AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43552

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43552.t1 MTR_5g015720 96.163 417 16 0 1 417 1 417 0.0 798
MS.gene43552.t1 MTR_1g056640 28.176 433 266 12 23 414 62 490 1.23e-40 151
MS.gene43552.t1 MTR_1g116870 60.215 93 35 2 323 415 270 360 6.70e-31 122
MS.gene43552.t1 MTR_3g082080 60.215 93 35 2 324 416 152 242 1.41e-30 118
MS.gene43552.t1 MTR_5g085580 54.651 86 38 1 329 414 382 466 1.27e-26 111
MS.gene43552.t1 MTR_1g084820 29.167 456 222 20 37 408 58 496 6.49e-25 107
MS.gene43552.t1 MTR_5g097420 54.545 88 39 1 327 414 28 114 1.69e-24 97.8
MS.gene43552.t1 MTR_1g084820 29.386 456 221 21 37 408 58 496 7.64e-24 104
MS.gene43552.t1 MTR_8g085390 52.326 86 39 2 329 414 30 113 1.43e-23 97.4
MS.gene43552.t1 MTR_7g111600 53.488 86 38 2 329 414 160 243 4.03e-23 98.2
MS.gene43552.t1 MTR_8g085720 49.425 87 43 1 328 414 363 448 7.96e-23 100
MS.gene43552.t1 MTR_7g111600 53.488 86 38 2 329 414 160 243 1.18e-22 97.8
MS.gene43552.t1 MTR_7g111600 37.766 188 92 6 246 414 62 243 2.07e-22 97.4
MS.gene43552.t1 MTR_4g133735 50.000 86 42 1 329 414 28 112 2.63e-22 91.7
MS.gene43552.t1 MTR_3g048280 52.500 80 36 2 329 408 112 189 5.93e-22 93.6
MS.gene43552.t1 MTR_8g089400 51.163 86 41 1 329 414 37 121 6.01e-22 92.0
MS.gene43552.t1 MTR_8g089400 51.163 86 41 1 329 414 57 141 1.86e-21 91.3
MS.gene43552.t1 MTR_8g012400 45.455 88 47 1 327 414 372 458 2.70e-21 95.9
MS.gene43552.t1 MTR_8g012400 45.455 88 47 1 327 414 292 378 3.27e-21 95.1
MS.gene43552.t1 MTR_5g091650 50.000 86 41 2 329 414 108 191 6.29e-21 90.5
MS.gene43552.t1 MTR_8g085400 50.000 88 39 3 322 409 26 108 1.28e-20 87.0
MS.gene43552.t1 MTR_5g091650 50.000 86 41 2 329 414 108 191 1.47e-20 91.3
MS.gene43552.t1 MTR_4g132280 48.837 86 43 1 329 414 382 466 2.31e-20 93.6
MS.gene43552.t1 MTR_4g133730 45.455 88 43 2 329 414 28 112 5.13e-20 85.5
MS.gene43552.t1 MTR_8g085440 46.591 88 45 2 327 414 30 115 5.61e-20 85.5
MS.gene43552.t1 MTR_4g088155 23.394 436 255 16 28 414 52 457 1.40e-19 90.9
MS.gene43552.t1 MTR_7g053500 46.591 88 46 1 327 414 295 381 2.34e-19 89.7
MS.gene43552.t1 MTR_7g053500 46.591 88 46 1 327 414 372 458 2.74e-19 90.1
MS.gene43552.t1 MTR_4g079530 45.652 92 49 1 323 414 31 121 6.03e-19 82.8
MS.gene43552.t1 MTR_3g083580 45.455 88 47 1 327 414 360 446 8.72e-19 88.6
MS.gene43552.t1 MTR_4g079530 46.067 89 47 1 326 414 34 121 1.54e-18 83.2
MS.gene43552.t1 MTR_2g049670 51.685 89 40 3 329 417 158 243 1.63e-18 86.3
MS.gene43552.t1 MTR_8g102340 42.222 90 51 1 326 415 331 419 4.95e-18 86.3
MS.gene43552.t1 MTR_4g109490 49.462 93 40 4 324 414 19 106 4.99e-18 82.8
MS.gene43552.t1 MTR_1g031900 44.944 89 48 1 326 414 358 445 9.25e-18 85.5
MS.gene43552.t1 MTR_4g069940 40.000 110 62 2 308 414 351 459 1.18e-17 85.1
MS.gene43552.t1 MTR_4g069940 30.380 158 105 2 25 177 55 212 6.84e-11 64.3
MS.gene43552.t1 MTR_1g031900 45.455 88 47 1 327 414 359 445 1.21e-17 85.1
MS.gene43552.t1 MTR_6g088885 43.182 88 48 2 329 415 369 455 1.62e-17 84.7
MS.gene43552.t1 MTR_8g085420 45.783 83 43 2 327 409 30 110 1.88e-17 79.0
MS.gene43552.t1 MTR_4g083500 44.318 88 47 2 327 414 400 485 5.74e-17 83.2
MS.gene43552.t1 MTR_8g088120 51.948 77 35 2 341 417 60 134 1.90e-16 78.6
MS.gene43552.t1 MTR_5g078200 42.045 88 50 1 327 414 325 411 2.08e-16 81.6
MS.gene43552.t1 MTR_5g078200 42.045 88 50 1 327 414 358 444 2.21e-16 81.6
