Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42152.t1 | XP_003624886.2 | 98.5 | 206 | 3 | 0 | 1 | 206 | 358 | 563 | 7.80E-113 | 416.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42152.t1 | A8MS85 | 69.6 | 207 | 56 | 2 | 1 | 206 | 340 | 540 | 6.5e-76 | 285.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42152.t1 | G7L4U0 | 98.5 | 206 | 3 | 0 | 1 | 206 | 358 | 563 | 5.6e-113 | 416.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060349 | MS.gene42152 | 0.802803 | 4.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42152.t1 | MTR_7g088700 | 98.544 | 206 | 3 | 0 | 1 | 206 | 358 | 563 | 2.94e-140 | 428 |
| MS.gene42152.t1 | MTR_7g087460 | 90.777 | 206 | 18 | 1 | 1 | 206 | 309 | 513 | 6.63e-131 | 385 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42152.t1 | AT1G65440 | 69.565 | 207 | 56 | 2 | 1 | 206 | 311 | 511 | 6.85e-92 | 293 |
| MS.gene42152.t1 | AT1G65440 | 69.565 | 207 | 56 | 2 | 1 | 206 | 340 | 540 | 7.13e-92 | 294 |
| MS.gene42152.t1 | AT1G65440 | 69.565 | 207 | 56 | 2 | 1 | 206 | 340 | 540 | 1.54e-91 | 293 |
| MS.gene42152.t1 | AT1G65440 | 69.565 | 207 | 56 | 2 | 1 | 206 | 340 | 540 | 1.54e-91 | 293 |
| MS.gene42152.t1 | AT1G63210 | 59.330 | 209 | 71 | 5 | 1 | 206 | 135 | 332 | 7.79e-66 | 219 |
Find 58 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTCTTCTGGTGTTTCCTT+TGG | 0.245181 | 7.3:+20621142 | None:intergenic |
| TGGGAGGTTGCAAGCAGGTT+TGG | 0.280975 | 7.3:-20621302 | MS.gene42152:CDS |
| TTCCGATACATGGCAATAAA+AGG | 0.283926 | 7.3:+20621837 | None:intergenic |
| GTGCATTCTTTAGATACTTT+GGG | 0.291528 | 7.3:-20621633 | MS.gene42152:intron |
| AAGTTAGAAGCCACCTCTTC+TGG | 0.305574 | 7.3:+20621130 | None:intergenic |
| ACAAGACACCTATTCTAAAA+TGG | 0.310686 | 7.3:-20621743 | MS.gene42152:CDS |
| GCACATTTAAGTACTTCTTC+AGG | 0.322441 | 7.3:+20621085 | None:intergenic |
| GGGAGGTTGCAAGCAGGTTT+GGG | 0.327561 | 7.3:-20621301 | MS.gene42152:CDS |
| CTGAAGCCGGTGATGAGAAT+TGG | 0.333186 | 7.3:-20621776 | MS.gene42152:CDS |
| TGTGCATTCTTTAGATACTT+TGG | 0.349967 | 7.3:-20621634 | MS.gene42152:intron |
| CAATTCTCATCACCGGCTTC+AGG | 0.374054 | 7.3:+20621777 | None:intergenic |
| AAACGAGACTAAACTTGAAT+AGG | 0.385813 | 7.3:-20621502 | MS.gene42152:CDS |
| CACATTTAAGTACTTCTTCA+GGG | 0.432100 | 7.3:+20621086 | None:intergenic |
| GACAGTACAAAAGGCCGAAA+AGG | 0.444752 | 7.3:-20621361 | MS.gene42152:CDS |
| TGAAGCCGGTGATGAGAATT+GGG | 0.456149 | 7.3:-20621775 | MS.gene42152:CDS |
| ACCGTTTGTTGTAGTATGAC+TGG | 0.480839 | 7.3:+20621555 | None:intergenic |
