AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43469

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43469.t1 MTR_4g134490 97.222 360 10 0 1 360 14 373 0.0 733
MS.gene43469.t1 MTR_4g134490 95.628 366 10 1 1 360 14 379 0.0 727
MS.gene43469.t1 MTR_8g096880 79.130 345 72 0 9 353 3 347 0.0 591
MS.gene43469.t1 MTR_8g096880 78.505 321 69 0 9 329 3 323 0.0 540
MS.gene43469.t1 MTR_8g096880 78.425 292 63 0 9 300 3 294 2.45e-173 484
MS.gene43469.t1 MTR_5g009170 66.860 344 112 2 9 352 44 385 3.07e-173 487
MS.gene43469.t1 MTR_8g099535 67.155 341 110 2 12 352 4 342 6.31e-171 480
MS.gene43469.t1 MTR_5g009160 65.689 341 115 2 12 352 4 342 1.37e-168 474
MS.gene43469.t1 MTR_5g009160 67.658 269 86 1 84 352 12 279 8.35e-137 391
MS.gene43469.t1 MTR_5g009160 66.359 217 72 1 84 300 12 227 7.39e-104 305
MS.gene43469.t1 MTR_2g032100 41.311 351 180 10 14 352 73 409 1.44e-82 257
MS.gene43469.t1 MTR_8g089180 40.510 353 184 10 14 354 73 411 1.55e-78 247
MS.gene43469.t1 MTR_8g089180 40.510 353 184 10 14 354 73 411 1.55e-78 247
MS.gene43469.t1 MTR_2g032100 42.857 196 107 5 14 208 73 264 2.40e-46 160
MS.gene43469.t1 MTR_8g099285 74.545 55 13 1 244 298 3 56 6.35e-21 85.5
MS.gene43469.t1 MTR_1g009600 25.212 353 223 13 10 351 3 325 1.59e-19 90.5
MS.gene43469.t1 MTR_2g073240 24.501 351 224 14 12 351 19 339 4.88e-19 89.0
MS.gene43469.t1 MTR_0034s0060 23.714 350 228 13 12 351 137 457 3.68e-17 83.6
MS.gene43469.t1 MTR_4g102270 25.424 354 234 11 3 344 84 419 1.66e-16 80.9
MS.gene43469.t1 MTR_4g120030 27.596 337 194 13 7 336 106 399 2.04e-16 80.5
MS.gene43469.t1 MTR_4g129820 24.789 355 235 10 3 344 86 421 3.61e-15 76.6
MS.gene43469.t1 MTR_5g011940 24.781 343 230 10 13 344 91 416 4.09e-15 76.6
MS.gene43469.t1 MTR_1g088480 26.970 330 191 13 14 336 120 406 8.94e-14 72.4
MS.gene43469.t1 MTR_1g067530 26.705 352 207 17 11 336 111 437 6.47e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43469.t1 AT1G12780 78.797 349 74 0 8 356 3 351 0.0 593
MS.gene43469.t1 AT1G63180 76.590 346 81 0 8 353 3 348 0.0 580
MS.gene43469.t1 AT4G10960 66.959 342 111 2 12 353 4 343 7.27e-175 490
MS.gene43469.t1 AT4G23920 67.160 338 109 2 12 349 3 338 5.53e-171 480
MS.gene43469.t1 AT1G64440 66.372 339 112 2 14 352 5 341 3.38e-170 478
MS.gene43469.t1 AT1G63180 75.601 291 71 0 8 298 3 293 3.21e-169 474
MS.gene43469.t1 AT5G44480 38.857 350 190 8 14 352 73 409 2.55e-78 246
MS.gene43469.t1 AT5G44480 38.857 350 190 8 14 352 73 409 2.55e-78 246
MS.gene43469.t1 AT5G44480 38.857 350 190 8 14 352 73 409 2.55e-78 246
MS.gene43469.t1 AT5G44480 38.857 350 190 8 14 352 97 433 6.99e-78 246
MS.gene43469.t1 AT4G20460 38.000 350 193 7 14 352 72 408 1.50e-76 242
MS.gene43469.t1 AT1G30620 38.352 352 193 8 14 354 73 411 1.56e-74 237
MS.gene43469.t1 AT1G30620 38.352 352 193 8 14 354 73 411 1.56e-74 237
MS.gene43469.t1 AT1G30620 38.352 352 193 8 14 354 73 411 1.56e-74 237
MS.gene43469.t1 AT1G30620 38.352 352 193 8 14 354 73 411 1.56e-74 237
MS.gene43469.t1 AT1G30620 38.352 352 193 8 14 354 72 410 1.57e-74 237
MS.gene43469.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.25e-42 147
MS.gene43469.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.25e-42 147
MS.gene43469.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.25e-42 147
MS.gene43469.t1 AT2G34850 36.134 238 130 6 126 352 1 227 3.25e-42 147