MS.gene43552.t1 MTR_5g044530 50.685 73 34 2 328 400 21 91 4.55e-16 78.2
MS.gene43552.t1 MTR_4g083070 47.945 73 36 2 328 400 20 90 2.61e-15 74.3
MS.gene43552.t1 MTR_3g065460 42.529 87 47 2 329 414 361 445 1.13e-14 76.3
MS.gene43552.t1 MTR_1g022160 44.186 86 46 2 329 414 391 474 2.03e-14 75.1
MS.gene43552.t1 MTR_3g116510 41.860 86 48 2 329 414 237 320 2.70e-14 73.9
MS.gene43552.t1 MTR_3g116510 41.860 86 48 2 329 414 371 454 2.85e-14 74.7
MS.gene43552.t1 MTR_1g022160 43.023 86 47 2 329 414 391 474 3.87e-14 74.3
MS.gene43552.t1 MTR_2g072500 39.080 87 50 1 326 412 5 88 8.74e-13 64.3
MS.gene43552.t1 MTR_1g022160 44.304 79 42 2 329 407 391 467 1.60e-12 69.3
MS.gene43552.t1 MTR_7g026340 35.000 100 63 2 314 413 350 447 3.30e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43552.t1 AT5G67460 42.657 429 176 16 6 417 5 380 5.08e-85 265
MS.gene43552.t1 AT5G67460 42.080 423 175 16 6 411 5 374 1.41e-79 250
MS.gene43552.t1 AT5G55180 28.199 422 257 12 23 414 51 456 8.32e-39 145
MS.gene43552.t1 AT4G26830 27.423 423 256 11 23 414 49 451 1.58e-37 142
MS.gene43552.t1 AT4G26830 27.423 423 256 11 23 414 53 455 2.17e-37 141
MS.gene43552.t1 AT5G55180 28.365 416 252 12 23 408 51 450 6.36e-37 140
MS.gene43552.t1 AT5G56590 26.794 418 270 12 22 415 49 454 1.20e-32 129
MS.gene43552.t1 AT1G79480 58.065 93 37 2 322 414 303 393 1.06e-30 122
MS.gene43552.t1 AT1G79480 58.065 93 37 2 322 414 304 394 1.06e-30 122
MS.gene43552.t1 AT2G05790 58.140 86 35 1 329 414 384 468 2.93e-27 113
MS.gene43552.t1 AT2G30933 57.143 91 37 2 324 414 77 165 3.66e-26 105
MS.gene43552.t1 AT2G30933 58.824 85 33 2 324 408 77 159 7.33e-25 100
MS.gene43552.t1 AT2G30933 58.824 85 33 2 324 408 77 159 7.33e-25 100
MS.gene43552.t1 AT5G35740 48.837 86 44 0 329 414 30 115 1.93e-24 97.8
MS.gene43552.t1 AT3G07320 24.299 428 265 15 24 414 50 455 3.25e-24 104
MS.gene43552.t1 AT1G66855 44.444 90 49 1 325 414 22 110 1.28e-22 92.4
MS.gene43552.t1 AT2G04910 47.674 86 44 1 329 414 15 99 6.16e-22 90.5
MS.gene43552.t1 AT4G05430 46.512 86 45 1 329 414 23 107 3.06e-21 89.7
MS.gene43552.t1 AT2G04910 48.750 80 40 1 329 408 15 93 3.19e-21 89.0
MS.gene43552.t1 AT1G11820 48.864 88 44 1 327 414 379 465 3.66e-21 95.9
MS.gene43552.t1 AT4G05430 46.512 86 45 1 329 414 48 132 4.18e-21 90.1
MS.gene43552.t1 AT4G13600 49.425 87 42 2 328 414 60 144 6.07e-21 91.3
MS.gene43552.t1 AT1G29380 54.023 87 36 3 329 414 148 231 7.02e-21 92.0
MS.gene43552.t1 AT1G29380 54.023 87 36 3 329 414 148 231 8.16e-21 92.8
MS.gene43552.t1 AT1G66870 43.182 88 49 1 327 414 24 110 9.09e-21 87.4
MS.gene43552.t1 AT1G66870 43.182 88 49 1 327 414 51 137 1.69e-20 87.4
MS.gene43552.t1 AT1G11820 50.000 84 41 1 327 410 379 461 2.44e-20 93.2
MS.gene43552.t1 AT1G18650 51.163 86 40 2 329 414 21 104 3.33e-20 88.2
MS.gene43552.t1 AT5G61130 48.837 86 42 2 329 414 21 104 3.43e-20 88.6
MS.gene43552.t1 AT3G13560 46.667 90 47 1 328 417 361 449 4.11e-20 92.8
MS.gene43552.t1 AT3G13560 46.667 90 47 1 328 417 361 449 4.11e-20 92.8
MS.gene43552.t1 AT3G13560 46.667 90 47 1 328 417 361 449 4.11e-20 92.8
MS.gene43552.t1 AT3G13560 46.667 90 47 1 328 417 361 449 4.11e-20 92.8
MS.gene43552.t1 AT3G13560 46.667 90 47 1 328 417 361 449 4.11e-20 92.8
MS.gene43552.t1 AT4G05430 48.750 80 40 1 329 408 48 126 4.48e-20 85.9
MS.gene43552.t1 AT1G18650 51.163 86 40 2 329 414 21 104 4.74e-20 86.3
MS.gene43552.t1 AT1G29380 55.556 81 32 3 329 408 148 225 7.02e-20 88.2
