AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43036

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43036.t1 MTR_2g086530 98.711 388 5 0 1 388 1 388 0.0 773
MS.gene43036.t1 MTR_2g086530 99.183 367 3 0 22 388 1 367 0.0 736
MS.gene43036.t1 MTR_4g052970 66.935 372 115 2 16 385 16 381 0.0 514
MS.gene43036.t1 MTR_1g077990 61.950 318 118 2 30 345 32 348 7.32e-144 414
MS.gene43036.t1 MTR_4g084480 50.000 338 167 1 10 345 13 350 2.82e-124 364
MS.gene43036.t1 MTR_4g084480 50.000 338 167 1 10 345 6 343 3.35e-124 364
MS.gene43036.t1 MTR_4g084480 50.000 338 167 1 10 345 13 350 3.79e-124 364
MS.gene43036.t1 MTR_2g019345 48.980 343 170 3 5 345 2 341 3.34e-117 346
MS.gene43036.t1 MTR_4g010200 45.070 355 185 4 4 351 7 358 4.91e-115 340
MS.gene43036.t1 MTR_4g114850 44.932 365 187 4 29 383 26 386 4.58e-110 328
MS.gene43036.t1 MTR_4g114850 48.758 322 158 2 29 345 26 345 3.55e-109 325
MS.gene43036.t1 MTR_4g114850 48.758 322 158 2 29 345 26 345 4.12e-109 325
MS.gene43036.t1 MTR_4g069940 42.655 354 199 3 2 352 3 355 1.04e-94 291
MS.gene43036.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 2.85e-94 288
MS.gene43036.t1 MTR_3g080410 43.732 343 181 5 22 353 19 360 8.44e-94 285
MS.gene43036.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 1.62e-93 289
MS.gene43036.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 3.40e-93 288
MS.gene43036.t1 MTR_5g085580 45.092 326 174 3 24 345 20 344 3.64e-93 287
MS.gene43036.t1 MTR_1g022160 43.210 324 180 3 29 348 24 347 4.32e-93 287
MS.gene43036.t1 MTR_3g116510 42.407 349 193 4 6 351 10 353 3.68e-92 284
MS.gene43036.t1 MTR_1g056640 44.237 321 174 4 29 345 38 357 2.21e-91 283
MS.gene43036.t1 MTR_6g088885 41.547 349 197 3 5 350 6 350 5.04e-89 278
MS.gene43036.t1 MTR_1g007810 42.433 337 182 6 10 342 1 329 6.89e-87 268
MS.gene43036.t1 MTR_7g053500 40.719 334 180 7 30 351 27 354 7.56e-85 266
MS.gene43036.t1 MTR_7g026340 40.625 320 180 7 30 345 24 337 6.54e-80 253
MS.gene43036.t1 MTR_4g083500 41.617 334 188 6 28 357 43 373 1.05e-77 249
MS.gene43036.t1 MTR_8g102340 40.752 319 157 4 30 348 29 315 4.71e-77 246
MS.gene43036.t1 MTR_8g012400 36.975 357 214 7 10 356 7 362 5.44e-77 245
MS.gene43036.t1 MTR_4g088155 36.944 360 204 7 11 356 8 358 3.27e-76 243
MS.gene43036.t1 MTR_4g076490 36.288 361 200 6 2 344 54 402 1.60e-75 240
MS.gene43036.t1 MTR_8g085720 35.467 375 229 5 1 366 1 371 2.24e-74 239
MS.gene43036.t1 MTR_4g132280 38.154 325 194 3 23 341 39 362 5.13e-71 231
MS.gene43036.t1 MTR_3g083580 36.308 325 199 4 26 343 23 346 5.28e-70 228
MS.gene43036.t1 MTR_4g076470 37.278 338 188 8 12 341 3 324 2.81e-69 221
MS.gene43036.t1 MTR_4g076430 36.095 338 198 8 10 343 3 326 1.08e-67 217
MS.gene43036.t1 MTR_1g052430 38.679 318 181 8 23 336 28 335 1.30e-67 219
MS.gene43036.t1 MTR_7g053500 37.993 279 163 5 77 351 5 277 1.45e-66 216
MS.gene43036.t1 MTR_4g076440 34.718 337 204 7 10 343 3 326 4.73e-66 213
MS.gene43036.t1 MTR_4g076570 37.304 319 190 6 29 344 22 333 9.22e-66 213
MS.gene43036.t1 MTR_4g076255 36.709 316 185 6 30 343 26 328 3.34e-65 211
MS.gene43036.t1 MTR_2g034480 36.443 343 196 10 30 366 28 354 5.72e-65 211