MS.gene43469.t1 AT1G30620 38.974 195 116 3 14 208 73 264 1.43e-41 147
MS.gene43469.t1 AT1G53500 24.571 350 225 13 12 351 9 329 6.23e-19 88.6
MS.gene43469.t1 AT3G14790 24.370 357 231 12 5 351 3 330 6.64e-19 88.6
MS.gene43469.t1 AT3G14790 24.571 350 225 12 12 351 7 327 8.80e-19 88.2
MS.gene43469.t1 AT3G62830 28.182 330 192 15 14 336 121 412 8.62e-18 84.7
MS.gene43469.t1 AT3G62830 28.182 330 192 15 14 336 121 412 8.62e-18 84.7
MS.gene43469.t1 AT3G53520 27.809 356 208 16 10 354 118 435 2.02e-17 83.6
MS.gene43469.t1 AT2G47650 27.879 330 193 15 14 336 123 414 2.81e-17 83.2
MS.gene43469.t1 AT1G78570 24.786 351 223 14 12 351 7 327 7.17e-17 82.4
MS.gene43469.t1 AT3G53520 27.528 356 207 16 10 354 118 433 1.14e-15 78.2
MS.gene43469.t1 AT2G47650 27.381 336 193 16 14 336 123 420 3.86e-15 76.6
MS.gene43469.t1 AT4G12250 25.581 344 226 11 13 344 97 422 1.24e-14 75.1
MS.gene43469.t1 AT4G30440 25.205 365 223 14 2 344 78 414 2.05e-14 74.3
MS.gene43469.t1 AT3G53520 27.473 364 182 20 10 336 118 436 9.06e-14 72.8
MS.gene43469.t1 AT3G53520 30.159 252 137 13 10 254 118 337 6.78e-11 63.5

Find 121 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTCTGCAGCAGTTTA+TGG 0.176720 8.4:-340922 MS.gene43469:CDS
TTGCCCGAGCTCAACGTTTA+TGG 0.230700 8.4:-340582 MS.gene43469:CDS
TAAGTAAGGCATCAGATTAT+TGG 0.264960 8.4:+340626 None:intergenic
CATAAGGATTCATGGCTTGC+AGG 0.302019 8.4:+340863 None:intergenic
GCTGCATTTGAAAAGGCTTC+TGG 0.308975 8.4:-340223 MS.gene43469:CDS
GGTTCTTCCATAAGGATTCA+TGG 0.337426 8.4:+340855 None:intergenic
TACCAGCTTGAGCTTGGGTT+TGG 0.340617 8.4:-339822 MS.gene43469:CDS
AAGTAAGGCATCAGATTATT+GGG 0.352747 8.4:+340627 None:intergenic
GGTCGTTCAGCTTCTAAAAC+AGG 0.355343 8.4:-341250 MS.gene43469:CDS
CTGCAAGCCATGAATCCTTA+TGG 0.360608 8.4:-340862 MS.gene43469:CDS
TGTCCACAGGGTTCGCCATT+TGG 0.362347 8.4:-341178 MS.gene43469:CDS
GTCGGAAAGGAAAACGATAA+TGG 0.375088 8.4:-341307 MS.gene43469:CDS
ACCAGCTTGAGCTTGGGTTT+GGG 0.376831 8.4:-339821 MS.gene43469:CDS
TGTGCAGAGACCAATGGAAT+TGG 0.380858 8.4:-339866 MS.gene43469:CDS
GCAATCTTCCAACTGCTACT+TGG 0.382864 8.4:+340601 None:intergenic
ACGGGTGGGGCGGGGTTCAT+TGG 0.384071 8.4:-341282 MS.gene43469:CDS
ACTCACCGCAGTGCCATCTT+TGG 0.403250 8.4:+340545 None:intergenic
AGTACCTTGGTTCTTCCATA+AGG 0.405042 8.4:+340847 None:intergenic
TGGGGATACCAGCTTGAGCT+TGG 0.405772 8.4:-339828 MS.gene43469:CDS
ATTGTAGAGATTGTCGATAA+TGG 0.413237 8.4:+341215 None:intergenic
TAATGGTAACGGGTGGGGCG+GGG 0.417054 8.4:-341290 MS.gene43469:CDS
CAATCTTCCAACTGCTACTT+GGG 0.419596 8.4:+340602 None:intergenic
GGGGATACCAGCTTGAGCTT+GGG 0.426150 8.4:-339827 MS.gene43469:CDS
TGAGATACTTCAATCCGGTT+GGG 0.434754 8.4:-340692 MS.gene43469:CDS
GCACATGAGAGCGGTGAGAT+TGG 0.438760 8.4:-340669 MS.gene43469:CDS
TGTTGCTTGCCCAATTCCAT+TGG 0.442181 8.4:+339856 None:intergenic
CCGCTCTCATGTGCCCCAAC+CGG 0.449340 8.4:+340678 None:intergenic
GATAATGGTAACGGGTGGGG+CGG 0.450585 8.4:-341292 MS.gene43469:CDS
TCAACGCAGGGAATTTCATC+TGG 0.454641 8.4:+340895 None:intergenic
CACCGCAGTGCCATCTTTGG+TGG 0.455579 8.4:+340548 None:intergenic
ATAATGGTAACGGGTGGGGC+GGG 0.455901 8.4:-341291 MS.gene43469:CDS
CACAGGGTTCGCCATTTGGT+TGG 0.459853 8.4:-341174 MS.gene43469:CDS
AATGGTTGCTGCATTTGAAA+AGG 0.465118 8.4:-340230 MS.gene43469:CDS
TACAACTTGGGAACTGGCCG+CGG 0.465961 8.4:-340268 MS.gene43469:CDS
