AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43479


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43479.t1 MTR_4g134630 95.033 302 10 1 1 297 1 302 0.0 582
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43479.t1 AT2G02570 66.667 297 85 3 12 297 6 299 1.85e-143 405
MS.gene43479.t1 AT2G02570 66.667 297 85 3 12 297 6 299 1.85e-143 405
MS.gene43479.t1 AT2G02570 66.667 297 85 3 12 297 6 299 1.85e-143 405
MS.gene43479.t1 AT2G02570 63.300 297 83 4 12 297 6 287 9.88e-133 378

Find 78 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCGCATCTTAGACTTAA+AGG 0.209233 8.4:-241940 None:intergenic
TGTGGGTGGGTGTTGGAGAT+TGG 0.224072 8.4:-240206 None:intergenic
GTGGGTGGGTGTTGGAGATT+GGG 0.293108 8.4:-240205 None:intergenic
GGCTCTATACGAAGCTTTGC+AGG 0.323302 8.4:-241634 None:intergenic
TCCTGTTGGCACCAGAGTTC+AGG 0.328664 8.4:+240359 MS.gene43479:CDS
TCAACAGTTCCTTCTTGAAT+TGG 0.334241 8.4:-241511 None:intergenic
CTTCAAGATTGGTTTCTTCT+CGG 0.338340 8.4:+242643 MS.gene43479:intron
AGAAGGAACTGTTGATCCTT+TGG 0.344531 8.4:+241519 MS.gene43479:CDS
TGTTGGAGATTGGGTGGAAG+TGG 0.361676 8.4:-240196 None:intergenic
CAAGGAAAGGTTGGTGTGAC+TGG 0.362128 8.4:+242707 MS.gene43479:CDS
TTACAAACAACAACTTCATC+AGG 0.389377 8.4:+239550 MS.gene43479:CDS
CAACAGGAAATTTGTGTTGA+TGG 0.395602 8.4:-240344 None:intergenic
ACCTTTCCTTGAGGATCATC+AGG 0.403855 8.4:-242695 None:intergenic
AACACCCACCCACAATAAAC+TGG 0.407152 8.4:+240215 MS.gene43479:CDS
AAGGTTGGTGTGACTGGAAG+TGG 0.416029 8.4:+242713 MS.gene43479:CDS
TTGAATTGGCCTTATGTTAG+CGG 0.420910 8.4:-241497 None:intergenic
CAGGCTGTTTATAGTGATGA+TGG 0.431282 8.4:+240378 MS.gene43479:CDS
GCCTGAACTCTGGTGCCAAC+AGG 0.438372 8.4:-240360 None:intergenic
TTCAAGATTGGTTTCTTCTC+GGG 0.438391 8.4:+242644 MS.gene43479:intron
AGTTTATTGTGGGTGGGTGT+TGG 0.447717 8.4:-240213 None:intergenic
ACACAACTCACCACATCCTC+AGG 0.452174 8.4:-241664 None:intergenic
ACCACATCCTCAGGGTCATC+GGG 0.452351 8.4:-241655 None:intergenic
GTTGGAGATTGGGTGGAAGT+GGG 0.456301 8.4:-240195 None:intergenic
AAGAAGATGCAAGGTGGTAG+CGG 0.461653 8.4:+239470 None:intergenic
TTGTTTGTAAGTAGAAAGAT+TGG 0.462862 8.4:-239537 None:intergenic
TACAATCGAGAATGATGATT+AGG 0.470705 8.4:+242790 MS.gene43479:CDS
TAGCTGACATAATAACCATT+TGG 0.474920 8.4:-240435 None:intergenic
CACCACATCCTCAGGGTCAT+CGG 0.475099 8.4:-241656 None:intergenic
GGTGTGACTGGAAGTGGGAA+AGG 0.475251 8.4:+242719 MS.gene43479:CDS
AGGTAGATCCCGCTAACATA+AGG 0.476232 8.4:+241488 MS.gene43479:intron
TCGACATACTCAGAATTAGA+TGG 0.482981 8.4:-239692 None:intergenic
TGAATTGGCCTTATGTTAGC+GGG 0.489549 8.4:-241496 None:intergenic
ATTATGTCAGCTATGACACT+TGG 0.510217 8.4:+240445 MS.gene43479:CDS
GTCGAGGCTTACACACCAAA+TGG 0.519193 8.4:+240420 MS.gene43479:CDS
AAAATAAGCGGCAAAATGCT+TGG 0.521225 8.4:+241982 MS.gene43479:CDS
AGGCTGTTTATAGTGATGAT+GGG 0.524754 8.4:+240379 MS.gene43479:CDS
CGTACATACCAGTTTATTGT+GGG 0.530390 8.4:-240223 None:intergenic
AGAAGATGCAAGGTGGTAGC+GGG 0.534654 8.4:+239471 None:intergenic
TTATGTCAGCTATGACACTT+GGG 0.535353 8.4:+240446 MS.gene43479:CDS
ACATACCAGTTTATTGTGGG+TGG 0.536199 8.4:-240220 None:intergenic
AGTTGCTGAAGCTACAAAGC+AGG 0.562918 8.4:+241555 MS.gene43479:CDS
TCCTGATGATCCTCAAGGAA+AGG 0.563297 8.4:+242694 MS.gene43479:CDS
CTACAAAGCAGGCTATCAAA+CGG 0.564191 8.4:+241566 MS.gene43479:CDS
GGTGGGTGTTGGAGATTGGG+TGG 0.565121 8.4:-240202 None:intergenic
