Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42274.t1 | RHN42280.1 | 87.7 | 163 | 16 | 2 | 8 | 166 | 279 | 441 | 4.40E-74 | 287.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42274.t1 | Q9LV32 | 54.1 | 170 | 74 | 2 | 1 | 166 | 210 | 379 | 9.3e-49 | 194.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42274.t1 | A0A072THJ1 | 87.7 | 163 | 16 | 2 | 8 | 166 | 279 | 441 | 3.2e-74 | 287.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052665 | MS.gene42274 | 0.810162 | 1.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42274.t1 | MTR_0189s0050 | 87.730 | 163 | 16 | 2 | 8 | 166 | 279 | 441 | 1.03e-97 | 291 |
| MS.gene42274.t1 | MTR_3g105990 | 85.890 | 163 | 19 | 2 | 8 | 166 | 281 | 443 | 3.53e-91 | 288 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42274.t1 | AT3G18090 | 54.118 | 170 | 74 | 2 | 1 | 166 | 146 | 315 | 2.02e-59 | 198 |
| MS.gene42274.t1 | AT3G18090 | 54.118 | 170 | 74 | 2 | 1 | 166 | 146 | 315 | 2.22e-59 | 198 |
| MS.gene42274.t1 | AT3G18090 | 54.118 | 170 | 74 | 2 | 1 | 166 | 146 | 315 | 4.52e-59 | 198 |
| MS.gene42274.t1 | AT3G23780 | 54.601 | 163 | 70 | 2 | 8 | 166 | 271 | 433 | 3.51e-57 | 192 |
| MS.gene42274.t1 | AT3G23780 | 54.601 | 163 | 70 | 2 | 8 | 166 | 271 | 433 | 3.51e-57 | 192 |
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATGTCTTTCCGAACATTA+AGG | 0.230507 | 3.2:-90121015 | MS.gene42274:CDS |
| CATGGTGAAGGTTCTCTTAC+TGG | 0.253238 | 3.2:-90120961 | MS.gene42274:CDS |
| ATTGTTGATCTCATCGATTA+TGG | 0.358647 | 3.2:-90121200 | MS.gene42274:CDS |
| GTTCTCTTACTGGCTTATAC+TGG | 0.359333 | 3.2:-90120951 | MS.gene42274:CDS |
| TATGTCTTTCCGAACATTAA+GGG | 0.378939 | 3.2:-90121014 | MS.gene42274:CDS |
| GGGATGACTTTCAAAATAAG+AGG | 0.403829 | 3.2:-90120908 | MS.gene42274:CDS |
| ACGAAATGTATCACATTTGT+CGG | 0.440086 | 3.2:+90121127 | None:intergenic |
| GCACATGCTCGGAAGCGAAT+GGG | 0.446807 | 3.2:-90120837 | MS.gene42274:CDS |
| TCGGAAAGACATACAAGTTA+AGG | 0.463583 | 3.2:+90121024 | None:intergenic |
| AACAATCTCTTTATCTGAAG+TGG | 0.475073 | 3.2:+90121217 | None:intergenic |
| AATAAGAGGCTTGAGTTGAC+TGG | 0.485554 | 3.2:-90120894 | MS.gene42274:CDS |
| GGCACGGTACCTGGCATACA+TGG | 0.508318 | 3.2:-90120979 | MS.gene42274:CDS |
| GCTCTGCAAAGAGACCTCTA+TGG | 0.509833 | 3.2:-90120810 | MS.gene42274:CDS |
| ACATTTGTCGGCTTCACGAA+TGG | 0.519230 | 3.2:+90121139 | None:intergenic |
| TTTCTTAGAGGAACGTGTCA+AGG | 0.521437 | 3.2:-90121084 | MS.gene42274:CDS |
| ATCGATTATGGAGAGGGAGA+TGG | 0.529999 | 3.2:-90121188 | MS.gene42274:CDS |
| TGCACATGCTCGGAAGCGAA+TGG | 0.531358 | 3.2:-90120838 | MS.gene42274:CDS |
| CAAATGTGATACATTTCGTA+GGG | 0.535115 | 3.2:-90121123 | MS.gene42274:CDS |
| GAACCTTCACCATGTATGCC+AGG | 0.538389 | 3.2:+90120970 | None:intergenic |
