Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42417.t1 | XP_003623880.1 | 100 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 3.20E-28 | 134 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42417.t1 | G7L2K4 | 100.0 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 2.3e-28 | 134.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051328 | MS.gene42417 | 0.81536 | 9.62E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42417.t1 | MTR_7g076630 | 100.000 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 5.09e-41 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42417.t1 | AT4G00585 | 83.051 | 59 | 9 | 1 | 4 | 62 | 2 | 59 | 1.71e-29 | 100 |
Find 23 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTGCGTTTCCAGTGTTT+AGG | 0.292140 | 7.2:+87377700 | None:intergenic |
| GAGCCAATGGAGGAGATTTC+AGG | 0.296342 | 7.2:-87377762 | MS.gene42417:CDS |
| TGTTGCGTTTCCAGTGTTTA+GGG | 0.348623 | 7.2:+87377701 | None:intergenic |
| GAGATTTCAGGTACAAGGTT+TGG | 0.375661 | 7.2:-87377750 | MS.gene42417:CDS |
| TTTAGCTGAGAGTTTGAAGA+TGG | 0.461581 | 7.2:+87377639 | None:intergenic |
| CACGGTAATGGCGGAGCCAA+TGG | 0.484941 | 7.2:-87377775 | MS.gene42417:CDS |
| TAGCTGAGAGTTTGAAGATG+GGG | 0.490985 | 7.2:+87377641 | None:intergenic |
| GTACCTGAAATCTCCTCCAT+TGG | 0.492681 | 7.2:+87377759 | None:intergenic |
| AACACTGCCATCGCTATGTT+CGG | 0.493133 | 7.2:-87377682 | MS.gene42417:CDS |
| TGGAGGAGATTTCAGGTACA+AGG | 0.512900 | 7.2:-87377755 | MS.gene42417:CDS |
| TTAGCTGAGAGTTTGAAGAT+GGG | 0.520876 | 7.2:+87377640 | None:intergenic |
| GGTGGAGCAGATCACGGTAA+TGG | 0.524664 | 7.2:-87377787 | MS.gene42417:CDS |
| TACAAGGTTTGGAGTATGAC+TGG | 0.533279 | 7.2:-87377739 | MS.gene42417:CDS |
| CTCACTGTCGCCCTAAACAC+TGG | 0.552111 | 7.2:-87377711 | MS.gene42417:CDS |
| CAAACTCTCAGCTAAACTTG+AGG | 0.608144 | 7.2:-87377632 | MS.gene42417:intron |
| TGGTATCCAATCCCATGCAT+CGG | 0.619221 | 7.2:+87377165 | None:intergenic |
| GGAGGTGGTGGAGCAGATCA+CGG | 0.622572 | 7.2:-87377793 | MS.gene42417:CDS |
| GGTAATGGCGGAGCCAATGG+AGG | 0.641548 | 7.2:-87377772 | MS.gene42417:CDS |
| GACGATACCGAACATAGCGA+TGG | 0.647243 | 7.2:+87377675 | None:intergenic |
| GGAGCAGATCACGGTAATGG+CGG | 0.651187 | 7.2:-87377784 | MS.gene42417:CDS |
| CAGTGTTTAGGGCGACAGTG+AGG | 0.708119 | 7.2:+87377712 | None:intergenic |
| AAGGTTTGGAGTATGACTGG+TGG | 0.712549 | 7.2:-87377736 | MS.gene42417:CDS |
| TGAAGATGGGGATACAAACG+AGG | 0.746465 | 7.2:+87377653 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTGTTGTTATTATTATT+TGG | - | chr7.2:87377552-87377571 | MS.gene42417:intron | 10.0% |