MS.gene43036.t1 MTR_3g065460 36.628 344 209 5 8 345 5 345 7.88e-65 214
MS.gene43036.t1 MTR_1g031900 35.380 342 214 4 10 345 5 345 3.39e-64 213
MS.gene43036.t1 MTR_1g031900 35.380 342 214 4 10 345 5 345 6.00e-64 212
MS.gene43036.t1 MTR_3g116510 45.205 219 120 0 133 351 1 219 1.18e-62 204
MS.gene43036.t1 MTR_4g076500 36.538 312 186 6 30 338 79 381 3.37e-62 205
MS.gene43036.t1 MTR_8g012400 38.202 267 160 4 94 356 17 282 5.45e-61 201
MS.gene43036.t1 MTR_5g078200 35.000 340 214 3 12 345 6 344 5.77e-61 207
MS.gene43036.t1 MTR_7g081350 33.333 342 212 9 8 343 4 335 1.86e-60 199
MS.gene43036.t1 MTR_2g034470 35.168 327 194 10 30 349 31 346 3.31e-60 199
MS.gene43036.t1 MTR_5g078200 36.218 312 192 3 40 345 1 311 1.27e-58 201
MS.gene43036.t1 MTR_2g034440 35.144 313 187 9 30 336 23 325 1.62e-58 194
MS.gene43036.t1 MTR_2g034470 34.891 321 191 10 36 349 2 311 1.39e-57 191
MS.gene43036.t1 MTR_7g081370 31.778 343 204 10 8 343 4 323 3.46e-53 179
MS.gene43036.t1 MTR_5g081720 34.304 309 180 11 45 342 53 349 4.06e-47 165
MS.gene43036.t1 MTR_4g124440 32.303 356 210 16 7 345 6 347 1.59e-45 163
MS.gene43036.t1 MTR_6g032820 29.781 366 225 13 4 351 3 354 7.30e-45 160
MS.gene43036.t1 MTR_8g092070 30.793 328 198 14 30 341 35 349 7.38e-45 163
MS.gene43036.t1 MTR_7g085390 32.733 333 202 14 30 349 27 350 8.88e-45 161
MS.gene43036.t1 MTR_4g112025 31.285 358 221 12 4 346 6 353 9.55e-45 162
MS.gene43036.t1 MTR_1g010120 31.844 358 212 16 7 349 9 349 2.03e-44 160
MS.gene43036.t1 MTR_2g027560 31.343 335 205 13 30 348 29 354 6.59e-43 156
MS.gene43036.t1 MTR_3g095050 31.498 327 197 12 29 341 34 347 1.81e-39 147
MS.gene43036.t1 MTR_1g053320 29.213 356 228 12 11 353 16 360 2.14e-39 147
MS.gene43036.t1 MTR_3g108600 30.448 335 193 13 30 352 32 338 8.55e-39 145
MS.gene43036.t1 MTR_3g031770 29.042 334 202 13 30 345 28 344 2.30e-30 122
MS.gene43036.t1 MTR_4g134280 32.117 274 162 12 114 372 3 267 6.97e-30 119
MS.gene43036.t1 MTR_4g036680 42.727 110 50 5 243 351 80 177 1.79e-18 84.0
MS.gene43036.t1 MTR_4g036775 42.727 110 50 5 243 351 80 177 1.79e-18 84.0
MS.gene43036.t1 MTR_6g078180 50.000 50 25 0 90 139 5 54 4.91e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43036.t1 AT2G27500 63.277 354 121 3 26 376 26 373 1.01e-164 466
MS.gene43036.t1 AT2G27500 62.327 361 127 3 26 383 26 380 1.05e-164 467
MS.gene43036.t1 AT1G32860 53.868 349 157 2 1 345 1 349 1.20e-134 392
MS.gene43036.t1 AT2G27500 60.390 308 113 3 79 383 1 302 2.38e-134 387
MS.gene43036.t1 AT5G42100 54.969 322 140 3 30 346 28 349 6.40e-125 367
MS.gene43036.t1 AT5G42100 54.969 322 140 3 30 346 28 349 9.89e-125 367
MS.gene43036.t1 AT1G30080 47.875 353 173 3 3 344 1 353 2.18e-115 341
MS.gene43036.t1 AT1G30080 47.875 353 173 3 3 344 1 353 3.10e-115 342
MS.gene43036.t1 AT3G15800 50.311 322 154 3 29 345 43 363 2.56e-114 339
MS.gene43036.t1 AT4G18340 45.349 344 185 2 4 345 10 352 3.58e-111 330
MS.gene43036.t1 AT4G18340 45.349 344 185 2 4 345 10 352 4.14e-111 331
MS.gene43036.t1 AT2G26600 44.751 362 185 4 29 382 34 388 6.02e-109 325
