | None:intergenic |
| GTGTACCAACTGTCCAAATT+TGG | 0.306598 | 3.3:-81491107 | MS.gene41674:CDS |
| AGTTTCGCCCAATCAGGATA+TGG | 0.308758 | 3.3:+81490440 | None:intergenic |
| TTATTCGCTGAGGAATTTAA+AGG | 0.319656 | 3.3:-81497129 | MS.gene41674:CDS |
| TCGAGCATTATAGTCTATAT+TGG | 0.345385 | 3.3:+81493211 | None:intergenic |
| GCTTCTTTGACATCAAATTT+CGG | 0.348641 | 3.3:-81491190 | MS.gene41674:CDS |
| TGCGGTAAAGGAGGATTTGC+TGG | 0.374155 | 3.3:+81497441 | None:intergenic |
| AATATATTGCAAAAGTAGTT+GGG | 0.377867 | 3.3:-81494088 | MS.gene41674:CDS |
| TACAGCATGTCATTGGCTAT+TGG | 0.388217 | 3.3:+81491283 | None:intergenic |
| GAGTACCATTGCTACTAATC+CGG | 0.396097 | 3.3:+81491918 | None:intergenic |
| CAATATATTGCAAAAGTAGT+TGG | 0.398952 | 3.3:-81494089 | MS.gene41674:CDS |
| CCAGCCCATGTTCTGCTTGA+AGG | 0.399831 | 3.3:+81495317 | None:intergenic |
| ACTGTTGATAGATCTATTAA+TGG | 0.400312 | 3.3:-81491247 | MS.gene41674:CDS |
| ATGAATGCCCATATCCTGAT+TGG | 0.401822 | 3.3:-81490448 | MS.gene41674:CDS |
| ATCAATGCTAAACTCCTCAT+TGG | 0.413066 | 3.3:+81491135 | None:intergenic |
| TTACAAACAGTTAGCACATC+TGG | 0.415839 | 3.3:+81496706 | None:intergenic |
| AGCTAAAGTTTCGCCCAATC+AGG | 0.416919 | 3.3:+81490434 | None:intergenic |
| ACGATGTAATCTTGCGGTAA+AGG | 0.424137 | 3.3:+81497429 | None:intergenic |
| TCTGGTTCTGTATGAAGAAT+AGG | 0.424198 | 3.3:+81496724 | None:intergenic |
| ACAGCATGTCATTGGCTATT+GGG | 0.433925 | 3.3:+81491284 | None:intergenic |
| TATCGCAAGAACACTGTTGT+TGG | 0.444093 | 3.3:-81495343 | MS.gene41674:CDS |
| AAGGTCAGAGACTCTACAAA+AGG | 0.453239 | 3.3:-81491965 | MS.gene41674:CDS |
| CGGTGCCAAATTTGGACAGT+TGG | 0.457022 | 3.3:+81491102 | None:intergenic |
| AGTCACTGGCCGCACTCATC+AGG | 0.471463 | 3.3:-81491873 | MS.gene41674:intron |
| GATTTAACTACGTATGAAAC+CGG | 0.475395 | 3.3:+81496881 | None:intergenic |
| CTGTCCAAATTTGGCACCGA+AGG | 0.478848 | 3.3:-81491098 | MS.gene41674:CDS |
| CTGTTGATAGATCTATTAAT+GGG | 0.482702 | 3.3:-81491246 | MS.gene41674:CDS |
| CCTTCAAGCAGAACATGGGC+TGG | 0.487356 | 3.3:-81495317 | MS.gene41674:CDS |
| AAACTTACATAATATTCATA+AGG | 0.492762 | 3.3:+81497102 | None:intergenic |
| CGACTGGATCGCCTTGTCTC+CGG | 0.493010 | 3.3:-81495002 | MS.gene41674:CDS |
| TGATGAGTGCGGCCAGTGAC+TGG | 0.494032 | 3.3:+81491875 | None:intergenic |
| TGAGATGTACAGCATGTCAT+TGG | 0.495292 | 3.3:+81491276 | None:intergenic |
| TAAAGAATTGCATACCCCTT+CGG | 0.504219 | 3.3:+81491082 | None:intergenic |
| GCGATGAATTCGAAGTAGTA+TGG | 0.504359 | 3.3:+81497292 | None:intergenic |
| GTTTCGCCCAATCAGGATAT+GGG | 0.504686 | 3.3:+81490441 | None:intergenic |
| TGAATGCCCATATCCTGATT+GGG | 0.506186 | 3.3:-81490447 | MS.gene41674:CDS |
| TTACATTGTATGCGGTACTC+TGG | 0.510906 | 3.3:-81490485 | MS.gene41674:CDS |
| CAAATAAAATACCTCATCCT+CGG | 0.512397 | 3.3:+81494058 | None:intergenic |
| AAATAAAATACCTCATCCTC+GGG | 0.514851 | 3.3:+81494059 | None:intergenic |
| GGCATCCTTCAAGCAGAACA+TGG | 0.516228 | 3.3:-81495322 | MS.gene41674:CDS |