MS.gene43552.t1 AT1G29380 55.556 81 32 3 329 408 148 225 7.02e-20 88.2
MS.gene43552.t1 AT2G01630 45.455 88 47 1 327 414 358 444 1.41e-19 91.3
MS.gene43552.t1 AT1G09460 51.163 86 38 3 329 414 137 218 3.50e-19 88.6
MS.gene43552.t1 AT2G01630 45.783 83 44 1 327 409 358 439 6.38e-19 89.0
MS.gene43552.t1 AT5G08000 48.889 90 44 2 325 414 36 123 2.00e-18 84.0
MS.gene43552.t1 AT5G08000 50.000 86 41 2 329 414 21 104 4.03e-18 82.4
MS.gene43552.t1 AT4G29360 35.433 127 81 1 291 417 353 478 5.75e-18 86.3
MS.gene43552.t1 AT4G29360 31.544 149 94 3 24 166 52 198 1.03e-11 67.0
MS.gene43552.t1 AT4G29360 35.433 127 81 1 291 417 363 488 6.16e-18 86.3
MS.gene43552.t1 AT4G29360 31.544 149 94 3 24 166 62 208 1.10e-11 67.0
MS.gene43552.t1 AT5G63225 46.429 84 43 2 329 412 25 106 9.21e-18 79.0
MS.gene43552.t1 AT5G63230 42.045 88 49 2 327 414 24 109 1.70e-17 78.2
MS.gene43552.t1 AT1G66852 39.773 88 51 2 327 414 24 109 2.43e-17 77.8
MS.gene43552.t1 AT3G55430 39.535 86 50 2 329 414 366 449 4.71e-17 83.2
MS.gene43552.t1 AT5G63250 41.284 109 54 4 309 415 29 129 2.71e-16 75.5
MS.gene43552.t1 AT2G43670 41.667 84 46 2 329 412 35 115 3.22e-16 75.1
MS.gene43552.t1 AT4G16165 35.227 88 56 1 327 414 24 110 5.24e-16 73.9
MS.gene43552.t1 AT5G53610 45.349 86 44 2 329 414 28 110 6.92e-16 73.6
MS.gene43552.t1 AT4G09090 40.230 87 50 2 329 415 31 115 8.67e-16 73.6
MS.gene43552.t1 AT2G29735 41.111 90 49 3 327 414 29 116 1.02e-15 73.6
MS.gene43552.t1 AT4G09462 38.636 88 52 2 327 414 27 112 1.61e-15 72.8
MS.gene43552.t1 AT4G09465 38.636 88 52 2 327 414 29 114 1.66e-15 72.8
MS.gene43552.t1 AT4G09467 38.636 88 52 2 327 414 29 114 1.90e-15 72.8
MS.gene43552.t1 AT4G09464 38.636 88 52 2 327 414 29 114 1.90e-15 72.8
MS.gene43552.t1 AT4G34480 43.023 86 47 2 329 414 253 336 2.82e-15 77.4
MS.gene43552.t1 AT4G34480 43.023 86 47 2 329 414 364 447 2.96e-15 77.8
MS.gene43552.t1 AT4G09466 37.500 88 53 2 327 414 29 114 5.99e-15 71.2
MS.gene43552.t1 AT1G66250 39.326 89 53 1 326 414 365 452 1.24e-14 75.9
MS.gene43552.t1 AT2G16230 37.864 103 61 3 313 414 349 449 2.02e-14 75.5
MS.gene43552.t1 AT3G23770 39.080 87 52 1 328 414 386 471 2.07e-14 75.1
MS.gene43552.t1 AT5G53600 36.559 93 56 2 322 414 22 111 3.04e-14 69.3
MS.gene43552.t1 AT1G69295 48.611 72 34 3 329 400 21 89 4.28e-14 71.6
MS.gene43552.t1 AT3G58100 39.773 88 52 1 327 414 39 125 5.15e-14 70.5
MS.gene43552.t1 AT1G69295 48.611 72 34 3 329 400 17 85 5.70e-14 71.2
MS.gene43552.t1 AT1G26450 53.226 62 27 2 341 402 32 91 5.81e-14 70.9
MS.gene43552.t1 AT3G58100 39.773 88 52 1 327 414 115 201 9.86e-14 71.2
MS.gene43552.t1 AT2G43660 36.957 92 57 1 323 414 29 119 2.12e-13 67.0
MS.gene43552.t1 AT5G63240 32.039 103 67 2 315 415 26 127 2.12e-13 67.4
MS.gene43552.t1 AT2G43660 36.957 92 57 1 323 414 28 118 2.17e-13 67.0
MS.gene43552.t1 AT5G24318 37.736 106 59 4 313 414 214 316 6.15e-13 69.7
MS.gene43552.t1 AT5G24318 37.736 106 59 4 313 414 353 455 6.36e-13 70.5
MS.gene43552.t1 AT1G13830 42.105 76 42 2 325 400 31 104 1.35e-12 67.0
MS.gene43552.t1 AT1G13830 43.836 73 39 2 328 400 20 90 2.17e-12 66.2
MS.gene43552.t1 AT1G78520 32.000 100 65 1 315 414 18 114 3.54e-12 63.5
MS.gene43552.t1 AT5G24318 38.835 103 56 4 313 411 353 452 6.88e-12 67.4
MS.gene43552.t1 AT3G28250 40.000 80 45 2 329 408 3 79 1.15e-11 62.0
MS.gene43552.t1 AT2G03505 38.750 80 47 2 329 408 21 98 2.05e-11 62.8
MS.gene43552.t1 AT2G03505 38.750 80 47 2 329 408 21 98 2.05e-11 62.8