MS.gene43036.t1 AT4G26830 43.804 347 185 6 1 343 1 341 4.85e-97 297
MS.gene43036.t1 AT4G26830 45.283 318 168 4 30 343 30 345 2.41e-95 293
MS.gene43036.t1 AT2G05790 44.345 336 176 6 30 356 26 359 1.13e-93 289
MS.gene43036.t1 AT5G55180 42.901 324 180 3 29 348 27 349 1.24e-93 288
MS.gene43036.t1 AT5G55180 42.901 324 180 3 29 348 27 349 1.61e-93 288
MS.gene43036.t1 AT2G26600 47.260 292 146 2 94 382 8 294 6.05e-93 281
MS.gene43036.t1 AT5G56590 44.099 322 176 3 26 343 23 344 1.06e-92 287
MS.gene43036.t1 AT2G16230 42.197 346 189 4 24 358 19 364 8.18e-92 285
MS.gene43036.t1 AT5G42720 42.566 343 190 5 8 345 6 346 1.67e-91 282
MS.gene43036.t1 AT5G24318 41.159 345 192 6 7 345 7 346 1.09e-90 278
MS.gene43036.t1 AT5G24318 41.159 345 192 6 7 345 7 346 1.84e-89 277
MS.gene43036.t1 AT5G24318 41.159 345 192 6 7 345 7 346 3.08e-89 277
MS.gene43036.t1 AT4G34480 42.286 350 192 5 1 345 1 345 6.77e-88 270
MS.gene43036.t1 AT4G34480 41.713 362 196 6 1 352 1 357 2.62e-86 271
MS.gene43036.t1 AT4G29360 42.210 353 190 6 6 348 10 358 1.04e-83 265
MS.gene43036.t1 AT3G07320 41.265 332 177 6 30 349 26 351 2.13e-83 262
MS.gene43036.t1 AT4G29360 43.168 322 179 3 30 348 28 348 1.19e-82 262
MS.gene43036.t1 AT4G14080 37.067 375 216 6 1 356 1 374 2.11e-78 249
MS.gene43036.t1 AT2G01630 40.248 323 186 3 29 345 23 344 8.30e-78 245
MS.gene43036.t1 AT2G01630 40.248 323 186 3 29 345 23 344 2.20e-77 246
MS.gene43036.t1 AT2G01630 40.373 322 185 3 30 345 24 344 2.20e-76 245
MS.gene43036.t1 AT3G23770 40.120 334 190 6 30 354 38 370 3.46e-74 238
MS.gene43036.t1 AT4G16260 37.092 337 200 6 7 341 4 330 1.05e-73 233
MS.gene43036.t1 AT3G55430 37.912 364 205 12 2 358 7 356 3.60e-72 233
MS.gene43036.t1 AT1G11820 38.507 335 198 4 30 357 45 378 4.42e-72 230
MS.gene43036.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene43036.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene43036.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene43036.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene43036.t1 AT3G13560 37.651 332 192 4 20 341 17 343 7.70e-72 233
MS.gene43036.t1 AT1G11820 39.375 320 187 3 30 343 45 363 9.98e-71 229
MS.gene43036.t1 AT1G11820 39.375 320 187 3 30 343 45 363 1.83e-70 230
MS.gene43036.t1 AT2G39640 40.122 329 183 6 30 356 31 347 2.21e-69 228
MS.gene43036.t1 AT3G46570 39.017 346 196 10 9 345 5 344 6.00e-69 221
MS.gene43036.t1 AT4G16260 37.662 308 182 5 36 341 2 301 6.85e-69 220
MS.gene43036.t1 AT3G57260 35.608 337 200 10 10 343 14 336 2.80e-66 214
MS.gene43036.t1 AT3G57260 35.608 337 200 10 10 343 30 352 5.38e-66 214
MS.gene43036.t1 AT3G57270 35.952 331 196 9 20 343 16 337 5.48e-65 211
MS.gene43036.t1 AT4G34480 44.672 244 130 1 114 352 3 246 2.91e-63 207
MS.gene43036.t1 AT3G61810 34.365 323 194 7 30 343 56 369 2.99e-62 204
MS.gene43036.t1 AT5G24318 45.024 211 111 2 136 345 1 207 1.41e-61 201
MS.gene43036.t1 AT1G66250 35.583 326 202 4 29 348 32 355 4.15e-61 205
MS.gene43036.t1 AT3G57240 33.968 315 191 8 30 341 36 336 1.07e-57 192
MS.gene43036.t1 AT1G77780 33.333 342 212 12 7 343 2 332 1.45e-57 192
MS.gene43036.t1 AT1G77790 33.526 346 214 11 8 346 8 344 1.80e-57 191
