Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42487.t1 | KEH28568.1 | 78.9 | 57 | 12 | 0 | 39 | 95 | 294 | 350 | 2.20E-15 | 91.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42487.t1 | A0A072UHG8 | 78.9 | 57 | 12 | 0 | 39 | 95 | 294 | 350 | 1.6e-15 | 91.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene42487.t1 | TR | HMG |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05164 | MS.gene42487 | 0.908188 | 2.25E-81 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene42487.t1 | MTR_6g048860 | 82.692 | 52 | 9 | 0 | 39 | 90 | 266 | 317 | 3.42e-24 | 94.0 |
| MS.gene42487.t1 | MTR_3g037670 | 80.952 | 42 | 8 | 0 | 39 | 80 | 224 | 265 | 8.42e-18 | 76.3 |
| MS.gene42487.t1 | MTR_4g007610 | 78.947 | 57 | 12 | 0 | 39 | 95 | 294 | 350 | 1.07e-17 | 76.6 |
| MS.gene42487.t1 | MTR_8g107350 | 76.190 | 42 | 10 | 0 | 39 | 80 | 192 | 233 | 1.10e-16 | 73.2 |
| MS.gene42487.t1 | MTR_5g038770 | 50.847 | 59 | 28 | 1 | 11 | 68 | 6 | 64 | 9.00e-12 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGGATAAACAACATTATT+TGG | 0.112137 | 6.1:+67964218 | MS.gene42487:intron |
| AAATTATGATGTTAATAAAA+TGG | 0.232813 | 6.1:+67964080 | MS.gene42487:CDS |
| TCTATCTGCAACTGTGAAAT+TGG | 0.248781 | 6.1:-67963646 | None:intergenic |
| TTATATTTCAGGGATAAGTT+TGG | 0.382899 | 6.1:+67964033 | MS.gene42487:intron |
| GTGTTACCTGTGAGCGAAAG+AGG | 0.447507 | 6.1:-67963683 | None:intergenic |
| GTGAAGCATTCCACCATTCT+CGG | 0.464051 | 6.1:-67964398 | None:intergenic |
| AGGATGAAGTTCAATAGATC+CGG | 0.465533 | 6.1:-67963623 | None:intergenic |
| AACATGAGAAACTTAAGAAA+AGG | 0.495000 | 6.1:+67964265 | MS.gene42487:CDS |
| AGGACTTACTTCATTACTCA+TGG | 0.496503 | 6.1:-67964121 | None:intergenic |
| ATAAAATGGTTGTTAAAGCA+TGG | 0.505549 | 6.1:+67964094 | MS.gene42487:CDS |
| CCGGGACTGTGAAGCTCGAT+CGG | 0.546676 | 6.1:-67963604 | None:intergenic |
| GAATAGGGTGACTCCGAGAA+TGG | 0.550110 | 6.1:+67964385 | MS.gene42487:intron |
| CCGATCGAGCTTCACAGTCC+CGG | 0.552827 | 6.1:+67963604 | MS.gene42487:CDS |
| CGGTGAAGTGTGAAGCTCCA+TGG | 0.558822 | 6.1:-67963584 | None:intergenic |
| ATCTGCAACTGTGAAATTGG+AGG | 0.566506 | 6.1:-67963643 | None:intergenic |
| GGATGAAGTTCAATAGATCC+GGG | 0.594180 | 6.1:-67963622 | None:intergenic |
| GGATAAAGCATCTAAGCGCA+AGG | 0.626619 | 6.1:+67964239 | MS.gene42487:CDS |
| TAGGGTGACTCCGAGAATGG+TGG | 0.628698 | 6.1:+67964388 | MS.gene42487:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATGATGTTAATAAAA+TGG | + | chr6.1:67964080-67964099 | MS.gene42487:CDS | 10.0% |
| !! | AAGTATAAAATTTATATTTC+AGG | + | chr6.1:67964022-67964041 | MS.gene42487:intron | 10.0% |
| !! | AGTATAAAATTTATATTTCA+GGG | + | chr6.1:67964023-67964042 | MS.gene42487:intron | 10.0% |
| !! | ATTGATTATATTATTTAATC+AGG | + | chr6.1:67964200-67964219 | MS.gene42487:intron | 10.0% |
| !! | AACACAAAATCAATTAAATT+TGG | - | chr6.1:67964304-67964323 | None:intergenic | 15.0% |
| !! | ACACAAAATCAATTAAATTT+GGG | - | chr6.1:67964303-67964322 | None:intergenic | 15.0% |
| !! | AAAGAAGATTTCTTATTCTT+TGG | - | chr6.1:67963965-67963984 | None:intergenic | 20.0% |