ACGTACATACCAGTTTATTG+TGG 0.565424 8.4:-240224 None:intergenic
ACTATAAACAGCCTGAACTC+TGG 0.565916 8.4:-240370 None:intergenic
ATAAAGAGAAAGAAGATGCA+AGG 0.565982 8.4:+239461 None:intergenic
AAATCTCCTGATGATCCTCA+AGG 0.567592 8.4:+242689 MS.gene43479:CDS
GCAAGGTGGTAGCGGGCGTG+AGG 0.569536 8.4:+239478 MS.gene43479:CDS
CAAACGGAAAATTGCGCAAG+CGG 0.570501 8.4:+241582 MS.gene43479:CDS
CATCAACACAAATTTCCTGT+TGG 0.571494 8.4:+240345 MS.gene43479:CDS
GATGATCCTCAAGGAAAGGT+TGG 0.575071 8.4:+242698 MS.gene43479:CDS
GCCCGATGACCCTGAGGATG+TGG 0.576654 8.4:+241654 MS.gene43479:CDS
ATTTGTGTTGATGGTCAAAG+TGG 0.580757 8.4:-240335 None:intergenic
AACATAAGGCCAATTCAAGA+AGG 0.580826 8.4:+241502 MS.gene43479:CDS
ATTTGGTGTGTAAGCCTCGA+CGG 0.580845 8.4:-240418 None:intergenic
TTTATAGTGATGATGGGGAG+TGG 0.581469 8.4:+240385 MS.gene43479:CDS
GGCTGTTTATAGTGATGATG+GGG 0.588465 8.4:+240380 MS.gene43479:CDS
AGGTTGGTGTGACTGGAAGT+GGG 0.590449 8.4:+242714 MS.gene43479:CDS
CATACCAGTTTATTGTGGGT+GGG 0.590879 8.4:-240219 None:intergenic
CAGCAGTTCCAGACAACCAA+GGG 0.593266 8.4:+242005 MS.gene43479:CDS
GATGGTCAAAGTGGGTATGA+AGG 0.597115 8.4:-240326 None:intergenic
ACTCGCTCAGCTTCAACCAA+AGG 0.606860 8.4:-241535 None:intergenic
AGATTGGTTTCTTCTCGGGA+AGG 0.610846 8.4:+242648 MS.gene43479:intron
AAGAGAAAGAAGATGCAAGG+TGG 0.627035 8.4:+239464 None:intergenic
TTTGTGTTGATGGTCAAAGT+GGG 0.628380 8.4:-240334 None:intergenic
CACTTGGGGTAATAAAGAAG+AGG 0.630391 8.4:+240461 MS.gene43479:CDS
TTGAAGTCACCCAAAATAAG+CGG 0.630424 8.4:+241970 MS.gene43479:CDS
GCAGCAGTTCCAGACAACCA+AGG 0.644897 8.4:+242004 MS.gene43479:CDS
CACAACTCACCACATCCTCA+GGG 0.646183 8.4:-241663 None:intergenic
TAATTCTGAGTATGTCGACA+TGG 0.651465 8.4:+239697 MS.gene43479:CDS
CTTTAAGTCTAAGATGCGGA+TGG 0.652129 8.4:+241941 MS.gene43479:CDS
GTCACACCAACCTTTCCTTG+AGG 0.671537 8.4:-242704 None:intergenic
GTGGTATGATGCAACCGTCG+AGG 0.674698 8.4:+240404 MS.gene43479:CDS
TATGTCAGCTATGACACTTG+GGG 0.685361 8.4:+240447 MS.gene43479:CDS
ATGCCTTTAAGTCTAAGATG+CGG 0.694744 8.4:+241937 MS.gene43479:CDS
TATAGAGCCCGATGACCCTG+AGG 0.709309 8.4:+241648 MS.gene43479:CDS
CATGGAAAGAGAGCTCTCCG+AGG 0.756940 8.4:+239715 MS.gene43479:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTGAAAAAAGAATATA+AGG + chr8.4:242501-242520 MS.gene43479:intron 10.0%
!!! AATAAAAATGAATATTTTGT+AGG + chr8.4:241373-241392 MS.gene43479:intron 10.0%
!!! ATAAAAATGAATATTTTGTA+GGG + chr8.4:241374-241393 MS.gene43479:intron 10.0%
!!! TTTTCTTGTTAATTAATAAT+GGG - chr8.4:239859-239878 None:intergenic 10.0%
!! AAATAGATAAATAATGTTGT+TGG - chr8.4:240021-240040 None:intergenic 15.0%
!!! AAACTCATTATAAAATTTTC+TGG + chr8.4:240831-240850 MS.gene43479:intron 15.0%
!!! AACAATAAAAAGTATTTTAC+TGG + chr8.4:241082-241101 MS.gene43479:intron 15.0%
!!! GTTGTTATTATTAATTGAAT+GGG - chr8.4:239837-239856 None:intergenic 15.0%
!!! GTTTTCTTGTTAATTAATAA+TGG - chr8.4:239860-239879 None:intergenic 15.0%
!!! TGGAGTATTTTTTTTTTATA+GGG - chr8.4:241415-241434 None:intergenic 15.0%
!!! TTTGTTTGTTTGTTTTTTTA+GGG + chr8.4:239577-239596 MS.gene43479:intron 15.0%
!! AACATGGAAAGAAAAAAAAA+AGG - chr8.4:240596-240615 None:intergenic 20.0%
!! AGAACACTTGTTTATATAAA+AGG - chr8.4:241280-241299 None:intergenic 20.0%
!! ATAAAAAAAAAATACTCCAG+TGG + chr8.4:241416-241435 MS.gene43479:intron 20.0%
!! ATATAAAACACAAATATCAG+TGG - chr8.4:242551-242570 None:intergenic 20.0%