MS.gene43552.t1 AT2G03505 38.750 80 47 2 329 408 21 98 2.05e-11 62.8
MS.gene43552.t1 AT2G03505 38.750 80 47 2 329 408 21 98 2.33e-11 61.2
MS.gene43552.t1 AT2G42930 37.113 97 58 3 312 408 23 116 4.02e-11 60.8

Find 79 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGTTTGCATTGCAGTTTC+TGG 0.188078 5.3:+8614622 MS.gene43552:intron
TTGTTCTTGAGGGGCAAAAT+AGG 0.257528 5.3:-8615406 None:intergenic
CTTATGCATTCAATAGTTAC+TGG 0.260747 5.3:+8615712 MS.gene43552:CDS
CCTTGCAATGCAATAGAAAA+TGG 0.301168 5.3:+8615495 MS.gene43552:CDS
TTTAAATTTGGTACATTGTA+AGG 0.323811 5.3:-8615330 None:intergenic
AAGAAGACAGAACAACATTA+AGG 0.332742 5.3:-8614885 None:intergenic
CATCTTCTCTTTCTTTGCTC+TGG 0.345679 5.3:+8614432 MS.gene43552:CDS
CCTGCTAACTCTCCTCAAAA+TGG 0.348344 5.3:+8615459 MS.gene43552:CDS
CACTTGTTTCACATAGGTTT+AGG 0.352249 5.3:-8614663 None:intergenic
ATGGTGATGGTTGTTCTTGA+GGG 0.357121 5.3:-8615416 None:intergenic
CAATGTTTATGATGTTGTTA+TGG 0.359003 5.3:-8615173 None:intergenic
GTTTGAAGAAACTCAATCAT+AGG 0.360523 5.3:-8615036 None:intergenic
GCATGAGCAACCAATGTATC+AGG 0.366680 5.3:-8615687 None:intergenic
TAAGGTTGTCTTGGTCTTGT+TGG 0.366840 5.3:-8614867 None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG 0.371125 5.3:+8614398 None:intergenic
GGTTTGAAGTAGCTGAGTTA+AGG 0.381419 5.3:-8614993 None:intergenic
GTTGAGCAATTTCTGGAATC+GGG 0.381769 5.3:-8615269 None:intergenic
TGAAACGTTGCAAGAAGCTT+TGG 0.385008 5.3:+8615596 MS.gene43552:CDS
TGTTCTTGAGGGGCAAAATA+GGG 0.397503 5.3:-8615405 None:intergenic
GGTTGAGCAATTTCTGGAAT+CGG 0.405946 5.3:-8615270 None:intergenic
GTTTGAAGTAGCTGAGTTAA+GGG 0.410988 5.3:-8614992 None:intergenic
TGGCAAAAGCATAAGAGAAT+TGG 0.415770 5.3:+8615732 MS.gene43552:CDS
GCTTCTTGCAACGTTTCATC+AGG 0.416313 5.3:-8615591 None:intergenic
TTCTGAACAGGTGCAATTTG+TGG 0.425677 5.3:-8615540 None:intergenic
TTGCACCTGTTCAGAAACTT+TGG 0.430193 5.3:+8615547 MS.gene43552:CDS
GCAAAATAGGGAGAAGAAGT+TGG 0.438544 5.3:-8615393 None:intergenic
GTTCTAAACCCCATCGTTTG+AGG 0.447502 5.3:-8614927 None:intergenic
GATGAAACTATTTGAGCTAA+AGG 0.452012 5.3:-8615363 None:intergenic
TATCAGCTGAAACTTGGCTT+AGG 0.457511 5.3:+8614776 MS.gene43552:CDS
TTGCAGGTTTCCTTCACTGT+AGG 0.460667 5.3:+8616123 MS.gene43552:intron
TTTGGTACATTGTAAGGAGC+TGG 0.460933 5.3:-8615324 None:intergenic
TTCTAAACCCCATCGTTTGA+GGG 0.462743 5.3:-8614926 None:intergenic
ATTTGAGCTAAAGGTGGTAA+AGG 0.464246 5.3:-8615354 None:intergenic
TTGGATTATGCTTGTGGTGA+AGG 0.471347 5.3:+8615615 MS.gene43552:CDS
AATGGTGATGGTTGTTCTTG+AGG 0.476899 5.3:-8615417 None:intergenic
ATTGGTGGAACTTGTGACTT+TGG 0.478733 5.3:+8615750 MS.gene43552:CDS
ACACACCAAAGTTTCTGAAC+AGG 0.478993 5.3:-8615552 None:intergenic
TTGTTACAATCCTGATACAT+TGG 0.480675 5.3:+8615677 MS.gene43552:CDS
GTGGAACTTGTGACTTTGGT+GGG 0.482171 5.3:+8615754 MS.gene43552:CDS
TGTTGCTAGAGACCTTGTTA+AGG 0.501164 5.3:-8614750 None:intergenic
ACATTGTAAGGAGCTGGATA+AGG 0.502108 5.3:-8615318 None:intergenic
GCAATTTCTGGAATCGGGGT+TGG 0.502513 5.3:-8615264 None:intergenic
GAACAACATTAAGGTTGTCT+TGG 0.506879 5.3:-8614876 None:intergenic
CAAAAGCATAAGAGAATTGG+TGG 0.510123 5.3:+8615735 MS.gene43552:CDS
AAATGTGAAAACATTGAACT+TGG 0.513995 5.3:-8615084 None:intergenic
CTATCATTCCCTCAAACGAT+GGG 0.516242 5.3:+8614918 MS.gene43552:CDS
TCTATCATTCCCTCAAACGA+TGG 0.523791 5.3:+8614917 MS.gene43552:CDS
TAGGGAGAAGAAGTTGGTGG+TGG 0.524934 5.3:-8615387 None:intergenic