| ! | AACATGAGAAACTTAAGAAA+AGG | + | chr6.1:67964265-67964284 | MS.gene42487:CDS | 25.0% |
| ! | ATAAAATGGTTGTTAAAGCA+TGG | + | chr6.1:67964094-67964113 | MS.gene42487:CDS | 25.0% |
| ! | TCAGGATAAACAACATTATT+TGG | + | chr6.1:67964218-67964237 | MS.gene42487:intron | 25.0% |
| ! | TTATATTTCAGGGATAAGTT+TGG | + | chr6.1:67964033-67964052 | MS.gene42487:intron | 25.0% |
| ! | TTTGTGTGTTTGATTGAATA+GGG | + | chr6.1:67964370-67964389 | MS.gene42487:intron | 25.0% |
| CATGTGTTGTAATATACTAG+AGG | - | chr6.1:67964144-67964163 | None:intergenic | 30.0% | |
| CTTTGTGTGTTTGATTGAAT+AGG | + | chr6.1:67964369-67964388 | MS.gene42487:intron | 30.0% | |
| !! | TTTCTTTGAAATCCATGTGT+TGG | + | chr6.1:67963933-67963952 | MS.gene42487:intron | 30.0% |
| AGGACTTACTTCATTACTCA+TGG | - | chr6.1:67964124-67964143 | None:intergenic | 35.0% | |
| AGGATGAAGTTCAATAGATC+CGG | - | chr6.1:67963626-67963645 | None:intergenic | 35.0% | |
| TCTATCTGCAACTGTGAAAT+TGG | - | chr6.1:67963649-67963668 | None:intergenic | 35.0% | |
| ATCTGCAACTGTGAAATTGG+AGG | - | chr6.1:67963646-67963665 | None:intergenic | 40.0% | |
| GGATGAAGTTCAATAGATCC+GGG | - | chr6.1:67963625-67963644 | None:intergenic | 40.0% | |
| !!! | TTTTTTCCTCTTTCGCTCAC+AGG | + | chr6.1:67963677-67963696 | MS.gene42487:CDS | 40.0% |
| CTTTGGCAACATCCAACACA+TGG | - | chr6.1:67963948-67963967 | None:intergenic | 45.0% | |
| ! | GGATAAAGCATCTAAGCGCA+AGG | + | chr6.1:67964239-67964258 | MS.gene42487:CDS | 45.0% |
| ! | GTGAAGCATTCCACCATTCT+CGG | - | chr6.1:67964401-67964420 | None:intergenic | 45.0% |
| GAATAGGGTGACTCCGAGAA+TGG | + | chr6.1:67964385-67964404 | MS.gene42487:intron | 50.0% | |
| GTGTTACCTGTGAGCGAAAG+AGG | - | chr6.1:67963686-67963705 | None:intergenic | 50.0% | |
| TAGGGTGACTCCGAGAATGG+TGG | + | chr6.1:67964388-67964407 | MS.gene42487:intron | 55.0% | |
| CCGATCGAGCTTCACAGTCC+CGG | + | chr6.1:67963604-67963623 | MS.gene42487:CDS | 60.0% | |
| CCGGGACTGTGAAGCTCGAT+CGG | - | chr6.1:67963607-67963626 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 67963586 | 67964427 | 67963586 | ID=MS.gene42487 |
| chr6.1 | mRNA | 67963586 | 67964427 | 67963586 | ID=MS.gene42487.t1;Parent=MS.gene42487 |
| chr6.1 | exon | 67963586 | 67963698 | 67963586 | ID=MS.gene42487.t1.exon1;Parent=MS.gene42487.t1 |
| chr6.1 | CDS | 67963586 | 67963698 | 67963586 | ID=cds.MS.gene42487.t1;Parent=MS.gene42487.t1 |
| chr6.1 | exon | 67964044 | 67964134 | 67964044 | ID=MS.gene42487.t1.exon2;Parent=MS.gene42487.t1 |
| chr6.1 | CDS | 67964044 | 67964134 | 67964044 | ID=cds.MS.gene42487.t1;Parent=MS.gene42487.t1 |
| chr6.1 | exon | 67964222 | 67964286 | 67964222 | ID=MS.gene42487.t1.exon3;Parent=MS.gene42487.t1 |
| chr6.1 | CDS | 67964222 | 67964286 | 67964222 | ID=cds.MS.gene42487.t1;Parent=MS.gene42487.t1 |
| chr6.1 | exon | 67964391 | 67964427 | 67964391 | ID=MS.gene42487.t1.exon4;Parent=MS.gene42487.t1 |
| chr6.1 | CDS | 67964391 | 67964427 | 67964391 | ID=cds.MS.gene42487.t1;Parent=MS.gene42487.t1 |
| Gene Sequence |
| Protein sequence |