ATAGATAGCAGTAGCATCTC+CGG 0.466954 8.4:+340057 None:intergenic
AGATTATTGGGTATGCCCCT+TGG 0.469183 8.4:+340639 None:intergenic
GTGCAGAGACCAATGGAATT+GGG 0.470949 8.4:-339865 MS.gene43469:CDS
AGTGCCTACAACTTGGGAAC+TGG 0.472400 8.4:-340274 MS.gene43469:CDS
TACTTAACCCAAGTAGCAGT+TGG 0.476959 8.4:-340609 MS.gene43469:CDS
AACGATAATGGTAACGGGTG+GGG 0.478155 8.4:-341295 MS.gene43469:CDS
CATAAGGCTGAAAATGAACT+TGG 0.482154 8.4:-340025 MS.gene43469:CDS
CATGATTATCCCACCAAAGA+TGG 0.483974 8.4:-340558 MS.gene43469:CDS
CATCTGCCAGGTCCATTACA+TGG 0.489946 8.4:+340430 None:intergenic
GGTTGTAGTGCCTACAACTT+GGG 0.490332 8.4:-340280 MS.gene43469:CDS
ATTGGGCAAGCAACAATCCA+TGG 0.492922 8.4:-339848 MS.gene43469:CDS
CCGTCATGGAAGCTGTCCAC+AGG 0.495003 8.4:-341191 MS.gene43469:CDS
CGGTACATCAGTGCTTCAAA+TGG 0.497630 8.4:-340248 MS.gene43469:CDS
CTGAGATACTTCAATCCGGT+TGG 0.503362 8.4:-340693 MS.gene43469:CDS
TATCTATGCATCTACGCATA+AGG 0.505409 8.4:-340041 MS.gene43469:CDS
GTGAGATTGGCGAAGATCCA+AGG 0.505565 8.4:-340656 MS.gene43469:CDS
GTGGATCACAGCGTCAAACT+TGG 0.505767 8.4:+341071 None:intergenic
AGGCTGAAAATGAACTTGGT+TGG 0.510946 8.4:-340021 MS.gene43469:CDS
TGACCATAAACGTTGAGCTC+GGG 0.516024 8.4:+340579 None:intergenic
GTAGCATCTCCGGGCCTCCT+CGG 0.516501 8.4:+340067 None:intergenic
ACGGGACTTTATCCATGTAA+TGG 0.518510 8.4:-340442 MS.gene43469:CDS
AGGTTGTAGTGCCTACAACT+TGG 0.522897 8.4:-340281 MS.gene43469:CDS
GCGGCCAGTTCCCAAGTTGT+AGG 0.524177 8.4:+340270 None:intergenic
CAGCAATGTGACCATCTGCC+AGG 0.524516 8.4:+340418 None:intergenic
CTCTGCGACACTTTCTCCCA+CGG 0.527424 8.4:+341032 None:intergenic
ACTGCTACTTGGGTTAAGTA+AGG 0.528316 8.4:+340612 None:intergenic
GAGATACTTCAATCCGGTTG+GGG 0.536252 8.4:-340691 MS.gene43469:CDS
AAAATGTGTCCGAGGAGGCC+CGG 0.536292 8.4:-340076 MS.gene43469:CDS
GACGCTGTGATCCACTTTGG+TGG 0.540445 8.4:-341063 MS.gene43469:CDS
AGGAAAACGATAATGGTAAC+GGG 0.546669 8.4:-341300 MS.gene43469:CDS
TTGATTGACAGGGCAAAATA+CGG 0.547401 8.4:-339900 MS.gene43469:intron
ATGGTGAAAGCGGAGGTAGT+TGG 0.547626 8.4:+341140 None:intergenic
TCTCCAACTCTTGCTTGTTG+CGG 0.553361 8.4:+341109 None:intergenic
ATTGGAAAGAGATGGTGAGT+GGG 0.558504 8.4:-341341 None:intergenic
AAAGTTGAGGACCAACCAAA+TGG 0.558547 8.4:+341163 None:intergenic
GGCAATACCAATCGTCGGAA+AGG 0.560434 8.4:-341320 MS.gene43469:CDS
CTTTGGTGGTCTGAAAGCCG+TGG 0.561091 8.4:-341049 MS.gene43469:CDS
TGAGATTGGCGAAGATCCAA+GGG 0.561571 8.4:-340655 MS.gene43469:CDS
TCTCCGCAACAAGCAAGAGT+TGG 0.561707 8.4:-341112 MS.gene43469:CDS
TTGGGCAAGCAACAATCCAT+GGG 0.563515 8.4:-339847 MS.gene43469:CDS
CAGGGCAAAATACGGTATAG+AGG 0.565533 8.4:-339892 MS.gene43469:CDS
ATTTGAAAAGGCTTCTGGCA+AGG 0.566930 8.4:-340218 MS.gene43469:intron
TCCCAAACCCAAGCTCAAGC+TGG 0.567837 8.4:+339820 None:intergenic
ATTGCTGAGATACTTCAATC+CGG 0.576619 8.4:-340697 MS.gene43469:CDS
ATGACCATAAACGTTGAGCT+CGG 0.577684 8.4:+340578 None:intergenic
GATTGCACGAGATATTCAAA+AGG 0.578799 8.4:-340742 MS.gene43469:CDS
CTTTATCCATGTAATGGACC+TGG 0.579239 8.4:-340436 MS.gene43469:CDS
CATGTAATGGACCTGGCAGA+TGG 0.583826 8.4:-340429 MS.gene43469:CDS
AGCAATATTATCCTCCACTC+TGG 0.589937 8.4:+340714 None:intergenic
TTCAAAAGGCAGAGCCAGAG+TGG 0.592430 8.4:-340728 MS.gene43469:CDS
AGCTCAAGCTGGTATCCCCA+TGG 0.594214 8.4:+339831 None:intergenic
TTTGACGCTGTGATCCACTT+TGG 0.595864 8.4:-341066 MS.gene43469:CDS
CGGAGGTAGTTGGAAAGTTG+AGG 0.595990 8.4:+341150 None:intergenic
