Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42420.t1 | XP_003623879.1 | 93.1 | 160 | 10 | 1 | 1 | 160 | 1 | 159 | 1.70E-75 | 292 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42420.t1 | Q9T0A4 | 45.9 | 135 | 63 | 4 | 6 | 135 | 7 | 136 | 6.5e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42420.t1 | G7L2K3 | 93.1 | 160 | 10 | 1 | 1 | 160 | 1 | 159 | 1.2e-75 | 292.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056719 | MS.gene42420 | 0.805003 | 1.65E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42420.t1 | MTR_7g076620 | 93.125 | 160 | 10 | 1 | 1 | 160 | 1 | 159 | 1.22e-106 | 301 |
| MS.gene42420.t1 | MTR_8g032260 | 49.296 | 142 | 66 | 3 | 1 | 136 | 1 | 142 | 1.21e-41 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42420.t1 | AT4G23890 | 45.926 | 135 | 63 | 4 | 6 | 135 | 7 | 136 | 1.65e-30 | 111 |
Find 28 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTTTCCTGGTGGTGAAAA+AGG | 0.216558 | 7.2:+87383331 | MS.gene42420:CDS |
| TTTCTTTCTTCAATAGTAAA+AGG | 0.267304 | 7.2:-87383400 | None:intergenic |
| GGACTATGCAATGGAGAAAA+AGG | 0.281632 | 7.2:+87383151 | MS.gene42420:CDS |
| TCCTTCTCAAAACCATCTTC+TGG | 0.297002 | 7.2:-87383289 | None:intergenic |
| GGGCTAACTGGAGGGTTTCC+TGG | 0.314218 | 7.2:+87383319 | MS.gene42420:CDS |
| GCTACTCTTCAAGGCCTTCA+TGG | 0.325932 | 7.2:+87382986 | MS.gene42420:CDS |
| AAGGAAATGATGGGGCTAAC+TGG | 0.367060 | 7.2:+87383307 | MS.gene42420:CDS |
| AGAGTTGAACTATGTTGCTT+TGG | 0.385186 | 7.2:-87383443 | None:intergenic |
| GATGATCTTGGCCTATGAAC+TGG | 0.387695 | 7.2:-87383024 | None:intergenic |
| GACTTGAACAAGAGCTGAAA+AGG | 0.446004 | 7.2:+87383173 | MS.gene42420:CDS |
| CGTGGTTGTAGTTGAGAAGC+TGG | 0.452047 | 7.2:-87383091 | None:intergenic |
| ATTGCTGCAATTCCAGAAGA+TGG | 0.464592 | 7.2:+87383277 | MS.gene42420:CDS |
| CTCCTACGATCCCAGTTCAT+AGG | 0.487970 | 7.2:+87383013 | MS.gene42420:CDS |
| AAAGGTTGAGAAGAATTCCT+AGG | 0.555335 | 7.2:-87383382 | None:intergenic |
| ATGGATCAGAAAAGTTGACT+AGG | 0.563942 | 7.2:+87383251 | MS.gene42420:CDS |
| ATCGTAGGAGAGAGCCATGA+AGG | 0.575050 | 7.2:-87383000 | None:intergenic |
| GAGGGAAGTGATGATGATCT+TGG | 0.577411 | 7.2:-87383036 | None:intergenic |
| ATGATCTTGGCCTATGAACT+GGG | 0.582701 | 7.2:-87383023 | None:intergenic |
| AAAGTGCAAGAAAACTCAGA+TGG | 0.584856 | 7.2:+87383232 | MS.gene42420:CDS |
| AAATGATGGGGCTAACTGGA+GGG | 0.586145 | 7.2:+87383311 | MS.gene42420:CDS |
| GGCCTATGAACTGGGATCGT+AGG | 0.587300 | 7.2:-87383015 | None:intergenic |