MS.gene43036.t1 AT5G20390 34.311 341 210 10 8 343 12 343 1.87e-56 189
MS.gene43036.t1 AT5G20340 33.051 354 210 12 6 343 11 353 3.41e-53 180
MS.gene43036.t1 AT5G20330 32.857 350 208 10 9 343 7 344 4.09e-52 177
MS.gene43036.t1 AT1G33220 30.814 344 212 10 6 343 11 334 3.86e-45 159
MS.gene43036.t1 AT1G64760 31.198 359 222 13 3 348 1 347 6.20e-45 162
MS.gene43036.t1 AT1G64760 31.198 359 222 13 3 348 1 347 6.20e-45 162
MS.gene43036.t1 AT2G19440 32.121 330 207 10 29 348 21 343 9.36e-45 161
MS.gene43036.t1 AT2G19440 32.121 330 207 10 29 348 21 343 9.36e-45 161
MS.gene43036.t1 AT3G55780 32.492 317 194 12 42 347 48 355 1.14e-43 156
MS.gene43036.t1 AT5G18220 29.499 339 223 10 11 341 13 343 2.93e-43 157
MS.gene43036.t1 AT5G64790 30.056 356 219 13 10 351 14 353 3.74e-41 151
MS.gene43036.t1 AT3G04010 31.519 349 219 13 12 348 15 355 5.79e-41 151
MS.gene43036.t1 AT5G58480 31.845 336 202 12 30 349 27 351 4.99e-40 148
MS.gene43036.t1 AT5G20560 29.970 337 218 10 6 335 11 336 2.09e-39 144
MS.gene43036.t1 AT4G31140 28.895 353 231 13 2 345 5 346 2.48e-39 146
MS.gene43036.t1 AT5G20870 32.320 362 212 16 12 350 10 361 2.74e-39 147
MS.gene43036.t1 AT3G24330 29.379 354 226 13 10 348 19 363 1.66e-37 142
MS.gene43036.t1 AT4G17180 30.816 331 205 13 30 348 26 344 7.95e-37 139
MS.gene43036.t1 AT5G58090 28.875 329 217 11 30 347 23 345 8.35e-36 137

Find 63 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGCAATGGTTTGGATTT+TGG 0.082492 2.4:-13742156 MS.gene43036:CDS
TACATTAATTGAAGTGATTT+TGG 0.138128 2.4:+13741897 None:intergenic
ATGCTGTTTCTCACCGTTTC+AGG 0.170134 2.4:-13742504 MS.gene43036:intron
TCTCTACATTCTTACATTCT+TGG 0.176614 2.4:-13740883 MS.gene43036:CDS
ACCCATGATTGTGCCTTAAT+AGG 0.222049 2.4:+13741952 None:intergenic
TACATTCTTGGTGTTTGTTT+GGG 0.256184 2.4:-13740871 MS.gene43036:CDS
TTACATTCTTGGTGTTTGTT+TGG 0.257209 2.4:-13740872 MS.gene43036:CDS
GAGAATGTTGTTAGAACATT+TGG 0.274660 2.4:+13742030 None:intergenic
TGCTGTTTACGCTGCTATAA+AGG 0.295122 2.4:-13741491 MS.gene43036:CDS
TATAATGCACTTGTTAATCT+TGG 0.303970 2.4:-13741805 MS.gene43036:CDS
TGCATTATACACTGATTTCA+TGG 0.317483 2.4:+13741819 None:intergenic
TCAAATGTTGAATTCATGAT+AGG 0.342689 2.4:-13742003 MS.gene43036:CDS
CCCATGATTGTGCCTTAATA+GGG 0.349920 2.4:+13741953 None:intergenic
TTCAATCCAAGGTTATAAAC+TGG 0.351246 2.4:+13741208 None:intergenic
TTGCCTTCAATAATCATTCC+TGG 0.356449 2.4:+13741157 None:intergenic
AATTCATTCCCTCCTTCATC+TGG 0.362933 2.4:-13741730 MS.gene43036:CDS
AAGTATGAAAGACCCTATTA+AGG 0.379469 2.4:-13741965 MS.gene43036:CDS
TGCAGGAGTTCCTTGTTTCA+TGG 0.387844 2.4:+13741339 None:intergenic
TAAATATCAATAGGAATAGA+AGG 0.399395 2.4:+13741313 None:intergenic
GTTTATGCAAAGCAATGGTT+TGG 0.402259 2.4:-13742163 MS.gene43036:CDS
AATGTAGAGAACAAAATTGA+TGG 0.411079 2.4:+13740896 None:intergenic
GCGAAAACATAAATATCAAT+AGG 0.415882 2.4:+13741304 None:intergenic
CCTGCATCAGAGAGAAACTA+TGG 0.421805 2.4:-13741253 MS.gene43036:CDS
AGTGAAGCTCCAACCTCATC+AGG 0.429944 2.4:+13741406 None:intergenic
ATCATAATAATTTGTTGAAG+AGG 0.447117 2.4:-13741366 MS.gene43036:CDS
TTTCCAGGAATGATTATTGA+AGG 0.454929 2.4:-13741160 MS.gene43036:CDS