| TGTCTCAGCTGTCCATCGAC+TGG | 0.517338 | 3.3:-81495018 | MS.gene41674:intron |
| TGGGGAATTTCCCGAGGATG+AGG | 0.518507 | 3.3:-81494069 | MS.gene41674:intron |
| CAGGCATGAACCACCTGTGA+TGG | 0.518717 | 3.3:-81496758 | MS.gene41674:CDS |
| TCTGGCTATAATAAGGAGTC+CGG | 0.521448 | 3.3:+81494983 | None:intergenic |
| AGTACCATTGCTACTAATCC+GGG | 0.522821 | 3.3:+81491919 | None:intergenic |
| TGGTTGTTACAGATTGAGGC+TGG | 0.531299 | 3.3:-81494125 | MS.gene41674:intron |
| AGATATGATACTGATGAAGA+AGG | 0.536074 | 3.3:-81490515 | MS.gene41674:CDS |
| GGGCATTCATATGTCCATCC+AGG | 0.555137 | 3.3:+81490461 | None:intergenic |
| TAGGTTAGTGCGGTGAAATG+TGG | 0.563936 | 3.3:-81496932 | MS.gene41674:CDS |
| TGTCCAAATTTGGCACCGAA+GGG | 0.570374 | 3.3:-81491097 | MS.gene41674:CDS |
| AGGTCAGAGACTCTACAAAA+GGG | 0.571744 | 3.3:-81491964 | MS.gene41674:CDS |
| TGTGGAAGGATTCAAGTTGA+CGG | 0.572120 | 3.3:-81496914 | MS.gene41674:CDS |
| CAAGATTACATCGTTCGAAA+CGG | 0.576471 | 3.3:-81497420 | MS.gene41674:intron |
| TTGCCTCCACAAAATTTACC+CGG | 0.581737 | 3.3:-81491937 | MS.gene41674:CDS |
| TGCGGTACTCTGGACCTGGA+TGG | 0.588015 | 3.3:-81490475 | MS.gene41674:CDS |
| ATACTGATGAAGAAGGTTTG+TGG | 0.590363 | 3.3:-81490508 | MS.gene41674:CDS |
| AGACAAGGCGATCCAGTCGA+TGG | 0.592536 | 3.3:+81495006 | None:intergenic |
| ATAGACTATAATGCTCGAGA+AGG | 0.597554 | 3.3:-81493206 | MS.gene41674:CDS |
| TGTATGCGGTACTCTGGACC+TGG | 0.597884 | 3.3:-81490479 | MS.gene41674:CDS |
| TAATAAGGAGTCCGGAGACA+AGG | 0.599949 | 3.3:+81494991 | None:intergenic |
| TTTACCCGGATTAGTAGCAA+TGG | 0.600058 | 3.3:-81491923 | MS.gene41674:CDS |
| GGAACATCACAAGCCATCAC+AGG | 0.601035 | 3.3:+81496745 | None:intergenic |
| AGAAGGAAGAAGCACAGCAG+AGG | 0.605852 | 3.3:-81493189 | MS.gene41674:intron |
| ATATATTGCAAAAGTAGTTG+GGG | 0.609841 | 3.3:-81494087 | MS.gene41674:CDS |
| TTACTTACAGGGACAGACGC+AGG | 0.614034 | 3.3:+81496682 | None:intergenic |
| TTAGTGCGGTGAAATGTGGA+AGG | 0.615192 | 3.3:-81496928 | MS.gene41674:CDS |
| AGCCAGAAATGCTACAAAAG+CGG | 0.615290 | 3.3:-81494967 | MS.gene41674:CDS |
| ATGTAATCTTGCGGTAAAGG+AGG | 0.615710 | 3.3:+81497432 | None:intergenic |
| TATTGTAGATTTATTCGCTG+AGG | 0.616478 | 3.3:-81497139 | MS.gene41674:CDS |
| TTTGGACAGTTGGTACACAT+AGG | 0.616779 | 3.3:+81491112 | None:intergenic |
| AGTTGACGGTGAAATCGTGC+CGG | 0.620508 | 3.3:-81496900 | MS.gene41674:CDS |
| CAGAGACTCTACAAAAGGGA+AGG | 0.630227 | 3.3:-81491960 | MS.gene41674:CDS |
| GTATGGATCTTAGGTTAGTG+CGG | 0.630653 | 3.3:-81496942 | MS.gene41674:intron |
| GTTTGTGGTTACATTGTATG+CGG | 0.636819 | 3.3:-81490493 | MS.gene41674:CDS |
| GTCCAAATTTGGCACCGAAG+GGG | 0.645456 | 3.3:-81491096 | MS.gene41674:intron |
| GCATCCTTCAAGCAGAACAT+GGG | 0.646483 | 3.3:-81495321 | MS.gene41674:CDS |
| TTCTTGCGATATTGACCACA+TGG | 0.665528 | 3.3:+81495355 | None:intergenic |
| TTGTCAAGAAAATTCCAATG+AGG | 0.670627 | 3.3:-81491149 | MS.gene41674:CDS |
| ACATCACAAGCCATCACAGG+TGG | 0.674685 | 3.3:+81496748 | None:intergenic |