| CACCGGCTTCAGGTCGCTCT+AGG | 0.487429 | 7.3:+20621787 | None:intergenic |
| TCCAGTCATACTACAACAAA+CGG | 0.496704 | 7.3:-20621556 | MS.gene42152:CDS |
| CATTTCCCACCTGGTGAAGC+TGG | 0.499805 | 7.3:-20621395 | MS.gene42152:CDS |
| GTCTGTGGGAGGTTGCAAGC+AGG | 0.514398 | 7.3:-20621307 | MS.gene42152:CDS |
| GGGCTCTACATGACTTGGAC+AGG | 0.518377 | 7.3:-20621613 | MS.gene42152:CDS |
| GACCTAGAGCGACCTGAAGC+CGG | 0.524698 | 7.3:-20621789 | MS.gene42152:CDS |
| TTTCAGTAAGGCGGGTCTGT+GGG | 0.528549 | 7.3:-20621321 | MS.gene42152:CDS |
| AATGTATAGCACTTTCAGTA+AGG | 0.529084 | 7.3:-20621333 | MS.gene42152:CDS |
| ACACTCCTCCTTCCGATACA+TGG | 0.532263 | 7.3:+20621827 | None:intergenic |
| CTTATCCCAATTCTCATCAC+CGG | 0.534435 | 7.3:+20621770 | None:intergenic |
| TACATGACTTGGACAGGAAG+TGG | 0.537671 | 7.3:-20621607 | MS.gene42152:CDS |
| CTTTCAGTAAGGCGGGTCTG+TGG | 0.537705 | 7.3:-20621322 | MS.gene42152:CDS |
| TTTCCTTCTACAGCAAGAGT+TGG | 0.543421 | 7.3:-20621170 | MS.gene42152:intron |
| TGGGAAATGTACATTGAACT+TGG | 0.544645 | 7.3:+20621409 | None:intergenic |
| ATGTCTATCTCTAGTTCAGA+TGG | 0.551835 | 7.3:-20621255 | MS.gene42152:intron |
| TTGATGAAGGACAGTACAAA+AGG | 0.553717 | 7.3:-20621370 | MS.gene42152:CDS |
| ACTTTGGGCTCTACATGACT+TGG | 0.554547 | 7.3:-20621618 | MS.gene42152:CDS |
| TATAGCACTTTCAGTAAGGC+GGG | 0.565845 | 7.3:-20621329 | MS.gene42152:CDS |
| AAGAAGTACTTAAATGTGCT+AGG | 0.566245 | 7.3:-20621081 | MS.gene42152:CDS |
| GCAAGAGTTGGAAGATCCAA+AGG | 0.575325 | 7.3:-20621158 | MS.gene42152:CDS |
| TATTGCCATGTATCGGAAGG+AGG | 0.579629 | 7.3:-20621832 | MS.gene42152:CDS |
| GGAGGCAGAATCAGAGAGGG+AGG | 0.580298 | 7.3:-20621447 | MS.gene42152:CDS |
| CAGTAAGGCGGGTCTGTGGG+AGG | 0.582295 | 7.3:-20621318 | MS.gene42152:CDS |
| GGGTGTAGTTCTGAGCAACT+CGG | 0.582839 | 7.3:-20621281 | MS.gene42152:CDS |
| AGTGGTTGCTTCTTCAGAAG+CGG | 0.585464 | 7.3:-20621589 | MS.gene42152:CDS |
| TCCAAAGGAAACACCAGAAG+AGG | 0.585772 | 7.3:-20621143 | MS.gene42152:CDS |
| TCATCAACACCAGCTTCACC+AGG | 0.590220 | 7.3:+20621386 | None:intergenic |
| AAAGGAAACACCAGAAGAGG+TGG | 0.597490 | 7.3:-20621140 | MS.gene42152:CDS |
| ACATTTAAGTACTTCTTCAG+GGG | 0.601409 | 7.3:+20621087 | None:intergenic |
| ATCAGTTATGAGATCACTGA+AGG | 0.605010 | 7.3:-20621468 | MS.gene42152:CDS |
| TTCAATGTACATTTCCCACC+TGG | 0.606054 | 7.3:-20621404 | MS.gene42152:CDS |
| GGTGAAGCTGGTGTTGATGA+AGG | 0.606416 | 7.3:-20621383 | MS.gene42152:CDS |
| GTATAGCACTTTCAGTAAGG+CGG | 0.614366 | 7.3:-20621330 | MS.gene42152:CDS |