TAGATAGCAGTAGCATCTCC+GGG 0.603256 8.4:+340058 None:intergenic
AAACGATAATGGTAACGGGT+GGG 0.605066 8.4:-341296 MS.gene43469:CDS
CAGGTGGAAGTCCTCAACGC+AGG 0.605927 8.4:+340882 None:intergenic
GATTGGAAAGAGATGGTGAG+TGG 0.608418 8.4:-341342 None:intergenic
AGGAGATGTGCAGAGACCAA+TGG 0.609293 8.4:-339872 MS.gene43469:CDS
ACCGCAGTGCCATCTTTGGT+GGG 0.615427 8.4:+340549 None:intergenic
AAGGAAAACGATAATGGTAA+CGG 0.616022 8.4:-341301 MS.gene43469:CDS
CGTCATGGAAGCTGTCCACA+GGG 0.616430 8.4:-341190 MS.gene43469:CDS
AAGGATTCATGGCTTGCAGG+TGG 0.618135 8.4:+340866 None:intergenic
CGGCTTTCAGACCACCAAAG+TGG 0.621055 8.4:+341052 None:intergenic
TTGTGATCAAAATATTTGAG+TGG 0.621926 8.4:+341009 None:intergenic
TTTGGTGGTCTGAAAGCCGT+GGG 0.624204 8.4:-341048 MS.gene43469:CDS
AGGTGGAAGTCCTCAACGCA+GGG 0.631756 8.4:+340883 None:intergenic
AGATGAAATTCCCTGCGTTG+AGG 0.633891 8.4:-340893 MS.gene43469:CDS
CAATCTCTACAATTCCGTCA+TGG 0.635522 8.4:-341205 MS.gene43469:CDS
AAAACGATAATGGTAACGGG+TGG 0.639831 8.4:-341297 MS.gene43469:CDS
CCGGTTGGGGCACATGAGAG+CGG 0.640769 8.4:-340678 MS.gene43469:CDS
TTCCTATTAAAATGTGTCCG+AGG 0.642928 8.4:-340084 MS.gene43469:CDS
GAGTGGGCAATACCAATCGT+CGG 0.643532 8.4:-341325 MS.gene43469:CDS
GTCGTTCAGCTTCTAAAACA+GGG 0.648315 8.4:-341249 MS.gene43469:CDS
AAAAGGCAGAGCCAGAGTGG+AGG 0.653042 8.4:-340725 MS.gene43469:CDS
GGAGATCGACATGGTGAAAG+CGG 0.655928 8.4:+341130 None:intergenic
CTATTAAAATGTGTCCGAGG+AGG 0.658139 8.4:-340081 MS.gene43469:CDS
GCTTGTTGCGGAGATCGACA+TGG 0.660195 8.4:+341121 None:intergenic
TTGACCATAAACTGCTGCAG+AGG 0.664691 8.4:+340918 None:intergenic
CAACCAAATGGCGAACCCTG+TGG 0.665060 8.4:+341175 None:intergenic
GATCGACATGGTGAAAGCGG+AGG 0.669740 8.4:+341133 None:intergenic
CCTGTGGACAGCTTCCATGA+CGG 0.679733 8.4:+341191 None:intergenic
GAATCCTTATGGAAGAACCA+AGG 0.701863 8.4:-340851 MS.gene43469:intron
GGGGTTCATTGGATCACACA+CGG 0.730801 8.4:-341271 MS.gene43469:CDS
TTTGAAGCACTGATGTACCG+CGG 0.744540 8.4:+340251 None:intergenic
TGGGCAAGCAACAATCCATG+GGG 0.753069 8.4:-339846 MS.gene43469:CDS
GAGATTGGCGAAGATCCAAG+GGG 0.771146 8.4:-340654 MS.gene43469:CDS
TCCCACCAAAGATGGCACTG+CGG 0.774633 8.4:-340550 MS.gene43469:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAATACTGTTATTATT+AGG - chr8.4:340841-340860 MS.gene43469:intron 10.0%
!!! ATCATTATTCATTATTTTAA+TGG + chr8.4:340336-340355 None:intergenic 10.0%
!! ATAAATAGTTAAATGTAAGT+AGG + chr8.4:340790-340809 None:intergenic 15.0%
!! AGAATACAATACAATACATT+CGG + chr8.4:340995-341014 None:intergenic 20.0%
!! AGTACTTTACTATATTTCAT+TGG - chr8.4:341149-341168 MS.gene43469:CDS 20.0%
!! GTACTTTACTATATTTCATT+GGG - chr8.4:341150-341169 MS.gene43469:CDS 20.0%
!! TAAATTTCAGTGTAAAAAGA+TGG - chr8.4:340192-340211 MS.gene43469:intron 20.0%
!!! ACATTTTAATAGGAATTTTC+TGG + chr8.4:341053-341072 None:intergenic 20.0%
! AACAGTATTTAATTAAGTCC+TGG + chr8.4:340835-340854 None:intergenic 25.0%
! ACAATATATGAATCAGATAC+GGG - chr8.4:340682-340701 MS.gene43469:CDS 25.0%
! ATGAATAATGATACTTAAGC+TGG - chr8.4:340344-340363 MS.gene43469:intron 25.0%
! GACAATATATGAATCAGATA+CGG - chr8.4:340681-340700 MS.gene43469:CDS 25.0%
! TCAGTATCAGAATATATGTT+GGG + chr8.4:340663-340682 None:intergenic 25.0%
! TTATTTACGATTGATTGACA+GGG - chr8.4:341232-341251 MS.gene43469:CDS 25.0%
! TTGTGATCAAAATATTTGAG+TGG + chr8.4:340136-340155 None:intergenic 25.0%