| !!! | TTTATTGATGATTTTGTGTT+TGG | - | chr7.2:87377701-87377720 | MS.gene42417:CDS | 20.0% |
| ! | AAAATCAAAACATGGAAATC+TGG | + | chr7.2:87377394-87377413 | None:intergenic | 25.0% |
| ! | CAATGTTTACTTTGATACAT+TGG | - | chr7.2:87377646-87377665 | MS.gene42417:CDS | 25.0% |
| ! | GGAGATATAAAATCAAAACA+TGG | + | chr7.2:87377402-87377421 | None:intergenic | 25.0% |
| !! | GGCAAAATTAAGAATGTTTT+GGG | - | chr7.2:87377573-87377592 | MS.gene42417:intron | 25.0% |
| !! | TAACGGAATCTTAATTTGAT+TGG | - | chr7.2:87377523-87377542 | MS.gene42417:intron | 25.0% |
| !! | TGGCAAAATTAAGAATGTTT+TGG | - | chr7.2:87377572-87377591 | MS.gene42417:intron | 25.0% |
| !!! | TAGCGACAATTTTAATCAAA+GGG | - | chr7.2:87377755-87377774 | MS.gene42417:CDS | 25.0% |
| ATAAATGAGGGACTTGATAA+CGG | - | chr7.2:87377506-87377525 | MS.gene42417:intron | 30.0% | |
| ! | GATTTTGTGTTTGGAAAGTT+TGG | - | chr7.2:87377710-87377729 | MS.gene42417:CDS | 30.0% |
| ! | TGATACATTGGTTAATATGC+TGG | - | chr7.2:87377658-87377677 | MS.gene42417:CDS | 30.0% |
| ! | TTTTCGTTGCGAATAAATGA+GGG | - | chr7.2:87377494-87377513 | MS.gene42417:intron | 30.0% |
| !! | AGCGACAATTTTAATCAAAG+GGG | - | chr7.2:87377756-87377775 | MS.gene42417:CDS | 30.0% |
| !! | GTAGCGACAATTTTAATCAA+AGG | - | chr7.2:87377754-87377773 | MS.gene42417:CDS | 30.0% |
| GGAAATCTGGTAAAGAAAAC+AGG | + | chr7.2:87377381-87377400 | None:intergenic | 35.0% | |
| GTTAATGTTAACGACGATCT+GGG | - | chr7.2:87377607-87377626 | MS.gene42417:intron | 35.0% | |
| TGTTAATGTTAACGACGATC+TGG | - | chr7.2:87377606-87377625 | MS.gene42417:intron | 35.0% | |
| TTAGCTGAGAGTTTGAAGAT+GGG | + | chr7.2:87377321-87377340 | None:intergenic | 35.0% | |
| TTTAGCTGAGAGTTTGAAGA+TGG | + | chr7.2:87377322-87377341 | None:intergenic | 35.0% | |
| ! | GTTTTCGTTGCGAATAAATG+AGG | - | chr7.2:87377493-87377512 | MS.gene42417:intron | 35.0% |
| CAAACTCTCAGCTAAACTTG+AGG | - | chr7.2:87377326-87377345 | MS.gene42417:intron | 40.0% | |
| TAGCTGAGAGTTTGAAGATG+GGG | + | chr7.2:87377320-87377339 | None:intergenic | 40.0% | |
| TGTTGCGTTTCCAGTGTTTA+GGG | + | chr7.2:87377260-87377279 | None:intergenic | 40.0% | |
| ! | GAGATTTCAGGTACAAGGTT+TGG | - | chr7.2:87377208-87377227 | MS.gene42417:CDS | 40.0% |
| ! | TACAAGGTTTGGAGTATGAC+TGG | - | chr7.2:87377219-87377238 | MS.gene42417:intron | 40.0% |
| ! | TGAGATTTTCACCGATGCAT+GGG | - | chr7.2:87377782-87377801 | MS.gene42417:CDS | 40.0% |