| GAAATGATGGGGCTAACTGG+AGG | 0.588055 | 7.2:+87383310 | MS.gene42420:CDS |
| TGGATCAGAAAAGTTGACTA+GGG | 0.600219 | 7.2:+87383252 | MS.gene42420:CDS |
| GGAGGAAGAGGACTATGCAA+TGG | 0.605614 | 7.2:+87383142 | MS.gene42420:CDS |
| CTAACTGGAGGGTTTCCTGG+TGG | 0.624925 | 7.2:+87383322 | MS.gene42420:CDS |
| TTGCTTTGGATTCTTCAACA+AGG | 0.649439 | 7.2:-87383429 | None:intergenic |
| GGTTGAGAAGAATTCCTAGG+TGG | 0.675895 | 7.2:-87383379 | None:intergenic |
| AGTATGTTGAATTCAGCACG+TGG | 0.711591 | 7.2:-87383109 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAACATACTAAAAATGA+TGG | + | chr7.2:87383120-87383139 | MS.gene42420:CDS | 20.0% |
| !! | TTCAACATACTAAAAATGAT+GGG | + | chr7.2:87383121-87383140 | MS.gene42420:CDS | 20.0% |
| !! | TTTCTTTCTTCAATAGTAAA+AGG | - | chr7.2:87383403-87383422 | None:intergenic | 20.0% |
| ! | AACATACTAAAAATGATGGG+AGG | + | chr7.2:87383124-87383143 | MS.gene42420:CDS | 30.0% |
| !!! | GTTTTCTTGCACTTTTTCAT+TGG | - | chr7.2:87383227-87383246 | None:intergenic | 30.0% |
| AAAGGTTGAGAAGAATTCCT+AGG | - | chr7.2:87383385-87383404 | None:intergenic | 35.0% | |
| AAAGTGCAAGAAAACTCAGA+TGG | + | chr7.2:87383232-87383251 | MS.gene42420:CDS | 35.0% | |
| ATGGATCAGAAAAGTTGACT+AGG | + | chr7.2:87383251-87383270 | MS.gene42420:CDS | 35.0% | |
| TCATTCAGAAAAATCCACCT+AGG | + | chr7.2:87383365-87383384 | MS.gene42420:CDS | 35.0% | |
| TGGATCAGAAAAGTTGACTA+GGG | + | chr7.2:87383252-87383271 | MS.gene42420:CDS | 35.0% | |
| ! | TTGCTTTGGATTCTTCAACA+AGG | - | chr7.2:87383432-87383451 | None:intergenic | 35.0% |
| ! | TTGTGGAAGTTTTTGTGAGA+GGG | - | chr7.2:87383057-87383076 | None:intergenic | 35.0% |
| !!! | GGTTTTGAGAAGGAAATGAT+GGG | + | chr7.2:87383298-87383317 | MS.gene42420:CDS | 35.0% |
| !!! | GTTTTGAGAAGGAAATGATG+GGG | + | chr7.2:87383299-87383318 | MS.gene42420:CDS | 35.0% |
| !!! | TGGTTTTGAGAAGGAAATGA+TGG | + | chr7.2:87383297-87383316 | MS.gene42420:CDS | 35.0% |
| AGTATGTTGAATTCAGCACG+TGG | - | chr7.2:87383112-87383131 | None:intergenic | 40.0% | |
| ATGATCTTGGCCTATGAACT+GGG | - | chr7.2:87383026-87383045 | None:intergenic | 40.0% | |
| ATTGCTGCAATTCCAGAAGA+TGG | + | chr7.2:87383277-87383296 | MS.gene42420:CDS | 40.0% | |
| GACTTGAACAAGAGCTGAAA+AGG | + | chr7.2:87383173-87383192 | MS.gene42420:CDS | 40.0% | |
| GGACTATGCAATGGAGAAAA+AGG | + | chr7.2:87383151-87383170 | MS.gene42420:CDS | 40.0% | |
| TCCTTCTCAAAACCATCTTC+TGG | - | chr7.2:87383292-87383311 | None:intergenic | 40.0% | |