TTATACACTGATTTCATGGC+TGG 0.455792 2.4:+13741823 None:intergenic
TCATCAGGATCACCTTTAGA+AGG 0.456945 2.4:+13741421 None:intergenic
GGTACTCCAGTTTATAACCT+TGG 0.457524 2.4:-13741214 MS.gene43036:CDS
CCCTATTAAGGCACAATCAT+GGG 0.483365 2.4:-13741953 MS.gene43036:CDS
ACCCTATTAAGGCACAATCA+TGG 0.484483 2.4:-13741954 MS.gene43036:CDS
TCTCTAAATGCACCAGATGA+AGG 0.485251 2.4:+13741718 None:intergenic
TTTAACGAAGATTTGAAGAC+TGG 0.490478 2.4:-13741277 MS.gene43036:CDS
GAGACAGGTTGGCCTTCTAA+AGG 0.497141 2.4:-13741433 MS.gene43036:CDS
TCATAATGCAAATTTGTAGC+AGG 0.507641 2.4:+13741538 None:intergenic
CTGGAAAATAACCTCCTTGA+GGG 0.515738 2.4:+13741176 None:intergenic
AGTAAAAGACTAACCTGAAA+CGG 0.517656 2.4:+13742491 None:intergenic
TTTAGAAGGCCAACCTGTCT+CGG 0.517858 2.4:+13741435 None:intergenic
GAGGTTAAGATTTCCGAGAC+AGG 0.525013 2.4:-13741448 MS.gene43036:CDS
CCATAGTTTCTCTCTGATGC+AGG 0.528755 2.4:+13741253 None:intergenic
AAAATCACTTCAATTAATGT+AGG 0.530035 2.4:-13741895 MS.gene43036:CDS
TTATAAACTGGAGTACCATC+AGG 0.532992 2.4:+13741220 None:intergenic
TGCGCTTGAAAGCTAAGAAG+TGG 0.540232 2.4:+13741673 None:intergenic
TATGGTCTTTATTATCCTGA+TGG 0.543216 2.4:-13741235 MS.gene43036:CDS
CATTATCACTCTGAAGAAGA+AGG 0.546977 2.4:+13742524 None:intergenic
TAGTTTAATGAAACATGTTG+TGG 0.553999 2.4:+13741595 None:intergenic
GTCTTGTTTCAACCGAACCC+TGG 0.554364 2.4:-13741571 MS.gene43036:CDS
GGATCAATAGAACCAGGGTT+CGG 0.558568 2.4:+13741559 None:intergenic
AAAGGTGATCCTGATGAGGT+TGG 0.562864 2.4:-13741415 MS.gene43036:CDS
AAGAGGATTGCCATGAAACA+AGG 0.577245 2.4:-13741349 MS.gene43036:CDS
GATTTGTTTATGCAAAGCAA+TGG 0.583237 2.4:-13742168 MS.gene43036:CDS
GTAGCAGGATCAATAGAACC+AGG 0.591805 2.4:+13741553 None:intergenic
TTAAGATTTCCGAGACAGGT+TGG 0.593135 2.4:-13741444 MS.gene43036:CDS
CTAAATGCACCAGATGAAGG+AGG 0.616012 2.4:+13741721 None:intergenic
TACGCTGCTATAAAGGCGGT+AGG 0.619683 2.4:-13741484 MS.gene43036:CDS
TTCTAAAGGTGATCCTGATG+AGG 0.626238 2.4:-13741419 MS.gene43036:CDS
CCTGGAAAATAACCTCCTTG+AGG 0.629807 2.4:+13741175 None:intergenic
TAAATGCACCAGATGAAGGA+GGG 0.634421 2.4:+13741722 None:intergenic
ACTGCTACACGTGAATGAGA+TGG 0.641775 2.4:+13742102 None:intergenic
ACGCTGCTATAAAGGCGGTA+GGG 0.646566 2.4:-13741483 MS.gene43036:CDS
TAGCAGGATCAATAGAACCA+GGG 0.673870 2.4:+13741554 None:intergenic
GGTAGGGCATAGCGATATCG+AGG 0.719545 2.4:-13741467 MS.gene43036:CDS
TGTTTACGCTGCTATAAAGG+CGG 0.719749 2.4:-13741488 MS.gene43036:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGAAATAAAAATAATATG+TGG + chr2.4:13740954-13740973 None:intergenic 10.0%
!! AAACTAAAATATACTTACAT+TGG + chr2.4:13742262-13742281 None:intergenic 15.0%
!! AAAATCACTTCAATTAATGT+AGG - chr2.4:13741496-13741515 MS.gene43036:CDS 20.0%
!! TAAATATCAATAGGAATAGA+AGG + chr2.4:13742081-13742100 None:intergenic 20.0%
!! TTCAACAAAATTGTTAAAAC+AGG + chr2.4:13741131-13741150 None:intergenic 20.0%
!!! ATCATAATAATTTGTTGAAG+AGG - chr2.4:13742025-13742044 MS.gene43036:CDS 20.0%
!!! TACATTAATTGAAGTGATTT+TGG + chr2.4:13741497-13741516 None:intergenic 20.0%
! AATGTAGAGAACAAAATTGA+TGG + chr2.4:13742498-13742517 None:intergenic 25.0%