| AACACTGCCATCGCTATGTT+CGG | - | chr7.2:87377276-87377295 | MS.gene42417:intron | 45.0% | |
| GTACCTGAAATCTCCTCCAT+TGG | + | chr7.2:87377202-87377221 | None:intergenic | 45.0% | |
| GTGTTGCGTTTCCAGTGTTT+AGG | + | chr7.2:87377261-87377280 | None:intergenic | 45.0% | |
| TGAAGATGGGGATACAAACG+AGG | + | chr7.2:87377308-87377327 | None:intergenic | 45.0% | |
| TGGTATCCAATCCCATGCAT+CGG | + | chr7.2:87377796-87377815 | None:intergenic | 45.0% | |
| ! | AAGGTTTGGAGTATGACTGG+TGG | - | chr7.2:87377222-87377241 | MS.gene42417:intron | 45.0% |
| ! | GTGAGATTTTCACCGATGCA+TGG | - | chr7.2:87377781-87377800 | MS.gene42417:CDS | 45.0% |
| ! | TGGAGGAGATTTCAGGTACA+AGG | - | chr7.2:87377203-87377222 | MS.gene42417:CDS | 45.0% |
| ! | TTTTCACCGATGCATGGGAT+TGG | - | chr7.2:87377787-87377806 | MS.gene42417:CDS | 45.0% |
| !!! | AAAAAAAATTGATAATAATT+TGG | + | chr7.2:87377423-87377442 | None:intergenic | 5.0% |
| !!! | AATTATTATCAATTTTTTTT+AGG | - | chr7.2:87377423-87377442 | MS.gene42417:intron | 5.0% |
| !!! | ATTATTATCAATTTTTTTTA+GGG | - | chr7.2:87377424-87377443 | MS.gene42417:intron | 5.0% |
| GACGATACCGAACATAGCGA+TGG | + | chr7.2:87377286-87377305 | None:intergenic | 50.0% | |
| GAGCCAATGGAGGAGATTTC+AGG | - | chr7.2:87377196-87377215 | MS.gene42417:CDS | 50.0% | |
| CTCACTGTCGCCCTAAACAC+TGG | - | chr7.2:87377247-87377266 | MS.gene42417:intron | 55.0% | |
| GGTGGAGCAGATCACGGTAA+TGG | - | chr7.2:87377171-87377190 | MS.gene42417:CDS | 55.0% | |
| ! | CAGTGTTTAGGGCGACAGTG+AGG | + | chr7.2:87377249-87377268 | None:intergenic | 55.0% |
| ! | GGAGCAGATCACGGTAATGG+CGG | - | chr7.2:87377174-87377193 | MS.gene42417:CDS | 55.0% |
| ! | CACGGTAATGGCGGAGCCAA+TGG | - | chr7.2:87377183-87377202 | MS.gene42417:CDS | 60.0% |
| ! | GGTAATGGCGGAGCCAATGG+AGG | - | chr7.2:87377186-87377205 | MS.gene42417:CDS | 60.0% |
| !! | GGAGGTGGTGGAGCAGATCA+CGG | - | chr7.2:87377165-87377184 | MS.gene42417:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 87377162 | 87377818 | 87377162 | ID=MS.gene42417 |
| chr7.2 | mRNA | 87377162 | 87377818 | 87377162 | ID=MS.gene42417.t1;Parent=MS.gene42417 |
| chr7.2 | exon | 87377633 | 87377818 | 87377633 | ID=MS.gene42417.t1.exon1;Parent=MS.gene42417.t1 |
| chr7.2 | CDS | 87377633 | 87377818 | 87377633 | ID=cds.MS.gene42417.t1;Parent=MS.gene42417.t1 |
| chr7.2 | exon | 87377162 | 87377233 | 87377162 | ID=MS.gene42417.t1.exon2;Parent=MS.gene42417.t1 |
| chr7.2 | CDS | 87377162 | 87377233 | 87377162 | ID=cds.MS.gene42417.t1;Parent=MS.gene42417.t1 |
| Gene Sequence |
| Protein sequence |