!! TAATGCAATGGTAAATAAAT+TGG - chr8.4:240562-240581 None:intergenic 20.0%
!! TAATTAATATTAGTGAGAGT+TGG - chr8.4:240070-240089 None:intergenic 20.0%
!! TATAATGCTGAAGAAAAAAA+CGG - chr8.4:240760-240779 None:intergenic 20.0%
!!! ACCTGTTTTTATAAAATGTA+GGG + chr8.4:241326-241345 MS.gene43479:intron 20.0%
!!! AGCTAAAAAATATTTTCACA+TGG - chr8.4:242386-242405 None:intergenic 20.0%
!!! ATTAATGTTTGCTATTGAAA+GGG + chr8.4:241168-241187 MS.gene43479:intron 20.0%
!!! CTGGAGTATTTTTTTTTTAT+AGG - chr8.4:241416-241435 None:intergenic 20.0%
!!! GGTTGTTATTATTAATTGAA+TGG - chr8.4:239838-239857 None:intergenic 20.0%
!!! GTCTTAATAAATATTTTCCT+TGG + chr8.4:241732-241751 MS.gene43479:intron 20.0%
!!! GTTTGTTTGTTTGTTTTTTT+AGG + chr8.4:239576-239595 MS.gene43479:intron 20.0%
!!! TACCTGTTTTTATAAAATGT+AGG + chr8.4:241325-241344 MS.gene43479:intron 20.0%
! AAAAGGTAGGAAACTAAAAA+GGG - chr8.4:241263-241282 None:intergenic 25.0%
! AATAGTAGAGAAATTGAGAA+AGG - chr8.4:242047-242066 None:intergenic 25.0%
! AGAAAAGAAAAGAAAAACCT+CGG - chr8.4:239735-239754 None:intergenic 25.0%
! AGACAGATTCATAATTCTAT+AGG - chr8.4:241212-241231 None:intergenic 25.0%
! AGGGTCCAAAAAATATATAT+AGG - chr8.4:241244-241263 None:intergenic 25.0%
! CACTTGTTTATATAAAAGGT+AGG - chr8.4:241276-241295 None:intergenic 25.0%
! GACAGATTCATAATTCTATA+GGG - chr8.4:241211-241230 None:intergenic 25.0%
! GCTTCAAATTGAAATTGAAA+AGG + chr8.4:239794-239813 MS.gene43479:intron 25.0%
! GGGTCCAAAAAATATATATA+GGG - chr8.4:241243-241262 None:intergenic 25.0%
! TAAAAGGTAGGAAACTAAAA+AGG - chr8.4:241264-241283 None:intergenic 25.0%
! TATGTCTTAAAGTGACTAAT+GGG + chr8.4:242143-242162 MS.gene43479:intron 25.0%
! TGATGAGTAATGAATAGATT+AGG - chr8.4:240275-240294 None:intergenic 25.0%
! TTAGTTGAGAAAAGAAATAG+AGG + chr8.4:240944-240963 MS.gene43479:intron 25.0%
! TTCTCAACTAAACAAAAAAC+TGG - chr8.4:240935-240954 None:intergenic 25.0%
! TTGTTTGTAAGTAGAAAGAT+TGG - chr8.4:239540-239559 None:intergenic 25.0%
!! ATAAATTCAACAACTTTTCC+AGG - chr8.4:242486-242505 None:intergenic 25.0%
!! ATTGTGAAAATTCTAGAGAT+CGG - chr8.4:242528-242547 None:intergenic 25.0%
!! TAAATTCAACAACTTTTCCA+GGG - chr8.4:242485-242504 None:intergenic 25.0%
!! TTTTCTGCAAATGTATTTCT+TGG + chr8.4:241798-241817 MS.gene43479:intron 25.0%
!!! CATTAATGTTTGCTATTGAA+AGG + chr8.4:241167-241186 MS.gene43479:intron 25.0%
!!! CCTGTTTTTATAAAATGTAG+GGG + chr8.4:241327-241346 MS.gene43479:intron 25.0%
!!! CTATGTTTTTCACTGTTTTT+AGG + chr8.4:240302-240321 MS.gene43479:intron 25.0%
!!! TCGTCCCTATATATATTTTT+TGG + chr8.4:241236-241255 MS.gene43479:intron 25.0%
!!! TGTTTGTTTTTTTAGGGTTA+GGG + chr8.4:239583-239602 MS.gene43479:intron 25.0%
!!! TTGTTTGTTTTTTTAGGGTT+AGG + chr8.4:239582-239601 MS.gene43479:intron 25.0%
AAAATCAGTAACTAAACCAC+TGG - chr8.4:241435-241454 None:intergenic 30.0%
AATCTATTTACCCCTTGAAT+CGG + chr8.4:242416-242435 MS.gene43479:intron 30.0%
ACAATACAGATGCAATAAGT+TGG - chr8.4:240725-240744 None:intergenic 30.0%
AGAAAACTGGCAGATAAATT+TGG - chr8.4:241853-241872 None:intergenic 30.0%
AGGGGCTAAAAATTGTTTAT+AGG + chr8.4:241345-241364 MS.gene43479:intron 30.0%
ATTTAAGGAGACATTGGAAT+TGG - chr8.4:240111-240130 None:intergenic 30.0%
GTATGTCTTAAAGTGACTAA+TGG + chr8.4:242142-242161 MS.gene43479:intron 30.0%
TAGCTGACATAATAACCATT+TGG - chr8.4:240438-240457 None:intergenic 30.0%
TAGTTTCTATGCAAGAATGT+AGG + chr8.4:242109-242128 MS.gene43479:intron 30.0%