TTGTTCAAAGAAACAGCTAA+AGG 0.528055 5.3:-8614721 None:intergenic
GATACAAACTGTGGTGGTAA+TGG 0.537334 5.3:-8615435 None:intergenic
AACCAGCAGGGAAATCCAAA+GGG 0.537740 5.3:-8615293 None:intergenic
GAACCAGCAGGGAAATCCAA+AGG 0.539386 5.3:-8615294 None:intergenic
GCTGGATAAGGAGAACCAGC+AGG 0.554077 5.3:-8615306 None:intergenic
TGTCTTCACTTGTTTCACAT+AGG 0.568580 5.3:-8614669 None:intergenic
ACAGTCCAAATCAAAAGCAA+TGG 0.570988 5.3:-8614965 None:intergenic
GTCTTGTTGGCAGAAAGTTG+AGG 0.574769 5.3:-8614854 None:intergenic
GATTATGCTTGTGGTGAAGG+TGG 0.582581 5.3:+8615618 MS.gene43552:CDS
GGTGGAACTTGTGACTTTGG+TGG 0.584114 5.3:+8615753 MS.gene43552:CDS
AACGTTTCATCAGGAACACT+AGG 0.602336 5.3:-8615582 None:intergenic
GAAGCTTTGGATTATGCTTG+TGG 0.604890 5.3:+8615609 MS.gene43552:CDS
GGATTGTAACAATTCCCTTG+TGG 0.608596 5.3:-8615666 None:intergenic
TGAGAATAAACCTACAGTGA+AGG 0.617438 5.3:-8616133 None:intergenic
TTGAGCAATTTCTGGAATCG+GGG 0.619661 5.3:-8615268 None:intergenic
TTAGAAATTACTACTCCACA+AGG 0.626530 5.3:+8615651 MS.gene43552:CDS
AAAAGAAAATGAAAATGGTG+TGG 0.631353 5.3:+8614403 None:intergenic
AACTGTGGTGGTAATGGTGA+TGG 0.632946 5.3:-8615429 None:intergenic
GAAACTATTTGAGCTAAAGG+TGG 0.639226 5.3:-8615360 None:intergenic
TGGTGATGGTTGTTCTTGAG+GGG 0.643729 5.3:-8615415 None:intergenic
AAATAGGGAGAAGAAGTTGG+TGG 0.647085 5.3:-8615390 None:intergenic
GCAATTTGTGGATAATGTGA+AGG 0.661579 5.3:-8615528 None:intergenic
TGTGGATAATGTGAAGGTGA+AGG 0.662098 5.3:-8615522 None:intergenic
GAACAATGAAGACCTTAACA+AGG 0.665682 5.3:+8614738 MS.gene43552:CDS
CTGGATAAGGAGAACCAGCA+GGG 0.668605 5.3:-8615305 None:intergenic
GCAGGAGATACAAACTGTGG+TGG 0.675527 5.3:-8615441 None:intergenic
TATCATTCCCTCAAACGATG+GGG 0.690178 5.3:+8614919 MS.gene43552:CDS
TAGAAATTACTACTCCACAA+GGG 0.723314 5.3:+8615652 MS.gene43552:CDS
ACCAGCAGGGAAATCCAAAG+GGG 0.725497 5.3:-8615292 None:intergenic
CCAGCAGGGAAATCCAAAGG+GGG 0.739134 5.3:-8615291 None:intergenic
TTAGCAGGAGATACAAACTG+TGG 0.759496 5.3:-8615444 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAGTGCTTATTAATTT+AGG + chr5.3:8615803-8615822 MS.gene43552:intron 20.0%
!! TAAATTAATAAGCACTTACT+TGG - chr5.3:8615804-8615823 None:intergenic 20.0%
!! TGTTAATTAATAATGAGTGA+TGG + chr5.3:8615854-8615873 MS.gene43552:intron 20.0%
!! TTACATTAATAATGGTGAAT+TGG + chr5.3:8616089-8616108 MS.gene43552:intron 20.0%
!! TTTAAATTTGGTACATTGTA+AGG - chr5.3:8615333-8615352 None:intergenic 20.0%
!!! AATTAATTAGCTGTTTTAGA+TGG - chr5.3:8616038-8616057 None:intergenic 20.0%
!!! GTTTTAACATTTTCAAATCA+TGG - chr5.3:8615017-8615036 None:intergenic 20.0%
!!! TTTTCACATTTTTCTGATAA+AGG + chr5.3:8615096-8615115 MS.gene43552:CDS 20.0%
! AAATGTGAAAACATTGAACT+TGG - chr5.3:8615087-8615106 None:intergenic 25.0%
! ATAAAGTATGTAACTACCAA+AGG + chr5.3:8615985-8616004 MS.gene43552:intron 25.0%
! CAATGTTTATGATGTTGTTA+TGG - chr5.3:8615176-8615195 None:intergenic 25.0%
! TATAGTTATTGTACATAGCA+GGG - chr5.3:8615968-8615987 None:intergenic 25.0%
! TTATAGTTATTGTACATAGC+AGG - chr5.3:8615969-8615988 None:intergenic 25.0%
!! ATTTTTGATGAAGGGTAATA+TGG - chr5.3:8614814-8614833 None:intergenic 25.0%
!! TAGTGATTTTTCTCTCTTTT+GGG - chr5.3:8615209-8615228 None:intergenic 25.0%
!! TTTTTGATGAAGGGTAATAT+GGG - chr5.3:8614813-8614832 None:intergenic 25.0%
!!! CTTTTTCATGGATTCTAAAT+GGG - chr5.3:8615139-8615158 None:intergenic 25.0%
AAGAAGACAGAACAACATTA+AGG - chr5.3:8614888-8614907 None:intergenic 30.0%