! GCGAAAACATAAATATCAAT+AGG + chr2.4:13742090-13742109 None:intergenic 25.0%
! TAGTTTAATGAAACATGTTG+TGG + chr2.4:13741799-13741818 None:intergenic 25.0%
! TCAAATGTTGAATTCATGAT+AGG - chr2.4:13741388-13741407 MS.gene43036:CDS 25.0%
!! AATGTAGGAAATGAAGTTTT+AGG - chr2.4:13741511-13741530 MS.gene43036:CDS 25.0%
!! TATAATGCACTTGTTAATCT+TGG - chr2.4:13741586-13741605 MS.gene43036:CDS 25.0%
!!! TATCCTTTTTTTGCATACAA+AGG - chr2.4:13741769-13741788 MS.gene43036:CDS 25.0%
!!! TATTTTCTGAGTATTGTAAG+CGG + chr2.4:13741041-13741060 None:intergenic 25.0%
!!! TCCAATTTTTGTTAATTTGC+AGG - chr2.4:13742466-13742485 MS.gene43036:intron 25.0%
!!! TTGGATTTTGGAATAAACTA+TGG - chr2.4:13741247-13741266 MS.gene43036:CDS 25.0%
AAGTATGAAAGACCCTATTA+AGG - chr2.4:13741426-13741445 MS.gene43036:CDS 30.0%
AGTAAAAGACTAACCTGAAA+CGG + chr2.4:13740903-13740922 None:intergenic 30.0%
GAGAATGTTGTTAGAACATT+TGG + chr2.4:13741364-13741383 None:intergenic 30.0%
GCCTGCAAATTAACAAAAAT+TGG + chr2.4:13742470-13742489 None:intergenic 30.0%
TATGGTCTTTATTATCCTGA+TGG - chr2.4:13742156-13742175 MS.gene43036:CDS 30.0%
TCACCTTTGTATGCAAAAAA+AGG + chr2.4:13741775-13741794 None:intergenic 30.0%
TCATAATGCAAATTTGTAGC+AGG + chr2.4:13741856-13741875 None:intergenic 30.0%
TCTCTACATTCTTACATTCT+TGG - chr2.4:13742508-13742527 MS.gene43036:CDS 30.0%
TTCAATCCAAGGTTATAAAC+TGG + chr2.4:13742186-13742205 None:intergenic 30.0%
TTTAACGAAGATTTGAAGAC+TGG - chr2.4:13742114-13742133 MS.gene43036:CDS 30.0%
TTTCCAGGAATGATTATTGA+AGG - chr2.4:13742231-13742250 MS.gene43036:intron 30.0%
! GATTTGTTTATGCAAAGCAA+TGG - chr2.4:13741223-13741242 MS.gene43036:CDS 30.0%
!! ATAAGTGCTGAATATATCTG+TGG - chr2.4:13740987-13741006 MS.gene43036:intron 30.0%
!! GGATAAGCATTGATCAAAAA+AGG + chr2.4:13741754-13741773 None:intergenic 30.0%
!! TACATTCTTGGTGTTTGTTT+GGG - chr2.4:13742520-13742539 MS.gene43036:CDS 30.0%
!! TGCATTATACACTGATTTCA+TGG + chr2.4:13741575-13741594 None:intergenic 30.0%
!! TTACATTCTTGGTGTTTGTT+TGG - chr2.4:13742519-13742538 MS.gene43036:CDS 30.0%
!!! ATTGAAGTGATTTTGGTTTG+TGG + chr2.4:13741490-13741509 None:intergenic 30.0%
AATCTTAGACCTTCCGATTA+TGG - chr2.4:13742323-13742342 MS.gene43036:intron 35.0%
GACATCAAATGAGCCATAAT+CGG + chr2.4:13742339-13742358 None:intergenic 35.0%
GTTTATGCAAAGCAATGGTT+TGG - chr2.4:13741228-13741247 MS.gene43036:CDS 35.0%
TTGAAAAATCAAGTCCCTCA+AGG - chr2.4:13742201-13742220 MS.gene43036:intron 35.0%
TTGCCTTCAATAATCATTCC+TGG + chr2.4:13742237-13742256 None:intergenic 35.0%
! ATAGGAATAGAAGGTTTTGC+AGG + chr2.4:13742072-13742091 None:intergenic 35.0%
! CATTATCACTCTGAAGAAGA+AGG + chr2.4:13740870-13740889 None:intergenic 35.0%
! TTATAAACTGGAGTACCATC+AGG + chr2.4:13742174-13742193 None:intergenic 35.0%
!! CAAAGCAATGGTTTGGATTT+TGG - chr2.4:13741235-13741254 MS.gene43036:CDS 35.0%
!! GGACTTGATTTTTCAATCCA+AGG + chr2.4:13742197-13742216 None:intergenic 35.0%
!! TTATACACTGATTTCATGGC+TGG + chr2.4:13741571-13741590 None:intergenic 35.0%
!!! AAGTGATTTTGGTTTGTGGA+AGG + chr2.4:13741486-13741505 None:intergenic 35.0%
!!! ATTTTGGTTTGTGGAAGGTA+TGG + chr2.4:13741481-13741500 None:intergenic 35.0%