TATCATTGCAGTCATTAAGT+TGG + chr8.4:242304-242323 MS.gene43479:intron 30.0%
TATGTTAGGATGGTCAAAAA+TGG - chr8.4:242184-242203 None:intergenic 30.0%
TGAGTATCAAATCATGTAAC+AGG + chr8.4:240864-240883 MS.gene43479:intron 30.0%
TGCAATGGTAAATAAATTGG+AGG - chr8.4:240559-240578 None:intergenic 30.0%
TGTAGAATTTAAGGAGACAT+TGG - chr8.4:240117-240136 None:intergenic 30.0%
TGTTGTATGTATCTATCTCA+TGG - chr8.4:239970-239989 None:intergenic 30.0%
TTACAAACAACAACTTCATC+AGG + chr8.4:239550-239569 MS.gene43479:CDS 30.0%
TTGTTCTTTCCATTTCAACA+AGG - chr8.4:241067-241086 None:intergenic 30.0%
! AAGTTCTATGTGATTTTTGC+TGG + chr8.4:240649-240668 MS.gene43479:intron 30.0%
! AATTTATCTGCCAGTTTTCT+AGG + chr8.4:241853-241872 MS.gene43479:intron 30.0%
! AGTTAGCAAACATGTCATTT+AGG - chr8.4:241883-241902 None:intergenic 30.0%
! ATAAGTTGGAAATGCAATTG+TGG - chr8.4:240711-240730 None:intergenic 30.0%
! ATATGTTGCTTGCTTATTAC+AGG + chr8.4:241468-241487 MS.gene43479:intron 30.0%
! CCCCTACATTTTATAAAAAC+AGG - chr8.4:241330-241349 None:intergenic 30.0%
! GTTAGCAAACATGTCATTTA+GGG - chr8.4:241882-241901 None:intergenic 30.0%
! TATTTCATTTTGCTTAGCAG+TGG - chr8.4:240175-240194 None:intergenic 30.0%
! TTGTGAAAATTCTAGAGATC+GGG - chr8.4:242527-242546 None:intergenic 30.0%
!!! AGAAAAGCATTTTCATCTGA+AGG + chr8.4:242763-242782 MS.gene43479:CDS 30.0%
!!! GGTTTAGTTACTGATTTTCA+AGG + chr8.4:241437-241456 MS.gene43479:intron 30.0%
!!! TTTTTTTAGGGTTAGGGTTA+GGG + chr8.4:239589-239608 MS.gene43479:intron 30.0%
AAAAGTAATACCATCAACCC+TGG + chr8.4:242465-242484 MS.gene43479:intron 35.0%
AAAATAAGCGGCAAAATGCT+TGG + chr8.4:241982-242001 MS.gene43479:CDS 35.0%
AAACGCTTCAATAAACGTGA+TGG - chr8.4:240911-240930 None:intergenic 35.0%
AACATAAGGCCAATTCAAGA+AGG + chr8.4:241502-241521 MS.gene43479:CDS 35.0%
AACCAAGGGAAAAGCAAAAA+AGG + chr8.4:242019-242038 MS.gene43479:CDS 35.0%
AACGCTTCAATAAACGTGAT+GGG - chr8.4:240910-240929 None:intergenic 35.0%
AATGTATGCAGATGTAATGC+AGG - chr8.4:240534-240553 None:intergenic 35.0%
ACGTACATACCAGTTTATTG+TGG - chr8.4:240227-240246 None:intergenic 35.0%
AGAGGAATCACTTTATCCTA+GGG + chr8.4:240962-240981 MS.gene43479:intron 35.0%
AGCAAGATAGATAGAAGTGA+TGG - chr8.4:239915-239934 None:intergenic 35.0%
AGGAATGAGATTTCTCAACT+TGG - chr8.4:240998-241017 None:intergenic 35.0%
AGGCTGTTTATAGTGATGAT+GGG + chr8.4:240379-240398 MS.gene43479:CDS 35.0%
ATGTATGCAGATGTAATGCA+GGG - chr8.4:240533-240552 None:intergenic 35.0%
ATTATGTCAGCTATGACACT+TGG + chr8.4:240445-240464 MS.gene43479:CDS 35.0%
CAACAGGAAATTTGTGTTGA+TGG - chr8.4:240347-240366 None:intergenic 35.0%
CATCAACACAAATTTCCTGT+TGG + chr8.4:240345-240364 MS.gene43479:CDS 35.0%
CCAAATATATCATGCACTCA+TGG - chr8.4:240798-240817 None:intergenic 35.0%
CCATGAGTGCATGATATATT+TGG + chr8.4:240795-240814 MS.gene43479:intron 35.0%
CGTACATACCAGTTTATTGT+GGG - chr8.4:240226-240245 None:intergenic 35.0%
GCAAATGTATTTCTTGGTAG+TGG + chr8.4:241804-241823 MS.gene43479:intron 35.0%
GCAAGATAGATAGAAGTGAT+GGG - chr8.4:239914-239933 None:intergenic 35.0%
GTACGACATAGATAATGCAA+TGG - chr8.4:240574-240593 None:intergenic 35.0%
TAATTCTGAGTATGTCGACA+TGG + chr8.4:239697-239716 MS.gene43479:CDS 35.0%
TAGAACAAGAGATCAAACCA+AGG - chr8.4:241752-241771 None:intergenic 35.0%
TAGAGGAATCACTTTATCCT+AGG + chr8.4:240961-240980 MS.gene43479:intron 35.0%
TCAACAGTTCCTTCTTGAAT+TGG - chr8.4:241514-241533 None:intergenic 35.0%