! TTTATTTACGATTGATTGAC+AGG - chr8.4:341231-341250 MS.gene43469:CDS 25.0%
!!! CAAATATTTTGATCACAACT+TGG - chr8.4:340138-340157 MS.gene43469:intron 25.0%
AGTCATCACTTGAAAAAGAT+TGG + chr8.4:340173-340192 None:intergenic 30.0%
ATATACTGAGTTAAGTACCT+TGG + chr8.4:340311-340330 None:intergenic 30.0%
ATTGTAGAGATTGTCGATAA+TGG + chr8.4:339930-339949 None:intergenic 30.0%
CTTTACTATATTTCATTGGG+AGG - chr8.4:341153-341172 MS.gene43469:CDS 30.0%
GTCAGTATCAGAATATATGT+TGG + chr8.4:340664-340683 None:intergenic 30.0%
! AAGGAAAACGATAATGGTAA+CGG - chr8.4:339841-339860 MS.gene43469:CDS 30.0%
! AAGTAAGGCATCAGATTATT+GGG + chr8.4:340518-340537 None:intergenic 30.0%
! TAAGTAAGGCATCAGATTAT+TGG + chr8.4:340519-340538 None:intergenic 30.0%
!!! TATTTTGATCACAACTTGGT+TGG - chr8.4:340142-340161 MS.gene43469:intron 30.0%
AATATATGTTGGGTGCTAAC+TGG + chr8.4:340653-340672 None:intergenic 35.0%
AATGGTTGCTGCATTTGAAA+AGG - chr8.4:340912-340931 MS.gene43469:CDS 35.0%
ACTATATTTCATTGGGAGGA+AGG - chr8.4:341157-341176 MS.gene43469:CDS 35.0%
ATTGCTGAGATACTTCAATC+CGG - chr8.4:340445-340464 MS.gene43469:CDS 35.0%
ATTGTAGATTAGAGCTAACC+AGG - chr8.4:340814-340833 MS.gene43469:intron 35.0%
CATAAGGCTGAAAATGAACT+TGG - chr8.4:341117-341136 MS.gene43469:CDS 35.0%
CTATATTTCATTGGGAGGAA+GGG - chr8.4:341158-341177 MS.gene43469:CDS 35.0%
GATTGCACGAGATATTCAAA+AGG - chr8.4:340400-340419 MS.gene43469:CDS 35.0%
TATCTATGCATCTACGCATA+AGG - chr8.4:341101-341120 MS.gene43469:CDS 35.0%
TGTATTCTTGATCCTTCGTT+TGG - chr8.4:341007-341026 MS.gene43469:CDS 35.0%
TTCCTATTAAAATGTGTCCG+AGG - chr8.4:341058-341077 MS.gene43469:CDS 35.0%
TTGATTGACAGGGCAAAATA+CGG - chr8.4:341242-341261 MS.gene43469:CDS 35.0%
! AGGAAAACGATAATGGTAAC+GGG - chr8.4:339842-339861 MS.gene43469:CDS 35.0%
! CTTTTTGCGACAGACAAAAT+CGG - chr8.4:340758-340777 MS.gene43469:CDS 35.0%
! TGATATATGACAGCTTTTCG+CGG - chr8.4:340373-340392 MS.gene43469:intron 35.0%
AAAGTTGAGGACCAACCAAA+TGG + chr8.4:339982-340001 None:intergenic 40.0%
ACGGGACTTTATCCATGTAA+TGG - chr8.4:340700-340719 MS.gene43469:CDS 40.0%
AGCAATATTATCCTCCACTC+TGG + chr8.4:340431-340450 None:intergenic 40.0%
AGGCTGAAAATGAACTTGGT+TGG - chr8.4:341121-341140 MS.gene43469:CDS 40.0%
ATAGATAGCAGTAGCATCTC+CGG + chr8.4:341088-341107 None:intergenic 40.0%
ATGACCATAAACGTTGAGCT+CGG + chr8.4:340567-340586 None:intergenic 40.0%
ATTTGAAAAGGCTTCTGGCA+AGG - chr8.4:340924-340943 MS.gene43469:CDS 40.0%
CAATCTCTACAATTCCGTCA+TGG - chr8.4:339937-339956 MS.gene43469:intron 40.0%
CATGATTATCCCACCAAAGA+TGG - chr8.4:340584-340603 MS.gene43469:CDS 40.0%
CTATTAAAATGTGTCCGAGG+AGG - chr8.4:341061-341080 MS.gene43469:CDS 40.0%
GAATCCTTATGGAAGAACCA+AGG - chr8.4:340291-340310 MS.gene43469:intron 40.0%
GGTTCTTCCATAAGGATTCA+TGG + chr8.4:340290-340309 None:intergenic 40.0%
GTCGGAAAGGAAAACGATAA+TGG - chr8.4:339835-339854 MS.gene43469:CDS 40.0%
TACTTAACCCAAGTAGCAGT+TGG - chr8.4:340533-340552 MS.gene43469:intron 40.0%
TCTGGAAAACAGATCCAAGA+AGG + chr8.4:341035-341054 None:intergenic 40.0%
TGAGATACTTCAATCCGGTT+GGG - chr8.4:340450-340469 MS.gene43469:intron 40.0%
! ACTGCTACTTGGGTTAAGTA+AGG + chr8.4:340533-340552 None:intergenic 40.0%
! AGTACCTTGGTTCTTCCATA+AGG + chr8.4:340298-340317 None:intergenic 40.0%
! CAATCTTCCAACTGCTACTT+GGG + chr8.4:340543-340562 None:intergenic 40.0%
!! AAAACGATAATGGTAACGGG+TGG - chr8.4:339845-339864 MS.gene43469:CDS 40.0%