CTTATGCATTCAATAGTTAC+TGG + chr5.3:8615712-8615731 MS.gene43552:CDS 30.0%
GATGAAACTATTTGAGCTAA+AGG - chr5.3:8615366-8615385 None:intergenic 30.0%
GTTTGAAGAAACTCAATCAT+AGG - chr5.3:8615039-8615058 None:intergenic 30.0%
TAGAAATTACTACTCCACAA+GGG + chr5.3:8615652-8615671 MS.gene43552:CDS 30.0%
TCAAAAATCACCAACATTGT+TGG + chr5.3:8614826-8614845 MS.gene43552:CDS 30.0%
TTAGAAATTACTACTCCACA+AGG + chr5.3:8615651-8615670 MS.gene43552:CDS 30.0%
TTAGAATCCATGAAAAAGCT+TGG + chr5.3:8615141-8615160 MS.gene43552:CDS 30.0%
TTGTTACAATCCTGATACAT+TGG + chr5.3:8615677-8615696 MS.gene43552:CDS 30.0%
TTGTTCAAAGAAACAGCTAA+AGG - chr5.3:8614724-8614743 None:intergenic 30.0%
! ACATTTTATCAGCTGAAACT+TGG + chr5.3:8614770-8614789 MS.gene43552:CDS 30.0%
! AGTTAGTTACACAGTTTGTT+AGG - chr5.3:8616011-8616030 None:intergenic 30.0%
! GTAGTGATTTTTCTCTCTTT+TGG - chr5.3:8615210-8615229 None:intergenic 30.0%
!! AGTTTCCATTGCTTTTGATT+TGG + chr5.3:8614960-8614979 MS.gene43552:CDS 30.0%
!! ATTGGTTATTGTTTTGTTGC+AGG + chr5.3:8616107-8616126 MS.gene43552:intron 30.0%
!!! ATGTTGGTGATTTTTGATGA+AGG - chr5.3:8614823-8614842 None:intergenic 30.0%
!!! GAAAAATCCAAGCTTTTTCA+TGG - chr5.3:8615151-8615170 None:intergenic 30.0%
!!! GCTTTTTCATGGATTCTAAA+TGG - chr5.3:8615140-8615159 None:intergenic 30.0%
!!! GGTGGTAAAGGTTTTAAATT+TGG - chr5.3:8615345-8615364 None:intergenic 30.0%
!!! TGTTGGTGATTTTTGATGAA+GGG - chr5.3:8614822-8614841 None:intergenic 30.0%
!!! TTTTCTCTCTTTTGGGATAA+TGG - chr5.3:8615202-8615221 None:intergenic 30.0%
ACAGTCCAAATCAAAAGCAA+TGG - chr5.3:8614968-8614987 None:intergenic 35.0%
ATTTGAGCTAAAGGTGGTAA+AGG - chr5.3:8615357-8615376 None:intergenic 35.0%
CACTTGTTTCACATAGGTTT+AGG - chr5.3:8614666-8614685 None:intergenic 35.0%
CCTTGCAATGCAATAGAAAA+TGG + chr5.3:8615495-8615514 MS.gene43552:CDS 35.0%
GAAACTATTTGAGCTAAAGG+TGG - chr5.3:8615363-8615382 None:intergenic 35.0%
GAACAACATTAAGGTTGTCT+TGG - chr5.3:8614879-8614898 None:intergenic 35.0%
GAACAATGAAGACCTTAACA+AGG + chr5.3:8614738-8614757 MS.gene43552:CDS 35.0%
GCAATTTGTGGATAATGTGA+AGG - chr5.3:8615531-8615550 None:intergenic 35.0%
TGAGAATAAACCTACAGTGA+AGG - chr5.3:8616136-8616155 None:intergenic 35.0%
TGTCTTCACTTGTTTCACAT+AGG - chr5.3:8614672-8614691 None:intergenic 35.0%
! CAAAAGCATAAGAGAATTGG+TGG + chr5.3:8615735-8615754 MS.gene43552:CDS 35.0%
! CCATTTTCTATTGCATTGCA+AGG - chr5.3:8615498-8615517 None:intergenic 35.0%
! GTTTGAAGTAGCTGAGTTAA+GGG - chr5.3:8614995-8615014 None:intergenic 35.0%
! TGAGGTTTTTCCAACAATGT+TGG - chr5.3:8614839-8614858 None:intergenic 35.0%
! TGGCAAAAGCATAAGAGAAT+TGG + chr5.3:8615732-8615751 MS.gene43552:CDS 35.0%
! TTTTCTATTGCATTGCAAGG+TGG - chr5.3:8615495-8615514 None:intergenic 35.0%
!!! GGTTGGTTTTATAGGAATTG+TGG - chr5.3:8615250-8615269 None:intergenic 35.0%
AAATAGGGAGAAGAAGTTGG+TGG - chr5.3:8615393-8615412 None:intergenic 40.0%
AACGTTTCATCAGGAACACT+AGG - chr5.3:8615585-8615604 None:intergenic 40.0%
ACACACCAAAGTTTCTGAAC+AGG - chr5.3:8615555-8615574 None:intergenic 40.0%
ACATTGTAAGGAGCTGGATA+AGG - chr5.3:8615321-8615340 None:intergenic 40.0%
AGTGATGGTTGTGTTGTGAA+TGG + chr5.3:8615869-8615888 MS.gene43552:intron 40.0%
ATTGGTGGAACTTGTGACTT+TGG + chr5.3:8615750-8615769 MS.gene43552:CDS 40.0%
CATCTTCTCTTTCTTTGCTC+TGG + chr5.3:8614432-8614451 MS.gene43552:CDS 40.0%
CTATCATTCCCTCAAACGAT+GGG + chr5.3:8614918-8614937 MS.gene43552:CDS 40.0%
GATACAAACTGTGGTGGTAA+TGG - chr5.3:8615438-8615457 None:intergenic 40.0%