| AGTAGTTGGGGAATTTCCCG+AGG | 0.679238 | 3.3:-81494075 | MS.gene41674:CDS |
| TTTCGAACGATGTAATCTTG+CGG | 0.684194 | 3.3:+81497423 | None:intergenic |
| AAGAGTGTATGGATATCGTG+TGG | 0.692668 | 3.3:-81497470 | None:intergenic |
| GGTACATAGGTTCATCCATG+TGG | 0.702272 | 3.3:-81495370 | MS.gene41674:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 81490436 | 81497484 | 81490436 | ID=MS.gene41674 |
| chr3.3 | mRNA | 81490436 | 81497484 | 81490436 | ID=MS.gene41674.t1;Parent=MS.gene41674 |
| chr3.3 | exon | 81497421 | 81497484 | 81497421 | ID=MS.gene41674.t1.exon1;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81497421 | 81497484 | 81497421 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81497288 | 81497331 | 81497288 | ID=MS.gene41674.t1.exon2;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81497288 | 81497331 | 81497288 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81497110 | 81497187 | 81497110 | ID=MS.gene41674.t1.exon3;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81497110 | 81497187 | 81497110 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81496851 | 81496951 | 81496851 | ID=MS.gene41674.t1.exon4;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81496851 | 81496951 | 81496851 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81496690 | 81496777 | 81496690 | ID=MS.gene41674.t1.exon5;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81496690 | 81496777 | 81496690 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81495305 | 81495383 | 81495305 | ID=MS.gene41674.t1.exon6;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81495305 | 81495383 | 81495305 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81494950 | 81495032 | 81494950 | ID=MS.gene41674.t1.exon7;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81494950 | 81495032 | 81494950 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81494070 | 81494135 | 81494070 | ID=MS.gene41674.t1.exon8;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81494070 | 81494135 | 81494070 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81493190 | 81493246 | 81493190 | ID=MS.gene41674.t1.exon9;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81493190 | 81493246 | 81493190 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81491874 | 81491984 | 81491874 | ID=MS.gene41674.t1.exon10;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81491874 | 81491984 | 81491874 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81491097 | 81491335 | 81491097 | ID=MS.gene41674.t1.exon11;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81491097 | 81491335 | 81491097 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| chr3.3 | exon | 81490436 | 81490535 | 81490436 | ID=MS.gene41674.t1.exon12;Parent=MS.gene41674.t1 |
| chr3.3 | CDS | 81490436 | 81490535 | 81490436 | ID=cds.MS.gene41674.t1;Parent=MS.gene41674.t1 |
| Gene Sequence |
| Protein sequence |