!! AAACGATAATGGTAACGGGT+GGG - chr8.4:339846-339865 MS.gene43469:CDS 40.0%
!! CTCCTCGGACACATTTTAAT+AGG + chr8.4:341063-341082 None:intergenic 40.0%
!! CTTTATCCATGTAATGGACC+TGG - chr8.4:340706-340725 MS.gene43469:CDS 40.0%
!! GTCGTTCAGCTTCTAAAACA+GGG - chr8.4:339893-339912 MS.gene43469:intron 40.0%
AGATGAAATTCCCTGCGTTG+AGG - chr8.4:340249-340268 MS.gene43469:CDS 45.0%
AGATTATTGGGTATGCCCCT+TGG + chr8.4:340506-340525 None:intergenic 45.0%
AGGTTGTAGTGCCTACAACT+TGG - chr8.4:340861-340880 MS.gene43469:CDS 45.0%
ATTGGGCAAGCAACAATCCA+TGG - chr8.4:341294-341313 MS.gene43469:CDS 45.0%
CAGGGCAAAATACGGTATAG+AGG - chr8.4:341250-341269 MS.gene43469:CDS 45.0%
CGGTACATCAGTGCTTCAAA+TGG - chr8.4:340894-340913 MS.gene43469:CDS 45.0%
CTGAGATACTTCAATCCGGT+TGG - chr8.4:340449-340468 MS.gene43469:intron 45.0%
CTGCAAGCCATGAATCCTTA+TGG - chr8.4:340280-340299 MS.gene43469:CDS 45.0%
GAGATACTTCAATCCGGTTG+GGG - chr8.4:340451-340470 MS.gene43469:intron 45.0%
GCAATCTTCCAACTGCTACT+TGG + chr8.4:340544-340563 None:intergenic 45.0%
GCTGCATTTGAAAAGGCTTC+TGG - chr8.4:340919-340938 MS.gene43469:CDS 45.0%
GGTTGTAGTGCCTACAACTT+GGG - chr8.4:340862-340881 MS.gene43469:CDS 45.0%
GTGCAGAGACCAATGGAATT+GGG - chr8.4:341277-341296 MS.gene43469:CDS 45.0%
TAGATAGCAGTAGCATCTCC+GGG + chr8.4:341087-341106 None:intergenic 45.0%
TATTTCATTGGGAGGAAGGG+AGG - chr8.4:341161-341180 MS.gene43469:CDS 45.0%
TCAACGCAGGGAATTTCATC+TGG + chr8.4:340250-340269 None:intergenic 45.0%
TCATCCTCTGCAGCAGTTTA+TGG - chr8.4:340220-340239 MS.gene43469:CDS 45.0%
TGACCATAAACGTTGAGCTC+GGG + chr8.4:340566-340585 None:intergenic 45.0%
TGAGATTGGCGAAGATCCAA+GGG - chr8.4:340487-340506 MS.gene43469:intron 45.0%
TGTGCAGAGACCAATGGAAT+TGG - chr8.4:341276-341295 MS.gene43469:CDS 45.0%
TTGACCATAAACTGCTGCAG+AGG + chr8.4:340227-340246 None:intergenic 45.0%
TTGCGACAGACAAAATCGGT+AGG - chr8.4:340762-340781 MS.gene43469:intron 45.0%
TTGGGCAAGCAACAATCCAT+GGG - chr8.4:341295-341314 MS.gene43469:CDS 45.0%
TTTGACGCTGTGATCCACTT+TGG - chr8.4:340076-340095 MS.gene43469:CDS 45.0%
! CATAAGGATTCATGGCTTGC+AGG + chr8.4:340282-340301 None:intergenic 45.0%
! TCGTTTTCCTTTCCGACGAT+TGG + chr8.4:339832-339851 None:intergenic 45.0%
! TCTCCAACTCTTGCTTGTTG+CGG + chr8.4:340036-340055 None:intergenic 45.0%
! TGTTGCTTGCCCAATTCCAT+TGG + chr8.4:341289-341308 None:intergenic 45.0%
!! AACGATAATGGTAACGGGTG+GGG - chr8.4:339847-339866 MS.gene43469:CDS 45.0%
!! GGTCGTTCAGCTTCTAAAAC+AGG - chr8.4:339892-339911 MS.gene43469:CDS 45.0%
!! TTTGAAGCACTGATGTACCG+CGG + chr8.4:340894-340913 None:intergenic 45.0%
ACCAGCTTGAGCTTGGGTTT+GGG - chr8.4:341321-341340 MS.gene43469:CDS 50.0%
AGGAGATGTGCAGAGACCAA+TGG - chr8.4:341270-341289 MS.gene43469:CDS 50.0%
AGTGCCTACAACTTGGGAAC+TGG - chr8.4:340868-340887 MS.gene43469:CDS 50.0%
ATGGTGAAAGCGGAGGTAGT+TGG + chr8.4:340005-340024 None:intergenic 50.0%
CATCTGCCAGGTCCATTACA+TGG + chr8.4:340715-340734 None:intergenic 50.0%
CGGAGGTAGTTGGAAAGTTG+AGG + chr8.4:339995-340014 None:intergenic 50.0%
GAGATTGGCGAAGATCCAAG+GGG - chr8.4:340488-340507 MS.gene43469:intron 50.0%
GAGTGGGCAATACCAATCGT+CGG - chr8.4:339817-339836 MS.gene43469:CDS 50.0%
GGAGATCGACATGGTGAAAG+CGG + chr8.4:340015-340034 None:intergenic 50.0%
GGCAATACCAATCGTCGGAA+AGG - chr8.4:339822-339841 MS.gene43469:CDS 50.0%
GGGGTTCATTGGATCACACA+CGG - chr8.4:339871-339890 MS.gene43469:CDS 50.0%