| ! | CTAAAAATGATGGGAGGAAG+AGG | + | chr7.2:87383130-87383149 | MS.gene42420:CDS | 40.0% |
| ! | GTTGTGGAAGTTTTTGTGAG+AGG | - | chr7.2:87383058-87383077 | None:intergenic | 40.0% |
| ! | TGGTTTTCTGTGATGTGTTG+TGG | - | chr7.2:87383074-87383093 | None:intergenic | 40.0% |
| !!! | GTTGCTTTTGCTACTCTTCA+AGG | + | chr7.2:87382977-87382996 | MS.gene42420:CDS | 40.0% |
| !!! | TCCAGAAGATGGTTTTGAGA+AGG | + | chr7.2:87383288-87383307 | MS.gene42420:CDS | 40.0% |
| GATGATCTTGGCCTATGAAC+TGG | - | chr7.2:87383027-87383046 | None:intergenic | 45.0% | |
| GGTTGAGAAGAATTCCTAGG+TGG | - | chr7.2:87383382-87383401 | None:intergenic | 45.0% | |
| ! | AAATGATGGGGCTAACTGGA+GGG | + | chr7.2:87383311-87383330 | MS.gene42420:CDS | 45.0% |
| ! | AAGGAAATGATGGGGCTAAC+TGG | + | chr7.2:87383307-87383326 | MS.gene42420:CDS | 45.0% |
| ! | GAGGGAAGTGATGATGATCT+TGG | - | chr7.2:87383039-87383058 | None:intergenic | 45.0% |
| ! | GGTTTCCTGGTGGTGAAAAA+GGG | + | chr7.2:87383332-87383351 | MS.gene42420:CDS | 45.0% |
| !! | ATCAACCCTTTTTCACCACC+AGG | - | chr7.2:87383340-87383359 | None:intergenic | 45.0% |
| ATCGTAGGAGAGAGCCATGA+AGG | - | chr7.2:87383003-87383022 | None:intergenic | 50.0% | |
| CGTGGTTGTAGTTGAGAAGC+TGG | - | chr7.2:87383094-87383113 | None:intergenic | 50.0% | |
| CTCCTACGATCCCAGTTCAT+AGG | + | chr7.2:87383013-87383032 | MS.gene42420:CDS | 50.0% | |
| GCTACTCTTCAAGGCCTTCA+TGG | + | chr7.2:87382986-87383005 | MS.gene42420:CDS | 50.0% | |
| GGAGGAAGAGGACTATGCAA+TGG | + | chr7.2:87383142-87383161 | MS.gene42420:CDS | 50.0% | |
| ! | GAAATGATGGGGCTAACTGG+AGG | + | chr7.2:87383310-87383329 | MS.gene42420:CDS | 50.0% |
| ! | GGGTTTCCTGGTGGTGAAAA+AGG | + | chr7.2:87383331-87383350 | MS.gene42420:CDS | 50.0% |
| CTAACTGGAGGGTTTCCTGG+TGG | + | chr7.2:87383322-87383341 | MS.gene42420:CDS | 55.0% | |
| GGCCTATGAACTGGGATCGT+AGG | - | chr7.2:87383018-87383037 | None:intergenic | 55.0% | |
| GGGCTAACTGGAGGGTTTCC+TGG | + | chr7.2:87383319-87383338 | MS.gene42420:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 87382974 | 87383456 | 87382974 | ID=MS.gene42420 |
| chr7.2 | mRNA | 87382974 | 87383456 | 87382974 | ID=MS.gene42420.t1;Parent=MS.gene42420 |
| chr7.2 | exon | 87382974 | 87383456 | 87382974 | ID=MS.gene42420.t1.exon1;Parent=MS.gene42420.t1 |
| chr7.2 | CDS | 87382974 | 87383456 | 87382974 | ID=cds.MS.gene42420.t1;Parent=MS.gene42420.t1 |
| Gene Sequence |
| Protein sequence |