| ACTTAAATGTGCTAGGCACA+TGG | 0.624354 | 7.3:-20621074 | None:intergenic |
| GAAGGAGGCAGAATCAGAGA+GGG | 0.631106 | 7.3:-20621450 | MS.gene42152:CDS |
| AGTTATGAGATCACTGAAGG+AGG | 0.645833 | 7.3:-20621465 | MS.gene42152:CDS |
| GTTTGTTGTAGTATGACTGG+AGG | 0.657669 | 7.3:+20621558 | None:intergenic |
| TCAACACCAGCTTCACCAGG+TGG | 0.666297 | 7.3:+20621389 | None:intergenic |
| ACCTATTCTAAAATGGCACA+AGG | 0.669082 | 7.3:-20621736 | MS.gene42152:intron |
| TTTGTTGTAGTATGACTGGA+GGG | 0.674186 | 7.3:+20621559 | None:intergenic |
| TGAAGGAGGCAGAATCAGAG+AGG | 0.676860 | 7.3:-20621451 | MS.gene42152:CDS |
| CAACACCAGCTTCACCAGGT+GGG | 0.676994 | 7.3:+20621390 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACAAAACCTTTAGTTGA+TGG | + | chr7.3:20621722-20621741 | None:intergenic | 25.0% |
| !!! | TTTTTTTGACCATCAACTAA+AGG | - | chr7.3:20621710-20621729 | MS.gene42152:intron | 25.0% |
| AAACGAGACTAAACTTGAAT+AGG | - | chr7.3:20621413-20621432 | MS.gene42152:CDS | 30.0% | |
| AATGTATAGCACTTTCAGTA+AGG | - | chr7.3:20621582-20621601 | MS.gene42152:CDS | 30.0% | |
| ACATTTAAGTACTTCTTCAG+GGG | + | chr7.3:20621831-20621850 | None:intergenic | 30.0% | |
| CACATTTAAGTACTTCTTCA+GGG | + | chr7.3:20621832-20621851 | None:intergenic | 30.0% | |
| GTGCATTCTTTAGATACTTT+GGG | - | chr7.3:20621282-20621301 | MS.gene42152:CDS | 30.0% | |
| TGTGCATTCTTTAGATACTT+TGG | - | chr7.3:20621281-20621300 | MS.gene42152:CDS | 30.0% | |
| ! | AAAGTATCTAAAGAATGCAC+AGG | + | chr7.3:20621282-20621301 | None:intergenic | 30.0% |
| ! | ACAAGACACCTATTCTAAAA+TGG | - | chr7.3:20621172-20621191 | MS.gene42152:intron | 30.0% |
| ! | TTCCTTTTATTGCCATGTAT+CGG | - | chr7.3:20621076-20621095 | MS.gene42152:CDS | 30.0% |
| !! | AAGAAGTACTTAAATGTGCT+AGG | - | chr7.3:20621834-20621853 | MS.gene42152:CDS | 30.0% |
| !! | GGAGAGTTTTTAGATGTTAT+AGG | + | chr7.3:20621249-20621268 | None:intergenic | 30.0% |
| !!! | CTATACATTGACTTCCTTTT+CGG | + | chr7.3:20621571-20621590 | None:intergenic | 30.0% |
| ATCAGTTATGAGATCACTGA+AGG | - | chr7.3:20621447-20621466 | MS.gene42152:CDS | 35.0% | |
| GCACATTTAAGTACTTCTTC+AGG | + | chr7.3:20621833-20621852 | None:intergenic | 35.0% | |
| TCCAGTCATACTACAACAAA+CGG | - | chr7.3:20621359-20621378 | MS.gene42152:CDS | 35.0% | |
| TGGGAAATGTACATTGAACT+TGG | + | chr7.3:20621509-20621528 | None:intergenic | 35.0% | |
| TTCCGATACATGGCAATAAA+AGG | + | chr7.3:20621081-20621100 | None:intergenic | 35.0% | |
| TTGATGAAGGACAGTACAAA+AGG | - | chr7.3:20621545-20621564 | MS.gene42152:CDS | 35.0% | |