GTGAGATTGGCGAAGATCCA+AGG - chr8.4:340486-340505 MS.gene43469:intron 50.0%
GTGGATCACAGCGTCAAACT+TGG + chr8.4:340074-340093 None:intergenic 50.0%
TACCAGCTTGAGCTTGGGTT+TGG - chr8.4:341320-341339 MS.gene43469:CDS 50.0%
TCCAAGAAGGAGCCAAACGA+AGG + chr8.4:341022-341041 None:intergenic 50.0%
TCTCCGCAACAAGCAAGAGT+TGG - chr8.4:340030-340049 MS.gene43469:CDS 50.0%
TGGGCAAGCAACAATCCATG+GGG - chr8.4:341296-341315 MS.gene43469:CDS 50.0%
TTCAAAAGGCAGAGCCAGAG+TGG - chr8.4:340414-340433 MS.gene43469:CDS 50.0%
TTGCCCGAGCTCAACGTTTA+TGG - chr8.4:340560-340579 MS.gene43469:CDS 50.0%
! AAGGATTCATGGCTTGCAGG+TGG + chr8.4:340279-340298 None:intergenic 50.0%
! TCCTTCGTTTGGCTCCTTCT+TGG - chr8.4:341018-341037 MS.gene43469:CDS 50.0%
! TTTGGTGGTCTGAAAGCCGT+GGG - chr8.4:340094-340113 MS.gene43469:intron 50.0%
!! CATGTAATGGACCTGGCAGA+TGG - chr8.4:340713-340732 MS.gene43469:CDS 50.0%
AAAAGGCAGAGCCAGAGTGG+AGG - chr8.4:340417-340436 MS.gene43469:CDS 55.0%
AAAATGTGTCCGAGGAGGCC+CGG - chr8.4:341066-341085 MS.gene43469:CDS 55.0%
ACCGCAGTGCCATCTTTGGT+GGG + chr8.4:340596-340615 None:intergenic 55.0%
ACTCACCGCAGTGCCATCTT+TGG + chr8.4:340600-340619 None:intergenic 55.0%
AGCTCAAGCTGGTATCCCCA+TGG + chr8.4:341314-341333 None:intergenic 55.0%
AGGTGGAAGTCCTCAACGCA+GGG + chr8.4:340262-340281 None:intergenic 55.0%
CAACCAAATGGCGAACCCTG+TGG + chr8.4:339970-339989 None:intergenic 55.0%
CACAGGGTTCGCCATTTGGT+TGG - chr8.4:339968-339987 MS.gene43469:intron 55.0%
CAGCAATGTGACCATCTGCC+AGG + chr8.4:340727-340746 None:intergenic 55.0%
CCTGTGGACAGCTTCCATGA+CGG + chr8.4:339954-339973 None:intergenic 55.0%
CGGCTTTCAGACCACCAAAG+TGG + chr8.4:340093-340112 None:intergenic 55.0%
CGTCATGGAAGCTGTCCACA+GGG - chr8.4:339952-339971 MS.gene43469:intron 55.0%
CTCTGCGACACTTTCTCCCA+CGG + chr8.4:340113-340132 None:intergenic 55.0%
GACGCTGTGATCCACTTTGG+TGG - chr8.4:340079-340098 MS.gene43469:CDS 55.0%
GATCGACATGGTGAAAGCGG+AGG + chr8.4:340012-340031 None:intergenic 55.0%
GCACATGAGAGCGGTGAGAT+TGG - chr8.4:340473-340492 MS.gene43469:intron 55.0%
GGGGATACCAGCTTGAGCTT+GGG - chr8.4:341315-341334 MS.gene43469:CDS 55.0%
TACAACTTGGGAACTGGCCG+CGG - chr8.4:340874-340893 MS.gene43469:CDS 55.0%
TCATTGGGAGGAAGGGAGGA+GGG - chr8.4:341165-341184 MS.gene43469:CDS 55.0%
TCCCAAACCCAAGCTCAAGC+TGG + chr8.4:341325-341344 None:intergenic 55.0%
TCCCACCAAAGATGGCACTG+CGG - chr8.4:340592-340611 MS.gene43469:CDS 55.0%
TGGGGATACCAGCTTGAGCT+TGG - chr8.4:341314-341333 MS.gene43469:CDS 55.0%
TGTCCACAGGGTTCGCCATT+TGG - chr8.4:339964-339983 MS.gene43469:intron 55.0%
TTCATTGGGAGGAAGGGAGG+AGG - chr8.4:341164-341183 MS.gene43469:CDS 55.0%
! CTTTGGTGGTCTGAAAGCCG+TGG - chr8.4:340093-340112 MS.gene43469:intron 55.0%
! GCTTGTTGCGGAGATCGACA+TGG + chr8.4:340024-340043 None:intergenic 55.0%
!! ATAATGGTAACGGGTGGGGC+GGG - chr8.4:339851-339870 MS.gene43469:CDS 55.0%
!! GATAATGGTAACGGGTGGGG+CGG - chr8.4:339850-339869 MS.gene43469:CDS 55.0%
CACCGCAGTGCCATCTTTGG+TGG + chr8.4:340597-340616 None:intergenic 60.0%
CAGGTGGAAGTCCTCAACGC+AGG + chr8.4:340263-340282 None:intergenic 60.0%
CCGTCATGGAAGCTGTCCAC+AGG - chr8.4:339951-339970 MS.gene43469:intron 60.0%
GCGGCCAGTTCCCAAGTTGT+AGG + chr8.4:340875-340894 None:intergenic 60.0%
!! TAATGGTAACGGGTGGGGCG+GGG - chr8.4:339852-339871 MS.gene43469:CDS 60.0%
CCGCTCTCATGTGCCCCAAC+CGG + chr8.4:340467-340486 None:intergenic 65.0%