GCAAAATAGGGAGAAGAAGT+TGG - chr5.3:8615396-8615415 None:intergenic 40.0%
GGATTGTAACAATTCCCTTG+TGG - chr5.3:8615669-8615688 None:intergenic 40.0%
TATCAGCTGAAACTTGGCTT+AGG + chr5.3:8614776-8614795 MS.gene43552:CDS 40.0%
TATCATTCCCTCAAACGATG+GGG + chr5.3:8614919-8614938 MS.gene43552:CDS 40.0%
TCTATCATTCCCTCAAACGA+TGG + chr5.3:8614917-8614936 MS.gene43552:CDS 40.0%
TGAAACGTTGCAAGAAGCTT+TGG + chr5.3:8615596-8615615 MS.gene43552:CDS 40.0%
TGTGGATAATGTGAAGGTGA+AGG - chr5.3:8615525-8615544 None:intergenic 40.0%
TGTTCTTGAGGGGCAAAATA+GGG - chr5.3:8615408-8615427 None:intergenic 40.0%
TGTTGCTAGAGACCTTGTTA+AGG - chr5.3:8614753-8614772 None:intergenic 40.0%
TTAGCAGGAGATACAAACTG+TGG - chr5.3:8615447-8615466 None:intergenic 40.0%
TTCTAAACCCCATCGTTTGA+GGG - chr5.3:8614929-8614948 None:intergenic 40.0%
TTCTGAACAGGTGCAATTTG+TGG - chr5.3:8615543-8615562 None:intergenic 40.0%
TTGCACCTGTTCAGAAACTT+TGG + chr5.3:8615547-8615566 MS.gene43552:CDS 40.0%
TTGGATTATGCTTGTGGTGA+AGG + chr5.3:8615615-8615634 MS.gene43552:CDS 40.0%
TTGTTCTTGAGGGGCAAAAT+AGG - chr5.3:8615409-8615428 None:intergenic 40.0%
TTTGGTACATTGTAAGGAGC+TGG - chr5.3:8615327-8615346 None:intergenic 40.0%
! GGACAAGTGAAACCATTTTG+AGG - chr5.3:8615474-8615493 None:intergenic 40.0%
! GGTTTGAAGTAGCTGAGTTA+AGG - chr5.3:8614996-8615015 None:intergenic 40.0%
! GTAGTTTGCATTGCAGTTTC+TGG + chr5.3:8614622-8614641 MS.gene43552:intron 40.0%
!! AATGGTGATGGTTGTTCTTG+AGG - chr5.3:8615420-8615439 None:intergenic 40.0%
!! ATGGTGATGGTTGTTCTTGA+GGG - chr5.3:8615419-8615438 None:intergenic 40.0%
!! GAAGCTTTGGATTATGCTTG+TGG + chr5.3:8615609-8615628 MS.gene43552:CDS 40.0%
!! GGTTGAGCAATTTCTGGAAT+CGG - chr5.3:8615273-8615292 None:intergenic 40.0%
!! GTTGAGCAATTTCTGGAATC+GGG - chr5.3:8615272-8615291 None:intergenic 40.0%
!! TAAGGTTGTCTTGGTCTTGT+TGG - chr5.3:8614870-8614889 None:intergenic 40.0%
!! TACACAGTTTGTTAGGCCTT+TGG - chr5.3:8616004-8616023 None:intergenic 40.0%
!! TTGAGCAATTTCTGGAATCG+GGG - chr5.3:8615271-8615290 None:intergenic 40.0%
AAAGGGGGTTGAGCAATTTC+TGG - chr5.3:8615279-8615298 None:intergenic 45.0%
AACCAGCAGGGAAATCCAAA+GGG - chr5.3:8615296-8615315 None:intergenic 45.0%
AGAAATTGCTCAACCCCCTT+TGG + chr5.3:8615278-8615297 MS.gene43552:CDS 45.0%
CCTGCTAACTCTCCTCAAAA+TGG + chr5.3:8615459-8615478 MS.gene43552:CDS 45.0%
GATTATGCTTGTGGTGAAGG+TGG + chr5.3:8615618-8615637 MS.gene43552:CDS 45.0%
GCATGAGCAACCAATGTATC+AGG - chr5.3:8615690-8615709 None:intergenic 45.0%
GCTTCTTGCAACGTTTCATC+AGG - chr5.3:8615594-8615613 None:intergenic 45.0%
GTGGAACTTGTGACTTTGGT+GGG + chr5.3:8615754-8615773 MS.gene43552:CDS 45.0%
TTGCAGGTTTCCTTCACTGT+AGG + chr5.3:8616123-8616142 MS.gene43552:intron 45.0%
! GTCTTGTTGGCAGAAAGTTG+AGG - chr5.3:8614857-8614876 None:intergenic 45.0%
! GTTCTAAACCCCATCGTTTG+AGG - chr5.3:8614930-8614949 None:intergenic 45.0%
!! AACTGTGGTGGTAATGGTGA+TGG - chr5.3:8615432-8615451 None:intergenic 45.0%
!! CCATTTTGAGGAGAGTTAGC+AGG - chr5.3:8615462-8615481 None:intergenic 45.0%
!! TGGTGATGGTTGTTCTTGAG+GGG - chr5.3:8615418-8615437 None:intergenic 45.0%
!!! GGAATCGGGGTTGGTTTTAT+AGG - chr5.3:8615258-8615277 None:intergenic 45.0%
!! TTATTTATTTACATTAATAA+TGG + chr5.3:8616081-8616100 MS.gene43552:intron 5.0%
ACCAGCAGGGAAATCCAAAG+GGG - chr5.3:8615295-8615314 None:intergenic 50.0%
CTGGATAAGGAGAACCAGCA+GGG - chr5.3:8615308-8615327 None:intergenic 50.0%