TCCTTAAATTCTACAACACC+AGG + chr8.4:240122-240141 MS.gene43479:intron 35.0%
TCGACATACTCAGAATTAGA+TGG - chr8.4:239695-239714 None:intergenic 35.0%
TCTCTAACCTGAAAAGAGAA+GGG - chr8.4:239656-239675 None:intergenic 35.0%
TGTATGTATCTATCTCATGG+CGG - chr8.4:239967-239986 None:intergenic 35.0%
TTATGTCAGCTATGACACTT+GGG + chr8.4:240446-240465 MS.gene43479:CDS 35.0%
TTCTCTAACCTGAAAAGAGA+AGG - chr8.4:239657-239676 None:intergenic 35.0%
TTGAAGTCACCCAAAATAAG+CGG + chr8.4:241970-241989 MS.gene43479:CDS 35.0%
TTTAGGGAATCCTAGAAAAC+TGG - chr8.4:241866-241885 None:intergenic 35.0%
! AAGCATTTTCATCTGAAGGA+TGG + chr8.4:242767-242786 MS.gene43479:CDS 35.0%
! ATGCCTTTAAGTCTAAGATG+CGG + chr8.4:241937-241956 MS.gene43479:CDS 35.0%
! CTTTTGTGATGTATTCACTG+TGG + chr8.4:242615-242634 MS.gene43479:intron 35.0%
! GAAAATGCTTTTCTCTTCTC+TGG - chr8.4:242757-242776 None:intergenic 35.0%
! GGCATGTATTTTCTTACGTT+TGG - chr8.4:241922-241941 None:intergenic 35.0%
! TTGTAAGTTGGATAGGAATG+AGG + chr8.4:242439-242458 MS.gene43479:intron 35.0%
! TTTGATTCCCTTCTCTTTTC+AGG + chr8.4:239646-239665 MS.gene43479:intron 35.0%
!! AAGCATTTTGCCGCTTATTT+TGG - chr8.4:241983-242002 None:intergenic 35.0%
!! ACCTGGTGTTGTAGAATTTA+AGG - chr8.4:240126-240145 None:intergenic 35.0%
!! AGCATTTTGCCGCTTATTTT+GGG - chr8.4:241982-242001 None:intergenic 35.0%
!! ATTTGTGTTGATGGTCAAAG+TGG - chr8.4:240338-240357 None:intergenic 35.0%
!! CTTCAAGATTGGTTTCTTCT+CGG + chr8.4:242643-242662 MS.gene43479:intron 35.0%
!! TTCAAGATTGGTTTCTTCTC+GGG + chr8.4:242644-242663 MS.gene43479:intron 35.0%
!! TTGAATTGGCCTTATGTTAG+CGG - chr8.4:241500-241519 None:intergenic 35.0%
!! TTTGTGTTGATGGTCAAAGT+GGG - chr8.4:240337-240356 None:intergenic 35.0%
!!! CATCTGTTGATTTTCAGTCA+CGG + chr8.4:241605-241624 MS.gene43479:CDS 35.0%
!!! GTTTTTTTAGGGTTAGGGTT+AGG + chr8.4:239588-239607 MS.gene43479:intron 35.0%
!!! TACCTTTTTTGCTTTTCCCT+TGG - chr8.4:242024-242043 None:intergenic 35.0%
AAATCTCCTGATGATCCTCA+AGG + chr8.4:242689-242708 MS.gene43479:CDS 40.0%
ACATACCAGTTTATTGTGGG+TGG - chr8.4:240223-240242 None:intergenic 40.0%
ACTATAAACAGCCTGAACTC+TGG - chr8.4:240373-240392 None:intergenic 40.0%
AGAAGGAACTGTTGATCCTT+TGG + chr8.4:241519-241538 MS.gene43479:CDS 40.0%
CACTTGGGGTAATAAAGAAG+AGG + chr8.4:240461-240480 MS.gene43479:CDS 40.0%
CAGGCTGTTTATAGTGATGA+TGG + chr8.4:240378-240397 MS.gene43479:CDS 40.0%
CATACCAGTTTATTGTGGGT+GGG - chr8.4:240222-240241 None:intergenic 40.0%
CATAGTAGCACACAAAGTAC+AGG - chr8.4:241018-241037 None:intergenic 40.0%
CATCCGCATCTTAGACTTAA+AGG - chr8.4:241943-241962 None:intergenic 40.0%
CCATGTTAGAGAAACAGAGT+TGG + chr8.4:240609-240628 MS.gene43479:intron 40.0%
CTACAAAGCAGGCTATCAAA+CGG + chr8.4:241566-241585 MS.gene43479:CDS 40.0%
GTTAGGATGGTCAAAAATGG+AGG - chr8.4:242181-242200 None:intergenic 40.0%
TATCTATCTTGCTTCTGTGC+AGG + chr8.4:239922-239941 MS.gene43479:intron 40.0%
TATGTCAGCTATGACACTTG+GGG + chr8.4:240447-240466 MS.gene43479:CDS 40.0%
TGTTCTGTACTCCTTAGAGA+GGG + chr8.4:241294-241313 MS.gene43479:intron 40.0%
TTCTAGCCTGAATTTACCAC+AGG + chr8.4:242274-242293 MS.gene43479:intron 40.0%
TTCTCTCCTGTGGTAAATTC+AGG - chr8.4:242283-242302 None:intergenic 40.0%
TTGATGCAATCCTAGTCCTA+TGG + chr8.4:240505-240524 MS.gene43479:intron 40.0%
TTTGCAGCACCTTGTTGAAA+TGG + chr8.4:241055-241074 MS.gene43479:intron 40.0%
! AACAACTTTTCCAGGGTTGA+TGG - chr8.4:242478-242497 None:intergenic 40.0%