| TGCTCGGAAGCGAATGGGGA+AGG | 0.548332 | 3.2:-90120832 | MS.gene42274:CDS |
| AAGTGCACTTTGCACATGCT+CGG | 0.551400 | 3.2:-90120848 | MS.gene42274:CDS |
| GCACTCGTCAATAGATTCTG+AGG | 0.566118 | 3.2:+90121046 | None:intergenic |
| GATCTCATCGATTATGGAGA+GGG | 0.573846 | 3.2:-90121194 | MS.gene42274:CDS |
| TTCTTAGAGGAACGTGTCAA+GGG | 0.577874 | 3.2:-90121083 | MS.gene42274:CDS |
| GCATAAAATAGTATCCATAC+CGG | 0.583912 | 3.2:+90121239 | None:intergenic |
| CACATGCTCGGAAGCGAATG+GGG | 0.584920 | 3.2:-90120836 | MS.gene42274:CDS |
| AAATGAAGAGAAGTTGCTCA+CGG | 0.590239 | 3.2:-90121283 | MS.gene42274:CDS |
| ACAAATGTGATACATTTCGT+AGG | 0.596270 | 3.2:-90121124 | MS.gene42274:CDS |
| AAACATCTCAATCTCCATAG+AGG | 0.600669 | 3.2:+90120796 | None:intergenic |
| TGTCTGTTGAGATTCCGGTA+TGG | 0.606023 | 3.2:-90121253 | MS.gene42274:CDS |
| CACTCGTCAATAGATTCTGA+GGG | 0.638441 | 3.2:+90121047 | None:intergenic |
| TGATCTCATCGATTATGGAG+AGG | 0.644956 | 3.2:-90121195 | MS.gene42274:CDS |
| GTACCTGGCATACATGGTGA+AGG | 0.671158 | 3.2:-90120973 | MS.gene42274:CDS |
| TCGTCAATAGATTCTGAGGG+CGG | 0.676515 | 3.2:+90121050 | None:intergenic |
| AAATGTGATACATTTCGTAG+GGG | 0.709049 | 3.2:-90121122 | MS.gene42274:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATTAAGGGATTAAAAAGAA+AGG | - | chr3.2:90121084-90121103 | MS.gene42274:CDS | 25.0% |
| !! | CTTTTTAATCCCTTAATGTT+CGG | + | chr3.2:90121082-90121101 | None:intergenic | 25.0% |
| AAATGTGATACATTTCGTAG+GGG | - | chr3.2:90120962-90120981 | MS.gene42274:CDS | 30.0% | |
| AACAATCTCTTTATCTGAAG+TGG | + | chr3.2:90120870-90120889 | None:intergenic | 30.0% | |
| ACAAATGTGATACATTTCGT+AGG | - | chr3.2:90120960-90120979 | MS.gene42274:CDS | 30.0% | |
| ACGAAATGTATCACATTTGT+CGG | + | chr3.2:90120960-90120979 | None:intergenic | 30.0% | |
| CAAATGTGATACATTTCGTA+GGG | - | chr3.2:90120961-90120980 | MS.gene42274:CDS | 30.0% | |
| GCATAAAATAGTATCCATAC+CGG | + | chr3.2:90120848-90120867 | None:intergenic | 30.0% | |
| TATGTCTTTCCGAACATTAA+GGG | - | chr3.2:90121070-90121089 | MS.gene42274:CDS | 30.0% | |
| ! | ATTGTTGATCTCATCGATTA+TGG | - | chr3.2:90120884-90120903 | MS.gene42274:CDS | 30.0% |
| !!! | TTTTTTGTCTGTTGAGATTC+CGG | - | chr3.2:90120826-90120845 | MS.gene42274:CDS | 30.0% |
| AGGGATTAAAAAGAAAGGCA+CGG | - | chr3.2:90121089-90121108 | MS.gene42274:CDS | 35.0% | |
| GGGATGACTTTCAAAATAAG+AGG | - | chr3.2:90121176-90121195 | MS.gene42274:CDS | 35.0% | |
| GTATGTCTTTCCGAACATTA+AGG | - | chr3.2:90121069-90121088 | MS.gene42274:CDS | 35.0% | |
| TCGGAAAGACATACAAGTTA+AGG | + | chr3.2:90121063-90121082 | None:intergenic | 35.0% | |
| TTATACTGGACGCCAAAAAA+CGG | - | chr3.2:90121147-90121166 | MS.gene42274:CDS | 35.0% | |
| ! | GAATGCTGTTCTTTTCTTAG+AGG | - | chr3.2:90120988-90121007 | MS.gene42274:CDS | 35.0% |