| ! | ATGTCTATCTCTAGTTCAGA+TGG | - | chr7.3:20621660-20621679 | MS.gene42152:intron | 35.0% |
| ! | TTTGTTGTAGTATGACTGGA+GGG | + | chr7.3:20621359-20621378 | None:intergenic | 35.0% |
| ! | TTTTATTGCCATGTATCGGA+AGG | - | chr7.3:20621080-20621099 | MS.gene42152:CDS | 35.0% |
| !! | ACCTATTCTAAAATGGCACA+AGG | - | chr7.3:20621179-20621198 | MS.gene42152:intron | 35.0% |
| !!! | ACCTTGTGCCATTTTAGAAT+AGG | + | chr7.3:20621183-20621202 | None:intergenic | 35.0% |
| AGTTATGAGATCACTGAAGG+AGG | - | chr7.3:20621450-20621469 | MS.gene42152:CDS | 40.0% | |
| CTTATCCCAATTCTCATCAC+CGG | + | chr7.3:20621148-20621167 | None:intergenic | 40.0% | |
| GAATGCACAGGCGATAAAAT+TGG | + | chr7.3:20621270-20621289 | None:intergenic | 40.0% | |
| GTATAGCACTTTCAGTAAGG+CGG | - | chr7.3:20621585-20621604 | MS.gene42152:CDS | 40.0% | |
| TATAGCACTTTCAGTAAGGC+GGG | - | chr7.3:20621586-20621605 | MS.gene42152:CDS | 40.0% | |
| TTCAATGTACATTTCCCACC+TGG | - | chr7.3:20621511-20621530 | MS.gene42152:CDS | 40.0% | |
| TTTCCTTCTACAGCAAGAGT+TGG | - | chr7.3:20621745-20621764 | MS.gene42152:CDS | 40.0% | |
| !! | ACCGTTTGTTGTAGTATGAC+TGG | + | chr7.3:20621363-20621382 | None:intergenic | 40.0% |
| !! | GTTTGTTGTAGTATGACTGG+AGG | + | chr7.3:20621360-20621379 | None:intergenic | 40.0% |
| AAAGGAAACACCAGAAGAGG+TGG | - | chr7.3:20621775-20621794 | MS.gene42152:CDS | 45.0% | |
| AAGTTAGAAGCCACCTCTTC+TGG | + | chr7.3:20621788-20621807 | None:intergenic | 45.0% | |
| ACCTCTTCTGGTGTTTCCTT+TGG | + | chr7.3:20621776-20621795 | None:intergenic | 45.0% | |
| ACTTTGGGCTCTACATGACT+TGG | - | chr7.3:20621297-20621316 | MS.gene42152:CDS | 45.0% | |
| AGTGGTTGCTTCTTCAGAAG+CGG | - | chr7.3:20621326-20621345 | MS.gene42152:CDS | 45.0% | |
| GACAGTACAAAAGGCCGAAA+AGG | - | chr7.3:20621554-20621573 | MS.gene42152:CDS | 45.0% | |
| GCAAGAGTTGGAAGATCCAA+AGG | - | chr7.3:20621757-20621776 | MS.gene42152:CDS | 45.0% | |
| TATTGCCATGTATCGGAAGG+AGG | - | chr7.3:20621083-20621102 | MS.gene42152:CDS | 45.0% | |
| TCCAAAGGAAACACCAGAAG+AGG | - | chr7.3:20621772-20621791 | MS.gene42152:CDS | 45.0% | |
| TGAAGCCGGTGATGAGAATT+GGG | - | chr7.3:20621140-20621159 | MS.gene42152:CDS | 45.0% | |
| ! | CTTCCAACTCTTGCTGTAGA+AGG | + | chr7.3:20621751-20621770 | None:intergenic | 45.0% |
| ! | TACATGACTTGGACAGGAAG+TGG | - | chr7.3:20621308-20621327 | MS.gene42152:CDS | 45.0% |
| ACACTCCTCCTTCCGATACA+TGG | + | chr7.3:20621091-20621110 | None:intergenic | 50.0% | |
| CTGAAGCCGGTGATGAGAAT+TGG | - | chr7.3:20621139-20621158 | MS.gene42152:CDS | 50.0% | |