AAAAATCAAGTCCCTCAAGG+AGG - chr2.4:13742204-13742223 MS.gene43036:intron 40.0%
AAGAGGATTGCCATGAAACA+AGG - chr2.4:13742042-13742061 MS.gene43036:CDS 40.0%
AATTCATTCCCTCCTTCATC+TGG - chr2.4:13741661-13741680 MS.gene43036:CDS 40.0%
ACCCATGATTGTGCCTTAAT+AGG + chr2.4:13741442-13741461 None:intergenic 40.0%
ACCCTATTAAGGCACAATCA+TGG - chr2.4:13741437-13741456 MS.gene43036:CDS 40.0%
CCCATGATTGTGCCTTAATA+GGG + chr2.4:13741441-13741460 None:intergenic 40.0%
CCCTATTAAGGCACAATCAT+GGG - chr2.4:13741438-13741457 MS.gene43036:CDS 40.0%
CTGGAAAATAACCTCCTTGA+GGG + chr2.4:13742218-13742237 None:intergenic 40.0%
GGTACTCCAGTTTATAACCT+TGG - chr2.4:13742177-13742196 MS.gene43036:intron 40.0%
TAAATGCACCAGATGAAGGA+GGG + chr2.4:13741672-13741691 None:intergenic 40.0%
TAGCAGGATCAATAGAACCA+GGG + chr2.4:13741840-13741859 None:intergenic 40.0%
TCAAATGAGCCATAATCGGA+AGG + chr2.4:13742335-13742354 None:intergenic 40.0%
TCATCAGGATCACCTTTAGA+AGG + chr2.4:13741973-13741992 None:intergenic 40.0%
TCTCTAAATGCACCAGATGA+AGG + chr2.4:13741676-13741695 None:intergenic 40.0%
TGGTATGAATCGTGACAGTA+AGG - chr2.4:13741007-13741026 MS.gene43036:intron 40.0%
TGTTTACGCTGCTATAAAGG+CGG - chr2.4:13741903-13741922 MS.gene43036:CDS 40.0%
TTAAGATTTCCGAGACAGGT+TGG - chr2.4:13741947-13741966 MS.gene43036:CDS 40.0%
! TCTGAAACAGACTACTCGAT+TGG + chr2.4:13742372-13742391 None:intergenic 40.0%
! TGCTGTTTACGCTGCTATAA+AGG - chr2.4:13741900-13741919 MS.gene43036:CDS 40.0%
! TTCTAAAGGTGATCCTGATG+AGG - chr2.4:13741972-13741991 MS.gene43036:CDS 40.0%
AAAGGTGATCCTGATGAGGT+TGG - chr2.4:13741976-13741995 MS.gene43036:CDS 45.0%
ACTGCTACACGTGAATGAGA+TGG + chr2.4:13741292-13741311 None:intergenic 45.0%
CCATAGTTTCTCTCTGATGC+AGG + chr2.4:13742141-13742160 None:intergenic 45.0%
CCTGCATCAGAGAGAAACTA+TGG - chr2.4:13742138-13742157 MS.gene43036:CDS 45.0%
CCTGGAAAATAACCTCCTTG+AGG + chr2.4:13742219-13742238 None:intergenic 45.0%
CTAAATGCACCAGATGAAGG+AGG + chr2.4:13741673-13741692 None:intergenic 45.0%
GAGGTTAAGATTTCCGAGAC+AGG - chr2.4:13741943-13741962 MS.gene43036:CDS 45.0%
GGATCAATAGAACCAGGGTT+CGG + chr2.4:13741835-13741854 None:intergenic 45.0%
GTAGCAGGATCAATAGAACC+AGG + chr2.4:13741841-13741860 None:intergenic 45.0%
TGCGCTTGAAAGCTAAGAAG+TGG + chr2.4:13741721-13741740 None:intergenic 45.0%
TTTAGAAGGCCAACCTGTCT+CGG + chr2.4:13741959-13741978 None:intergenic 45.0%
! TGCAGGAGTTCCTTGTTTCA+TGG + chr2.4:13742055-13742074 None:intergenic 45.0%
!! ATGCTGTTTCTCACCGTTTC+AGG - chr2.4:13740887-13740906 MS.gene43036:CDS 45.0%
!! CCTCAAGGAGGTTATTTTCC+AGG - chr2.4:13742216-13742235 MS.gene43036:intron 45.0%
ACGCTGCTATAAAGGCGGTA+GGG - chr2.4:13741908-13741927 MS.gene43036:CDS 50.0%
ACTACTCGATTGGTGAGCTG+AGG + chr2.4:13742362-13742381 None:intergenic 50.0%
AGTGAAGCTCCAACCTCATC+AGG + chr2.4:13741988-13742007 None:intergenic 50.0%
CTACTCGATTGGTGAGCTGA+GGG + chr2.4:13742361-13742380 None:intergenic 50.0%
TACGCTGCTATAAAGGCGGT+AGG - chr2.4:13741907-13741926 MS.gene43036:CDS 50.0%
! GAGTATTGTAAGCGGCAGAG+TGG + chr2.4:13741033-13741052 None:intergenic 50.0%