! CAACTTACAACCGATTCAAG+GGG - chr8.4:242429-242448 None:intergenic 40.0%
! CCAACTCTGTTTCTCTAACA+TGG - chr8.4:240612-240631 None:intergenic 40.0%
! CCAACTTACAACCGATTCAA+GGG - chr8.4:242430-242449 None:intergenic 40.0%
! CTTTAAGTCTAAGATGCGGA+TGG + chr8.4:241941-241960 MS.gene43479:CDS 40.0%
! GAATCGGTTGTAAGTTGGAT+AGG + chr8.4:242432-242451 MS.gene43479:intron 40.0%
! GGCTGTTTATAGTGATGATG+GGG + chr8.4:240380-240399 MS.gene43479:CDS 40.0%
! GTACTTTTAGTCCCTCTCTA+AGG - chr8.4:241308-241327 None:intergenic 40.0%
! TAAGTTGGATAGGAATGAGG+AGG + chr8.4:242442-242461 MS.gene43479:intron 40.0%
! TCCAACTTACAACCGATTCA+AGG - chr8.4:242431-242450 None:intergenic 40.0%
!! CATTGACTCGTATGTTAGGA+TGG - chr8.4:242194-242213 None:intergenic 40.0%
!! CTGACATTGACTCGTATGTT+AGG - chr8.4:242198-242217 None:intergenic 40.0%
!! CTGTGGTTTTGCTTCAAGAT+TGG + chr8.4:242632-242651 MS.gene43479:intron 40.0%
!! TGAATTGGCCTTATGTTAGC+GGG - chr8.4:241499-241518 None:intergenic 40.0%
!! TTTATAGTGATGATGGGGAG+TGG + chr8.4:240385-240404 MS.gene43479:CDS 40.0%
AACACCCACCCACAATAAAC+TGG + chr8.4:240215-240234 MS.gene43479:CDS 45.0%
ACCTTTCCTTGAGGATCATC+AGG - chr8.4:242698-242717 None:intergenic 45.0%
AGGACTAGGATTGCATCAAG+TGG - chr8.4:240504-240523 None:intergenic 45.0%
AGGTAGATCCCGCTAACATA+AGG + chr8.4:241488-241507 MS.gene43479:intron 45.0%
AGTTGCTGAAGCTACAAAGC+AGG + chr8.4:241555-241574 MS.gene43479:CDS 45.0%
AGTTTATTGTGGGTGGGTGT+TGG - chr8.4:240216-240235 None:intergenic 45.0%
CAAACGGAAAATTGCGCAAG+CGG + chr8.4:241582-241601 MS.gene43479:CDS 45.0%
CATTCTGTTGATGCTGAGTG+AGG - chr8.4:241128-241147 None:intergenic 45.0%
CCCTTGAATCGGTTGTAAGT+TGG + chr8.4:242427-242446 MS.gene43479:intron 45.0%
CTTCAGTCAAAGCAATGACC+TGG - chr8.4:240143-240162 None:intergenic 45.0%
GATGATCCTCAAGGAAAGGT+TGG + chr8.4:242698-242717 MS.gene43479:CDS 45.0%
GATGGTCAAAGTGGGTATGA+AGG - chr8.4:240329-240348 None:intergenic 45.0%
GCAATGATACTTCTCTCCTG+TGG - chr8.4:242293-242312 None:intergenic 45.0%
GGAATCACTTTATCCTAGGG+AGG + chr8.4:240965-240984 MS.gene43479:intron 45.0%
GTGTTCTGTACTCCTTAGAG+AGG + chr8.4:241293-241312 MS.gene43479:intron 45.0%
TAGGGTTAGGGTTAGGGTTA+GGG + chr8.4:239595-239614 MS.gene43479:intron 45.0%
TCCTGATGATCCTCAAGGAA+AGG + chr8.4:242694-242713 MS.gene43479:CDS 45.0%
TTAGGGTTAGGGTTAGGGTT+AGG + chr8.4:239594-239613 MS.gene43479:intron 45.0%
!! AGATTGGTTTCTTCTCGGGA+AGG + chr8.4:242648-242667 MS.gene43479:intron 45.0%
!! ATTTGGTGTGTAAGCCTCGA+CGG - chr8.4:240421-240440 None:intergenic 45.0%
!! TTGCTTTTCCCTTGGTTGTC+TGG - chr8.4:242016-242035 None:intergenic 45.0%
ACACAACTCACCACATCCTC+AGG - chr8.4:241667-241686 None:intergenic 50.0%
ACTCGCTCAGCTTCAACCAA+AGG - chr8.4:241538-241557 None:intergenic 50.0%
ACTTGGAAGTAAGCCTCCCT+AGG - chr8.4:240981-241000 None:intergenic 50.0%
CACAACTCACCACATCCTCA+GGG - chr8.4:241666-241685 None:intergenic 50.0%
CAGCAGTTCCAGACAACCAA+GGG + chr8.4:242005-242024 MS.gene43479:CDS 50.0%
GATGTAATGCAGGGCTCCAT+AGG - chr8.4:240524-240543 None:intergenic 50.0%
GGCTCTATACGAAGCTTTGC+AGG - chr8.4:241637-241656 None:intergenic 50.0%
GTCACACCAACCTTTCCTTG+AGG - chr8.4:242707-242726 None:intergenic 50.0%
GTCGAGGCTTACACACCAAA+TGG + chr8.4:240420-240439 MS.gene43479:CDS 50.0%
GTTGGAGATTGGGTGGAAGT+GGG - chr8.4:240198-240217 None:intergenic 50.0%
TGCACTCATGGCTCATGTCA+TGG - chr8.4:240786-240805 None:intergenic 50.0%