CCGGTTGGGGCACATGAGAG+CGG - chr8.4:340464-340483 MS.gene43469:intron 65.0%
GTAGCATCTCCGGGCCTCCT+CGG + chr8.4:341078-341097 None:intergenic 65.0%
ACGGGTGGGGCGGGGTTCAT+TGG - chr8.4:339860-339879 MS.gene43469:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 339812 341352 339812 ID=MS.gene43469
chr8.4 mRNA 339812 341352 339812 ID=MS.gene43469.t1;Parent=MS.gene43469
chr8.4 exon 340852 341352 340852 ID=MS.gene43469.t1.exon1;Parent=MS.gene43469.t1
chr8.4 CDS 340852 341352 340852 ID=cds.MS.gene43469.t1;Parent=MS.gene43469.t1
chr8.4 exon 340551 340778 340551 ID=MS.gene43469.t1.exon2;Parent=MS.gene43469.t1
chr8.4 CDS 340551 340778 340551 ID=cds.MS.gene43469.t1;Parent=MS.gene43469.t1
chr8.4 exon 340385 340466 340385 ID=MS.gene43469.t1.exon3;Parent=MS.gene43469.t1
chr8.4 CDS 340385 340466 340385 ID=cds.MS.gene43469.t1;Parent=MS.gene43469.t1
chr8.4 exon 340219 340301 340219 ID=MS.gene43469.t1.exon4;Parent=MS.gene43469.t1
chr8.4 CDS 340219 340301 340219 ID=cds.MS.gene43469.t1;Parent=MS.gene43469.t1
chr8.4 exon 340019 340110 340019 ID=MS.gene43469.t1.exon5;Parent=MS.gene43469.t1
chr8.4 CDS 340019 340110 340019 ID=cds.MS.gene43469.t1;Parent=MS.gene43469.t1
chr8.4 exon 339812 339911 339812 ID=MS.gene43469.t1.exon6;Parent=MS.gene43469.t1
chr8.4 CDS 339812 339911 339812 ID=cds.MS.gene43469.t1;Parent=MS.gene43469.t1
Gene Sequence

>MS.gene43469

ATGGTGAGTGGGCAATACCAATCGTCGGAAAGGAAAACGATAATGGTAACGGGTGGGGCGGGGTTCATTGGATCACACACGGTCGTTCAGCTTCTAAAACAGGGCTATACAGTTTCCATTATCGACAATCTCTACAATTCCGTCATGGAAGCTGTCCACAGGGTTCGCCATTTGGTTGGTCCTCAACTTTCCAACTACCTCCGCTTTCACCATGTCGATCTCCGCAACAAGCAAGAGTTGGAGATGATCTTCTCTAAAACCAAGTTTGACGCTGTGATCCACTTTGGTGGTCTGAAAGCCGTGGGAGAAAGTGTCGCAGAGCCACTCAAATATTTTGATCACAACTTGGTTGGCACTACCAATCTTTTTCAAGTGATGACTAAATTTCAGTGTAAAAAGATGGTATTGTCATCCTCTGCAGCAGTTTATGGTCAACCAGATGAAATTCCCTGCGTTGAGGACTTCCACCTGCAAGCCATGAATCCTTATGGAAGAACCAAGGTACTTAACTCAGTATATATCCATTAAAATAATGAATAATGATACTTAAGCTGGCATATATGATATATGACAGCTTTTCGCGGAAGAGATTGCACGAGATATTCAAAAGGCAGAGCCAGAGTGGAGGATAATATTGCTGAGATACTTCAATCCGGTTGGGGCACATGAGAGCGGTGAGATTGGCGAAGATCCAAGGGGCATACCCAATAATCTGATGCCTTACTTAACCCAAGTAGCAGTTGGAAGATTGCCCGAGCTCAACGTTTATGGTCATGATTATCCCACCAAAGATGGCACTGCGGTGAGTGTCTTAATTCACTCATCATATTGATATTTACCAGTTAGCACCCAACATATATTCTGATACTGACAATATATGAATCAGATACGGGACTTTATCCATGTAATGGACCTGGCAGATGGTCACATTGCTGCGTTGCGAAAGCTTTTTGCGACAGACAAAATCGGTAGGTACCTACTTACATTTAACTATTTATTTGAATTGTAGATTAGAGCTAACCAGGACTTAATTAAATACTGTTATTATTAGGTTGTAGTGCCTACAACTTGGGAACTGGCCGCGGTACATCAGTGCTTCAAATGGTTGCTGCATTTGAAAAGGCTTCTGGCAAGGTCTTTTTTTTTATATAGTCTTCTGTACTGTAGCATGTATGCATCTCCGAATGTATTGTATTGTATTCTTGATCCTTCGTTTGGCTCCTTCTTGGATCTGTTTTCCAGAAAATTCCTATTAAAATGTGTCCGAGGAGGCCCGGAGATGCTACTGCTATCTATGCATCTACGCATAAGGCTGAAAATGAACTTGGTTGGAAGTAAGTACTTTACTATATTTCATTGGGAGGAAGGGAGGAGGGATTATTACATTCTTTTAATTAACTAATAATAAAAAGAGTGTGCTTTATTTACGATTGATTGACAGGGCAAAATACGGTATAGAGGAGATGTGCAGAGACCAATGGAATTGGGCAAGCAACAATCCATGGGGATACCAGCTTGAGCTTGGGTTTGGGAATCGTTGA
Protein sequence

>MS.gene43469.t1

MVSGQYQSSERKTIMVTGGAGFIGSHTVVQLLKQGYTVSIIDNLYNSVMEAVHRVRHLVGPQLSNYLRFHHVDLRNKQELEMIFSKTKFDAVIHFGGLKAVGESVAEPLKYFDHNLVGTTNLFQVMTKFQCKKMVLSSSAAVYGQPDEIPCVEDFHLQAMNPYGRTKLFAEEIARDIQKAEPEWRIILLRYFNPVGAHESGEIGEDPRGIPNNLMPYLTQVAVGRLPELNVYGHDYPTKDGTAIRDFIHVMDLADGHIAALRKLFATDKIGCSAYNLGTGRGTSVLQMVAAFEKASGKKIPIKMCPRRPGDATAIYASTHKAENELGWKAKYGIEEMCRDQWNWASNNPWGYQLELGFGNR