!! GAGACAGGTTGGCCTTCTAA+AGG - chr2.4:13741958-13741977 MS.gene43036:CDS 50.0%
!! GTCTTGTTTCAACCGAACCC+TGG - chr2.4:13741820-13741839 MS.gene43036:CDS 50.0%
GGTAGGGCATAGCGATATCG+AGG - chr2.4:13741924-13741943 MS.gene43036:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 13740854 13742559 13740854 ID=MS.gene43036
chr2.4 mRNA 13740854 13742559 13740854 ID=MS.gene43036.t1;Parent=MS.gene43036
chr2.4 exon 13742505 13742559 13742505 ID=MS.gene43036.t1.exon1;Parent=MS.gene43036.t1
chr2.4 CDS 13742505 13742559 13742505 ID=cds.MS.gene43036.t1;Parent=MS.gene43036.t1
chr2.4 exon 13741150 13742189 13741150 ID=MS.gene43036.t1.exon2;Parent=MS.gene43036.t1
chr2.4 CDS 13741150 13742189 13741150 ID=cds.MS.gene43036.t1;Parent=MS.gene43036.t1
chr2.4 exon 13740854 13740925 13740854 ID=MS.gene43036.t1.exon3;Parent=MS.gene43036.t1
chr2.4 CDS 13740854 13740925 13740854 ID=cds.MS.gene43036.t1;Parent=MS.gene43036.t1
Gene Sequence

>MS.gene43036

ATGGCAATCTCTTCCTTCTTCTTCAGAGTGATAATGCTGTTTCTCACCGTTTCAGGTTAGTCTTTTACTTCTTCTGCGTGCATGATTCTCTTTCATACCACATATTATTTTTATTTCATAACGAATCGAAGCGATAAGTGCTGAATATATCTGTGGTATGAATCGTGACAGTAAGGCCACTCTGCCGCTTACAATACTCAGAAAATAAAAACTACTCTTTTATTGTCAAGTAATCTTTATTTTGCACTTTTTTTTGCTTGAATTTGATATTTTACCTGTTTTAACAATTTTGTTGAATTAGATATTTGTTCTACTAAATTAAATTTAATGAACAAATTTTGATATTTCAAACTTTTTGTTCTTTTTTCAGATTTGTTTATGCAAAGCAATGGTTTGGATTTTGGAATAAACTATGGACAAATAGCAAACAATTTACCATCTCATTCACGTGTAGCAGTTCTTATAAAATCATTGAATGTGAGCAGAATCAAACTCTATGATGCTGATCCAAATGTTCTAACAACATTCTCAAATTCAAATGTTGAATTCATGATAGGACTTAATGATCTTCAAAGTATGAAAGACCCTATTAAGGCACAATCATGGGTTCAACAAAATGTTCAACCATACCTTCCACAAACCAAAATCACTTCAATTAATGTAGGAAATGAAGTTTTAGGTAACAATGATATAAACAGTTATAATAATCTTCTTCCAGCCATGAAATCAGTGTATAATGCACTTGTTAATCTTGGATTATCTCAACAAGTTACTGTCACAACTTCACATTCTTTCATCATTATGTCAAATTCATTCCCTCCTTCATCTGGTGCATTTAGAGAAGATTTGATACAATATATTCAACCACTTCTTAGCTTTCAAGCGCAAATCAAATCGCCTTTTTTGATCAATGCTTATCCTTTTTTTGCATACAAAGGTGATCCACAACATGTTTCATTAAACTATGTCTTGTTTCAACCGAACCCTGGTTCTATTGATCCTGCTACAAATTTGCATTATGATAACATGTTGTATGCTCAAATCGATGCTGTTTACGCTGCTATAAAGGCGGTAGGGCATAGCGATATCGAGGTTAAGATTTCCGAGACAGGTTGGCCTTCTAAAGGTGATCCTGATGAGGTTGGAGCTTCACTACAGAATGCAGAAATTTATCATAATAATTTGTTGAAGAGGATTGCCATGAAACAAGGAACTCCTGCAAAACCTTCTATTCCTATTGATATTTATGTTTTCGCACTTTTTAACGAAGATTTGAAGACTGGTCCTGCATCAGAGAGAAACTATGGTCTTTATTATCCTGATGGTACTCCAGTTTATAACCTTGGATTGAAAAATCAAGTCCCTCAAGGAGGTTATTTTCCAGGAATGATTATTGAAGGCAAATCCAATGTAAGTATATTTTAGTTTGTGTTAGTAAATTTGTAGATTATGTTAGTTTTCAGAGTTTGAATCTTAGACCTTCCGATTATGGCTCATTTGATGTCCCTCAGCTCACCAATCGAGTAGTCTGTTTCAGATATTATCTTTTGTAATTAACATTTTTGTAGAGTATATTACAATATGACTGAGTAATATTTGATGTATCTAACTCTCCAATTTTTGTTAATTTGCAGGCTTTGTCCATCAATTTTGTTCTCTACATTCTTACATTCTTGGTGTTTGTTTGGGAGCTTTCAATATTGTGA
Protein sequence

>MS.gene43036.t1

MAISSFFFRVIMLFLTVSDLFMQSNGLDFGINYGQIANNLPSHSRVAVLIKSLNVSRIKLYDADPNVLTTFSNSNVEFMIGLNDLQSMKDPIKAQSWVQQNVQPYLPQTKITSINVGNEVLGNNDINSYNNLLPAMKSVYNALVNLGLSQQVTVTTSHSFIIMSNSFPPSSGAFREDLIQYIQPLLSFQAQIKSPFLINAYPFFAYKGDPQHVSLNYVLFQPNPGSIDPATNLHYDNMLYAQIDAVYAAIKAVGHSDIEVKISETGWPSKGDPDEVGASLQNAEIYHNNLLKRIAMKQGTPAKPSIPIDIYVFALFNEDLKTGPASERNYGLYYPDGTPVYNLGLKNQVPQGGYFPGMIIEGKSNALSINFVLYILTFLVFVWELSIL