TGTTGGAGATTGGGTGGAAG+TGG - chr8.4:240199-240218 None:intergenic 50.0%
!! AAGGTTGGTGTGACTGGAAG+TGG + chr8.4:242713-242732 MS.gene43479:CDS 50.0%
!! AGGTTGGTGTGACTGGAAGT+GGG + chr8.4:242714-242733 MS.gene43479:CDS 50.0%
!! CAAGGAAAGGTTGGTGTGAC+TGG + chr8.4:242707-242726 MS.gene43479:CDS 50.0%
!! TCTATCTCATGGCGGACATC+AGG - chr8.4:239959-239978 None:intergenic 50.0%
ACCACATCCTCAGGGTCATC+GGG - chr8.4:241658-241677 None:intergenic 55.0%
ATGCAGGGCTCCATAGGACT+AGG - chr8.4:240518-240537 None:intergenic 55.0%
CACCACATCCTCAGGGTCAT+CGG - chr8.4:241659-241678 None:intergenic 55.0%
CATGGAAAGAGAGCTCTCCG+AGG + chr8.4:239715-239734 MS.gene43479:CDS 55.0%
GCAGCAGTTCCAGACAACCA+AGG + chr8.4:242004-242023 MS.gene43479:CDS 55.0%
GGTGTGACTGGAAGTGGGAA+AGG + chr8.4:242719-242738 MS.gene43479:CDS 55.0%
GTGGTATGATGCAACCGTCG+AGG + chr8.4:240404-240423 MS.gene43479:CDS 55.0%
TATAGAGCCCGATGACCCTG+AGG + chr8.4:241648-241667 MS.gene43479:CDS 55.0%
TCCTGTTGGCACCAGAGTTC+AGG + chr8.4:240359-240378 MS.gene43479:CDS 55.0%
!! GTGGGTGGGTGTTGGAGATT+GGG - chr8.4:240208-240227 None:intergenic 55.0%
!! TGTGGGTGGGTGTTGGAGAT+TGG - chr8.4:240209-240228 None:intergenic 55.0%
! GCCTGAACTCTGGTGCCAAC+AGG - chr8.4:240363-240382 None:intergenic 60.0%
!! CTCATGGCGGACATCAGGAC+AGG - chr8.4:239954-239973 None:intergenic 60.0%
!! GGTGGGTGTTGGAGATTGGG+TGG - chr8.4:240205-240224 None:intergenic 60.0%
GCCCGATGACCCTGAGGATG+TGG + chr8.4:241654-241673 MS.gene43479:CDS 65.0%
GGCGGACATCAGGACAGGAC+AGG - chr8.4:239949-239968 None:intergenic 65.0%
!! GCAAGGTGGTAGCGGGCGTG+AGG + chr8.4:239478-239497 MS.gene43479:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 239476 242811 239476 ID=MS.gene43479
chr8.4 mRNA 239476 242811 239476 ID=MS.gene43479.t1;Parent=MS.gene43479
chr8.4 exon 239476 239571 239476 ID=MS.gene43479.t1.exon1;Parent=MS.gene43479.t1
chr8.4 CDS 239476 239571 239476 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
chr8.4 exon 239668 239736 239668 ID=MS.gene43479.t1.exon2;Parent=MS.gene43479.t1
chr8.4 CDS 239668 239736 239668 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
chr8.4 exon 240144 240236 240144 ID=MS.gene43479.t1.exon3;Parent=MS.gene43479.t1
chr8.4 CDS 240144 240236 240144 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
chr8.4 exon 240324 240482 240324 ID=MS.gene43479.t1.exon4;Parent=MS.gene43479.t1
chr8.4 CDS 240324 240482 240324 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
chr8.4 exon 241490 241675 241490 ID=MS.gene43479.t1.exon5;Parent=MS.gene43479.t1
chr8.4 CDS 241490 241675 241490 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
chr8.4 exon 241912 242040 241912 ID=MS.gene43479.t1.exon6;Parent=MS.gene43479.t1
chr8.4 CDS 241912 242040 241912 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
chr8.4 exon 242650 242811 242650 ID=MS.gene43479.t1.exon7;Parent=MS.gene43479.t1
chr8.4 CDS 242650 242811 242650 ID=cds.MS.gene43479.t1;Parent=MS.gene43479.t1
Gene Sequence

>MS.gene43479