| ACGCCAAAAAACGGATAACA+GGG | - | chr3.2:90121156-90121175 | MS.gene42274:CDS | 40.0% | |
| CACTCGTCAATAGATTCTGA+GGG | + | chr3.2:90121040-90121059 | None:intergenic | 40.0% | |
| TTCTTAGAGGAACGTGTCAA+GGG | - | chr3.2:90121001-90121020 | MS.gene42274:CDS | 40.0% | |
| TTTCTTAGAGGAACGTGTCA+AGG | - | chr3.2:90121000-90121019 | MS.gene42274:CDS | 40.0% | |
| ! | GATCTCATCGATTATGGAGA+GGG | - | chr3.2:90120890-90120909 | MS.gene42274:CDS | 40.0% |
| ! | TGATCTCATCGATTATGGAG+AGG | - | chr3.2:90120889-90120908 | MS.gene42274:CDS | 40.0% |
| !! | AATAAGAGGCTTGAGTTGAC+TGG | - | chr3.2:90121190-90121209 | MS.gene42274:CDS | 40.0% |
| !! | GTTCTCTTACTGGCTTATAC+TGG | - | chr3.2:90121133-90121152 | MS.gene42274:CDS | 40.0% |
| !!! | CATCCCTGTTATCCGTTTTT+TGG | + | chr3.2:90121162-90121181 | None:intergenic | 40.0% |
| AAGTGCACTTTGCACATGCT+CGG | - | chr3.2:90121236-90121255 | MS.gene42274:CDS | 45.0% | |
| ATCGATTATGGAGAGGGAGA+TGG | - | chr3.2:90120896-90120915 | MS.gene42274:CDS | 45.0% | |
| CATGGTGAAGGTTCTCTTAC+TGG | - | chr3.2:90121123-90121142 | MS.gene42274:CDS | 45.0% | |
| GACGCCAAAAAACGGATAAC+AGG | - | chr3.2:90121155-90121174 | MS.gene42274:CDS | 45.0% | |
| GCACTCGTCAATAGATTCTG+AGG | + | chr3.2:90121041-90121060 | None:intergenic | 45.0% | |
| TGTCTGTTGAGATTCCGGTA+TGG | - | chr3.2:90120831-90120850 | MS.gene42274:CDS | 45.0% | |
| ! | AAAAAGAAAGGCACGGTACC+TGG | - | chr3.2:90121096-90121115 | MS.gene42274:CDS | 45.0% |
| ! | TCGTCAATAGATTCTGAGGG+CGG | + | chr3.2:90121037-90121056 | None:intergenic | 45.0% |
| !! | ACATTTGTCGGCTTCACGAA+TGG | + | chr3.2:90120948-90120967 | None:intergenic | 45.0% |
| GAACCTTCACCATGTATGCC+AGG | + | chr3.2:90121117-90121136 | None:intergenic | 50.0% | |
| GCTCTGCAAAGAGACCTCTA+TGG | - | chr3.2:90121274-90121293 | MS.gene42274:CDS | 50.0% | |
| GTACCTGGCATACATGGTGA+AGG | - | chr3.2:90121111-90121130 | MS.gene42274:CDS | 50.0% | |
| CACATGCTCGGAAGCGAATG+GGG | - | chr3.2:90121248-90121267 | MS.gene42274:CDS | 55.0% | |
| GCACATGCTCGGAAGCGAAT+GGG | - | chr3.2:90121247-90121266 | MS.gene42274:CDS | 55.0% | |
| TGCACATGCTCGGAAGCGAA+TGG | - | chr3.2:90121246-90121265 | MS.gene42274:CDS | 55.0% | |
| GGCACGGTACCTGGCATACA+TGG | - | chr3.2:90121105-90121124 | MS.gene42274:CDS | 60.0% | |
| TGCTCGGAAGCGAATGGGGA+AGG | - | chr3.2:90121252-90121271 | MS.gene42274:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 90120803 | 90121303 | 90120803 | ID=MS.gene42274 |
| chr3.2 | mRNA | 90120803 | 90121303 | 90120803 | ID=MS.gene42274.t1;Parent=MS.gene42274 |
| chr3.2 | exon | 90120803 | 90121303 | 90120803 | ID=MS.gene42274.t1.exon1;Parent=MS.gene42274.t1 |
| chr3.2 | CDS | 90120803 | 90121303 | 90120803 | ID=cds.MS.gene42274.t1;Parent=MS.gene42274.t1 |
| Gene Sequence |
| Protein sequence |