| GAAGGAGGCAGAATCAGAGA+GGG | - | chr7.3:20621465-20621484 | MS.gene42152:CDS | 50.0% | |
| TCATCAACACCAGCTTCACC+AGG | + | chr7.3:20621532-20621551 | None:intergenic | 50.0% | |
| TGAAGGAGGCAGAATCAGAG+AGG | - | chr7.3:20621464-20621483 | MS.gene42152:CDS | 50.0% | |
| TTTCAGTAAGGCGGGTCTGT+GGG | - | chr7.3:20621594-20621613 | MS.gene42152:CDS | 50.0% | |
| !! | CAATTCTCATCACCGGCTTC+AGG | + | chr7.3:20621141-20621160 | None:intergenic | 50.0% |
| !! | GGGTGTAGTTCTGAGCAACT+CGG | - | chr7.3:20621634-20621653 | MS.gene42152:intron | 50.0% |
| !! | GGTGAAGCTGGTGTTGATGA+AGG | - | chr7.3:20621532-20621551 | MS.gene42152:CDS | 50.0% |
| CAACACCAGCTTCACCAGGT+GGG | + | chr7.3:20621528-20621547 | None:intergenic | 55.0% | |
| CATTTCCCACCTGGTGAAGC+TGG | - | chr7.3:20621520-20621539 | MS.gene42152:CDS | 55.0% | |
| CTTTCAGTAAGGCGGGTCTG+TGG | - | chr7.3:20621593-20621612 | MS.gene42152:CDS | 55.0% | |
| GGGAGGTTGCAAGCAGGTTT+GGG | - | chr7.3:20621614-20621633 | MS.gene42152:CDS | 55.0% | |
| TCAACACCAGCTTCACCAGG+TGG | + | chr7.3:20621529-20621548 | None:intergenic | 55.0% | |
| TGGGAGGTTGCAAGCAGGTT+TGG | - | chr7.3:20621613-20621632 | MS.gene42152:CDS | 55.0% | |
| ! | GGGCTCTACATGACTTGGAC+AGG | - | chr7.3:20621302-20621321 | MS.gene42152:CDS | 55.0% |
| GACCTAGAGCGACCTGAAGC+CGG | - | chr7.3:20621126-20621145 | MS.gene42152:CDS | 60.0% | |
| GGAGGCAGAATCAGAGAGGG+AGG | - | chr7.3:20621468-20621487 | MS.gene42152:CDS | 60.0% | |
| GTCTGTGGGAGGTTGCAAGC+AGG | - | chr7.3:20621608-20621627 | MS.gene42152:CDS | 60.0% | |
| CAGTAAGGCGGGTCTGTGGG+AGG | - | chr7.3:20621597-20621616 | MS.gene42152:CDS | 65.0% | |
| !! | CACCGGCTTCAGGTCGCTCT+AGG | + | chr7.3:20621131-20621150 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 20621075 | 20621862 | 20621075 | ID=MS.gene42152 |
| chr7.3 | mRNA | 20621075 | 20621862 | 20621075 | ID=MS.gene42152.t1;Parent=MS.gene42152 |
| chr7.3 | exon | 20621737 | 20621862 | 20621737 | ID=MS.gene42152.t1.exon1;Parent=MS.gene42152.t1 |
| chr7.3 | CDS | 20621737 | 20621862 | 20621737 | ID=cds.MS.gene42152.t1;Parent=MS.gene42152.t1 |
| chr7.3 | exon | 20621256 | 20621642 | 20621256 | ID=MS.gene42152.t1.exon2;Parent=MS.gene42152.t1 |
| chr7.3 | CDS | 20621256 | 20621642 | 20621256 | ID=cds.MS.gene42152.t1;Parent=MS.gene42152.t1 |
| chr7.3 | exon | 20621075 | 20621179 | 20621075 | ID=MS.gene42152.t1.exon3;Parent=MS.gene42152.t1 |
| chr7.3 | CDS | 20621075 | 20621179 | 20621075 | ID=cds.MS.gene42152.t1;Parent=MS.gene42152.t1 |
| Gene Sequence |
| Protein sequence |