ATGCAAGGTGGTAGCGGGCGTGAGGACGAAGAAGAAGAATTAAATATAAGTGAACTCGCATCCAATCTTTCTACTTACAAACAACAACTTCATCAGGTTTGTTTGTTTGTTTGTTTTTTTAGGGTTAGGGTTAGGGTTAGGGTTTCTCTTTTTCTCTTTTTCTGATTAATTTTGATTCCCTTCTCTTTTCAGGTTAGAGAACTTCTGAATGATGAACCATCTAATTCTGAGTATGTCGACATGGAAAGAGAGCTCTCCGAGGTTTTTCTTTTCTTTTCTTTCATAACTTGCTATGCTATGCTCATTTCATTTGTTTTAGCTTCAAATTGAAATTGAAAAGGTGTGAGTGAGCTTAGATCCCATTCAATTAATAATAACAACCCATTATTAATTAACAAGAAAACAAAATCATAATCAAACAAAAAAAGCAAAATTCCCATCACTTCTATCTATCTTGCTTCTGTGCAGGTCCTGTCCTGTCCTGATGTCCGCCATGAGATAGATACATACAACACACAATTACAGACAGAGATTCTAAGTTACCAACAACATTATTTATCTATTTTGCTTTATAATATCTATTATCTCATTCCAACTCTCACTAATATTAATTATATTATTATTACTTTATTCCAATTCCAATGTCTCCTTAAATTCTACAACACCAGGTCATTGCTTTGACTGAAGAACTTCTTGCCACTGCTAAGCAAAATGAAATACCCACTTCCACCCAATCTCCAACACCCACCCACAATAAACTGGTATGTACGTTTTTGTTTATTCATTCATATTCATTCCTAATCTATTCATTACTCATCATCTTTATCTATGTTTTTCACTGTTTTTAGGACCTTCATACCCACTTTGACCATCAACACAAATTTCCTGTTGGCACCAGAGTTCAGGCTGTTTATAGTGATGATGGGGAGTGGTATGATGCAACCGTCGAGGCTTACACACCAAATGGTTATTATGTCAGCTATGACACTTGGGGTAATAAAGAAGAGGTATACTTTCGCTCGCAGCCACTTGATGCAATCCTAGTCCTATGGAGCCCTGCATTACATCTGCATACATTTTCCTCCAATTTATTTACCATTGCATTATCTATGTCGTACCTTTTTTTTTTCTTTCCATGTTAGAGAAACAGAGTTGGAGATTAAGTTCTAATGAAAGTTCTATGTGATTTTTGCTGGATCTATATTTAACAACGACAATATTAAGAAAGAAATCCACAATTGCATTTCCAACTTATTGCATCTGTATTGTTATGTTGTATCTCCGTTTTTTTCTTCAGCATTATAACTCCATGACATGAGCCATGAGTGCATGATATATTTGGTTTGCAAAAACACAAACTCATTATAAAATTTTCTGGATGTTGTGAATGAGTATCAAATCATGTAACAGGTTATATCAAGTAGTAAGCATCCCATCACGTTTATTGAAGCGTTTTCCAGTTTTTTGTTTAGTTGAGAAAAGAAATAGAGGAATCACTTTATCCTAGGGAGGCTTACTTCCAAGTTGAGAAATCTCATTCCTGTACTTTGTGTGCTACTATGCGTGTGTATTGAATTGTTTTGCAGCACCTTGTTGAAATGGAAAGAACAATAAAAAGTATTTTACTGGAGAAATTAATTTTTAACATTCCTCACTCAGCATCAACAGAATGTATCAATTATCATCTAAAACATTAATGTTTGCTATTGAAAGGGTTAAATTTGTTTGTAGTCCCTATAGAATTATGAATCTGTCTAGTTTCGTCCCTATATATATTTTTTGGACCCTTTTTAGTTTCCTACCTTTTATATAAACAAGTGTTCTGTACTCCTTAGAGAGGGACTAAAAGTACCTGTTTTTATAAAATGTAGGGGCTAAAAATTGTTTATAGGACTAAAATAAAAATGAATATTTTGTAGGGACTAAACATATTTAACCCTATAAAAAAAAAATACTCCAGTGGTTTAGTTACTGATTTTCAAGGTAAACTTCATATGTTGCTTGCTTATTACAGGTAGATCCCGCTAACATAAGGCCAATTCAAGAAGGAACTGTTGATCCTTTGGTTGAAGCTGAGCGAGTTGCTGAAGCTACAAAGCAGGCTATCAAACGGAAAATTGCGCAAGCGGCATCTGTTGATTTTCAGTCACGGAGTCTACCTGCAAAGCTTCGTATAGAGCCCGATGACCCTGAGGATGTGGTGAGTTGTGTGCTGAAATGTTCACTTGATAATAAAAATCTTATTAAATAACTGCTGTCTTAATAAATATTTTCCTTGGTTTGATCTCTTGTTCTATTGTTTAATTTTAGTGAAGTGTTTGTTTTTCTGCAAATGTATTTCTTGGTAGTGGCTTCTGAGTTTATTATGTTTAATCCAAATTTATCTGCCAGTTTTCTAGGATTCCCTAAATGACATGTTTGCTAACTTGATTACAGAAAGTTACCAAACGTAAGAAAATACATGCCTTTAAGTCTAAGATGCGGATGGAACAACTTGAAGTCACCCAAAATAAGCGGCAAAATGCTTGGCAGCAGTTCCAGACAACCAAGGGAAAAGCAAAAAAGGTACCTTTCTCAATTTCTCTACTATTTAGTCACTCATTTCTTTTAGTGTTGAGAAGTTTCAGAGCTTGTAGTTTCTATGCAAGAATGTAGGAATTTGTTTTGTATGTCTTAAAGTGACTAATGGGAATGTACTATCACCTCCATTTTTGACCATCCTAACATACGAGTCAATGTCAGCTTATATATTTAGTGAAGAATTTTATGTTTTGTTCTACTCTCTTGAGTTGAATTTGTTCTAGCCTGAATTTACCACAGGAGAGAAGTATCATTGCAGTCATTAAGTTGGAAGTCAGATGAATTATAATATATATTATAATCAAATGATTTATTCTGATTAAAATTCCATGTGAAAATATTTTTTAGCTAAATTTCAAAAATCTATTTACCCCTTGAATCGGTTGTAAGTTGGATAGGAATGAGGAGGAAAAGTAATACCATCAACCCTGGAAAAGTTGTTGAATTTATTGAAAAAAGAATATAAGGCCCGATCTCTAGAATTTTCACAATCCACTGATATTTGTGTTTTATATAAATTTAGTTTGTTATGTTTACGTTGTTTTCATATAATTGTTCTCTTTTGTGATGTATTCACTGTGGTTTTGCTTCAAGATTGGTTTCTTCTCGGGAAGGAAGCGTGAGAGTATTTTTAAATCTCCTGATGATCCTCAAGGAAAGGTTGGTGTGACTGGAAGTGGGAAAGGTTTGACAGAGTTCCAGAGAAGAGAAAAGCATTTTCATCTGAAGGATGGTACAATCGAGAATGATGATTAG

Protein sequence

>MS.gene43479.t1

MQGGSGREDEEEELNISELASNLSTYKQQLHQVRELLNDEPSNSEYVDMERELSEVIALTEELLATAKQNEIPTSTQSPTPTHNKLDLHTHFDHQHKFPVGTRVQAVYSDDGEWYDATVEAYTPNGYYVSYDTWGNKEEVDPANIRPIQEGTVDPLVEAERVAEATKQAIKRKIAQAASVDFQSRSLPAKLRIEPDDPEDVKVTKRKKIHAFKSKMRMEQLEVTQNKRQNAWQQFQTTKGKAKKIGFFSGRKRESIFKSPDDPQGKVGVTGSGKGLTEFQRREKHFHLKDGTIENDD