GAACCAGCAGGGAAATCCAA+AGG - chr5.3:8615297-8615316 None:intergenic 50.0%
GCAGGAGATACAAACTGTGG+TGG - chr5.3:8615444-8615463 None:intergenic 50.0%
GGTGGAACTTGTGACTTTGG+TGG + chr5.3:8615753-8615772 MS.gene43552:CDS 50.0%
!! GCAATTTCTGGAATCGGGGT+TGG - chr5.3:8615267-8615286 None:intergenic 50.0%
!! TAGGGAGAAGAAGTTGGTGG+TGG - chr5.3:8615390-8615409 None:intergenic 50.0%
CCAGCAGGGAAATCCAAAGG+GGG - chr5.3:8615294-8615313 None:intergenic 55.0%
GCTGGATAAGGAGAACCAGC+AGG - chr5.3:8615309-8615328 None:intergenic 55.0%
CCCCCTTTGGATTTCCCTGC+TGG + chr5.3:8615291-8615310 MS.gene43552:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 8614411 8616160 8614411 ID=MS.gene43552
chr5.3 mRNA 8614411 8616160 8614411 ID=MS.gene43552.t1;Parent=MS.gene43552
chr5.3 exon 8614411 8614453 8614411 ID=MS.gene43552.t1.exon1;Parent=MS.gene43552.t1
chr5.3 CDS 8614411 8614453 8614411 ID=cds.MS.gene43552.t1;Parent=MS.gene43552.t1
chr5.3 exon 8614626 8615804 8614626 ID=MS.gene43552.t1.exon2;Parent=MS.gene43552.t1
chr5.3 CDS 8614626 8615804 8614626 ID=cds.MS.gene43552.t1;Parent=MS.gene43552.t1
chr5.3 exon 8616129 8616160 8616129 ID=MS.gene43552.t1.exon3;Parent=MS.gene43552.t1
chr5.3 CDS 8616129 8616160 8616129 ID=cds.MS.gene43552.t1;Parent=MS.gene43552.t1
Gene Sequence

>MS.gene43552

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGGTAAGAAAAACAACTAAAATCTCAGTTTTGTAATTTCTCTTAAACTAAAATGTCTCAAAATATTCATCATCAATGTATCAATGCTTTAGTTTATTATTCTTCTTTTAAGTATTTTTTAACATTTTATTATTATTATTATTATCTAATGAAAGTTTTTGTTTTTTTGTTGTAGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAATGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCAGCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACCTCAACTTTCTGCCAACAAGACCAAGACAACCTTAATGTTGTTCTGTCTTCTTTGAAAAATGTCTATCATTCCCTCAAACGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCAAACCATGATTTGAAAATGTTAAAACCTATGATTGAGTTTCTTCAAACTGTTAACTCAACATTTTCTCTCATACCAAGTTCAATGTTTTCACATTTTTCTGATAAAGGTTTAAACTTTGTATCTTCCCATTTAGAATCCATGAAAAAGCTTGGATTTTTCAACCATAACAACATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCGATTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTTAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAACTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAATAGAAAATGGTTCACCTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGAAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTAGAAATTACTACTCCACAAGGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCATTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTGGGACTGCTATGTTGATTCATTCTGATCCAAGTAAGTGCTTATTAATTTAGGATTAATTACAATTTTTTTACTTATACTTTGTTAATTAATAATGAGTGATGGTTGTGTTGTGAATGGTATGAATTTGTGATAGAGAAAAAAGAACTTTTCATTGTTACTGAGAATGAAAAATATTGATACATATACTATACCCTGCTATGTACAATAACTATAAAGTATGTAACTACCAAAGGCCTAACAAACTGTGTAACTAACTGTAACCATCTAAAACAGCTAATTAATTAACAAAATGTTAATCTAATCTATTTATTTATTTACATTAATAATGGTGAATTGGTTATTGTTTTGTTGCAGGTTTCCTTCACTGTAGGTTTATTCTCAGCTAG
Protein sequence

>MS.gene43552.t1

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNNEDLNKVSSNILSAETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQDNLNVVLSSLKNVYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKMLKPMIEFLQTVNSTFSLIPSSMFSHFSDKGLNFVSSHLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPIPEIAQPPLDFPAGSPYPAPYNVPNLKPLPPLAQIVSSPPPTSSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAIENGSPSPSHYPQIAPVQKLWCVAKPSVPDETLQEALDYACGEGGADCLEITTPQGNCYNPDTLVAHASYAFNSYWQKHKRIGGTCDFGGTAMLIHSDPSFLHCRFILS